MAP3K12 cDNA ORF clone, Propithecus coquereli(Coquerel's sifaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP3K12
Entrez Gene ID	105817525
Full Name	mitogen-activated protein kinase kinase kinase 12
Gene Type	protein-coding
Organism	Propithecus coquereli(Coquerel's sifaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012653246.1	XP_012508700.1	mitogen-activated protein kinase kinase kinase 12 isoform X2
XM_012653238.1	XP_012508692.1	mitogen-activated protein kinase kinase kinase 12 isoform X1

Pathways from BioSystems
Gene Ontology

The following MAP3K12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPr96686	XM_012653238.1 Latest version!	Propithecus coquereli mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPr96687	XM_012653246.1 Latest version!	Propithecus coquereli mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP3K12 ( XM_012653238.1 ) cDNA ORF clone, Propithecus coquereli(Coquerel's sifaka) -> XP_012508692.1 Propithecus coquereli mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPr96686
Clone ID Related Accession (Same CDS sequence)	XM_012653238.1
Accession Version	XM_012653238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-31
Organism	Propithecus coquereli(Coquerel's sifaka)
Product	mitogen-activated protein kinase kinase kinase 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012135081.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Propithecus coquereli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGCC TCCATGAGAC CCGAACACCC TCCCCTTCCT TTGGGGGCTT CGTGTCTACC 
CTAAGCGAGG CATCCATGCG CAAGCTGGAC CCAGACACTT CCGACTGCAC TCCCGAAAAG 
GACCTGACGC CTACCCAGTG TGTACTTCGA GATGTAGTAC CCCTTGGTGG GCAGGCTGGG 
GGTGGGCCCA GCCCCTCCCC AGGTGGAGAG CCACCCCCTG AGCCTTTTGC CAACAGTGTC 
CTGCAGCTAC ATGAGCAGGA CACAGGGGGC CCAGGGGGAG CCCCTGGGTC ACCTGAGAGT 
CGGGCGTCCA GAGTTCGAGC AGATGAGGTG CGGCTACAGT GCCAGAGTGG CAGTGGCTTT 
CTTGAAGGCC TCTTTGGCTG CCTGCGCCCT GTCTGGACCA TGATTGGCAA AGCCTATTCC 
ACCGAGCACA AGCAGCAGCA GGAAGACCTT TGGGAGGTCC CCTTTGAGGA AATCCTGGAC 
CTGCAGTGGG TGGGCTCAGG GGCCCAGGGT GCTGTCTTCC TGGGGCGCTT CCATGGGGAG 
GAGGTGGCTG TGAAGAAGGT ACGAGACCTT AAAGAGACCG ACATCAAGCA CCTGCGCAAG 
CTGAAGCACC CCAACATCAT CACTTTCAAG GGTGTGTGCA CCCAGGCTCC TTGCTACTGC 
ATCCTCATGG AGTTCTGCGC CCAGGGTCAG CTATATGAGG TACTACGGGC TGGCCGCCCC 
GTCACCCCCT CCTTGCTGGT TGACTGGTCC ATGGGCATCG CTGGTGGCAT GAACTACCTG 
CACCTGCACA AGATTATCCA CAGAGACCTC AAGTCCCCTA ACATGCTAAT CACATATGAC 
GACGTGGTGA AGATCTCAGA TTTTGGCACT TCCAAGGAGC TGAGTGACAA GAGCACCAAG 
ATGTCCTTTG CAGGGACAGT AGCCTGGATG GCCCCTGAGG TGATCCGCAA TGAGCCTGTG 
TCTGAGAAGG TCGACATCTG GTCCTTTGGT GTAGTGTTGT GGGAACTGCT GACTGGAGAG 
ATCCCCTACA AAGACGTAGA TTCCTCAGCC ATCATCTGGG GTGTAGGAAG CAACAGTCTC 
CATCTGCCTG TGCCCTCCAG CTGCCCAGAT GGCTTCAAAA TCCTGCTTCG CCAGTGCTGG 
AATAGCAAAC CACGAAATCG TCCATCATTC CGACAGATCC TGTTGCATCT GGACATTGCC 
TCAGCTGATG TACTCTCCAC ACCCCAGGAG ACTTACTTTA AGTCCCAGGC AGAGTGGCGG 
GAAGAAGTAA AGCTGCACTT TGAAAAGATT AAGTCAGAAG GGACCTGTCT GCACCGCCTA 
GAAGAGGAGC TGGTGATGCG GAGGAGGGAG GAGCTCAGAC ATGCATTGGA TATCAGGGAG 
CACTACGAAC GCAAGCTGGA GAGAGCCAAC AACCTGTACA TGGAGTTAAA TGCCCTCATG 
TTGCAGCTGG AACTCAAGGA ACGAGAGTTG CTCAGGCGGG AGCAAGCTTT AGAGCGGAGG 
TGCCCAGGCC TACTTAAGTC ACACCCTTCC CGGGGCCTTC TGCATGGGAA CACAATGGAG 
AAGCTCATTA AGAAGAGGAA CATGCCACAG AAGCTCTCAC CCCATAGCAA AAGGCCAGAT 
ATCCTCAAGA CAGAGTCTTT GCTACCTAAA CTAGATGCAG CCCTAAGTGG GGTGGGGCTT 
CCTGGGTGTC CTAAGGGCCC CCCCTCACCA GGACGGAGTC GCCGTGGCAA GACCCGTCAC 
CGCAAGGCCA GCGCCAAGGG CAGCTGTGGG GACCTGCCTG GGCTTCGTGC AGCTATGCCA 
CCCCATGAAC CTGGGGGACC AGGAAGCCCA GGGGGAGTGG GACCCTCAGC CTGGGAGGCC 
TGTCCCCCCA CCCTCTGTGG GCTCCATCAT GACCTTCTGC TCCGCAAGAT GTCTTCGTCA 
TCCCCAGACC TGCTGTCAGC AGCACTGGGG TCCCGGAGCC GGGGAGCCAC AGGAGGAGCT 
GGGGATCCTG GCTCACCACC TCCAGCCCGG GGTGACACCC CCCCAAGTGA GGGCTCAGCC 
CCTGGCTCCA CCAGCCCAGA TTCACCTGGG GGAGCCAAAG GGGAGCCACC TCCACCAGTA 
GGACCTGGTG AAGGTGTGGG GCTGCTGGGA ACTGGAAGGG AAGGGACTTC GGGCCGGGGA 
GGAAGCCGGG CTGGGTCCCA GCACTTGACC CCAGCTGCAC TCCTGTACAG GGCTGCTGTC 
ACCCGCAGTC AGAAACGAGG CATCTCTTCA GAAGAGGAGG AAGGAGAGGT GGACAGTGAA 
GTAGAACTGA CTTCAAGCCA GAGGTGGCCT CAAGGCCTGA ACATGCGCCA GTCACTATCT 
ACCTTCAGCT CAGAGAATCC ATCAGATGGG GAGGAAGGTA CAGCTAGTGA GCCTTCCCCC 
AGCGGCACCC CTGAAGTTGG CAGTACCAAC ACTGATGAGC GGCCCGATGA ACGGTCTGAT 
GACATGTGCT CCCAGGGCTC AGAAATCCCA CTGGACCCAC CTCCTTTGGA GGTGGTCCCT 
GACCCTGTAC CCAGCACCGT GCCCATGCCA CACCAGGACC TACTCAGAGG GGAGCAGGGC 
CCTCCCAATA CTGAGGACTC AGACTGTGAC AGCACTGAAT TGGACAACTC CAACAGCATT 
GATGCCTTGC GGCCCCCAGC CTCCCTCCCT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012508692.1
CDS	273..2948
Translation	MACLHETRTPSPSFGGFVSTLSEASMRKLDPDTSDCTPEKDLTPTQCVLRDVVPLGGQAGGGPSPSPGGEPPPEPFANSVLQLHEQDTGGPGGAPGSPESRASRVRADEVRLQCQSGSGFLEGLFGCLRPVWTMIGKAYSTEHKQQQEDLWEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKETDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASADVLSTPQETYFKSQAEWREEVKLHFEKIKSEGTCLHRLEEELVMRRREELRHALDIREHYERKLERANNLYMELNALMLQLELKERELLRREQALERRCPGLLKSHPSRGLLHGNTMEKLIKKRNMPQKLSPHSKRPDILKTESLLPKLDAALSGVGLPGCPKGPPSPGRSRRGKTRHRKASAKGSCGDLPGLRAAMPPHEPGGPGSPGGVGPSAWEACPPTLCGLHHDLLLRKMSSSSPDLLSAALGSRSRGATGGAGDPGSPPPARGDTPPSEGSAPGSTSPDSPGGAKGEPPPPVGPGEGVGLLGTGREGTSGRGGSRAGSQHLTPAALLYRAAVTRSQKRGISSEEEEGEVDSEVELTSSQRWPQGLNMRQSLSTFSSENPSDGEEGTASEPSPSGTPEVGSTNTDERPDERSDDMCSQGSEIPLDPPPLEVVPDPVPSTVPMPHQDLLRGEQGPPNTEDSDCDSTELDNSNSIDALRPPASLPP

Target ORF information:

RefSeq Version	XM_012653238.1
Organism	Propithecus coquereli(Coquerel's sifaka)
Definition	Propithecus coquereli mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012653238.1

ORF Insert Sequence:

ATGGCCTGCC TCCATGAGAC CCGAACACCC TCCCCTTCCT TTGGGGGCTT CGTGTCTACC 
CTAAGCGAGG CATCCATGCG CAAGCTGGAC CCAGACACTT CCGACTGCAC TCCCGAAAAG 
GACCTGACGC CTACCCAGTG TGTACTTCGA GATGTAGTAC CCCTTGGTGG GCAGGCTGGG 
GGTGGGCCCA GCCCCTCCCC AGGTGGAGAG CCACCCCCTG AGCCTTTTGC CAACAGTGTC 
CTGCAGCTAC ATGAGCAGGA CACAGGGGGC CCAGGGGGAG CCCCTGGGTC ACCTGAGAGT 
CGGGCGTCCA GAGTTCGAGC AGATGAGGTG CGGCTACAGT GCCAGAGTGG CAGTGGCTTT 
CTTGAAGGCC TCTTTGGCTG CCTGCGCCCT GTCTGGACCA TGATTGGCAA AGCCTATTCC 
ACCGAGCACA AGCAGCAGCA GGAAGACCTT TGGGAGGTCC CCTTTGAGGA AATCCTGGAC 
CTGCAGTGGG TGGGCTCAGG GGCCCAGGGT GCTGTCTTCC TGGGGCGCTT CCATGGGGAG 
GAGGTGGCTG TGAAGAAGGT ACGAGACCTT AAAGAGACCG ACATCAAGCA CCTGCGCAAG 
CTGAAGCACC CCAACATCAT CACTTTCAAG GGTGTGTGCA CCCAGGCTCC TTGCTACTGC 
ATCCTCATGG AGTTCTGCGC CCAGGGTCAG CTATATGAGG TACTACGGGC TGGCCGCCCC 
GTCACCCCCT CCTTGCTGGT TGACTGGTCC ATGGGCATCG CTGGTGGCAT GAACTACCTG 
CACCTGCACA AGATTATCCA CAGAGACCTC AAGTCCCCTA ACATGCTAAT CACATATGAC 
GACGTGGTGA AGATCTCAGA TTTTGGCACT TCCAAGGAGC TGAGTGACAA GAGCACCAAG 
ATGTCCTTTG CAGGGACAGT AGCCTGGATG GCCCCTGAGG TGATCCGCAA TGAGCCTGTG 
TCTGAGAAGG TCGACATCTG GTCCTTTGGT GTAGTGTTGT GGGAACTGCT GACTGGAGAG 
ATCCCCTACA AAGACGTAGA TTCCTCAGCC ATCATCTGGG GTGTAGGAAG CAACAGTCTC 
CATCTGCCTG TGCCCTCCAG CTGCCCAGAT GGCTTCAAAA TCCTGCTTCG CCAGTGCTGG 
AATAGCAAAC CACGAAATCG TCCATCATTC CGACAGATCC TGTTGCATCT GGACATTGCC 
TCAGCTGATG TACTCTCCAC ACCCCAGGAG ACTTACTTTA AGTCCCAGGC AGAGTGGCGG 
GAAGAAGTAA AGCTGCACTT TGAAAAGATT AAGTCAGAAG GGACCTGTCT GCACCGCCTA 
GAAGAGGAGC TGGTGATGCG GAGGAGGGAG GAGCTCAGAC ATGCATTGGA TATCAGGGAG 
CACTACGAAC GCAAGCTGGA GAGAGCCAAC AACCTGTACA TGGAGTTAAA TGCCCTCATG 
TTGCAGCTGG AACTCAAGGA ACGAGAGTTG CTCAGGCGGG AGCAAGCTTT AGAGCGGAGG 
TGCCCAGGCC TACTTAAGTC ACACCCTTCC CGGGGCCTTC TGCATGGGAA CACAATGGAG 
AAGCTCATTA AGAAGAGGAA CATGCCACAG AAGCTCTCAC CCCATAGCAA AAGGCCAGAT 
ATCCTCAAGA CAGAGTCTTT GCTACCTAAA CTAGATGCAG CCCTAAGTGG GGTGGGGCTT 
CCTGGGTGTC CTAAGGGCCC CCCCTCACCA GGACGGAGTC GCCGTGGCAA GACCCGTCAC 
CGCAAGGCCA GCGCCAAGGG CAGCTGTGGG GACCTGCCTG GGCTTCGTGC AGCTATGCCA 
CCCCATGAAC CTGGGGGACC AGGAAGCCCA GGGGGAGTGG GACCCTCAGC CTGGGAGGCC 
TGTCCCCCCA CCCTCTGTGG GCTCCATCAT GACCTTCTGC TCCGCAAGAT GTCTTCGTCA 
TCCCCAGACC TGCTGTCAGC AGCACTGGGG TCCCGGAGCC GGGGAGCCAC AGGAGGAGCT 
GGGGATCCTG GCTCACCACC TCCAGCCCGG GGTGACACCC CCCCAAGTGA GGGCTCAGCC 
CCTGGCTCCA CCAGCCCAGA TTCACCTGGG GGAGCCAAAG GGGAGCCACC TCCACCAGTA 
GGACCTGGTG AAGGTGTGGG GCTGCTGGGA ACTGGAAGGG AAGGGACTTC GGGCCGGGGA 
GGAAGCCGGG CTGGGTCCCA GCACTTGACC CCAGCTGCAC TCCTGTACAG GGCTGCTGTC 
ACCCGCAGTC AGAAACGAGG CATCTCTTCA GAAGAGGAGG AAGGAGAGGT GGACAGTGAA 
GTAGAACTGA CTTCAAGCCA GAGGTGGCCT CAAGGCCTGA ACATGCGCCA GTCACTATCT 
ACCTTCAGCT CAGAGAATCC ATCAGATGGG GAGGAAGGTA CAGCTAGTGA GCCTTCCCCC 
AGCGGCACCC CTGAAGTTGG CAGTACCAAC ACTGATGAGC GGCCCGATGA ACGGTCTGAT 
GACATGTGCT CCCAGGGCTC AGAAATCCCA CTGGACCCAC CTCCTTTGGA GGTGGTCCCT 
GACCCTGTAC CCAGCACCGT GCCCATGCCA CACCAGGACC TACTCAGAGG GGAGCAGGGC 
CCTCCCAATA CTGAGGACTC AGACTGTGAC AGCACTGAAT TGGACAACTC CAACAGCATT 
GATGCCTTGC GGCCCCCAGC CTCCCTCCCT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K12 ( XM_012653246.1 ) cDNA ORF clone, Propithecus coquereli(Coquerel's sifaka) -> XP_012508700.1 Propithecus coquereli mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPr96687
Clone ID Related Accession (Same CDS sequence)	XM_012653246.1
Accession Version	XM_012653246.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-31
Organism	Propithecus coquereli(Coquerel's sifaka)
Product	mitogen-activated protein kinase kinase kinase 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012135081.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Propithecus coquereli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGCC TCCATGAGAC CCGAACACCC TCCCCTTCCT TTGGGGGCTT CGTGTCTACC 
CTAAGCGAGG CATCCATGCG CAAGCTGGAC CCAGACACTT CCGACTGCAC TCCCGAAAAG 
GACCTGACGC CTACCCATGT CCTGCAGCTA CATGAGCAGG ACACAGGGGG CCCAGGGGGA 
GCCCCTGGGT CACCTGAGAG TCGGGCGTCC AGAGTTCGAG CAGATGAGGT GCGGCTACAG 
TGCCAGAGTG GCAGTGGCTT TCTTGAAGGC CTCTTTGGCT GCCTGCGCCC TGTCTGGACC 
ATGATTGGCA AAGCCTATTC CACCGAGCAC AAGCAGCAGC AGGAAGACCT TTGGGAGGTC 
CCCTTTGAGG AAATCCTGGA CCTGCAGTGG GTGGGCTCAG GGGCCCAGGG TGCTGTCTTC 
CTGGGGCGCT TCCATGGGGA GGAGGTGGCT GTGAAGAAGG TACGAGACCT TAAAGAGACC 
GACATCAAGC ACCTGCGCAA GCTGAAGCAC CCCAACATCA TCACTTTCAA GGGTGTGTGC 
ACCCAGGCTC CTTGCTACTG CATCCTCATG GAGTTCTGCG CCCAGGGTCA GCTATATGAG 
GTACTACGGG CTGGCCGCCC CGTCACCCCC TCCTTGCTGG TTGACTGGTC CATGGGCATC 
GCTGGTGGCA TGAACTACCT GCACCTGCAC AAGATTATCC ACAGAGACCT CAAGTCCCCT 
AACATGCTAA TCACATATGA CGACGTGGTG AAGATCTCAG ATTTTGGCAC TTCCAAGGAG 
CTGAGTGACA AGAGCACCAA GATGTCCTTT GCAGGGACAG TAGCCTGGAT GGCCCCTGAG 
GTGATCCGCA ATGAGCCTGT GTCTGAGAAG GTCGACATCT GGTCCTTTGG TGTAGTGTTG 
TGGGAACTGC TGACTGGAGA GATCCCCTAC AAAGACGTAG ATTCCTCAGC CATCATCTGG 
GGTGTAGGAA GCAACAGTCT CCATCTGCCT GTGCCCTCCA GCTGCCCAGA TGGCTTCAAA 
ATCCTGCTTC GCCAGTGCTG GAATAGCAAA CCACGAAATC GTCCATCATT CCGACAGATC 
CTGTTGCATC TGGACATTGC CTCAGCTGAT GTACTCTCCA CACCCCAGGA GACTTACTTT 
AAGTCCCAGG CAGAGTGGCG GGAAGAAGTA AAGCTGCACT TTGAAAAGAT TAAGTCAGAA 
GGGACCTGTC TGCACCGCCT AGAAGAGGAG CTGGTGATGC GGAGGAGGGA GGAGCTCAGA 
CATGCATTGG ATATCAGGGA GCACTACGAA CGCAAGCTGG AGAGAGCCAA CAACCTGTAC 
ATGGAGTTAA ATGCCCTCAT GTTGCAGCTG GAACTCAAGG AACGAGAGTT GCTCAGGCGG 
GAGCAAGCTT TAGAGCGGAG GTGCCCAGGC CTACTTAAGT CACACCCTTC CCGGGGCCTT 
CTGCATGGGA ACACAATGGA GAAGCTCATT AAGAAGAGGA ACATGCCACA GAAGCTCTCA 
CCCCATAGCA AAAGGCCAGA TATCCTCAAG ACAGAGTCTT TGCTACCTAA ACTAGATGCA 
GCCCTAAGTG GGGTGGGGCT TCCTGGGTGT CCTAAGGGCC CCCCCTCACC AGGACGGAGT 
CGCCGTGGCA AGACCCGTCA CCGCAAGGCC AGCGCCAAGG GCAGCTGTGG GGACCTGCCT 
GGGCTTCGTG CAGCTATGCC ACCCCATGAA CCTGGGGGAC CAGGAAGCCC AGGGGGAGTG 
GGACCCTCAG CCTGGGAGGC CTGTCCCCCC ACCCTCTGTG GGCTCCATCA TGACCTTCTG 
CTCCGCAAGA TGTCTTCGTC ATCCCCAGAC CTGCTGTCAG CAGCACTGGG GTCCCGGAGC 
CGGGGAGCCA CAGGAGGAGC TGGGGATCCT GGCTCACCAC CTCCAGCCCG GGGTGACACC 
CCCCCAAGTG AGGGCTCAGC CCCTGGCTCC ACCAGCCCAG ATTCACCTGG GGGAGCCAAA 
GGGGAGCCAC CTCCACCAGT AGGACCTGGT GAAGGTGTGG GGCTGCTGGG AACTGGAAGG 
GAAGGGACTT CGGGCCGGGG AGGAAGCCGG GCTGGGTCCC AGCACTTGAC CCCAGCTGCA 
CTCCTGTACA GGGCTGCTGT CACCCGCAGT CAGAAACGAG GCATCTCTTC AGAAGAGGAG 
GAAGGAGAGG TGGACAGTGA AGTAGAACTG ACTTCAAGCC AGAGGTGGCC TCAAGGCCTG 
AACATGCGCC AGTCACTATC TACCTTCAGC TCAGAGAATC CATCAGATGG GGAGGAAGGT 
ACAGCTAGTG AGCCTTCCCC CAGCGGCACC CCTGAAGTTG GCAGTACCAA CACTGATGAG 
CGGCCCGATG AACGGTCTGA TGACATGTGC TCCCAGGGCT CAGAAATCCC ACTGGACCCA 
CCTCCTTTGG AGGTGGTCCC TGACCCTGTA CCCAGCACCG TGCCCATGCC ACACCAGGAC 
CTACTCAGAG GGGAGCAGGG CCCTCCCAAT ACTGAGGACT CAGACTGTGA CAGCACTGAA 
TTGGACAACT CCAACAGCAT TGATGCCTTG CGGCCCCCAG CCTCCCTCCC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012508700.1
CDS	273..2849
Translation	MACLHETRTPSPSFGGFVSTLSEASMRKLDPDTSDCTPEKDLTPTHVLQLHEQDTGGPGGAPGSPESRASRVRADEVRLQCQSGSGFLEGLFGCLRPVWTMIGKAYSTEHKQQQEDLWEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKETDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASADVLSTPQETYFKSQAEWREEVKLHFEKIKSEGTCLHRLEEELVMRRREELRHALDIREHYERKLERANNLYMELNALMLQLELKERELLRREQALERRCPGLLKSHPSRGLLHGNTMEKLIKKRNMPQKLSPHSKRPDILKTESLLPKLDAALSGVGLPGCPKGPPSPGRSRRGKTRHRKASAKGSCGDLPGLRAAMPPHEPGGPGSPGGVGPSAWEACPPTLCGLHHDLLLRKMSSSSPDLLSAALGSRSRGATGGAGDPGSPPPARGDTPPSEGSAPGSTSPDSPGGAKGEPPPPVGPGEGVGLLGTGREGTSGRGGSRAGSQHLTPAALLYRAAVTRSQKRGISSEEEEGEVDSEVELTSSQRWPQGLNMRQSLSTFSSENPSDGEEGTASEPSPSGTPEVGSTNTDERPDERSDDMCSQGSEIPLDPPPLEVVPDPVPSTVPMPHQDLLRGEQGPPNTEDSDCDSTELDNSNSIDALRPPASLPP

Target ORF information:

RefSeq Version	XM_012653246.1
Organism	Propithecus coquereli(Coquerel's sifaka)
Definition	Propithecus coquereli mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012653246.1

ORF Insert Sequence:

ATGGCCTGCC TCCATGAGAC CCGAACACCC TCCCCTTCCT TTGGGGGCTT CGTGTCTACC 
CTAAGCGAGG CATCCATGCG CAAGCTGGAC CCAGACACTT CCGACTGCAC TCCCGAAAAG 
GACCTGACGC CTACCCATGT CCTGCAGCTA CATGAGCAGG ACACAGGGGG CCCAGGGGGA 
GCCCCTGGGT CACCTGAGAG TCGGGCGTCC AGAGTTCGAG CAGATGAGGT GCGGCTACAG 
TGCCAGAGTG GCAGTGGCTT TCTTGAAGGC CTCTTTGGCT GCCTGCGCCC TGTCTGGACC 
ATGATTGGCA AAGCCTATTC CACCGAGCAC AAGCAGCAGC AGGAAGACCT TTGGGAGGTC 
CCCTTTGAGG AAATCCTGGA CCTGCAGTGG GTGGGCTCAG GGGCCCAGGG TGCTGTCTTC 
CTGGGGCGCT TCCATGGGGA GGAGGTGGCT GTGAAGAAGG TACGAGACCT TAAAGAGACC 
GACATCAAGC ACCTGCGCAA GCTGAAGCAC CCCAACATCA TCACTTTCAA GGGTGTGTGC 
ACCCAGGCTC CTTGCTACTG CATCCTCATG GAGTTCTGCG CCCAGGGTCA GCTATATGAG 
GTACTACGGG CTGGCCGCCC CGTCACCCCC TCCTTGCTGG TTGACTGGTC CATGGGCATC 
GCTGGTGGCA TGAACTACCT GCACCTGCAC AAGATTATCC ACAGAGACCT CAAGTCCCCT 
AACATGCTAA TCACATATGA CGACGTGGTG AAGATCTCAG ATTTTGGCAC TTCCAAGGAG 
CTGAGTGACA AGAGCACCAA GATGTCCTTT GCAGGGACAG TAGCCTGGAT GGCCCCTGAG 
GTGATCCGCA ATGAGCCTGT GTCTGAGAAG GTCGACATCT GGTCCTTTGG TGTAGTGTTG 
TGGGAACTGC TGACTGGAGA GATCCCCTAC AAAGACGTAG ATTCCTCAGC CATCATCTGG 
GGTGTAGGAA GCAACAGTCT CCATCTGCCT GTGCCCTCCA GCTGCCCAGA TGGCTTCAAA 
ATCCTGCTTC GCCAGTGCTG GAATAGCAAA CCACGAAATC GTCCATCATT CCGACAGATC 
CTGTTGCATC TGGACATTGC CTCAGCTGAT GTACTCTCCA CACCCCAGGA GACTTACTTT 
AAGTCCCAGG CAGAGTGGCG GGAAGAAGTA AAGCTGCACT TTGAAAAGAT TAAGTCAGAA 
GGGACCTGTC TGCACCGCCT AGAAGAGGAG CTGGTGATGC GGAGGAGGGA GGAGCTCAGA 
CATGCATTGG ATATCAGGGA GCACTACGAA CGCAAGCTGG AGAGAGCCAA CAACCTGTAC 
ATGGAGTTAA ATGCCCTCAT GTTGCAGCTG GAACTCAAGG AACGAGAGTT GCTCAGGCGG 
GAGCAAGCTT TAGAGCGGAG GTGCCCAGGC CTACTTAAGT CACACCCTTC CCGGGGCCTT 
CTGCATGGGA ACACAATGGA GAAGCTCATT AAGAAGAGGA ACATGCCACA GAAGCTCTCA 
CCCCATAGCA AAAGGCCAGA TATCCTCAAG ACAGAGTCTT TGCTACCTAA ACTAGATGCA 
GCCCTAAGTG GGGTGGGGCT TCCTGGGTGT CCTAAGGGCC CCCCCTCACC AGGACGGAGT 
CGCCGTGGCA AGACCCGTCA CCGCAAGGCC AGCGCCAAGG GCAGCTGTGG GGACCTGCCT 
GGGCTTCGTG CAGCTATGCC ACCCCATGAA CCTGGGGGAC CAGGAAGCCC AGGGGGAGTG 
GGACCCTCAG CCTGGGAGGC CTGTCCCCCC ACCCTCTGTG GGCTCCATCA TGACCTTCTG 
CTCCGCAAGA TGTCTTCGTC ATCCCCAGAC CTGCTGTCAG CAGCACTGGG GTCCCGGAGC 
CGGGGAGCCA CAGGAGGAGC TGGGGATCCT GGCTCACCAC CTCCAGCCCG GGGTGACACC 
CCCCCAAGTG AGGGCTCAGC CCCTGGCTCC ACCAGCCCAG ATTCACCTGG GGGAGCCAAA 
GGGGAGCCAC CTCCACCAGT AGGACCTGGT GAAGGTGTGG GGCTGCTGGG AACTGGAAGG 
GAAGGGACTT CGGGCCGGGG AGGAAGCCGG GCTGGGTCCC AGCACTTGAC CCCAGCTGCA 
CTCCTGTACA GGGCTGCTGT CACCCGCAGT CAGAAACGAG GCATCTCTTC AGAAGAGGAG 
GAAGGAGAGG TGGACAGTGA AGTAGAACTG ACTTCAAGCC AGAGGTGGCC TCAAGGCCTG 
AACATGCGCC AGTCACTATC TACCTTCAGC TCAGAGAATC CATCAGATGG GGAGGAAGGT 
ACAGCTAGTG AGCCTTCCCC CAGCGGCACC CCTGAAGTTG GCAGTACCAA CACTGATGAG 
CGGCCCGATG AACGGTCTGA TGACATGTGC TCCCAGGGCT CAGAAATCCC ACTGGACCCA 
CCTCCTTTGG AGGTGGTCCC TGACCCTGTA CCCAGCACCG TGCCCATGCC ACACCAGGAC 
CTACTCAGAG GGGAGCAGGG CCCTCCCAAT ACTGAGGACT CAGACTGTGA CAGCACTGAA 
TTGGACAACT CCAACAGCAT TGATGCCTTG CGGCCCCCAG CCTCCCTCCC TCCATGA