AP4E1 cDNA ORF clone, Pteropus alecto(black flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	AP4E1
Entrez Gene ID	102878591
Full Name	adaptor related protein complex 4 epsilon 1 subunit
Gene Type	protein-coding
Organism	Pteropus alecto(black flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025038750.1	XP_024894518.1	AP-4 complex subunit epsilon-1
XM_015595840.1	XP_015451326.1	AP-4 complex subunit epsilon-1

Pathways from BioSystems
Gene Ontology

The following AP4E1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AP4E1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPt24547	XM_015595840.1 Latest version!	Pteropus alecto adaptor related protein complex 4 epsilon 1 subunit (AP4E1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPt81997	XM_025038750.1 Latest version!	Pteropus alecto adaptor related protein complex 4 subunit epsilon 1 (AP4E1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AP4E1 ( XM_015595840.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_015451326.1 Pteropus alecto adaptor related protein complex 4 epsilon 1 subunit (AP4E1), mRNA.

Price & Availability↑

CloneID	OPt24547
Clone ID Related Accession (Same CDS sequence)	XM_015595840.1
Accession Version	XM_015595840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	AP-4 complex subunit epsilon-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006442109.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGAGCGACA TAGTGGAGAA GACGCTGACG GCCCTGCCGG GACTCTTCCT GCAGAACCAG 
GGGCTTGGTG GGCCCGCGGC CGGCAAGGCG TCTTTCTCTT CACGACTGGG CGGCCTCGTC 
CGCGGCATCA CCGCGCTCAC CTCCAAGCAC GTAGCAACTG TTTCTGCTCC TACAACAACT 
CTGAAAATGA TGAAGGAATG TATGGTGAGA CTTATATATT GTGAAATGCT TGGATATGAT 
GCTTCCTTTG GCTACATACA TGCAATCAAG TTGGCACAAC AAGGAAACCT TTTAGAAAAA 
AGAGTAGGTT ATTTGGCAGT TTCTTTATTT CTACATGAAA GTCATGAACT GTTGCTTCTC 
CTTGTGAATA CGGTCGTAAA GGACTTGCAG AGCACTAACC TGGTAGAAGT ATGTATGGCA 
CTTACTGTCG TCAGCCAGAT TTTCCCTCGA GAAATGATTC CAGCTGTTCT TCCATTAATA 
GAAGATAAAC TTCAACATTC TAAGGAGATT ATACGAAGAA AAGCTGTTTT AGCATTATAC 
AAATTCCATC TCATTGCTCC TAATCAAGTA CAGCATATTC ATATAAAGTT TCAGAAAGCA 
CTTTGTGACA GAGATGTTGG GGTCATGGCT GCTTCCTTGC ACATATACCT TAAGATGATT 
AAGGAGAATT CATCTGGATA TAAAGACTTG ACTGGGAGTT TTGTAACAAT TTTGAAGCAA 
GTAGTTGGAG GAAAGCTCCC AGTAGATTTC AATTACCACA GTGTGCCAGC ACCATGGTTA 
CAAATTCAGC TCTTGAGAAT ACTAGGACTT CTAGGAAAAG ATGATCAAAG GACAAGTGAA 
TTAATGTATG ATGTTCTTGA TGAATCCTTA CGAAGAGCCG AGTTAAATCA CAATGTCACA 
TATGCTATTT TGTTTGAATG TGTGCATACA GTCTATTCTA TTTATCCTAA ATCAGAATTA 
CTTGAGAAGG CTGCCAAGTG CATTGGGAAA TTTGTTCTAT CACCTAAAAT AAATCTCAAA 
TATTTAGGAC TGAAAGCTCT TACCTATGTT ATCCAACAGG ATCCTACTCT GGCTCTTCAA 
CACCAGATGA CAATAATTGA ATGCTTAGAC CATCCTGATC CCATTATTAA GAGAGAGACT 
CTGGAACTTC TTTACAGAAT TACTAATGCA CAGAATGTAA CAGTGATTGT CCAGAAAATG 
CTTGAATATT TACATCAGAG CAAAGAAGAG TATATTATCA TCAATTTGGT TGGCAAAATT 
GCTGAGCTGG CAGAGAAATA TGCTCCTGAT AATGCGTGGT TTATTCAGAC AATGAATGCT 
GTCTTTTCAG TGGGAGGAGA TGTAATGCAT CCTGATATTC CCAATAACTT CCTGAGACTG 
CTAGCAGAAG GTTTTGATGA TGAAACAGAA GATCAACAAT TAAGACTTTA TGCAGTTCAG 
TCTTACCTCA CGTTACTAGA TGTGGAAAAT GTGTTCTATC CACAGAGATT TCTTCAAGTT 
ATGAGTTGGG TGTTAGGGGA GTATTCCTAC CTCTTAGATA AGGAAACACC AGAAGAAGTT 
ATAACCAAGC TCTACAAGTT ACTTATGAAC GACACCACTT CTTCAGAAAC AAAAGCCTGG 
TTAATTGCTG CTGTGACCAA GTTAACCCCC CAAGCACACT CTTCTAGCAT TGTTGAGAGT 
TTAATCCAGG AATTTACCGT GTCTTTGGAT ACTTGTATGA GACAGCATGC ATTTGAATTA 
AAACATTTGC ATGAGAATGT GGAATTTATG AAGAGCTTGC TTCCAGTTAA TAAGAGTTGT 
GAAGACATGG TGGTAGATGC CTCCTTATCT TTTCTGGATG GTTACGTGGC CGAAGAACTC 
AGTCAGGGGG CAGCACCTTA CAAACCTCAC CACCAAAGGC AGGAAGAAAA ACTTTCTCAG 
GAAAAAGTTC TCAATTTTGA ACCGTATGGA CTCTCCTTTT CTTCATCTGG CTTCACTGGA 
CGACAGTCTC CTGCTGGCAT TTCTCTTGGT TCAGATATAT CTGGGAATAG TGCAGAGACA 
GGGCTGAAAG AGACAAATAG CTTGAAGCTG GAAGGTATAA AGAAATTGTG GGGGAAAGAG 
GGCTATCTTC CCAAGAAGGA AAGCAAAACT GGTGATGAAA CTGAAGCTCC GCCTGTTTCT 
CAAGAGAGTA TAATGTTGGA GAATGTAGAT CAAACCACAG CCAAAAAAGA TCAATCTCAA 
GTCCTCACCC AATCTAAAGA GGAGAAAGAA AAGCAGCTGC TGGCATCATC ATTATTTGTG 
GGGCTAGGAT CAGAAAGTAC AATCAATTTG CTGGGAAAAG CAGATAACGT CTCTCACAAG 
TTCAAAAGAA AATCAAAAGT CAAGGAAACC AAAACTGAAG AAACAACCAG TGCTTATAAT 
ACGACCTGTC CTTCCTTTAG TTCTTTATCA AGTGTGACTT ATGAAGATTA TTATTTGGAT 
AATTTGCAGG ATAGAGGAGA CAAAGAATTA AATAAATTTA CTCTCAGTTC AGAACTTTTG 
GATTCTGAAT CACTCACTGA ACTGCCCTTA GTTGAGAAAC TTTCAAATTG CAGGCTATCT 
AAACCTTCAT TGTTTGCGGA TAACAACATG GAAGTTTTTC ACCCTCCTCA ATTCACTGCA 
GCCTCAGTTG GCAATGAAAT CTCTTTAGAT TCCTGTTTGT TGGAAGAAAC TGCTGAACAC 
ATGCATTCAG ATCCTATAGA TGTCTGTAAT GATGAAAACA TGTCGGTGTC TTCTTACAAA 
ATTTGGAAAG ATGATTGTTT ATTGATGGTC TGGTCAGTTT CTAATAAGGG TAGTTCAGAA 
TTGAAAAGTG CTAACTTAGA AATTGCTCCT GCAGAAAATT TCAAGATCAC CGAGCAACCT 
GGATGCTGCT TGCCTGTAAT AGAAACAGAG AACACCAAAA TCTTTCAATA TAGTGTGCAG 
ATAGAAAAAC CGTTTACAGA AGGGAATCTT TCTGGTTTTA TAAATTATCA AATGATGGAC 
ACTCATGCTG TTCAGCTTGA ATTTTCAGTA AACTTATCAC TATTAGATTT CATTAGGCCA 
TTAAAAATCT CAACTGAAGA CTTCGGCAAA CTCTGGTTAT CCTTTGCTAA TGATGTGAAG 
CAAAATGTAA AAATATCAGA CTCTCAAGCT GCTCTGCCTT CTGCCCTAAA GACCCTGCAA 
CAGAAACTGA GACTTCATGT TGTTGACATC ATAGGCAAAG AAGGGCTGTT GGCCTGTCAG 
CTGCTCCCAT CCATCCCTTG CTTACTGCAT TTCCGAGTTC ATGGTGATGT ATTAGCCCTA 
TGGTTCAGAT CCTCCTGTCC TACTCTTCCT GACTATTTAT TGTATCAGTG TCAAAAGGTG 
ATGGAGGGAT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015451326.1
CDS	65..3439
Translation	MSDIVEKTLTALPGLFLQNQGLGGPAAGKASFSSRLGGLVRGITALTSKHVATVSAPTTTLKMMKECMVRLIYCEMLGYDASFGYIHAIKLAQQGNLLEKRVGYLAVSLFLHESHELLLLLVNTVVKDLQSTNLVEVCMALTVVSQIFPREMIPAVLPLIEDKLQHSKEIIRRKAVLALYKFHLIAPNQVQHIHIKFQKALCDRDVGVMAASLHIYLKMIKENSSGYKDLTGSFVTILKQVVGGKLPVDFNYHSVPAPWLQIQLLRILGLLGKDDQRTSELMYDVLDESLRRAELNHNVTYAILFECVHTVYSIYPKSELLEKAAKCIGKFVLSPKINLKYLGLKALTYVIQQDPTLALQHQMTIIECLDHPDPIIKRETLELLYRITNAQNVTVIVQKMLEYLHQSKEEYIIINLVGKIAELAEKYAPDNAWFIQTMNAVFSVGGDVMHPDIPNNFLRLLAEGFDDETEDQQLRLYAVQSYLTLLDVENVFYPQRFLQVMSWVLGEYSYLLDKETPEEVITKLYKLLMNDTTSSETKAWLIAAVTKLTPQAHSSSIVESLIQEFTVSLDTCMRQHAFELKHLHENVEFMKSLLPVNKSCEDMVVDASLSFLDGYVAEELSQGAAPYKPHHQRQEEKLSQEKVLNFEPYGLSFSSSGFTGRQSPAGISLGSDISGNSAETGLKETNSLKLEGIKKLWGKEGYLPKKESKTGDETEAPPVSQESIMLENVDQTTAKKDQSQVLTQSKEEKEKQLLASSLFVGLGSESTINLLGKADNVSHKFKRKSKVKETKTEETTSAYNTTCPSFSSLSSVTYEDYYLDNLQDRGDKELNKFTLSSELLDSESLTELPLVEKLSNCRLSKPSLFADNNMEVFHPPQFTAASVGNEISLDSCLLEETAEHMHSDPIDVCNDENMSVSSYKIWKDDCLLMVWSVSNKGSSELKSANLEIAPAENFKITEQPGCCLPVIETENTKIFQYSVQIEKPFTEGNLSGFINYQMMDTHAVQLEFSVNLSLLDFIRPLKISTEDFGKLWLSFANDVKQNVKISDSQAALPSALKTLQQKLRLHVVDIIGKEGLLACQLLPSIPCLLHFRVHGDVLALWFRSSCPTLPDYLLYQCQKVMEGS

Target ORF information:

RefSeq Version	XM_015595840.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto adaptor related protein complex 4 epsilon 1 subunit (AP4E1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015595840.1

ORF Insert Sequence:

ATGAGCGACA TAGTGGAGAA GACGCTGACG GCCCTGCCGG GACTCTTCCT GCAGAACCAG 
GGGCTTGGTG GGCCCGCGGC CGGCAAGGCG TCTTTCTCTT CACGACTGGG CGGCCTCGTC 
CGCGGCATCA CCGCGCTCAC CTCCAAGCAC GTAGCAACTG TTTCTGCTCC TACAACAACT 
CTGAAAATGA TGAAGGAATG TATGGTGAGA CTTATATATT GTGAAATGCT TGGATATGAT 
GCTTCCTTTG GCTACATACA TGCAATCAAG TTGGCACAAC AAGGAAACCT TTTAGAAAAA 
AGAGTAGGTT ATTTGGCAGT TTCTTTATTT CTACATGAAA GTCATGAACT GTTGCTTCTC 
CTTGTGAATA CGGTCGTAAA GGACTTGCAG AGCACTAACC TGGTAGAAGT ATGTATGGCA 
CTTACTGTCG TCAGCCAGAT TTTCCCTCGA GAAATGATTC CAGCTGTTCT TCCATTAATA 
GAAGATAAAC TTCAACATTC TAAGGAGATT ATACGAAGAA AAGCTGTTTT AGCATTATAC 
AAATTCCATC TCATTGCTCC TAATCAAGTA CAGCATATTC ATATAAAGTT TCAGAAAGCA 
CTTTGTGACA GAGATGTTGG GGTCATGGCT GCTTCCTTGC ACATATACCT TAAGATGATT 
AAGGAGAATT CATCTGGATA TAAAGACTTG ACTGGGAGTT TTGTAACAAT TTTGAAGCAA 
GTAGTTGGAG GAAAGCTCCC AGTAGATTTC AATTACCACA GTGTGCCAGC ACCATGGTTA 
CAAATTCAGC TCTTGAGAAT ACTAGGACTT CTAGGAAAAG ATGATCAAAG GACAAGTGAA 
TTAATGTATG ATGTTCTTGA TGAATCCTTA CGAAGAGCCG AGTTAAATCA CAATGTCACA 
TATGCTATTT TGTTTGAATG TGTGCATACA GTCTATTCTA TTTATCCTAA ATCAGAATTA 
CTTGAGAAGG CTGCCAAGTG CATTGGGAAA TTTGTTCTAT CACCTAAAAT AAATCTCAAA 
TATTTAGGAC TGAAAGCTCT TACCTATGTT ATCCAACAGG ATCCTACTCT GGCTCTTCAA 
CACCAGATGA CAATAATTGA ATGCTTAGAC CATCCTGATC CCATTATTAA GAGAGAGACT 
CTGGAACTTC TTTACAGAAT TACTAATGCA CAGAATGTAA CAGTGATTGT CCAGAAAATG 
CTTGAATATT TACATCAGAG CAAAGAAGAG TATATTATCA TCAATTTGGT TGGCAAAATT 
GCTGAGCTGG CAGAGAAATA TGCTCCTGAT AATGCGTGGT TTATTCAGAC AATGAATGCT 
GTCTTTTCAG TGGGAGGAGA TGTAATGCAT CCTGATATTC CCAATAACTT CCTGAGACTG 
CTAGCAGAAG GTTTTGATGA TGAAACAGAA GATCAACAAT TAAGACTTTA TGCAGTTCAG 
TCTTACCTCA CGTTACTAGA TGTGGAAAAT GTGTTCTATC CACAGAGATT TCTTCAAGTT 
ATGAGTTGGG TGTTAGGGGA GTATTCCTAC CTCTTAGATA AGGAAACACC AGAAGAAGTT 
ATAACCAAGC TCTACAAGTT ACTTATGAAC GACACCACTT CTTCAGAAAC AAAAGCCTGG 
TTAATTGCTG CTGTGACCAA GTTAACCCCC CAAGCACACT CTTCTAGCAT TGTTGAGAGT 
TTAATCCAGG AATTTACCGT GTCTTTGGAT ACTTGTATGA GACAGCATGC ATTTGAATTA 
AAACATTTGC ATGAGAATGT GGAATTTATG AAGAGCTTGC TTCCAGTTAA TAAGAGTTGT 
GAAGACATGG TGGTAGATGC CTCCTTATCT TTTCTGGATG GTTACGTGGC CGAAGAACTC 
AGTCAGGGGG CAGCACCTTA CAAACCTCAC CACCAAAGGC AGGAAGAAAA ACTTTCTCAG 
GAAAAAGTTC TCAATTTTGA ACCGTATGGA CTCTCCTTTT CTTCATCTGG CTTCACTGGA 
CGACAGTCTC CTGCTGGCAT TTCTCTTGGT TCAGATATAT CTGGGAATAG TGCAGAGACA 
GGGCTGAAAG AGACAAATAG CTTGAAGCTG GAAGGTATAA AGAAATTGTG GGGGAAAGAG 
GGCTATCTTC CCAAGAAGGA AAGCAAAACT GGTGATGAAA CTGAAGCTCC GCCTGTTTCT 
CAAGAGAGTA TAATGTTGGA GAATGTAGAT CAAACCACAG CCAAAAAAGA TCAATCTCAA 
GTCCTCACCC AATCTAAAGA GGAGAAAGAA AAGCAGCTGC TGGCATCATC ATTATTTGTG 
GGGCTAGGAT CAGAAAGTAC AATCAATTTG CTGGGAAAAG CAGATAACGT CTCTCACAAG 
TTCAAAAGAA AATCAAAAGT CAAGGAAACC AAAACTGAAG AAACAACCAG TGCTTATAAT 
ACGACCTGTC CTTCCTTTAG TTCTTTATCA AGTGTGACTT ATGAAGATTA TTATTTGGAT 
AATTTGCAGG ATAGAGGAGA CAAAGAATTA AATAAATTTA CTCTCAGTTC AGAACTTTTG 
GATTCTGAAT CACTCACTGA ACTGCCCTTA GTTGAGAAAC TTTCAAATTG CAGGCTATCT 
AAACCTTCAT TGTTTGCGGA TAACAACATG GAAGTTTTTC ACCCTCCTCA ATTCACTGCA 
GCCTCAGTTG GCAATGAAAT CTCTTTAGAT TCCTGTTTGT TGGAAGAAAC TGCTGAACAC 
ATGCATTCAG ATCCTATAGA TGTCTGTAAT GATGAAAACA TGTCGGTGTC TTCTTACAAA 
ATTTGGAAAG ATGATTGTTT ATTGATGGTC TGGTCAGTTT CTAATAAGGG TAGTTCAGAA 
TTGAAAAGTG CTAACTTAGA AATTGCTCCT GCAGAAAATT TCAAGATCAC CGAGCAACCT 
GGATGCTGCT TGCCTGTAAT AGAAACAGAG AACACCAAAA TCTTTCAATA TAGTGTGCAG 
ATAGAAAAAC CGTTTACAGA AGGGAATCTT TCTGGTTTTA TAAATTATCA AATGATGGAC 
ACTCATGCTG TTCAGCTTGA ATTTTCAGTA AACTTATCAC TATTAGATTT CATTAGGCCA 
TTAAAAATCT CAACTGAAGA CTTCGGCAAA CTCTGGTTAT CCTTTGCTAA TGATGTGAAG 
CAAAATGTAA AAATATCAGA CTCTCAAGCT GCTCTGCCTT CTGCCCTAAA GACCCTGCAA 
CAGAAACTGA GACTTCATGT TGTTGACATC ATAGGCAAAG AAGGGCTGTT GGCCTGTCAG 
CTGCTCCCAT CCATCCCTTG CTTACTGCAT TTCCGAGTTC ATGGTGATGT ATTAGCCCTA 
TGGTTCAGAT CCTCCTGTCC TACTCTTCCT GACTATTTAT TGTATCAGTG TCAAAAGGTG 
ATGGAGGGAT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP4E1 ( XM_025038750.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_024894518.1 Pteropus alecto adaptor related protein complex 4 subunit epsilon 1 (AP4E1), mRNA.

Price & Availability↑

CloneID	OPt81997
Clone ID Related Accession (Same CDS sequence)	XM_025038750.1
Accession Version	XM_025038750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	AP-4 complex subunit epsilon-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006442109.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGAGCGACA TAGTGGAGAA GACGCTGACG GCCCTGCCGG GACTCTTCCT GCAGAACCAG 
GGGCTTGGTG GGCCCGCGGC CGGCAAGGCG TCTTTCTCTT CACGACTGGG CGGCCTCGTC 
CGCGGCATCA CCGCGCTCAC CTCCAAGCAC GTAGGTGCCG GCCCGGCGGG GAGGTCAGGG 
ACGCCCAGGG GACGTCCCCG CGCTTGGAGG CCCTGGTCCG GCGGCGAGCC GACAGGGCGC 
GCCCTCCCGC AGGGCTCAGG GACTGCGATT GTGTCCGTCC GTCTTTGCTT CTGTCTGGAA 
GCGTCACTTT CTTACCCGGA ATCAACTGTT TCTGCTCCTA CAACAACTCT GAAAATGATG 
AAGGAATGTA TGGTGAGACT TATATATTGT GAAATGCTTG GATATGATGC TTCCTTTGGC 
TACATACATG CAATCAAGTT GGCACAACAA GGAAACCTTT TAGAAAAAAG AGTAGGTTAT 
TTGGCAGTTT CTTTATTTCT ACATGAAAGT CATGAACTGT TGCTTCTCCT TGTGAATACG 
GTCGTAAAGG ACTTGCAGAG CACTAACCTG GTAGAAGTAT GTATGGCACT TACTGTCGTC 
AGCCAGATTT TCCCTCGAGA AATGATTCCA GCTGTTCTTC CATTAATAGA AGATAAACTT 
CAACATTCTA AGGAGATTAT ACGAAGAAAA GCTGTTTTAG CATTATACAA ATTCCATCTC 
ATTGCTCCTA ATCAAGTACA GCATATTCAT ATAAAGTTTC AGAAAGCACT TTGTGACAGA 
GATGTTGGGG TCATGGCTGC TTCCTTGCAC ATATACCTTA AGATGATTAA GGAGAATTCA 
TCTGGATATA AAGACTTGAC TGGGAGTTTT GTAACAATTT TGAAGCAAGT AGTTGGAGGA 
AAGCTCCCAG TAGATTTCAA TTACCACAGT GTGCCAGCAC CATGGTTACA AATTCAGCTC 
TTGAGAATAC TAGGACTTCT AGGAAAAGAT GATCAAAGGA CAAGTGAATT AATGTATGAT 
GTTCTTGATG AATCCTTACG AAGAGCCGAG TTAAATCACA ATGTCACATA TGCTATTTTG 
TTTGAATGTG TGCATACAGT CTATTCTATT TATCCTAAAT CAGAATTACT TGAGAAGGCT 
GCCAAGTGCA TTGGGAAATT TGTTCTATCA CCTAAAATAA ATCTCAAATA TTTAGGACTG 
AAAGCTCTTA CCTATGTTAT CCAACAGGAT CCTACTCTGG CTCTTCAACA CCAGATGACA 
ATAATTGAAT GCTTAGACCA TCCTGATCCC ATTATTAAGA GAGAGACTCT GGAACTTCTT 
TACAGAATTA CTAATGCACA GAATGTAACA GTGATTGTCC AGAAAATGCT TGAATATTTA 
CATCAGAGCA AAGAAGAGTA TATTATCATC AATTTGGTTG GCAAAATTGC TGAGCTGGCA 
GAGAAATATG CTCCTGATAA TGCGTGGTTT ATTCAGACAA TGAATGCTGT CTTTTCAGTG 
GGAGGAGATG TAATGCATCC TGATATTCCC AATAACTTCC TGAGACTGCT AGCAGAAGGT 
TTTGATGATG AAACAGAAGA TCAACAATTA AGACTTTATG CAGTTCAGTC TTACCTCACG 
TTACTAGATG TGGAAAATGT GTTCTATCCA CAGAGATTTC TTCAAGTTAT GAGTTGGGTG 
TTAGGGGAGT ATTCCTACCT CTTAGATAAG GAAACACCAG AAGAAGTTAT AACCAAGCTC 
TACAAGTTAC TTATGAACGA CACCACTTCT TCAGAAACAA AAGCCTGGTT AATTGCTGCT 
GTGACCAAGT TAACCCCCCA AGCACACTCT TCTAGCATTG TTGAGAGTTT AATCCAGGAA 
TTTACCGTGT CTTTGGATAC TTGTATGAGA CAGCATGCAT TTGAATTAAA ACATTTGCAT 
GAGAATGTGG AATTTATGAA GAGCTTGCTT CCAGTTAATA AGAGTTGTGA AGACATGGTG 
GTAGATGCCT CCTTATCTTT TCTGGATGGT TACGTGGCCG AAGAACTCAG TCAGGGGGCA 
GCACCTTACA AACCTCACCA CCAAAGGCAG GAAGAAAAAC TTTCTCAGGA AAAAGTTCTC 
AATTTTGAAC CGTATGGACT CTCCTTTTCT TCATCTGGCT TCACTGGACG ACAGTCTCCT 
GCTGGCATTT CTCTTGGTTC AGATATATCT GGGAATAGTG CAGAGACAGG GCTGAAAGAG 
ACAAATAGCT TGAAGCTGGA AGGTATAAAG AAATTGTGGG GGAAAGAGGG CTATCTTCCC 
AAGAAGGAAA GCAAAACTGG TGATGAAACT GAAGCTCCGC CTGTTTCTCA AGAGAGTATA 
ATGTTGGAGA ATGTAGATCA AACCACAGCC AAAAAAGATC AATCTCAAGT CCTCACCCAA 
TCTAAAGAGG AGAAAGAAAA GCAGCTGCTG GCATCATCAT TATTTGTGGG GCTAGGATCA 
GAAAGTACAA TCAATTTGCT GGGAAAAGCA GATAACGTCT CTCACAAGTT CAAAAGAAAA 
TCAAAAGTCA AGGAAACCAA AACTGAAGAA ACAACCAGTG CTTATAATAC GACCTGTCCT 
TCCTTTAGTT CTTTATCAAG TGTGACTTAT GAAGATTATT ATTTGGATAA TTTGCAGGAT 
AGAGGAGACA AAGAATTAAA TAAATTTACT CTCAGTTCAG AACTTTTGGA TTCTGAATCA 
CTCACTGAAC TGCCCTTAGT TGAGAAACTT TCAAATTGCA GGCTATCTAA ACCTTCATTG 
TTTGCGGATA ACAACATGGA AGTTTTTCAC CCTCCTCAAT TCACTGCAGC CTCAGTTGGC 
AATGAAATCT CTTTAGATTC CTGTTTGTTG GAAGAAACTG CTGAACACAT GCATTCAGAT 
CCTATAGATG TCTGTAATGA TGAAAACATG TCGGTGTCTT CTTACAAAAT TTGGAAAGAT 
GATTGTTTAT TGATGGTCTG GTCAGTTTCT AATAAGGGTA GTTCAGAATT GAAAAGTGCT 
AACTTAGAAA TTGCTCCTGC AGAAAATTTC AAGATCACCG AGCAACCTGG ATGCTGCTTG 
CCTGTAATAG AAACAGAGAA CACCAAAATC TTTCAATATA GTGTGCAGAT AGAAAAACCG 
TTTACAGAAG GGAATCTTTC TGGTTTTATA AATTATCAAA TGATGGACAC TCATGCTGTT 
CAGCTTGAAT TTTCAGTAAA CTTATCACTA TTAGATTTCA TTAGGCCATT AAAAATCTCA 
ACTGAAGACT TCGGCAAACT CTGGTTATCC TTTGCTAATG ATGTGAAGCA AAATGTAAAA 
ATATCAGACT CTCAAGCTGC TCTGCCTTCT GCCCTAAAGA CCCTGCAACA GAAACTGAGA 
CTTCATGTTG TTGACATCAT AGGCAAAGAA GGGCTGTTGG CCTGTCAGCT GCTCCCATCC 
ATCCCTTGCT TACTGCATTT CCGAGTTCAT GGTGATGTAT TAGCCCTATG GTTCAGATCC 
TCCTGTCCTA CTCTTCCTGA CTATTTATTG TATCAGTGTC AAAAGGTGAT GGAGGGATCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024894518.1
CDS	105..3647
Translation	MSDIVEKTLTALPGLFLQNQGLGGPAAGKASFSSRLGGLVRGITALTSKHVGAGPAGRSGTPRGRPRAWRPWSGGEPTGRALPQGSGTAIVSVRLCFCLEASLSYPESTVSAPTTTLKMMKECMVRLIYCEMLGYDASFGYIHAIKLAQQGNLLEKRVGYLAVSLFLHESHELLLLLVNTVVKDLQSTNLVEVCMALTVVSQIFPREMIPAVLPLIEDKLQHSKEIIRRKAVLALYKFHLIAPNQVQHIHIKFQKALCDRDVGVMAASLHIYLKMIKENSSGYKDLTGSFVTILKQVVGGKLPVDFNYHSVPAPWLQIQLLRILGLLGKDDQRTSELMYDVLDESLRRAELNHNVTYAILFECVHTVYSIYPKSELLEKAAKCIGKFVLSPKINLKYLGLKALTYVIQQDPTLALQHQMTIIECLDHPDPIIKRETLELLYRITNAQNVTVIVQKMLEYLHQSKEEYIIINLVGKIAELAEKYAPDNAWFIQTMNAVFSVGGDVMHPDIPNNFLRLLAEGFDDETEDQQLRLYAVQSYLTLLDVENVFYPQRFLQVMSWVLGEYSYLLDKETPEEVITKLYKLLMNDTTSSETKAWLIAAVTKLTPQAHSSSIVESLIQEFTVSLDTCMRQHAFELKHLHENVEFMKSLLPVNKSCEDMVVDASLSFLDGYVAEELSQGAAPYKPHHQRQEEKLSQEKVLNFEPYGLSFSSSGFTGRQSPAGISLGSDISGNSAETGLKETNSLKLEGIKKLWGKEGYLPKKESKTGDETEAPPVSQESIMLENVDQTTAKKDQSQVLTQSKEEKEKQLLASSLFVGLGSESTINLLGKADNVSHKFKRKSKVKETKTEETTSAYNTTCPSFSSLSSVTYEDYYLDNLQDRGDKELNKFTLSSELLDSESLTELPLVEKLSNCRLSKPSLFADNNMEVFHPPQFTAASVGNEISLDSCLLEETAEHMHSDPIDVCNDENMSVSSYKIWKDDCLLMVWSVSNKGSSELKSANLEIAPAENFKITEQPGCCLPVIETENTKIFQYSVQIEKPFTEGNLSGFINYQMMDTHAVQLEFSVNLSLLDFIRPLKISTEDFGKLWLSFANDVKQNVKISDSQAALPSALKTLQQKLRLHVVDIIGKEGLLACQLLPSIPCLLHFRVHGDVLALWFRSSCPTLPDYLLYQCQKVMEGS

Target ORF information:

RefSeq Version	XM_025038750.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto adaptor related protein complex 4 subunit epsilon 1 (AP4E1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025038750.1

ORF Insert Sequence:

ATGAGCGACA TAGTGGAGAA GACGCTGACG GCCCTGCCGG GACTCTTCCT GCAGAACCAG 
GGGCTTGGTG GGCCCGCGGC CGGCAAGGCG TCTTTCTCTT CACGACTGGG CGGCCTCGTC 
CGCGGCATCA CCGCGCTCAC CTCCAAGCAC GTAGGTGCCG GCCCGGCGGG GAGGTCAGGG 
ACGCCCAGGG GACGTCCCCG CGCTTGGAGG CCCTGGTCCG GCGGCGAGCC GACAGGGCGC 
GCCCTCCCGC AGGGCTCAGG GACTGCGATT GTGTCCGTCC GTCTTTGCTT CTGTCTGGAA 
GCGTCACTTT CTTACCCGGA ATCAACTGTT TCTGCTCCTA CAACAACTCT GAAAATGATG 
AAGGAATGTA TGGTGAGACT TATATATTGT GAAATGCTTG GATATGATGC TTCCTTTGGC 
TACATACATG CAATCAAGTT GGCACAACAA GGAAACCTTT TAGAAAAAAG AGTAGGTTAT 
TTGGCAGTTT CTTTATTTCT ACATGAAAGT CATGAACTGT TGCTTCTCCT TGTGAATACG 
GTCGTAAAGG ACTTGCAGAG CACTAACCTG GTAGAAGTAT GTATGGCACT TACTGTCGTC 
AGCCAGATTT TCCCTCGAGA AATGATTCCA GCTGTTCTTC CATTAATAGA AGATAAACTT 
CAACATTCTA AGGAGATTAT ACGAAGAAAA GCTGTTTTAG CATTATACAA ATTCCATCTC 
ATTGCTCCTA ATCAAGTACA GCATATTCAT ATAAAGTTTC AGAAAGCACT TTGTGACAGA 
GATGTTGGGG TCATGGCTGC TTCCTTGCAC ATATACCTTA AGATGATTAA GGAGAATTCA 
TCTGGATATA AAGACTTGAC TGGGAGTTTT GTAACAATTT TGAAGCAAGT AGTTGGAGGA 
AAGCTCCCAG TAGATTTCAA TTACCACAGT GTGCCAGCAC CATGGTTACA AATTCAGCTC 
TTGAGAATAC TAGGACTTCT AGGAAAAGAT GATCAAAGGA CAAGTGAATT AATGTATGAT 
GTTCTTGATG AATCCTTACG AAGAGCCGAG TTAAATCACA ATGTCACATA TGCTATTTTG 
TTTGAATGTG TGCATACAGT CTATTCTATT TATCCTAAAT CAGAATTACT TGAGAAGGCT 
GCCAAGTGCA TTGGGAAATT TGTTCTATCA CCTAAAATAA ATCTCAAATA TTTAGGACTG 
AAAGCTCTTA CCTATGTTAT CCAACAGGAT CCTACTCTGG CTCTTCAACA CCAGATGACA 
ATAATTGAAT GCTTAGACCA TCCTGATCCC ATTATTAAGA GAGAGACTCT GGAACTTCTT 
TACAGAATTA CTAATGCACA GAATGTAACA GTGATTGTCC AGAAAATGCT TGAATATTTA 
CATCAGAGCA AAGAAGAGTA TATTATCATC AATTTGGTTG GCAAAATTGC TGAGCTGGCA 
GAGAAATATG CTCCTGATAA TGCGTGGTTT ATTCAGACAA TGAATGCTGT CTTTTCAGTG 
GGAGGAGATG TAATGCATCC TGATATTCCC AATAACTTCC TGAGACTGCT AGCAGAAGGT 
TTTGATGATG AAACAGAAGA TCAACAATTA AGACTTTATG CAGTTCAGTC TTACCTCACG 
TTACTAGATG TGGAAAATGT GTTCTATCCA CAGAGATTTC TTCAAGTTAT GAGTTGGGTG 
TTAGGGGAGT ATTCCTACCT CTTAGATAAG GAAACACCAG AAGAAGTTAT AACCAAGCTC 
TACAAGTTAC TTATGAACGA CACCACTTCT TCAGAAACAA AAGCCTGGTT AATTGCTGCT 
GTGACCAAGT TAACCCCCCA AGCACACTCT TCTAGCATTG TTGAGAGTTT AATCCAGGAA 
TTTACCGTGT CTTTGGATAC TTGTATGAGA CAGCATGCAT TTGAATTAAA ACATTTGCAT 
GAGAATGTGG AATTTATGAA GAGCTTGCTT CCAGTTAATA AGAGTTGTGA AGACATGGTG 
GTAGATGCCT CCTTATCTTT TCTGGATGGT TACGTGGCCG AAGAACTCAG TCAGGGGGCA 
GCACCTTACA AACCTCACCA CCAAAGGCAG GAAGAAAAAC TTTCTCAGGA AAAAGTTCTC 
AATTTTGAAC CGTATGGACT CTCCTTTTCT TCATCTGGCT TCACTGGACG ACAGTCTCCT 
GCTGGCATTT CTCTTGGTTC AGATATATCT GGGAATAGTG CAGAGACAGG GCTGAAAGAG 
ACAAATAGCT TGAAGCTGGA AGGTATAAAG AAATTGTGGG GGAAAGAGGG CTATCTTCCC 
AAGAAGGAAA GCAAAACTGG TGATGAAACT GAAGCTCCGC CTGTTTCTCA AGAGAGTATA 
ATGTTGGAGA ATGTAGATCA AACCACAGCC AAAAAAGATC AATCTCAAGT CCTCACCCAA 
TCTAAAGAGG AGAAAGAAAA GCAGCTGCTG GCATCATCAT TATTTGTGGG GCTAGGATCA 
GAAAGTACAA TCAATTTGCT GGGAAAAGCA GATAACGTCT CTCACAAGTT CAAAAGAAAA 
TCAAAAGTCA AGGAAACCAA AACTGAAGAA ACAACCAGTG CTTATAATAC GACCTGTCCT 
TCCTTTAGTT CTTTATCAAG TGTGACTTAT GAAGATTATT ATTTGGATAA TTTGCAGGAT 
AGAGGAGACA AAGAATTAAA TAAATTTACT CTCAGTTCAG AACTTTTGGA TTCTGAATCA 
CTCACTGAAC TGCCCTTAGT TGAGAAACTT TCAAATTGCA GGCTATCTAA ACCTTCATTG 
TTTGCGGATA ACAACATGGA AGTTTTTCAC CCTCCTCAAT TCACTGCAGC CTCAGTTGGC 
AATGAAATCT CTTTAGATTC CTGTTTGTTG GAAGAAACTG CTGAACACAT GCATTCAGAT 
CCTATAGATG TCTGTAATGA TGAAAACATG TCGGTGTCTT CTTACAAAAT TTGGAAAGAT 
GATTGTTTAT TGATGGTCTG GTCAGTTTCT AATAAGGGTA GTTCAGAATT GAAAAGTGCT 
AACTTAGAAA TTGCTCCTGC AGAAAATTTC AAGATCACCG AGCAACCTGG ATGCTGCTTG 
CCTGTAATAG AAACAGAGAA CACCAAAATC TTTCAATATA GTGTGCAGAT AGAAAAACCG 
TTTACAGAAG GGAATCTTTC TGGTTTTATA AATTATCAAA TGATGGACAC TCATGCTGTT 
CAGCTTGAAT TTTCAGTAAA CTTATCACTA TTAGATTTCA TTAGGCCATT AAAAATCTCA 
ACTGAAGACT TCGGCAAACT CTGGTTATCC TTTGCTAATG ATGTGAAGCA AAATGTAAAA 
ATATCAGACT CTCAAGCTGC TCTGCCTTCT GCCCTAAAGA CCCTGCAACA GAAACTGAGA 
CTTCATGTTG TTGACATCAT AGGCAAAGAA GGGCTGTTGG CCTGTCAGCT GCTCCCATCC 
ATCCCTTGCT TACTGCATTT CCGAGTTCAT GGTGATGTAT TAGCCCTATG GTTCAGATCC 
TCCTGTCCTA CTCTTCCTGA CTATTTATTG TATCAGTGTC AAAAGGTGAT GGAGGGATCC 
TAG