AARS cDNA ORF clone, Pseudopodoces humilis(Tibetan ground-tit)

Gene
Clones
gRNAs

Summary

Gene Symbol	AARS
Entrez Gene ID	102110588
Full Name	alanyl-tRNA synthetase
Gene Type	protein-coding
Organism	Pseudopodoces humilis(Tibetan ground-tit)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005526283.2	XP_005526340.1	alanine--tRNA ligase, cytoplasmic isoform X1
XM_014255519.1	XP_014110994.1	alanine--tRNA ligase, cytoplasmic isoform X2

Pathways from BioSystems
Gene Ontology

The following AARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPs18094	XM_005526283.2 Latest version!	Pseudopodoces humilis alanyl-tRNA synthetase (AARS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPs18095	XM_014255519.1 Latest version!	Pseudopodoces humilis alanyl-tRNA synthetase (AARS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AARS ( XM_005526283.2 ) cDNA ORF clone, Pseudopodoces humilis(Tibetan ground-tit) -> XP_005526340.1 Pseudopodoces humilis alanyl-tRNA synthetase (AARS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPs18094
Clone ID Related Accession (Same CDS sequence)	XM_005526283.2
Accession Version	XM_005526283.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-20
Organism	Pseudopodoces humilis(Tibetan ground-tit)
Product	alanine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005087583.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 21, 2015 this sequence version replaced XM_005526283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pseudopodoces humilis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACCC CGCTCACTGC CAGCCAGATC CGGCAACGGT TCATTGACTT CTTCAAGGAA 
AACAAGCACA CCTATGTGCA CTCATCTTCT ACAATCCCCC TGGATGACCC CACACTGCTC 
TTTGCCAATG CTGGTATGAA TCAGTTCAAA CCCATCTTCC TCAATACCAT CGACCCCTCA 
CACCCGCTTG CTAAGCTGAG CAGAGCCACC AACACTCAGA AATGCATCCG AGCAGGGGGC 
AAGCACAATG ACCTGGATGA TGTGGGGAAA GATGTGTACC ACCACACCTT CTTTGAGATG 
TTGGGGTCCT GGTCCTTCGG GGATTATTTC AAGGAACTTG CTTGTAAACT GGCACTGGAA 
CTTCTCACCA AGGAGTTTGG CATCCCTGCT GAGAGACTCT ATGTCACTTA CTTTGGTGGA 
AATGAAGCTG CAGGACTGCA ACCAGACCTA GAGTGCAAGC AGATTTGGTT GGATTTGGGG 
CTGGCTGAGG GCAGGATTCT GCCTGGCAGT ATGAAGGATA ATTTCTGGGA AATGGGAGAC 
ACTGGGCCCT GTGGCCCTTG CAGCGAGATC CACTATGACC GGATTGGGGA CAGAGATGCT 
TCACACCTGG TCAATCAGGA TGACCCCAAT GTCCTGGAGA TCTGGAACCT GGTGTTCATA 
CAGTTCAATA GGGAAGCTGA TGGGACACTG AAACCCCTTC CTAAGAAAAG TATTGATACT 
GGGATGGGCC TGGAGAGGCT GGTCTCTGTG CTGCAGAACA AGATGTCCAA CTATGACACT 
GACCTTTTCC TTCCTTACTT TGAAGCCATC CAAAAGGGCA CAGGTGCCAG GCCGTACCTG 
GGGCAGGTTG GGGCTGAGGA TGCTGATGGA ATAGACATGG CCTACCGTGT GCTGGCTGAC 
CATGCACGGA CCATCACCCT GGCCCTCTCG GACGGGGGCA GACCTGACAA CACTGGCAGA 
GGGTATGTGT TGAGGCGGAT TCTCCGACGG GCTGTGCGCT ACTCCCATGA GAAGCTCAAT 
GCCCCCAAGG GTTTCTTTGC TACTCTGGTT GATGTGGTGG TGCAGTCACT GGGAGATGCC 
TTTCCTGAGT TGAAAAAGGA TCCAGATATG GTAAAGGACA TTATCAACGA AGAAGAGGAT 
CAGTTCCTGA AAACACTGAG CAGAGGCCGT CGCATCCTGG ACAGGAAGAT ACAGAGCCTT 
GGGGACAGTA AAGTCATTCC TGGTGACACT GCCTGGCTGC TGTATGACAC CTACGGTTTC 
CCTGCGGATC TCACTGGGCT GATTGCAGAG GAGAAGGGCC TTGTTGTAGA CATGGAGGGC 
TTTGAAGAAG AGCGGAAAAA TGCTCAGCTC AAATCCCAGG GCAAGGGTGC TGGAGGGGAG 
GATCTCCTCA TGCTGGACAT CTATGCCATT GAAGAGCTAA GAGCCCAGGG GCTGGAGGTT 
ACAGATGACT CACCAAAATA CAACTATGCT TCAGATCCAA GCGGTACCTA TGATTTCGGG 
AGCCTCGTGG CCACAGTGAA AGCCATCCGC AGGGAGAAGA GGTTTGTGGA GGAGGCATCC 
ACTGGCCAGG AATGCGGGAT AGTGCTGGAC CGGACCTGCT TCTACGCCGA GCAGGGTGGG 
CAGATCTATG ACCAGGGCTA CATGGTCAAG GATGACGACA GCAGGGAGGA TAAAACAGAA 
TTCACTGTGA AGAACGTGCA GGTCCGAGGA GGCTACGTGC TCCACATAGG AACTCTGTAT 
GGAAGCTTGA AAGTAGGAGA TCAGGTCCAC CTGACTATTG ATGAGGCCAG GCGGAGGCCG 
GTCATGAGCA ACCACACGGC CACCCACATC CTCAACTTTG CCCTGCGCTC TGTGCTGGGG 
GAGGCTGACC AGAGGGGGTC CCTGGTGGCA CCTGACAGGC TGCGCTTCGA CTTCACAGCC 
AAGGGAGCCC TGTCCACTCA GGAAATCAAG AAAGTTGAAG GGATTGCCAA CCAGATGATT 
GAGGAGTCAA AGACAGTGTA TGCCAAGGAC TGCCCTCTTG CAGCAGCCAA AGCTATCCAA 
GGACTTCGGG CAGTTTTTGA TGAGACCTAC CCCGATCCTG TCCGTGTGGT CTCCATTGGC 
ATCCCTGTGG AGGAGCTGCT GGCTGACCCC TCTGGCCCTG GGGGCTCCAT CACCTCTATT 
GAGTTCTGCG GAGGGACGCA CTTGCAGAAC TCCAGCCATG CTGGCCCCTT TGTGATTGTT 
TCAGAAGAGG CAATTGCTAA AGGCATTCGG AGGATAGTTG CAGTCACTGG GGCTGAAGCT 
CGGAAGGCCC TCAGGAAAGT TGACAGCCTC AGGAAGCTGC TGTCAGCCCT GGAGGCCAAG 
GTGAAGGTCC AGACAGCTCC CAACAAGGAT GTGCAGAAGG AGATCACGGA TCTCGGTGAG 
ACACTGGCCA CTGCTGTTAT CCCACAGTGG CAGAAAGATG AACTGAGAGA GGCTGTTAAA 
GCACTGAAGA AAGTCATGGA TGACCTGGAC CGAGCAAGCA AAGCCGATAT CCAGAAAAGA 
GTACTGGAAA AGACCAAGCA AGTTATTGAG AGTCACCCTA ACCAGCCATG GGTCATCATG 
GAGATGGAAA ATGGAGCTTC AGCAAAGGCC CTGAATGAAT CTCTCAAGCT CTTCAAGACT 
AATTCCCCCC AAACTGCTGC TATGCTTTTT GCTGTGGATA ATGAAGCTGG CAGGATCACC 
TGCCTGTGTC AAGTACCCCA GGAGACTGCA AATAAGGGCC TGAAGGCCAG CCAGTGGGTG 
CAGGAAGTGT CTGGCTTGAT GGACGGCAAA GGCGGTGGAA AGGACATCTC TGCACAGGCA 
ACAGGCAAGA ATGTGGGCTG CCTGCAGGAA GCCCTGAAAC TGGCCACGGA CTTTGCCAGG 
CTCCGCCTGG GGGAGCTGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005526340.1
CDS	103..3009
Translation	METPLTASQIRQRFIDFFKENKHTYVHSSSTIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPLAKLSRATNTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKLALELLTKEFGIPAERLYVTYFGGNEAAGLQPDLECKQIWLDLGLAEGRILPGSMKDNFWEMGDTGPCGPCSEIHYDRIGDRDASHLVNQDDPNVLEIWNLVFIQFNREADGTLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFLPYFEAIQKGTGARPYLGQVGAEDADGIDMAYRVLADHARTITLALSDGGRPDNTGRGYVLRRILRRAVRYSHEKLNAPKGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEDQFLKTLSRGRRILDRKIQSLGDSKVIPGDTAWLLYDTYGFPADLTGLIAEEKGLVVDMEGFEEERKNAQLKSQGKGAGGEDLLMLDIYAIEELRAQGLEVTDDSPKYNYASDPSGTYDFGSLVATVKAIRREKRFVEEASTGQECGIVLDRTCFYAEQGGQIYDQGYMVKDDDSREDKTEFTVKNVQVRGGYVLHIGTLYGSLKVGDQVHLTIDEARRRPVMSNHTATHILNFALRSVLGEADQRGSLVAPDRLRFDFTAKGALSTQEIKKVEGIANQMIEESKTVYAKDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGIPVEELLADPSGPGGSITSIEFCGGTHLQNSSHAGPFVIVSEEAIAKGIRRIVAVTGAEARKALRKVDSLRKLLSALEAKVKVQTAPNKDVQKEITDLGETLATAVIPQWQKDELREAVKALKKVMDDLDRASKADIQKRVLEKTKQVIESHPNQPWVIMEMENGASAKALNESLKLFKTNSPQTAAMLFAVDNEAGRITCLCQVPQETANKGLKASQWVQEVSGLMDGKGGGKDISAQATGKNVGCLQEALKLATDFARLRLGELKN

Target ORF information:

RefSeq Version	XM_005526283.2
Organism	Pseudopodoces humilis(Tibetan ground-tit)
Definition	Pseudopodoces humilis alanyl-tRNA synthetase (AARS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005526283.2

ORF Insert Sequence:

ATGGAGACCC CGCTCACTGC CAGCCAGATC CGGCAACGGT TCATTGACTT CTTCAAGGAA 
AACAAGCACA CCTATGTGCA CTCATCTTCT ACAATCCCCC TGGATGACCC CACACTGCTC 
TTTGCCAATG CTGGTATGAA TCAGTTCAAA CCCATCTTCC TCAATACCAT CGACCCCTCA 
CACCCGCTTG CTAAGCTGAG CAGAGCCACC AACACTCAGA AATGCATCCG AGCAGGGGGC 
AAGCACAATG ACCTGGATGA TGTGGGGAAA GATGTGTACC ACCACACCTT CTTTGAGATG 
TTGGGGTCCT GGTCCTTCGG GGATTATTTC AAGGAACTTG CTTGTAAACT GGCACTGGAA 
CTTCTCACCA AGGAGTTTGG CATCCCTGCT GAGAGACTCT ATGTCACTTA CTTTGGTGGA 
AATGAAGCTG CAGGACTGCA ACCAGACCTA GAGTGCAAGC AGATTTGGTT GGATTTGGGG 
CTGGCTGAGG GCAGGATTCT GCCTGGCAGT ATGAAGGATA ATTTCTGGGA AATGGGAGAC 
ACTGGGCCCT GTGGCCCTTG CAGCGAGATC CACTATGACC GGATTGGGGA CAGAGATGCT 
TCACACCTGG TCAATCAGGA TGACCCCAAT GTCCTGGAGA TCTGGAACCT GGTGTTCATA 
CAGTTCAATA GGGAAGCTGA TGGGACACTG AAACCCCTTC CTAAGAAAAG TATTGATACT 
GGGATGGGCC TGGAGAGGCT GGTCTCTGTG CTGCAGAACA AGATGTCCAA CTATGACACT 
GACCTTTTCC TTCCTTACTT TGAAGCCATC CAAAAGGGCA CAGGTGCCAG GCCGTACCTG 
GGGCAGGTTG GGGCTGAGGA TGCTGATGGA ATAGACATGG CCTACCGTGT GCTGGCTGAC 
CATGCACGGA CCATCACCCT GGCCCTCTCG GACGGGGGCA GACCTGACAA CACTGGCAGA 
GGGTATGTGT TGAGGCGGAT TCTCCGACGG GCTGTGCGCT ACTCCCATGA GAAGCTCAAT 
GCCCCCAAGG GTTTCTTTGC TACTCTGGTT GATGTGGTGG TGCAGTCACT GGGAGATGCC 
TTTCCTGAGT TGAAAAAGGA TCCAGATATG GTAAAGGACA TTATCAACGA AGAAGAGGAT 
CAGTTCCTGA AAACACTGAG CAGAGGCCGT CGCATCCTGG ACAGGAAGAT ACAGAGCCTT 
GGGGACAGTA AAGTCATTCC TGGTGACACT GCCTGGCTGC TGTATGACAC CTACGGTTTC 
CCTGCGGATC TCACTGGGCT GATTGCAGAG GAGAAGGGCC TTGTTGTAGA CATGGAGGGC 
TTTGAAGAAG AGCGGAAAAA TGCTCAGCTC AAATCCCAGG GCAAGGGTGC TGGAGGGGAG 
GATCTCCTCA TGCTGGACAT CTATGCCATT GAAGAGCTAA GAGCCCAGGG GCTGGAGGTT 
ACAGATGACT CACCAAAATA CAACTATGCT TCAGATCCAA GCGGTACCTA TGATTTCGGG 
AGCCTCGTGG CCACAGTGAA AGCCATCCGC AGGGAGAAGA GGTTTGTGGA GGAGGCATCC 
ACTGGCCAGG AATGCGGGAT AGTGCTGGAC CGGACCTGCT TCTACGCCGA GCAGGGTGGG 
CAGATCTATG ACCAGGGCTA CATGGTCAAG GATGACGACA GCAGGGAGGA TAAAACAGAA 
TTCACTGTGA AGAACGTGCA GGTCCGAGGA GGCTACGTGC TCCACATAGG AACTCTGTAT 
GGAAGCTTGA AAGTAGGAGA TCAGGTCCAC CTGACTATTG ATGAGGCCAG GCGGAGGCCG 
GTCATGAGCA ACCACACGGC CACCCACATC CTCAACTTTG CCCTGCGCTC TGTGCTGGGG 
GAGGCTGACC AGAGGGGGTC CCTGGTGGCA CCTGACAGGC TGCGCTTCGA CTTCACAGCC 
AAGGGAGCCC TGTCCACTCA GGAAATCAAG AAAGTTGAAG GGATTGCCAA CCAGATGATT 
GAGGAGTCAA AGACAGTGTA TGCCAAGGAC TGCCCTCTTG CAGCAGCCAA AGCTATCCAA 
GGACTTCGGG CAGTTTTTGA TGAGACCTAC CCCGATCCTG TCCGTGTGGT CTCCATTGGC 
ATCCCTGTGG AGGAGCTGCT GGCTGACCCC TCTGGCCCTG GGGGCTCCAT CACCTCTATT 
GAGTTCTGCG GAGGGACGCA CTTGCAGAAC TCCAGCCATG CTGGCCCCTT TGTGATTGTT 
TCAGAAGAGG CAATTGCTAA AGGCATTCGG AGGATAGTTG CAGTCACTGG GGCTGAAGCT 
CGGAAGGCCC TCAGGAAAGT TGACAGCCTC AGGAAGCTGC TGTCAGCCCT GGAGGCCAAG 
GTGAAGGTCC AGACAGCTCC CAACAAGGAT GTGCAGAAGG AGATCACGGA TCTCGGTGAG 
ACACTGGCCA CTGCTGTTAT CCCACAGTGG CAGAAAGATG AACTGAGAGA GGCTGTTAAA 
GCACTGAAGA AAGTCATGGA TGACCTGGAC CGAGCAAGCA AAGCCGATAT CCAGAAAAGA 
GTACTGGAAA AGACCAAGCA AGTTATTGAG AGTCACCCTA ACCAGCCATG GGTCATCATG 
GAGATGGAAA ATGGAGCTTC AGCAAAGGCC CTGAATGAAT CTCTCAAGCT CTTCAAGACT 
AATTCCCCCC AAACTGCTGC TATGCTTTTT GCTGTGGATA ATGAAGCTGG CAGGATCACC 
TGCCTGTGTC AAGTACCCCA GGAGACTGCA AATAAGGGCC TGAAGGCCAG CCAGTGGGTG 
CAGGAAGTGT CTGGCTTGAT GGACGGCAAA GGCGGTGGAA AGGACATCTC TGCACAGGCA 
ACAGGCAAGA ATGTGGGCTG CCTGCAGGAA GCCCTGAAAC TGGCCACGGA CTTTGCCAGG 
CTCCGCCTGG GGGAGCTGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AARS ( XM_014255519.1 ) cDNA ORF clone, Pseudopodoces humilis(Tibetan ground-tit) -> XP_014110994.1 Pseudopodoces humilis alanyl-tRNA synthetase (AARS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPs18095
Clone ID Related Accession (Same CDS sequence)	XM_014255519.1
Accession Version	XM_014255519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-20
Organism	Pseudopodoces humilis(Tibetan ground-tit)
Product	alanine--tRNA ligase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005087583.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pseudopodoces humilis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACTC ATCTTCTACA ATCCCCCTGG ATGACCCCAC ACTGCTCTTT GCCAATGCTG 
TTCAAACCCA TCTTCCTCAA TACCATCGAC CCCTCACACC CGCTTGCTAA GCTGAGCAGA 
GCCACCAACA CTCAGAAATG CATCCGAGCA GGGGGCAAGC ACAATGACCT GGATGATGTG 
GGGAAAGATG TGTACCACCA CACCTTCTTT GAGATGTTGG GGTCCTGGTC CTTCGGGGAT 
TATTTCAAGG AACTTGCTTG TAAACTGGCA CTGGAACTTC TCACCAAGGA GTTTGGCATC 
CCTGCTGAGA GACTCTATGT CACTTACTTT GGTGGAAATG AAGCTGCAGG ACTGCAACCA 
GACCTAGAGT GCAAGCAGAT TTGGTTGGAT TTGGGGCTGG CTGAGGGCAG GATTCTGCCT 
GGCAGTATGA AGGATAATTT CTGGGAAATG GGAGACACTG GGCCCTGTGG CCCTTGCAGC 
GAGATCCACT ATGACCGGAT TGGGGACAGA GATGCTTCAC ACCTGGTCAA TCAGGATGAC 
CCCAATGTCC TGGAGATCTG GAACCTGGTG TTCATACAGT TCAATAGGGA AGCTGATGGG 
ACACTGAAAC CCCTTCCTAA GAAAAGTATT GATACTGGGA TGGGCCTGGA GAGGCTGGTC 
TCTGTGCTGC AGAACAAGAT GTCCAACTAT GACACTGACC TTTTCCTTCC TTACTTTGAA 
GCCATCCAAA AGGGCACAGG TGCCAGGCCG TACCTGGGGC AGGTTGGGGC TGAGGATGCT 
GATGGAATAG ACATGGCCTA CCGTGTGCTG GCTGACCATG CACGGACCAT CACCCTGGCC 
CTCTCGGACG GGGGCAGACC TGACAACACT GGCAGAGGGT ATGTGTTGAG GCGGATTCTC 
CGACGGGCTG TGCGCTACTC CCATGAGAAG CTCAATGCCC CCAAGGGTTT CTTTGCTACT 
CTGGTTGATG TGGTGGTGCA GTCACTGGGA GATGCCTTTC CTGAGTTGAA AAAGGATCCA 
GATATGGTAA AGGACATTAT CAACGAAGAA GAGGATCAGT TCCTGAAAAC ACTGAGCAGA 
GGCCGTCGCA TCCTGGACAG GAAGATACAG AGCCTTGGGG ACAGTAAAGT CATTCCTGGT 
GACACTGCCT GGCTGCTGTA TGACACCTAC GGTTTCCCTG CGGATCTCAC TGGGCTGATT 
GCAGAGGAGA AGGGCCTTGT TGTAGACATG GAGGGCTTTG AAGAAGAGCG GAAAAATGCT 
CAGCTCAAAT CCCAGGGCAA GGGTGCTGGA GGGGAGGATC TCCTCATGCT GGACATCTAT 
GCCATTGAAG AGCTAAGAGC CCAGGGGCTG GAGGTTACAG ATGACTCACC AAAATACAAC 
TATGCTTCAG ATCCAAGCGG TACCTATGAT TTCGGGAGCC TCGTGGCCAC AGTGAAAGCC 
ATCCGCAGGG AGAAGAGGTT TGTGGAGGAG GCATCCACTG GCCAGGAATG CGGGATAGTG 
CTGGACCGGA CCTGCTTCTA CGCCGAGCAG GGTGGGCAGA TCTATGACCA GGGCTACATG 
GTCAAGGATG ACGACAGCAG GGAGGATAAA ACAGAATTCA CTGTGAAGAA CGTGCAGGTC 
CGAGGAGGCT ACGTGCTCCA CATAGGAACT CTGTATGGAA GCTTGAAAGT AGGAGATCAG 
GTCCACCTGA CTATTGATGA GGCCAGGCGG AGGCCGGTCA TGAGCAACCA CACGGCCACC 
CACATCCTCA ACTTTGCCCT GCGCTCTGTG CTGGGGGAGG CTGACCAGAG GGGGTCCCTG 
GTGGCACCTG ACAGGCTGCG CTTCGACTTC ACAGCCAAGG GAGCCCTGTC CACTCAGGAA 
ATCAAGAAAG TTGAAGGGAT TGCCAACCAG ATGATTGAGG AGTCAAAGAC AGTGTATGCC 
AAGGACTGCC CTCTTGCAGC AGCCAAAGCT ATCCAAGGAC TTCGGGCAGT TTTTGATGAG 
ACCTACCCCG ATCCTGTCCG TGTGGTCTCC ATTGGCATCC CTGTGGAGGA GCTGCTGGCT 
GACCCCTCTG GCCCTGGGGG CTCCATCACC TCTATTGAGT TCTGCGGAGG GACGCACTTG 
CAGAACTCCA GCCATGCTGG CCCCTTTGTG ATTGTTTCAG AAGAGGCAAT TGCTAAAGGC 
ATTCGGAGGA TAGTTGCAGT CACTGGGGCT GAAGCTCGGA AGGCCCTCAG GAAAGTTGAC 
AGCCTCAGGA AGCTGCTGTC AGCCCTGGAG GCCAAGGTGA AGGTCCAGAC AGCTCCCAAC 
AAGGATGTGC AGAAGGAGAT CACGGATCTC GGTGAGACAC TGGCCACTGC TGTTATCCCA 
CAGTGGCAGA AAGATGAACT GAGAGAGGCT GTTAAAGCAC TGAAGAAAGT CATGGATGAC 
CTGGACCGAG CAAGCAAAGC CGATATCCAG AAAAGAGTAC TGGAAAAGAC CAAGCAAGTT 
ATTGAGAGTC ACCCTAACCA GCCATGGGTC ATCATGGAGA TGGAAAATGG AGCTTCAGCA 
AAGGCCCTGA ATGAATCTCT CAAGCTCTTC AAGACTAATT CCCCCCAAAC TGCTGCTATG 
CTTTTTGCTG TGGATAATGA AGCTGGCAGG ATCACCTGCC TGTGTCAAGT ACCCCAGGAG 
ACTGCAAATA AGGGCCTGAA GGCCAGCCAG TGGGTGCAGG AAGTGTCTGG CTTGATGGAC 
GGCAAAGGCG GTGGAAAGGA CATCTCTGCA CAGGCAACAG GCAAGAATGT GGGCTGCCTG 
CAGGAAGCCC TGAAACTGGC CACGGACTTT GCCAGGCTCC GCCTGGGGGA GCTGAAGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014110994.1
CDS	24..2846
Translation	MCTHLLQSPWMTPHCSLPMLFKPIFLNTIDPSHPLAKLSRATNTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKLALELLTKEFGIPAERLYVTYFGGNEAAGLQPDLECKQIWLDLGLAEGRILPGSMKDNFWEMGDTGPCGPCSEIHYDRIGDRDASHLVNQDDPNVLEIWNLVFIQFNREADGTLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFLPYFEAIQKGTGARPYLGQVGAEDADGIDMAYRVLADHARTITLALSDGGRPDNTGRGYVLRRILRRAVRYSHEKLNAPKGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEDQFLKTLSRGRRILDRKIQSLGDSKVIPGDTAWLLYDTYGFPADLTGLIAEEKGLVVDMEGFEEERKNAQLKSQGKGAGGEDLLMLDIYAIEELRAQGLEVTDDSPKYNYASDPSGTYDFGSLVATVKAIRREKRFVEEASTGQECGIVLDRTCFYAEQGGQIYDQGYMVKDDDSREDKTEFTVKNVQVRGGYVLHIGTLYGSLKVGDQVHLTIDEARRRPVMSNHTATHILNFALRSVLGEADQRGSLVAPDRLRFDFTAKGALSTQEIKKVEGIANQMIEESKTVYAKDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGIPVEELLADPSGPGGSITSIEFCGGTHLQNSSHAGPFVIVSEEAIAKGIRRIVAVTGAEARKALRKVDSLRKLLSALEAKVKVQTAPNKDVQKEITDLGETLATAVIPQWQKDELREAVKALKKVMDDLDRASKADIQKRVLEKTKQVIESHPNQPWVIMEMENGASAKALNESLKLFKTNSPQTAAMLFAVDNEAGRITCLCQVPQETANKGLKASQWVQEVSGLMDGKGGGKDISAQATGKNVGCLQEALKLATDFARLRLGELKN

Target ORF information:

RefSeq Version	XM_014255519.1
Organism	Pseudopodoces humilis(Tibetan ground-tit)
Definition	Pseudopodoces humilis alanyl-tRNA synthetase (AARS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014255519.1

ORF Insert Sequence:

ATGTGCACTC ATCTTCTACA ATCCCCCTGG ATGACCCCAC ACTGCTCTTT GCCAATGCTG 
TTCAAACCCA TCTTCCTCAA TACCATCGAC CCCTCACACC CGCTTGCTAA GCTGAGCAGA 
GCCACCAACA CTCAGAAATG CATCCGAGCA GGGGGCAAGC ACAATGACCT GGATGATGTG 
GGGAAAGATG TGTACCACCA CACCTTCTTT GAGATGTTGG GGTCCTGGTC CTTCGGGGAT 
TATTTCAAGG AACTTGCTTG TAAACTGGCA CTGGAACTTC TCACCAAGGA GTTTGGCATC 
CCTGCTGAGA GACTCTATGT CACTTACTTT GGTGGAAATG AAGCTGCAGG ACTGCAACCA 
GACCTAGAGT GCAAGCAGAT TTGGTTGGAT TTGGGGCTGG CTGAGGGCAG GATTCTGCCT 
GGCAGTATGA AGGATAATTT CTGGGAAATG GGAGACACTG GGCCCTGTGG CCCTTGCAGC 
GAGATCCACT ATGACCGGAT TGGGGACAGA GATGCTTCAC ACCTGGTCAA TCAGGATGAC 
CCCAATGTCC TGGAGATCTG GAACCTGGTG TTCATACAGT TCAATAGGGA AGCTGATGGG 
ACACTGAAAC CCCTTCCTAA GAAAAGTATT GATACTGGGA TGGGCCTGGA GAGGCTGGTC 
TCTGTGCTGC AGAACAAGAT GTCCAACTAT GACACTGACC TTTTCCTTCC TTACTTTGAA 
GCCATCCAAA AGGGCACAGG TGCCAGGCCG TACCTGGGGC AGGTTGGGGC TGAGGATGCT 
GATGGAATAG ACATGGCCTA CCGTGTGCTG GCTGACCATG CACGGACCAT CACCCTGGCC 
CTCTCGGACG GGGGCAGACC TGACAACACT GGCAGAGGGT ATGTGTTGAG GCGGATTCTC 
CGACGGGCTG TGCGCTACTC CCATGAGAAG CTCAATGCCC CCAAGGGTTT CTTTGCTACT 
CTGGTTGATG TGGTGGTGCA GTCACTGGGA GATGCCTTTC CTGAGTTGAA AAAGGATCCA 
GATATGGTAA AGGACATTAT CAACGAAGAA GAGGATCAGT TCCTGAAAAC ACTGAGCAGA 
GGCCGTCGCA TCCTGGACAG GAAGATACAG AGCCTTGGGG ACAGTAAAGT CATTCCTGGT 
GACACTGCCT GGCTGCTGTA TGACACCTAC GGTTTCCCTG CGGATCTCAC TGGGCTGATT 
GCAGAGGAGA AGGGCCTTGT TGTAGACATG GAGGGCTTTG AAGAAGAGCG GAAAAATGCT 
CAGCTCAAAT CCCAGGGCAA GGGTGCTGGA GGGGAGGATC TCCTCATGCT GGACATCTAT 
GCCATTGAAG AGCTAAGAGC CCAGGGGCTG GAGGTTACAG ATGACTCACC AAAATACAAC 
TATGCTTCAG ATCCAAGCGG TACCTATGAT TTCGGGAGCC TCGTGGCCAC AGTGAAAGCC 
ATCCGCAGGG AGAAGAGGTT TGTGGAGGAG GCATCCACTG GCCAGGAATG CGGGATAGTG 
CTGGACCGGA CCTGCTTCTA CGCCGAGCAG GGTGGGCAGA TCTATGACCA GGGCTACATG 
GTCAAGGATG ACGACAGCAG GGAGGATAAA ACAGAATTCA CTGTGAAGAA CGTGCAGGTC 
CGAGGAGGCT ACGTGCTCCA CATAGGAACT CTGTATGGAA GCTTGAAAGT AGGAGATCAG 
GTCCACCTGA CTATTGATGA GGCCAGGCGG AGGCCGGTCA TGAGCAACCA CACGGCCACC 
CACATCCTCA ACTTTGCCCT GCGCTCTGTG CTGGGGGAGG CTGACCAGAG GGGGTCCCTG 
GTGGCACCTG ACAGGCTGCG CTTCGACTTC ACAGCCAAGG GAGCCCTGTC CACTCAGGAA 
ATCAAGAAAG TTGAAGGGAT TGCCAACCAG ATGATTGAGG AGTCAAAGAC AGTGTATGCC 
AAGGACTGCC CTCTTGCAGC AGCCAAAGCT ATCCAAGGAC TTCGGGCAGT TTTTGATGAG 
ACCTACCCCG ATCCTGTCCG TGTGGTCTCC ATTGGCATCC CTGTGGAGGA GCTGCTGGCT 
GACCCCTCTG GCCCTGGGGG CTCCATCACC TCTATTGAGT TCTGCGGAGG GACGCACTTG 
CAGAACTCCA GCCATGCTGG CCCCTTTGTG ATTGTTTCAG AAGAGGCAAT TGCTAAAGGC 
ATTCGGAGGA TAGTTGCAGT CACTGGGGCT GAAGCTCGGA AGGCCCTCAG GAAAGTTGAC 
AGCCTCAGGA AGCTGCTGTC AGCCCTGGAG GCCAAGGTGA AGGTCCAGAC AGCTCCCAAC 
AAGGATGTGC AGAAGGAGAT CACGGATCTC GGTGAGACAC TGGCCACTGC TGTTATCCCA 
CAGTGGCAGA AAGATGAACT GAGAGAGGCT GTTAAAGCAC TGAAGAAAGT CATGGATGAC 
CTGGACCGAG CAAGCAAAGC CGATATCCAG AAAAGAGTAC TGGAAAAGAC CAAGCAAGTT 
ATTGAGAGTC ACCCTAACCA GCCATGGGTC ATCATGGAGA TGGAAAATGG AGCTTCAGCA 
AAGGCCCTGA ATGAATCTCT CAAGCTCTTC AAGACTAATT CCCCCCAAAC TGCTGCTATG 
CTTTTTGCTG TGGATAATGA AGCTGGCAGG ATCACCTGCC TGTGTCAAGT ACCCCAGGAG 
ACTGCAAATA AGGGCCTGAA GGCCAGCCAG TGGGTGCAGG AAGTGTCTGG CTTGATGGAC 
GGCAAAGGCG GTGGAAAGGA CATCTCTGCA CAGGCAACAG GCAAGAATGT GGGCTGCCTG 
CAGGAAGCCC TGAAACTGGC CACGGACTTT GCCAGGCTCC GCCTGGGGGA GCTGAAGAAC 
TGA