NPEPPS cDNA ORF clone, Protobothrops mucrosquamatus

Gene
Clones
gRNAs

Summary

Gene Symbol	NPEPPS
Entrez Gene ID	107282639
Full Name	aminopeptidase puromycin sensitive
Gene Type	protein-coding
Organism	Protobothrops mucrosquamatus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015810449.1	XP_015665935.1	puromycin-sensitive aminopeptidase

Pathways from BioSystems
Gene Ontology

The following NPEPPS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPEPPS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPr25601	XM_015810449.1 Latest version!	Protobothrops mucrosquamatus aminopeptidase puromycin sensitive (NPEPPS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPEPPS ( XM_015810449.1 ) cDNA ORF clone, Protobothrops mucrosquamatus -> XP_015665935.1 Protobothrops mucrosquamatus aminopeptidase puromycin sensitive (NPEPPS), mRNA.

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CloneID	OPr25601
Clone ID Related Accession (Same CDS sequence)	XM_015810449.1
Accession Version	XM_015810449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-22
Organism	Protobothrops mucrosquamatus
Product	puromycin-sensitive aminopeptidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015386397.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Protobothrops mucrosquamatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGAAA AGCGGCCCTT CGAGCGGCTC CCGACCGAAG TGCGCCCCAT CAATTATGGG 
TTGTGTCTCA AGCCCGACCT TATCGACTTC ACCTTCGAGG GAAAACTCGA GGCCGCCGTA 
GAGGTGAAGC AGGCGACCAA TCAAATAGTG ATGAATTGTG CTGACATTGA TATTATTACA 
GCTTCATATG CACCAGAAGG AGATGAAGAA ATCCATGCCA CAGGGTTCAA CTATCAGAAT 
GAAGACGAGA AAGTGACTCT TTCTTTTCCC AGCACACTTC AGAAAGGGAT GGGAACATTA 
AAGATAGATT TTGTTGGAGA ATTGAATGAC AAAATGAAAG GATTCTATCG AAGTAAATAC 
ATCACCCCTT CCGGAGATAC TCGGTTTGCT GCTGTTACCC AATTTGAGGC CACTGATGCT 
CGCAGGGCCT TTCCTTGTTG GGATGAGCCA GCTATCAAGG CAACTTTTGA TATTTCATTG 
GTGGTTCCTA AAGACAGAGT AGCTCTGTCA AATATGAATA TCACTGATCG GAGGCCCTAT 
CCTGATGATG AAAATCTGGT AGAAGTGAAA TTTGCTTGTA CTCCTGTGAT GTCAACATAT 
CTTGTTGCTT TTGTGGTCGG AGAATATGAC TTTGTTGAGA CCAGGACCAC TGATGGTGTA 
TTAATCCGTG TTTACACTCC TGTTGGCAAA GCAGAACAAG GGAAGTTTGC ATTAGAGGTT 
GCTGCTAAAA CTTTGCCTTT TTATAAGGAC TACTTCAATG TTCCTTACCC TCTTCCTAAA 
ATTGATCTTA TAGCTATTGC AGACTTTGCA GCAGGTGCCA TGGAGAACTG GGGCCTTGTT 
ACTTATAGGG AAACTGCACT GCTAATTGAT CCCAAAAACT CTTGTTCTTC CTCTCGTCAA 
TGGGTAGCCC TTGTTGTGGG CCATGAACTT GCTCACCAGT GGTTTGGAAA CCTCGTGACT 
ATGGAATGGT GGACTCACCT GTGGCTCAAT GAGGGCTTTG CATCCTGGAT TGAGTACCTT 
TGTGTAGACC ATTGCTTTCC AGAATATGAT ATCTGGACTC AGTTTGTATC TGCTGATTAC 
ACAAGAGCTC AGGAGCTTGA TGCATTAGAC AACAGTCATC CTATTGAAGT CAATGTAGGC 
CATCCATCTG AAGTGGATGA AATATTTGAT GCAATTTCTT ACAGCAAGGG AGCATCAGTA 
ATTCGAATGC TACATGATTA TATAGGTGAT GAGGATTTCC GAAAAGGAAT GCACCTGTAT 
CTTACCAAGT TTCAGCATAA GAATGCTTCT ACAGAAGATC TTTGGGAAAG CCTAGAAGAC 
GCTAGTGGTA AACCTATTGC TGCTGTGATG AATACATGGA CCAAGCAAAT GGGTTTTCCA 
CTGGTATACG TAGAAGGGGA GCAGCAAGAA GATAACAAAG TGCTGAAGCT AGTCCAGAAG 
AAATTCTGTG CCAGTGGACC ATATAGTGGG GAAGATTGCC CTTTGTGGAT GATTCCCATT 
ACCATTTGTA CAAGTGATGA TCCAATCCAT GCCAAAATGC AAGTATTAAT GGACAAGCCT 
GAGTTAACTT TAGTTTTGAA AGATGTGAAA CCAGAGCAAT GGATTAAGCT AAATCTGGGA 
ACTGTGGGAT TTTATCGGAC ACAATACAGT CCTGACATGC TGGAGAGTTT ACTTCCAGCT 
ATCCGTGATC TCTCCTTACT CCCTGTGGAC AGACTAGGGC TGCAGAATGA TCTCTTTTCC 
TTGGCCAGAG CTGGAATTAT TAGCACTGTA GAGGTTCTAA AAATCATGGA AGCTTTTGTG 
AATGAGCCCA ACTATACTGT CTGGAGCGAC CTAAGCTGTA ACCTGGGGAT TCTCTCAACT 
CTCTTGTCCC ACACAGACTT CTACGAAGAA ATTCAGGCAT TCATCCGTGA TATCTTTTCA 
CCAATAGGAG AAAAACTGGG CTGGGACCCC AGGCCTGGTG AGGGTCATCT AGATGCACTT 
CTGAGAGGTT TAGTTTTGGG CAAGCTTGGA AAAGCTGGAC ACAAGGCAAC TTTGGAAGAA 
GCTCGGCGAC GGTTTAAAGA TCATGTGGAA GGGAAGCACA TCCTGTCTGC TGATCTAAGG 
AGTTCTGTAT ATGTAACTGT TTTGAAACAT GGTGATAGTA TAACCTTAGA TACTATGCTA 
AAGCTCCACA AGCAAGCTGA TATGCAGGAA GAGAAGAACA GAATAGAACG TGTACTTGGT 
GCAATATCGC AACCAGAGTT AATACAGAAA GTTCTTACTT TTGCACTTTC AGAAGAGGTA 
CGTCCCCAAG ACACAGTATC TGTCATTGGT GGAGTTGCAG GAGGCAGCAA GCAGGGCAGA 
AAGGCTGCCT GGAAGTTCGT CAGAGACAAT TGGGAGGAAC TATACAATCG CTACCAGGGG 
GGATTCTTAA TATCCAGACT AATAAAGCTA TCAGTGGATG GATTTGCAAT TGACAAAATG 
GCTTCAGAAG TTAAGGCTTT TTTTGAGAGT CATCCAGCTC CATCAGCTGA GCGTACCATA 
CAGCAGTGCT GTGAAAACAT CCTGTTGAAT GCTGCATGGC TGAAGCGTGA TGCTGAAAAT 
GTCCATCAGT ACCTCCTGCA ACGCAAAGTC TCACCAACTG CTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015665935.1
CDS	23..2650
Translation	MPEKRPFERLPTEVRPINYGLCLKPDLIDFTFEGKLEAAVEVKQATNQIVMNCADIDIITASYAPEGDEEIHATGFNYQNEDEKVTLSFPSTLQKGMGTLKIDFVGELNDKMKGFYRSKYITPSGDTRFAAVTQFEATDARRAFPCWDEPAIKATFDISLVVPKDRVALSNMNITDRRPYPDDENLVEVKFACTPVMSTYLVAFVVGEYDFVETRTTDGVLIRVYTPVGKAEQGKFALEVAAKTLPFYKDYFNVPYPLPKIDLIAIADFAAGAMENWGLVTYRETALLIDPKNSCSSSRQWVALVVGHELAHQWFGNLVTMEWWTHLWLNEGFASWIEYLCVDHCFPEYDIWTQFVSADYTRAQELDALDNSHPIEVNVGHPSEVDEIFDAISYSKGASVIRMLHDYIGDEDFRKGMHLYLTKFQHKNASTEDLWESLEDASGKPIAAVMNTWTKQMGFPLVYVEGEQQEDNKVLKLVQKKFCASGPYSGEDCPLWMIPITICTSDDPIHAKMQVLMDKPELTLVLKDVKPEQWIKLNLGTVGFYRTQYSPDMLESLLPAIRDLSLLPVDRLGLQNDLFSLARAGIISTVEVLKIMEAFVNEPNYTVWSDLSCNLGILSTLLSHTDFYEEIQAFIRDIFSPIGEKLGWDPRPGEGHLDALLRGLVLGKLGKAGHKATLEEARRRFKDHVEGKHILSADLRSSVYVTVLKHGDSITLDTMLKLHKQADMQEEKNRIERVLGAISQPELIQKVLTFALSEEVRPQDTVSVIGGVAGGSKQGRKAAWKFVRDNWEELYNRYQGGFLISRLIKLSVDGFAIDKMASEVKAFFESHPAPSAERTIQQCCENILLNAAWLKRDAENVHQYLLQRKVSPTAV

Target ORF information:

RefSeq Version	XM_015810449.1
Organism	Protobothrops mucrosquamatus
Definition	Protobothrops mucrosquamatus aminopeptidase puromycin sensitive (NPEPPS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015810449.1

ORF Insert Sequence:

ATGCCGGAAA AGCGGCCCTT CGAGCGGCTC CCGACCGAAG TGCGCCCCAT CAATTATGGG 
TTGTGTCTCA AGCCCGACCT TATCGACTTC ACCTTCGAGG GAAAACTCGA GGCCGCCGTA 
GAGGTGAAGC AGGCGACCAA TCAAATAGTG ATGAATTGTG CTGACATTGA TATTATTACA 
GCTTCATATG CACCAGAAGG AGATGAAGAA ATCCATGCCA CAGGGTTCAA CTATCAGAAT 
GAAGACGAGA AAGTGACTCT TTCTTTTCCC AGCACACTTC AGAAAGGGAT GGGAACATTA 
AAGATAGATT TTGTTGGAGA ATTGAATGAC AAAATGAAAG GATTCTATCG AAGTAAATAC 
ATCACCCCTT CCGGAGATAC TCGGTTTGCT GCTGTTACCC AATTTGAGGC CACTGATGCT 
CGCAGGGCCT TTCCTTGTTG GGATGAGCCA GCTATCAAGG CAACTTTTGA TATTTCATTG 
GTGGTTCCTA AAGACAGAGT AGCTCTGTCA AATATGAATA TCACTGATCG GAGGCCCTAT 
CCTGATGATG AAAATCTGGT AGAAGTGAAA TTTGCTTGTA CTCCTGTGAT GTCAACATAT 
CTTGTTGCTT TTGTGGTCGG AGAATATGAC TTTGTTGAGA CCAGGACCAC TGATGGTGTA 
TTAATCCGTG TTTACACTCC TGTTGGCAAA GCAGAACAAG GGAAGTTTGC ATTAGAGGTT 
GCTGCTAAAA CTTTGCCTTT TTATAAGGAC TACTTCAATG TTCCTTACCC TCTTCCTAAA 
ATTGATCTTA TAGCTATTGC AGACTTTGCA GCAGGTGCCA TGGAGAACTG GGGCCTTGTT 
ACTTATAGGG AAACTGCACT GCTAATTGAT CCCAAAAACT CTTGTTCTTC CTCTCGTCAA 
TGGGTAGCCC TTGTTGTGGG CCATGAACTT GCTCACCAGT GGTTTGGAAA CCTCGTGACT 
ATGGAATGGT GGACTCACCT GTGGCTCAAT GAGGGCTTTG CATCCTGGAT TGAGTACCTT 
TGTGTAGACC ATTGCTTTCC AGAATATGAT ATCTGGACTC AGTTTGTATC TGCTGATTAC 
ACAAGAGCTC AGGAGCTTGA TGCATTAGAC AACAGTCATC CTATTGAAGT CAATGTAGGC 
CATCCATCTG AAGTGGATGA AATATTTGAT GCAATTTCTT ACAGCAAGGG AGCATCAGTA 
ATTCGAATGC TACATGATTA TATAGGTGAT GAGGATTTCC GAAAAGGAAT GCACCTGTAT 
CTTACCAAGT TTCAGCATAA GAATGCTTCT ACAGAAGATC TTTGGGAAAG CCTAGAAGAC 
GCTAGTGGTA AACCTATTGC TGCTGTGATG AATACATGGA CCAAGCAAAT GGGTTTTCCA 
CTGGTATACG TAGAAGGGGA GCAGCAAGAA GATAACAAAG TGCTGAAGCT AGTCCAGAAG 
AAATTCTGTG CCAGTGGACC ATATAGTGGG GAAGATTGCC CTTTGTGGAT GATTCCCATT 
ACCATTTGTA CAAGTGATGA TCCAATCCAT GCCAAAATGC AAGTATTAAT GGACAAGCCT 
GAGTTAACTT TAGTTTTGAA AGATGTGAAA CCAGAGCAAT GGATTAAGCT AAATCTGGGA 
ACTGTGGGAT TTTATCGGAC ACAATACAGT CCTGACATGC TGGAGAGTTT ACTTCCAGCT 
ATCCGTGATC TCTCCTTACT CCCTGTGGAC AGACTAGGGC TGCAGAATGA TCTCTTTTCC 
TTGGCCAGAG CTGGAATTAT TAGCACTGTA GAGGTTCTAA AAATCATGGA AGCTTTTGTG 
AATGAGCCCA ACTATACTGT CTGGAGCGAC CTAAGCTGTA ACCTGGGGAT TCTCTCAACT 
CTCTTGTCCC ACACAGACTT CTACGAAGAA ATTCAGGCAT TCATCCGTGA TATCTTTTCA 
CCAATAGGAG AAAAACTGGG CTGGGACCCC AGGCCTGGTG AGGGTCATCT AGATGCACTT 
CTGAGAGGTT TAGTTTTGGG CAAGCTTGGA AAAGCTGGAC ACAAGGCAAC TTTGGAAGAA 
GCTCGGCGAC GGTTTAAAGA TCATGTGGAA GGGAAGCACA TCCTGTCTGC TGATCTAAGG 
AGTTCTGTAT ATGTAACTGT TTTGAAACAT GGTGATAGTA TAACCTTAGA TACTATGCTA 
AAGCTCCACA AGCAAGCTGA TATGCAGGAA GAGAAGAACA GAATAGAACG TGTACTTGGT 
GCAATATCGC AACCAGAGTT AATACAGAAA GTTCTTACTT TTGCACTTTC AGAAGAGGTA 
CGTCCCCAAG ACACAGTATC TGTCATTGGT GGAGTTGCAG GAGGCAGCAA GCAGGGCAGA 
AAGGCTGCCT GGAAGTTCGT CAGAGACAAT TGGGAGGAAC TATACAATCG CTACCAGGGG 
GGATTCTTAA TATCCAGACT AATAAAGCTA TCAGTGGATG GATTTGCAAT TGACAAAATG 
GCTTCAGAAG TTAAGGCTTT TTTTGAGAGT CATCCAGCTC CATCAGCTGA GCGTACCATA 
CAGCAGTGCT GTGAAAACAT CCTGTTGAAT GCTGCATGGC TGAAGCGTGA TGCTGAAAAT 
GTCCATCAGT ACCTCCTGCA ACGCAAAGTC TCACCAACTG CTGTGTGA