LOC7458009 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC7458009
Entrez Gene ID	7458009
Full Name	glycosyl hydrolase family 38 family protein
Synonyms	POPTRDRAFT_572859
Gene Type	protein-coding
Organism	Populus trichocarpa(black cottonwood)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024585099.1	XP_024440867.1	probable alpha-mannosidase At5g13980 isoform X2
XM_002321039.2	XP_002321075.2	glycosyl hydrolase family 38 family protein
XM_024585098.1	XP_024440866.1	probable alpha-mannosidase At5g13980 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, D?jardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasj?rvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Lepl? JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouz? P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC7458009 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC7458009 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa443205	XM_024585099.1 Latest version!	Populus trichocarpa probable alpha-mannosidase At5g13980 (LOC7458009), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPa443204	XM_024585098.1 Latest version!	Populus trichocarpa probable alpha-mannosidase At5g13980 (LOC7458009), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPa29328	XM_002321039.2 Latest version!	Populus trichocarpa glycosyl hydrolase family 38 family protein (POPTR_0014s14050g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC7458009 ( XM_024585099.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024440867.1 Populus trichocarpa probable alpha-mannosidase At5g13980 (LOC7458009), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa443205
Clone ID Related Accession (Same CDS sequence)	XM_024585099.1
Accession Version	XM_024585099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	probable alpha-mannosidase At5g13980 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037298.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCATGC ATGATGAAGC GGTGACACAT TATATTGACA TGATTGACCA GACAACTCTT 
GGGCATCGGT TTATCAAAAA GGATTTTGGT GTGACTCCAA GAGTTGGTTG GCAAATTGAT 
CCTTTTGGTC ATTCTGCGGT GCAGGCTTAC ATGTTGGGAG CGGAAATTGG ATTCGACTCT 
CTTTTCTTTG GCCGGATAGA TTATCAAGAC CGGGCCAAGC GTAAAAATGA GAAAAGTCTT 
GAAGTTGTCT GGCAGGCTTC CAAGAGTTTT GGTTCGTCTG CACAGATTTT TGCTGGTGCT 
TTCCCGGAAC ACTATGAGCC TCCTCCTGGT GGTTTCTATT TTGAAGTCAA TGACCCTTCA 
CCTGTTGTTC AAGATGACAT CAATTTGTTT GATTACAATG TTCAAGAACG AGTCGATGAC 
TTCGTTGCTG CTGCAGTATC ACAGGCTAAT ATAACACGAA CAAATCATAT AATGTGGACC 
ATGGGAACAG ATTTTAAGTA TCAGTATGCA CATTCATGGT TCCGACAGAT GGACAAGCTG 
ATTCATTATG TCAACATGGA TGGACGTGTT AATGCTCTCT ATTCTACACC ATCCATTTAC 
ACTGATGCAA AACATGCTAC TAATGAGCAC TGGCCAGTAA AGACCGGGGA CTTTTTCCCG 
TATGCAGATC GTGCAAATGG TTACTGGACT GGATACTTTG CAAGTAGACC TGCCTTGAAA 
CGCTATGTCA GGATGATGAG TGGCTACTAC TTGGCTGCAA GACAATTAGA GTTTTACAAT 
GGGAGGAGCA ATAGAGGACC GAACACAGAC TCATTGGCAG ATTCTCTTGC CATTGCTCAG 
CATCATGACG CTGTTACTGG TACAGAAAAG CAACATGTGG CTAATGATTA TGCAAAACGA 
TTGTCAATTG GCTACACAGA GGCTGAAAAG TTAGTTGCAT CCTCACTTGC TTGCTTGGTG 
GAGTCTGCAT CACATACTGG ATGTCAGAGG TCAACTACTA AATTTCAACA GTGCCCACTT 
CTGAATATTA GTTACTGTCC TGCATCAGAA GTAGATCTAT CTCAAGGGAG AAACTTGATT 
GTTGTTGTCT ACAATGCACT TGGATGGGCG AGGGATGATG TAATTCAATT TCCTGTCTTC 
AATGAAAATG TTATTGTGCA TGATTCTGAG AAAAGAGAAA TCGTGTCACA AATTATTCCA 
ATAGCAGATG CCTTTGTGGG CTTGAGGAAC TCACATGTTA ATGCATACTT GGGTAGATCC 
CCAGTGGAGA CGCCTAAGTA TTGGCTTGCG TTTCCAGTTT CTGTTCCACC TTTTGGTTTC 
AGCACTTATT CCATCTCAAG TGCCAAAAGG GCAGGTGCTC ATTCGAGTAA ATCATCTGTA 
TACACATTAA GAAGTGAGAA ATCTGCTGTT GAAGTTGGCC AAGGAAATTT GAAGCTTACA 
TTTTCTGCAG ATAAAATCAA GCATGCTAAT TATGTTAACA GCAGAAGCTC AGTTAAGGAA 
TCAGTTGAAC AGACATTCAG TTTTTATGCT GGATACAATG GAACTGGAAA TGATAAAGAT 
CCACAGAATT CTGGGGCATA TATCTTCCGT CCAAATGGAA CATTTCCTAT AAACCCTGAA 
TCCCAGGTTC CTTTGACTGT TATGCGTGGA CCTGTTTTAG ATGAAGTACA TCAACAGGTC 
AGCCAATGGA TATATCAGAT CACCAGATTA TACAAGGGGA GAGAGCATGT TGAAGTTGAG 
TTTATCGTTG GGCCCATACC CATTGAAGAT GGAATTGGAA AAGAAGTTGC AACCCAAATC 
ACAACAACCA TGGAAACCAA TAAAACATTT TACACAGATT CTAATGGACG TGATTTTATC 
AAAAGGATTC GTGATTATAG AGCAGATTGG GACCTGGAAG TGAATCAACC ATTTGCTGGA 
AATTATTACC CAATTAATCT TGGAATTTAT TTCCAAGATG ACAAGAAGGA ATTTTCAGTT 
CTGGTGGATC GAGCACTTGG AGGATCTAGT CTGGTGGATG GACAAATAGA GCTGATGCTC 
CATAGGAGAC TGCTACTGGA TGATTCCAGG GGTGTTGCGG AGGCTCTTAA TGAAACAGTA 
TGTGTCCTTG ATCAATGCAA GGGATTGACG ATTCAAGGAA AATATTATTA CAGAATTGAC 
CCTATAGGAG AAGGTGCTAA ATGGCGTCGC TCATTCGGCC AGGAGATATA TTCACCCCTC 
CTCCTTGCGT TTTCTGAAGA GGATGGAGAT AATTGGATGA ATTCTCATGT GACCACATTT 
TCTGGCTTTG ATTCTTCCTA CATTTTGCCT GATAATGTGG CAGTTTTAAC TCTCCAGGAG 
CTTGATGATG GAAAAGTTCT TCTCCGTTTA GCTCATTTAT ATGAGATGGG AGAGGACAAG 
GATCTTTCAG TGATGACAAG CGTAGAACTT AGAAAGTTGT TCCCGAAAAA GAAGATCGGC 
AAAGCTGCAG AAATGAGCTT ATCTGCCAAT CAAGAAAGGG CTGAAATGGA GAAAAAGAGG 
CTTGTGTGGA AAGCAGAAGG ATCATCAAGA AAACAAGCAG TGCTGAGGGG TGGACCTGTT 
GATCCTGCAA AACTGGTGGT GGAACTTGCT CCGATGGAAA TCCGCACATT TGTTATTGAC 
TTCGATCACC AGTTCCACCA TGTTTTTGCT GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024440867.1
CDS	112..2787
Translation	MCMHDEAVTHYIDMIDQTTLGHRFIKKDFGVTPRVGWQIDPFGHSAVQAYMLGAEIGFDSLFFGRIDYQDRAKRKNEKSLEVVWQASKSFGSSAQIFAGAFPEHYEPPPGGFYFEVNDPSPVVQDDINLFDYNVQERVDDFVAAAVSQANITRTNHIMWTMGTDFKYQYAHSWFRQMDKLIHYVNMDGRVNALYSTPSIYTDAKHATNEHWPVKTGDFFPYADRANGYWTGYFASRPALKRYVRMMSGYYLAARQLEFYNGRSNRGPNTDSLADSLAIAQHHDAVTGTEKQHVANDYAKRLSIGYTEAEKLVASSLACLVESASHTGCQRSTTKFQQCPLLNISYCPASEVDLSQGRNLIVVVYNALGWARDDVIQFPVFNENVIVHDSEKREIVSQIIPIADAFVGLRNSHVNAYLGRSPVETPKYWLAFPVSVPPFGFSTYSISSAKRAGAHSSKSSVYTLRSEKSAVEVGQGNLKLTFSADKIKHANYVNSRSSVKESVEQTFSFYAGYNGTGNDKDPQNSGAYIFRPNGTFPINPESQVPLTVMRGPVLDEVHQQVSQWIYQITRLYKGREHVEVEFIVGPIPIEDGIGKEVATQITTTMETNKTFYTDSNGRDFIKRIRDYRADWDLEVNQPFAGNYYPINLGIYFQDDKKEFSVLVDRALGGSSLVDGQIELMLHRRLLLDDSRGVAEALNETVCVLDQCKGLTIQGKYYYRIDPIGEGAKWRRSFGQEIYSPLLLAFSEEDGDNWMNSHVTTFSGFDSSYILPDNVAVLTLQELDDGKVLLRLAHLYEMGEDKDLSVMTSVELRKLFPKKKIGKAAEMSLSANQERAEMEKKRLVWKAEGSSRKQAVLRGGPVDPAKLVVELAPMEIRTFVIDFDHQFHHVFAA

Target ORF information:

RefSeq Version	XM_024585099.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable alpha-mannosidase At5g13980 (LOC7458009), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024585099.1

ORF Insert Sequence:

ATGTGCATGC ATGATGAAGC GGTGACACAT TATATTGACA TGATTGACCA GACAACTCTT 
GGGCATCGGT TTATCAAAAA GGATTTTGGT GTGACTCCAA GAGTTGGTTG GCAAATTGAT 
CCTTTTGGTC ATTCTGCGGT GCAGGCTTAC ATGTTGGGAG CGGAAATTGG ATTCGACTCT 
CTTTTCTTTG GCCGGATAGA TTATCAAGAC CGGGCCAAGC GTAAAAATGA GAAAAGTCTT 
GAAGTTGTCT GGCAGGCTTC CAAGAGTTTT GGTTCGTCTG CACAGATTTT TGCTGGTGCT 
TTCCCGGAAC ACTATGAGCC TCCTCCTGGT GGTTTCTATT TTGAAGTCAA TGACCCTTCA 
CCTGTTGTTC AAGATGACAT CAATTTGTTT GATTACAATG TTCAAGAACG AGTCGATGAC 
TTCGTTGCTG CTGCAGTATC ACAGGCTAAT ATAACACGAA CAAATCATAT AATGTGGACC 
ATGGGAACAG ATTTTAAGTA TCAGTATGCA CATTCATGGT TCCGACAGAT GGACAAGCTG 
ATTCATTATG TCAACATGGA TGGACGTGTT AATGCTCTCT ATTCTACACC ATCCATTTAC 
ACTGATGCAA AACATGCTAC TAATGAGCAC TGGCCAGTAA AGACCGGGGA CTTTTTCCCG 
TATGCAGATC GTGCAAATGG TTACTGGACT GGATACTTTG CAAGTAGACC TGCCTTGAAA 
CGCTATGTCA GGATGATGAG TGGCTACTAC TTGGCTGCAA GACAATTAGA GTTTTACAAT 
GGGAGGAGCA ATAGAGGACC GAACACAGAC TCATTGGCAG ATTCTCTTGC CATTGCTCAG 
CATCATGACG CTGTTACTGG TACAGAAAAG CAACATGTGG CTAATGATTA TGCAAAACGA 
TTGTCAATTG GCTACACAGA GGCTGAAAAG TTAGTTGCAT CCTCACTTGC TTGCTTGGTG 
GAGTCTGCAT CACATACTGG ATGTCAGAGG TCAACTACTA AATTTCAACA GTGCCCACTT 
CTGAATATTA GTTACTGTCC TGCATCAGAA GTAGATCTAT CTCAAGGGAG AAACTTGATT 
GTTGTTGTCT ACAATGCACT TGGATGGGCG AGGGATGATG TAATTCAATT TCCTGTCTTC 
AATGAAAATG TTATTGTGCA TGATTCTGAG AAAAGAGAAA TCGTGTCACA AATTATTCCA 
ATAGCAGATG CCTTTGTGGG CTTGAGGAAC TCACATGTTA ATGCATACTT GGGTAGATCC 
CCAGTGGAGA CGCCTAAGTA TTGGCTTGCG TTTCCAGTTT CTGTTCCACC TTTTGGTTTC 
AGCACTTATT CCATCTCAAG TGCCAAAAGG GCAGGTGCTC ATTCGAGTAA ATCATCTGTA 
TACACATTAA GAAGTGAGAA ATCTGCTGTT GAAGTTGGCC AAGGAAATTT GAAGCTTACA 
TTTTCTGCAG ATAAAATCAA GCATGCTAAT TATGTTAACA GCAGAAGCTC AGTTAAGGAA 
TCAGTTGAAC AGACATTCAG TTTTTATGCT GGATACAATG GAACTGGAAA TGATAAAGAT 
CCACAGAATT CTGGGGCATA TATCTTCCGT CCAAATGGAA CATTTCCTAT AAACCCTGAA 
TCCCAGGTTC CTTTGACTGT TATGCGTGGA CCTGTTTTAG ATGAAGTACA TCAACAGGTC 
AGCCAATGGA TATATCAGAT CACCAGATTA TACAAGGGGA GAGAGCATGT TGAAGTTGAG 
TTTATCGTTG GGCCCATACC CATTGAAGAT GGAATTGGAA AAGAAGTTGC AACCCAAATC 
ACAACAACCA TGGAAACCAA TAAAACATTT TACACAGATT CTAATGGACG TGATTTTATC 
AAAAGGATTC GTGATTATAG AGCAGATTGG GACCTGGAAG TGAATCAACC ATTTGCTGGA 
AATTATTACC CAATTAATCT TGGAATTTAT TTCCAAGATG ACAAGAAGGA ATTTTCAGTT 
CTGGTGGATC GAGCACTTGG AGGATCTAGT CTGGTGGATG GACAAATAGA GCTGATGCTC 
CATAGGAGAC TGCTACTGGA TGATTCCAGG GGTGTTGCGG AGGCTCTTAA TGAAACAGTA 
TGTGTCCTTG ATCAATGCAA GGGATTGACG ATTCAAGGAA AATATTATTA CAGAATTGAC 
CCTATAGGAG AAGGTGCTAA ATGGCGTCGC TCATTCGGCC AGGAGATATA TTCACCCCTC 
CTCCTTGCGT TTTCTGAAGA GGATGGAGAT AATTGGATGA ATTCTCATGT GACCACATTT 
TCTGGCTTTG ATTCTTCCTA CATTTTGCCT GATAATGTGG CAGTTTTAAC TCTCCAGGAG 
CTTGATGATG GAAAAGTTCT TCTCCGTTTA GCTCATTTAT ATGAGATGGG AGAGGACAAG 
GATCTTTCAG TGATGACAAG CGTAGAACTT AGAAAGTTGT TCCCGAAAAA GAAGATCGGC 
AAAGCTGCAG AAATGAGCTT ATCTGCCAAT CAAGAAAGGG CTGAAATGGA GAAAAAGAGG 
CTTGTGTGGA AAGCAGAAGG ATCATCAAGA AAACAAGCAG TGCTGAGGGG TGGACCTGTT 
GATCCTGCAA AACTGGTGGT GGAACTTGCT CCGATGGAAA TCCGCACATT TGTTATTGAC 
TTCGATCACC AGTTCCACCA TGTTTTTGCT GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7458009 ( XM_024585098.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024440866.1 Populus trichocarpa probable alpha-mannosidase At5g13980 (LOC7458009), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa443204
Clone ID Related Accession (Same CDS sequence)	XM_024585098.1
Accession Version	XM_024585098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	probable alpha-mannosidase At5g13980 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037298.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAG TGTTGATGGT GGTTTTGTTG CTGTTGCTGC TGGGTGGTTC ATTATTTGTG 
GTGGAAGCCA AGTACATGGT TTATAACACT TCACAAGGTA TAGTGAAAGA TAAGATCAAT 
GTTCACTTGG TCGCTCACAC CCATGATGAT GTTGGTTGGT TGAAGACTGT TGATCAGTAC 
TATGTTGGTT CTAACAATTC TATCCAAGGT GCTTGTGTGC AAAATGTATT GGATTCTCTT 
ATTCCTGCGC TGTTGGCTGA CAAGAACCGG AAATTCATTT ATGTTGAACA GGCTTTTTTC 
CAACGGTGGT GGAGAGATCA GAGCGAGACT ATGCAGCATG TAGTCAAGCA ACTTGTTAGT 
TCCGGTCAAC TTGAATTCAT AAATGGGGGG ATGTGCATGC ATGATGAAGC GGTGACACAT 
TATATTGACA TGATTGACCA GACAACTCTT GGGCATCGGT TTATCAAAAA GGATTTTGGT 
GTGACTCCAA GAGTTGGTTG GCAAATTGAT CCTTTTGGTC ATTCTGCGGT GCAGGCTTAC 
ATGTTGGGAG CGGAAATTGG ATTCGACTCT CTTTTCTTTG GCCGGATAGA TTATCAAGAC 
CGGGCCAAGC GTAAAAATGA GAAAAGTCTT GAAGTTGTCT GGCAGGCTTC CAAGAGTTTT 
GGTTCGTCTG CACAGATTTT TGCTGGTGCT TTCCCGGAAC ACTATGAGCC TCCTCCTGGT 
GGTTTCTATT TTGAAGTCAA TGACCCTTCA CCTGTTGTTC AAGATGACAT CAATTTGTTT 
GATTACAATG TTCAAGAACG AGTCGATGAC TTCGTTGCTG CTGCAGTATC ACAGGCTAAT 
ATAACACGAA CAAATCATAT AATGTGGACC ATGGGAACAG ATTTTAAGTA TCAGTATGCA 
CATTCATGGT TCCGACAGAT GGACAAGCTG ATTCATTATG TCAACATGGA TGGACGTGTT 
AATGCTCTCT ATTCTACACC ATCCATTTAC ACTGATGCAA AACATGCTAC TAATGAGCAC 
TGGCCAGTAA AGACCGGGGA CTTTTTCCCG TATGCAGATC GTGCAAATGG TTACTGGACT 
GGATACTTTG CAAGTAGACC TGCCTTGAAA CGCTATGTCA GGATGATGAG TGGCTACTAC 
TTGGCTGCAA GACAATTAGA GTTTTACAAT GGGAGGAGCA ATAGAGGACC GAACACAGAC 
TCATTGGCAG ATTCTCTTGC CATTGCTCAG CATCATGACG CTGTTACTGG TACAGAAAAG 
CAACATGTGG CTAATGATTA TGCAAAACGA TTGTCAATTG GCTACACAGA GGCTGAAAAG 
TTAGTTGCAT CCTCACTTGC TTGCTTGGTG GAGTCTGCAT CACATACTGG ATGTCAGAGG 
TCAACTACTA AATTTCAACA GTGCCCACTT CTGAATATTA GTTACTGTCC TGCATCAGAA 
GTAGATCTAT CTCAAGGGAG AAACTTGATT GTTGTTGTCT ACAATGCACT TGGATGGGCG 
AGGGATGATG TAATTCAATT TCCTGTCTTC AATGAAAATG TTATTGTGCA TGATTCTGAG 
AAAAGAGAAA TCGTGTCACA AATTATTCCA ATAGCAGATG CCTTTGTGGG CTTGAGGAAC 
TCACATGTTA ATGCATACTT GGGTAGATCC CCAGTGGAGA CGCCTAAGTA TTGGCTTGCG 
TTTCCAGTTT CTGTTCCACC TTTTGGTTTC AGCACTTATT CCATCTCAAG TGCCAAAAGG 
GCAGGTGCTC ATTCGAGTAA ATCATCTGTA TACACATTAA GAAGTGAGAA ATCTGCTGTT 
GAAGTTGGCC AAGGAAATTT GAAGCTTACA TTTTCTGCAG ATAAAATCAA GCATGCTAAT 
TATGTTAACA GCAGAAGCTC AGTTAAGGAA TCAGTTGAAC AGACATTCAG TTTTTATGCT 
GGATACAATG GAACTGGAAA TGATAAAGAT CCACAGAATT CTGGGGCATA TATCTTCCGT 
CCAAATGGAA CATTTCCTAT AAACCCTGAA TCCCAGGTTC CTTTGACTGT TATGCGTGGA 
CCTGTTTTAG ATGAAGTACA TCAACAGGTC AGCCAATGGA TATATCAGAT CACCAGATTA 
TACAAGGGGA GAGAGCATGT TGAAGTTGAG TTTATCGTTG GGCCCATACC CATTGAAGAT 
GGAATTGGAA AAGAAGTTGC AACCCAAATC ACAACAACCA TGGAAACCAA TAAAACATTT 
TACACAGATT CTAATGGACG TGATTTTATC AAAAGGATTC GTGATTATAG AGCAGATTGG 
GACCTGGAAG TGAATCAACC ATTTGCTGGA AATTATTACC CAATTAATCT TGGAATTTAT 
TTCCAAGATG ACAAGAAGGA ATTTTCAGTT CTGGTGGATC GAGCACTTGG AGGATCTAGT 
CTGGTGGATG GACAAATAGA GCTGATGCTC CATAGGAGAC TGCTACTGGA TGATTCCAGG 
GGTGTTGCGG AGGCTCTTAA TGAAACAGTA TGTGTCCTTG ATCAATGCAA GGGATTGACG 
ATTCAAGGAA AATATTATTA CAGAATTGAC CCTATAGGAG AAGGTGCTAA ATGGCGTCGC 
TCATTCGGCC AGGAGATATA TTCACCCCTC CTCCTTGCGT TTTCTGAAGA GGATGGAGAT 
AATTGGATGA ATTCTCATGT GACCACATTT TCTGGCTTTG ATTCTTCCTA CATTTTGCCT 
GATAATGTGG CAGTTTTAAC TCTCCAGGAG CTTGATGATG GAAAAGTTCT TCTCCGTTTA 
GCTCATTTAT ATGAGATGGG AGAGGACAAG GATCTTTCAG TGATGACAAG CGTAGAACTT 
AGAAAGTTGT TCCCGAAAAA GAAGATCGGC AAAGCTGCAG AAATGAGCTT ATCTGCCAAT 
CAAGAAAGGG CTGAAATGGA GAAAAAGAGG CTTGTGTGGA AAGCAGAAGG ATCATCAAGA 
AAACAAGCAG TGCTGAGGGG TGGACCTGTT GATCCTGCAA AACTGGTGGT GGAACTTGCT 
CCGATGGAAA TCCGCACATT TGTTATTGAC TTCGATCACC AGTTCCACCA TGTTTTTGCT 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024440866.1
CDS	275..3340
Translation	MMKVLMVVLLLLLLGGSLFVVEAKYMVYNTSQGIVKDKINVHLVAHTHDDVGWLKTVDQYYVGSNNSIQGACVQNVLDSLIPALLADKNRKFIYVEQAFFQRWWRDQSETMQHVVKQLVSSGQLEFINGGMCMHDEAVTHYIDMIDQTTLGHRFIKKDFGVTPRVGWQIDPFGHSAVQAYMLGAEIGFDSLFFGRIDYQDRAKRKNEKSLEVVWQASKSFGSSAQIFAGAFPEHYEPPPGGFYFEVNDPSPVVQDDINLFDYNVQERVDDFVAAAVSQANITRTNHIMWTMGTDFKYQYAHSWFRQMDKLIHYVNMDGRVNALYSTPSIYTDAKHATNEHWPVKTGDFFPYADRANGYWTGYFASRPALKRYVRMMSGYYLAARQLEFYNGRSNRGPNTDSLADSLAIAQHHDAVTGTEKQHVANDYAKRLSIGYTEAEKLVASSLACLVESASHTGCQRSTTKFQQCPLLNISYCPASEVDLSQGRNLIVVVYNALGWARDDVIQFPVFNENVIVHDSEKREIVSQIIPIADAFVGLRNSHVNAYLGRSPVETPKYWLAFPVSVPPFGFSTYSISSAKRAGAHSSKSSVYTLRSEKSAVEVGQGNLKLTFSADKIKHANYVNSRSSVKESVEQTFSFYAGYNGTGNDKDPQNSGAYIFRPNGTFPINPESQVPLTVMRGPVLDEVHQQVSQWIYQITRLYKGREHVEVEFIVGPIPIEDGIGKEVATQITTTMETNKTFYTDSNGRDFIKRIRDYRADWDLEVNQPFAGNYYPINLGIYFQDDKKEFSVLVDRALGGSSLVDGQIELMLHRRLLLDDSRGVAEALNETVCVLDQCKGLTIQGKYYYRIDPIGEGAKWRRSFGQEIYSPLLLAFSEEDGDNWMNSHVTTFSGFDSSYILPDNVAVLTLQELDDGKVLLRLAHLYEMGEDKDLSVMTSVELRKLFPKKKIGKAAEMSLSANQERAEMEKKRLVWKAEGSSRKQAVLRGGPVDPAKLVVELAPMEIRTFVIDFDHQFHHVFAA

Target ORF information:

RefSeq Version	XM_024585098.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable alpha-mannosidase At5g13980 (LOC7458009), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024585098.1

ORF Insert Sequence:

ATGATGAAAG TGTTGATGGT GGTTTTGTTG CTGTTGCTGC TGGGTGGTTC ATTATTTGTG 
GTGGAAGCCA AGTACATGGT TTATAACACT TCACAAGGTA TAGTGAAAGA TAAGATCAAT 
GTTCACTTGG TCGCTCACAC CCATGATGAT GTTGGTTGGT TGAAGACTGT TGATCAGTAC 
TATGTTGGTT CTAACAATTC TATCCAAGGT GCTTGTGTGC AAAATGTATT GGATTCTCTT 
ATTCCTGCGC TGTTGGCTGA CAAGAACCGG AAATTCATTT ATGTTGAACA GGCTTTTTTC 
CAACGGTGGT GGAGAGATCA GAGCGAGACT ATGCAGCATG TAGTCAAGCA ACTTGTTAGT 
TCCGGTCAAC TTGAATTCAT AAATGGGGGG ATGTGCATGC ATGATGAAGC GGTGACACAT 
TATATTGACA TGATTGACCA GACAACTCTT GGGCATCGGT TTATCAAAAA GGATTTTGGT 
GTGACTCCAA GAGTTGGTTG GCAAATTGAT CCTTTTGGTC ATTCTGCGGT GCAGGCTTAC 
ATGTTGGGAG CGGAAATTGG ATTCGACTCT CTTTTCTTTG GCCGGATAGA TTATCAAGAC 
CGGGCCAAGC GTAAAAATGA GAAAAGTCTT GAAGTTGTCT GGCAGGCTTC CAAGAGTTTT 
GGTTCGTCTG CACAGATTTT TGCTGGTGCT TTCCCGGAAC ACTATGAGCC TCCTCCTGGT 
GGTTTCTATT TTGAAGTCAA TGACCCTTCA CCTGTTGTTC AAGATGACAT CAATTTGTTT 
GATTACAATG TTCAAGAACG AGTCGATGAC TTCGTTGCTG CTGCAGTATC ACAGGCTAAT 
ATAACACGAA CAAATCATAT AATGTGGACC ATGGGAACAG ATTTTAAGTA TCAGTATGCA 
CATTCATGGT TCCGACAGAT GGACAAGCTG ATTCATTATG TCAACATGGA TGGACGTGTT 
AATGCTCTCT ATTCTACACC ATCCATTTAC ACTGATGCAA AACATGCTAC TAATGAGCAC 
TGGCCAGTAA AGACCGGGGA CTTTTTCCCG TATGCAGATC GTGCAAATGG TTACTGGACT 
GGATACTTTG CAAGTAGACC TGCCTTGAAA CGCTATGTCA GGATGATGAG TGGCTACTAC 
TTGGCTGCAA GACAATTAGA GTTTTACAAT GGGAGGAGCA ATAGAGGACC GAACACAGAC 
TCATTGGCAG ATTCTCTTGC CATTGCTCAG CATCATGACG CTGTTACTGG TACAGAAAAG 
CAACATGTGG CTAATGATTA TGCAAAACGA TTGTCAATTG GCTACACAGA GGCTGAAAAG 
TTAGTTGCAT CCTCACTTGC TTGCTTGGTG GAGTCTGCAT CACATACTGG ATGTCAGAGG 
TCAACTACTA AATTTCAACA GTGCCCACTT CTGAATATTA GTTACTGTCC TGCATCAGAA 
GTAGATCTAT CTCAAGGGAG AAACTTGATT GTTGTTGTCT ACAATGCACT TGGATGGGCG 
AGGGATGATG TAATTCAATT TCCTGTCTTC AATGAAAATG TTATTGTGCA TGATTCTGAG 
AAAAGAGAAA TCGTGTCACA AATTATTCCA ATAGCAGATG CCTTTGTGGG CTTGAGGAAC 
TCACATGTTA ATGCATACTT GGGTAGATCC CCAGTGGAGA CGCCTAAGTA TTGGCTTGCG 
TTTCCAGTTT CTGTTCCACC TTTTGGTTTC AGCACTTATT CCATCTCAAG TGCCAAAAGG 
GCAGGTGCTC ATTCGAGTAA ATCATCTGTA TACACATTAA GAAGTGAGAA ATCTGCTGTT 
GAAGTTGGCC AAGGAAATTT GAAGCTTACA TTTTCTGCAG ATAAAATCAA GCATGCTAAT 
TATGTTAACA GCAGAAGCTC AGTTAAGGAA TCAGTTGAAC AGACATTCAG TTTTTATGCT 
GGATACAATG GAACTGGAAA TGATAAAGAT CCACAGAATT CTGGGGCATA TATCTTCCGT 
CCAAATGGAA CATTTCCTAT AAACCCTGAA TCCCAGGTTC CTTTGACTGT TATGCGTGGA 
CCTGTTTTAG ATGAAGTACA TCAACAGGTC AGCCAATGGA TATATCAGAT CACCAGATTA 
TACAAGGGGA GAGAGCATGT TGAAGTTGAG TTTATCGTTG GGCCCATACC CATTGAAGAT 
GGAATTGGAA AAGAAGTTGC AACCCAAATC ACAACAACCA TGGAAACCAA TAAAACATTT 
TACACAGATT CTAATGGACG TGATTTTATC AAAAGGATTC GTGATTATAG AGCAGATTGG 
GACCTGGAAG TGAATCAACC ATTTGCTGGA AATTATTACC CAATTAATCT TGGAATTTAT 
TTCCAAGATG ACAAGAAGGA ATTTTCAGTT CTGGTGGATC GAGCACTTGG AGGATCTAGT 
CTGGTGGATG GACAAATAGA GCTGATGCTC CATAGGAGAC TGCTACTGGA TGATTCCAGG 
GGTGTTGCGG AGGCTCTTAA TGAAACAGTA TGTGTCCTTG ATCAATGCAA GGGATTGACG 
ATTCAAGGAA AATATTATTA CAGAATTGAC CCTATAGGAG AAGGTGCTAA ATGGCGTCGC 
TCATTCGGCC AGGAGATATA TTCACCCCTC CTCCTTGCGT TTTCTGAAGA GGATGGAGAT 
AATTGGATGA ATTCTCATGT GACCACATTT TCTGGCTTTG ATTCTTCCTA CATTTTGCCT 
GATAATGTGG CAGTTTTAAC TCTCCAGGAG CTTGATGATG GAAAAGTTCT TCTCCGTTTA 
GCTCATTTAT ATGAGATGGG AGAGGACAAG GATCTTTCAG TGATGACAAG CGTAGAACTT 
AGAAAGTTGT TCCCGAAAAA GAAGATCGGC AAAGCTGCAG AAATGAGCTT ATCTGCCAAT 
CAAGAAAGGG CTGAAATGGA GAAAAAGAGG CTTGTGTGGA AAGCAGAAGG ATCATCAAGA 
AAACAAGCAG TGCTGAGGGG TGGACCTGTT GATCCTGCAA AACTGGTGGT GGAACTTGCT 
CCGATGGAAA TCCGCACATT TGTTATTGAC TTCGATCACC AGTTCCACCA TGTTTTTGCT 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7458009 ( XM_002321039.2 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_002321075.2 Populus trichocarpa glycosyl hydrolase family 38 family protein (POPTR_0014s14050g) mRNA, complete cds.

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CloneID	OPa29328
Clone ID Related Accession (Same CDS sequence)	XM_002321039.2
Accession Version	XM_002321039.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-12-30
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	glycosyl hydrolase family 38 family protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008480). On Dec 16, 2013 this sequence version replaced XM_002321039.1.

ATGATGAAAG TGTTGATGGT GGTTTTGTTG CTGTTGCTGC TGGGTGGCTC ATTATTTGTG 
GTGGAAGCCA AGTACATGGT TTATAACACT TCACAAGGTA TAGTGAAAGA TAAGATCAAT 
GTTCACTTGG TCGCTCACAC CCATGATGAT GTTGGTTGGT TGAAGACTGT TGATCAGTAC 
TATGTTGGTT CTAACAATTC TATCCAAGGT GCTTGTGTGC AAAATGTATT GGATTCTCTT 
ATTCCTGCGC TGTTGGCTGA CAAGAACCGG AAATTCATTT ATGCTTTTTT CCAACGGTGG 
TGGAGAGATC AGAGCGAGAC TATGCAGCAT GTAGTCAAGC AACTTGTTAG TTCCGGTCAA 
CTTGAATTCA TAAATGGGGG GATGTGCATG CATGATGAAG CGGTGACACA TTATATTGAC 
ATGATTGACC AGACAACTCT TGGGCATCGG TTTATCAAAA AGGATTTTGG TGTGACTCCA 
AGAGTTGGTT GGCAAATTGA TCCTTTTGGT CATTCTGCGG TGCAGGCTTA CATGTTGGGA 
GCGGAAATTG GATTCGACTC TCTTTTCTTT GGCCGGATAG ATTATCAAGA CCGGGCCAAG 
CGTAAAAATG AGAAAAGTCT TGAAGTTGTC TGGCAGGCTT CCAAGAGTTT TGGTTCGTCT 
GCACAGATTT TTGCTGGTGC TTTCCCAGAA AACTACGAGC CTCCTCCTGG TGGTTTCTAT 
TTTGAAGTCA ATGACCCTTC ACCTGTTGTT CAAGATGACA TCAATTTGTT TGATTACAAT 
GTTCAAGAAC GAGTCGATGA CTTCGTTGCT GCTGCAGTAT CACAGGCTAA TATAACACGA 
ACAAATCATA TAATGTGGAC CATGGGAACA GATTTTAAGT ATCAGTATGC ACATTCATGG 
TTCCGACAGA TGGACAAGCT GATTCATTAT GTCAACATGG ATGGACGTGT TAATGCTCTC 
TATTCTACAC CATCCATTTA CACTGATGCA AAACATGCTA CAAATGAGCA CTGGCCAGTA 
AAGACCGGGG ACTTTTTCCC GTATGCAGAT CGTGCAAATG GTTACTGGAC TGGATACTTT 
GCAAGTAGAC CTGCCTTGAA ACGCTATGTC AGGATGATGA GTGGCTACTA CTTGGCTGCA 
AGACAATTAG AGTTTTACAA TGGGAGGAGC AATAGAGGAC CGAACACAGA CTCATTGGCA 
GATTCTCTTG CCATTGCTCA GCATCATGAC GCTGTTACTG GTACAGAAAA GCAACATGTG 
GCTAATGATT ATGCAAAACG ATTGTCAATT GGCTACACAG AGGCTGAAAA GTTAGTTGCA 
TCCTCACTTG CTTGCTTGGT GGAGTCTGCA TCACATACTG GATGTCAGAG GTCAACTACT 
AAATTTCAAC AGGCAAAAAC TCAAGTAGAT CTATCTCAAG GGAGAAACTT GATTGTTGTT 
GTCTACAATG CACTTGGATG GGCGAGGGAT GATGTAATTC AATTTCCTGT CTTCAATGAA 
AATGTTATTG TGCATGATTC TGAGAAAAGA GAAATCGTGT CACAAATTAT TCCAATAGCA 
GATGCCTTTG TGGGCTTGAG GAACTCACAT GTTAATGCAT ACTTGGGTAG ATCCCCAGTG 
GGGACGCCTA AGTATTGGCT TGCGTTTACA GTTTCTGTTC CACCTTTTGG TTTCAGCACT 
TATTCCATCT CAAGTGCCAA AAGGGCAGGT GCTCATTCGA GTAAATCATC TGTATACACA 
TTAAGAAGTG AGAAATCTGC TGTTGAAGTT GGCCAAGGAA ATTTGAAGCT TACATTTTCT 
GCAGATAAAA TCAAGCATGC TAATTATGTT AACAGCAGAA GCTCAGTTAA GGAATCAGTT 
GAACAGACAT TCAGTTTTTA TGCTGGATAC AATGGAACTG GAAATGATAA AGATCCACAG 
AATTCTGGGG CATATATCTT CCGTCCAAAT GGAACATTTC CTATAAACCC TGAATCCCAG 
GTTCCTTTGA CTGTTATGCG TGGACCTGTT TTAGATGAAG TACATCAACA GGTCAGCCAA 
TGGATATATC AGATCACCAG ATTATACAAG GGGAGAGAGC ATGTTGAAGT TGAGTTTATC 
GTTGGGCCCA TACCCATTGA AGATGGAATT GGAAAAGAAG TTGCAACCCA AATCACAACA 
ACCATGGAAA CCAATAAAAC ATTTTACACA GATTCTAATG GACGTGATTT TATCAAAAGG 
ATTCGTGATT ATAGAGCAGA TTGGGACCTG GAAGTGAATC AACCATTTGC TGGAAATTAT 
TACCCAATTA ATCTTGGAAT TTATTTCCAA GATGACAAGA AGGAATTTTC AGTTCTGGTG 
GATCGAGCAC TTGGAGGATC TAGTCTGGTG GATGGACAAA TAGAGCTGAT GCTCCATAGG 
AGACTGCTAC TGGATGATTC CAGGGGTGTT GCGGAGGCTC TTAATGAAAC AGTATGTGTC 
CTTGATCAAT GCAAGGGATT GACGATTCAA GGAAAATATT ATTACAGAAT TGACCCTATA 
GGAGAAGGTG CTAAATGGCG TCGCTCATTC GGCCAGGAGA TATATTCACC CCTCCTCCTT 
GCGTTTTCTG AAGAGGATGG AGATAATTGG ATGAATTCTC ATGTGACCAC ATTTTCTGGC 
TTTGATTCTT CCTACATTTT GCCTGATAAT GTGGCAGTTT TAACTCTCCA GGAGCTTGAT 
GATGGAAAAG TTCTTCTCCG TTTAGCTCAT TTATATGAGA TGGGAGAGGA CAAGGATCTT 
TCAGTGATGA CAAGCGTAGA ACTTAGAAAG TTGTTCCCGA AAAAGAAGAT CGGCAAAGCT 
GCAGAAATGA GCTTATCTGC CAATCAAGAA AGGGCTGAAA TGGAGAAAAA GAGGCTTGTG 
TGGAAAGCAG AAGGATCATC AAGAAAACAA GCAGTGCTGA GGGGTGGACC TGTTGATCCT 
GCAAAACTGG TGGTGGAACT TGCTCCGATG GAAATCCGCA CATTTGTTAT TGACTTCGAT 
CACCAGTTCC ACCATGTTTT TGCTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002321075.2
CDS	282..3311
Translation	MMKVLMVVLLLLLLGGSLFVVEAKYMVYNTSQGIVKDKINVHLVAHTHDDVGWLKTVDQYYVGSNNSIQGACVQNVLDSLIPALLADKNRKFIYAFFQRWWRDQSETMQHVVKQLVSSGQLEFINGGMCMHDEAVTHYIDMIDQTTLGHRFIKKDFGVTPRVGWQIDPFGHSAVQAYMLGAEIGFDSLFFGRIDYQDRAKRKNEKSLEVVWQASKSFGSSAQIFAGAFPENYEPPPGGFYFEVNDPSPVVQDDINLFDYNVQERVDDFVAAAVSQANITRTNHIMWTMGTDFKYQYAHSWFRQMDKLIHYVNMDGRVNALYSTPSIYTDAKHATNEHWPVKTGDFFPYADRANGYWTGYFASRPALKRYVRMMSGYYLAARQLEFYNGRSNRGPNTDSLADSLAIAQHHDAVTGTEKQHVANDYAKRLSIGYTEAEKLVASSLACLVESASHTGCQRSTTKFQQAKTQVDLSQGRNLIVVVYNALGWARDDVIQFPVFNENVIVHDSEKREIVSQIIPIADAFVGLRNSHVNAYLGRSPVGTPKYWLAFTVSVPPFGFSTYSISSAKRAGAHSSKSSVYTLRSEKSAVEVGQGNLKLTFSADKIKHANYVNSRSSVKESVEQTFSFYAGYNGTGNDKDPQNSGAYIFRPNGTFPINPESQVPLTVMRGPVLDEVHQQVSQWIYQITRLYKGREHVEVEFIVGPIPIEDGIGKEVATQITTTMETNKTFYTDSNGRDFIKRIRDYRADWDLEVNQPFAGNYYPINLGIYFQDDKKEFSVLVDRALGGSSLVDGQIELMLHRRLLLDDSRGVAEALNETVCVLDQCKGLTIQGKYYYRIDPIGEGAKWRRSFGQEIYSPLLLAFSEEDGDNWMNSHVTTFSGFDSSYILPDNVAVLTLQELDDGKVLLRLAHLYEMGEDKDLSVMTSVELRKLFPKKKIGKAAEMSLSANQERAEMEKKRLVWKAEGSSRKQAVLRGGPVDPAKLVVELAPMEIRTFVIDFDHQFHHVFAA

Target ORF information:

RefSeq Version	XM_002321039.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa glycosyl hydrolase family 38 family protein (POPTR_0014s14050g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002321039.2

ORF Insert Sequence:

ATGATGAAAG TGTTGATGGT GGTTTTGTTG CTGTTGCTGC TGGGTGGCTC ATTATTTGTG 
GTGGAAGCCA AGTACATGGT TTATAACACT TCACAAGGTA TAGTGAAAGA TAAGATCAAT 
GTTCACTTGG TCGCTCACAC CCATGATGAT GTTGGTTGGT TGAAGACTGT TGATCAGTAC 
TATGTTGGTT CTAACAATTC TATCCAAGGT GCTTGTGTGC AAAATGTATT GGATTCTCTT 
ATTCCTGCGC TGTTGGCTGA CAAGAACCGG AAATTCATTT ATGCTTTTTT CCAACGGTGG 
TGGAGAGATC AGAGCGAGAC TATGCAGCAT GTAGTCAAGC AACTTGTTAG TTCCGGTCAA 
CTTGAATTCA TAAATGGGGG GATGTGCATG CATGATGAAG CGGTGACACA TTATATTGAC 
ATGATTGACC AGACAACTCT TGGGCATCGG TTTATCAAAA AGGATTTTGG TGTGACTCCA 
AGAGTTGGTT GGCAAATTGA TCCTTTTGGT CATTCTGCGG TGCAGGCTTA CATGTTGGGA 
GCGGAAATTG GATTCGACTC TCTTTTCTTT GGCCGGATAG ATTATCAAGA CCGGGCCAAG 
CGTAAAAATG AGAAAAGTCT TGAAGTTGTC TGGCAGGCTT CCAAGAGTTT TGGTTCGTCT 
GCACAGATTT TTGCTGGTGC TTTCCCAGAA AACTACGAGC CTCCTCCTGG TGGTTTCTAT 
TTTGAAGTCA ATGACCCTTC ACCTGTTGTT CAAGATGACA TCAATTTGTT TGATTACAAT 
GTTCAAGAAC GAGTCGATGA CTTCGTTGCT GCTGCAGTAT CACAGGCTAA TATAACACGA 
ACAAATCATA TAATGTGGAC CATGGGAACA GATTTTAAGT ATCAGTATGC ACATTCATGG 
TTCCGACAGA TGGACAAGCT GATTCATTAT GTCAACATGG ATGGACGTGT TAATGCTCTC 
TATTCTACAC CATCCATTTA CACTGATGCA AAACATGCTA CAAATGAGCA CTGGCCAGTA 
AAGACCGGGG ACTTTTTCCC GTATGCAGAT CGTGCAAATG GTTACTGGAC TGGATACTTT 
GCAAGTAGAC CTGCCTTGAA ACGCTATGTC AGGATGATGA GTGGCTACTA CTTGGCTGCA 
AGACAATTAG AGTTTTACAA TGGGAGGAGC AATAGAGGAC CGAACACAGA CTCATTGGCA 
GATTCTCTTG CCATTGCTCA GCATCATGAC GCTGTTACTG GTACAGAAAA GCAACATGTG 
GCTAATGATT ATGCAAAACG ATTGTCAATT GGCTACACAG AGGCTGAAAA GTTAGTTGCA 
TCCTCACTTG CTTGCTTGGT GGAGTCTGCA TCACATACTG GATGTCAGAG GTCAACTACT 
AAATTTCAAC AGGCAAAAAC TCAAGTAGAT CTATCTCAAG GGAGAAACTT GATTGTTGTT 
GTCTACAATG CACTTGGATG GGCGAGGGAT GATGTAATTC AATTTCCTGT CTTCAATGAA 
AATGTTATTG TGCATGATTC TGAGAAAAGA GAAATCGTGT CACAAATTAT TCCAATAGCA 
GATGCCTTTG TGGGCTTGAG GAACTCACAT GTTAATGCAT ACTTGGGTAG ATCCCCAGTG 
GGGACGCCTA AGTATTGGCT TGCGTTTACA GTTTCTGTTC CACCTTTTGG TTTCAGCACT 
TATTCCATCT CAAGTGCCAA AAGGGCAGGT GCTCATTCGA GTAAATCATC TGTATACACA 
TTAAGAAGTG AGAAATCTGC TGTTGAAGTT GGCCAAGGAA ATTTGAAGCT TACATTTTCT 
GCAGATAAAA TCAAGCATGC TAATTATGTT AACAGCAGAA GCTCAGTTAA GGAATCAGTT 
GAACAGACAT TCAGTTTTTA TGCTGGATAC AATGGAACTG GAAATGATAA AGATCCACAG 
AATTCTGGGG CATATATCTT CCGTCCAAAT GGAACATTTC CTATAAACCC TGAATCCCAG 
GTTCCTTTGA CTGTTATGCG TGGACCTGTT TTAGATGAAG TACATCAACA GGTCAGCCAA 
TGGATATATC AGATCACCAG ATTATACAAG GGGAGAGAGC ATGTTGAAGT TGAGTTTATC 
GTTGGGCCCA TACCCATTGA AGATGGAATT GGAAAAGAAG TTGCAACCCA AATCACAACA 
ACCATGGAAA CCAATAAAAC ATTTTACACA GATTCTAATG GACGTGATTT TATCAAAAGG 
ATTCGTGATT ATAGAGCAGA TTGGGACCTG GAAGTGAATC AACCATTTGC TGGAAATTAT 
TACCCAATTA ATCTTGGAAT TTATTTCCAA GATGACAAGA AGGAATTTTC AGTTCTGGTG 
GATCGAGCAC TTGGAGGATC TAGTCTGGTG GATGGACAAA TAGAGCTGAT GCTCCATAGG 
AGACTGCTAC TGGATGATTC CAGGGGTGTT GCGGAGGCTC TTAATGAAAC AGTATGTGTC 
CTTGATCAAT GCAAGGGATT GACGATTCAA GGAAAATATT ATTACAGAAT TGACCCTATA 
GGAGAAGGTG CTAAATGGCG TCGCTCATTC GGCCAGGAGA TATATTCACC CCTCCTCCTT 
GCGTTTTCTG AAGAGGATGG AGATAATTGG ATGAATTCTC ATGTGACCAC ATTTTCTGGC 
TTTGATTCTT CCTACATTTT GCCTGATAAT GTGGCAGTTT TAACTCTCCA GGAGCTTGAT 
GATGGAAAAG TTCTTCTCCG TTTAGCTCAT TTATATGAGA TGGGAGAGGA CAAGGATCTT 
TCAGTGATGA CAAGCGTAGA ACTTAGAAAG TTGTTCCCGA AAAAGAAGAT CGGCAAAGCT 
GCAGAAATGA GCTTATCTGC CAATCAAGAA AGGGCTGAAA TGGAGAAAAA GAGGCTTGTG 
TGGAAAGCAG AAGGATCATC AAGAAAACAA GCAGTGCTGA GGGGTGGACC TGTTGATCCT 
GCAAAACTGG TGGTGGAACT TGCTCCGATG GAAATCCGCA CATTTGTTAT TGACTTCGAT 
CACCAGTTCC ACCATGTTTT TGCTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,D?jardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasj?rvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Lepl? JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouz? P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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LOC7458009 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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電話

FAX

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