UBR2 cDNA ORF clone, Pteropus alecto(black flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBR2
Entrez Gene ID	102879635
Full Name	ubiquitin protein ligase E3 component n-recognin 2
Gene Type	protein-coding
Organism	Pteropus alecto(black flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006926549.2	XP_006926611.1	E3 ubiquitin-protein ligase UBR2 isoform X1
XM_006926550.2	XP_006926612.1	E3 ubiquitin-protein ligase UBR2 isoform X2
XM_015600427.1	XP_015455913.1	E3 ubiquitin-protein ligase UBR2 isoform X3
XM_015600427.2	XP_015455913.1	E3 ubiquitin-protein ligase UBR2 isoform X3
XM_006926551.2	XP_006926613.1	E3 ubiquitin-protein ligase UBR2 isoform X5
XM_006926550.3	XP_006926612.1	E3 ubiquitin-protein ligase UBR2 isoform X1
XM_015600428.1	XP_015455914.1	E3 ubiquitin-protein ligase UBR2 isoform X4
XM_006926549.3	XP_006926611.1	E3 ubiquitin-protein ligase UBR2 isoform X2

Pathways from BioSystems
Gene Ontology

The following UBR2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBR2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt31995	XM_006926551.2 Latest version!	Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPt31994	XM_015600428.1 Latest version!	Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPt31991	XM_006926549.3 Latest version!	Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPt31992	XM_006926550.3 Latest version!	Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPt31993	XM_015600427.2 Latest version!	Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
View Old Accession Versions >>
OPt31993	XM_015600427.1	Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPt31992	XM_006926550.2	Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPt31991	XM_006926549.2	Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBR2 ( XM_006926551.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006926613.1 Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPt31995
Clone ID Related Accession (Same CDS sequence)	XM_006926551.2
Accession Version	XM_006926551.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	E3 ubiquitin-protein ligase UBR2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 10, 2016 this sequence version replaced XM_006926551.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC GGAGGTGCCG GCCATCGACC GGAGTTTGCT GGCATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCT ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT ACAGGAGGAA 
GACATGCTGG CACAGCATGT TTTGTTGGGA CCAATGGAAT GGTACCTTTG TGGTGAAGAT 
CCTGCATTTG GATTTCCAAA ACTTGAACAA GCAAACAAAC CTTCTCATCT TTGTGGTCGT 
GTTTTTAAAG TAGGAGAACC CACATATTCT TGCAGAGACT GTGCAGTTGA TCCAACTTGT 
GTTTTATGCA TGGAGTGCTT TTTGGGAAGT ATTCACAAAG ACCATCGATA TAGGATGACA 
ACATCAGGAG GTGGAGGTTT CTGTGACTGT GGTGATACTG AAGCTTGGAA AGAGGGTCCT 
TACTGTCAAA AACATGAACT TAACACCTCT GAAATTGAGG AAGAAGAGGA TCCTCTTGTG 
CATATATCAG ATGTGATAGC AAGAACTTAC AACATTTTTG CTATTATGTT TCGGTATGCA 
GTGGAGATAC TAACCTGGGA AAAAGAAAAT GAATTGCCAG CAGATTTAGA GATGGTAGAG 
AAGAGTGACA CCTACTATTG CATGCTTTTT AATGATGAGG TTCATACCTA TGAACAAGTT 
ATTTATACTC TTCAGAAAGC TGTTAACTGT ACACAAAAAG AAGCCATTGG CTTTGCAACT 
ACAGTAGATC GAGACGGACG TAGGTCTGTT CGATATGGAG ACTTCCAGTA TTGTGAACAA 
GCCAAATCAG TAATTGTGAG AAATACCAGT AGACAGACAA AGCCACTCAA AGTTCAAGTT 
ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTGTC TTGGCTGGGA 
AGTATTATTG GATATTCAGA TGGCCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GTGAAAACTC TTCTCTTGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTCATGA GCAGTCTTCT TATGGATTTG 
AAATACAAGA AGCTGTTTGC TGTTCGATTT GCAAAGAACT ATGAGCGTTT GCAGAGTGAT 
TATGTGACAG ATGACCACGA CAGAGAGTTT TCAGTCGCAG ACCTCTCGGT TCAGATATTC 
ACAGTTCCTT CACTTGCTCG GATGCTCATC ACTGAAGAAA ACCTGATGAC CATTATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA ATTTGAAAGA 
TATACTGCTT TACAAGCTTT CAAATTTAGG AGAGTTCAGA GCCTTATTTT AGACCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTGGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAGGTGGGAC AGCATATTGA AATGGAACCA GAGTGGGAAG CAGCCTTCAC GTTGCAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GATTGGTGTG CTTTAGATGA AAAAGTTTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCAGTA TTGATGCAGT GTCATGGCGG TTTTACTGAT 
GGTGAACAGC CAATCATATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCTCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTGCAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTCAGTGAA 
CTTAGCCCAC CTATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTT 
CATGCAGGAA TGTGGAGAAG AAATGGTTTC TCTCTGGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAA TAATGCTTCA GGTGTCTCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006926613.1
CDS	368..2470
Translation	MASELEPEVPAIDRSLLACSAEETAGKWLQATDLTREVYQHLAYYVPKIYCRGPNPLQEEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHKDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHISDVIARTYNIFAIMFRYAVEILTWEKENELPADLEMVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGSIIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAVRFAKNYERLQSDYVTDDHDREFSVADLSVQIFTVPSLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPIILSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIIMLQVSP

Target ORF information:

RefSeq Version	XM_006926551.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006926551.2

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC GGAGGTGCCG GCCATCGACC GGAGTTTGCT GGCATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCT ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT ACAGGAGGAA 
GACATGCTGG CACAGCATGT TTTGTTGGGA CCAATGGAAT GGTACCTTTG TGGTGAAGAT 
CCTGCATTTG GATTTCCAAA ACTTGAACAA GCAAACAAAC CTTCTCATCT TTGTGGTCGT 
GTTTTTAAAG TAGGAGAACC CACATATTCT TGCAGAGACT GTGCAGTTGA TCCAACTTGT 
GTTTTATGCA TGGAGTGCTT TTTGGGAAGT ATTCACAAAG ACCATCGATA TAGGATGACA 
ACATCAGGAG GTGGAGGTTT CTGTGACTGT GGTGATACTG AAGCTTGGAA AGAGGGTCCT 
TACTGTCAAA AACATGAACT TAACACCTCT GAAATTGAGG AAGAAGAGGA TCCTCTTGTG 
CATATATCAG ATGTGATAGC AAGAACTTAC AACATTTTTG CTATTATGTT TCGGTATGCA 
GTGGAGATAC TAACCTGGGA AAAAGAAAAT GAATTGCCAG CAGATTTAGA GATGGTAGAG 
AAGAGTGACA CCTACTATTG CATGCTTTTT AATGATGAGG TTCATACCTA TGAACAAGTT 
ATTTATACTC TTCAGAAAGC TGTTAACTGT ACACAAAAAG AAGCCATTGG CTTTGCAACT 
ACAGTAGATC GAGACGGACG TAGGTCTGTT CGATATGGAG ACTTCCAGTA TTGTGAACAA 
GCCAAATCAG TAATTGTGAG AAATACCAGT AGACAGACAA AGCCACTCAA AGTTCAAGTT 
ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTGTC TTGGCTGGGA 
AGTATTATTG GATATTCAGA TGGCCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GTGAAAACTC TTCTCTTGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTCATGA GCAGTCTTCT TATGGATTTG 
AAATACAAGA AGCTGTTTGC TGTTCGATTT GCAAAGAACT ATGAGCGTTT GCAGAGTGAT 
TATGTGACAG ATGACCACGA CAGAGAGTTT TCAGTCGCAG ACCTCTCGGT TCAGATATTC 
ACAGTTCCTT CACTTGCTCG GATGCTCATC ACTGAAGAAA ACCTGATGAC CATTATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA ATTTGAAAGA 
TATACTGCTT TACAAGCTTT CAAATTTAGG AGAGTTCAGA GCCTTATTTT AGACCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTGGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAGGTGGGAC AGCATATTGA AATGGAACCA GAGTGGGAAG CAGCCTTCAC GTTGCAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GATTGGTGTG CTTTAGATGA AAAAGTTTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCAGTA TTGATGCAGT GTCATGGCGG TTTTACTGAT 
GGTGAACAGC CAATCATATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCTCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTGCAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTCAGTGAA 
CTTAGCCCAC CTATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTT 
CATGCAGGAA TGTGGAGAAG AAATGGTTTC TCTCTGGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAA TAATGCTTCA GGTGTCTCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_015600428.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_015455914.1 Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPt31994
Clone ID Related Accession (Same CDS sequence)	XM_015600428.1
Accession Version	XM_015600428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	E3 ubiquitin-protein ligase UBR2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC GGAGGTGCCG GCCATCGACC GGAGTTTGCT GGCATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCT ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT ACAGGAGGAA 
GACATGCTGG CACAGCATGT TTTGTTGGGA CCAATGGAAT GGTACCTTTG TGGTGAAGAT 
CCTGCATTTG GATTTCCAAA ACTTGAACAA GCAAACAAAC CTTCTCATCT TTGTGGTCGT 
GTTTTTAAAG TAGGAGAACC CACATATTCT TGCAGAGACT GTGCAGTTGA TCCAACTTGT 
GTTTTATGCA TGGAGTGCTT TTTGGGAAGT ATTCACAAAG ACCATCGATA TAGGATGACA 
ACATCAGGAG GTGGAGGTTT CTGTGACTGT GGTGATACTG AAGCTTGGAA AGAGGGTCCT 
TACTGTCAAA AACATGAACT TAACACCTCT GAAATTGAGG AAGAAGAGGA TCCTCTTGTG 
CATATATCAG ATGTGATAGC AAGAACTTAC AACATTTTTG CTATTATGTT TCGGTATGCA 
GTGGAGATAC TAACCTGGGA AAAAGAAAAT GAATTGCCAG CAGATTTAGA GATGGTAGAG 
AAGAGTGACA CCTACTATTG CATGCTTTTT AATGATGAGG TTCATACCTA TGAACAAGTT 
ATTTATACTC TTCAGAAAGC TGTTAACTGT ACACAAAAAG AAGCCATTGG CTTTGCAACT 
ACAGTAGATC GAGACGGACG TAGGTCTGTT CGATATGGAG ACTTCCAGTA TTGTGAACAA 
GCCAAATCAG TAATTGTGAG AAATACCAGT AGACAGACAA AGCCACTCAA AGTTCAAGTT 
ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTGTC TTGGCTGGGA 
AGTATTATTG GATATTCAGA TGGCCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GTGAAAACTC TTCTCTTGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTCATGA GCAGTCTTCT TATGGATTTG 
AAATACAAGA AGCTGTTTGC TGTTCGATTT GCAAAGAACT ATGAGCGTTT GCAGAGTGAT 
TATGTGACAG ATGACCACGA CAGAGAGTTT TCAGTCGCAG ACCTCTCGGT TCAGATATTC 
ACAGTTCCTT CACTTGCTCG GATGCTCATC ACTGAAGAAA ACCTGATGAC CATTATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA ATTTGAAAGA 
TATACTGCTT TACAAGCTTT CAAATTTAGG AGAGTTCAGA GCCTTATTTT AGACCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTGGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAGGTGGGAC AGCATATTGA AATGGAACCA GAGTGGGAAG CAGCCTTCAC GTTGCAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GATTGGTGTG CTTTAGATGA AAAAGTTTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCAGTA TTGATGCAGT GTCATGGCGG TTTTACTGAT 
GGTGAACAGC CAATCATATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCTCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTGCAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTCAGTGAA 
CTTAGCCCAC CTATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTT 
CATGCAGGAA TGTGGAGAAG AAATGGTTTC TCTCTGGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAA TAATGCTTCA GACAGGTGTC 
TCCATGATGG ACCCAAATCA TTTCCTGATG ATAATGCTCA GCCGCTTTGA ACTTTATCAG 
ATTTTCAGTA CTCCAGACTA CGGAAAAAGA TTTAGTTCTG AGATTACCCA GAAGGATGTA 
GTTCAGCAAA ACAATACTTT AATAGAAGAA ATGTTATACC TCATTATAAT GCTTGTTGGA 
GAGAGATTTA ATCCTGGAGT TGGACAGGTA AATGCTACAG ATGAAATTAA GCGAGAGATT 
ATCCATCAGT TGAGCATCAA ACCTATGGCT CATAGTGAAT TGGTAAAATC TTTACCTGAA 
GATGAGAACA AGGAGACTGG CATGGAGAGT GTCATTGAAG AAGTTGCCCA TTTCAAGAAA 
CCTGGATTAA CAGGACGAGG CATGTATGAA CTGAAACCAG AGTGTGCCAA AGAGTTCAAT 
TTGTATTTCT ATCACTTTTC AAGGGCAGAG CAGTCCAAGG CAGAAGAAGC TCAACGGAAA 
TTGAAAAGAC AAAATAAGGA AGATACAGCA CTTCCACCTC CAGTTTTGCC TCCATTCTGC 
CCTCTGTTCG CAAGTCTGGT TAACATTTTG CAGTCAGATG TCATGTTGTG CATCATGAGA 
ACAATACTGC AGTGGGCTGT GGAACATAAT GGATACACCT GGTCTGAGTC CATGCTGCAA 
AGGGTATTAC ATTTAATTGG AATGGCACTA CAAGAAGAAA AACAGCACTT GGAGAATGTC 
ATGGAAGAGC ATATAGTAAC ATTTACCTTC ACTCAGAAGA TCTCAAAACC TGGTGAAGCA 
CCAAACAACT GTCCTAGTAT ACTAGCTATG CTGGAAACAC TGCAAAATGC TCCCTACCTA 
GAAGTCCACA AAGATATGAT TAGATGGATA TTAAAGACTT TTAATACTAT TAAGAAAATG 
AGGGAGAGTT CATCTGTCAG TCCTGTAGCA GAGACAGAAG GAACCATAAT GGAAGAGAGT 
TCAAGAGACA AAGACAAAGC TGAGAGGAAG AGAAAAGCTG AGATTGCCAG ACTGCGCAGA 
GAAAAGATCA TGGCCCAGAT GTCTGAGATG CAGCGGCACT TCATTGATGA GAACAAAGAA 
CTCTTCCAGC AGACATTAGA ACTGGATGTC TCCACCTCTG CTGCTCTTGA TAATAGCCCT 
ATGATTTCAG ATATGACATT TACAGCATTG GGCCCAGCAC AAACTCAGGT CCCTAAACAA 
AGACAATTTG TTACCTGTAT ATTGTGTCAA GAGGAACAAG AGGTTAAGGT GGAAAGCAGG 
GCAATGGTCC TGGCAGCATT TGTTCAAAGA TCAACTGTAT TATCAAAAAA CAGAAGTAAA 
TTCATTCAGG ATCCAGAAAA ATATGATCCA TTATTCATGC ACCCTAATCT GTCTTGTGGA 
ACACACACCA GTAGCTGTGG ACACATTATG CATGCCCATT GTTGGCAAAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015455914.1
CDS	367..3900
Translation	MASELEPEVPAIDRSLLACSAEETAGKWLQATDLTREVYQHLAYYVPKIYCRGPNPLQEEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHKDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHISDVIARTYNIFAIMFRYAVEILTWEKENELPADLEMVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGSIIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAVRFAKNYERLQSDYVTDDHDREFSVADLSVQIFTVPSLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPIILSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIIMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITQKDVVQQNNTLIEEMLYLIIMLVGERFNPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEEVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNKEDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTILQWAVEHNGYTWSESMLQRVLHLIGMALQEEKQHLENVMEEHIVTFTFTQKISKPGEAPNNCPSILAMLETLQNAPYLEVHKDMIRWILKTFNTIKKMRESSSVSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDVSTSAALDNSPMISDMTFTALGPAQTQVPKQRQFVTCILCQEEQEVKVESRAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPNLSCGTHTSSCGHIMHAHCWQS

Target ORF information:

RefSeq Version	XM_015600428.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015600428.1

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC GGAGGTGCCG GCCATCGACC GGAGTTTGCT GGCATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCT ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT ACAGGAGGAA 
GACATGCTGG CACAGCATGT TTTGTTGGGA CCAATGGAAT GGTACCTTTG TGGTGAAGAT 
CCTGCATTTG GATTTCCAAA ACTTGAACAA GCAAACAAAC CTTCTCATCT TTGTGGTCGT 
GTTTTTAAAG TAGGAGAACC CACATATTCT TGCAGAGACT GTGCAGTTGA TCCAACTTGT 
GTTTTATGCA TGGAGTGCTT TTTGGGAAGT ATTCACAAAG ACCATCGATA TAGGATGACA 
ACATCAGGAG GTGGAGGTTT CTGTGACTGT GGTGATACTG AAGCTTGGAA AGAGGGTCCT 
TACTGTCAAA AACATGAACT TAACACCTCT GAAATTGAGG AAGAAGAGGA TCCTCTTGTG 
CATATATCAG ATGTGATAGC AAGAACTTAC AACATTTTTG CTATTATGTT TCGGTATGCA 
GTGGAGATAC TAACCTGGGA AAAAGAAAAT GAATTGCCAG CAGATTTAGA GATGGTAGAG 
AAGAGTGACA CCTACTATTG CATGCTTTTT AATGATGAGG TTCATACCTA TGAACAAGTT 
ATTTATACTC TTCAGAAAGC TGTTAACTGT ACACAAAAAG AAGCCATTGG CTTTGCAACT 
ACAGTAGATC GAGACGGACG TAGGTCTGTT CGATATGGAG ACTTCCAGTA TTGTGAACAA 
GCCAAATCAG TAATTGTGAG AAATACCAGT AGACAGACAA AGCCACTCAA AGTTCAAGTT 
ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTGTC TTGGCTGGGA 
AGTATTATTG GATATTCAGA TGGCCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GTGAAAACTC TTCTCTTGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTCATGA GCAGTCTTCT TATGGATTTG 
AAATACAAGA AGCTGTTTGC TGTTCGATTT GCAAAGAACT ATGAGCGTTT GCAGAGTGAT 
TATGTGACAG ATGACCACGA CAGAGAGTTT TCAGTCGCAG ACCTCTCGGT TCAGATATTC 
ACAGTTCCTT CACTTGCTCG GATGCTCATC ACTGAAGAAA ACCTGATGAC CATTATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA ATTTGAAAGA 
TATACTGCTT TACAAGCTTT CAAATTTAGG AGAGTTCAGA GCCTTATTTT AGACCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTGGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAGGTGGGAC AGCATATTGA AATGGAACCA GAGTGGGAAG CAGCCTTCAC GTTGCAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GATTGGTGTG CTTTAGATGA AAAAGTTTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCAGTA TTGATGCAGT GTCATGGCGG TTTTACTGAT 
GGTGAACAGC CAATCATATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCTCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTGCAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTCAGTGAA 
CTTAGCCCAC CTATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTT 
CATGCAGGAA TGTGGAGAAG AAATGGTTTC TCTCTGGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAA TAATGCTTCA GACAGGTGTC 
TCCATGATGG ACCCAAATCA TTTCCTGATG ATAATGCTCA GCCGCTTTGA ACTTTATCAG 
ATTTTCAGTA CTCCAGACTA CGGAAAAAGA TTTAGTTCTG AGATTACCCA GAAGGATGTA 
GTTCAGCAAA ACAATACTTT AATAGAAGAA ATGTTATACC TCATTATAAT GCTTGTTGGA 
GAGAGATTTA ATCCTGGAGT TGGACAGGTA AATGCTACAG ATGAAATTAA GCGAGAGATT 
ATCCATCAGT TGAGCATCAA ACCTATGGCT CATAGTGAAT TGGTAAAATC TTTACCTGAA 
GATGAGAACA AGGAGACTGG CATGGAGAGT GTCATTGAAG AAGTTGCCCA TTTCAAGAAA 
CCTGGATTAA CAGGACGAGG CATGTATGAA CTGAAACCAG AGTGTGCCAA AGAGTTCAAT 
TTGTATTTCT ATCACTTTTC AAGGGCAGAG CAGTCCAAGG CAGAAGAAGC TCAACGGAAA 
TTGAAAAGAC AAAATAAGGA AGATACAGCA CTTCCACCTC CAGTTTTGCC TCCATTCTGC 
CCTCTGTTCG CAAGTCTGGT TAACATTTTG CAGTCAGATG TCATGTTGTG CATCATGAGA 
ACAATACTGC AGTGGGCTGT GGAACATAAT GGATACACCT GGTCTGAGTC CATGCTGCAA 
AGGGTATTAC ATTTAATTGG AATGGCACTA CAAGAAGAAA AACAGCACTT GGAGAATGTC 
ATGGAAGAGC ATATAGTAAC ATTTACCTTC ACTCAGAAGA TCTCAAAACC TGGTGAAGCA 
CCAAACAACT GTCCTAGTAT ACTAGCTATG CTGGAAACAC TGCAAAATGC TCCCTACCTA 
GAAGTCCACA AAGATATGAT TAGATGGATA TTAAAGACTT TTAATACTAT TAAGAAAATG 
AGGGAGAGTT CATCTGTCAG TCCTGTAGCA GAGACAGAAG GAACCATAAT GGAAGAGAGT 
TCAAGAGACA AAGACAAAGC TGAGAGGAAG AGAAAAGCTG AGATTGCCAG ACTGCGCAGA 
GAAAAGATCA TGGCCCAGAT GTCTGAGATG CAGCGGCACT TCATTGATGA GAACAAAGAA 
CTCTTCCAGC AGACATTAGA ACTGGATGTC TCCACCTCTG CTGCTCTTGA TAATAGCCCT 
ATGATTTCAG ATATGACATT TACAGCATTG GGCCCAGCAC AAACTCAGGT CCCTAAACAA 
AGACAATTTG TTACCTGTAT ATTGTGTCAA GAGGAACAAG AGGTTAAGGT GGAAAGCAGG 
GCAATGGTCC TGGCAGCATT TGTTCAAAGA TCAACTGTAT TATCAAAAAA CAGAAGTAAA 
TTCATTCAGG ATCCAGAAAA ATATGATCCA TTATTCATGC ACCCTAATCT GTCTTGTGGA 
ACACACACCA GTAGCTGTGG ACACATTATG CATGCCCATT GTTGGCAAAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_006926549.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006926611.1 Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.

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CloneID	OPt31991
Clone ID Related Accession (Same CDS sequence)	XM_006926549.2 , XM_006926549.3
Accession Version	XM_006926549.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	E3 ubiquitin-protein ligase UBR2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 10, 2016 this sequence version replaced XM_006926549.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC GGAGGTGCCG GCCATCGACC GGAGTTTGCT GGCATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCT ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT ACAGGAGGAA 
GACATGCTGG CACAGCATGT TTTGTTGGGA CCAATGGAAT GGTACCTTTG TGGTGAAGAT 
CCTGCATTTG GATTTCCAAA ACTTGAACAA GCAAACAAAC CTTCTCATCT TTGTGGTCGT 
GTTTTTAAAG TAGGAGAACC CACATATTCT TGCAGAGACT GTGCAGTTGA TCCAACTTGT 
GTTTTATGCA TGGAGTGCTT TTTGGGAAGT ATTCACAAAG ACCATCGATA TAGGATGACA 
ACATCAGGAG GTGGAGGTTT CTGTGACTGT GGTGATACTG AAGCTTGGAA AGAGGGTCCT 
TACTGTCAAA AACATGAACT TAACACCTCT GAAATTGAGG AAGAAGAGGA TCCTCTTGTG 
CATATATCAG ATGTGATAGC AAGAACTTAC AACATTTTTG CTATTATGTT TCGGTATGCA 
GTGGAGATAC TAACCTGGGA AAAAGAAAAT GAATTGCCAG CAGATTTAGA GATGGTAGAG 
AAGAGTGACA CCTACTATTG CATGCTTTTT AATGATGAGG TTCATACCTA TGAACAAGTT 
ATTTATACTC TTCAGAAAGC TGTTAACTGT ACACAAAAAG AAGCCATTGG CTTTGCAACT 
ACAGTAGATC GAGACGGACG TAGGTCTGTT CGATATGGAG ACTTCCAGTA TTGTGAACAA 
GCCAAATCAG TAATTGTGAG AAATACCAGT AGACAGACAA AGCCACTCAA AGTTCAAGTT 
ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTGTC TTGGCTGGGA 
AGTATTATTG GATATTCAGA TGGCCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GTGAAAACTC TTCTCTTGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTCATGA GCAGTCTTCT TATGGATTTG 
AAATACAAGA AGCTGTTTGC TGTTCGATTT GCAAAGAATT ACCAGCAGTT GCAGAGAGAT 
TTTATGGAGG ATGATCACGA GCGAGCAGTG TCCGTGACTG CTCTGTCTGT CCAGTTCTTC 
ACTGCACCTA CTCTGGCTCG GATGCTCATC ACTGAAGAAA ACCTGATGAC CATTATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA ATTTGAAAGA 
TATACTGCTT TACAAGCTTT CAAATTTAGG AGAGTTCAGA GCCTTATTTT AGACCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTGGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAGGTGGGAC AGCATATTGA AATGGAACCA GAGTGGGAAG CAGCCTTCAC GTTGCAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GATTGGTGTG CTTTAGATGA AAAAGTTTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCAGTA TTGATGCAGT GTCATGGCGG TTTTACTGAT 
GGTGAACAGC CAATCATATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCTCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTGCAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTCAGTGAA 
CTTAGCCCAC CTATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTT 
CATGCAGGAA TGTGGAGAAG AAATGGTTTC TCTCTGGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAA TAATGCTTCA GACAGGTGTC 
TCCATGATGG ACCCAAATCA TTTCCTGATG ATAATGCTCA GCCGCTTTGA ACTTTATCAG 
ATTTTCAGTA CTCCAGACTA CGGAAAAAGA TTTAGTTCTG AGATTACCCA GAAGGATGTA 
GTTCAGCAAA ACAATACTTT AATAGAAGAA ATGTTATACC TCATTATAAT GCTTGTTGGA 
GAGAGATTTA ATCCTGGAGT TGGACAGGTA AATGCTACAG ATGAAATTAA GCGAGAGATT 
ATCCATCAGT TGAGCATCAA ACCTATGGCT CATAGTGAAT TGGTAAAATC TTTACCTGAA 
GATGAGAACA AGGAGACTGG CATGGAGAGT GTCATTGAAG AAGTTGCCCA TTTCAAGAAA 
CCTGGATTAA CAGGACGAGG CATGTATGAA CTGAAACCAG AGTGTGCCAA AGAGTTCAAT 
TTGTATTTCT ATCACTTTTC AAGGGCAGAG CAGTCCAAGG CAGAAGAAGC TCAACGGAAA 
TTGAAAAGAC AAAATAAGGA AGATACAGCA CTTCCACCTC CAGTTTTGCC TCCATTCTGC 
CCTCTGTTCG CAAGTCTGGT TAACATTTTG CAGTCAGATG TCATGTTGTG CATCATGAGA 
ACAATACTGC AGTGGGCTGT GGAACATAAT GGATACACCT GGTCTGAGTC CATGCTGCAA 
AGGGTATTAC ATTTAATTGG AATGGCACTA CAAGAAGAAA AACAGCACTT GGAGAATGTC 
ATGGAAGAGC ATATAGTAAC ATTTACCTTC ACTCAGAAGA TCTCAAAACC TGGTGAAGCA 
CCAAACAACT GTCCTAGTAT ACTAGCTATG CTGGAAACAC TGCAAAATGC TCCCTACCTA 
GAAGTCCACA AAGATATGAT TAGATGGATA TTAAAGACTT TTAATACTAT TAAGAAAATG 
AGGGAGAGTT CATCTGTCAG TCCTGTAGCA GAGACAGAAG GAACCATAAT GGAAGAGAGT 
TCAAGAGACA AAGACAAAGC TGAGAGGAAG AGAAAAGCTG AGATTGCCAG ACTGCGCAGA 
GAAAAGATCA TGGCCCAGAT GTCTGAGATG CAGCGGCACT TCATTGATGA GAACAAAGAA 
CTCTTCCAGC AGACATTAGA ACTGGATGTC TCCACCTCTG CTGCTCTTGA TAATAGCCCT 
ATGATTTCAG ATATGACATT TACAGCATTG GGCCCAGCAC AAACTCAGGT CCCTAAACAA 
AGACAATTTG TTACCTGTAT ATTGTGTCAA GAGGAACAAG AGGTTAAGGT GGAAAGCAGG 
GCAATGGTCC TGGCAGCATT TGTTCAAAGA TCAACTGTAT TATCAAAAAA CAGAAGTAAA 
TTCATTCAGG ATCCAGAAAA ATATGATCCA TTATTCATGC ACCCTAATCT GTCTTGTGGA 
ACACACACCA GTAGCTGTGG ACACATTATG CATGCCCATT GTTGGCAAAG GTATTTTGAT 
TCCGTTCAAG CTAAAGAACA GCGAAGGCAA CAGAGATTAC GCTTACATAC AAGCTATGAC 
GTAGAAAATG GAGAATTCCT GTGTCCCCTT TGTGAATGTT TGAGTAACAC TGTTATTCCT 
CTGCTGTTTC CTCCAAGAAA TATTTCTAAC AACAGGTTAA ATTTTTCAGA CCAACCAAAT 
CTGACTCAGT GGATTAGAAC AATATCTCAG CAAATAAAAG CATTGCAGAT TCTTAGGAAA 
GAAGAAAGTA TTCCTAATAT TGTCTCTTCA AAGAATTCAG AAAATATGGA TGAATTGCAG 
CTCCCTGAAG GATTTAGGCC TGATTTTCAT CCTAAGAACC CTTATTCTGA TAGCATAAAA 
GAAATGCTAA CAACGTTTGG AACTGCTACT TACAAGGTGG GACTAAAGGT TCATCCCAAT 
GAAGAAGATC CCCGCGTGCC CATAATGTGT TGGGGTAGTT GTGCGTACAC CATCCAAAGC 
ATAGAAAGAA TCTTAAGTGA TGAAGATAAA CCGTTGTTTG GTCCTTTGCC TTGCAGACTG 
GATGACTGTC TTAGGTCACT AACAAGGTTC GCTGCAGCAC ACTGGACAGT GGCATTACTC 
TCAGTGGTGC AGGAACATTT TTGTAGACTT TTTGCATCAT TGGTGCCTGA TGACAATCAT 
GAAGACCTTC CATGCATATT AGATATCGAC ATGTTTCATT TATTGGTGGG CTTGGTGCTC 
GCTTTCCCCG CGTTGCAGTG TCAGGATTTT TCAGGGATCA GCCTTGAAAC TGGAGACCTT 
CACATTTTCC ATCTGGTTAC TATGGCACAC ATTGTACAGA TCTTACTTAC CTCGTGTACA 
GAAGAGAATG GCATGGATCA AGAAACTCCT GCTGGTGAAG AAGAATTAGC AGTCCTTGCT 
TTGTATAAAA CACTTCGCCA GTACACAGGA AGTGCCTTGA AAGACATACC CTCTGGCTGG 
CATCTGTGGA GGAATGTCAG AGCTGGAATC ATGCCTTTCC TAAAGTGTTC TGCTTTGTTT 
TTTCATTACT TAAATGGAGT TCCTTCTCCA CCTGAAATTC AAGTTTCTGG AACAAGCCAT 
TTTGAACATT TATGTAACTA TCTTTCCCTG CCAAACAACC TCATTTGTCT TTTTCAAGAA 
AATGATGAGA TAATGAAATT ACTGATTGAA AGTTGGTGTC AGAACACTGA AGTTAAAAGA 
TATCTAGAAG GTAAAAGAGA TGCTATCAGC TATCCAAGAG AATCTAACAA ATTAATAGAC 
CTTCCAGAGG ATTACAGCAG CCTCATTAAT CAAGCATCTA ATTTCTCGTG CCCCAAATCA 
GGTGGTGACA AGAGCAGAGC CCCAACTCTG TGCCTTGTGT GTGGAACTCT GCTGTGCTCC 
CAGAGCTACT GCTGTCAGAC CGAACTGGAA GGGGAGGATG TAGGAGCTTG CACAGCTCAC 
ACCTACTCCT GTGGTTCTGG GGTGGGCATC TTCCTGAGAG TACGAGAATG TCAGGTGCTA 
TTTTTAGCCG GCAAAACCAA AGGCTGTTTT TATTCTCCTC CTTACCTTGA TGACTATGGG 
GAGACCGACC AGGGACTCAG ACGGGGAAAT CCTTTACATC TGTGCAAAGA GCGATTTAAG 
AAGATTCAGA AGCTCTGGCA CCAGCACAGT ATCACAGAGG AAATTGGACA TGCGCAGGAA 
GCAAATCAGA CGCTGGTTGG CATTGACTGG CAACATTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006926611.1
CDS	365..5626
Translation	MASELEPEVPAIDRSLLACSAEETAGKWLQATDLTREVYQHLAYYVPKIYCRGPNPLQEEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHKDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHISDVIARTYNIFAIMFRYAVEILTWEKENELPADLEMVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGSIIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAVRFAKNYQQLQRDFMEDDHERAVSVTALSVQFFTAPTLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPIILSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIIMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITQKDVVQQNNTLIEEMLYLIIMLVGERFNPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEEVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNKEDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTILQWAVEHNGYTWSESMLQRVLHLIGMALQEEKQHLENVMEEHIVTFTFTQKISKPGEAPNNCPSILAMLETLQNAPYLEVHKDMIRWILKTFNTIKKMRESSSVSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDVSTSAALDNSPMISDMTFTALGPAQTQVPKQRQFVTCILCQEEQEVKVESRAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPNLSCGTHTSSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLFPPRNISNNRLNFSDQPNLTQWIRTISQQIKALQILRKEESIPNIVSSKNSENMDELQLPEGFRPDFHPKNPYSDSIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVALLSVVQEHFCRLFASLVPDDNHEDLPCILDIDMFHLLVGLVLAFPALQCQDFSGISLETGDLHIFHLVTMAHIVQILLTSCTEENGMDQETPAGEEELAVLALYKTLRQYTGSALKDIPSGWHLWRNVRAGIMPFLKCSALFFHYLNGVPSPPEIQVSGTSHFEHLCNYLSLPNNLICLFQENDEIMKLLIESWCQNTEVKRYLEGKRDAISYPRESNKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGTLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_006926549.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006926549.2

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC GGAGGTGCCG GCCATCGACC GGAGTTTGCT GGCATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCT ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT ACAGGAGGAA 
GACATGCTGG CACAGCATGT TTTGTTGGGA CCAATGGAAT GGTACCTTTG TGGTGAAGAT 
CCTGCATTTG GATTTCCAAA ACTTGAACAA GCAAACAAAC CTTCTCATCT TTGTGGTCGT 
GTTTTTAAAG TAGGAGAACC CACATATTCT TGCAGAGACT GTGCAGTTGA TCCAACTTGT 
GTTTTATGCA TGGAGTGCTT TTTGGGAAGT ATTCACAAAG ACCATCGATA TAGGATGACA 
ACATCAGGAG GTGGAGGTTT CTGTGACTGT GGTGATACTG AAGCTTGGAA AGAGGGTCCT 
TACTGTCAAA AACATGAACT TAACACCTCT GAAATTGAGG AAGAAGAGGA TCCTCTTGTG 
CATATATCAG ATGTGATAGC AAGAACTTAC AACATTTTTG CTATTATGTT TCGGTATGCA 
GTGGAGATAC TAACCTGGGA AAAAGAAAAT GAATTGCCAG CAGATTTAGA GATGGTAGAG 
AAGAGTGACA CCTACTATTG CATGCTTTTT AATGATGAGG TTCATACCTA TGAACAAGTT 
ATTTATACTC TTCAGAAAGC TGTTAACTGT ACACAAAAAG AAGCCATTGG CTTTGCAACT 
ACAGTAGATC GAGACGGACG TAGGTCTGTT CGATATGGAG ACTTCCAGTA TTGTGAACAA 
GCCAAATCAG TAATTGTGAG AAATACCAGT AGACAGACAA AGCCACTCAA AGTTCAAGTT 
ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTGTC TTGGCTGGGA 
AGTATTATTG GATATTCAGA TGGCCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GTGAAAACTC TTCTCTTGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTCATGA GCAGTCTTCT TATGGATTTG 
AAATACAAGA AGCTGTTTGC TGTTCGATTT GCAAAGAATT ACCAGCAGTT GCAGAGAGAT 
TTTATGGAGG ATGATCACGA GCGAGCAGTG TCCGTGACTG CTCTGTCTGT CCAGTTCTTC 
ACTGCACCTA CTCTGGCTCG GATGCTCATC ACTGAAGAAA ACCTGATGAC CATTATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA ATTTGAAAGA 
TATACTGCTT TACAAGCTTT CAAATTTAGG AGAGTTCAGA GCCTTATTTT AGACCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTGGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAGGTGGGAC AGCATATTGA AATGGAACCA GAGTGGGAAG CAGCCTTCAC GTTGCAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GATTGGTGTG CTTTAGATGA AAAAGTTTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCAGTA TTGATGCAGT GTCATGGCGG TTTTACTGAT 
GGTGAACAGC CAATCATATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCTCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTGCAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTCAGTGAA 
CTTAGCCCAC CTATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTT 
CATGCAGGAA TGTGGAGAAG AAATGGTTTC TCTCTGGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAA TAATGCTTCA GACAGGTGTC 
TCCATGATGG ACCCAAATCA TTTCCTGATG ATAATGCTCA GCCGCTTTGA ACTTTATCAG 
ATTTTCAGTA CTCCAGACTA CGGAAAAAGA TTTAGTTCTG AGATTACCCA GAAGGATGTA 
GTTCAGCAAA ACAATACTTT AATAGAAGAA ATGTTATACC TCATTATAAT GCTTGTTGGA 
GAGAGATTTA ATCCTGGAGT TGGACAGGTA AATGCTACAG ATGAAATTAA GCGAGAGATT 
ATCCATCAGT TGAGCATCAA ACCTATGGCT CATAGTGAAT TGGTAAAATC TTTACCTGAA 
GATGAGAACA AGGAGACTGG CATGGAGAGT GTCATTGAAG AAGTTGCCCA TTTCAAGAAA 
CCTGGATTAA CAGGACGAGG CATGTATGAA CTGAAACCAG AGTGTGCCAA AGAGTTCAAT 
TTGTATTTCT ATCACTTTTC AAGGGCAGAG CAGTCCAAGG CAGAAGAAGC TCAACGGAAA 
TTGAAAAGAC AAAATAAGGA AGATACAGCA CTTCCACCTC CAGTTTTGCC TCCATTCTGC 
CCTCTGTTCG CAAGTCTGGT TAACATTTTG CAGTCAGATG TCATGTTGTG CATCATGAGA 
ACAATACTGC AGTGGGCTGT GGAACATAAT GGATACACCT GGTCTGAGTC CATGCTGCAA 
AGGGTATTAC ATTTAATTGG AATGGCACTA CAAGAAGAAA AACAGCACTT GGAGAATGTC 
ATGGAAGAGC ATATAGTAAC ATTTACCTTC ACTCAGAAGA TCTCAAAACC TGGTGAAGCA 
CCAAACAACT GTCCTAGTAT ACTAGCTATG CTGGAAACAC TGCAAAATGC TCCCTACCTA 
GAAGTCCACA AAGATATGAT TAGATGGATA TTAAAGACTT TTAATACTAT TAAGAAAATG 
AGGGAGAGTT CATCTGTCAG TCCTGTAGCA GAGACAGAAG GAACCATAAT GGAAGAGAGT 
TCAAGAGACA AAGACAAAGC TGAGAGGAAG AGAAAAGCTG AGATTGCCAG ACTGCGCAGA 
GAAAAGATCA TGGCCCAGAT GTCTGAGATG CAGCGGCACT TCATTGATGA GAACAAAGAA 
CTCTTCCAGC AGACATTAGA ACTGGATGTC TCCACCTCTG CTGCTCTTGA TAATAGCCCT 
ATGATTTCAG ATATGACATT TACAGCATTG GGCCCAGCAC AAACTCAGGT CCCTAAACAA 
AGACAATTTG TTACCTGTAT ATTGTGTCAA GAGGAACAAG AGGTTAAGGT GGAAAGCAGG 
GCAATGGTCC TGGCAGCATT TGTTCAAAGA TCAACTGTAT TATCAAAAAA CAGAAGTAAA 
TTCATTCAGG ATCCAGAAAA ATATGATCCA TTATTCATGC ACCCTAATCT GTCTTGTGGA 
ACACACACCA GTAGCTGTGG ACACATTATG CATGCCCATT GTTGGCAAAG GTATTTTGAT 
TCCGTTCAAG CTAAAGAACA GCGAAGGCAA CAGAGATTAC GCTTACATAC AAGCTATGAC 
GTAGAAAATG GAGAATTCCT GTGTCCCCTT TGTGAATGTT TGAGTAACAC TGTTATTCCT 
CTGCTGTTTC CTCCAAGAAA TATTTCTAAC AACAGGTTAA ATTTTTCAGA CCAACCAAAT 
CTGACTCAGT GGATTAGAAC AATATCTCAG CAAATAAAAG CATTGCAGAT TCTTAGGAAA 
GAAGAAAGTA TTCCTAATAT TGTCTCTTCA AAGAATTCAG AAAATATGGA TGAATTGCAG 
CTCCCTGAAG GATTTAGGCC TGATTTTCAT CCTAAGAACC CTTATTCTGA TAGCATAAAA 
GAAATGCTAA CAACGTTTGG AACTGCTACT TACAAGGTGG GACTAAAGGT TCATCCCAAT 
GAAGAAGATC CCCGCGTGCC CATAATGTGT TGGGGTAGTT GTGCGTACAC CATCCAAAGC 
ATAGAAAGAA TCTTAAGTGA TGAAGATAAA CCGTTGTTTG GTCCTTTGCC TTGCAGACTG 
GATGACTGTC TTAGGTCACT AACAAGGTTC GCTGCAGCAC ACTGGACAGT GGCATTACTC 
TCAGTGGTGC AGGAACATTT TTGTAGACTT TTTGCATCAT TGGTGCCTGA TGACAATCAT 
GAAGACCTTC CATGCATATT AGATATCGAC ATGTTTCATT TATTGGTGGG CTTGGTGCTC 
GCTTTCCCCG CGTTGCAGTG TCAGGATTTT TCAGGGATCA GCCTTGAAAC TGGAGACCTT 
CACATTTTCC ATCTGGTTAC TATGGCACAC ATTGTACAGA TCTTACTTAC CTCGTGTACA 
GAAGAGAATG GCATGGATCA AGAAACTCCT GCTGGTGAAG AAGAATTAGC AGTCCTTGCT 
TTGTATAAAA CACTTCGCCA GTACACAGGA AGTGCCTTGA AAGACATACC CTCTGGCTGG 
CATCTGTGGA GGAATGTCAG AGCTGGAATC ATGCCTTTCC TAAAGTGTTC TGCTTTGTTT 
TTTCATTACT TAAATGGAGT TCCTTCTCCA CCTGAAATTC AAGTTTCTGG AACAAGCCAT 
TTTGAACATT TATGTAACTA TCTTTCCCTG CCAAACAACC TCATTTGTCT TTTTCAAGAA 
AATGATGAGA TAATGAAATT ACTGATTGAA AGTTGGTGTC AGAACACTGA AGTTAAAAGA 
TATCTAGAAG GTAAAAGAGA TGCTATCAGC TATCCAAGAG AATCTAACAA ATTAATAGAC 
CTTCCAGAGG ATTACAGCAG CCTCATTAAT CAAGCATCTA ATTTCTCGTG CCCCAAATCA 
GGTGGTGACA AGAGCAGAGC CCCAACTCTG TGCCTTGTGT GTGGAACTCT GCTGTGCTCC 
CAGAGCTACT GCTGTCAGAC CGAACTGGAA GGGGAGGATG TAGGAGCTTG CACAGCTCAC 
ACCTACTCCT GTGGTTCTGG GGTGGGCATC TTCCTGAGAG TACGAGAATG TCAGGTGCTA 
TTTTTAGCCG GCAAAACCAA AGGCTGTTTT TATTCTCCTC CTTACCTTGA TGACTATGGG 
GAGACCGACC AGGGACTCAG ACGGGGAAAT CCTTTACATC TGTGCAAAGA GCGATTTAAG 
AAGATTCAGA AGCTCTGGCA CCAGCACAGT ATCACAGAGG AAATTGGACA TGCGCAGGAA 
GCAAATCAGA CGCTGGTTGG CATTGACTGG CAACATTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_006926549.3 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006926611.1 Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.

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CloneID	OPt31991
Clone ID Related Accession (Same CDS sequence)	XM_006926549.2 , XM_006926549.3
Accession Version	XM_006926549.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	E3 ubiquitin-protein ligase UBR2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_006926549.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC GGAGGTGCCG GCCATCGACC GGAGTTTGCT GGCATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCT ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT ACAGGAGGAA 
GACATGCTGG CACAGCATGT TTTGTTGGGA CCAATGGAAT GGTACCTTTG TGGTGAAGAT 
CCTGCATTTG GATTTCCAAA ACTTGAACAA GCAAACAAAC CTTCTCATCT TTGTGGTCGT 
GTTTTTAAAG TAGGAGAACC CACATATTCT TGCAGAGACT GTGCAGTTGA TCCAACTTGT 
GTTTTATGCA TGGAGTGCTT TTTGGGAAGT ATTCACAAAG ACCATCGATA TAGGATGACA 
ACATCAGGAG GTGGAGGTTT CTGTGACTGT GGTGATACTG AAGCTTGGAA AGAGGGTCCT 
TACTGTCAAA AACATGAACT TAACACCTCT GAAATTGAGG AAGAAGAGGA TCCTCTTGTG 
CATATATCAG ATGTGATAGC AAGAACTTAC AACATTTTTG CTATTATGTT TCGGTATGCA 
GTGGAGATAC TAACCTGGGA AAAAGAAAAT GAATTGCCAG CAGATTTAGA GATGGTAGAG 
AAGAGTGACA CCTACTATTG CATGCTTTTT AATGATGAGG TTCATACCTA TGAACAAGTT 
ATTTATACTC TTCAGAAAGC TGTTAACTGT ACACAAAAAG AAGCCATTGG CTTTGCAACT 
ACAGTAGATC GAGACGGACG TAGGTCTGTT CGATATGGAG ACTTCCAGTA TTGTGAACAA 
GCCAAATCAG TAATTGTGAG AAATACCAGT AGACAGACAA AGCCACTCAA AGTTCAAGTT 
ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTGTC TTGGCTGGGA 
AGTATTATTG GATATTCAGA TGGCCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GTGAAAACTC TTCTCTTGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTCATGA GCAGTCTTCT TATGGATTTG 
AAATACAAGA AGCTGTTTGC TGTTCGATTT GCAAAGAATT ACCAGCAGTT GCAGAGAGAT 
TTTATGGAGG ATGATCACGA GCGAGCAGTG TCCGTGACTG CTCTGTCTGT CCAGTTCTTC 
ACTGCACCTA CTCTGGCTCG GATGCTCATC ACTGAAGAAA ACCTGATGAC CATTATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA ATTTGAAAGA 
TATACTGCTT TACAAGCTTT CAAATTTAGG AGAGTTCAGA GCCTTATTTT AGACCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTGGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAGGTGGGAC AGCATATTGA AATGGAACCA GAGTGGGAAG CAGCCTTCAC GTTGCAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GATTGGTGTG CTTTAGATGA AAAAGTTTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCAGTA TTGATGCAGT GTCATGGCGG TTTTACTGAT 
GGTGAACAGC CAATCATATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCTCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTGCAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTCAGTGAA 
CTTAGCCCAC CTATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTT 
CATGCAGGAA TGTGGAGAAG AAATGGTTTC TCTCTGGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAA TAATGCTTCA GACAGGTGTC 
TCCATGATGG ACCCAAATCA TTTCCTGATG ATAATGCTCA GCCGCTTTGA ACTTTATCAG 
ATTTTCAGTA CTCCAGACTA CGGAAAAAGA TTTAGTTCTG AGATTACCCA GAAGGATGTA 
GTTCAGCAAA ACAATACTTT AATAGAAGAA ATGTTATACC TCATTATAAT GCTTGTTGGA 
GAGAGATTTA ATCCTGGAGT TGGACAGGTA AATGCTACAG ATGAAATTAA GCGAGAGATT 
ATCCATCAGT TGAGCATCAA ACCTATGGCT CATAGTGAAT TGGTAAAATC TTTACCTGAA 
GATGAGAACA AGGAGACTGG CATGGAGAGT GTCATTGAAG AAGTTGCCCA TTTCAAGAAA 
CCTGGATTAA CAGGACGAGG CATGTATGAA CTGAAACCAG AGTGTGCCAA AGAGTTCAAT 
TTGTATTTCT ATCACTTTTC AAGGGCAGAG CAGTCCAAGG CAGAAGAAGC TCAACGGAAA 
TTGAAAAGAC AAAATAAGGA AGATACAGCA CTTCCACCTC CAGTTTTGCC TCCATTCTGC 
CCTCTGTTCG CAAGTCTGGT TAACATTTTG CAGTCAGATG TCATGTTGTG CATCATGAGA 
ACAATACTGC AGTGGGCTGT GGAACATAAT GGATACACCT GGTCTGAGTC CATGCTGCAA 
AGGGTATTAC ATTTAATTGG AATGGCACTA CAAGAAGAAA AACAGCACTT GGAGAATGTC 
ATGGAAGAGC ATATAGTAAC ATTTACCTTC ACTCAGAAGA TCTCAAAACC TGGTGAAGCA 
CCAAACAACT GTCCTAGTAT ACTAGCTATG CTGGAAACAC TGCAAAATGC TCCCTACCTA 
GAAGTCCACA AAGATATGAT TAGATGGATA TTAAAGACTT TTAATACTAT TAAGAAAATG 
AGGGAGAGTT CATCTGTCAG TCCTGTAGCA GAGACAGAAG GAACCATAAT GGAAGAGAGT 
TCAAGAGACA AAGACAAAGC TGAGAGGAAG AGAAAAGCTG AGATTGCCAG ACTGCGCAGA 
GAAAAGATCA TGGCCCAGAT GTCTGAGATG CAGCGGCACT TCATTGATGA GAACAAAGAA 
CTCTTCCAGC AGACATTAGA ACTGGATGTC TCCACCTCTG CTGCTCTTGA TAATAGCCCT 
ATGATTTCAG ATATGACATT TACAGCATTG GGCCCAGCAC AAACTCAGGT CCCTAAACAA 
AGACAATTTG TTACCTGTAT ATTGTGTCAA GAGGAACAAG AGGTTAAGGT GGAAAGCAGG 
GCAATGGTCC TGGCAGCATT TGTTCAAAGA TCAACTGTAT TATCAAAAAA CAGAAGTAAA 
TTCATTCAGG ATCCAGAAAA ATATGATCCA TTATTCATGC ACCCTAATCT GTCTTGTGGA 
ACACACACCA GTAGCTGTGG ACACATTATG CATGCCCATT GTTGGCAAAG GTATTTTGAT 
TCCGTTCAAG CTAAAGAACA GCGAAGGCAA CAGAGATTAC GCTTACATAC AAGCTATGAC 
GTAGAAAATG GAGAATTCCT GTGTCCCCTT TGTGAATGTT TGAGTAACAC TGTTATTCCT 
CTGCTGTTTC CTCCAAGAAA TATTTCTAAC AACAGGTTAA ATTTTTCAGA CCAACCAAAT 
CTGACTCAGT GGATTAGAAC AATATCTCAG CAAATAAAAG CATTGCAGAT TCTTAGGAAA 
GAAGAAAGTA TTCCTAATAT TGTCTCTTCA AAGAATTCAG AAAATATGGA TGAATTGCAG 
CTCCCTGAAG GATTTAGGCC TGATTTTCAT CCTAAGAACC CTTATTCTGA TAGCATAAAA 
GAAATGCTAA CAACGTTTGG AACTGCTACT TACAAGGTGG GACTAAAGGT TCATCCCAAT 
GAAGAAGATC CCCGCGTGCC CATAATGTGT TGGGGTAGTT GTGCGTACAC CATCCAAAGC 
ATAGAAAGAA TCTTAAGTGA TGAAGATAAA CCGTTGTTTG GTCCTTTGCC TTGCAGACTG 
GATGACTGTC TTAGGTCACT AACAAGGTTC GCTGCAGCAC ACTGGACAGT GGCATTACTC 
TCAGTGGTGC AGGAACATTT TTGTAGACTT TTTGCATCAT TGGTGCCTGA TGACAATCAT 
GAAGACCTTC CATGCATATT AGATATCGAC ATGTTTCATT TATTGGTGGG CTTGGTGCTC 
GCTTTCCCCG CGTTGCAGTG TCAGGATTTT TCAGGGATCA GCCTTGAAAC TGGAGACCTT 
CACATTTTCC ATCTGGTTAC TATGGCACAC ATTGTACAGA TCTTACTTAC CTCGTGTACA 
GAAGAGAATG GCATGGATCA AGAAACTCCT GCTGGTGAAG AAGAATTAGC AGTCCTTGCT 
TTGTATAAAA CACTTCGCCA GTACACAGGA AGTGCCTTGA AAGACATACC CTCTGGCTGG 
CATCTGTGGA GGAATGTCAG AGCTGGAATC ATGCCTTTCC TAAAGTGTTC TGCTTTGTTT 
TTTCATTACT TAAATGGAGT TCCTTCTCCA CCTGAAATTC AAGTTTCTGG AACAAGCCAT 
TTTGAACATT TATGTAACTA TCTTTCCCTG CCAAACAACC TCATTTGTCT TTTTCAAGAA 
AATGATGAGA TAATGAAATT ACTGATTGAA AGTTGGTGTC AGAACACTGA AGTTAAAAGA 
TATCTAGAAG GTAAAAGAGA TGCTATCAGC TATCCAAGAG AATCTAACAA ATTAATAGAC 
CTTCCAGAGG ATTACAGCAG CCTCATTAAT CAAGCATCTA ATTTCTCGTG CCCCAAATCA 
GGTGGTGACA AGAGCAGAGC CCCAACTCTG TGCCTTGTGT GTGGAACTCT GCTGTGCTCC 
CAGAGCTACT GCTGTCAGAC CGAACTGGAA GGGGAGGATG TAGGAGCTTG CACAGCTCAC 
ACCTACTCCT GTGGTTCTGG GGTGGGCATC TTCCTGAGAG TACGAGAATG TCAGGTGCTA 
TTTTTAGCCG GCAAAACCAA AGGCTGTTTT TATTCTCCTC CTTACCTTGA TGACTATGGG 
GAGACCGACC AGGGACTCAG ACGGGGAAAT CCTTTACATC TGTGCAAAGA GCGATTTAAG 
AAGATTCAGA AGCTCTGGCA CCAGCACAGT ATCACAGAGG AAATTGGACA TGCGCAGGAA 
GCAAATCAGA CGCTGGTTGG CATTGACTGG CAACATTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006926611.1
CDS	398..5659
Translation	MASELEPEVPAIDRSLLACSAEETAGKWLQATDLTREVYQHLAYYVPKIYCRGPNPLQEEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHKDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHISDVIARTYNIFAIMFRYAVEILTWEKENELPADLEMVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGSIIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAVRFAKNYQQLQRDFMEDDHERAVSVTALSVQFFTAPTLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPIILSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIIMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITQKDVVQQNNTLIEEMLYLIIMLVGERFNPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEEVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNKEDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTILQWAVEHNGYTWSESMLQRVLHLIGMALQEEKQHLENVMEEHIVTFTFTQKISKPGEAPNNCPSILAMLETLQNAPYLEVHKDMIRWILKTFNTIKKMRESSSVSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDVSTSAALDNSPMISDMTFTALGPAQTQVPKQRQFVTCILCQEEQEVKVESRAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPNLSCGTHTSSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLFPPRNISNNRLNFSDQPNLTQWIRTISQQIKALQILRKEESIPNIVSSKNSENMDELQLPEGFRPDFHPKNPYSDSIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVALLSVVQEHFCRLFASLVPDDNHEDLPCILDIDMFHLLVGLVLAFPALQCQDFSGISLETGDLHIFHLVTMAHIVQILLTSCTEENGMDQETPAGEEELAVLALYKTLRQYTGSALKDIPSGWHLWRNVRAGIMPFLKCSALFFHYLNGVPSPPEIQVSGTSHFEHLCNYLSLPNNLICLFQENDEIMKLLIESWCQNTEVKRYLEGKRDAISYPRESNKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGTLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_006926549.3
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006926549.3

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC GGAGGTGCCG GCCATCGACC GGAGTTTGCT GGCATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCT ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT ACAGGAGGAA 
GACATGCTGG CACAGCATGT TTTGTTGGGA CCAATGGAAT GGTACCTTTG TGGTGAAGAT 
CCTGCATTTG GATTTCCAAA ACTTGAACAA GCAAACAAAC CTTCTCATCT TTGTGGTCGT 
GTTTTTAAAG TAGGAGAACC CACATATTCT TGCAGAGACT GTGCAGTTGA TCCAACTTGT 
GTTTTATGCA TGGAGTGCTT TTTGGGAAGT ATTCACAAAG ACCATCGATA TAGGATGACA 
ACATCAGGAG GTGGAGGTTT CTGTGACTGT GGTGATACTG AAGCTTGGAA AGAGGGTCCT 
TACTGTCAAA AACATGAACT TAACACCTCT GAAATTGAGG AAGAAGAGGA TCCTCTTGTG 
CATATATCAG ATGTGATAGC AAGAACTTAC AACATTTTTG CTATTATGTT TCGGTATGCA 
GTGGAGATAC TAACCTGGGA AAAAGAAAAT GAATTGCCAG CAGATTTAGA GATGGTAGAG 
AAGAGTGACA CCTACTATTG CATGCTTTTT AATGATGAGG TTCATACCTA TGAACAAGTT 
ATTTATACTC TTCAGAAAGC TGTTAACTGT ACACAAAAAG AAGCCATTGG CTTTGCAACT 
ACAGTAGATC GAGACGGACG TAGGTCTGTT CGATATGGAG ACTTCCAGTA TTGTGAACAA 
GCCAAATCAG TAATTGTGAG AAATACCAGT AGACAGACAA AGCCACTCAA AGTTCAAGTT 
ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTGTC TTGGCTGGGA 
AGTATTATTG GATATTCAGA TGGCCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GTGAAAACTC TTCTCTTGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTCATGA GCAGTCTTCT TATGGATTTG 
AAATACAAGA AGCTGTTTGC TGTTCGATTT GCAAAGAATT ACCAGCAGTT GCAGAGAGAT 
TTTATGGAGG ATGATCACGA GCGAGCAGTG TCCGTGACTG CTCTGTCTGT CCAGTTCTTC 
ACTGCACCTA CTCTGGCTCG GATGCTCATC ACTGAAGAAA ACCTGATGAC CATTATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA ATTTGAAAGA 
TATACTGCTT TACAAGCTTT CAAATTTAGG AGAGTTCAGA GCCTTATTTT AGACCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTGGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAGGTGGGAC AGCATATTGA AATGGAACCA GAGTGGGAAG CAGCCTTCAC GTTGCAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GATTGGTGTG CTTTAGATGA AAAAGTTTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCAGTA TTGATGCAGT GTCATGGCGG TTTTACTGAT 
GGTGAACAGC CAATCATATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCTCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTGCAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTCAGTGAA 
CTTAGCCCAC CTATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTT 
CATGCAGGAA TGTGGAGAAG AAATGGTTTC TCTCTGGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAA TAATGCTTCA GACAGGTGTC 
TCCATGATGG ACCCAAATCA TTTCCTGATG ATAATGCTCA GCCGCTTTGA ACTTTATCAG 
ATTTTCAGTA CTCCAGACTA CGGAAAAAGA TTTAGTTCTG AGATTACCCA GAAGGATGTA 
GTTCAGCAAA ACAATACTTT AATAGAAGAA ATGTTATACC TCATTATAAT GCTTGTTGGA 
GAGAGATTTA ATCCTGGAGT TGGACAGGTA AATGCTACAG ATGAAATTAA GCGAGAGATT 
ATCCATCAGT TGAGCATCAA ACCTATGGCT CATAGTGAAT TGGTAAAATC TTTACCTGAA 
GATGAGAACA AGGAGACTGG CATGGAGAGT GTCATTGAAG AAGTTGCCCA TTTCAAGAAA 
CCTGGATTAA CAGGACGAGG CATGTATGAA CTGAAACCAG AGTGTGCCAA AGAGTTCAAT 
TTGTATTTCT ATCACTTTTC AAGGGCAGAG CAGTCCAAGG CAGAAGAAGC TCAACGGAAA 
TTGAAAAGAC AAAATAAGGA AGATACAGCA CTTCCACCTC CAGTTTTGCC TCCATTCTGC 
CCTCTGTTCG CAAGTCTGGT TAACATTTTG CAGTCAGATG TCATGTTGTG CATCATGAGA 
ACAATACTGC AGTGGGCTGT GGAACATAAT GGATACACCT GGTCTGAGTC CATGCTGCAA 
AGGGTATTAC ATTTAATTGG AATGGCACTA CAAGAAGAAA AACAGCACTT GGAGAATGTC 
ATGGAAGAGC ATATAGTAAC ATTTACCTTC ACTCAGAAGA TCTCAAAACC TGGTGAAGCA 
CCAAACAACT GTCCTAGTAT ACTAGCTATG CTGGAAACAC TGCAAAATGC TCCCTACCTA 
GAAGTCCACA AAGATATGAT TAGATGGATA TTAAAGACTT TTAATACTAT TAAGAAAATG 
AGGGAGAGTT CATCTGTCAG TCCTGTAGCA GAGACAGAAG GAACCATAAT GGAAGAGAGT 
TCAAGAGACA AAGACAAAGC TGAGAGGAAG AGAAAAGCTG AGATTGCCAG ACTGCGCAGA 
GAAAAGATCA TGGCCCAGAT GTCTGAGATG CAGCGGCACT TCATTGATGA GAACAAAGAA 
CTCTTCCAGC AGACATTAGA ACTGGATGTC TCCACCTCTG CTGCTCTTGA TAATAGCCCT 
ATGATTTCAG ATATGACATT TACAGCATTG GGCCCAGCAC AAACTCAGGT CCCTAAACAA 
AGACAATTTG TTACCTGTAT ATTGTGTCAA GAGGAACAAG AGGTTAAGGT GGAAAGCAGG 
GCAATGGTCC TGGCAGCATT TGTTCAAAGA TCAACTGTAT TATCAAAAAA CAGAAGTAAA 
TTCATTCAGG ATCCAGAAAA ATATGATCCA TTATTCATGC ACCCTAATCT GTCTTGTGGA 
ACACACACCA GTAGCTGTGG ACACATTATG CATGCCCATT GTTGGCAAAG GTATTTTGAT 
TCCGTTCAAG CTAAAGAACA GCGAAGGCAA CAGAGATTAC GCTTACATAC AAGCTATGAC 
GTAGAAAATG GAGAATTCCT GTGTCCCCTT TGTGAATGTT TGAGTAACAC TGTTATTCCT 
CTGCTGTTTC CTCCAAGAAA TATTTCTAAC AACAGGTTAA ATTTTTCAGA CCAACCAAAT 
CTGACTCAGT GGATTAGAAC AATATCTCAG CAAATAAAAG CATTGCAGAT TCTTAGGAAA 
GAAGAAAGTA TTCCTAATAT TGTCTCTTCA AAGAATTCAG AAAATATGGA TGAATTGCAG 
CTCCCTGAAG GATTTAGGCC TGATTTTCAT CCTAAGAACC CTTATTCTGA TAGCATAAAA 
GAAATGCTAA CAACGTTTGG AACTGCTACT TACAAGGTGG GACTAAAGGT TCATCCCAAT 
GAAGAAGATC CCCGCGTGCC CATAATGTGT TGGGGTAGTT GTGCGTACAC CATCCAAAGC 
ATAGAAAGAA TCTTAAGTGA TGAAGATAAA CCGTTGTTTG GTCCTTTGCC TTGCAGACTG 
GATGACTGTC TTAGGTCACT AACAAGGTTC GCTGCAGCAC ACTGGACAGT GGCATTACTC 
TCAGTGGTGC AGGAACATTT TTGTAGACTT TTTGCATCAT TGGTGCCTGA TGACAATCAT 
GAAGACCTTC CATGCATATT AGATATCGAC ATGTTTCATT TATTGGTGGG CTTGGTGCTC 
GCTTTCCCCG CGTTGCAGTG TCAGGATTTT TCAGGGATCA GCCTTGAAAC TGGAGACCTT 
CACATTTTCC ATCTGGTTAC TATGGCACAC ATTGTACAGA TCTTACTTAC CTCGTGTACA 
GAAGAGAATG GCATGGATCA AGAAACTCCT GCTGGTGAAG AAGAATTAGC AGTCCTTGCT 
TTGTATAAAA CACTTCGCCA GTACACAGGA AGTGCCTTGA AAGACATACC CTCTGGCTGG 
CATCTGTGGA GGAATGTCAG AGCTGGAATC ATGCCTTTCC TAAAGTGTTC TGCTTTGTTT 
TTTCATTACT TAAATGGAGT TCCTTCTCCA CCTGAAATTC AAGTTTCTGG AACAAGCCAT 
TTTGAACATT TATGTAACTA TCTTTCCCTG CCAAACAACC TCATTTGTCT TTTTCAAGAA 
AATGATGAGA TAATGAAATT ACTGATTGAA AGTTGGTGTC AGAACACTGA AGTTAAAAGA 
TATCTAGAAG GTAAAAGAGA TGCTATCAGC TATCCAAGAG AATCTAACAA ATTAATAGAC 
CTTCCAGAGG ATTACAGCAG CCTCATTAAT CAAGCATCTA ATTTCTCGTG CCCCAAATCA 
GGTGGTGACA AGAGCAGAGC CCCAACTCTG TGCCTTGTGT GTGGAACTCT GCTGTGCTCC 
CAGAGCTACT GCTGTCAGAC CGAACTGGAA GGGGAGGATG TAGGAGCTTG CACAGCTCAC 
ACCTACTCCT GTGGTTCTGG GGTGGGCATC TTCCTGAGAG TACGAGAATG TCAGGTGCTA 
TTTTTAGCCG GCAAAACCAA AGGCTGTTTT TATTCTCCTC CTTACCTTGA TGACTATGGG 
GAGACCGACC AGGGACTCAG ACGGGGAAAT CCTTTACATC TGTGCAAAGA GCGATTTAAG 
AAGATTCAGA AGCTCTGGCA CCAGCACAGT ATCACAGAGG AAATTGGACA TGCGCAGGAA 
GCAAATCAGA CGCTGGTTGG CATTGACTGG CAACATTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_006926550.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006926612.1 Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPt31992
Clone ID Related Accession (Same CDS sequence)	XM_006926550.2 , XM_006926550.3
Accession Version	XM_006926550.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	E3 ubiquitin-protein ligase UBR2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 10, 2016 this sequence version replaced XM_006926550.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC GGAGGTGCCG GCCATCGACC GGAGTTTGCT GGCATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCT ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT ACAGGAGGAA 
GACATGCTGG CACAGCATGT TTTGTTGGGA CCAATGGAAT GGTACCTTTG TGGTGAAGAT 
CCTGCATTTG GATTTCCAAA ACTTGAACAA GCAAACAAAC CTTCTCATCT TTGTGGTCGT 
GTTTTTAAAG TAGGAGAACC CACATATTCT TGCAGAGACT GTGCAGTTGA TCCAACTTGT 
GTTTTATGCA TGGAGTGCTT TTTGGGAAGT ATTCACAAAG ACCATCGATA TAGGATGACA 
ACATCAGGAG GTGGAGGTTT CTGTGACTGT GGTGATACTG AAGCTTGGAA AGAGGGTCCT 
TACTGTCAAA AACATGAACT TAACACCTCT GAAATTGAGG AAGAAGAGGA TCCTCTTGTG 
CATATATCAG ATGTGATAGC AAGAACTTAC AACATTTTTG CTATTATGTT TCGGTATGCA 
GTGGAGATAC TAACCTGGGA AAAAGAAAAT GAATTGCCAG CAGATTTAGA GATGGTAGAG 
AAGAGTGACA CCTACTATTG CATGCTTTTT AATGATGAGG TTCATACCTA TGAACAAGTT 
ATTTATACTC TTCAGAAAGC TGTTAACTGT ACACAAAAAG AAGCCATTGG CTTTGCAACT 
ACAGTAGATC GAGACGGACG TAGGTCTGTT CGATATGGAG ACTTCCAGTA TTGTGAACAA 
GCCAAATCAG TAATTGTGAG AAATACCAGT AGACAGACAA AGCCACTCAA AGTTCAAGTT 
ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTGTC TTGGCTGGGA 
AGTATTATTG GATATTCAGA TGGCCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GTGAAAACTC TTCTCTTGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTCATGA GCAGTCTTCT TATGGATTTG 
AAATACAAGA AGCTGTTTGC TGTTCGATTT GCAAAGAACT ATGAGCGTTT GCAGAGTGAT 
TATGTGACAG ATGACCACGA CAGAGAGTTT TCAGTCGCAG ACCTCTCGGT TCAGATATTC 
ACAGTTCCTT CACTTGCTCG GATGCTCATC ACTGAAGAAA ACCTGATGAC CATTATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA ATTTGAAAGA 
TATACTGCTT TACAAGCTTT CAAATTTAGG AGAGTTCAGA GCCTTATTTT AGACCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTGGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAGGTGGGAC AGCATATTGA AATGGAACCA GAGTGGGAAG CAGCCTTCAC GTTGCAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GATTGGTGTG CTTTAGATGA AAAAGTTTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCAGTA TTGATGCAGT GTCATGGCGG TTTTACTGAT 
GGTGAACAGC CAATCATATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCTCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTGCAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTCAGTGAA 
CTTAGCCCAC CTATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTT 
CATGCAGGAA TGTGGAGAAG AAATGGTTTC TCTCTGGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAA TAATGCTTCA GACAGGTGTC 
TCCATGATGG ACCCAAATCA TTTCCTGATG ATAATGCTCA GCCGCTTTGA ACTTTATCAG 
ATTTTCAGTA CTCCAGACTA CGGAAAAAGA TTTAGTTCTG AGATTACCCA GAAGGATGTA 
GTTCAGCAAA ACAATACTTT AATAGAAGAA ATGTTATACC TCATTATAAT GCTTGTTGGA 
GAGAGATTTA ATCCTGGAGT TGGACAGGTA AATGCTACAG ATGAAATTAA GCGAGAGATT 
ATCCATCAGT TGAGCATCAA ACCTATGGCT CATAGTGAAT TGGTAAAATC TTTACCTGAA 
GATGAGAACA AGGAGACTGG CATGGAGAGT GTCATTGAAG AAGTTGCCCA TTTCAAGAAA 
CCTGGATTAA CAGGACGAGG CATGTATGAA CTGAAACCAG AGTGTGCCAA AGAGTTCAAT 
TTGTATTTCT ATCACTTTTC AAGGGCAGAG CAGTCCAAGG CAGAAGAAGC TCAACGGAAA 
TTGAAAAGAC AAAATAAGGA AGATACAGCA CTTCCACCTC CAGTTTTGCC TCCATTCTGC 
CCTCTGTTCG CAAGTCTGGT TAACATTTTG CAGTCAGATG TCATGTTGTG CATCATGAGA 
ACAATACTGC AGTGGGCTGT GGAACATAAT GGATACACCT GGTCTGAGTC CATGCTGCAA 
AGGGTATTAC ATTTAATTGG AATGGCACTA CAAGAAGAAA AACAGCACTT GGAGAATGTC 
ATGGAAGAGC ATATAGTAAC ATTTACCTTC ACTCAGAAGA TCTCAAAACC TGGTGAAGCA 
CCAAACAACT GTCCTAGTAT ACTAGCTATG CTGGAAACAC TGCAAAATGC TCCCTACCTA 
GAAGTCCACA AAGATATGAT TAGATGGATA TTAAAGACTT TTAATACTAT TAAGAAAATG 
AGGGAGAGTT CATCTGTCAG TCCTGTAGCA GAGACAGAAG GAACCATAAT GGAAGAGAGT 
TCAAGAGACA AAGACAAAGC TGAGAGGAAG AGAAAAGCTG AGATTGCCAG ACTGCGCAGA 
GAAAAGATCA TGGCCCAGAT GTCTGAGATG CAGCGGCACT TCATTGATGA GAACAAAGAA 
CTCTTCCAGC AGACATTAGA ACTGGATGTC TCCACCTCTG CTGCTCTTGA TAATAGCCCT 
ATGATTTCAG ATATGACATT TACAGCATTG GGCCCAGCAC AAACTCAGGT CCCTAAACAA 
AGACAATTTG TTACCTGTAT ATTGTGTCAA GAGGAACAAG AGGTTAAGGT GGAAAGCAGG 
GCAATGGTCC TGGCAGCATT TGTTCAAAGA TCAACTGTAT TATCAAAAAA CAGAAGTAAA 
TTCATTCAGG ATCCAGAAAA ATATGATCCA TTATTCATGC ACCCTAATCT GTCTTGTGGA 
ACACACACCA GTAGCTGTGG ACACATTATG CATGCCCATT GTTGGCAAAG GTATTTTGAT 
TCCGTTCAAG CTAAAGAACA GCGAAGGCAA CAGAGATTAC GCTTACATAC AAGCTATGAC 
GTAGAAAATG GAGAATTCCT GTGTCCCCTT TGTGAATGTT TGAGTAACAC TGTTATTCCT 
CTGCTGTTTC CTCCAAGAAA TATTTCTAAC AACAGGTTAA ATTTTTCAGA CCAACCAAAT 
CTGACTCAGT GGATTAGAAC AATATCTCAG CAAATAAAAG CATTGCAGAT TCTTAGGAAA 
GAAGAAAGTA TTCCTAATAT TGTCTCTTCA AAGAATTCAG AAAATATGGA TGAATTGCAG 
CTCCCTGAAG GATTTAGGCC TGATTTTCAT CCTAAGAACC CTTATTCTGA TAGCATAAAA 
GAAATGCTAA CAACGTTTGG AACTGCTACT TACAAGGTGG GACTAAAGGT TCATCCCAAT 
GAAGAAGATC CCCGCGTGCC CATAATGTGT TGGGGTAGTT GTGCGTACAC CATCCAAAGC 
ATAGAAAGAA TCTTAAGTGA TGAAGATAAA CCGTTGTTTG GTCCTTTGCC TTGCAGACTG 
GATGACTGTC TTAGGTCACT AACAAGGTTC GCTGCAGCAC ACTGGACAGT GGCATTACTC 
TCAGTGGTGC AGGAACATTT TTGTAGACTT TTTGCATCAT TGGTGCCTGA TGACAATCAT 
GAAGACCTTC CATGCATATT AGATATCGAC ATGTTTCATT TATTGGTGGG CTTGGTGCTC 
GCTTTCCCCG CGTTGCAGTG TCAGGATTTT TCAGGGATCA GCCTTGAAAC TGGAGACCTT 
CACATTTTCC ATCTGGTTAC TATGGCACAC ATTGTACAGA TCTTACTTAC CTCGTGTACA 
GAAGAGAATG GCATGGATCA AGAAACTCCT GCTGGTGAAG AAGAATTAGC AGTCCTTGCT 
TTGTATAAAA CACTTCGCCA GTACACAGGA AGTGCCTTGA AAGACATACC CTCTGGCTGG 
CATCTGTGGA GGAATGTCAG AGCTGGAATC ATGCCTTTCC TAAAGTGTTC TGCTTTGTTT 
TTTCATTACT TAAATGGAGT TCCTTCTCCA CCTGAAATTC AAGTTTCTGG AACAAGCCAT 
TTTGAACATT TATGTAACTA TCTTTCCCTG CCAAACAACC TCATTTGTCT TTTTCAAGAA 
AATGATGAGA TAATGAAATT ACTGATTGAA AGTTGGTGTC AGAACACTGA AGTTAAAAGA 
TATCTAGAAG GTAAAAGAGA TGCTATCAGC TATCCAAGAG AATCTAACAA ATTAATAGAC 
CTTCCAGAGG ATTACAGCAG CCTCATTAAT CAAGCATCTA ATTTCTCGTG CCCCAAATCA 
GGTGGTGACA AGAGCAGAGC CCCAACTCTG TGCCTTGTGT GTGGAACTCT GCTGTGCTCC 
CAGAGCTACT GCTGTCAGAC CGAACTGGAA GGGGAGGATG TAGGAGCTTG CACAGCTCAC 
ACCTACTCCT GTGGTTCTGG GGTGGGCATC TTCCTGAGAG TACGAGAATG TCAGGTGCTA 
TTTTTAGCCG GCAAAACCAA AGGCTGTTTT TATTCTCCTC CTTACCTTGA TGACTATGGG 
GAGACCGACC AGGGACTCAG ACGGGGAAAT CCTTTACATC TGTGCAAAGA GCGATTTAAG 
AAGATTCAGA AGCTCTGGCA CCAGCACAGT ATCACAGAGG AAATTGGACA TGCGCAGGAA 
GCAAATCAGA CGCTGGTTGG CATTGACTGG CAACATTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006926612.1
CDS	365..5626
Translation	MASELEPEVPAIDRSLLACSAEETAGKWLQATDLTREVYQHLAYYVPKIYCRGPNPLQEEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHKDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHISDVIARTYNIFAIMFRYAVEILTWEKENELPADLEMVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGSIIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAVRFAKNYERLQSDYVTDDHDREFSVADLSVQIFTVPSLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPIILSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIIMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITQKDVVQQNNTLIEEMLYLIIMLVGERFNPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEEVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNKEDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTILQWAVEHNGYTWSESMLQRVLHLIGMALQEEKQHLENVMEEHIVTFTFTQKISKPGEAPNNCPSILAMLETLQNAPYLEVHKDMIRWILKTFNTIKKMRESSSVSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDVSTSAALDNSPMISDMTFTALGPAQTQVPKQRQFVTCILCQEEQEVKVESRAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPNLSCGTHTSSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLFPPRNISNNRLNFSDQPNLTQWIRTISQQIKALQILRKEESIPNIVSSKNSENMDELQLPEGFRPDFHPKNPYSDSIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVALLSVVQEHFCRLFASLVPDDNHEDLPCILDIDMFHLLVGLVLAFPALQCQDFSGISLETGDLHIFHLVTMAHIVQILLTSCTEENGMDQETPAGEEELAVLALYKTLRQYTGSALKDIPSGWHLWRNVRAGIMPFLKCSALFFHYLNGVPSPPEIQVSGTSHFEHLCNYLSLPNNLICLFQENDEIMKLLIESWCQNTEVKRYLEGKRDAISYPRESNKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGTLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_006926550.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006926550.2

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC GGAGGTGCCG GCCATCGACC GGAGTTTGCT GGCATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCT ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT ACAGGAGGAA 
GACATGCTGG CACAGCATGT TTTGTTGGGA CCAATGGAAT GGTACCTTTG TGGTGAAGAT 
CCTGCATTTG GATTTCCAAA ACTTGAACAA GCAAACAAAC CTTCTCATCT TTGTGGTCGT 
GTTTTTAAAG TAGGAGAACC CACATATTCT TGCAGAGACT GTGCAGTTGA TCCAACTTGT 
GTTTTATGCA TGGAGTGCTT TTTGGGAAGT ATTCACAAAG ACCATCGATA TAGGATGACA 
ACATCAGGAG GTGGAGGTTT CTGTGACTGT GGTGATACTG AAGCTTGGAA AGAGGGTCCT 
TACTGTCAAA AACATGAACT TAACACCTCT GAAATTGAGG AAGAAGAGGA TCCTCTTGTG 
CATATATCAG ATGTGATAGC AAGAACTTAC AACATTTTTG CTATTATGTT TCGGTATGCA 
GTGGAGATAC TAACCTGGGA AAAAGAAAAT GAATTGCCAG CAGATTTAGA GATGGTAGAG 
AAGAGTGACA CCTACTATTG CATGCTTTTT AATGATGAGG TTCATACCTA TGAACAAGTT 
ATTTATACTC TTCAGAAAGC TGTTAACTGT ACACAAAAAG AAGCCATTGG CTTTGCAACT 
ACAGTAGATC GAGACGGACG TAGGTCTGTT CGATATGGAG ACTTCCAGTA TTGTGAACAA 
GCCAAATCAG TAATTGTGAG AAATACCAGT AGACAGACAA AGCCACTCAA AGTTCAAGTT 
ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTGTC TTGGCTGGGA 
AGTATTATTG GATATTCAGA TGGCCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GTGAAAACTC TTCTCTTGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTCATGA GCAGTCTTCT TATGGATTTG 
AAATACAAGA AGCTGTTTGC TGTTCGATTT GCAAAGAACT ATGAGCGTTT GCAGAGTGAT 
TATGTGACAG ATGACCACGA CAGAGAGTTT TCAGTCGCAG ACCTCTCGGT TCAGATATTC 
ACAGTTCCTT CACTTGCTCG GATGCTCATC ACTGAAGAAA ACCTGATGAC CATTATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA ATTTGAAAGA 
TATACTGCTT TACAAGCTTT CAAATTTAGG AGAGTTCAGA GCCTTATTTT AGACCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTGGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAGGTGGGAC AGCATATTGA AATGGAACCA GAGTGGGAAG CAGCCTTCAC GTTGCAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GATTGGTGTG CTTTAGATGA AAAAGTTTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCAGTA TTGATGCAGT GTCATGGCGG TTTTACTGAT 
GGTGAACAGC CAATCATATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCTCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTGCAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTCAGTGAA 
CTTAGCCCAC CTATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTT 
CATGCAGGAA TGTGGAGAAG AAATGGTTTC TCTCTGGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAA TAATGCTTCA GACAGGTGTC 
TCCATGATGG ACCCAAATCA TTTCCTGATG ATAATGCTCA GCCGCTTTGA ACTTTATCAG 
ATTTTCAGTA CTCCAGACTA CGGAAAAAGA TTTAGTTCTG AGATTACCCA GAAGGATGTA 
GTTCAGCAAA ACAATACTTT AATAGAAGAA ATGTTATACC TCATTATAAT GCTTGTTGGA 
GAGAGATTTA ATCCTGGAGT TGGACAGGTA AATGCTACAG ATGAAATTAA GCGAGAGATT 
ATCCATCAGT TGAGCATCAA ACCTATGGCT CATAGTGAAT TGGTAAAATC TTTACCTGAA 
GATGAGAACA AGGAGACTGG CATGGAGAGT GTCATTGAAG AAGTTGCCCA TTTCAAGAAA 
CCTGGATTAA CAGGACGAGG CATGTATGAA CTGAAACCAG AGTGTGCCAA AGAGTTCAAT 
TTGTATTTCT ATCACTTTTC AAGGGCAGAG CAGTCCAAGG CAGAAGAAGC TCAACGGAAA 
TTGAAAAGAC AAAATAAGGA AGATACAGCA CTTCCACCTC CAGTTTTGCC TCCATTCTGC 
CCTCTGTTCG CAAGTCTGGT TAACATTTTG CAGTCAGATG TCATGTTGTG CATCATGAGA 
ACAATACTGC AGTGGGCTGT GGAACATAAT GGATACACCT GGTCTGAGTC CATGCTGCAA 
AGGGTATTAC ATTTAATTGG AATGGCACTA CAAGAAGAAA AACAGCACTT GGAGAATGTC 
ATGGAAGAGC ATATAGTAAC ATTTACCTTC ACTCAGAAGA TCTCAAAACC TGGTGAAGCA 
CCAAACAACT GTCCTAGTAT ACTAGCTATG CTGGAAACAC TGCAAAATGC TCCCTACCTA 
GAAGTCCACA AAGATATGAT TAGATGGATA TTAAAGACTT TTAATACTAT TAAGAAAATG 
AGGGAGAGTT CATCTGTCAG TCCTGTAGCA GAGACAGAAG GAACCATAAT GGAAGAGAGT 
TCAAGAGACA AAGACAAAGC TGAGAGGAAG AGAAAAGCTG AGATTGCCAG ACTGCGCAGA 
GAAAAGATCA TGGCCCAGAT GTCTGAGATG CAGCGGCACT TCATTGATGA GAACAAAGAA 
CTCTTCCAGC AGACATTAGA ACTGGATGTC TCCACCTCTG CTGCTCTTGA TAATAGCCCT 
ATGATTTCAG ATATGACATT TACAGCATTG GGCCCAGCAC AAACTCAGGT CCCTAAACAA 
AGACAATTTG TTACCTGTAT ATTGTGTCAA GAGGAACAAG AGGTTAAGGT GGAAAGCAGG 
GCAATGGTCC TGGCAGCATT TGTTCAAAGA TCAACTGTAT TATCAAAAAA CAGAAGTAAA 
TTCATTCAGG ATCCAGAAAA ATATGATCCA TTATTCATGC ACCCTAATCT GTCTTGTGGA 
ACACACACCA GTAGCTGTGG ACACATTATG CATGCCCATT GTTGGCAAAG GTATTTTGAT 
TCCGTTCAAG CTAAAGAACA GCGAAGGCAA CAGAGATTAC GCTTACATAC AAGCTATGAC 
GTAGAAAATG GAGAATTCCT GTGTCCCCTT TGTGAATGTT TGAGTAACAC TGTTATTCCT 
CTGCTGTTTC CTCCAAGAAA TATTTCTAAC AACAGGTTAA ATTTTTCAGA CCAACCAAAT 
CTGACTCAGT GGATTAGAAC AATATCTCAG CAAATAAAAG CATTGCAGAT TCTTAGGAAA 
GAAGAAAGTA TTCCTAATAT TGTCTCTTCA AAGAATTCAG AAAATATGGA TGAATTGCAG 
CTCCCTGAAG GATTTAGGCC TGATTTTCAT CCTAAGAACC CTTATTCTGA TAGCATAAAA 
GAAATGCTAA CAACGTTTGG AACTGCTACT TACAAGGTGG GACTAAAGGT TCATCCCAAT 
GAAGAAGATC CCCGCGTGCC CATAATGTGT TGGGGTAGTT GTGCGTACAC CATCCAAAGC 
ATAGAAAGAA TCTTAAGTGA TGAAGATAAA CCGTTGTTTG GTCCTTTGCC TTGCAGACTG 
GATGACTGTC TTAGGTCACT AACAAGGTTC GCTGCAGCAC ACTGGACAGT GGCATTACTC 
TCAGTGGTGC AGGAACATTT TTGTAGACTT TTTGCATCAT TGGTGCCTGA TGACAATCAT 
GAAGACCTTC CATGCATATT AGATATCGAC ATGTTTCATT TATTGGTGGG CTTGGTGCTC 
GCTTTCCCCG CGTTGCAGTG TCAGGATTTT TCAGGGATCA GCCTTGAAAC TGGAGACCTT 
CACATTTTCC ATCTGGTTAC TATGGCACAC ATTGTACAGA TCTTACTTAC CTCGTGTACA 
GAAGAGAATG GCATGGATCA AGAAACTCCT GCTGGTGAAG AAGAATTAGC AGTCCTTGCT 
TTGTATAAAA CACTTCGCCA GTACACAGGA AGTGCCTTGA AAGACATACC CTCTGGCTGG 
CATCTGTGGA GGAATGTCAG AGCTGGAATC ATGCCTTTCC TAAAGTGTTC TGCTTTGTTT 
TTTCATTACT TAAATGGAGT TCCTTCTCCA CCTGAAATTC AAGTTTCTGG AACAAGCCAT 
TTTGAACATT TATGTAACTA TCTTTCCCTG CCAAACAACC TCATTTGTCT TTTTCAAGAA 
AATGATGAGA TAATGAAATT ACTGATTGAA AGTTGGTGTC AGAACACTGA AGTTAAAAGA 
TATCTAGAAG GTAAAAGAGA TGCTATCAGC TATCCAAGAG AATCTAACAA ATTAATAGAC 
CTTCCAGAGG ATTACAGCAG CCTCATTAAT CAAGCATCTA ATTTCTCGTG CCCCAAATCA 
GGTGGTGACA AGAGCAGAGC CCCAACTCTG TGCCTTGTGT GTGGAACTCT GCTGTGCTCC 
CAGAGCTACT GCTGTCAGAC CGAACTGGAA GGGGAGGATG TAGGAGCTTG CACAGCTCAC 
ACCTACTCCT GTGGTTCTGG GGTGGGCATC TTCCTGAGAG TACGAGAATG TCAGGTGCTA 
TTTTTAGCCG GCAAAACCAA AGGCTGTTTT TATTCTCCTC CTTACCTTGA TGACTATGGG 
GAGACCGACC AGGGACTCAG ACGGGGAAAT CCTTTACATC TGTGCAAAGA GCGATTTAAG 
AAGATTCAGA AGCTCTGGCA CCAGCACAGT ATCACAGAGG AAATTGGACA TGCGCAGGAA 
GCAAATCAGA CGCTGGTTGG CATTGACTGG CAACATTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_006926550.3 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006926612.1 Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.

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CloneID	OPt31992
Clone ID Related Accession (Same CDS sequence)	XM_006926550.2 , XM_006926550.3
Accession Version	XM_006926550.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	E3 ubiquitin-protein ligase UBR2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_006926550.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC GGAGGTGCCG GCCATCGACC GGAGTTTGCT GGCATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCT ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT ACAGGAGGAA 
GACATGCTGG CACAGCATGT TTTGTTGGGA CCAATGGAAT GGTACCTTTG TGGTGAAGAT 
CCTGCATTTG GATTTCCAAA ACTTGAACAA GCAAACAAAC CTTCTCATCT TTGTGGTCGT 
GTTTTTAAAG TAGGAGAACC CACATATTCT TGCAGAGACT GTGCAGTTGA TCCAACTTGT 
GTTTTATGCA TGGAGTGCTT TTTGGGAAGT ATTCACAAAG ACCATCGATA TAGGATGACA 
ACATCAGGAG GTGGAGGTTT CTGTGACTGT GGTGATACTG AAGCTTGGAA AGAGGGTCCT 
TACTGTCAAA AACATGAACT TAACACCTCT GAAATTGAGG AAGAAGAGGA TCCTCTTGTG 
CATATATCAG ATGTGATAGC AAGAACTTAC AACATTTTTG CTATTATGTT TCGGTATGCA 
GTGGAGATAC TAACCTGGGA AAAAGAAAAT GAATTGCCAG CAGATTTAGA GATGGTAGAG 
AAGAGTGACA CCTACTATTG CATGCTTTTT AATGATGAGG TTCATACCTA TGAACAAGTT 
ATTTATACTC TTCAGAAAGC TGTTAACTGT ACACAAAAAG AAGCCATTGG CTTTGCAACT 
ACAGTAGATC GAGACGGACG TAGGTCTGTT CGATATGGAG ACTTCCAGTA TTGTGAACAA 
GCCAAATCAG TAATTGTGAG AAATACCAGT AGACAGACAA AGCCACTCAA AGTTCAAGTT 
ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTGTC TTGGCTGGGA 
AGTATTATTG GATATTCAGA TGGCCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GTGAAAACTC TTCTCTTGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTCATGA GCAGTCTTCT TATGGATTTG 
AAATACAAGA AGCTGTTTGC TGTTCGATTT GCAAAGAACT ATGAGCGTTT GCAGAGTGAT 
TATGTGACAG ATGACCACGA CAGAGAGTTT TCAGTCGCAG ACCTCTCGGT TCAGATATTC 
ACAGTTCCTT CACTTGCTCG GATGCTCATC ACTGAAGAAA ACCTGATGAC CATTATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA ATTTGAAAGA 
TATACTGCTT TACAAGCTTT CAAATTTAGG AGAGTTCAGA GCCTTATTTT AGACCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTGGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAGGTGGGAC AGCATATTGA AATGGAACCA GAGTGGGAAG CAGCCTTCAC GTTGCAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GATTGGTGTG CTTTAGATGA AAAAGTTTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCAGTA TTGATGCAGT GTCATGGCGG TTTTACTGAT 
GGTGAACAGC CAATCATATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCTCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTGCAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTCAGTGAA 
CTTAGCCCAC CTATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTT 
CATGCAGGAA TGTGGAGAAG AAATGGTTTC TCTCTGGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAA TAATGCTTCA GACAGGTGTC 
TCCATGATGG ACCCAAATCA TTTCCTGATG ATAATGCTCA GCCGCTTTGA ACTTTATCAG 
ATTTTCAGTA CTCCAGACTA CGGAAAAAGA TTTAGTTCTG AGATTACCCA GAAGGATGTA 
GTTCAGCAAA ACAATACTTT AATAGAAGAA ATGTTATACC TCATTATAAT GCTTGTTGGA 
GAGAGATTTA ATCCTGGAGT TGGACAGGTA AATGCTACAG ATGAAATTAA GCGAGAGATT 
ATCCATCAGT TGAGCATCAA ACCTATGGCT CATAGTGAAT TGGTAAAATC TTTACCTGAA 
GATGAGAACA AGGAGACTGG CATGGAGAGT GTCATTGAAG AAGTTGCCCA TTTCAAGAAA 
CCTGGATTAA CAGGACGAGG CATGTATGAA CTGAAACCAG AGTGTGCCAA AGAGTTCAAT 
TTGTATTTCT ATCACTTTTC AAGGGCAGAG CAGTCCAAGG CAGAAGAAGC TCAACGGAAA 
TTGAAAAGAC AAAATAAGGA AGATACAGCA CTTCCACCTC CAGTTTTGCC TCCATTCTGC 
CCTCTGTTCG CAAGTCTGGT TAACATTTTG CAGTCAGATG TCATGTTGTG CATCATGAGA 
ACAATACTGC AGTGGGCTGT GGAACATAAT GGATACACCT GGTCTGAGTC CATGCTGCAA 
AGGGTATTAC ATTTAATTGG AATGGCACTA CAAGAAGAAA AACAGCACTT GGAGAATGTC 
ATGGAAGAGC ATATAGTAAC ATTTACCTTC ACTCAGAAGA TCTCAAAACC TGGTGAAGCA 
CCAAACAACT GTCCTAGTAT ACTAGCTATG CTGGAAACAC TGCAAAATGC TCCCTACCTA 
GAAGTCCACA AAGATATGAT TAGATGGATA TTAAAGACTT TTAATACTAT TAAGAAAATG 
AGGGAGAGTT CATCTGTCAG TCCTGTAGCA GAGACAGAAG GAACCATAAT GGAAGAGAGT 
TCAAGAGACA AAGACAAAGC TGAGAGGAAG AGAAAAGCTG AGATTGCCAG ACTGCGCAGA 
GAAAAGATCA TGGCCCAGAT GTCTGAGATG CAGCGGCACT TCATTGATGA GAACAAAGAA 
CTCTTCCAGC AGACATTAGA ACTGGATGTC TCCACCTCTG CTGCTCTTGA TAATAGCCCT 
ATGATTTCAG ATATGACATT TACAGCATTG GGCCCAGCAC AAACTCAGGT CCCTAAACAA 
AGACAATTTG TTACCTGTAT ATTGTGTCAA GAGGAACAAG AGGTTAAGGT GGAAAGCAGG 
GCAATGGTCC TGGCAGCATT TGTTCAAAGA TCAACTGTAT TATCAAAAAA CAGAAGTAAA 
TTCATTCAGG ATCCAGAAAA ATATGATCCA TTATTCATGC ACCCTAATCT GTCTTGTGGA 
ACACACACCA GTAGCTGTGG ACACATTATG CATGCCCATT GTTGGCAAAG GTATTTTGAT 
TCCGTTCAAG CTAAAGAACA GCGAAGGCAA CAGAGATTAC GCTTACATAC AAGCTATGAC 
GTAGAAAATG GAGAATTCCT GTGTCCCCTT TGTGAATGTT TGAGTAACAC TGTTATTCCT 
CTGCTGTTTC CTCCAAGAAA TATTTCTAAC AACAGGTTAA ATTTTTCAGA CCAACCAAAT 
CTGACTCAGT GGATTAGAAC AATATCTCAG CAAATAAAAG CATTGCAGAT TCTTAGGAAA 
GAAGAAAGTA TTCCTAATAT TGTCTCTTCA AAGAATTCAG AAAATATGGA TGAATTGCAG 
CTCCCTGAAG GATTTAGGCC TGATTTTCAT CCTAAGAACC CTTATTCTGA TAGCATAAAA 
GAAATGCTAA CAACGTTTGG AACTGCTACT TACAAGGTGG GACTAAAGGT TCATCCCAAT 
GAAGAAGATC CCCGCGTGCC CATAATGTGT TGGGGTAGTT GTGCGTACAC CATCCAAAGC 
ATAGAAAGAA TCTTAAGTGA TGAAGATAAA CCGTTGTTTG GTCCTTTGCC TTGCAGACTG 
GATGACTGTC TTAGGTCACT AACAAGGTTC GCTGCAGCAC ACTGGACAGT GGCATTACTC 
TCAGTGGTGC AGGAACATTT TTGTAGACTT TTTGCATCAT TGGTGCCTGA TGACAATCAT 
GAAGACCTTC CATGCATATT AGATATCGAC ATGTTTCATT TATTGGTGGG CTTGGTGCTC 
GCTTTCCCCG CGTTGCAGTG TCAGGATTTT TCAGGGATCA GCCTTGAAAC TGGAGACCTT 
CACATTTTCC ATCTGGTTAC TATGGCACAC ATTGTACAGA TCTTACTTAC CTCGTGTACA 
GAAGAGAATG GCATGGATCA AGAAACTCCT GCTGGTGAAG AAGAATTAGC AGTCCTTGCT 
TTGTATAAAA CACTTCGCCA GTACACAGGA AGTGCCTTGA AAGACATACC CTCTGGCTGG 
CATCTGTGGA GGAATGTCAG AGCTGGAATC ATGCCTTTCC TAAAGTGTTC TGCTTTGTTT 
TTTCATTACT TAAATGGAGT TCCTTCTCCA CCTGAAATTC AAGTTTCTGG AACAAGCCAT 
TTTGAACATT TATGTAACTA TCTTTCCCTG CCAAACAACC TCATTTGTCT TTTTCAAGAA 
AATGATGAGA TAATGAAATT ACTGATTGAA AGTTGGTGTC AGAACACTGA AGTTAAAAGA 
TATCTAGAAG GTAAAAGAGA TGCTATCAGC TATCCAAGAG AATCTAACAA ATTAATAGAC 
CTTCCAGAGG ATTACAGCAG CCTCATTAAT CAAGCATCTA ATTTCTCGTG CCCCAAATCA 
GGTGGTGACA AGAGCAGAGC CCCAACTCTG TGCCTTGTGT GTGGAACTCT GCTGTGCTCC 
CAGAGCTACT GCTGTCAGAC CGAACTGGAA GGGGAGGATG TAGGAGCTTG CACAGCTCAC 
ACCTACTCCT GTGGTTCTGG GGTGGGCATC TTCCTGAGAG TACGAGAATG TCAGGTGCTA 
TTTTTAGCCG GCAAAACCAA AGGCTGTTTT TATTCTCCTC CTTACCTTGA TGACTATGGG 
GAGACCGACC AGGGACTCAG ACGGGGAAAT CCTTTACATC TGTGCAAAGA GCGATTTAAG 
AAGATTCAGA AGCTCTGGCA CCAGCACAGT ATCACAGAGG AAATTGGACA TGCGCAGGAA 
GCAAATCAGA CGCTGGTTGG CATTGACTGG CAACATTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006926612.1
CDS	393..5654
Translation	MASELEPEVPAIDRSLLACSAEETAGKWLQATDLTREVYQHLAYYVPKIYCRGPNPLQEEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHKDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHISDVIARTYNIFAIMFRYAVEILTWEKENELPADLEMVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGSIIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAVRFAKNYERLQSDYVTDDHDREFSVADLSVQIFTVPSLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPIILSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIIMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITQKDVVQQNNTLIEEMLYLIIMLVGERFNPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEEVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNKEDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTILQWAVEHNGYTWSESMLQRVLHLIGMALQEEKQHLENVMEEHIVTFTFTQKISKPGEAPNNCPSILAMLETLQNAPYLEVHKDMIRWILKTFNTIKKMRESSSVSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDVSTSAALDNSPMISDMTFTALGPAQTQVPKQRQFVTCILCQEEQEVKVESRAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPNLSCGTHTSSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLFPPRNISNNRLNFSDQPNLTQWIRTISQQIKALQILRKEESIPNIVSSKNSENMDELQLPEGFRPDFHPKNPYSDSIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVALLSVVQEHFCRLFASLVPDDNHEDLPCILDIDMFHLLVGLVLAFPALQCQDFSGISLETGDLHIFHLVTMAHIVQILLTSCTEENGMDQETPAGEEELAVLALYKTLRQYTGSALKDIPSGWHLWRNVRAGIMPFLKCSALFFHYLNGVPSPPEIQVSGTSHFEHLCNYLSLPNNLICLFQENDEIMKLLIESWCQNTEVKRYLEGKRDAISYPRESNKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGTLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_006926550.3
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006926550.3

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC GGAGGTGCCG GCCATCGACC GGAGTTTGCT GGCATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCT ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT ACAGGAGGAA 
GACATGCTGG CACAGCATGT TTTGTTGGGA CCAATGGAAT GGTACCTTTG TGGTGAAGAT 
CCTGCATTTG GATTTCCAAA ACTTGAACAA GCAAACAAAC CTTCTCATCT TTGTGGTCGT 
GTTTTTAAAG TAGGAGAACC CACATATTCT TGCAGAGACT GTGCAGTTGA TCCAACTTGT 
GTTTTATGCA TGGAGTGCTT TTTGGGAAGT ATTCACAAAG ACCATCGATA TAGGATGACA 
ACATCAGGAG GTGGAGGTTT CTGTGACTGT GGTGATACTG AAGCTTGGAA AGAGGGTCCT 
TACTGTCAAA AACATGAACT TAACACCTCT GAAATTGAGG AAGAAGAGGA TCCTCTTGTG 
CATATATCAG ATGTGATAGC AAGAACTTAC AACATTTTTG CTATTATGTT TCGGTATGCA 
GTGGAGATAC TAACCTGGGA AAAAGAAAAT GAATTGCCAG CAGATTTAGA GATGGTAGAG 
AAGAGTGACA CCTACTATTG CATGCTTTTT AATGATGAGG TTCATACCTA TGAACAAGTT 
ATTTATACTC TTCAGAAAGC TGTTAACTGT ACACAAAAAG AAGCCATTGG CTTTGCAACT 
ACAGTAGATC GAGACGGACG TAGGTCTGTT CGATATGGAG ACTTCCAGTA TTGTGAACAA 
GCCAAATCAG TAATTGTGAG AAATACCAGT AGACAGACAA AGCCACTCAA AGTTCAAGTT 
ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTGTC TTGGCTGGGA 
AGTATTATTG GATATTCAGA TGGCCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GTGAAAACTC TTCTCTTGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTCATGA GCAGTCTTCT TATGGATTTG 
AAATACAAGA AGCTGTTTGC TGTTCGATTT GCAAAGAACT ATGAGCGTTT GCAGAGTGAT 
TATGTGACAG ATGACCACGA CAGAGAGTTT TCAGTCGCAG ACCTCTCGGT TCAGATATTC 
ACAGTTCCTT CACTTGCTCG GATGCTCATC ACTGAAGAAA ACCTGATGAC CATTATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA ATTTGAAAGA 
TATACTGCTT TACAAGCTTT CAAATTTAGG AGAGTTCAGA GCCTTATTTT AGACCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTGGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAGGTGGGAC AGCATATTGA AATGGAACCA GAGTGGGAAG CAGCCTTCAC GTTGCAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GATTGGTGTG CTTTAGATGA AAAAGTTTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCAGTA TTGATGCAGT GTCATGGCGG TTTTACTGAT 
GGTGAACAGC CAATCATATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCTCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTGCAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTCAGTGAA 
CTTAGCCCAC CTATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTT 
CATGCAGGAA TGTGGAGAAG AAATGGTTTC TCTCTGGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAA TAATGCTTCA GACAGGTGTC 
TCCATGATGG ACCCAAATCA TTTCCTGATG ATAATGCTCA GCCGCTTTGA ACTTTATCAG 
ATTTTCAGTA CTCCAGACTA CGGAAAAAGA TTTAGTTCTG AGATTACCCA GAAGGATGTA 
GTTCAGCAAA ACAATACTTT AATAGAAGAA ATGTTATACC TCATTATAAT GCTTGTTGGA 
GAGAGATTTA ATCCTGGAGT TGGACAGGTA AATGCTACAG ATGAAATTAA GCGAGAGATT 
ATCCATCAGT TGAGCATCAA ACCTATGGCT CATAGTGAAT TGGTAAAATC TTTACCTGAA 
GATGAGAACA AGGAGACTGG CATGGAGAGT GTCATTGAAG AAGTTGCCCA TTTCAAGAAA 
CCTGGATTAA CAGGACGAGG CATGTATGAA CTGAAACCAG AGTGTGCCAA AGAGTTCAAT 
TTGTATTTCT ATCACTTTTC AAGGGCAGAG CAGTCCAAGG CAGAAGAAGC TCAACGGAAA 
TTGAAAAGAC AAAATAAGGA AGATACAGCA CTTCCACCTC CAGTTTTGCC TCCATTCTGC 
CCTCTGTTCG CAAGTCTGGT TAACATTTTG CAGTCAGATG TCATGTTGTG CATCATGAGA 
ACAATACTGC AGTGGGCTGT GGAACATAAT GGATACACCT GGTCTGAGTC CATGCTGCAA 
AGGGTATTAC ATTTAATTGG AATGGCACTA CAAGAAGAAA AACAGCACTT GGAGAATGTC 
ATGGAAGAGC ATATAGTAAC ATTTACCTTC ACTCAGAAGA TCTCAAAACC TGGTGAAGCA 
CCAAACAACT GTCCTAGTAT ACTAGCTATG CTGGAAACAC TGCAAAATGC TCCCTACCTA 
GAAGTCCACA AAGATATGAT TAGATGGATA TTAAAGACTT TTAATACTAT TAAGAAAATG 
AGGGAGAGTT CATCTGTCAG TCCTGTAGCA GAGACAGAAG GAACCATAAT GGAAGAGAGT 
TCAAGAGACA AAGACAAAGC TGAGAGGAAG AGAAAAGCTG AGATTGCCAG ACTGCGCAGA 
GAAAAGATCA TGGCCCAGAT GTCTGAGATG CAGCGGCACT TCATTGATGA GAACAAAGAA 
CTCTTCCAGC AGACATTAGA ACTGGATGTC TCCACCTCTG CTGCTCTTGA TAATAGCCCT 
ATGATTTCAG ATATGACATT TACAGCATTG GGCCCAGCAC AAACTCAGGT CCCTAAACAA 
AGACAATTTG TTACCTGTAT ATTGTGTCAA GAGGAACAAG AGGTTAAGGT GGAAAGCAGG 
GCAATGGTCC TGGCAGCATT TGTTCAAAGA TCAACTGTAT TATCAAAAAA CAGAAGTAAA 
TTCATTCAGG ATCCAGAAAA ATATGATCCA TTATTCATGC ACCCTAATCT GTCTTGTGGA 
ACACACACCA GTAGCTGTGG ACACATTATG CATGCCCATT GTTGGCAAAG GTATTTTGAT 
TCCGTTCAAG CTAAAGAACA GCGAAGGCAA CAGAGATTAC GCTTACATAC AAGCTATGAC 
GTAGAAAATG GAGAATTCCT GTGTCCCCTT TGTGAATGTT TGAGTAACAC TGTTATTCCT 
CTGCTGTTTC CTCCAAGAAA TATTTCTAAC AACAGGTTAA ATTTTTCAGA CCAACCAAAT 
CTGACTCAGT GGATTAGAAC AATATCTCAG CAAATAAAAG CATTGCAGAT TCTTAGGAAA 
GAAGAAAGTA TTCCTAATAT TGTCTCTTCA AAGAATTCAG AAAATATGGA TGAATTGCAG 
CTCCCTGAAG GATTTAGGCC TGATTTTCAT CCTAAGAACC CTTATTCTGA TAGCATAAAA 
GAAATGCTAA CAACGTTTGG AACTGCTACT TACAAGGTGG GACTAAAGGT TCATCCCAAT 
GAAGAAGATC CCCGCGTGCC CATAATGTGT TGGGGTAGTT GTGCGTACAC CATCCAAAGC 
ATAGAAAGAA TCTTAAGTGA TGAAGATAAA CCGTTGTTTG GTCCTTTGCC TTGCAGACTG 
GATGACTGTC TTAGGTCACT AACAAGGTTC GCTGCAGCAC ACTGGACAGT GGCATTACTC 
TCAGTGGTGC AGGAACATTT TTGTAGACTT TTTGCATCAT TGGTGCCTGA TGACAATCAT 
GAAGACCTTC CATGCATATT AGATATCGAC ATGTTTCATT TATTGGTGGG CTTGGTGCTC 
GCTTTCCCCG CGTTGCAGTG TCAGGATTTT TCAGGGATCA GCCTTGAAAC TGGAGACCTT 
CACATTTTCC ATCTGGTTAC TATGGCACAC ATTGTACAGA TCTTACTTAC CTCGTGTACA 
GAAGAGAATG GCATGGATCA AGAAACTCCT GCTGGTGAAG AAGAATTAGC AGTCCTTGCT 
TTGTATAAAA CACTTCGCCA GTACACAGGA AGTGCCTTGA AAGACATACC CTCTGGCTGG 
CATCTGTGGA GGAATGTCAG AGCTGGAATC ATGCCTTTCC TAAAGTGTTC TGCTTTGTTT 
TTTCATTACT TAAATGGAGT TCCTTCTCCA CCTGAAATTC AAGTTTCTGG AACAAGCCAT 
TTTGAACATT TATGTAACTA TCTTTCCCTG CCAAACAACC TCATTTGTCT TTTTCAAGAA 
AATGATGAGA TAATGAAATT ACTGATTGAA AGTTGGTGTC AGAACACTGA AGTTAAAAGA 
TATCTAGAAG GTAAAAGAGA TGCTATCAGC TATCCAAGAG AATCTAACAA ATTAATAGAC 
CTTCCAGAGG ATTACAGCAG CCTCATTAAT CAAGCATCTA ATTTCTCGTG CCCCAAATCA 
GGTGGTGACA AGAGCAGAGC CCCAACTCTG TGCCTTGTGT GTGGAACTCT GCTGTGCTCC 
CAGAGCTACT GCTGTCAGAC CGAACTGGAA GGGGAGGATG TAGGAGCTTG CACAGCTCAC 
ACCTACTCCT GTGGTTCTGG GGTGGGCATC TTCCTGAGAG TACGAGAATG TCAGGTGCTA 
TTTTTAGCCG GCAAAACCAA AGGCTGTTTT TATTCTCCTC CTTACCTTGA TGACTATGGG 
GAGACCGACC AGGGACTCAG ACGGGGAAAT CCTTTACATC TGTGCAAAGA GCGATTTAAG 
AAGATTCAGA AGCTCTGGCA CCAGCACAGT ATCACAGAGG AAATTGGACA TGCGCAGGAA 
GCAAATCAGA CGCTGGTTGG CATTGACTGG CAACATTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_015600427.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_015455913.1 Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X3, mRNA.

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CloneID	OPt31993
Clone ID Related Accession (Same CDS sequence)	XM_015600427.1 , XM_015600427.2
Accession Version	XM_015600427.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	E3 ubiquitin-protein ligase UBR2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC GGAGGTGCCG GCCATCGACC GGAGTTTGCT GGCATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCT ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT ACAGGAGGAA 
GACATGCTGG CACAGCATGT TTTGTTGGGA CCAATGGAAT GGTACCTTTG TGGTGAAGAT 
CCTGCATTTG GATTTCCAAA ACTTGAACAA GCAAACAAAC CTTCTCATCT TTGTGGTCGT 
GTTTTTAAAG TAGGAGAACC CACATATTCT TGCAGAGACT GTGCAGTTGA TCCAACTTGT 
GTTTTATGCA TGGAGTGCTT TTTGGGAAGT ATTCACAAAG ACCATCGATA TAGGATGACA 
ACATCAGGAG GTGGAGGTTT CTGTGACTGT GGTGATACTG AAGCTTGGAA AGAGGGTCCT 
TACTGTCAAA AACATGAACT TAACACCTCT GAAATTGAGG AAGAAGAGGA TCCTCTTGTG 
CATATATCAG ATGTGATAGC AAGAACTTAC AACATTTTTG CTATTATGTT TCGGTATGCA 
GTGGAGATAC TAACCTGGGA AAAAGAAAAT GAATTGCCAG CAGATTTAGA GATGGTAGAG 
AAGAGTGACA CCTACTATTG CATGCTTTTT AATGATGAGG TTCATACCTA TGAACAAGTT 
ATTTATACTC TTCAGAAAGC TGTTAACTGT ACACAAAAAG AAGCCATTGG CTTTGCAACT 
ACAGTAGATC GAGACGGACG TAGGTCTGTT CGATATGGAG ACTTCCAGTA TTGTGAACAA 
GCCAAATCAG TAATTGTGAG AAATACCAGT AGACAGACAA AGCCACTCAA AGTTCAAGTT 
ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTGTC TTGGCTGGGA 
AGTATTATTG GATATTCAGA TGGCCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GTGAAAACTC TTCTCTTGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTCATGA GCAGTCTTCT TATGGATTTG 
AAATACAAGA AGCTGTTTGC TGTTCGATTT GCAAAGAACT ATGAGCGTTT GCAGAGTGAT 
TATGTGACAG ATGACCACGA CAGAGAGTTT TCAGTCGCAG ACCTCTCGGT TCAGATATTC 
ACAGTTCCTT CACTTGCTCG GATGCTCATC ACTGAAGAAA ACCTGATGAC CATTATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA ATTTGAAAGA 
TATACTGCTT TACAAGCTTT CAAATTTAGG AGAGTTCAGA GCCTTATTTT AGACCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTGGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAGGTGGGAC AGCATATTGA AATGGAACCA GAGTGGGAAG CAGCCTTCAC GTTGCAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GATTGGTGTG CTTTAGATGA AAAAGTTTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCAGTA TTGATGCAGT GTCATGGCGG TTTTACTGAT 
GGTGAACAGC CAATCATATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCTCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTGCAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTCAGTGAA 
CTTAGCCCAC CTATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTT 
CATGCAGGAA TGTGGAGAAG AAATGGTTTC TCTCTGGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAA TAATGCTTCA GACAGGTGTC 
TCCATGATGG ACCCAAATCA TTTCCTGATG ATAATGCTCA GCCGCTTTGA ACTTTATCAG 
ATTTTCAGTA CTCCAGACTA CGGAAAAAGA TTTAGTTCTG AGATTACCCA GAAGGATGTA 
GTTCAGCAAA ACAATACTTT AATAGAAGAA ATGTTATACC TCATTATAAT GCTTGTTGGA 
GAGAGATTTA ATCCTGGAGT TGGACAGGTA AATGCTACAG ATGAAATTAA GCGAGAGATT 
ATCCATCAGT TGAGCATCAA ACCTATGGCT CATAGTGAAT TGGTAAAATC TTTACCTGAA 
GATGAGAACA AGGAGACTGG CATGGAGAGT GTCATTGAAG AAGTTGCCCA TTTCAAGAAA 
CCTGGATTAA CAGGACGAGG CATGTATGAA CTGAAACCAG AGTGTGCCAA AGAGTTCAAT 
TTGTATTTCT ATCACTTTTC AAGGGCAGAG CAGTCCAAGG CAGAAGAAGC TCAACGGAAA 
TTGAAAAGAC AAAATAAGGA AGATACAGCA CTTCCACCTC CAGTTTTGCC TCCATTCTGC 
CCTCTGTTCG CAAGTCTGGT TAACATTTTG CAGTCAGATG TCATGTTGTG CATCATGAGA 
ACAATACTGC AGTGGGCTGT GGAACATAAT GGATACACCT GGTCTGAGTC CATGCTGCAA 
AGGGTATTAC ATTTAATTGG AATGGCACTA CAAGAAGAAA AACAGCACTT GGAGAATGTC 
ATGGAAGAGC ATATAGTAAC ATTTACCTTC ACTCAGAAGA TCTCAAAACC TGGTGAAGCA 
CCAAACAACT GTCCTAGTAT ACTAGCTATG CTGGAAACAC TGCAAAATGC TCCCTACCTA 
GAAGTCCACA AAGATATGAT TAGATGGATA TTAAAGACTT TTAATACTAT TAAGAAAATG 
AGGGAGAGTT CATCTGTCAG TCCTGTAGCA GAGACAGAAG GAACCATAAT GGAAGAGAGT 
TCAAGAGACA AAGACAAAGC TGAGAGGAAG AGAAAAGCTG AGATTGCCAG ACTGCGCAGA 
GAAAAGATCA TGGCCCAGAT GTCTGAGATG CAGCGGCACT TCATTGATGA GAACAAAGAA 
CTCTTCCAGC AGACATTAGA ACTGGATGTC TCCACCTCTG CTGCTCTTGA TAATAGCCCT 
ATGATTTCAG ATATGACATT TACAGCATTG GGCCCAGCAC AAACTCAGGT CCCTAAACAA 
AGACAATTTG TTACCTGTAT ATTGTGTCAA GAGGAACAAG AGGTTAAGGT GGAAAGCAGG 
GCAATGGTCC TGGCAGCATT TGTTCAAAGA TCAACTGTAT TATCAAAAAA CAGAAGTAAA 
TTCATTCAGG ATCCAGAAAA ATATGATCCA TTATTCATGC ACCCTAATCT GTCTTGTGGA 
ACACACACCA GTAGCTGTGG ACACATTATG CATGCCCATT GTTGGCAAAG GTATTTTGAT 
TCCGTTCAAG CTAAAGAACA GCGAAGGCAA CAGAGATTAC GCTTACATAC AAGCTATGAC 
GTAGAAAATG GAGAATTCCT GTGTCCCCTT TGTGAATGTT TGAGTAACAC TGTTATTCCT 
CTGCTGTTTC CTCCAAGAAA TATTTCTAAC AAGTTAAATT TTTCAGACCA ACCAAATCTG 
ACTCAGTGGA TTAGAACAAT ATCTCAGCAA ATAAAAGCAT TGCAGATTCT TAGGAAAGAA 
GAAAGTATTC CTAATATTGT CTCTTCAAAG AATTCAGAAA ATATGGATGA ATTGCAGCTC 
CCTGAAGGAT TTAGGCCTGA TTTTCATCCT AAGAACCCTT ATTCTGATAG CATAAAAGAA 
ATGCTAACAA CGTTTGGAAC TGCTACTTAC AAGGTGGGAC TAAAGGTTCA TCCCAATGAA 
GAAGATCCCC GCGTGCCCAT AATGTGTTGG GGTAGTTGTG CGTACACCAT CCAAAGCATA 
GAAAGAATCT TAAGTGATGA AGATAAACCG TTGTTTGGTC CTTTGCCTTG CAGACTGGAT 
GACTGTCTTA GGTCACTAAC AAGGTTCGCT GCAGCACACT GGACAGTGGC ATTACTCTCA 
GTGGTGCAGG AACATTTTTG TAGACTTTTT GCATCATTGG TGCCTGATGA CAATCATGAA 
GACCTTCCAT GCATATTAGA TATCGACATG TTTCATTTAT TGGTGGGCTT GGTGCTCGCT 
TTCCCCGCGT TGCAGTGTCA GGATTTTTCA GGGATCAGCC TTGAAACTGG AGACCTTCAC 
ATTTTCCATC TGGTTACTAT GGCACACATT GTACAGATCT TACTTACCTC GTGTACAGAA 
GAGAATGGCA TGGATCAAGA AACTCCTGCT GGTGAAGAAG AATTAGCAGT CCTTGCTTTG 
TATAAAACAC TTCGCCAGTA CACAGGAAGT GCCTTGAAAG ACATACCCTC TGGCTGGCAT 
CTGTGGAGGA ATGTCAGAGC TGGAATCATG CCTTTCCTAA AGTGTTCTGC TTTGTTTTTT 
CATTACTTAA ATGGAGTTCC TTCTCCACCT GAAATTCAAG TTTCTGGAAC AAGCCATTTT 
GAACATTTAT GTAACTATCT TTCCCTGCCA AACAACCTCA TTTGTCTTTT TCAAGAAAAT 
GATGAGATAA TGAAATTACT GATTGAAAGT TGGTGTCAGA ACACTGAAGT TAAAAGATAT 
CTAGAAGGTA AAAGAGATGC TATCAGCTAT CCAAGAGAAT CTAACAAATT AATAGACCTT 
CCAGAGGATT ACAGCAGCCT CATTAATCAA GCATCTAATT TCTCGTGCCC CAAATCAGGT 
GGTGACAAGA GCAGAGCCCC AACTCTGTGC CTTGTGTGTG GAACTCTGCT GTGCTCCCAG 
AGCTACTGCT GTCAGACCGA ACTGGAAGGG GAGGATGTAG GAGCTTGCAC AGCTCACACC 
TACTCCTGTG GTTCTGGGGT GGGCATCTTC CTGAGAGTAC GAGAATGTCA GGTGCTATTT 
TTAGCCGGCA AAACCAAAGG CTGTTTTTAT TCTCCTCCTT ACCTTGATGA CTATGGGGAG 
ACCGACCAGG GACTCAGACG GGGAAATCCT TTACATCTGT GCAAAGAGCG ATTTAAGAAG 
ATTCAGAAGC TCTGGCACCA GCACAGTATC ACAGAGGAAA TTGGACATGC GCAGGAAGCA 
AATCAGACGC TGGTTGGCAT TGACTGGCAA CATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015455913.1
CDS	365..5623
Translation	MASELEPEVPAIDRSLLACSAEETAGKWLQATDLTREVYQHLAYYVPKIYCRGPNPLQEEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHKDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHISDVIARTYNIFAIMFRYAVEILTWEKENELPADLEMVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGSIIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAVRFAKNYERLQSDYVTDDHDREFSVADLSVQIFTVPSLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPIILSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIIMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITQKDVVQQNNTLIEEMLYLIIMLVGERFNPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEEVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNKEDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTILQWAVEHNGYTWSESMLQRVLHLIGMALQEEKQHLENVMEEHIVTFTFTQKISKPGEAPNNCPSILAMLETLQNAPYLEVHKDMIRWILKTFNTIKKMRESSSVSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDVSTSAALDNSPMISDMTFTALGPAQTQVPKQRQFVTCILCQEEQEVKVESRAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPNLSCGTHTSSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLFPPRNISNKLNFSDQPNLTQWIRTISQQIKALQILRKEESIPNIVSSKNSENMDELQLPEGFRPDFHPKNPYSDSIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVALLSVVQEHFCRLFASLVPDDNHEDLPCILDIDMFHLLVGLVLAFPALQCQDFSGISLETGDLHIFHLVTMAHIVQILLTSCTEENGMDQETPAGEEELAVLALYKTLRQYTGSALKDIPSGWHLWRNVRAGIMPFLKCSALFFHYLNGVPSPPEIQVSGTSHFEHLCNYLSLPNNLICLFQENDEIMKLLIESWCQNTEVKRYLEGKRDAISYPRESNKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGTLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_015600427.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015600427.1

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC GGAGGTGCCG GCCATCGACC GGAGTTTGCT GGCATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCT ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT ACAGGAGGAA 
GACATGCTGG CACAGCATGT TTTGTTGGGA CCAATGGAAT GGTACCTTTG TGGTGAAGAT 
CCTGCATTTG GATTTCCAAA ACTTGAACAA GCAAACAAAC CTTCTCATCT TTGTGGTCGT 
GTTTTTAAAG TAGGAGAACC CACATATTCT TGCAGAGACT GTGCAGTTGA TCCAACTTGT 
GTTTTATGCA TGGAGTGCTT TTTGGGAAGT ATTCACAAAG ACCATCGATA TAGGATGACA 
ACATCAGGAG GTGGAGGTTT CTGTGACTGT GGTGATACTG AAGCTTGGAA AGAGGGTCCT 
TACTGTCAAA AACATGAACT TAACACCTCT GAAATTGAGG AAGAAGAGGA TCCTCTTGTG 
CATATATCAG ATGTGATAGC AAGAACTTAC AACATTTTTG CTATTATGTT TCGGTATGCA 
GTGGAGATAC TAACCTGGGA AAAAGAAAAT GAATTGCCAG CAGATTTAGA GATGGTAGAG 
AAGAGTGACA CCTACTATTG CATGCTTTTT AATGATGAGG TTCATACCTA TGAACAAGTT 
ATTTATACTC TTCAGAAAGC TGTTAACTGT ACACAAAAAG AAGCCATTGG CTTTGCAACT 
ACAGTAGATC GAGACGGACG TAGGTCTGTT CGATATGGAG ACTTCCAGTA TTGTGAACAA 
GCCAAATCAG TAATTGTGAG AAATACCAGT AGACAGACAA AGCCACTCAA AGTTCAAGTT 
ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTGTC TTGGCTGGGA 
AGTATTATTG GATATTCAGA TGGCCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GTGAAAACTC TTCTCTTGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTCATGA GCAGTCTTCT TATGGATTTG 
AAATACAAGA AGCTGTTTGC TGTTCGATTT GCAAAGAACT ATGAGCGTTT GCAGAGTGAT 
TATGTGACAG ATGACCACGA CAGAGAGTTT TCAGTCGCAG ACCTCTCGGT TCAGATATTC 
ACAGTTCCTT CACTTGCTCG GATGCTCATC ACTGAAGAAA ACCTGATGAC CATTATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA ATTTGAAAGA 
TATACTGCTT TACAAGCTTT CAAATTTAGG AGAGTTCAGA GCCTTATTTT AGACCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTGGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAGGTGGGAC AGCATATTGA AATGGAACCA GAGTGGGAAG CAGCCTTCAC GTTGCAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GATTGGTGTG CTTTAGATGA AAAAGTTTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCAGTA TTGATGCAGT GTCATGGCGG TTTTACTGAT 
GGTGAACAGC CAATCATATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCTCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTGCAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTCAGTGAA 
CTTAGCCCAC CTATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTT 
CATGCAGGAA TGTGGAGAAG AAATGGTTTC TCTCTGGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAA TAATGCTTCA GACAGGTGTC 
TCCATGATGG ACCCAAATCA TTTCCTGATG ATAATGCTCA GCCGCTTTGA ACTTTATCAG 
ATTTTCAGTA CTCCAGACTA CGGAAAAAGA TTTAGTTCTG AGATTACCCA GAAGGATGTA 
GTTCAGCAAA ACAATACTTT AATAGAAGAA ATGTTATACC TCATTATAAT GCTTGTTGGA 
GAGAGATTTA ATCCTGGAGT TGGACAGGTA AATGCTACAG ATGAAATTAA GCGAGAGATT 
ATCCATCAGT TGAGCATCAA ACCTATGGCT CATAGTGAAT TGGTAAAATC TTTACCTGAA 
GATGAGAACA AGGAGACTGG CATGGAGAGT GTCATTGAAG AAGTTGCCCA TTTCAAGAAA 
CCTGGATTAA CAGGACGAGG CATGTATGAA CTGAAACCAG AGTGTGCCAA AGAGTTCAAT 
TTGTATTTCT ATCACTTTTC AAGGGCAGAG CAGTCCAAGG CAGAAGAAGC TCAACGGAAA 
TTGAAAAGAC AAAATAAGGA AGATACAGCA CTTCCACCTC CAGTTTTGCC TCCATTCTGC 
CCTCTGTTCG CAAGTCTGGT TAACATTTTG CAGTCAGATG TCATGTTGTG CATCATGAGA 
ACAATACTGC AGTGGGCTGT GGAACATAAT GGATACACCT GGTCTGAGTC CATGCTGCAA 
AGGGTATTAC ATTTAATTGG AATGGCACTA CAAGAAGAAA AACAGCACTT GGAGAATGTC 
ATGGAAGAGC ATATAGTAAC ATTTACCTTC ACTCAGAAGA TCTCAAAACC TGGTGAAGCA 
CCAAACAACT GTCCTAGTAT ACTAGCTATG CTGGAAACAC TGCAAAATGC TCCCTACCTA 
GAAGTCCACA AAGATATGAT TAGATGGATA TTAAAGACTT TTAATACTAT TAAGAAAATG 
AGGGAGAGTT CATCTGTCAG TCCTGTAGCA GAGACAGAAG GAACCATAAT GGAAGAGAGT 
TCAAGAGACA AAGACAAAGC TGAGAGGAAG AGAAAAGCTG AGATTGCCAG ACTGCGCAGA 
GAAAAGATCA TGGCCCAGAT GTCTGAGATG CAGCGGCACT TCATTGATGA GAACAAAGAA 
CTCTTCCAGC AGACATTAGA ACTGGATGTC TCCACCTCTG CTGCTCTTGA TAATAGCCCT 
ATGATTTCAG ATATGACATT TACAGCATTG GGCCCAGCAC AAACTCAGGT CCCTAAACAA 
AGACAATTTG TTACCTGTAT ATTGTGTCAA GAGGAACAAG AGGTTAAGGT GGAAAGCAGG 
GCAATGGTCC TGGCAGCATT TGTTCAAAGA TCAACTGTAT TATCAAAAAA CAGAAGTAAA 
TTCATTCAGG ATCCAGAAAA ATATGATCCA TTATTCATGC ACCCTAATCT GTCTTGTGGA 
ACACACACCA GTAGCTGTGG ACACATTATG CATGCCCATT GTTGGCAAAG GTATTTTGAT 
TCCGTTCAAG CTAAAGAACA GCGAAGGCAA CAGAGATTAC GCTTACATAC AAGCTATGAC 
GTAGAAAATG GAGAATTCCT GTGTCCCCTT TGTGAATGTT TGAGTAACAC TGTTATTCCT 
CTGCTGTTTC CTCCAAGAAA TATTTCTAAC AAGTTAAATT TTTCAGACCA ACCAAATCTG 
ACTCAGTGGA TTAGAACAAT ATCTCAGCAA ATAAAAGCAT TGCAGATTCT TAGGAAAGAA 
GAAAGTATTC CTAATATTGT CTCTTCAAAG AATTCAGAAA ATATGGATGA ATTGCAGCTC 
CCTGAAGGAT TTAGGCCTGA TTTTCATCCT AAGAACCCTT ATTCTGATAG CATAAAAGAA 
ATGCTAACAA CGTTTGGAAC TGCTACTTAC AAGGTGGGAC TAAAGGTTCA TCCCAATGAA 
GAAGATCCCC GCGTGCCCAT AATGTGTTGG GGTAGTTGTG CGTACACCAT CCAAAGCATA 
GAAAGAATCT TAAGTGATGA AGATAAACCG TTGTTTGGTC CTTTGCCTTG CAGACTGGAT 
GACTGTCTTA GGTCACTAAC AAGGTTCGCT GCAGCACACT GGACAGTGGC ATTACTCTCA 
GTGGTGCAGG AACATTTTTG TAGACTTTTT GCATCATTGG TGCCTGATGA CAATCATGAA 
GACCTTCCAT GCATATTAGA TATCGACATG TTTCATTTAT TGGTGGGCTT GGTGCTCGCT 
TTCCCCGCGT TGCAGTGTCA GGATTTTTCA GGGATCAGCC TTGAAACTGG AGACCTTCAC 
ATTTTCCATC TGGTTACTAT GGCACACATT GTACAGATCT TACTTACCTC GTGTACAGAA 
GAGAATGGCA TGGATCAAGA AACTCCTGCT GGTGAAGAAG AATTAGCAGT CCTTGCTTTG 
TATAAAACAC TTCGCCAGTA CACAGGAAGT GCCTTGAAAG ACATACCCTC TGGCTGGCAT 
CTGTGGAGGA ATGTCAGAGC TGGAATCATG CCTTTCCTAA AGTGTTCTGC TTTGTTTTTT 
CATTACTTAA ATGGAGTTCC TTCTCCACCT GAAATTCAAG TTTCTGGAAC AAGCCATTTT 
GAACATTTAT GTAACTATCT TTCCCTGCCA AACAACCTCA TTTGTCTTTT TCAAGAAAAT 
GATGAGATAA TGAAATTACT GATTGAAAGT TGGTGTCAGA ACACTGAAGT TAAAAGATAT 
CTAGAAGGTA AAAGAGATGC TATCAGCTAT CCAAGAGAAT CTAACAAATT AATAGACCTT 
CCAGAGGATT ACAGCAGCCT CATTAATCAA GCATCTAATT TCTCGTGCCC CAAATCAGGT 
GGTGACAAGA GCAGAGCCCC AACTCTGTGC CTTGTGTGTG GAACTCTGCT GTGCTCCCAG 
AGCTACTGCT GTCAGACCGA ACTGGAAGGG GAGGATGTAG GAGCTTGCAC AGCTCACACC 
TACTCCTGTG GTTCTGGGGT GGGCATCTTC CTGAGAGTAC GAGAATGTCA GGTGCTATTT 
TTAGCCGGCA AAACCAAAGG CTGTTTTTAT TCTCCTCCTT ACCTTGATGA CTATGGGGAG 
ACCGACCAGG GACTCAGACG GGGAAATCCT TTACATCTGT GCAAAGAGCG ATTTAAGAAG 
ATTCAGAAGC TCTGGCACCA GCACAGTATC ACAGAGGAAA TTGGACATGC GCAGGAAGCA 
AATCAGACGC TGGTTGGCAT TGACTGGCAA CATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_015600427.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_015455913.1 Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X3, mRNA.

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CloneID	OPt31993
Clone ID Related Accession (Same CDS sequence)	XM_015600427.1 , XM_015600427.2
Accession Version	XM_015600427.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	E3 ubiquitin-protein ligase UBR2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015600427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCTGGAGCC GGAGGTGCCG GCCATCGACC GGAGTTTGCT GGCATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCT ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT ACAGGAGGAA 
GACATGCTGG CACAGCATGT TTTGTTGGGA CCAATGGAAT GGTACCTTTG TGGTGAAGAT 
CCTGCATTTG GATTTCCAAA ACTTGAACAA GCAAACAAAC CTTCTCATCT TTGTGGTCGT 
GTTTTTAAAG TAGGAGAACC CACATATTCT TGCAGAGACT GTGCAGTTGA TCCAACTTGT 
GTTTTATGCA TGGAGTGCTT TTTGGGAAGT ATTCACAAAG ACCATCGATA TAGGATGACA 
ACATCAGGAG GTGGAGGTTT CTGTGACTGT GGTGATACTG AAGCTTGGAA AGAGGGTCCT 
TACTGTCAAA AACATGAACT TAACACCTCT GAAATTGAGG AAGAAGAGGA TCCTCTTGTG 
CATATATCAG ATGTGATAGC AAGAACTTAC AACATTTTTG CTATTATGTT TCGGTATGCA 
GTGGAGATAC TAACCTGGGA AAAAGAAAAT GAATTGCCAG CAGATTTAGA GATGGTAGAG 
AAGAGTGACA CCTACTATTG CATGCTTTTT AATGATGAGG TTCATACCTA TGAACAAGTT 
ATTTATACTC TTCAGAAAGC TGTTAACTGT ACACAAAAAG AAGCCATTGG CTTTGCAACT 
ACAGTAGATC GAGACGGACG TAGGTCTGTT CGATATGGAG ACTTCCAGTA TTGTGAACAA 
GCCAAATCAG TAATTGTGAG AAATACCAGT AGACAGACAA AGCCACTCAA AGTTCAAGTT 
ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTGTC TTGGCTGGGA 
AGTATTATTG GATATTCAGA TGGCCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GTGAAAACTC TTCTCTTGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTCATGA GCAGTCTTCT TATGGATTTG 
AAATACAAGA AGCTGTTTGC TGTTCGATTT GCAAAGAACT ATGAGCGTTT GCAGAGTGAT 
TATGTGACAG ATGACCACGA CAGAGAGTTT TCAGTCGCAG ACCTCTCGGT TCAGATATTC 
ACAGTTCCTT CACTTGCTCG GATGCTCATC ACTGAAGAAA ACCTGATGAC CATTATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA ATTTGAAAGA 
TATACTGCTT TACAAGCTTT CAAATTTAGG AGAGTTCAGA GCCTTATTTT AGACCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTGGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAGGTGGGAC AGCATATTGA AATGGAACCA GAGTGGGAAG CAGCCTTCAC GTTGCAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GATTGGTGTG CTTTAGATGA AAAAGTTTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCAGTA TTGATGCAGT GTCATGGCGG TTTTACTGAT 
GGTGAACAGC CAATCATATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCTCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTGCAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTCAGTGAA 
CTTAGCCCAC CTATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTT 
CATGCAGGAA TGTGGAGAAG AAATGGTTTC TCTCTGGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAA TAATGCTTCA GACAGGTGTC 
TCCATGATGG ACCCAAATCA TTTCCTGATG ATAATGCTCA GCCGCTTTGA ACTTTATCAG 
ATTTTCAGTA CTCCAGACTA CGGAAAAAGA TTTAGTTCTG AGATTACCCA GAAGGATGTA 
GTTCAGCAAA ACAATACTTT AATAGAAGAA ATGTTATACC TCATTATAAT GCTTGTTGGA 
GAGAGATTTA ATCCTGGAGT TGGACAGGTA AATGCTACAG ATGAAATTAA GCGAGAGATT 
ATCCATCAGT TGAGCATCAA ACCTATGGCT CATAGTGAAT TGGTAAAATC TTTACCTGAA 
GATGAGAACA AGGAGACTGG CATGGAGAGT GTCATTGAAG AAGTTGCCCA TTTCAAGAAA 
CCTGGATTAA CAGGACGAGG CATGTATGAA CTGAAACCAG AGTGTGCCAA AGAGTTCAAT 
TTGTATTTCT ATCACTTTTC AAGGGCAGAG CAGTCCAAGG CAGAAGAAGC TCAACGGAAA 
TTGAAAAGAC AAAATAAGGA AGATACAGCA CTTCCACCTC CAGTTTTGCC TCCATTCTGC 
CCTCTGTTCG CAAGTCTGGT TAACATTTTG CAGTCAGATG TCATGTTGTG CATCATGAGA 
ACAATACTGC AGTGGGCTGT GGAACATAAT GGATACACCT GGTCTGAGTC CATGCTGCAA 
AGGGTATTAC ATTTAATTGG AATGGCACTA CAAGAAGAAA AACAGCACTT GGAGAATGTC 
ATGGAAGAGC ATATAGTAAC ATTTACCTTC ACTCAGAAGA TCTCAAAACC TGGTGAAGCA 
CCAAACAACT GTCCTAGTAT ACTAGCTATG CTGGAAACAC TGCAAAATGC TCCCTACCTA 
GAAGTCCACA AAGATATGAT TAGATGGATA TTAAAGACTT TTAATACTAT TAAGAAAATG 
AGGGAGAGTT CATCTGTCAG TCCTGTAGCA GAGACAGAAG GAACCATAAT GGAAGAGAGT 
TCAAGAGACA AAGACAAAGC TGAGAGGAAG AGAAAAGCTG AGATTGCCAG ACTGCGCAGA 
GAAAAGATCA TGGCCCAGAT GTCTGAGATG CAGCGGCACT TCATTGATGA GAACAAAGAA 
CTCTTCCAGC AGACATTAGA ACTGGATGTC TCCACCTCTG CTGCTCTTGA TAATAGCCCT 
ATGATTTCAG ATATGACATT TACAGCATTG GGCCCAGCAC AAACTCAGGT CCCTAAACAA 
AGACAATTTG TTACCTGTAT ATTGTGTCAA GAGGAACAAG AGGTTAAGGT GGAAAGCAGG 
GCAATGGTCC TGGCAGCATT TGTTCAAAGA TCAACTGTAT TATCAAAAAA CAGAAGTAAA 
TTCATTCAGG ATCCAGAAAA ATATGATCCA TTATTCATGC ACCCTAATCT GTCTTGTGGA 
ACACACACCA GTAGCTGTGG ACACATTATG CATGCCCATT GTTGGCAAAG GTATTTTGAT 
TCCGTTCAAG CTAAAGAACA GCGAAGGCAA CAGAGATTAC GCTTACATAC AAGCTATGAC 
GTAGAAAATG GAGAATTCCT GTGTCCCCTT TGTGAATGTT TGAGTAACAC TGTTATTCCT 
CTGCTGTTTC CTCCAAGAAA TATTTCTAAC AAGTTAAATT TTTCAGACCA ACCAAATCTG 
ACTCAGTGGA TTAGAACAAT ATCTCAGCAA ATAAAAGCAT TGCAGATTCT TAGGAAAGAA 
GAAAGTATTC CTAATATTGT CTCTTCAAAG AATTCAGAAA ATATGGATGA ATTGCAGCTC 
CCTGAAGGAT TTAGGCCTGA TTTTCATCCT AAGAACCCTT ATTCTGATAG CATAAAAGAA 
ATGCTAACAA CGTTTGGAAC TGCTACTTAC AAGGTGGGAC TAAAGGTTCA TCCCAATGAA 
GAAGATCCCC GCGTGCCCAT AATGTGTTGG GGTAGTTGTG CGTACACCAT CCAAAGCATA 
GAAAGAATCT TAAGTGATGA AGATAAACCG TTGTTTGGTC CTTTGCCTTG CAGACTGGAT 
GACTGTCTTA GGTCACTAAC AAGGTTCGCT GCAGCACACT GGACAGTGGC ATTACTCTCA 
GTGGTGCAGG AACATTTTTG TAGACTTTTT GCATCATTGG TGCCTGATGA CAATCATGAA 
GACCTTCCAT GCATATTAGA TATCGACATG TTTCATTTAT TGGTGGGCTT GGTGCTCGCT 
TTCCCCGCGT TGCAGTGTCA GGATTTTTCA GGGATCAGCC TTGAAACTGG AGACCTTCAC 
ATTTTCCATC TGGTTACTAT GGCACACATT GTACAGATCT TACTTACCTC GTGTACAGAA 
GAGAATGGCA TGGATCAAGA AACTCCTGCT GGTGAAGAAG AATTAGCAGT CCTTGCTTTG 
TATAAAACAC TTCGCCAGTA CACAGGAAGT GCCTTGAAAG ACATACCCTC TGGCTGGCAT 
CTGTGGAGGA ATGTCAGAGC TGGAATCATG CCTTTCCTAA AGTGTTCTGC TTTGTTTTTT 
CATTACTTAA ATGGAGTTCC TTCTCCACCT GAAATTCAAG TTTCTGGAAC AAGCCATTTT 
GAACATTTAT GTAACTATCT TTCCCTGCCA AACAACCTCA TTTGTCTTTT TCAAGAAAAT 
GATGAGATAA TGAAATTACT GATTGAAAGT TGGTGTCAGA ACACTGAAGT TAAAAGATAT 
CTAGAAGGTA AAAGAGATGC TATCAGCTAT CCAAGAGAAT CTAACAAATT AATAGACCTT 
CCAGAGGATT ACAGCAGCCT CATTAATCAA GCATCTAATT TCTCGTGCCC CAAATCAGGT 
GGTGACAAGA GCAGAGCCCC AACTCTGTGC CTTGTGTGTG GAACTCTGCT GTGCTCCCAG 
AGCTACTGCT GTCAGACCGA ACTGGAAGGG GAGGATGTAG GAGCTTGCAC AGCTCACACC 
TACTCCTGTG GTTCTGGGGT GGGCATCTTC CTGAGAGTAC GAGAATGTCA GGTGCTATTT 
TTAGCCGGCA AAACCAAAGG CTGTTTTTAT TCTCCTCCTT ACCTTGATGA CTATGGGGAG 
ACCGACCAGG GACTCAGACG GGGAAATCCT TTACATCTGT GCAAAGAGCG ATTTAAGAAG 
ATTCAGAAGC TCTGGCACCA GCACAGTATC ACAGAGGAAA TTGGACATGC GCAGGAAGCA 
AATCAGACGC TGGTTGGCAT TGACTGGCAA CATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015455913.1
CDS	401..5659
Translation	MASELEPEVPAIDRSLLACSAEETAGKWLQATDLTREVYQHLAYYVPKIYCRGPNPLQEEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHKDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHISDVIARTYNIFAIMFRYAVEILTWEKENELPADLEMVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGSIIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAVRFAKNYERLQSDYVTDDHDREFSVADLSVQIFTVPSLARMLITEENLMTIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDDLRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCALDEKVLIEAYKKCLAVLMQCHGGFTDGEQPIILSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIIMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITQKDVVQQNNTLIEEMLYLIIMLVGERFNPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEEVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNKEDTALPPPVLPPFCPLFASLVNILQSDVMLCIMRTILQWAVEHNGYTWSESMLQRVLHLIGMALQEEKQHLENVMEEHIVTFTFTQKISKPGEAPNNCPSILAMLETLQNAPYLEVHKDMIRWILKTFNTIKKMRESSSVSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDVSTSAALDNSPMISDMTFTALGPAQTQVPKQRQFVTCILCQEEQEVKVESRAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPNLSCGTHTSSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLFPPRNISNKLNFSDQPNLTQWIRTISQQIKALQILRKEESIPNIVSSKNSENMDELQLPEGFRPDFHPKNPYSDSIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVALLSVVQEHFCRLFASLVPDDNHEDLPCILDIDMFHLLVGLVLAFPALQCQDFSGISLETGDLHIFHLVTMAHIVQILLTSCTEENGMDQETPAGEEELAVLALYKTLRQYTGSALKDIPSGWHLWRNVRAGIMPFLKCSALFFHYLNGVPSPPEIQVSGTSHFEHLCNYLSLPNNLICLFQENDEIMKLLIESWCQNTEVKRYLEGKRDAISYPRESNKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGTLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_015600427.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015600427.2

ORF Insert Sequence:

ATGGCGTCGG AGCTGGAGCC GGAGGTGCCG GCCATCGACC GGAGTTTGCT GGCATGTTCG 
GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTGTACCAG 
CATTTAGCCT ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT ACAGGAGGAA 
GACATGCTGG CACAGCATGT TTTGTTGGGA CCAATGGAAT GGTACCTTTG TGGTGAAGAT 
CCTGCATTTG GATTTCCAAA ACTTGAACAA GCAAACAAAC CTTCTCATCT TTGTGGTCGT 
GTTTTTAAAG TAGGAGAACC CACATATTCT TGCAGAGACT GTGCAGTTGA TCCAACTTGT 
GTTTTATGCA TGGAGTGCTT TTTGGGAAGT ATTCACAAAG ACCATCGATA TAGGATGACA 
ACATCAGGAG GTGGAGGTTT CTGTGACTGT GGTGATACTG AAGCTTGGAA AGAGGGTCCT 
TACTGTCAAA AACATGAACT TAACACCTCT GAAATTGAGG AAGAAGAGGA TCCTCTTGTG 
CATATATCAG ATGTGATAGC AAGAACTTAC AACATTTTTG CTATTATGTT TCGGTATGCA 
GTGGAGATAC TAACCTGGGA AAAAGAAAAT GAATTGCCAG CAGATTTAGA GATGGTAGAG 
AAGAGTGACA CCTACTATTG CATGCTTTTT AATGATGAGG TTCATACCTA TGAACAAGTT 
ATTTATACTC TTCAGAAAGC TGTTAACTGT ACACAAAAAG AAGCCATTGG CTTTGCAACT 
ACAGTAGATC GAGACGGACG TAGGTCTGTT CGATATGGAG ACTTCCAGTA TTGTGAACAA 
GCCAAATCAG TAATTGTGAG AAATACCAGT AGACAGACAA AGCCACTCAA AGTTCAAGTT 
ATGCATTCGT CTATTGTCGC ACATCAGAAT TTTGGTTTGA AACTTTTGTC TTGGCTGGGA 
AGTATTATTG GATATTCAGA TGGCCTTCGC CGAATTTTAT GTCAAGTTGG TTTACAAGAA 
GGGCCAGATG GTGAAAACTC TTCTCTTGTG GATAGATTGA TGCTTAATGA TTCCAAATTG 
TGGAAAGGTG CTAGGAGTGT GTATCATCAG TTGTTCATGA GCAGTCTTCT TATGGATTTG 
AAATACAAGA AGCTGTTTGC TGTTCGATTT GCAAAGAACT ATGAGCGTTT GCAGAGTGAT 
TATGTGACAG ATGACCACGA CAGAGAGTTT TCAGTCGCAG ACCTCTCGGT TCAGATATTC 
ACAGTTCCTT CACTTGCTCG GATGCTCATC ACTGAAGAAA ACCTGATGAC CATTATCATT 
AAGACTTTTA TGGATCATTT GAGACATCGA GATGCCCAGG GCAGATTTCA ATTTGAAAGA 
TATACTGCTT TACAAGCTTT CAAATTTAGG AGAGTTCAGA GCCTTATTTT AGACCTCAAG 
TATGTGTTAA TTAGCAAACC AACTGAATGG TCAGATGATC TGAGGCAGAA GTTCCTGGAA 
GGATTTGATG CCTTTTTGGA ATTACTCAAA TGTATGCAGG GAATGGATCC AATTACACGT 
CAGGTGGGAC AGCATATTGA AATGGAACCA GAGTGGGAAG CAGCCTTCAC GTTGCAAATG 
AAATTAACAC ATGTCATTTC AATGATGCAG GATTGGTGTG CTTTAGATGA AAAAGTTTTA 
ATTGAAGCTT ACAAGAAATG CCTTGCAGTA TTGATGCAGT GTCATGGCGG TTTTACTGAT 
GGTGAACAGC CAATCATATT AAGCATTTGT GGACATTCAG TGGAAACTAT CAGATACTGT 
GTTTCTCAAG AAAAAGTTAG CATTCACCTC CCAGTTTCTC GCTTACTTGC AGGTTTGCAT 
GTATTATTAA GCAAAAGTGA AGTGGCATAT AAATTTCCAG AGCTCCTACC TCTCAGTGAA 
CTTAGCCCAC CTATGTTGAT AGAACACCCT CTTAGATGTC TTGTTTTATG TGCCCAAGTT 
CATGCAGGAA TGTGGAGAAG AAATGGTTTC TCTCTGGTAA ACCAGATTTA TTACTACCAT 
AATGTGAAAT GCAGACGTGA AATGTTTGAC AAGGATATAA TAATGCTTCA GACAGGTGTC 
TCCATGATGG ACCCAAATCA TTTCCTGATG ATAATGCTCA GCCGCTTTGA ACTTTATCAG 
ATTTTCAGTA CTCCAGACTA CGGAAAAAGA TTTAGTTCTG AGATTACCCA GAAGGATGTA 
GTTCAGCAAA ACAATACTTT AATAGAAGAA ATGTTATACC TCATTATAAT GCTTGTTGGA 
GAGAGATTTA ATCCTGGAGT TGGACAGGTA AATGCTACAG ATGAAATTAA GCGAGAGATT 
ATCCATCAGT TGAGCATCAA ACCTATGGCT CATAGTGAAT TGGTAAAATC TTTACCTGAA 
GATGAGAACA AGGAGACTGG CATGGAGAGT GTCATTGAAG AAGTTGCCCA TTTCAAGAAA 
CCTGGATTAA CAGGACGAGG CATGTATGAA CTGAAACCAG AGTGTGCCAA AGAGTTCAAT 
TTGTATTTCT ATCACTTTTC AAGGGCAGAG CAGTCCAAGG CAGAAGAAGC TCAACGGAAA 
TTGAAAAGAC AAAATAAGGA AGATACAGCA CTTCCACCTC CAGTTTTGCC TCCATTCTGC 
CCTCTGTTCG CAAGTCTGGT TAACATTTTG CAGTCAGATG TCATGTTGTG CATCATGAGA 
ACAATACTGC AGTGGGCTGT GGAACATAAT GGATACACCT GGTCTGAGTC CATGCTGCAA 
AGGGTATTAC ATTTAATTGG AATGGCACTA CAAGAAGAAA AACAGCACTT GGAGAATGTC 
ATGGAAGAGC ATATAGTAAC ATTTACCTTC ACTCAGAAGA TCTCAAAACC TGGTGAAGCA 
CCAAACAACT GTCCTAGTAT ACTAGCTATG CTGGAAACAC TGCAAAATGC TCCCTACCTA 
GAAGTCCACA AAGATATGAT TAGATGGATA TTAAAGACTT TTAATACTAT TAAGAAAATG 
AGGGAGAGTT CATCTGTCAG TCCTGTAGCA GAGACAGAAG GAACCATAAT GGAAGAGAGT 
TCAAGAGACA AAGACAAAGC TGAGAGGAAG AGAAAAGCTG AGATTGCCAG ACTGCGCAGA 
GAAAAGATCA TGGCCCAGAT GTCTGAGATG CAGCGGCACT TCATTGATGA GAACAAAGAA 
CTCTTCCAGC AGACATTAGA ACTGGATGTC TCCACCTCTG CTGCTCTTGA TAATAGCCCT 
ATGATTTCAG ATATGACATT TACAGCATTG GGCCCAGCAC AAACTCAGGT CCCTAAACAA 
AGACAATTTG TTACCTGTAT ATTGTGTCAA GAGGAACAAG AGGTTAAGGT GGAAAGCAGG 
GCAATGGTCC TGGCAGCATT TGTTCAAAGA TCAACTGTAT TATCAAAAAA CAGAAGTAAA 
TTCATTCAGG ATCCAGAAAA ATATGATCCA TTATTCATGC ACCCTAATCT GTCTTGTGGA 
ACACACACCA GTAGCTGTGG ACACATTATG CATGCCCATT GTTGGCAAAG GTATTTTGAT 
TCCGTTCAAG CTAAAGAACA GCGAAGGCAA CAGAGATTAC GCTTACATAC AAGCTATGAC 
GTAGAAAATG GAGAATTCCT GTGTCCCCTT TGTGAATGTT TGAGTAACAC TGTTATTCCT 
CTGCTGTTTC CTCCAAGAAA TATTTCTAAC AAGTTAAATT TTTCAGACCA ACCAAATCTG 
ACTCAGTGGA TTAGAACAAT ATCTCAGCAA ATAAAAGCAT TGCAGATTCT TAGGAAAGAA 
GAAAGTATTC CTAATATTGT CTCTTCAAAG AATTCAGAAA ATATGGATGA ATTGCAGCTC 
CCTGAAGGAT TTAGGCCTGA TTTTCATCCT AAGAACCCTT ATTCTGATAG CATAAAAGAA 
ATGCTAACAA CGTTTGGAAC TGCTACTTAC AAGGTGGGAC TAAAGGTTCA TCCCAATGAA 
GAAGATCCCC GCGTGCCCAT AATGTGTTGG GGTAGTTGTG CGTACACCAT CCAAAGCATA 
GAAAGAATCT TAAGTGATGA AGATAAACCG TTGTTTGGTC CTTTGCCTTG CAGACTGGAT 
GACTGTCTTA GGTCACTAAC AAGGTTCGCT GCAGCACACT GGACAGTGGC ATTACTCTCA 
GTGGTGCAGG AACATTTTTG TAGACTTTTT GCATCATTGG TGCCTGATGA CAATCATGAA 
GACCTTCCAT GCATATTAGA TATCGACATG TTTCATTTAT TGGTGGGCTT GGTGCTCGCT 
TTCCCCGCGT TGCAGTGTCA GGATTTTTCA GGGATCAGCC TTGAAACTGG AGACCTTCAC 
ATTTTCCATC TGGTTACTAT GGCACACATT GTACAGATCT TACTTACCTC GTGTACAGAA 
GAGAATGGCA TGGATCAAGA AACTCCTGCT GGTGAAGAAG AATTAGCAGT CCTTGCTTTG 
TATAAAACAC TTCGCCAGTA CACAGGAAGT GCCTTGAAAG ACATACCCTC TGGCTGGCAT 
CTGTGGAGGA ATGTCAGAGC TGGAATCATG CCTTTCCTAA AGTGTTCTGC TTTGTTTTTT 
CATTACTTAA ATGGAGTTCC TTCTCCACCT GAAATTCAAG TTTCTGGAAC AAGCCATTTT 
GAACATTTAT GTAACTATCT TTCCCTGCCA AACAACCTCA TTTGTCTTTT TCAAGAAAAT 
GATGAGATAA TGAAATTACT GATTGAAAGT TGGTGTCAGA ACACTGAAGT TAAAAGATAT 
CTAGAAGGTA AAAGAGATGC TATCAGCTAT CCAAGAGAAT CTAACAAATT AATAGACCTT 
CCAGAGGATT ACAGCAGCCT CATTAATCAA GCATCTAATT TCTCGTGCCC CAAATCAGGT 
GGTGACAAGA GCAGAGCCCC AACTCTGTGC CTTGTGTGTG GAACTCTGCT GTGCTCCCAG 
AGCTACTGCT GTCAGACCGA ACTGGAAGGG GAGGATGTAG GAGCTTGCAC AGCTCACACC 
TACTCCTGTG GTTCTGGGGT GGGCATCTTC CTGAGAGTAC GAGAATGTCA GGTGCTATTT 
TTAGCCGGCA AAACCAAAGG CTGTTTTTAT TCTCCTCCTT ACCTTGATGA CTATGGGGAG 
ACCGACCAGG GACTCAGACG GGGAAATCCT TTACATCTGT GCAAAGAGCG ATTTAAGAAG 
ATTCAGAAGC TCTGGCACCA GCACAGTATC ACAGAGGAAA TTGGACATGC GCAGGAAGCA 
AATCAGACGC TGGTTGGCAT TGACTGGCAA CATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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UBR2 cDNA ORF clone, Pteropus alecto(black flying fox)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム