CHAT cDNA ORF clone, Pterocles gutturalis(yellow-throated sandgrouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHAT
Entrez Gene ID	104462706
Full Name	choline O-acetyltransferase
Gene Type	protein-coding
Organism	Pterocles gutturalis(yellow-throated sandgrouse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010074769.1	XP_010073071.1	choline O-acetyltransferase

Pathways from BioSystems
Gene Ontology

The following CHAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPt35025	XM_010074769.1 Latest version!	Pterocles gutturalis choline O-acetyltransferase (CHAT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHAT ( XM_010074769.1 ) cDNA ORF clone, Pterocles gutturalis(yellow-throated sandgrouse) -> XP_010073071.1 Pterocles gutturalis choline O-acetyltransferase (CHAT), mRNA.

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CloneID	OPt35025
Clone ID Related Accession (Same CDS sequence)	XM_010074769.1
Accession Version	XM_010074769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Pterocles gutturalis(yellow-throated sandgrouse)
Product	choline O-acetyltransferase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010114224.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pterocles gutturalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCTAC AGTGCATGAA ACACTTGGTG ACAGAGGAGC AATTTAGAAA GACCAAGGTC 
ATTGTGGAGC AGTTTGGAAT TTCAGGAGGC TTGGGGGAAT CCTTGCAGCA AATCCTTGAG 
GAAAGAAGGG AAAAGACAAC AAACTGGGTG CTTAACTACT GGCTGGATGA CATGTACCTC 
AACAACCGGC TAGCTCTTCC AGTCAATTCA AGCCCAGCAA TTATCTTTGC TCGTCAGTAT 
TTCAACAATG TAAATGACCA GCTGAGGTTT GCTGCCAATC TAATTTCAGG AGTGCAAGAC 
TATAAAGCTT TACTGGACAC ACATGCTTTA CCTGTTGATT TTGCTCGTGG ACAGCTGTCT 
GGTCATCCTC TCTGTATGAA GCAATACTAT GGACTCTTTT CTTCCTATCG TCTTCCAGGA 
CATACCAAAG ATACCCTTGT GGCCCAGAAA AGCAGTGTAA TGCCAGAGCC AGAGCACATC 
ATTGTTGCTT GTAACAATCA GTTTTTTGTT TTGGATGTTG TCATTAATTT CCGTCGTCTC 
AGTGAGGGAG ATCTTTTCAC TCAGTTACGA AAGATAGTCA AAATGGCAGA GAATGAAGAG 
GAAATGCTGC CTCCAGTTGG CCTGCTGACA ACAGACGGAA GAACAGAATG GGCAGAGTCC 
AGGACGATCC TCATGAAAGA CTCTACTAAC CGCGACTCTC TTGACATGAT TGAACGGTGC 
ATATGCCTGG TGTGCCTGGA CAGCTCAAGC GGGGTGGAAC TCAATGATAC AAACATGGCA 
TTGCAACTGC TACATGGGGG AGGCTACCAT AAAAACGGGG CCAATCGTTG GTATGACAAA 
CCTATGCAGT TTGTAGTAGG AAGAGATGGA GTCTGTGGAA CTGTGTGTGA ACATTCCCCT 
TTCGATGGCA TCGTACTGGT GCAGTGCACT GAACACTTGC TCAAGCACAT GAAAGAAAGT 
TCCAAGAAAT TAGTCCGAGC TGATTCAGTG AGTGAGCTTC CTGCTCCACG GAGACTAAGA 
TGGAAATGTT CACCTGAAAT TCAGGCACAT TTGGCATCAT CAGAAGAAAA ACTACAAAGG 
ATAGTAAAAA ATCTGGACTT TATTGCCTAC AAGTTTGAGA ACTATGGAAA GGAATTTATT 
AAGAAACAAA AGATTAGCCC AGATGCCTAC ATTCAAGTAG CACTTCAGCT GGCATTTTAC 
AGATGCCACA GGAGACTTGT ACCTACTTAC GAAAGTGCTT CCATACGCCG ATTTGATGAG 
GGCAGAGTTG ACAATATTAG GTCTGCTACC TTGGAAGCAT TTGCTTTTGT AAAAGCTATG 
ACTGATGACA AGGCAGCTCT ATCGGATTCT GAGAAGATGC AGAGATTTAA AGATGCAATT 
GCAGCTCAGA GTAATTACAC TATTCTGGCT ATTACTGGAA TGGCAATAGA CAATCATCTG 
CTAGGGCTCA GGGAGGTGGC ACGGGAACAC TGCAAGGAGC TGCCAGAGAT ATTTACAGAT 
GAGACATATT TGACAAGCAA TCGATTCATC CTCTCAACCA GCCAGGTTCC AACTACTATG 
GAAATGTTCT GCTGCTATGG GCCTGTGGTG CCTAATGGTT ATGGGGCATG TTACAACCCT 
CAGCCAGAGC ATATATTGTT CTGCATCTCA AGCTTTAAAG ACTGTAAAGA AACCTCCTCA 
GGCATGTTTG CAAAAGCTGT GGAAGAAAGT CTCCTAGAAA TGAGAGCAAT GTGCAACAAA 
AGCAACTCTA CTATGGCAAA GCCACTTGCT AAACAAGAAG CAGCCCCACA GCTACAGGGT 
GACCACAGAC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010073071.1
CDS	56..1870
Translation	MYLQCMKHLVTEEQFRKTKVIVEQFGISGGLGESLQQILEERREKTTNWVLNYWLDDMYLNNRLALPVNSSPAIIFARQYFNNVNDQLRFAANLISGVQDYKALLDTHALPVDFARGQLSGHPLCMKQYYGLFSSYRLPGHTKDTLVAQKSSVMPEPEHIIVACNNQFFVLDVVINFRRLSEGDLFTQLRKIVKMAENEEEMLPPVGLLTTDGRTEWAESRTILMKDSTNRDSLDMIERCICLVCLDSSSGVELNDTNMALQLLHGGGYHKNGANRWYDKPMQFVVGRDGVCGTVCEHSPFDGIVLVQCTEHLLKHMKESSKKLVRADSVSELPAPRRLRWKCSPEIQAHLASSEEKLQRIVKNLDFIAYKFENYGKEFIKKQKISPDAYIQVALQLAFYRCHRRLVPTYESASIRRFDEGRVDNIRSATLEAFAFVKAMTDDKAALSDSEKMQRFKDAIAAQSNYTILAITGMAIDNHLLGLREVAREHCKELPEIFTDETYLTSNRFILSTSQVPTTMEMFCCYGPVVPNGYGACYNPQPEHILFCISSFKDCKETSSGMFAKAVEESLLEMRAMCNKSNSTMAKPLAKQEAAPQLQGDHRL

Target ORF information:

RefSeq Version	XM_010074769.1
Organism	Pterocles gutturalis(yellow-throated sandgrouse)
Definition	Pterocles gutturalis choline O-acetyltransferase (CHAT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010074769.1

ORF Insert Sequence:

ATGTACCTAC AGTGCATGAA ACACTTGGTG ACAGAGGAGC AATTTAGAAA GACCAAGGTC 
ATTGTGGAGC AGTTTGGAAT TTCAGGAGGC TTGGGGGAAT CCTTGCAGCA AATCCTTGAG 
GAAAGAAGGG AAAAGACAAC AAACTGGGTG CTTAACTACT GGCTGGATGA CATGTACCTC 
AACAACCGGC TAGCTCTTCC AGTCAATTCA AGCCCAGCAA TTATCTTTGC TCGTCAGTAT 
TTCAACAATG TAAATGACCA GCTGAGGTTT GCTGCCAATC TAATTTCAGG AGTGCAAGAC 
TATAAAGCTT TACTGGACAC ACATGCTTTA CCTGTTGATT TTGCTCGTGG ACAGCTGTCT 
GGTCATCCTC TCTGTATGAA GCAATACTAT GGACTCTTTT CTTCCTATCG TCTTCCAGGA 
CATACCAAAG ATACCCTTGT GGCCCAGAAA AGCAGTGTAA TGCCAGAGCC AGAGCACATC 
ATTGTTGCTT GTAACAATCA GTTTTTTGTT TTGGATGTTG TCATTAATTT CCGTCGTCTC 
AGTGAGGGAG ATCTTTTCAC TCAGTTACGA AAGATAGTCA AAATGGCAGA GAATGAAGAG 
GAAATGCTGC CTCCAGTTGG CCTGCTGACA ACAGACGGAA GAACAGAATG GGCAGAGTCC 
AGGACGATCC TCATGAAAGA CTCTACTAAC CGCGACTCTC TTGACATGAT TGAACGGTGC 
ATATGCCTGG TGTGCCTGGA CAGCTCAAGC GGGGTGGAAC TCAATGATAC AAACATGGCA 
TTGCAACTGC TACATGGGGG AGGCTACCAT AAAAACGGGG CCAATCGTTG GTATGACAAA 
CCTATGCAGT TTGTAGTAGG AAGAGATGGA GTCTGTGGAA CTGTGTGTGA ACATTCCCCT 
TTCGATGGCA TCGTACTGGT GCAGTGCACT GAACACTTGC TCAAGCACAT GAAAGAAAGT 
TCCAAGAAAT TAGTCCGAGC TGATTCAGTG AGTGAGCTTC CTGCTCCACG GAGACTAAGA 
TGGAAATGTT CACCTGAAAT TCAGGCACAT TTGGCATCAT CAGAAGAAAA ACTACAAAGG 
ATAGTAAAAA ATCTGGACTT TATTGCCTAC AAGTTTGAGA ACTATGGAAA GGAATTTATT 
AAGAAACAAA AGATTAGCCC AGATGCCTAC ATTCAAGTAG CACTTCAGCT GGCATTTTAC 
AGATGCCACA GGAGACTTGT ACCTACTTAC GAAAGTGCTT CCATACGCCG ATTTGATGAG 
GGCAGAGTTG ACAATATTAG GTCTGCTACC TTGGAAGCAT TTGCTTTTGT AAAAGCTATG 
ACTGATGACA AGGCAGCTCT ATCGGATTCT GAGAAGATGC AGAGATTTAA AGATGCAATT 
GCAGCTCAGA GTAATTACAC TATTCTGGCT ATTACTGGAA TGGCAATAGA CAATCATCTG 
CTAGGGCTCA GGGAGGTGGC ACGGGAACAC TGCAAGGAGC TGCCAGAGAT ATTTACAGAT 
GAGACATATT TGACAAGCAA TCGATTCATC CTCTCAACCA GCCAGGTTCC AACTACTATG 
GAAATGTTCT GCTGCTATGG GCCTGTGGTG CCTAATGGTT ATGGGGCATG TTACAACCCT 
CAGCCAGAGC ATATATTGTT CTGCATCTCA AGCTTTAAAG ACTGTAAAGA AACCTCCTCA 
GGCATGTTTG CAAAAGCTGT GGAAGAAAGT CTCCTAGAAA TGAGAGCAAT GTGCAACAAA 
AGCAACTCTA CTATGGCAAA GCCACTTGCT AAACAAGAAG CAGCCCCACA GCTACAGGGT 
GACCACAGAC TCTAA