VPS53 cDNA ORF clone, Pteropus alecto(black flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS53
Entrez Gene ID	102884797
Full Name	VPS53, GARP complex subunit
Gene Type	protein-coding
Organism	Pteropus alecto(black flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025041785.1	XP_024897553.1	vacuolar protein sorting-associated protein 53 homolog isoform X2
XM_006925053.2	XP_006925115.1	vacuolar protein sorting-associated protein 53 homolog
XM_006925053.3	XP_006925115.1	vacuolar protein sorting-associated protein 53 homolog isoform X1
XM_025041786.1	XP_024897554.1	vacuolar protein sorting-associated protein 53 homolog isoform X3

Pathways from BioSystems
Gene Ontology

The following VPS53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt29949	XM_006925053.3 Latest version!	Pteropus alecto VPS53, GARP complex subunit (VPS53), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPt83849	XM_025041785.1 Latest version!	Pteropus alecto VPS53, GARP complex subunit (VPS53), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OPt83850	XM_025041786.1 Latest version!	Pteropus alecto VPS53, GARP complex subunit (VPS53), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OPt29949	XM_006925053.2	Pteropus alecto VPS53 GARP complex subunit (VPS53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS53 ( XM_006925053.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006925115.1 Pteropus alecto VPS53 GARP complex subunit (VPS53), mRNA.

Price & Availability↑

CloneID	OPt29949
Clone ID Related Accession (Same CDS sequence)	XM_006925053.2 , XM_006925053.3
Accession Version	XM_006925053.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	vacuolar protein sorting-associated protein 53 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006443570.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 10, 2016 this sequence version replaced XM_006925053.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAAGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGACGTGC AGCTCGCCAT CGAACAGGTA TTTCCAAGCC AGGATCCTTT AGATCGAGCA 
GATTTCAATG CTGTGGAGTA TATCAATGCC CTGTTCCCAA CGGAGCAATC TCTGGCAAAC 
ATAGATGAAG TTGTGAACAA GATTAGACTG AAAATAAGGA GACTAGATGA CAATATTCGA 
ACTGTCGTAA GAGGTCAAAC AAATGTGGGA CAGGATGGCA GACAAGCGCT TGAAGAGGCC 
CAGAAAGCAA TTCAGCAACT CTTCGGCAAA ATCAAAGATA TCAAAGACAA AGCAGAAAAA 
TCAGAGCAAA TGGTTAAAGA AATCACCCGT GATATTAAGC AGTTAGATCA TGCCAAACGC 
CACCTGACCA CCTCAATCAC AACGCTGAAC CACCTGCACA TGTTGGCAGG CGGCGTCGAT 
TCCCTCGAAG CCATGACCAG GCGAAGGCAA TATGGAGAAG TTGCTAATCT TCTTCAGGGT 
GTAATGAATG TTCTGGAACA CTTCCACAAG TATATGGGAA TTCCACAGAT CCGGCAGCTT 
TCTGAAAGAG TGAAGGCCGC CCAGACTGAG TTAGGACAGC AAATCCTGGC CGATTTTGAA 
GAAGCATTTC CTTCTCAGGG TACCAAGAGG CCCGGAGGAC CCAGCAATGT CCTTCGAGAT 
GCATGCCTGG TTGCTAATAT TTTGGACCCC AGAATCAAAC AGGATATCAT CAAAAAGTTT 
ATTAAACAGC ATCTGTCAGA GTATCTAGTA CTTTTTCAAG AAAACCAAGA TGTCGCCTGG 
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG 
AAATATGGCC GAATGTTTCC ACGTGAGTGG TACATGACCG AGAGGATTGC AGTAGAATTT 
TGCCACGTCA CGAGGACAGA ACTTGCCAAG ATTATGCGAA CCAGAGCTAA GGAAATTGAA 
GTGAAGTTGC TTCTTTTTGC TATTCAGAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA 
CGTTTCTCCG GCTGCACCCT GACAGATGGA ACCCTGAAAA AACTTGACTC TCCACCCCCA 
TCCACCAATC CCTTCTTGGA AGATGAGCCA GCACCAGAGA TGGAGGAACT GGCAATGGAG 
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT 
GTTTCCAAGT GTTTTGAGCC TCATCTTTAC GTGTATATTG AGTCCCAGGA CAAAAACCTT 
GGAGAGCTGA TAGATCGGTT TGTGGCCGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC 
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCTGACC TCTTTGTCTA CTACAAGAAG 
TGCATGGTGC AGTGCTCCCA ACTCAGTACT GGGGAGCCGA TGATCGCTTT GACCACCATT 
TTCCAGAAGT ACCTTCGAGA ATATGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC 
ACAAGCAGCA GTGGAGGGCT GACCATCAGC AGCCTCCTCA AGGAAAAGGA AGGCTCAGAA 
GTGGCCAAGT TCACTCTAGA GGAGCTCTGC CTCATCTGCA GCATCCTGAG CACAGCAGAG 
TACTGTCTGG CCACCACTCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT TGATGTAAGT 
CTGATCGAAC GAATCAATCT GACTGGAGAA ATGGACACAT TCAGCACTGT CATCTCTAGC 
AGTATTCAGT TGCTGGTTCA GGATTTGGAT GCTGCCTGCG ATCCTGCCCT AACTGCCATG 
AGCAAGATGC AGTGGCAGAA TGTGGAACAT GTTGGTGACC AGAGCCCCTA CGTCACCTCT 
GTCATTCTTC ACATTAAGCA AAACGTCCCC ATCATCCGTG ACAACCTGGC TTCGACGCGA 
AAATACTTCA CTCAGTTCTG CATTAAATTT GCAAACTCCT TCATTCCCAA GTTCATCACC 
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGCTGCT ACTGGACACC 
CATTCCCTGA AGATGGTCCT GCTGGATCTA CCTTCCATTG GCTCACAGGT GGTGAGGAAA 
GCACCTGCCA GCTACACCAA GATTGTTGTG AAAGGCATGA CCCGGGCGGA GATGATCCTC 
AAGGTAGTGA TGGCCCCCCA CGAACCGTCC GTGGTATTTG TAGACAACTA CATCAAACTC 
CTTACGGACT GCAATACAGA AACCTTCCAG AAGATACTGG ACATGAAGGG GCTGAAGAGG 
AGTGAGCAGA GCTGCATGCT GGAGCTCTTT CGCCAGAGGC TCCCCACCCC GCCCTCAGGG 
CCAGAAGGCT CCAGCTCCTT GTCCCTACTG GCTCCCACAC CAGAACAGGA ATCATCTCGC 
ATCCGCAAAC TAGAGAAACT AATTAAGAAG AGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006925115.1
CDS	133..2631
Translation	MMEEEELEFVEELEAVLQLTPDVQLAIEQVFPSQDPLDRADFNAVEYINALFPTEQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQDIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWYMTERIAVEFCHVTRTELAKIMRTRAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLDSPPPSTNPFLEDEPAPEMEELAMEKGDLDQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTSSSGGLTISSLLKEKEGSEVAKFTLEELCLICSILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCIKFANSFIPKFITHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSIGSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPSVVFVDNYIKLLTDCNTETFQKILDMKGLKRSEQSCMLELFRQRLPTPPSGPEGSSSLSLLAPTPEQESSRIRKLEKLIKKRL

Target ORF information:

RefSeq Version	XM_006925053.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto VPS53 GARP complex subunit (VPS53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006925053.2

ORF Insert Sequence:

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAAGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGACGTGC AGCTCGCCAT CGAACAGGTA TTTCCAAGCC AGGATCCTTT AGATCGAGCA 
GATTTCAATG CTGTGGAGTA TATCAATGCC CTGTTCCCAA CGGAGCAATC TCTGGCAAAC 
ATAGATGAAG TTGTGAACAA GATTAGACTG AAAATAAGGA GACTAGATGA CAATATTCGA 
ACTGTCGTAA GAGGTCAAAC AAATGTGGGA CAGGATGGCA GACAAGCGCT TGAAGAGGCC 
CAGAAAGCAA TTCAGCAACT CTTCGGCAAA ATCAAAGATA TCAAAGACAA AGCAGAAAAA 
TCAGAGCAAA TGGTTAAAGA AATCACCCGT GATATTAAGC AGTTAGATCA TGCCAAACGC 
CACCTGACCA CCTCAATCAC AACGCTGAAC CACCTGCACA TGTTGGCAGG CGGCGTCGAT 
TCCCTCGAAG CCATGACCAG GCGAAGGCAA TATGGAGAAG TTGCTAATCT TCTTCAGGGT 
GTAATGAATG TTCTGGAACA CTTCCACAAG TATATGGGAA TTCCACAGAT CCGGCAGCTT 
TCTGAAAGAG TGAAGGCCGC CCAGACTGAG TTAGGACAGC AAATCCTGGC CGATTTTGAA 
GAAGCATTTC CTTCTCAGGG TACCAAGAGG CCCGGAGGAC CCAGCAATGT CCTTCGAGAT 
GCATGCCTGG TTGCTAATAT TTTGGACCCC AGAATCAAAC AGGATATCAT CAAAAAGTTT 
ATTAAACAGC ATCTGTCAGA GTATCTAGTA CTTTTTCAAG AAAACCAAGA TGTCGCCTGG 
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG 
AAATATGGCC GAATGTTTCC ACGTGAGTGG TACATGACCG AGAGGATTGC AGTAGAATTT 
TGCCACGTCA CGAGGACAGA ACTTGCCAAG ATTATGCGAA CCAGAGCTAA GGAAATTGAA 
GTGAAGTTGC TTCTTTTTGC TATTCAGAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA 
CGTTTCTCCG GCTGCACCCT GACAGATGGA ACCCTGAAAA AACTTGACTC TCCACCCCCA 
TCCACCAATC CCTTCTTGGA AGATGAGCCA GCACCAGAGA TGGAGGAACT GGCAATGGAG 
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT 
GTTTCCAAGT GTTTTGAGCC TCATCTTTAC GTGTATATTG AGTCCCAGGA CAAAAACCTT 
GGAGAGCTGA TAGATCGGTT TGTGGCCGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC 
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCTGACC TCTTTGTCTA CTACAAGAAG 
TGCATGGTGC AGTGCTCCCA ACTCAGTACT GGGGAGCCGA TGATCGCTTT GACCACCATT 
TTCCAGAAGT ACCTTCGAGA ATATGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC 
ACAAGCAGCA GTGGAGGGCT GACCATCAGC AGCCTCCTCA AGGAAAAGGA AGGCTCAGAA 
GTGGCCAAGT TCACTCTAGA GGAGCTCTGC CTCATCTGCA GCATCCTGAG CACAGCAGAG 
TACTGTCTGG CCACCACTCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT TGATGTAAGT 
CTGATCGAAC GAATCAATCT GACTGGAGAA ATGGACACAT TCAGCACTGT CATCTCTAGC 
AGTATTCAGT TGCTGGTTCA GGATTTGGAT GCTGCCTGCG ATCCTGCCCT AACTGCCATG 
AGCAAGATGC AGTGGCAGAA TGTGGAACAT GTTGGTGACC AGAGCCCCTA CGTCACCTCT 
GTCATTCTTC ACATTAAGCA AAACGTCCCC ATCATCCGTG ACAACCTGGC TTCGACGCGA 
AAATACTTCA CTCAGTTCTG CATTAAATTT GCAAACTCCT TCATTCCCAA GTTCATCACC 
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGCTGCT ACTGGACACC 
CATTCCCTGA AGATGGTCCT GCTGGATCTA CCTTCCATTG GCTCACAGGT GGTGAGGAAA 
GCACCTGCCA GCTACACCAA GATTGTTGTG AAAGGCATGA CCCGGGCGGA GATGATCCTC 
AAGGTAGTGA TGGCCCCCCA CGAACCGTCC GTGGTATTTG TAGACAACTA CATCAAACTC 
CTTACGGACT GCAATACAGA AACCTTCCAG AAGATACTGG ACATGAAGGG GCTGAAGAGG 
AGTGAGCAGA GCTGCATGCT GGAGCTCTTT CGCCAGAGGC TCCCCACCCC GCCCTCAGGG 
CCAGAAGGCT CCAGCTCCTT GTCCCTACTG GCTCCCACAC CAGAACAGGA ATCATCTCGC 
ATCCGCAAAC TAGAGAAACT AATTAAGAAG AGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS53 ( XM_006925053.3 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006925115.1 Pteropus alecto VPS53, GARP complex subunit (VPS53), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPt29949
Clone ID Related Accession (Same CDS sequence)	XM_006925053.2 , XM_006925053.3
Accession Version	XM_006925053.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	vacuolar protein sorting-associated protein 53 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006443570.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_006925053.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAAGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGACGTGC AGCTCGCCAT CGAACAGGTA TTTCCAAGCC AGGATCCTTT AGATCGAGCA 
GATTTCAATG CTGTGGAGTA TATCAATGCC CTGTTCCCAA CGGAGCAATC TCTGGCAAAC 
ATAGATGAAG TTGTGAACAA GATTAGACTG AAAATAAGGA GACTAGATGA CAATATTCGA 
ACTGTCGTAA GAGGTCAAAC AAATGTGGGA CAGGATGGCA GACAAGCGCT TGAAGAGGCC 
CAGAAAGCAA TTCAGCAACT CTTCGGCAAA ATCAAAGATA TCAAAGACAA AGCAGAAAAA 
TCAGAGCAAA TGGTTAAAGA AATCACCCGT GATATTAAGC AGTTAGATCA TGCCAAACGC 
CACCTGACCA CCTCAATCAC AACGCTGAAC CACCTGCACA TGTTGGCAGG CGGCGTCGAT 
TCCCTCGAAG CCATGACCAG GCGAAGGCAA TATGGAGAAG TTGCTAATCT TCTTCAGGGT 
GTAATGAATG TTCTGGAACA CTTCCACAAG TATATGGGAA TTCCACAGAT CCGGCAGCTT 
TCTGAAAGAG TGAAGGCCGC CCAGACTGAG TTAGGACAGC AAATCCTGGC CGATTTTGAA 
GAAGCATTTC CTTCTCAGGG TACCAAGAGG CCCGGAGGAC CCAGCAATGT CCTTCGAGAT 
GCATGCCTGG TTGCTAATAT TTTGGACCCC AGAATCAAAC AGGATATCAT CAAAAAGTTT 
ATTAAACAGC ATCTGTCAGA GTATCTAGTA CTTTTTCAAG AAAACCAAGA TGTCGCCTGG 
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG 
AAATATGGCC GAATGTTTCC ACGTGAGTGG TACATGACCG AGAGGATTGC AGTAGAATTT 
TGCCACGTCA CGAGGACAGA ACTTGCCAAG ATTATGCGAA CCAGAGCTAA GGAAATTGAA 
GTGAAGTTGC TTCTTTTTGC TATTCAGAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA 
CGTTTCTCCG GCTGCACCCT GACAGATGGA ACCCTGAAAA AACTTGACTC TCCACCCCCA 
TCCACCAATC CCTTCTTGGA AGATGAGCCA GCACCAGAGA TGGAGGAACT GGCAATGGAG 
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT 
GTTTCCAAGT GTTTTGAGCC TCATCTTTAC GTGTATATTG AGTCCCAGGA CAAAAACCTT 
GGAGAGCTGA TAGATCGGTT TGTGGCCGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC 
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCTGACC TCTTTGTCTA CTACAAGAAG 
TGCATGGTGC AGTGCTCCCA ACTCAGTACT GGGGAGCCGA TGATCGCTTT GACCACCATT 
TTCCAGAAGT ACCTTCGAGA ATATGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC 
ACAAGCAGCA GTGGAGGGCT GACCATCAGC AGCCTCCTCA AGGAAAAGGA AGGCTCAGAA 
GTGGCCAAGT TCACTCTAGA GGAGCTCTGC CTCATCTGCA GCATCCTGAG CACAGCAGAG 
TACTGTCTGG CCACCACTCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT TGATGTAAGT 
CTGATCGAAC GAATCAATCT GACTGGAGAA ATGGACACAT TCAGCACTGT CATCTCTAGC 
AGTATTCAGT TGCTGGTTCA GGATTTGGAT GCTGCCTGCG ATCCTGCCCT AACTGCCATG 
AGCAAGATGC AGTGGCAGAA TGTGGAACAT GTTGGTGACC AGAGCCCCTA CGTCACCTCT 
GTCATTCTTC ACATTAAGCA AAACGTCCCC ATCATCCGTG ACAACCTGGC TTCGACGCGA 
AAATACTTCA CTCAGTTCTG CATTAAATTT GCAAACTCCT TCATTCCCAA GTTCATCACC 
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGCTGCT ACTGGACACC 
CATTCCCTGA AGATGGTCCT GCTGGATCTA CCTTCCATTG GCTCACAGGT GGTGAGGAAA 
GCACCTGCCA GCTACACCAA GATTGTTGTG AAAGGCATGA CCCGGGCGGA GATGATCCTC 
AAGGTAGTGA TGGCCCCCCA CGAACCGTCC GTGGTATTTG TAGACAACTA CATCAAACTC 
CTTACGGACT GCAATACAGA AACCTTCCAG AAGATACTGG ACATGAAGGG GCTGAAGAGG 
AGTGAGCAGA GCTGCATGCT GGAGCTCTTT CGCCAGAGGC TCCCCACCCC GCCCTCAGGG 
CCAGAAGGCT CCAGCTCCTT GTCCCTACTG GCTCCCACAC CAGAACAGGA ATCATCTCGC 
ATCCGCAAAC TAGAGAAACT AATTAAGAAG AGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006925115.1
CDS	155..2653
Translation	MMEEEELEFVEELEAVLQLTPDVQLAIEQVFPSQDPLDRADFNAVEYINALFPTEQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQDIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWYMTERIAVEFCHVTRTELAKIMRTRAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLDSPPPSTNPFLEDEPAPEMEELAMEKGDLDQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTSSSGGLTISSLLKEKEGSEVAKFTLEELCLICSILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCIKFANSFIPKFITHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSIGSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPSVVFVDNYIKLLTDCNTETFQKILDMKGLKRSEQSCMLELFRQRLPTPPSGPEGSSSLSLLAPTPEQESSRIRKLEKLIKKRL

Target ORF information:

RefSeq Version	XM_006925053.3
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto VPS53, GARP complex subunit (VPS53), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006925053.3

ORF Insert Sequence:

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAAGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGACGTGC AGCTCGCCAT CGAACAGGTA TTTCCAAGCC AGGATCCTTT AGATCGAGCA 
GATTTCAATG CTGTGGAGTA TATCAATGCC CTGTTCCCAA CGGAGCAATC TCTGGCAAAC 
ATAGATGAAG TTGTGAACAA GATTAGACTG AAAATAAGGA GACTAGATGA CAATATTCGA 
ACTGTCGTAA GAGGTCAAAC AAATGTGGGA CAGGATGGCA GACAAGCGCT TGAAGAGGCC 
CAGAAAGCAA TTCAGCAACT CTTCGGCAAA ATCAAAGATA TCAAAGACAA AGCAGAAAAA 
TCAGAGCAAA TGGTTAAAGA AATCACCCGT GATATTAAGC AGTTAGATCA TGCCAAACGC 
CACCTGACCA CCTCAATCAC AACGCTGAAC CACCTGCACA TGTTGGCAGG CGGCGTCGAT 
TCCCTCGAAG CCATGACCAG GCGAAGGCAA TATGGAGAAG TTGCTAATCT TCTTCAGGGT 
GTAATGAATG TTCTGGAACA CTTCCACAAG TATATGGGAA TTCCACAGAT CCGGCAGCTT 
TCTGAAAGAG TGAAGGCCGC CCAGACTGAG TTAGGACAGC AAATCCTGGC CGATTTTGAA 
GAAGCATTTC CTTCTCAGGG TACCAAGAGG CCCGGAGGAC CCAGCAATGT CCTTCGAGAT 
GCATGCCTGG TTGCTAATAT TTTGGACCCC AGAATCAAAC AGGATATCAT CAAAAAGTTT 
ATTAAACAGC ATCTGTCAGA GTATCTAGTA CTTTTTCAAG AAAACCAAGA TGTCGCCTGG 
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG 
AAATATGGCC GAATGTTTCC ACGTGAGTGG TACATGACCG AGAGGATTGC AGTAGAATTT 
TGCCACGTCA CGAGGACAGA ACTTGCCAAG ATTATGCGAA CCAGAGCTAA GGAAATTGAA 
GTGAAGTTGC TTCTTTTTGC TATTCAGAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA 
CGTTTCTCCG GCTGCACCCT GACAGATGGA ACCCTGAAAA AACTTGACTC TCCACCCCCA 
TCCACCAATC CCTTCTTGGA AGATGAGCCA GCACCAGAGA TGGAGGAACT GGCAATGGAG 
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT 
GTTTCCAAGT GTTTTGAGCC TCATCTTTAC GTGTATATTG AGTCCCAGGA CAAAAACCTT 
GGAGAGCTGA TAGATCGGTT TGTGGCCGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC 
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCTGACC TCTTTGTCTA CTACAAGAAG 
TGCATGGTGC AGTGCTCCCA ACTCAGTACT GGGGAGCCGA TGATCGCTTT GACCACCATT 
TTCCAGAAGT ACCTTCGAGA ATATGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC 
ACAAGCAGCA GTGGAGGGCT GACCATCAGC AGCCTCCTCA AGGAAAAGGA AGGCTCAGAA 
GTGGCCAAGT TCACTCTAGA GGAGCTCTGC CTCATCTGCA GCATCCTGAG CACAGCAGAG 
TACTGTCTGG CCACCACTCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT TGATGTAAGT 
CTGATCGAAC GAATCAATCT GACTGGAGAA ATGGACACAT TCAGCACTGT CATCTCTAGC 
AGTATTCAGT TGCTGGTTCA GGATTTGGAT GCTGCCTGCG ATCCTGCCCT AACTGCCATG 
AGCAAGATGC AGTGGCAGAA TGTGGAACAT GTTGGTGACC AGAGCCCCTA CGTCACCTCT 
GTCATTCTTC ACATTAAGCA AAACGTCCCC ATCATCCGTG ACAACCTGGC TTCGACGCGA 
AAATACTTCA CTCAGTTCTG CATTAAATTT GCAAACTCCT TCATTCCCAA GTTCATCACC 
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGCTGCT ACTGGACACC 
CATTCCCTGA AGATGGTCCT GCTGGATCTA CCTTCCATTG GCTCACAGGT GGTGAGGAAA 
GCACCTGCCA GCTACACCAA GATTGTTGTG AAAGGCATGA CCCGGGCGGA GATGATCCTC 
AAGGTAGTGA TGGCCCCCCA CGAACCGTCC GTGGTATTTG TAGACAACTA CATCAAACTC 
CTTACGGACT GCAATACAGA AACCTTCCAG AAGATACTGG ACATGAAGGG GCTGAAGAGG 
AGTGAGCAGA GCTGCATGCT GGAGCTCTTT CGCCAGAGGC TCCCCACCCC GCCCTCAGGG 
CCAGAAGGCT CCAGCTCCTT GTCCCTACTG GCTCCCACAC CAGAACAGGA ATCATCTCGC 
ATCCGCAAAC TAGAGAAACT AATTAAGAAG AGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS53 ( XM_025041785.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_024897553.1 Pteropus alecto VPS53, GARP complex subunit (VPS53), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPt83849
Clone ID Related Accession (Same CDS sequence)	XM_025041785.1
Accession Version	XM_025041785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	vacuolar protein sorting-associated protein 53 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006443570.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAAGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGACGTGC AGCTCGCCAT CGAACAGGTA TTTCCAAGCC AGGATCCTTT AGATCGAGCA 
GATTTCAATG CTGTGGAGTA TATCAATGCC CTGTTCCCAA CGGAGCAATC TCTGGCAAAC 
ATAGATGAAG TTGTGAACAA GATTAGACTG AAAATAAGGA GACTAGATGA CAATATTCGA 
ACTGTCGTAA GAGGTCAAAC AAATGTGGGA CAGGATGGCA GACAAGCGCT TGAAGAGGCC 
CAGAAAGCAA TTCAGCAACT CTTCGGCAAA ATCAAAGATA TCAAAGACAA AGCAGAAAAA 
TCAGAGCAAA TGGTTAAAGA AATCACCCGT GATATTAAGC AGTTAGATCA TGCCAAACGC 
CACCTGACCA CCTCAATCAC AACGCTGAAC CACCTGCACA TGTTGGCAGG CGGCGTCGAT 
TCCCTCGAAG CCATGACCAG GCGAAGGCAA TATGGAGAAG TTGCTAATCT TCTTCAGGGT 
GTAATGAATG TTCTGGAACA CTTCCACAAG TATATGGGAA TTCCACAGAT CCGGCAGCTT 
TCTGAAAGAG TGAAGGCCGC CCAGACTGAG TTAGGACAGC AAATCCTGGC CGATTTTGAA 
GAAGCATTTC CTTCTCAGGG TACCAAGAGG CCCGGAGGAC CCAGCAATGT CCTTCGAGAT 
GCATGCCTGG TTGCTAATAT TTTGGACCCC AGAATCAAAC AGGATATCAT CAAAAAGTTT 
ATTAAACAGC ATCTGTCAGA GTATCTAGTA CTTTTTCAAG AAAACCAAGA TGTCGCCTGG 
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG 
AAATATGGCC GAATGTTTCC ACGTGAGTGG TACATGACCG AGAGGATTGC AGTAGAATTT 
TGCCACGTCA CGAGGACAGA ACTTGCCAAG ATTATGCGAA CCAGAGCTAA GGAAATTGAA 
GTGAAGTTGC TTCTTTTTGC TATTCAGAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA 
CGTTTCTCCG GCTGCACCCT GACAGATGGA ACCCTGCCAA AGAAGCCTAA AGCCCCAGAC 
AATCCATTTC ATGGCATTGT TTCCAAGTGT TTTGAGCCTC ATCTTTACGT GTATATTGAG 
TCCCAGGACA AAAACCTTGG AGAGCTGATA GATCGGTTTG TGGCCGATTT CAAAGCCCAG 
GGGCCACCTA AGCCCAACAC TGATGAAGGG GGTGCCGTGC TCCCCAGCTG CGCTGACCTC 
TTTGTCTACT ACAAGAAGTG CATGGTGCAG TGCTCCCAAC TCAGTACTGG GGAGCCGATG 
ATCGCTTTGA CCACCATTTT CCAGAAGTAC CTTCGAGAAT ATGCCTGGAA AATCCTCTCT 
GGCAACCTGC CCAAAACCAC AAGCAGCAGT GGAGGGCTGA CCATCAGCAG CCTCCTCAAG 
GAAAAGGAAG GCTCAGAAGT GGCCAAGTTC ACTCTAGAGG AGCTCTGCCT CATCTGCAGC 
ATCCTGAGCA CAGCAGAGTA CTGTCTGGCC ACCACTCAGC AGCTAGAAGA AAAACTCAAA 
GAAAAAGTTG ATGTAAGTCT GATCGAACGA ATCAATCTGA CTGGAGAAAT GGACACATTC 
AGCACTGTCA TCTCTAGCAG TATTCAGTTG CTGGTTCAGG ATTTGGATGC TGCCTGCGAT 
CCTGCCCTAA CTGCCATGAG CAAGATGCAG TGGCAGAATG TGGAACATGT TGGTGACCAG 
AGCCCCTACG TCACCTCTGT CATTCTTCAC ATTAAGCAAA ACGTCCCCAT CATCCGTGAC 
AACCTGGCTT CGACGCGAAA ATACTTCACT CAGTTCTGCA TTAAATTTGC AAACTCCTTC 
ATTCCCAAGT TCATCACCCA CCTCTTCAAG TGCAAGCCAA TTAGCATGGT GGGAGCAGAA 
CAGCTGCTAC TGGACACCCA TTCCCTGAAG ATGGTCCTGC TGGATCTACC TTCCATTGGC 
TCACAGGTGG TGAGGAAAGC ACCTGCCAGC TACACCAAGA TTGTTGTGAA AGGCATGACC 
CGGGCGGAGA TGATCCTCAA GGTAGTGATG GCCCCCCACG AACCGTCCGT GGTATTTGTA 
GACAACTACA TCAAACTCCT TACGGACTGC AATACAGAAA CCTTCCAGAA GATACTGGAC 
ATGAAGGGGC TGAAGAGGAG TGAGCAGAGC TGCATGCTGG AGCTCTTTCG CCAGAGGCTC 
CCCACCCCGC CCTCAGGGCC AGAAGGCTCC AGCTCCTTGT CCCTACTGGC TCCCACACCA 
GAACAGGAAT CATCTCGCAT CCGCAAACTA GAGAAACTAA TTAAGAAGAG ACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024897553.1
CDS	155..2551
Translation	MMEEEELEFVEELEAVLQLTPDVQLAIEQVFPSQDPLDRADFNAVEYINALFPTEQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQDIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWYMTERIAVEFCHVTRTELAKIMRTRAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTSSSGGLTISSLLKEKEGSEVAKFTLEELCLICSILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCIKFANSFIPKFITHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSIGSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPSVVFVDNYIKLLTDCNTETFQKILDMKGLKRSEQSCMLELFRQRLPTPPSGPEGSSSLSLLAPTPEQESSRIRKLEKLIKKRL

Target ORF information:

RefSeq Version	XM_025041785.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto VPS53, GARP complex subunit (VPS53), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025041785.1

ORF Insert Sequence:

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAAGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGACGTGC AGCTCGCCAT CGAACAGGTA TTTCCAAGCC AGGATCCTTT AGATCGAGCA 
GATTTCAATG CTGTGGAGTA TATCAATGCC CTGTTCCCAA CGGAGCAATC TCTGGCAAAC 
ATAGATGAAG TTGTGAACAA GATTAGACTG AAAATAAGGA GACTAGATGA CAATATTCGA 
ACTGTCGTAA GAGGTCAAAC AAATGTGGGA CAGGATGGCA GACAAGCGCT TGAAGAGGCC 
CAGAAAGCAA TTCAGCAACT CTTCGGCAAA ATCAAAGATA TCAAAGACAA AGCAGAAAAA 
TCAGAGCAAA TGGTTAAAGA AATCACCCGT GATATTAAGC AGTTAGATCA TGCCAAACGC 
CACCTGACCA CCTCAATCAC AACGCTGAAC CACCTGCACA TGTTGGCAGG CGGCGTCGAT 
TCCCTCGAAG CCATGACCAG GCGAAGGCAA TATGGAGAAG TTGCTAATCT TCTTCAGGGT 
GTAATGAATG TTCTGGAACA CTTCCACAAG TATATGGGAA TTCCACAGAT CCGGCAGCTT 
TCTGAAAGAG TGAAGGCCGC CCAGACTGAG TTAGGACAGC AAATCCTGGC CGATTTTGAA 
GAAGCATTTC CTTCTCAGGG TACCAAGAGG CCCGGAGGAC CCAGCAATGT CCTTCGAGAT 
GCATGCCTGG TTGCTAATAT TTTGGACCCC AGAATCAAAC AGGATATCAT CAAAAAGTTT 
ATTAAACAGC ATCTGTCAGA GTATCTAGTA CTTTTTCAAG AAAACCAAGA TGTCGCCTGG 
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG 
AAATATGGCC GAATGTTTCC ACGTGAGTGG TACATGACCG AGAGGATTGC AGTAGAATTT 
TGCCACGTCA CGAGGACAGA ACTTGCCAAG ATTATGCGAA CCAGAGCTAA GGAAATTGAA 
GTGAAGTTGC TTCTTTTTGC TATTCAGAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA 
CGTTTCTCCG GCTGCACCCT GACAGATGGA ACCCTGCCAA AGAAGCCTAA AGCCCCAGAC 
AATCCATTTC ATGGCATTGT TTCCAAGTGT TTTGAGCCTC ATCTTTACGT GTATATTGAG 
TCCCAGGACA AAAACCTTGG AGAGCTGATA GATCGGTTTG TGGCCGATTT CAAAGCCCAG 
GGGCCACCTA AGCCCAACAC TGATGAAGGG GGTGCCGTGC TCCCCAGCTG CGCTGACCTC 
TTTGTCTACT ACAAGAAGTG CATGGTGCAG TGCTCCCAAC TCAGTACTGG GGAGCCGATG 
ATCGCTTTGA CCACCATTTT CCAGAAGTAC CTTCGAGAAT ATGCCTGGAA AATCCTCTCT 
GGCAACCTGC CCAAAACCAC AAGCAGCAGT GGAGGGCTGA CCATCAGCAG CCTCCTCAAG 
GAAAAGGAAG GCTCAGAAGT GGCCAAGTTC ACTCTAGAGG AGCTCTGCCT CATCTGCAGC 
ATCCTGAGCA CAGCAGAGTA CTGTCTGGCC ACCACTCAGC AGCTAGAAGA AAAACTCAAA 
GAAAAAGTTG ATGTAAGTCT GATCGAACGA ATCAATCTGA CTGGAGAAAT GGACACATTC 
AGCACTGTCA TCTCTAGCAG TATTCAGTTG CTGGTTCAGG ATTTGGATGC TGCCTGCGAT 
CCTGCCCTAA CTGCCATGAG CAAGATGCAG TGGCAGAATG TGGAACATGT TGGTGACCAG 
AGCCCCTACG TCACCTCTGT CATTCTTCAC ATTAAGCAAA ACGTCCCCAT CATCCGTGAC 
AACCTGGCTT CGACGCGAAA ATACTTCACT CAGTTCTGCA TTAAATTTGC AAACTCCTTC 
ATTCCCAAGT TCATCACCCA CCTCTTCAAG TGCAAGCCAA TTAGCATGGT GGGAGCAGAA 
CAGCTGCTAC TGGACACCCA TTCCCTGAAG ATGGTCCTGC TGGATCTACC TTCCATTGGC 
TCACAGGTGG TGAGGAAAGC ACCTGCCAGC TACACCAAGA TTGTTGTGAA AGGCATGACC 
CGGGCGGAGA TGATCCTCAA GGTAGTGATG GCCCCCCACG AACCGTCCGT GGTATTTGTA 
GACAACTACA TCAAACTCCT TACGGACTGC AATACAGAAA CCTTCCAGAA GATACTGGAC 
ATGAAGGGGC TGAAGAGGAG TGAGCAGAGC TGCATGCTGG AGCTCTTTCG CCAGAGGCTC 
CCCACCCCGC CCTCAGGGCC AGAAGGCTCC AGCTCCTTGT CCCTACTGGC TCCCACACCA 
GAACAGGAAT CATCTCGCAT CCGCAAACTA GAGAAACTAA TTAAGAAGAG ACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS53 ( XM_025041786.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_024897554.1 Pteropus alecto VPS53, GARP complex subunit (VPS53), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPt83850
Clone ID Related Accession (Same CDS sequence)	XM_025041786.1
Accession Version	XM_025041786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	vacuolar protein sorting-associated protein 53 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006443570.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTAAAG AAATCACCCG TGATATTAAG CAGTTAGATC ATGCCAAACG CCACCTGACC 
ACCTCAATCA CAACGCTGAA CCACCTGCAC ATGTTGGCAG GCGGCGTCGA TTCCCTCGAA 
GCCATGACCA GGCGAAGGCA ATATGGAGAA GTTGCTAATC TTCTTCAGGG TGTAATGAAT 
GTTCTGGAAC ACTTCCACAA GTATATGGGA ATTCCACAGA TCCGGCAGCT TTCTGAAAGA 
GTGAAGGCCG CCCAGACTGA GTTAGGACAG CAAATCCTGG CCGATTTTGA AGAAGCATTT 
CCTTCTCAGG GTACCAAGAG GCCCGGAGGA CCCAGCAATG TCCTTCGAGA TGCATGCCTG 
GTTGCTAATA TTTTGGACCC CAGAATCAAA CAGGATATCA TCAAAAAGTT TATTAAACAG 
CATCTGTCAG AGTATCTAGT ACTTTTTCAA GAAAACCAAG ATGTCGCCTG GCTGGACAAA 
ATCGACAGAC GCTATGCCTG GATAAAACGC CAGCTTGTGG ACTATGAGGA GAAATATGGC 
CGAATGTTTC CACGTGAGTG GTACATGACC GAGAGGATTG CAGTAGAATT TTGCCACGTC 
ACGAGGACAG AACTTGCCAA GATTATGCGA ACCAGAGCTA AGGAAATTGA AGTGAAGTTG 
CTTCTTTTTG CTATTCAGAG AACAACTAAC TTTGAGGGGT TTCTTGCAAA ACGTTTCTCC 
GGCTGCACCC TGACAGATGG AACCCTGAAA AAACTTGACT CTCCACCCCC ATCCACCAAT 
CCCTTCTTGG AAGATGAGCC AGCACCAGAG ATGGAGGAAC TGGCAATGGA GAAAGGAGAT 
TTAGATCAAC CAAAGAAGCC TAAAGCCCCA GACAATCCAT TTCATGGCAT TGTTTCCAAG 
TGTTTTGAGC CTCATCTTTA CGTGTATATT GAGTCCCAGG ACAAAAACCT TGGAGAGCTG 
ATAGATCGGT TTGTGGCCGA TTTCAAAGCC CAGGGGCCAC CTAAGCCCAA CACTGATGAA 
GGGGGTGCCG TGCTCCCCAG CTGCGCTGAC CTCTTTGTCT ACTACAAGAA GTGCATGGTG 
CAGTGCTCCC AACTCAGTAC TGGGGAGCCG ATGATCGCTT TGACCACCAT TTTCCAGAAG 
TACCTTCGAG AATATGCCTG GAAAATCCTC TCTGGCAACC TGCCCAAAAC CACAAGCAGC 
AGTGGAGGGC TGACCATCAG CAGCCTCCTC AAGGAAAAGG AAGGCTCAGA AGTGGCCAAG 
TTCACTCTAG AGGAGCTCTG CCTCATCTGC AGCATCCTGA GCACAGCAGA GTACTGTCTG 
GCCACCACTC AGCAGCTAGA AGAAAAACTC AAAGAAAAAG TTGATGTAAG TCTGATCGAA 
CGAATCAATC TGACTGGAGA AATGGACACA TTCAGCACTG TCATCTCTAG CAGTATTCAG 
TTGCTGGTTC AGGATTTGGA TGCTGCCTGC GATCCTGCCC TAACTGCCAT GAGCAAGATG 
CAGTGGCAGA ATGTGGAACA TGTTGGTGAC CAGAGCCCCT ACGTCACCTC TGTCATTCTT 
CACATTAAGC AAAACGTCCC CATCATCCGT GACAACCTGG CTTCGACGCG AAAATACTTC 
ACTCAGTTCT GCATTAAATT TGCAAACTCC TTCATTCCCA AGTTCATCAC CCACCTCTTC 
AAGTGCAAGC CAATTAGCAT GGTGGGAGCA GAACAGCTGC TACTGGACAC CCATTCCCTG 
AAGATGGTCC TGCTGGATCT ACCTTCCATT GGCTCACAGG TGGTGAGGAA AGCACCTGCC 
AGCTACACCA AGATTGTTGT GAAAGGCATG ACCCGGGCGG AGATGATCCT CAAGGTAGTG 
ATGGCCCCCC ACGAACCGTC CGTGGTATTT GTAGACAACT ACATCAAACT CCTTACGGAC 
TGCAATACAG AAACCTTCCA GAAGATACTG GACATGAAGG GGCTGAAGAG GAGTGAGCAG 
AGCTGCATGC TGGAGCTCTT TCGCCAGAGG CTCCCCACCC CGCCCTCAGG GCCAGAAGGC 
TCCAGCTCCT TGTCCCTACT GGCTCCCACA CCAGAACAGG AATCATCTCG CATCCGCAAA 
CTAGAGAAAC TAATTAAGAA GAGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024897554.1
CDS	867..2996
Translation	MVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQDIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWYMTERIAVEFCHVTRTELAKIMRTRAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLDSPPPSTNPFLEDEPAPEMEELAMEKGDLDQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTSSSGGLTISSLLKEKEGSEVAKFTLEELCLICSILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCIKFANSFIPKFITHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSIGSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPSVVFVDNYIKLLTDCNTETFQKILDMKGLKRSEQSCMLELFRQRLPTPPSGPEGSSSLSLLAPTPEQESSRIRKLEKLIKKRL

Target ORF information:

RefSeq Version	XM_025041786.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto VPS53, GARP complex subunit (VPS53), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025041786.1

ORF Insert Sequence:

ATGGTTAAAG AAATCACCCG TGATATTAAG CAGTTAGATC ATGCCAAACG CCACCTGACC 
ACCTCAATCA CAACGCTGAA CCACCTGCAC ATGTTGGCAG GCGGCGTCGA TTCCCTCGAA 
GCCATGACCA GGCGAAGGCA ATATGGAGAA GTTGCTAATC TTCTTCAGGG TGTAATGAAT 
GTTCTGGAAC ACTTCCACAA GTATATGGGA ATTCCACAGA TCCGGCAGCT TTCTGAAAGA 
GTGAAGGCCG CCCAGACTGA GTTAGGACAG CAAATCCTGG CCGATTTTGA AGAAGCATTT 
CCTTCTCAGG GTACCAAGAG GCCCGGAGGA CCCAGCAATG TCCTTCGAGA TGCATGCCTG 
GTTGCTAATA TTTTGGACCC CAGAATCAAA CAGGATATCA TCAAAAAGTT TATTAAACAG 
CATCTGTCAG AGTATCTAGT ACTTTTTCAA GAAAACCAAG ATGTCGCCTG GCTGGACAAA 
ATCGACAGAC GCTATGCCTG GATAAAACGC CAGCTTGTGG ACTATGAGGA GAAATATGGC 
CGAATGTTTC CACGTGAGTG GTACATGACC GAGAGGATTG CAGTAGAATT TTGCCACGTC 
ACGAGGACAG AACTTGCCAA GATTATGCGA ACCAGAGCTA AGGAAATTGA AGTGAAGTTG 
CTTCTTTTTG CTATTCAGAG AACAACTAAC TTTGAGGGGT TTCTTGCAAA ACGTTTCTCC 
GGCTGCACCC TGACAGATGG AACCCTGAAA AAACTTGACT CTCCACCCCC ATCCACCAAT 
CCCTTCTTGG AAGATGAGCC AGCACCAGAG ATGGAGGAAC TGGCAATGGA GAAAGGAGAT 
TTAGATCAAC CAAAGAAGCC TAAAGCCCCA GACAATCCAT TTCATGGCAT TGTTTCCAAG 
TGTTTTGAGC CTCATCTTTA CGTGTATATT GAGTCCCAGG ACAAAAACCT TGGAGAGCTG 
ATAGATCGGT TTGTGGCCGA TTTCAAAGCC CAGGGGCCAC CTAAGCCCAA CACTGATGAA 
GGGGGTGCCG TGCTCCCCAG CTGCGCTGAC CTCTTTGTCT ACTACAAGAA GTGCATGGTG 
CAGTGCTCCC AACTCAGTAC TGGGGAGCCG ATGATCGCTT TGACCACCAT TTTCCAGAAG 
TACCTTCGAG AATATGCCTG GAAAATCCTC TCTGGCAACC TGCCCAAAAC CACAAGCAGC 
AGTGGAGGGC TGACCATCAG CAGCCTCCTC AAGGAAAAGG AAGGCTCAGA AGTGGCCAAG 
TTCACTCTAG AGGAGCTCTG CCTCATCTGC AGCATCCTGA GCACAGCAGA GTACTGTCTG 
GCCACCACTC AGCAGCTAGA AGAAAAACTC AAAGAAAAAG TTGATGTAAG TCTGATCGAA 
CGAATCAATC TGACTGGAGA AATGGACACA TTCAGCACTG TCATCTCTAG CAGTATTCAG 
TTGCTGGTTC AGGATTTGGA TGCTGCCTGC GATCCTGCCC TAACTGCCAT GAGCAAGATG 
CAGTGGCAGA ATGTGGAACA TGTTGGTGAC CAGAGCCCCT ACGTCACCTC TGTCATTCTT 
CACATTAAGC AAAACGTCCC CATCATCCGT GACAACCTGG CTTCGACGCG AAAATACTTC 
ACTCAGTTCT GCATTAAATT TGCAAACTCC TTCATTCCCA AGTTCATCAC CCACCTCTTC 
AAGTGCAAGC CAATTAGCAT GGTGGGAGCA GAACAGCTGC TACTGGACAC CCATTCCCTG 
AAGATGGTCC TGCTGGATCT ACCTTCCATT GGCTCACAGG TGGTGAGGAA AGCACCTGCC 
AGCTACACCA AGATTGTTGT GAAAGGCATG ACCCGGGCGG AGATGATCCT CAAGGTAGTG 
ATGGCCCCCC ACGAACCGTC CGTGGTATTT GTAGACAACT ACATCAAACT CCTTACGGAC 
TGCAATACAG AAACCTTCCA GAAGATACTG GACATGAAGG GGCTGAAGAG GAGTGAGCAG 
AGCTGCATGC TGGAGCTCTT TCGCCAGAGG CTCCCCACCC CGCCCTCAGG GCCAGAAGGC 
TCCAGCTCCT TGTCCCTACT GGCTCCCACA CCAGAACAGG AATCATCTCG CATCCGCAAA 
CTAGAGAAAC TAATTAAGAA GAGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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VPS53 cDNA ORF clone, Pteropus alecto(black flying fox)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム