LOC105114327 cDNA ORF clone, Populus euphratica(Euphrates poplar)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105114327
Entrez Gene ID	105114327
Full Name	neutral ceramidase
Gene Type	protein-coding
Organism	Populus euphratica(Euphrates poplar)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011010846.1	XP_011009148.1	neutral ceramidase
XM_011010845.1	XP_011009147.1	neutral ceramidase

Pathways from BioSystems
Gene Ontology

The following LOC105114327 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105114327 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo294726	XM_011010846.1 Latest version!	Populus euphratica neutral ceramidase (LOC105114327), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPo294726	XM_011010845.1 Latest version!	Populus euphratica neutral ceramidase (LOC105114327), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105114327 ( XM_011010846.1 ) cDNA ORF clone, Populus euphratica(Euphrates poplar) -> XP_011009148.1 Populus euphratica neutral ceramidase (LOC105114327), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo294726
Clone ID Related Accession (Same CDS sequence)	XM_011010846.1 , XM_011010845.1
Accession Version	XM_011010846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-05
Organism	Populus euphratica(Euphrates poplar)
Product	neutral ceramidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011500390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Populus euphratica Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATTT TTCCGAGAGG GTATAGTGGG TTATGGGTGC AAATTGCATC CGTTTTGTTC 
TTGGTGATGT TAATGCAGAA TTTTAGAGGG AGTTTATCAA CTTCCAATTA CTTGATTGGA 
CTCGGGAGCT ATGACATAAC AGGGCCTGCA GCTGATGTCA ACATGATGGG GTATGCAAAT 
ACGGAACAGA TTGCTTCTGG TGTTCACTTC AGGTTGCGAG CTCGTGCATT CATTGTGGCT 
GAACCTCAGG GGAGCCGTGT TGTATATGTG AACCTTGATG CTTGTATGGC TTCGCAGATT 
GTTACTATTA AAGTTCTTGA GAGATTGAAA GCAAGGTATG GAGGGCTTTA TACAGAGCAG 
AATGTTGCGA TTAGTGGTAT CCACACCCAT GCCGGGCCTG GAGGCTATCT GCAATATGTT 
GTATATATTG TGACTTCGCT TGGGTTTGTT CGCCAGTCTT TTGATGTTAT TGTTGATGGT 
ATTGAGAAAA GCATCATACA AGCTCATGAA AATCTTCGGC CAGGGTCAAT TTTTGTAAAT 
AAAGGAGAGC TGTTAGATGC TGGTGTAAAT CGCAGTCCTA GTGCTTATCT TAATAATCCT 
GCAGAAGAGC GGAGTAAATA TAAGTATGAT GTGGATAAAG AAATGACCCT AATAAAGTTT 
GTGGATGATG AGTGGGGGCC AGTAGGTAGT TTTAACTGGT TTGCAACTCA TGGAACTTCA 
ATGAGTCGTA CAAACTCACT GATAAGTGGT GACAACAAAG GAGCTGCTGC ACGATTTATG 
GAGGACTGGT TTGAGAAGAA AGGCCATGTT GAAAATTTGG ATAGCCAACA TGCTAATAAA 
TCTGGAACTG CAAAAATCCC TCGAAGAGTC TCAGGCATAG TTCCAAGCAT CAATGAGAAC 
CGTAAGGAAG CGATGGAAGT TGCCGCCTCC TTCAAATCTT CTCAGGGGCA ACCTGCTACG 
AGGTATTCAA GTGTTGCAAA ACGGGTCAGA AATTCTTTGA GGCTGGCTGA CAGACCCCAT 
TATGTATCTG CGTTCTGTCA AACTAATTGC GGTGATGTAA GTCCCAATGT TCTTGGTGCA 
TTCTGCATTG ACACTGGACT ACCTTGTGAT TTCAATCATA GTACCTGCAA CGGGAAGAAT 
GAACAGTGTT ATGGCAGGGG CCCAGGTTAT CCAGATGAAT TTGAGAGTAC AAGAATCATT 
GGAGAAAGAC AATTCAAAAA AGCTGTCGAA TTGTTCAACA AAGCAACTGA GCAGCTGAAA 
GGGAAGGTTG GGTACCGACA TGCTTATGTG AATTTCTCAA ATCTTGAAGT TGCACAAGGC 
AATGACGTGG TGAAGACATG TCCTGCAGCC ATGGGCTTTG CTTTTGCTGC TGGAACCACC 
GATGGACCTG GAGCTTTTGA TTTCAAGCAA GGAGATGACA AGGGCAATGC CTTCTGGAGG 
TTGGTGAGGG ACTTTCTGAA AACACCAAAT CAGGAACAAG TCAATTGTCA GCGTCCAAAG 
CCTATTCTGC TTGACACTGG TGAAATGGAC AAACCGTATG CCTGGGCACC TTCAATACTT 
CCAGTTCAAA TCTTGCGGAT AGGACAACTT GTCATTCTCA GTGTACCTGG AGAGTTCACT 
ACCATGGCTG GCAGGCGCCT TCGCGATGCT GTGAAGATGG TTCTCACTTC AGGAGCTAGC 
AAAGAGTTCG GGAGAAATGT TCACATTGTC ATTTCTGGTC TGACAAATAC ATATTCACAG 
TATGTGACAA CCTTTGAGGA GTATGAAGTG CAGAGATATG AGGGGGCCTC CACTCTTTAT 
GGCCCACACA CACTCAGTGC CTACATTCAG GAGTTCAGGA AACTAGCTGC AGCTCTCATC 
AGTGGCCGAC CTGTGGAACC AGGTCCACAA CCCCCAGATC TCCTTGACAA GCAAATCAGT 
TTATTAACTC CAGTTGTCTT AGATTCAACC CGTCCTGGTG CAAAGTTTGG AGATGTTAAG 
AGTGACGTCC CTTTGAACTC CACCTTCAAA AGGGGTGACA TGGTTACAGT CACATTCTGG 
TCTGCTTGCC CAAGGAATGA CCTCCTGACC GAGGGCACAT TTGCTCTAGT TGAGATTCTC 
CAGGGCCAGA AGACATGGAT TCCAGCATAC GACGATGACG ACTTCTGCCT GCGGTTTATT 
TGGTCACGGC CTTCAAAACT TAGTCCTCAG AGTTACGCAA CCATAGAATG GAGAATTCCG 
CAGTCAGCAG TCTCTGGTGT GTACAGGATA CGACATTTTG GTGCTGCGAA GGCACTCTTT 
GGTTCAATCA GCCATTTCAC AGGTGCTTCG AGTGCGTTTA TAGTAGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011009148.1
CDS	96..2426
Translation	MDIFPRGYSGLWVQIASVLFLVMLMQNFRGSLSTSNYLIGLGSYDITGPAADVNMMGYANTEQIASGVHFRLRARAFIVAEPQGSRVVYVNLDACMASQIVTIKVLERLKARYGGLYTEQNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVIVDGIEKSIIQAHENLRPGSIFVNKGELLDAGVNRSPSAYLNNPAEERSKYKYDVDKEMTLIKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEKKGHVENLDSQHANKSGTAKIPRRVSGIVPSINENRKEAMEVAASFKSSQGQPATRYSSVAKRVRNSLRLADRPHYVSAFCQTNCGDVSPNVLGAFCIDTGLPCDFNHSTCNGKNEQCYGRGPGYPDEFESTRIIGERQFKKAVELFNKATEQLKGKVGYRHAYVNFSNLEVAQGNDVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWRLVRDFLKTPNQEQVNCQRPKPILLDTGEMDKPYAWAPSILPVQILRIGQLVILSVPGEFTTMAGRRLRDAVKMVLTSGASKEFGRNVHIVISGLTNTYSQYVTTFEEYEVQRYEGASTLYGPHTLSAYIQEFRKLAAALISGRPVEPGPQPPDLLDKQISLLTPVVLDSTRPGAKFGDVKSDVPLNSTFKRGDMVTVTFWSACPRNDLLTEGTFALVEILQGQKTWIPAYDDDDFCLRFIWSRPSKLSPQSYATIEWRIPQSAVSGVYRIRHFGAAKALFGSISHFTGASSAFIVA

Target ORF information:

RefSeq Version	XM_011010846.1
Organism	Populus euphratica(Euphrates poplar)
Definition	Populus euphratica neutral ceramidase (LOC105114327), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011010846.1

ORF Insert Sequence:

ATGGACATTT TTCCGAGAGG GTATAGTGGG TTATGGGTGC AAATTGCATC CGTTTTGTTC 
TTGGTGATGT TAATGCAGAA TTTTAGAGGG AGTTTATCAA CTTCCAATTA CTTGATTGGA 
CTCGGGAGCT ATGACATAAC AGGGCCTGCA GCTGATGTCA ACATGATGGG GTATGCAAAT 
ACGGAACAGA TTGCTTCTGG TGTTCACTTC AGGTTGCGAG CTCGTGCATT CATTGTGGCT 
GAACCTCAGG GGAGCCGTGT TGTATATGTG AACCTTGATG CTTGTATGGC TTCGCAGATT 
GTTACTATTA AAGTTCTTGA GAGATTGAAA GCAAGGTATG GAGGGCTTTA TACAGAGCAG 
AATGTTGCGA TTAGTGGTAT CCACACCCAT GCCGGGCCTG GAGGCTATCT GCAATATGTT 
GTATATATTG TGACTTCGCT TGGGTTTGTT CGCCAGTCTT TTGATGTTAT TGTTGATGGT 
ATTGAGAAAA GCATCATACA AGCTCATGAA AATCTTCGGC CAGGGTCAAT TTTTGTAAAT 
AAAGGAGAGC TGTTAGATGC TGGTGTAAAT CGCAGTCCTA GTGCTTATCT TAATAATCCT 
GCAGAAGAGC GGAGTAAATA TAAGTATGAT GTGGATAAAG AAATGACCCT AATAAAGTTT 
GTGGATGATG AGTGGGGGCC AGTAGGTAGT TTTAACTGGT TTGCAACTCA TGGAACTTCA 
ATGAGTCGTA CAAACTCACT GATAAGTGGT GACAACAAAG GAGCTGCTGC ACGATTTATG 
GAGGACTGGT TTGAGAAGAA AGGCCATGTT GAAAATTTGG ATAGCCAACA TGCTAATAAA 
TCTGGAACTG CAAAAATCCC TCGAAGAGTC TCAGGCATAG TTCCAAGCAT CAATGAGAAC 
CGTAAGGAAG CGATGGAAGT TGCCGCCTCC TTCAAATCTT CTCAGGGGCA ACCTGCTACG 
AGGTATTCAA GTGTTGCAAA ACGGGTCAGA AATTCTTTGA GGCTGGCTGA CAGACCCCAT 
TATGTATCTG CGTTCTGTCA AACTAATTGC GGTGATGTAA GTCCCAATGT TCTTGGTGCA 
TTCTGCATTG ACACTGGACT ACCTTGTGAT TTCAATCATA GTACCTGCAA CGGGAAGAAT 
GAACAGTGTT ATGGCAGGGG CCCAGGTTAT CCAGATGAAT TTGAGAGTAC AAGAATCATT 
GGAGAAAGAC AATTCAAAAA AGCTGTCGAA TTGTTCAACA AAGCAACTGA GCAGCTGAAA 
GGGAAGGTTG GGTACCGACA TGCTTATGTG AATTTCTCAA ATCTTGAAGT TGCACAAGGC 
AATGACGTGG TGAAGACATG TCCTGCAGCC ATGGGCTTTG CTTTTGCTGC TGGAACCACC 
GATGGACCTG GAGCTTTTGA TTTCAAGCAA GGAGATGACA AGGGCAATGC CTTCTGGAGG 
TTGGTGAGGG ACTTTCTGAA AACACCAAAT CAGGAACAAG TCAATTGTCA GCGTCCAAAG 
CCTATTCTGC TTGACACTGG TGAAATGGAC AAACCGTATG CCTGGGCACC TTCAATACTT 
CCAGTTCAAA TCTTGCGGAT AGGACAACTT GTCATTCTCA GTGTACCTGG AGAGTTCACT 
ACCATGGCTG GCAGGCGCCT TCGCGATGCT GTGAAGATGG TTCTCACTTC AGGAGCTAGC 
AAAGAGTTCG GGAGAAATGT TCACATTGTC ATTTCTGGTC TGACAAATAC ATATTCACAG 
TATGTGACAA CCTTTGAGGA GTATGAAGTG CAGAGATATG AGGGGGCCTC CACTCTTTAT 
GGCCCACACA CACTCAGTGC CTACATTCAG GAGTTCAGGA AACTAGCTGC AGCTCTCATC 
AGTGGCCGAC CTGTGGAACC AGGTCCACAA CCCCCAGATC TCCTTGACAA GCAAATCAGT 
TTATTAACTC CAGTTGTCTT AGATTCAACC CGTCCTGGTG CAAAGTTTGG AGATGTTAAG 
AGTGACGTCC CTTTGAACTC CACCTTCAAA AGGGGTGACA TGGTTACAGT CACATTCTGG 
TCTGCTTGCC CAAGGAATGA CCTCCTGACC GAGGGCACAT TTGCTCTAGT TGAGATTCTC 
CAGGGCCAGA AGACATGGAT TCCAGCATAC GACGATGACG ACTTCTGCCT GCGGTTTATT 
TGGTCACGGC CTTCAAAACT TAGTCCTCAG AGTTACGCAA CCATAGAATG GAGAATTCCG 
CAGTCAGCAG TCTCTGGTGT GTACAGGATA CGACATTTTG GTGCTGCGAA GGCACTCTTT 
GGTTCAATCA GCCATTTCAC AGGTGCTTCG AGTGCGTTTA TAGTAGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105114327 ( XM_011010845.1 ) cDNA ORF clone, Populus euphratica(Euphrates poplar) -> XP_011009147.1 Populus euphratica neutral ceramidase (LOC105114327), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo294726
Clone ID Related Accession (Same CDS sequence)	XM_011010846.1 , XM_011010845.1
Accession Version	XM_011010845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-05
Organism	Populus euphratica(Euphrates poplar)
Product	neutral ceramidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011500390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Populus euphratica Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATTT TTCCGAGAGG GTATAGTGGG TTATGGGTGC AAATTGCATC CGTTTTGTTC 
TTGGTGATGT TAATGCAGAA TTTTAGAGGG AGTTTATCAA CTTCCAATTA CTTGATTGGA 
CTCGGGAGCT ATGACATAAC AGGGCCTGCA GCTGATGTCA ACATGATGGG GTATGCAAAT 
ACGGAACAGA TTGCTTCTGG TGTTCACTTC AGGTTGCGAG CTCGTGCATT CATTGTGGCT 
GAACCTCAGG GGAGCCGTGT TGTATATGTG AACCTTGATG CTTGTATGGC TTCGCAGATT 
GTTACTATTA AAGTTCTTGA GAGATTGAAA GCAAGGTATG GAGGGCTTTA TACAGAGCAG 
AATGTTGCGA TTAGTGGTAT CCACACCCAT GCCGGGCCTG GAGGCTATCT GCAATATGTT 
GTATATATTG TGACTTCGCT TGGGTTTGTT CGCCAGTCTT TTGATGTTAT TGTTGATGGT 
ATTGAGAAAA GCATCATACA AGCTCATGAA AATCTTCGGC CAGGGTCAAT TTTTGTAAAT 
AAAGGAGAGC TGTTAGATGC TGGTGTAAAT CGCAGTCCTA GTGCTTATCT TAATAATCCT 
GCAGAAGAGC GGAGTAAATA TAAGTATGAT GTGGATAAAG AAATGACCCT AATAAAGTTT 
GTGGATGATG AGTGGGGGCC AGTAGGTAGT TTTAACTGGT TTGCAACTCA TGGAACTTCA 
ATGAGTCGTA CAAACTCACT GATAAGTGGT GACAACAAAG GAGCTGCTGC ACGATTTATG 
GAGGACTGGT TTGAGAAGAA AGGCCATGTT GAAAATTTGG ATAGCCAACA TGCTAATAAA 
TCTGGAACTG CAAAAATCCC TCGAAGAGTC TCAGGCATAG TTCCAAGCAT CAATGAGAAC 
CGTAAGGAAG CGATGGAAGT TGCCGCCTCC TTCAAATCTT CTCAGGGGCA ACCTGCTACG 
AGGTATTCAA GTGTTGCAAA ACGGGTCAGA AATTCTTTGA GGCTGGCTGA CAGACCCCAT 
TATGTATCTG CGTTCTGTCA AACTAATTGC GGTGATGTAA GTCCCAATGT TCTTGGTGCA 
TTCTGCATTG ACACTGGACT ACCTTGTGAT TTCAATCATA GTACCTGCAA CGGGAAGAAT 
GAACAGTGTT ATGGCAGGGG CCCAGGTTAT CCAGATGAAT TTGAGAGTAC AAGAATCATT 
GGAGAAAGAC AATTCAAAAA AGCTGTCGAA TTGTTCAACA AAGCAACTGA GCAGCTGAAA 
GGGAAGGTTG GGTACCGACA TGCTTATGTG AATTTCTCAA ATCTTGAAGT TGCACAAGGC 
AATGACGTGG TGAAGACATG TCCTGCAGCC ATGGGCTTTG CTTTTGCTGC TGGAACCACC 
GATGGACCTG GAGCTTTTGA TTTCAAGCAA GGAGATGACA AGGGCAATGC CTTCTGGAGG 
TTGGTGAGGG ACTTTCTGAA AACACCAAAT CAGGAACAAG TCAATTGTCA GCGTCCAAAG 
CCTATTCTGC TTGACACTGG TGAAATGGAC AAACCGTATG CCTGGGCACC TTCAATACTT 
CCAGTTCAAA TCTTGCGGAT AGGACAACTT GTCATTCTCA GTGTACCTGG AGAGTTCACT 
ACCATGGCTG GCAGGCGCCT TCGCGATGCT GTGAAGATGG TTCTCACTTC AGGAGCTAGC 
AAAGAGTTCG GGAGAAATGT TCACATTGTC ATTTCTGGTC TGACAAATAC ATATTCACAG 
TATGTGACAA CCTTTGAGGA GTATGAAGTG CAGAGATATG AGGGGGCCTC CACTCTTTAT 
GGCCCACACA CACTCAGTGC CTACATTCAG GAGTTCAGGA AACTAGCTGC AGCTCTCATC 
AGTGGCCGAC CTGTGGAACC AGGTCCACAA CCCCCAGATC TCCTTGACAA GCAAATCAGT 
TTATTAACTC CAGTTGTCTT AGATTCAACC CGTCCTGGTG CAAAGTTTGG AGATGTTAAG 
AGTGACGTCC CTTTGAACTC CACCTTCAAA AGGGGTGACA TGGTTACAGT CACATTCTGG 
TCTGCTTGCC CAAGGAATGA CCTCCTGACC GAGGGCACAT TTGCTCTAGT TGAGATTCTC 
CAGGGCCAGA AGACATGGAT TCCAGCATAC GACGATGACG ACTTCTGCCT GCGGTTTATT 
TGGTCACGGC CTTCAAAACT TAGTCCTCAG AGTTACGCAA CCATAGAATG GAGAATTCCG 
CAGTCAGCAG TCTCTGGTGT GTACAGGATA CGACATTTTG GTGCTGCGAA GGCACTCTTT 
GGTTCAATCA GCCATTTCAC AGGTGCTTCG AGTGCGTTTA TAGTAGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011009147.1
CDS	111..2441
Translation	MDIFPRGYSGLWVQIASVLFLVMLMQNFRGSLSTSNYLIGLGSYDITGPAADVNMMGYANTEQIASGVHFRLRARAFIVAEPQGSRVVYVNLDACMASQIVTIKVLERLKARYGGLYTEQNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVIVDGIEKSIIQAHENLRPGSIFVNKGELLDAGVNRSPSAYLNNPAEERSKYKYDVDKEMTLIKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEKKGHVENLDSQHANKSGTAKIPRRVSGIVPSINENRKEAMEVAASFKSSQGQPATRYSSVAKRVRNSLRLADRPHYVSAFCQTNCGDVSPNVLGAFCIDTGLPCDFNHSTCNGKNEQCYGRGPGYPDEFESTRIIGERQFKKAVELFNKATEQLKGKVGYRHAYVNFSNLEVAQGNDVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWRLVRDFLKTPNQEQVNCQRPKPILLDTGEMDKPYAWAPSILPVQILRIGQLVILSVPGEFTTMAGRRLRDAVKMVLTSGASKEFGRNVHIVISGLTNTYSQYVTTFEEYEVQRYEGASTLYGPHTLSAYIQEFRKLAAALISGRPVEPGPQPPDLLDKQISLLTPVVLDSTRPGAKFGDVKSDVPLNSTFKRGDMVTVTFWSACPRNDLLTEGTFALVEILQGQKTWIPAYDDDDFCLRFIWSRPSKLSPQSYATIEWRIPQSAVSGVYRIRHFGAAKALFGSISHFTGASSAFIVA

Target ORF information:

RefSeq Version	XM_011010845.1
Organism	Populus euphratica(Euphrates poplar)
Definition	Populus euphratica neutral ceramidase (LOC105114327), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011010845.1

ORF Insert Sequence:

ATGGACATTT TTCCGAGAGG GTATAGTGGG TTATGGGTGC AAATTGCATC CGTTTTGTTC 
TTGGTGATGT TAATGCAGAA TTTTAGAGGG AGTTTATCAA CTTCCAATTA CTTGATTGGA 
CTCGGGAGCT ATGACATAAC AGGGCCTGCA GCTGATGTCA ACATGATGGG GTATGCAAAT 
ACGGAACAGA TTGCTTCTGG TGTTCACTTC AGGTTGCGAG CTCGTGCATT CATTGTGGCT 
GAACCTCAGG GGAGCCGTGT TGTATATGTG AACCTTGATG CTTGTATGGC TTCGCAGATT 
GTTACTATTA AAGTTCTTGA GAGATTGAAA GCAAGGTATG GAGGGCTTTA TACAGAGCAG 
AATGTTGCGA TTAGTGGTAT CCACACCCAT GCCGGGCCTG GAGGCTATCT GCAATATGTT 
GTATATATTG TGACTTCGCT TGGGTTTGTT CGCCAGTCTT TTGATGTTAT TGTTGATGGT 
ATTGAGAAAA GCATCATACA AGCTCATGAA AATCTTCGGC CAGGGTCAAT TTTTGTAAAT 
AAAGGAGAGC TGTTAGATGC TGGTGTAAAT CGCAGTCCTA GTGCTTATCT TAATAATCCT 
GCAGAAGAGC GGAGTAAATA TAAGTATGAT GTGGATAAAG AAATGACCCT AATAAAGTTT 
GTGGATGATG AGTGGGGGCC AGTAGGTAGT TTTAACTGGT TTGCAACTCA TGGAACTTCA 
ATGAGTCGTA CAAACTCACT GATAAGTGGT GACAACAAAG GAGCTGCTGC ACGATTTATG 
GAGGACTGGT TTGAGAAGAA AGGCCATGTT GAAAATTTGG ATAGCCAACA TGCTAATAAA 
TCTGGAACTG CAAAAATCCC TCGAAGAGTC TCAGGCATAG TTCCAAGCAT CAATGAGAAC 
CGTAAGGAAG CGATGGAAGT TGCCGCCTCC TTCAAATCTT CTCAGGGGCA ACCTGCTACG 
AGGTATTCAA GTGTTGCAAA ACGGGTCAGA AATTCTTTGA GGCTGGCTGA CAGACCCCAT 
TATGTATCTG CGTTCTGTCA AACTAATTGC GGTGATGTAA GTCCCAATGT TCTTGGTGCA 
TTCTGCATTG ACACTGGACT ACCTTGTGAT TTCAATCATA GTACCTGCAA CGGGAAGAAT 
GAACAGTGTT ATGGCAGGGG CCCAGGTTAT CCAGATGAAT TTGAGAGTAC AAGAATCATT 
GGAGAAAGAC AATTCAAAAA AGCTGTCGAA TTGTTCAACA AAGCAACTGA GCAGCTGAAA 
GGGAAGGTTG GGTACCGACA TGCTTATGTG AATTTCTCAA ATCTTGAAGT TGCACAAGGC 
AATGACGTGG TGAAGACATG TCCTGCAGCC ATGGGCTTTG CTTTTGCTGC TGGAACCACC 
GATGGACCTG GAGCTTTTGA TTTCAAGCAA GGAGATGACA AGGGCAATGC CTTCTGGAGG 
TTGGTGAGGG ACTTTCTGAA AACACCAAAT CAGGAACAAG TCAATTGTCA GCGTCCAAAG 
CCTATTCTGC TTGACACTGG TGAAATGGAC AAACCGTATG CCTGGGCACC TTCAATACTT 
CCAGTTCAAA TCTTGCGGAT AGGACAACTT GTCATTCTCA GTGTACCTGG AGAGTTCACT 
ACCATGGCTG GCAGGCGCCT TCGCGATGCT GTGAAGATGG TTCTCACTTC AGGAGCTAGC 
AAAGAGTTCG GGAGAAATGT TCACATTGTC ATTTCTGGTC TGACAAATAC ATATTCACAG 
TATGTGACAA CCTTTGAGGA GTATGAAGTG CAGAGATATG AGGGGGCCTC CACTCTTTAT 
GGCCCACACA CACTCAGTGC CTACATTCAG GAGTTCAGGA AACTAGCTGC AGCTCTCATC 
AGTGGCCGAC CTGTGGAACC AGGTCCACAA CCCCCAGATC TCCTTGACAA GCAAATCAGT 
TTATTAACTC CAGTTGTCTT AGATTCAACC CGTCCTGGTG CAAAGTTTGG AGATGTTAAG 
AGTGACGTCC CTTTGAACTC CACCTTCAAA AGGGGTGACA TGGTTACAGT CACATTCTGG 
TCTGCTTGCC CAAGGAATGA CCTCCTGACC GAGGGCACAT TTGCTCTAGT TGAGATTCTC 
CAGGGCCAGA AGACATGGAT TCCAGCATAC GACGATGACG ACTTCTGCCT GCGGTTTATT 
TGGTCACGGC CTTCAAAACT TAGTCCTCAG AGTTACGCAA CCATAGAATG GAGAATTCCG 
CAGTCAGCAG TCTCTGGTGT GTACAGGATA CGACATTTTG GTGCTGCGAA GGCACTCTTT 
GGTTCAATCA GCCATTTCAC AGGTGCTTCG AGTGCGTTTA TAGTAGCATA A