ZNRF3 cDNA ORF clone, Protobothrops mucrosquamatus

Gene
Clones
gRNAs

Summary

Gene Symbol	ZNRF3
Entrez Gene ID	107287285
Full Name	zinc and ring finger 3
Gene Type	protein-coding
Organism	Protobothrops mucrosquamatus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015815778.1	XP_015671264.1	E3 ubiquitin-protein ligase ZNRF3
XM_015815778.2	XP_015671264.2	E3 ubiquitin-protein ligase ZNRF3

Pathways from BioSystems
Gene Ontology

The following ZNRF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZNRF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPr199046	XM_015815778.2 Latest version!	Protobothrops mucrosquamatus zinc and ring finger 3 (ZNRF3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OPr30060	XM_015815778.1 Latest version!	Protobothrops mucrosquamatus zinc and ring finger 3 (ZNRF3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZNRF3 ( XM_015815778.2 ) cDNA ORF clone, Protobothrops mucrosquamatus -> XP_015671264.2 Protobothrops mucrosquamatus zinc and ring finger 3 (ZNRF3), mRNA.

Price & Availability↑

CloneID	OPr199046
Clone ID Related Accession (Same CDS sequence)	XM_015815778.2
Accession Version	XM_015815778.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-22
Organism	Protobothrops mucrosquamatus
Product	E3 ubiquitin-protein ligase ZNRF3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015386706.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 23, 2019 this sequence version replaced XM_015815778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Protobothrops mucrosquamatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 22% of CDS bases ##RefSeq-Attributes-END##

ATGCATCCAT TGGGACTCTG CAACAGCAAT GATGAAGAAG ACTTATATGA ATATGGCTGG 
GTTGGAGTTG TGAAGCTGGA GCAGCCTGAC CTGGAACCAA AACCCTGCCT CACTGTTCTG 
GGCAAGGCCA AGCGGGCAGT TCAAAGAGGA GCCACAGCTG TCATTTTTGA TGTGTCCGAA 
AACCCAGATG CCATTGATCA GCTGAATCAG GGCTTGGAGG ACCCATTGAA AAGACCAGTT 
GTTTATGTGA AGGGGGCAGA TGCAGTCAAG CTGATGAACA TTGTTAACAA ACAGAAAGTG 
GCGCGGGCCA GAATCCAGCA CCGTCCACCA CCACAACCCA CCGAATACTT TGACATGGGG 
ATTTTCCTTG CCTTTTTTGT GGTGGTCTCT CTGGTTTGCC TGGTTCTTCT CATCAAGATC 
AAATTGAAGC AGCGTCGCAG CCAGAGCTCT ATGAACAGGC TGGCTGTGCA GGCGCTGGAA 
AAGATGGAGA CGTGGAAGTT CAAATCCAAG CTGAAAGGGC CCTGCGAAGG CAGCTGCGGT 
GCCCTGGACA CGCTCAGCAA CGGCTCTGCC TCGGACTGCG CTATTTGCTT GGAGAAGTAC 
CTGGATGGGG AGGAGCTGCG TGTGATTCCC TGCACCCACC GGTTCCACAG GAAGTGTGTG 
GATCCTTGGC TTCTGCAGCA TCACACTTGC CCCCACTGCC GTCACAACAT CATTGAGCAA 
AAGAAGGGAA ACGCCAGCCT GGTGTGTGAG GAGACGGCCA GCCAGTTACA CAGCCGGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CGCATGATCC TGCCTGTCCA CTTCCCTGGC 
CGAGCACACA GAGGTGGTCA CGTCACCGCC TACCCCACGC GCACCAGCAT GGACCCTCAC 
GGGAACCCCA TCACCGTCTT GACGGTTGAT CGGCATGGAG AGGCCCCGGC CTTCTTACGG 
AGCTACCCGC CCATCCTGCA CCTGGAGCCT CACCTGGGCA CAGCCGAACG CCAGCCCTGT 
ACAATGCCTC ATCCTTTCCA GAGGCCGAAG TTCAGCGGGG ACCCTTTCTC CAAGCCGGCT 
TGCTTCTCCC AGTACGAGAC CATGTTTCAG CACTACTACT TCCAGGGCCT CAGTTACCCT 
GAGCCGCCAC AGAGTGGACA GTTCCCCCTC CACGTGGCTG CCAAGGGCCT CCCCCGTGCC 
TTCCAGCCTC TCGGCAGCCA CAGGCTCTTC CCCCCTGCGG TCCATGTAGC CCCATCCTCC 
CAGCCAGAGA GTGGCAGTGC TTCTGGCTTC AGCTGTTACC GTGGCCACCG CTCGATCTGC 
AGCGGCTACT TGGCCGATGG TCCTGGCAGT GACAGCAGTA GCAGTGGCGG TGGCCGCTCT 
GGCCGGTGCC ACTGCTCCTC CAACGACTCC ATGGTGGACT GCACAGAGGT CAGCAACCAG 
GGGATCTACG GGAGCTGCTC CACTTTCCGC AGCTCGCTGA GTAGCGACTA CGACCCCTAC 
ATCTACCGCA GCAAAAGCCC CTGCCGGAGC GGAGAAAAAA GCAGGGTCCT CCATGGGGAG 
ATGATGCTTC TGGAAGGTTC CTCTGCGCTG CACCTGGTGG CAGACAACAG CCAGTCTGGG 
CCAGCTGCTG CAGCAAGGGA CCAGCTATCT GACTGCAGCC TTGAAATGAA TTACAGCAGT 
AGCAGCAGCA GTAGCCGCTC TTCGCTGGAG CAGAAGGAAC AGGCCTGCAC CCTCTCTGAG 
CGAGGAGGCC CACGGCTGCT CCCTGAGACC ACTGGAGAGA CAGGTCAGAC TGGGAGGGAG 
CCTTGTGACC AAGCGTGTGG CTGCTGCTTG GAAGTACCTG CTGCCCCAAG GGAATTGCCA 
AGTAGGGCAG CTGGTGGCGG CAGCACCATG CTACTTCAGA GCACGCCACT TTGGACAGGG 
GGTGGCTCTT TGCGAGAGCC CCAGACAAGG AGAAGTCAGG GCATGGTCAG CATTAGGACA 
CAGGGGGTCC CTCGTGAAGC CTTGCCCTGT TACTTCTACG AAGAGAAGCG GGTGGCCAGC 
AGTAGCCCCA TCTGGCCAGG CCGTTACACG GAAGACTGTG CGGTGAATGT TCGGTATGTG 
TGTGCAGAAG ACCCCTTGCC GGGCTGCTAT CCGGGCCTGC GTGAGACGGG CCAGTGCATT 
GCCATTATTC CCGAAGTCCA GGATTCCGAG CTGGCCCTGC CAGTAGATTG CTATAGCGGC 
AGCAGCCCTT GGGAGACACC ATGTGGAACA ACAGCCCCCA GCCAGGCCCA GGGGACGCCC 
ATGAAGGCAT TTGGCGAGCC AGGAGAGATA CGGCAAGAAG GGTCATGCCC GCCCATGGTG 
GGCTTCCCGC TGGAGGAGTC CTGCGGGCTC TTTTCCAGCC CTCCGCAAGG TTGTTTGGAG 
GCCGTGGCTG CTGGAGAAGG CCCTCAGACT TTGGTTTCCT ATGGAAACAG CCCTTCCGGC 
TGCTGCAGCC ACAGCCACGT GGTTCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015671264.2
CDS	181..2670
Translation	MHPLGLCNSNDEEDLYEYGWVGVVKLEQPDLEPKPCLTVLGKAKRAVQRGATAVIFDVSENPDAIDQLNQGLEDPLKRPVVYVKGADAVKLMNIVNKQKVARARIQHRPPPQPTEYFDMGIFLAFFVVVSLVCLVLLIKIKLKQRRSQSSMNRLAVQALEKMETWKFKSKLKGPCEGSCGALDTLSNGSASDCAICLEKYLDGEELRVIPCTHRFHRKCVDPWLLQHHTCPHCRHNIIEQKKGNASLVCEETASQLHSRQQQQQQQQQQQRMILPVHFPGRAHRGGHVTAYPTRTSMDPHGNPITVLTVDRHGEAPAFLRSYPPILHLEPHLGTAERQPCTMPHPFQRPKFSGDPFSKPACFSQYETMFQHYYFQGLSYPEPPQSGQFPLHVAAKGLPRAFQPLGSHRLFPPAVHVAPSSQPESGSASGFSCYRGHRSICSGYLADGPGSDSSSSGGGRSGRCHCSSNDSMVDCTEVSNQGIYGSCSTFRSSLSSDYDPYIYRSKSPCRSGEKSRVLHGEMMLLEGSSALHLVADNSQSGPAAAARDQLSDCSLEMNYSSSSSSSRSSLEQKEQACTLSERGGPRLLPETTGETGQTGREPCDQACGCCLEVPAAPRELPSRAAGGGSTMLLQSTPLWTGGGSLREPQTRRSQGMVSIRTQGVPREALPCYFYEEKRVASSSPIWPGRYTEDCAVNVRYVCAEDPLPGCYPGLRETGQCIAIIPEVQDSELALPVDCYSGSSPWETPCGTTAPSQAQGTPMKAFGEPGEIRQEGSCPPMVGFPLEESCGLFSSPPQGCLEAVAAGEGPQTLVSYGNSPSGCCSHSHVVP

Target ORF information:

RefSeq Version	XM_015815778.2
Organism	Protobothrops mucrosquamatus
Definition	Protobothrops mucrosquamatus zinc and ring finger 3 (ZNRF3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015815778.2

ORF Insert Sequence:

ATGCATCCAT TGGGACTCTG CAACAGCAAT GATGAAGAAG ACTTATATGA ATATGGCTGG 
GTTGGAGTTG TGAAGCTGGA GCAGCCTGAC CTGGAACCAA AACCCTGCCT CACTGTTCTG 
GGCAAGGCCA AGCGGGCAGT TCAAAGAGGA GCCACAGCTG TCATTTTTGA TGTGTCCGAA 
AACCCAGATG CCATTGATCA GCTGAATCAG GGCTTGGAGG ACCCATTGAA AAGACCAGTT 
GTTTATGTGA AGGGGGCAGA TGCAGTCAAG CTGATGAACA TTGTTAACAA ACAGAAAGTG 
GCGCGGGCCA GAATCCAGCA CCGTCCACCA CCACAACCCA CCGAATACTT TGACATGGGG 
ATTTTCCTTG CCTTTTTTGT GGTGGTCTCT CTGGTTTGCC TGGTTCTTCT CATCAAGATC 
AAATTGAAGC AGCGTCGCAG CCAGAGCTCT ATGAACAGGC TGGCTGTGCA GGCGCTGGAA 
AAGATGGAGA CGTGGAAGTT CAAATCCAAG CTGAAAGGGC CCTGCGAAGG CAGCTGCGGT 
GCCCTGGACA CGCTCAGCAA CGGCTCTGCC TCGGACTGCG CTATTTGCTT GGAGAAGTAC 
CTGGATGGGG AGGAGCTGCG TGTGATTCCC TGCACCCACC GGTTCCACAG GAAGTGTGTG 
GATCCTTGGC TTCTGCAGCA TCACACTTGC CCCCACTGCC GTCACAACAT CATTGAGCAA 
AAGAAGGGAA ACGCCAGCCT GGTGTGTGAG GAGACGGCCA GCCAGTTACA CAGCCGGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CGCATGATCC TGCCTGTCCA CTTCCCTGGC 
CGAGCACACA GAGGTGGTCA CGTCACCGCC TACCCCACGC GCACCAGCAT GGACCCTCAC 
GGGAACCCCA TCACCGTCTT GACGGTTGAT CGGCATGGAG AGGCCCCGGC CTTCTTACGG 
AGCTACCCGC CCATCCTGCA CCTGGAGCCT CACCTGGGCA CAGCCGAACG CCAGCCCTGT 
ACAATGCCTC ATCCTTTCCA GAGGCCGAAG TTCAGCGGGG ACCCTTTCTC CAAGCCGGCT 
TGCTTCTCCC AGTACGAGAC CATGTTTCAG CACTACTACT TCCAGGGCCT CAGTTACCCT 
GAGCCGCCAC AGAGTGGACA GTTCCCCCTC CACGTGGCTG CCAAGGGCCT CCCCCGTGCC 
TTCCAGCCTC TCGGCAGCCA CAGGCTCTTC CCCCCTGCGG TCCATGTAGC CCCATCCTCC 
CAGCCAGAGA GTGGCAGTGC TTCTGGCTTC AGCTGTTACC GTGGCCACCG CTCGATCTGC 
AGCGGCTACT TGGCCGATGG TCCTGGCAGT GACAGCAGTA GCAGTGGCGG TGGCCGCTCT 
GGCCGGTGCC ACTGCTCCTC CAACGACTCC ATGGTGGACT GCACAGAGGT CAGCAACCAG 
GGGATCTACG GGAGCTGCTC CACTTTCCGC AGCTCGCTGA GTAGCGACTA CGACCCCTAC 
ATCTACCGCA GCAAAAGCCC CTGCCGGAGC GGAGAAAAAA GCAGGGTCCT CCATGGGGAG 
ATGATGCTTC TGGAAGGTTC CTCTGCGCTG CACCTGGTGG CAGACAACAG CCAGTCTGGG 
CCAGCTGCTG CAGCAAGGGA CCAGCTATCT GACTGCAGCC TTGAAATGAA TTACAGCAGT 
AGCAGCAGCA GTAGCCGCTC TTCGCTGGAG CAGAAGGAAC AGGCCTGCAC CCTCTCTGAG 
CGAGGAGGCC CACGGCTGCT CCCTGAGACC ACTGGAGAGA CAGGTCAGAC TGGGAGGGAG 
CCTTGTGACC AAGCGTGTGG CTGCTGCTTG GAAGTACCTG CTGCCCCAAG GGAATTGCCA 
AGTAGGGCAG CTGGTGGCGG CAGCACCATG CTACTTCAGA GCACGCCACT TTGGACAGGG 
GGTGGCTCTT TGCGAGAGCC CCAGACAAGG AGAAGTCAGG GCATGGTCAG CATTAGGACA 
CAGGGGGTCC CTCGTGAAGC CTTGCCCTGT TACTTCTACG AAGAGAAGCG GGTGGCCAGC 
AGTAGCCCCA TCTGGCCAGG CCGTTACACG GAAGACTGTG CGGTGAATGT TCGGTATGTG 
TGTGCAGAAG ACCCCTTGCC GGGCTGCTAT CCGGGCCTGC GTGAGACGGG CCAGTGCATT 
GCCATTATTC CCGAAGTCCA GGATTCCGAG CTGGCCCTGC CAGTAGATTG CTATAGCGGC 
AGCAGCCCTT GGGAGACACC ATGTGGAACA ACAGCCCCCA GCCAGGCCCA GGGGACGCCC 
ATGAAGGCAT TTGGCGAGCC AGGAGAGATA CGGCAAGAAG GGTCATGCCC GCCCATGGTG 
GGCTTCCCGC TGGAGGAGTC CTGCGGGCTC TTTTCCAGCC CTCCGCAAGG TTGTTTGGAG 
GCCGTGGCTG CTGGAGAAGG CCCTCAGACT TTGGTTTCCT ATGGAAACAG CCCTTCCGGC 
TGCTGCAGCC ACAGCCACGT GGTTCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNRF3 ( XM_015815778.1 ) cDNA ORF clone, Protobothrops mucrosquamatus -> XP_015671264.1 Protobothrops mucrosquamatus zinc and ring finger 3 (ZNRF3), mRNA.

Price & Availability↑

CloneID	OPr30060
Clone ID Related Accession (Same CDS sequence)	XM_015815778.1
Accession Version	XM_015815778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-01
Organism	Protobothrops mucrosquamatus
Product	E3 ubiquitin-protein ligase ZNRF3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015386706.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Protobothrops mucrosquamatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGCATCCAT TGGGACTCTG CAACAGCAAT GATGAAGAAG ACTTATATGA ATATGGCTGG 
GTTGGAGTTG TGAAGCTGGA GCAGCCTGAC CTGGAACCAA AACCCTGCCT CACTGTTCTG 
GGCAAGGCCA AGCGGGCAGT TCAAAGAGGA GCCACAGCTG TCATTTTTGA TGTGTCCGAA 
AACCCAGATG CCATTGATCA GCTGAATCAG GGCTTGGAGG ACCCATTGAA AAGACCAGTT 
GTTTATGTGA AGGGGGCAGA TGCAGTCAAG CTGATGAACA TTGTTAACAA ACAGAAAGTG 
GCGCGGGCCA GAATCCAGCA CCGTCCACCA CCACAACCCA CCGAATACTT TGACATGGGG 
ATTTTCCTTG CCTTTTTTGT GGTGGTCTCT CTGGTTTGCC TGGTTCTTCT CATCAAGATC 
AAATTGAAGC AGCGTCGCAG CCAGAGCTCT ATGAACAGGC TGGCTGTGCA GGCGCTGGAA 
AAGATGGAGA CGTGGAAGTT CAAATCCAAG CTGAAAGGGC CCTGCGAAGG CAGCTGCGGT 
GCCCTGGACA CGCTCAGCAA CGGCTCTGCC TCGGACTGCG CTATTTGCTT GGAGAAGTAC 
CTGGATGGGG AGGAGCTGCG TGTGATTCCC TGCACCCACC GGTTCCACAG GAAGTGTGTG 
GATCCTTGGC TTCTGCAGCA TCACACTTGC CCCCACTGCC GTCACAACAT CATTGAGCAA 
AAGAAGGGAA ACGCCAGCCT GGTGTGTGAG GAGACGGCCA GCCAGTTACA CAGCCGGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CGCATGATCC TGCCTGTCCA CTTCCCTGGC 
CGAGCACACA GAGGTGGTCA CGTCACCGCC TACCCCACGC GCACCAGCAT GGACCCTCAC 
GGGAACCCCA TCACCGTCTT GACGGTTGAT CGGCATGGAG AGGCCCCGGC CTTCTTACGG 
AGCTACCCGC CCATCCTGCA CCTGGAGCCT CACCTGGGCA CAGCCGAACG CCAGCCCTGT 
ACAATGCCTC ATCCTTTCCA GAGGCCGAAG TTCAGCGGGG ACCCTTTCTC CAAGCCGGCT 
TGCTTCTCCC AGTACGAGAC CATGTTTCAG CACTACTACT TCCAGGGCCT CAGTTACCCT 
GAGCCGCCAC AGAGTGGACA GTTCCCCCTC CACGTGGCTG CCAAGGGCCT CCCCCGTGCC 
TTCCAGCCTC TCGGCAGCCA CAGGCTCTTC CCCCCTGCGG TCCATGTAGC CCCATCCTCC 
CAGCCAGAGA GTGGCAGTGC TTCTGGCTTC AGCTGTTACC GTGGCCACCG CTCGATCTGC 
AGCGGCTACT TGGCCGATGG TCCTGGCAGT GACAGCAGTA GCAGTGGCGG TGGCCGCTCT 
GGCCGGTGCC ACTGCTCCTC CAACGACTCC ATGGTGGACT GCACAGAGGT CAGCAACCAG 
GGGATCTACG GGAGCTGCTC CACTTTCCGC AGCTCGCTGA GTAGCGACTA CGACCCCTAC 
ATCTACCGCA GCAAAAGCCC CTGCCGGAGC GGAGAAAAAA GCAGGGTCCT CCATGGGGAG 
ATGATGCTTC TGGAAGGTTC CTCTGCGCTG CACCTGGTGG CAGACAACAG CCAGTCTGGG 
CCAGCTGCTG CAGCAAGGGA CCAGCTATCT GACTGCAGCC TTGAAATGAA TTACAGCAGT 
AGCAGCAGCA GTAGCCGCTC TTCGCTGGAG CAGAAGGAAC AGGCCTGCAC CCTCTCTGAG 
CGAGGAGGCC CACGGCTGCT CCCTGAGACC ACTGGAGAGA CAGGTCAGAC TGGGAGGGAG 
CCTTGTGACC AAGCGTGTGG CTGCTGCTTG GAAGTACCTG CTGCCCCAAG GGAATTGCCA 
AGTAGGGCAG CTGGTGGCGG CAGCACCATG CTACTTCAGA GCACGCCACT TTGGACAGGG 
GGTGGCTCTT TGCGAGAGCC CCAGACAAGG AGAAGTCAGG GCATGGTCAG CATTAGGACA 
CAGGGGGTCC CTCGTGAAGC CTTGCCCTGT TACTTCTACG AAGAGAAGCG GGTGGCCAGC 
AGTAGCCCCA TCTGGCCAGG CCGTTACACG GAAGACTGTG CGGTGAATGT TCGGTATGTG 
TGTGCAGAAG ACCCCTTGCC GGGCTGCTAT CCGGGCCTGC GTGAGACGGG CCAGTGCATT 
GCCATTATTC CCGAAGTCCA GGATTCCGAG CTGGCCCTGC CAGTAGATTG CTATAGCGGC 
AGCAGCCCTT GGGAGACACC ATGTGGAACA ACAGCCCCCA GCCAGGCCCA GGGGACGCCC 
ATGAAGGCAT TTGGCGAGCC AGGAGAGATA CGGCAAGAAG GGTCATGCCC GCCCATGGTG 
GGCTTCCCGC TGGAGGAGTC CTGCGGGCTC TTTTCCAGCC CTCCGCAAGG TTGTTTGGAG 
GCCGTGGCTG CTGGAGAAGG CCCTCAGACT TTGGGTAAGT CAGCAGCGCC ATCTTGGCTG 
CAGGCGAGGT GGGTGGAGCA GGGAGGGAAG GGGGATGCCT TTGTTCAGCC AAGCTGGCCA 
AATGCCTCCC AAGAGCTGCA GGGGGCCGAT CCAGGCTTGT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015671264.1
CDS	10..2574
Translation	MHPLGLCNSNDEEDLYEYGWVGVVKLEQPDLEPKPCLTVLGKAKRAVQRGATAVIFDVSENPDAIDQLNQGLEDPLKRPVVYVKGADAVKLMNIVNKQKVARARIQHRPPPQPTEYFDMGIFLAFFVVVSLVCLVLLIKIKLKQRRSQSSMNRLAVQALEKMETWKFKSKLKGPCEGSCGALDTLSNGSASDCAICLEKYLDGEELRVIPCTHRFHRKCVDPWLLQHHTCPHCRHNIIEQKKGNASLVCEETASQLHSRQQQQQQQQQQQRMILPVHFPGRAHRGGHVTAYPTRTSMDPHGNPITVLTVDRHGEAPAFLRSYPPILHLEPHLGTAERQPCTMPHPFQRPKFSGDPFSKPACFSQYETMFQHYYFQGLSYPEPPQSGQFPLHVAAKGLPRAFQPLGSHRLFPPAVHVAPSSQPESGSASGFSCYRGHRSICSGYLADGPGSDSSSSGGGRSGRCHCSSNDSMVDCTEVSNQGIYGSCSTFRSSLSSDYDPYIYRSKSPCRSGEKSRVLHGEMMLLEGSSALHLVADNSQSGPAAAARDQLSDCSLEMNYSSSSSSSRSSLEQKEQACTLSERGGPRLLPETTGETGQTGREPCDQACGCCLEVPAAPRELPSRAAGGGSTMLLQSTPLWTGGGSLREPQTRRSQGMVSIRTQGVPREALPCYFYEEKRVASSSPIWPGRYTEDCAVNVRYVCAEDPLPGCYPGLRETGQCIAIIPEVQDSELALPVDCYSGSSPWETPCGTTAPSQAQGTPMKAFGEPGEIRQEGSCPPMVGFPLEESCGLFSSPPQGCLEAVAAGEGPQTLGKSAAPSWLQARWVEQGGKGDAFVQPSWPNASQELQGADPGLF

Target ORF information:

RefSeq Version	XM_015815778.1
Organism	Protobothrops mucrosquamatus
Definition	Protobothrops mucrosquamatus zinc and ring finger 3 (ZNRF3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015815778.1

ORF Insert Sequence:

ATGCATCCAT TGGGACTCTG CAACAGCAAT GATGAAGAAG ACTTATATGA ATATGGCTGG 
GTTGGAGTTG TGAAGCTGGA GCAGCCTGAC CTGGAACCAA AACCCTGCCT CACTGTTCTG 
GGCAAGGCCA AGCGGGCAGT TCAAAGAGGA GCCACAGCTG TCATTTTTGA TGTGTCCGAA 
AACCCAGATG CCATTGATCA GCTGAATCAG GGCTTGGAGG ACCCATTGAA AAGACCAGTT 
GTTTATGTGA AGGGGGCAGA TGCAGTCAAG CTGATGAACA TTGTTAACAA ACAGAAAGTG 
GCGCGGGCCA GAATCCAGCA CCGTCCACCA CCACAACCCA CCGAATACTT TGACATGGGG 
ATTTTCCTTG CCTTTTTTGT GGTGGTCTCT CTGGTTTGCC TGGTTCTTCT CATCAAGATC 
AAATTGAAGC AGCGTCGCAG CCAGAGCTCT ATGAACAGGC TGGCTGTGCA GGCGCTGGAA 
AAGATGGAGA CGTGGAAGTT CAAATCCAAG CTGAAAGGGC CCTGCGAAGG CAGCTGCGGT 
GCCCTGGACA CGCTCAGCAA CGGCTCTGCC TCGGACTGCG CTATTTGCTT GGAGAAGTAC 
CTGGATGGGG AGGAGCTGCG TGTGATTCCC TGCACCCACC GGTTCCACAG GAAGTGTGTG 
GATCCTTGGC TTCTGCAGCA TCACACTTGC CCCCACTGCC GTCACAACAT CATTGAGCAA 
AAGAAGGGAA ACGCCAGCCT GGTGTGTGAG GAGACGGCCA GCCAGTTACA CAGCCGGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CGCATGATCC TGCCTGTCCA CTTCCCTGGC 
CGAGCACACA GAGGTGGTCA CGTCACCGCC TACCCCACGC GCACCAGCAT GGACCCTCAC 
GGGAACCCCA TCACCGTCTT GACGGTTGAT CGGCATGGAG AGGCCCCGGC CTTCTTACGG 
AGCTACCCGC CCATCCTGCA CCTGGAGCCT CACCTGGGCA CAGCCGAACG CCAGCCCTGT 
ACAATGCCTC ATCCTTTCCA GAGGCCGAAG TTCAGCGGGG ACCCTTTCTC CAAGCCGGCT 
TGCTTCTCCC AGTACGAGAC CATGTTTCAG CACTACTACT TCCAGGGCCT CAGTTACCCT 
GAGCCGCCAC AGAGTGGACA GTTCCCCCTC CACGTGGCTG CCAAGGGCCT CCCCCGTGCC 
TTCCAGCCTC TCGGCAGCCA CAGGCTCTTC CCCCCTGCGG TCCATGTAGC CCCATCCTCC 
CAGCCAGAGA GTGGCAGTGC TTCTGGCTTC AGCTGTTACC GTGGCCACCG CTCGATCTGC 
AGCGGCTACT TGGCCGATGG TCCTGGCAGT GACAGCAGTA GCAGTGGCGG TGGCCGCTCT 
GGCCGGTGCC ACTGCTCCTC CAACGACTCC ATGGTGGACT GCACAGAGGT CAGCAACCAG 
GGGATCTACG GGAGCTGCTC CACTTTCCGC AGCTCGCTGA GTAGCGACTA CGACCCCTAC 
ATCTACCGCA GCAAAAGCCC CTGCCGGAGC GGAGAAAAAA GCAGGGTCCT CCATGGGGAG 
ATGATGCTTC TGGAAGGTTC CTCTGCGCTG CACCTGGTGG CAGACAACAG CCAGTCTGGG 
CCAGCTGCTG CAGCAAGGGA CCAGCTATCT GACTGCAGCC TTGAAATGAA TTACAGCAGT 
AGCAGCAGCA GTAGCCGCTC TTCGCTGGAG CAGAAGGAAC AGGCCTGCAC CCTCTCTGAG 
CGAGGAGGCC CACGGCTGCT CCCTGAGACC ACTGGAGAGA CAGGTCAGAC TGGGAGGGAG 
CCTTGTGACC AAGCGTGTGG CTGCTGCTTG GAAGTACCTG CTGCCCCAAG GGAATTGCCA 
AGTAGGGCAG CTGGTGGCGG CAGCACCATG CTACTTCAGA GCACGCCACT TTGGACAGGG 
GGTGGCTCTT TGCGAGAGCC CCAGACAAGG AGAAGTCAGG GCATGGTCAG CATTAGGACA 
CAGGGGGTCC CTCGTGAAGC CTTGCCCTGT TACTTCTACG AAGAGAAGCG GGTGGCCAGC 
AGTAGCCCCA TCTGGCCAGG CCGTTACACG GAAGACTGTG CGGTGAATGT TCGGTATGTG 
TGTGCAGAAG ACCCCTTGCC GGGCTGCTAT CCGGGCCTGC GTGAGACGGG CCAGTGCATT 
GCCATTATTC CCGAAGTCCA GGATTCCGAG CTGGCCCTGC CAGTAGATTG CTATAGCGGC 
AGCAGCCCTT GGGAGACACC ATGTGGAACA ACAGCCCCCA GCCAGGCCCA GGGGACGCCC 
ATGAAGGCAT TTGGCGAGCC AGGAGAGATA CGGCAAGAAG GGTCATGCCC GCCCATGGTG 
GGCTTCCCGC TGGAGGAGTC CTGCGGGCTC TTTTCCAGCC CTCCGCAAGG TTGTTTGGAG 
GCCGTGGCTG CTGGAGAAGG CCCTCAGACT TTGGGTAAGT CAGCAGCGCC ATCTTGGCTG 
CAGGCGAGGT GGGTGGAGCA GGGAGGGAAG GGGGATGCCT TTGTTCAGCC AAGCTGGCCA 
AATGCCTCCC AAGAGCTGCA GGGGGCCGAT CCAGGCTTGT TTTAG