KIAA1257 cDNA ORF clone, Protobothrops mucrosquamatus

Gene
Clones
gRNAs

Summary

Gene Symbol	KIAA1257
Entrez Gene ID	107287869
Full Name	KIAA1257 ortholog
Gene Type	protein-coding
Organism	Protobothrops mucrosquamatus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015816423.1	XP_015671909.1	uncharacterized protein KIAA1257 homolog
XM_029284229.1	XP_029140062.1	uncharacterized protein KIAA1257 homolog

Pathways from BioSystems
Gene Ontology

The following KIAA1257 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIAA1257 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPr199182	XM_029284229.1 Latest version!	Protobothrops mucrosquamatus KIAA1257 ortholog (KIAA1257), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPr30606	XM_015816423.1 Latest version!	Protobothrops mucrosquamatus KIAA1257 ortholog (KIAA1257), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KIAA1257 ( XM_029284229.1 ) cDNA ORF clone, Protobothrops mucrosquamatus -> XP_029140062.1 Protobothrops mucrosquamatus KIAA1257 ortholog (KIAA1257), mRNA.

Price & Availability↑

CloneID	OPr199182
Clone ID Related Accession (Same CDS sequence)	XM_029284229.1
Accession Version	XM_029284229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-22
Organism	Protobothrops mucrosquamatus
Product	uncharacterized protein KIAA1257 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015386772.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Protobothrops mucrosquamatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGTT TTTATCATAT GGAGTATTTT CTTCTACCAG ATGATATTGA ACCTCGAAAA 
TTAGATTTGG TATTGTTTGG TCCAGTGGCT AAATTATTTC TGGATACAGA ATCTAAGGTA 
GTAAAACCAT GGCTTGAAAA TGACCAGATA TGGGTTTCAT GGAACCACAG CATTGAAATT 
AATATTACCA ATGAGTTTCT AATAAAACTA AGAGAACACA GCATAAAATT AAGGTTATGG 
GATCTCAAGG AAAAGGTTTG TTCAAAAGCC AGGTTTTGTA AACTAAAGAG TAATGTACCA 
CTATCAGATC AAGGAGAATT TGAAGGTAAT GTCAAGAATA TGGTTTTGTA TCAGAGAGAA 
TTCTTACAAC GTAATGAAGC CAAACCCAGT ATCACAAAGG TTAAACCACT AGCACCTTTT 
GTGCGAAAAC AGACAGATGC TTTGTCAGCT GTGAGCAAGC GCATGGAAAA TGACTCCAAG 
GCTAGTGATC AAAAATACTC TCGGAAATTT AGTTTTCTAC AACCTCCTGT AAAATCTGAT 
GAATCGAACC GTTCACTGAG CACACTCAGT ATTTCTGGGA GTATAGCGTC TTCTTTTAAA 
GAAAAAACCA TTCAGGAGGG TAAAACGATA AAACAGAAAC ACCGTGCATC ACTGTTAATA 
AAAACCAATG GGGGTAAATC GCCAATTTCT TCAGACAGAA AGGAAAGAGC GTCTATAGCT 
TTTCTTCACT CAGAAAAGAG GAGGCCATCG CAAAAAGGTA ACGTTTTCAG TGCTGACAAG 
AAATCAAAAC AGGCCGCCCA GAGGTCAGCT GGTAAAGAAG CCGCTGCCTT GGCAGCCCAG 
GCAAGGAAGT TTGGAATAGC GGTTTTGCAA CTGGACCTCA TGCCTCTTCT AGCAGGTGAA 
TACTATATAG CCAGTCGATG GGAAGAGAAT TCCCCCAAAC TTAATGAAGC ATACTTAGGT 
TTTGCTATAG ACAAACCTAT TATGAGTGAA AAACACAAAC ATGACTTAAA TCCACTGATT 
ATTAAGATTA AGTCTGCAAA ATGTCTACCA AATACTCCTG TTCCAATTGA AGAGTTGCAG 
AGAACCTGCA CTCCTGTTTA CTGCAAATAC ACTTTCTACA ATATGTGTCC CCATTATACA 
CAAGGTCGAG ATCATGGAAC TGATGTCTTC TTTAAAGATA TCAATGTGAT TCTTGTAGGA 
ACAATAGACC CTGGAAAATT CAGTGAATAC CTAAGAGGTC CAGACTTAGA AATTGAAGTT 
CATGACCGAG ACAAGAAAAT GGAAGATCTC AAGATGAAGC CATCTTTGTT TGGAGAAGAA 
CCAGGAGACA GCAAGCTAAG TGACATAAGC AATGTCACTT CGAGATACAT GCTCCAGAAT 
CCCATTACAG CCAAAGAAAA TATTTGTAGC CTGTATGGAG TAACTAAAAT CAATTTGAGC 
AATTTGTTGC TAGGGGAAAA GACCTTGAAT TTCTGTGCAC CAATTCACAA CAGCTCAGTT 
CAAGATACAT CTCTATTCCA TGGACAAAAC ACCAGTCCAG GGGATAAGAA AACAGATGAC 
TCTCAGGTTG TTCAATTTCC AAATGGGAAT TATGTAAATG CTGAGTCATA TCTTAAAGTG 
AGAGTTGAAA TAAGTGTACC ATTAGGTCCT TCAGAGGAAG AGGGCGCTGC TGCACGTTGT 
CCATATGGCT GCATCATTTA TCTTTTTGAT TATAATAATA CATCATTCTT ACACTATTTG 
ATGCAGGAGA TTACAGAAAT TAATGCAGAG GCTTTCCTAC TTGATTCCTA TCCCATAGAT 
ACAACTCAAA AATCTCTTAA TACTTTGAAA CTGAATCACA AACTTTCCTT AGAAGAGATT 
TCTCGGCTAA ATATTATTAC TGGGTTTCAC ATAATTGACG GGTCCATTCA CCTTCTGATC 
CTGGAAGGTT TCAGAAATAA AGCACTTAAG AGGATGTGGA ATAAAAAAAT TGATAGGGTG 
CAGGAAACGG AACGTGGGAG ATTACAGATT TTCTATAATT CACACCTGTC TTTTCACAAG 
CGGCTGTATA TAGATCTTGA AGCTATCCTG CTTCACATCC GTCTCTGCAA GCCACTGTCA 
AGCATCATGA AGCAACCTTT GTTATATATC AGGGATATGG TTCCTCAGCC GTGTTTTGAA 
GCTCTTTCCA GACTTGATTA CATCTGCCAC AGTAAGAAGT TAAGGGAAGT GGTCCATTAC 
AGTCTCTTGC CTTCAGCAGA AATGATCACA ATGTTGAGTC AGGAGTTTGG GGTTCCCTTG 
ACTAAAGCTG ACATGTATGC AAGGGAGCCC CCAGAGATCT TGGAATCAGT CAGTAAAATG 
CAAAAGGATC CAAGCATGAG GCAGTATGGG TGTTCTCACT TGGATAACCA CAACGAGAAG 
TATGTACTGA GAAAAAAGGA ACAAGAAAGC CAGGCACCAC ATAATTATAT CCAGTCCAAT 
ATTGAATATG TGAACCTCCT CAATGAGATA TTTGAAAGAG ACATCCCGAG AACTATCAGA 
GCTTTCCCTT TTGGTGAAAA TGCTATCTTT AACTATAGTT GTCAGACATT AAATTCAGCA 
GAAATTGCAA AGAAGCTACT TCGACAAGAA ATGGCAAAGA TGCCAGGAAA AAGATTTGCT 
TATAATGAAT ATCTGTCAGG TATGTTTGAC CCAGTGGATG AGGACAGTAT ATTAAAAGAA 
CGTATTCAAC TATCAAAGAA ATTGTGGTTG TATCCTGGAG GGTTTGCAAA TCCTGGATTT 
AAGAGCAGCG CAGACTCCAA CAAACATCCC CGTAGGCCTG ATGAGGCACG AGTGATAGAA 
TTAAGAGAGA AATGGCAGGA GAATTTTTTC AATGCTAGTT TTATGAAGCC AGTTCTGGAT 
CGGGATAGAT GGAGATGGGA CAAACGATAT GCTGATTTTG AGCTGTACCA AAAGCCTGTA 
GTCCAAAACC CGGTTCCTGA CCCTTGTAGA GAAGAGTCTA AGATACAAGC GTCCTTTAAT 
ACTGCGCTGA AAGTTCATCG GTGCTGCCCA GCAACAGAGC TTATTTCTAG TGGACCCAAA 
GCCAGCTGCC AAGTCGCTCG CCTTCGGGGT CTGCTGAAGG ATAAACCAGT CAAGTTATCT 
CTAAAGATGC AGTCTCTTCC AGTTCTGGGA GTTTTAGGTA ACATGGAAGA GTGCAGTCCC 
TGTAAAGGAT TTGTTCCTGG TGCTGACATT CACCACAGCT TGATGTCAAG CCACAATATT 
CCGTGCTATG ACAGGGCACA CGATGTATTT AAAACTCTGA AAGGAGCAGA TTTCAGACTG 
ATTTGTCAGA ATCGTTCATA TCATTACAAG AGGTATTCAT TTTTGAAGAA ACACATCTCA 
GCAATAGATA AAACAGAGAA TGAAGTGCCT GTTGCCTCAC AGCTGAATAA CCCCATGACT 
GAAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029140062.1
CDS	91..3519
Translation	MHRFYHMEYFLLPDDIEPRKLDLVLFGPVAKLFLDTESKVVKPWLENDQIWVSWNHSIEINITNEFLIKLREHSIKLRLWDLKEKVCSKARFCKLKSNVPLSDQGEFEGNVKNMVLYQREFLQRNEAKPSITKVKPLAPFVRKQTDALSAVSKRMENDSKASDQKYSRKFSFLQPPVKSDESNRSLSTLSISGSIASSFKEKTIQEGKTIKQKHRASLLIKTNGGKSPISSDRKERASIAFLHSEKRRPSQKGNVFSADKKSKQAAQRSAGKEAAALAAQARKFGIAVLQLDLMPLLAGEYYIASRWEENSPKLNEAYLGFAIDKPIMSEKHKHDLNPLIIKIKSAKCLPNTPVPIEELQRTCTPVYCKYTFYNMCPHYTQGRDHGTDVFFKDINVILVGTIDPGKFSEYLRGPDLEIEVHDRDKKMEDLKMKPSLFGEEPGDSKLSDISNVTSRYMLQNPITAKENICSLYGVTKINLSNLLLGEKTLNFCAPIHNSSVQDTSLFHGQNTSPGDKKTDDSQVVQFPNGNYVNAESYLKVRVEISVPLGPSEEEGAAARCPYGCIIYLFDYNNTSFLHYLMQEITEINAEAFLLDSYPIDTTQKSLNTLKLNHKLSLEEISRLNIITGFHIIDGSIHLLILEGFRNKALKRMWNKKIDRVQETERGRLQIFYNSHLSFHKRLYIDLEAILLHIRLCKPLSSIMKQPLLYIRDMVPQPCFEALSRLDYICHSKKLREVVHYSLLPSAEMITMLSQEFGVPLTKADMYAREPPEILESVSKMQKDPSMRQYGCSHLDNHNEKYVLRKKEQESQAPHNYIQSNIEYVNLLNEIFERDIPRTIRAFPFGENAIFNYSCQTLNSAEIAKKLLRQEMAKMPGKRFAYNEYLSGMFDPVDEDSILKERIQLSKKLWLYPGGFANPGFKSSADSNKHPRRPDEARVIELREKWQENFFNASFMKPVLDRDRWRWDKRYADFELYQKPVVQNPVPDPCREESKIQASFNTALKVHRCCPATELISSGPKASCQVARLRGLLKDKPVKLSLKMQSLPVLGVLGNMEECSPCKGFVPGADIHHSLMSSHNIPCYDRAHDVFKTLKGADFRLICQNRSYHYKRYSFLKKHISAIDKTENEVPVASQLNNPMTEI

Target ORF information:

RefSeq Version	XM_029284229.1
Organism	Protobothrops mucrosquamatus
Definition	Protobothrops mucrosquamatus KIAA1257 ortholog (KIAA1257), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029284229.1

ORF Insert Sequence:

ATGCATCGTT TTTATCATAT GGAGTATTTT CTTCTACCAG ATGATATTGA ACCTCGAAAA 
TTAGATTTGG TATTGTTTGG TCCAGTGGCT AAATTATTTC TGGATACAGA ATCTAAGGTA 
GTAAAACCAT GGCTTGAAAA TGACCAGATA TGGGTTTCAT GGAACCACAG CATTGAAATT 
AATATTACCA ATGAGTTTCT AATAAAACTA AGAGAACACA GCATAAAATT AAGGTTATGG 
GATCTCAAGG AAAAGGTTTG TTCAAAAGCC AGGTTTTGTA AACTAAAGAG TAATGTACCA 
CTATCAGATC AAGGAGAATT TGAAGGTAAT GTCAAGAATA TGGTTTTGTA TCAGAGAGAA 
TTCTTACAAC GTAATGAAGC CAAACCCAGT ATCACAAAGG TTAAACCACT AGCACCTTTT 
GTGCGAAAAC AGACAGATGC TTTGTCAGCT GTGAGCAAGC GCATGGAAAA TGACTCCAAG 
GCTAGTGATC AAAAATACTC TCGGAAATTT AGTTTTCTAC AACCTCCTGT AAAATCTGAT 
GAATCGAACC GTTCACTGAG CACACTCAGT ATTTCTGGGA GTATAGCGTC TTCTTTTAAA 
GAAAAAACCA TTCAGGAGGG TAAAACGATA AAACAGAAAC ACCGTGCATC ACTGTTAATA 
AAAACCAATG GGGGTAAATC GCCAATTTCT TCAGACAGAA AGGAAAGAGC GTCTATAGCT 
TTTCTTCACT CAGAAAAGAG GAGGCCATCG CAAAAAGGTA ACGTTTTCAG TGCTGACAAG 
AAATCAAAAC AGGCCGCCCA GAGGTCAGCT GGTAAAGAAG CCGCTGCCTT GGCAGCCCAG 
GCAAGGAAGT TTGGAATAGC GGTTTTGCAA CTGGACCTCA TGCCTCTTCT AGCAGGTGAA 
TACTATATAG CCAGTCGATG GGAAGAGAAT TCCCCCAAAC TTAATGAAGC ATACTTAGGT 
TTTGCTATAG ACAAACCTAT TATGAGTGAA AAACACAAAC ATGACTTAAA TCCACTGATT 
ATTAAGATTA AGTCTGCAAA ATGTCTACCA AATACTCCTG TTCCAATTGA AGAGTTGCAG 
AGAACCTGCA CTCCTGTTTA CTGCAAATAC ACTTTCTACA ATATGTGTCC CCATTATACA 
CAAGGTCGAG ATCATGGAAC TGATGTCTTC TTTAAAGATA TCAATGTGAT TCTTGTAGGA 
ACAATAGACC CTGGAAAATT CAGTGAATAC CTAAGAGGTC CAGACTTAGA AATTGAAGTT 
CATGACCGAG ACAAGAAAAT GGAAGATCTC AAGATGAAGC CATCTTTGTT TGGAGAAGAA 
CCAGGAGACA GCAAGCTAAG TGACATAAGC AATGTCACTT CGAGATACAT GCTCCAGAAT 
CCCATTACAG CCAAAGAAAA TATTTGTAGC CTGTATGGAG TAACTAAAAT CAATTTGAGC 
AATTTGTTGC TAGGGGAAAA GACCTTGAAT TTCTGTGCAC CAATTCACAA CAGCTCAGTT 
CAAGATACAT CTCTATTCCA TGGACAAAAC ACCAGTCCAG GGGATAAGAA AACAGATGAC 
TCTCAGGTTG TTCAATTTCC AAATGGGAAT TATGTAAATG CTGAGTCATA TCTTAAAGTG 
AGAGTTGAAA TAAGTGTACC ATTAGGTCCT TCAGAGGAAG AGGGCGCTGC TGCACGTTGT 
CCATATGGCT GCATCATTTA TCTTTTTGAT TATAATAATA CATCATTCTT ACACTATTTG 
ATGCAGGAGA TTACAGAAAT TAATGCAGAG GCTTTCCTAC TTGATTCCTA TCCCATAGAT 
ACAACTCAAA AATCTCTTAA TACTTTGAAA CTGAATCACA AACTTTCCTT AGAAGAGATT 
TCTCGGCTAA ATATTATTAC TGGGTTTCAC ATAATTGACG GGTCCATTCA CCTTCTGATC 
CTGGAAGGTT TCAGAAATAA AGCACTTAAG AGGATGTGGA ATAAAAAAAT TGATAGGGTG 
CAGGAAACGG AACGTGGGAG ATTACAGATT TTCTATAATT CACACCTGTC TTTTCACAAG 
CGGCTGTATA TAGATCTTGA AGCTATCCTG CTTCACATCC GTCTCTGCAA GCCACTGTCA 
AGCATCATGA AGCAACCTTT GTTATATATC AGGGATATGG TTCCTCAGCC GTGTTTTGAA 
GCTCTTTCCA GACTTGATTA CATCTGCCAC AGTAAGAAGT TAAGGGAAGT GGTCCATTAC 
AGTCTCTTGC CTTCAGCAGA AATGATCACA ATGTTGAGTC AGGAGTTTGG GGTTCCCTTG 
ACTAAAGCTG ACATGTATGC AAGGGAGCCC CCAGAGATCT TGGAATCAGT CAGTAAAATG 
CAAAAGGATC CAAGCATGAG GCAGTATGGG TGTTCTCACT TGGATAACCA CAACGAGAAG 
TATGTACTGA GAAAAAAGGA ACAAGAAAGC CAGGCACCAC ATAATTATAT CCAGTCCAAT 
ATTGAATATG TGAACCTCCT CAATGAGATA TTTGAAAGAG ACATCCCGAG AACTATCAGA 
GCTTTCCCTT TTGGTGAAAA TGCTATCTTT AACTATAGTT GTCAGACATT AAATTCAGCA 
GAAATTGCAA AGAAGCTACT TCGACAAGAA ATGGCAAAGA TGCCAGGAAA AAGATTTGCT 
TATAATGAAT ATCTGTCAGG TATGTTTGAC CCAGTGGATG AGGACAGTAT ATTAAAAGAA 
CGTATTCAAC TATCAAAGAA ATTGTGGTTG TATCCTGGAG GGTTTGCAAA TCCTGGATTT 
AAGAGCAGCG CAGACTCCAA CAAACATCCC CGTAGGCCTG ATGAGGCACG AGTGATAGAA 
TTAAGAGAGA AATGGCAGGA GAATTTTTTC AATGCTAGTT TTATGAAGCC AGTTCTGGAT 
CGGGATAGAT GGAGATGGGA CAAACGATAT GCTGATTTTG AGCTGTACCA AAAGCCTGTA 
GTCCAAAACC CGGTTCCTGA CCCTTGTAGA GAAGAGTCTA AGATACAAGC GTCCTTTAAT 
ACTGCGCTGA AAGTTCATCG GTGCTGCCCA GCAACAGAGC TTATTTCTAG TGGACCCAAA 
GCCAGCTGCC AAGTCGCTCG CCTTCGGGGT CTGCTGAAGG ATAAACCAGT CAAGTTATCT 
CTAAAGATGC AGTCTCTTCC AGTTCTGGGA GTTTTAGGTA ACATGGAAGA GTGCAGTCCC 
TGTAAAGGAT TTGTTCCTGG TGCTGACATT CACCACAGCT TGATGTCAAG CCACAATATT 
CCGTGCTATG ACAGGGCACA CGATGTATTT AAAACTCTGA AAGGAGCAGA TTTCAGACTG 
ATTTGTCAGA ATCGTTCATA TCATTACAAG AGGTATTCAT TTTTGAAGAA ACACATCTCA 
GCAATAGATA AAACAGAGAA TGAAGTGCCT GTTGCCTCAC AGCTGAATAA CCCCATGACT 
GAAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1257 ( XM_015816423.1 ) cDNA ORF clone, Protobothrops mucrosquamatus -> XP_015671909.1 Protobothrops mucrosquamatus KIAA1257 ortholog (KIAA1257), mRNA.

Price & Availability↑

CloneID	OPr30606
Clone ID Related Accession (Same CDS sequence)	XM_015816423.1
Accession Version	XM_015816423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-01
Organism	Protobothrops mucrosquamatus
Product	uncharacterized protein KIAA1257 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015386772.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Protobothrops mucrosquamatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 19% of CDS bases ##RefSeq-Attributes-END##

ATGCATCGTT TTTATCATAT GGAGTATTTT CTTCTACCAG ATGATATTGA ACCTCGAAAA 
TTAGATTTGG TATTGTTTGG TCCAGTGGCT AAATTATTTC TGGATACAGA ATCTAAGGTA 
GTAAAACCAT GGCTTGAAAA TGACCAGATA TGGGTTTCAT GGAACCACAG CATTGAAATT 
AATATTACCA ATGAGTTTCT AATAAAACTA AGAGAACACA GCATAAAATT AAGGTTATGG 
GATCTCAAGG AAAAGGTTTG TTCAAAAGCC AGGTTTTGTA AACTAAAGAG TAATGTACCA 
CTATCAGATC AAGGAGAATT TGAAGGTAAT GTCAAGAATA TGGTTTTGTA TCAGAGAGAA 
TTCTTACAAC GTAATGAAGC CAAACCCAGT ATCACAAAGG TTAAACCACT AGCACCTTTT 
GTGCGAAAAC AGACAGATGC TTTGTCAGCT GTGAGCAAGC GCATGGAAAA TGACTCCAAG 
GCTAGTGATC AAAAATACTC TCGGAAATTT AGTTTTCTAC AACCTCCTGT AAAATCTGAT 
GAATCGAACC GTTCACTGAG CACACTCAGT ATTTCTGGGA GTATAGCGTC TTCTTTTAAA 
GAAAAAACCA TTCAGGAGGG TAAAACGATA AAACAGAAAC ACCGTGCATC ACTGTTAATA 
AAAACCAATG GGGGTAAATC GCCAATTTCT TCAGACAGAA AGGAAAGAGC GTCTATAGCT 
TTTCTTCACT CAGAAAAGAG GAGGCCATCG CAAAAAGGTA ACGTTTTCAG TGCTGACAAG 
AAATCAAAAC AGGCCGCCCA GAGGTCAGCT GGTAAAGAAG CCGCTGCCTT GGCAGCCCAG 
GCAAGGAAGT TTGGAATAGC GGTTTTGCAA CTGGACCTCA TGCCTCTTCT AGCAGGTGAA 
TACTATATAG CCAGTCGATG GGAAGAGAAT TCCCCCAAAC TTAATGAAGC ATACTTAGGT 
TTTGCTATAG ACAAACCTAT TATGAGTGAA AAACACAAAC ATGACTTAAA TCCACTGATT 
ATTAAGATTA AGTCTGCAAA ATGTCTACCA AATACTCCTG TTCCAATTGA AGAGTTGCAG 
AGAACCTGCA CTCCTGTTTA CTGCAAATAC ACTTTCTACA ATATGTGTCC CCATTATACA 
CAAGGTCGAG ATCATGGAAC TGATGTCTTC TTTAAAGATA TCAATGTGAT TCTTGTAGGA 
ACAATAGACC CTGGAAAATT CAGTGAATAC CTAAGAGGTC CAGACTTAGA AATTGAAGTT 
CATGACCGAG ACAAGAAAAT GGAAGATCTC AAGATGAAGC CATCTTTGTT TGGAGAAGAA 
CCAGGAGACA GCAAGCTAAG TGACATAAGC AATGTCACTT CGAGATACAT GCTCCAGAAT 
CCCATTACAG CCAAAGAAAA TATTTGTAGC CTGTATGGAG TAACTAAAAT CAATTTGAGC 
AATTTGTTGC TAGGGGAAAA GACCTTGAAT TTCTGTGCAC CAATTCACAA CAGCTCAGTT 
CAAGATACAT CTCTATTCCA TGGACAAAAC ACCAGTCCAG GGGATAAGAA AACAGATGAC 
TCTCAGGTTG TTCAATTTCC AAATGGGAAT TATGTAAATG CTGAGTCATA TCTTAAAGTG 
AGAGTTGAAA TAAGTGTACC ATTAGGTCCT TCAGAGGAAG AGGGCGCTGC TGCACGTTGT 
CCATATGGCT GCATCATTTA TCTTTTTGAT TATAATAATA CATCATTCTT ACACTATTTG 
ATGCAGGAGA TTACAGAAAT TAATGCAGAG GCTTTCCTAC TTGATTCCTA TCCCATAGAT 
ACAACTCAAA AATCTCTTAA TACTTTGAAA CTGAATCACA AACTTTCCTT AGAAGAGATT 
TCTCGGCTAA ATATTATTAC TGGGTTTCAC ATAATTGACG GGTCCATTCA CCTTCTGATC 
CTGGAAGGTT TCAGAAATAA AGCACTTAAG AGGATGTGGA ATAAAAAAAT TGATAGGGTG 
CAGGAAACGG AACGTGGGAG ATTACAGATT TTCTATAATT CACACCTGTC TTTTCACAAG 
CGGCTGTATA TAGATCTTGA AGCTATCCTG CTTCACATCC GTCTCTGCAA GCCACTGTCA 
AGCATCATGA AGCAACCTTT GTTATATATC AGGGATATGG TTCCTCAGCC GTGTTTTGAA 
GCTCTTTCCA GACTTGATTA CATCTGCCAC AGTAAGAAGT TAAGGGAAGT GGTCCATTAC 
AGTCTCTTGC CTTCAGCAGA AATGATCACA ATGTTGAGTC AGGAGTTTGG GGTTCCCTTG 
ACTAAAGCTG ACATGTATGC AAGGGAGCCC CCAGAGATCT TGGAATCAGT CAGTAAAATG 
CAAAAGGATC CAAGCATGAG GCAGTATGGG TGTTCTCACT TGGATAACCA CAACGAGAAG 
TATGTACTGA GAAAAAAGGA ACAAGAAAGC CAGGCACCAC ATAATTATAT CCAGTCCAAT 
ATTGAATATG TGAACCTCCT CAATGAGATA TTTGAAAGAG ACATCCCGAG AACTATCAGA 
GCTTTCCCTT TTGGTGAAAA TGCTATCTTT AACTATAGTT GTCAGACATT AAATTCAGCA 
GAAATTGCAA AGAAGCTACT TCGACAAGAA ATGGCAAAGA TGCCAGGAAA AAGATTTGCT 
TATAATGAAT ATCTGTCAGG TATGTTTGAC CCAGTGGATG AGGACAGTAT ATTAAAAGAA 
CGTATTCAAC TATCAAAGAA ATTGTGGTTG TATCCTGGAG GGTTTGCAAA TCCTGGATTT 
AAGAGCAGCG CAGACTCCAA CAAACATCCC CGTAGGCCTG ATGAGGCACG AGTGATAGAA 
TTAAGAGAGT CTCTTCCAGT TCTGGGAGTT TTAGGTAACA TGGAAGAGTG CAGTCCCTGT 
AAAGGATTTG TTCCTGGTGC TGACATTCAC CACAGCTTGA TGTCAAGCCA CAATATTCCG 
TGCTATGACA GGGCACACGA TGTATTTAAA ACTCTGAAAG GAGCAGATTT CAGCCAGAGC 
ATACGTCTTC CAGTGATGTT GGACGCCATA GCCCTGGAAG AAAATCTAGC GGCCTGCAAA 
GATGCGTCTG ATGCATATTC AGTGGCTGCC ACCATTTTGC TGAGATCCAA ATGTGTGCGG 
ACATCCTCCG CTGGAACCCG GGCTTGGAGC TCCCGGAGCC AGGTGAGGGC GGCCCTGGTG 
AAAAAAGAGG CCGAAGTTGC GGTCCTGATC GCCCAGGCAG CCGCCTGGTG CGTTCTCTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015671909.1
CDS	213..3455
Translation	MHRFYHMEYFLLPDDIEPRKLDLVLFGPVAKLFLDTESKVVKPWLENDQIWVSWNHSIEINITNEFLIKLREHSIKLRLWDLKEKVCSKARFCKLKSNVPLSDQGEFEGNVKNMVLYQREFLQRNEAKPSITKVKPLAPFVRKQTDALSAVSKRMENDSKASDQKYSRKFSFLQPPVKSDESNRSLSTLSISGSIASSFKEKTIQEGKTIKQKHRASLLIKTNGGKSPISSDRKERASIAFLHSEKRRPSQKGNVFSADKKSKQAAQRSAGKEAAALAAQARKFGIAVLQLDLMPLLAGEYYIASRWEENSPKLNEAYLGFAIDKPIMSEKHKHDLNPLIIKIKSAKCLPNTPVPIEELQRTCTPVYCKYTFYNMCPHYTQGRDHGTDVFFKDINVILVGTIDPGKFSEYLRGPDLEIEVHDRDKKMEDLKMKPSLFGEEPGDSKLSDISNVTSRYMLQNPITAKENICSLYGVTKINLSNLLLGEKTLNFCAPIHNSSVQDTSLFHGQNTSPGDKKTDDSQVVQFPNGNYVNAESYLKVRVEISVPLGPSEEEGAAARCPYGCIIYLFDYNNTSFLHYLMQEITEINAEAFLLDSYPIDTTQKSLNTLKLNHKLSLEEISRLNIITGFHIIDGSIHLLILEGFRNKALKRMWNKKIDRVQETERGRLQIFYNSHLSFHKRLYIDLEAILLHIRLCKPLSSIMKQPLLYIRDMVPQPCFEALSRLDYICHSKKLREVVHYSLLPSAEMITMLSQEFGVPLTKADMYAREPPEILESVSKMQKDPSMRQYGCSHLDNHNEKYVLRKKEQESQAPHNYIQSNIEYVNLLNEIFERDIPRTIRAFPFGENAIFNYSCQTLNSAEIAKKLLRQEMAKMPGKRFAYNEYLSGMFDPVDEDSILKERIQLSKKLWLYPGGFANPGFKSSADSNKHPRRPDEARVIELRESLPVLGVLGNMEECSPCKGFVPGADIHHSLMSSHNIPCYDRAHDVFKTLKGADFSQSIRLPVMLDAIALEENLAACKDASDAYSVAATILLRSKCVRTSSAGTRAWSSRSQVRAALVKKEAEVAVLIAQAAAWCVLL

Target ORF information:

RefSeq Version	XM_015816423.1
Organism	Protobothrops mucrosquamatus
Definition	Protobothrops mucrosquamatus KIAA1257 ortholog (KIAA1257), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015816423.1

ORF Insert Sequence:

ATGCATCGTT TTTATCATAT GGAGTATTTT CTTCTACCAG ATGATATTGA ACCTCGAAAA 
TTAGATTTGG TATTGTTTGG TCCAGTGGCT AAATTATTTC TGGATACAGA ATCTAAGGTA 
GTAAAACCAT GGCTTGAAAA TGACCAGATA TGGGTTTCAT GGAACCACAG CATTGAAATT 
AATATTACCA ATGAGTTTCT AATAAAACTA AGAGAACACA GCATAAAATT AAGGTTATGG 
GATCTCAAGG AAAAGGTTTG TTCAAAAGCC AGGTTTTGTA AACTAAAGAG TAATGTACCA 
CTATCAGATC AAGGAGAATT TGAAGGTAAT GTCAAGAATA TGGTTTTGTA TCAGAGAGAA 
TTCTTACAAC GTAATGAAGC CAAACCCAGT ATCACAAAGG TTAAACCACT AGCACCTTTT 
GTGCGAAAAC AGACAGATGC TTTGTCAGCT GTGAGCAAGC GCATGGAAAA TGACTCCAAG 
GCTAGTGATC AAAAATACTC TCGGAAATTT AGTTTTCTAC AACCTCCTGT AAAATCTGAT 
GAATCGAACC GTTCACTGAG CACACTCAGT ATTTCTGGGA GTATAGCGTC TTCTTTTAAA 
GAAAAAACCA TTCAGGAGGG TAAAACGATA AAACAGAAAC ACCGTGCATC ACTGTTAATA 
AAAACCAATG GGGGTAAATC GCCAATTTCT TCAGACAGAA AGGAAAGAGC GTCTATAGCT 
TTTCTTCACT CAGAAAAGAG GAGGCCATCG CAAAAAGGTA ACGTTTTCAG TGCTGACAAG 
AAATCAAAAC AGGCCGCCCA GAGGTCAGCT GGTAAAGAAG CCGCTGCCTT GGCAGCCCAG 
GCAAGGAAGT TTGGAATAGC GGTTTTGCAA CTGGACCTCA TGCCTCTTCT AGCAGGTGAA 
TACTATATAG CCAGTCGATG GGAAGAGAAT TCCCCCAAAC TTAATGAAGC ATACTTAGGT 
TTTGCTATAG ACAAACCTAT TATGAGTGAA AAACACAAAC ATGACTTAAA TCCACTGATT 
ATTAAGATTA AGTCTGCAAA ATGTCTACCA AATACTCCTG TTCCAATTGA AGAGTTGCAG 
AGAACCTGCA CTCCTGTTTA CTGCAAATAC ACTTTCTACA ATATGTGTCC CCATTATACA 
CAAGGTCGAG ATCATGGAAC TGATGTCTTC TTTAAAGATA TCAATGTGAT TCTTGTAGGA 
ACAATAGACC CTGGAAAATT CAGTGAATAC CTAAGAGGTC CAGACTTAGA AATTGAAGTT 
CATGACCGAG ACAAGAAAAT GGAAGATCTC AAGATGAAGC CATCTTTGTT TGGAGAAGAA 
CCAGGAGACA GCAAGCTAAG TGACATAAGC AATGTCACTT CGAGATACAT GCTCCAGAAT 
CCCATTACAG CCAAAGAAAA TATTTGTAGC CTGTATGGAG TAACTAAAAT CAATTTGAGC 
AATTTGTTGC TAGGGGAAAA GACCTTGAAT TTCTGTGCAC CAATTCACAA CAGCTCAGTT 
CAAGATACAT CTCTATTCCA TGGACAAAAC ACCAGTCCAG GGGATAAGAA AACAGATGAC 
TCTCAGGTTG TTCAATTTCC AAATGGGAAT TATGTAAATG CTGAGTCATA TCTTAAAGTG 
AGAGTTGAAA TAAGTGTACC ATTAGGTCCT TCAGAGGAAG AGGGCGCTGC TGCACGTTGT 
CCATATGGCT GCATCATTTA TCTTTTTGAT TATAATAATA CATCATTCTT ACACTATTTG 
ATGCAGGAGA TTACAGAAAT TAATGCAGAG GCTTTCCTAC TTGATTCCTA TCCCATAGAT 
ACAACTCAAA AATCTCTTAA TACTTTGAAA CTGAATCACA AACTTTCCTT AGAAGAGATT 
TCTCGGCTAA ATATTATTAC TGGGTTTCAC ATAATTGACG GGTCCATTCA CCTTCTGATC 
CTGGAAGGTT TCAGAAATAA AGCACTTAAG AGGATGTGGA ATAAAAAAAT TGATAGGGTG 
CAGGAAACGG AACGTGGGAG ATTACAGATT TTCTATAATT CACACCTGTC TTTTCACAAG 
CGGCTGTATA TAGATCTTGA AGCTATCCTG CTTCACATCC GTCTCTGCAA GCCACTGTCA 
AGCATCATGA AGCAACCTTT GTTATATATC AGGGATATGG TTCCTCAGCC GTGTTTTGAA 
GCTCTTTCCA GACTTGATTA CATCTGCCAC AGTAAGAAGT TAAGGGAAGT GGTCCATTAC 
AGTCTCTTGC CTTCAGCAGA AATGATCACA ATGTTGAGTC AGGAGTTTGG GGTTCCCTTG 
ACTAAAGCTG ACATGTATGC AAGGGAGCCC CCAGAGATCT TGGAATCAGT CAGTAAAATG 
CAAAAGGATC CAAGCATGAG GCAGTATGGG TGTTCTCACT TGGATAACCA CAACGAGAAG 
TATGTACTGA GAAAAAAGGA ACAAGAAAGC CAGGCACCAC ATAATTATAT CCAGTCCAAT 
ATTGAATATG TGAACCTCCT CAATGAGATA TTTGAAAGAG ACATCCCGAG AACTATCAGA 
GCTTTCCCTT TTGGTGAAAA TGCTATCTTT AACTATAGTT GTCAGACATT AAATTCAGCA 
GAAATTGCAA AGAAGCTACT TCGACAAGAA ATGGCAAAGA TGCCAGGAAA AAGATTTGCT 
TATAATGAAT ATCTGTCAGG TATGTTTGAC CCAGTGGATG AGGACAGTAT ATTAAAAGAA 
CGTATTCAAC TATCAAAGAA ATTGTGGTTG TATCCTGGAG GGTTTGCAAA TCCTGGATTT 
AAGAGCAGCG CAGACTCCAA CAAACATCCC CGTAGGCCTG ATGAGGCACG AGTGATAGAA 
TTAAGAGAGT CTCTTCCAGT TCTGGGAGTT TTAGGTAACA TGGAAGAGTG CAGTCCCTGT 
AAAGGATTTG TTCCTGGTGC TGACATTCAC CACAGCTTGA TGTCAAGCCA CAATATTCCG 
TGCTATGACA GGGCACACGA TGTATTTAAA ACTCTGAAAG GAGCAGATTT CAGCCAGAGC 
ATACGTCTTC CAGTGATGTT GGACGCCATA GCCCTGGAAG AAAATCTAGC GGCCTGCAAA 
GATGCGTCTG ATGCATATTC AGTGGCTGCC ACCATTTTGC TGAGATCCAA ATGTGTGCGG 
ACATCCTCCG CTGGAACCCG GGCTTGGAGC TCCCGGAGCC AGGTGAGGGC GGCCCTGGTG 
AAAAAAGAGG CCGAAGTTGC GGTCCTGATC GCCCAGGCAG CCGCCTGGTG CGTTCTCTTG 
TAG