CILP cDNA ORF clone, Pogona vitticeps(central bearded dragon)

Gene
Clones
gRNAs

Summary

Gene Symbol	CILP
Entrez Gene ID	110089257
Full Name	cartilage intermediate layer protein
Gene Type	protein-coding
Organism	Pogona vitticeps(central bearded dragon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020812169.1	XP_020667828.1	cartilage intermediate layer protein 1 isoform X2
XM_020812168.1	XP_020667827.1	cartilage intermediate layer protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following CILP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CILP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo256092	XM_020812168.1 Latest version!	Pogona vitticeps cartilage intermediate layer protein (CILP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPo256093	XM_020812169.1 Latest version!	Pogona vitticeps cartilage intermediate layer protein (CILP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CILP ( XM_020812168.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020667827.1 Pogona vitticeps cartilage intermediate layer protein (CILP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo256092
Clone ID Related Accession (Same CDS sequence)	XM_020812168.1
Accession Version	XM_020812168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	cartilage intermediate layer protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018151402.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT CCTCGTGGTC AAGGTGGAGC GAATGCTCTG CCAAATGCGA CGAAACTGGT 
ATCCAGATGC GGACCAGGAC CTGCCTTGCG GAAAGTCGCT GGGACCCACG CTGTGAAGAA 
GTGAGCGAGG AAGGGAGGCG ATGCTCCGGA CCTCCATGCG CAGTTTGCAA CATGACTTGC 
TCCATGGGCC GTGTGAACGC AGACTGCAAC GCCTGCATGT GTGAGGAACA TCTGCTTCGT 
GGCAAGGTCT CCCTTCAGGA TGGCGCCCCT GCAGCTGGTG CTGCTGTCTA CCTACAAGCT 
CAGACAGCAA AGTTATTGAC AGTAGCAGAT GATAATGGGG CATTTCAGGT TCCTGGCATC 
TGTCCCAATG GAAAAAACAT CCTGCTGATC AAAAAGCCAA AATATGCCAC TGCTTCGGTT 
ACTGTGCCTG TGTCTTCCAC AAACTCCTCC TCAGTCCATG TTCGCCTAAG ACAATCAGGA 
AAACCATACA TGGTGACACA TCCAGAGAAC AAAGCAAGAA GAGAGGGGCA AAGTGTTTCT 
TTCTGTTGTC ACGCGGATGG AGATCCAGCA CCCAGTCAGT ATTATTGGTT CCACAATGAA 
ACTTTACTGG ACCCTATGCT CTATAAATAT AAAAGCAACC TCGTGCTAAA GAACTTGAAA 
GTGGACCATG CCGGACAGTA TGTCTGCAAG ACCAGCAATG ATGCCGGATC GGTGAAGTCC 
CAAGCTGCCA CGCTCTCTGT AACAGCAAAG AATGAAGCGC CTTGTGTTCA AAAGCCTGAG 
AGCTACCTTA CCCGACTGCC ACACGACTGC TTTCAAAACT CAACCAGCTC CTTCCACTAC 
GACGTCGGTA GGTGCCCCTC CACGCCGTGC TCCGGCATGC AGGACAAAGG CCTCCGGTGC 
AAGGACCCTG TTGCTTACTG CTGTGGGGTC TCCAGAATGG AAACGCGAGA AATCCTGTGC 
GCTGGATACA CCCTTCCCAT CAAAGTGGCT GTGGAGTGTG GGTGTCAGAA ATGCACCAAA 
GCAAAGGTCG TGGTCCGAGG AAGAGCTGTG GCTTCTGATA CAGAGGAGCC CATGAGGTTT 
GGCCATATAT ATCTGGGGAA CAACCGAGTC AGCATGACAG GTTATAAGGG AACCTTCTCA 
GTTCAAGTGC CCCCAAACAC AGAGAGGCTG GTCCTCACAT TTGTTGACCG CATGAATAAG 
TTTGTGAACA CCACTAAAGT GCTGCCATTC AACAGAAACG GTGGAGCTGT CTTCCATGAG 
ATCAAGATGT TAAGAAAGAA GCCGCCTATC ACCCTAGACT CAACTGAGAC CAGCGAGATC 
TCCTTGGGGG ATCTGGAGGG GGGTGATCCG ATAGCCATAC TGGAAATTCC TCCCAATACA 
TTTTACAGGC AGAACGGGCA ACCTTATGTG GGAAAAGTAA AAGCCAGTGT GACCTTCCTA 
GACCCAAGGA ACCTCTCCAG CGCACCCATA GCACAAAGTG ACCTGAATTT TGTGGATGAC 
CAAGGTGACA TGCTCCCACT GAGGACCTAT GGCATGTTTT CTATGGACTT CACTGACGAA 
ACGGCCACAG AGTCCCTCAA TGCAGGAAAA GTGAAAGTCC ACCTGGACTC TTCTCAGGTC 
AAGATGCCAG AACATCTGAC GGGAATGAAG CTTTGGTCCT TAAACCCACA GACCGGTTTA 
TGGGAGGAAG AAGGAGACTT CCATCTTGAC AGCAGCAGAC GGAGCAAGCG AGAAGAGAGA 
ACATTTCTGG TAGGTAATAT GGAGATAAGA GAAAGGCGGC TTTTCAACCT GGATGTACCA 
GAGAGCAGGC GCTGCTACAT TAAGGTGCGC ACTTACAGAA GCGAGAGATA CCTGCCCAGT 
GAGCAAGTTT CAGGCGTGGT GGTTTCCGTT ATCAATTTGG AACCCACTCC GGGATATTCT 
TCTAATCCGA GAGCTTGGGG ACGCTTTGAC AGTGCGGTTA CTGGCTCCGG TGGGGCTTGT 
GTGCCAGCCT TCTGTGACGA CCAAAACCCA GATGCCTATA GTGCTTACAT CATGGCAAGT 
CTTGCGGGAG AAGAACTAGA AGCCGTTCCC TCCTCTCCAA GGCTTAACCC TGCTGCCATT 
GGAGTCCCCC AGCCCTATCT CAACAAACTG AAGTACAGGC GGACGGATCA CACAGATCGT 
CAAGTCAAGA AAACTGCTTT CAGAATCAGT GTGGCCAAAC CCACTCCGAA CGCTGCCGAG 
GAGAGCAATG GCCCCATTTA CCCTTACGAA AAGCTCCAGG AATGTGAAGC TGCTCCATAT 
ACGGCTGCTC ACTTCAGGTT TTACAGGGTA GAGGGAGACA GGTATGATTA TAACACTGTT 
CCTTTTAATG AGGATGACCC TGTGAACTGG ACAGAAGACT ACTTGGCTTG GTGGCCTAGG 
CCAACAGAAT TTAGGGCATG TTATATTAAA GTGAAAATCA ATGGGCCTCT TGAGGTCAAT 
GTGAGATCTC GCAATGCAGG AGGCACCCAT CCACGGACCT CTGGCAAGCT GTATGGCATC 
AGAGATGTTC GTAGCATACG CGATTTGGAC CACCCCAACC TTTCAACAGC ATGTCTGGAA 
TTCAAATGCA GCGGCATGTT GTATGACCAA GAACGCATTG ACCGAACAAT GGTACACATC 
ATCCCACAGG GAAACTGCTA CAGAGAGAGT GTCAACAGCA TGCTTCACGA ATATCTCGTG 
AACCACCTTC CTGTTGTAAT GAATAATGAT TCCAGCCAGT ATACCATGTT GGCGCCTCTT 
GATCCACTTG GCCACAATTA TGGGATCTAC ACAGTGACAG ACCAGGATCC TAGGACAGCC 
AAGGAAATTG CCTTGGGCAG ATGTTTTGAT GGAACATCGG ATGGTTCCTC AAGGATTATG 
AAAAACAATG TGGGCATAGC GCTAACTTTT AACTGTCAAG AGAGAGATGT ACAACAGCAG 
AACCTATTTG AGTCCCTGCG GAACGTTCCT GGATGGTCTC CCGCTAGCTC ACTCCGGGAA 
AGAATAGGTG TACGGAGACC AAGCAGAGGA CGTGGTGTTC TGATCAGGGG ACAAAGTATT 
CCTTTTGTTC GAATGCCACA AAAACGCCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020667827.1
CDS	22..3114
Translation	MLWSSWSRWSECSAKCDETGIQMRTRTCLAESRWDPRCEEVSEEGRRCSGPPCAVCNMTCSMGRVNADCNACMCEEHLLRGKVSLQDGAPAAGAAVYLQAQTAKLLTVADDNGAFQVPGICPNGKNILLIKKPKYATASVTVPVSSTNSSSVHVRLRQSGKPYMVTHPENKARREGQSVSFCCHADGDPAPSQYYWFHNETLLDPMLYKYKSNLVLKNLKVDHAGQYVCKTSNDAGSVKSQAATLSVTAKNEAPCVQKPESYLTRLPHDCFQNSTSSFHYDVGRCPSTPCSGMQDKGLRCKDPVAYCCGVSRMETREILCAGYTLPIKVAVECGCQKCTKAKVVVRGRAVASDTEEPMRFGHIYLGNNRVSMTGYKGTFSVQVPPNTERLVLTFVDRMNKFVNTTKVLPFNRNGGAVFHEIKMLRKKPPITLDSTETSEISLGDLEGGDPIAILEIPPNTFYRQNGQPYVGKVKASVTFLDPRNLSSAPIAQSDLNFVDDQGDMLPLRTYGMFSMDFTDETATESLNAGKVKVHLDSSQVKMPEHLTGMKLWSLNPQTGLWEEEGDFHLDSSRRSKREERTFLVGNMEIRERRLFNLDVPESRRCYIKVRTYRSERYLPSEQVSGVVVSVINLEPTPGYSSNPRAWGRFDSAVTGSGGACVPAFCDDQNPDAYSAYIMASLAGEELEAVPSSPRLNPAAIGVPQPYLNKLKYRRTDHTDRQVKKTAFRISVAKPTPNAAEESNGPIYPYEKLQECEAAPYTAAHFRFYRVEGDRYDYNTVPFNEDDPVNWTEDYLAWWPRPTEFRACYIKVKINGPLEVNVRSRNAGGTHPRTSGKLYGIRDVRSIRDLDHPNLSTACLEFKCSGMLYDQERIDRTMVHIIPQGNCYRESVNSMLHEYLVNHLPVVMNNDSSQYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRIMKNNVGIALTFNCQERDVQQQNLFESLRNVPGWSPASSLRERIGVRRPSRGRGVLIRGQSIPFVRMPQKRQ

Target ORF information:

RefSeq Version	XM_020812168.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps cartilage intermediate layer protein (CILP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020812168.1

ORF Insert Sequence:

ATGCTTTGGT CCTCGTGGTC AAGGTGGAGC GAATGCTCTG CCAAATGCGA CGAAACTGGT 
ATCCAGATGC GGACCAGGAC CTGCCTTGCG GAAAGTCGCT GGGACCCACG CTGTGAAGAA 
GTGAGCGAGG AAGGGAGGCG ATGCTCCGGA CCTCCATGCG CAGTTTGCAA CATGACTTGC 
TCCATGGGCC GTGTGAACGC AGACTGCAAC GCCTGCATGT GTGAGGAACA TCTGCTTCGT 
GGCAAGGTCT CCCTTCAGGA TGGCGCCCCT GCAGCTGGTG CTGCTGTCTA CCTACAAGCT 
CAGACAGCAA AGTTATTGAC AGTAGCAGAT GATAATGGGG CATTTCAGGT TCCTGGCATC 
TGTCCCAATG GAAAAAACAT CCTGCTGATC AAAAAGCCAA AATATGCCAC TGCTTCGGTT 
ACTGTGCCTG TGTCTTCCAC AAACTCCTCC TCAGTCCATG TTCGCCTAAG ACAATCAGGA 
AAACCATACA TGGTGACACA TCCAGAGAAC AAAGCAAGAA GAGAGGGGCA AAGTGTTTCT 
TTCTGTTGTC ACGCGGATGG AGATCCAGCA CCCAGTCAGT ATTATTGGTT CCACAATGAA 
ACTTTACTGG ACCCTATGCT CTATAAATAT AAAAGCAACC TCGTGCTAAA GAACTTGAAA 
GTGGACCATG CCGGACAGTA TGTCTGCAAG ACCAGCAATG ATGCCGGATC GGTGAAGTCC 
CAAGCTGCCA CGCTCTCTGT AACAGCAAAG AATGAAGCGC CTTGTGTTCA AAAGCCTGAG 
AGCTACCTTA CCCGACTGCC ACACGACTGC TTTCAAAACT CAACCAGCTC CTTCCACTAC 
GACGTCGGTA GGTGCCCCTC CACGCCGTGC TCCGGCATGC AGGACAAAGG CCTCCGGTGC 
AAGGACCCTG TTGCTTACTG CTGTGGGGTC TCCAGAATGG AAACGCGAGA AATCCTGTGC 
GCTGGATACA CCCTTCCCAT CAAAGTGGCT GTGGAGTGTG GGTGTCAGAA ATGCACCAAA 
GCAAAGGTCG TGGTCCGAGG AAGAGCTGTG GCTTCTGATA CAGAGGAGCC CATGAGGTTT 
GGCCATATAT ATCTGGGGAA CAACCGAGTC AGCATGACAG GTTATAAGGG AACCTTCTCA 
GTTCAAGTGC CCCCAAACAC AGAGAGGCTG GTCCTCACAT TTGTTGACCG CATGAATAAG 
TTTGTGAACA CCACTAAAGT GCTGCCATTC AACAGAAACG GTGGAGCTGT CTTCCATGAG 
ATCAAGATGT TAAGAAAGAA GCCGCCTATC ACCCTAGACT CAACTGAGAC CAGCGAGATC 
TCCTTGGGGG ATCTGGAGGG GGGTGATCCG ATAGCCATAC TGGAAATTCC TCCCAATACA 
TTTTACAGGC AGAACGGGCA ACCTTATGTG GGAAAAGTAA AAGCCAGTGT GACCTTCCTA 
GACCCAAGGA ACCTCTCCAG CGCACCCATA GCACAAAGTG ACCTGAATTT TGTGGATGAC 
CAAGGTGACA TGCTCCCACT GAGGACCTAT GGCATGTTTT CTATGGACTT CACTGACGAA 
ACGGCCACAG AGTCCCTCAA TGCAGGAAAA GTGAAAGTCC ACCTGGACTC TTCTCAGGTC 
AAGATGCCAG AACATCTGAC GGGAATGAAG CTTTGGTCCT TAAACCCACA GACCGGTTTA 
TGGGAGGAAG AAGGAGACTT CCATCTTGAC AGCAGCAGAC GGAGCAAGCG AGAAGAGAGA 
ACATTTCTGG TAGGTAATAT GGAGATAAGA GAAAGGCGGC TTTTCAACCT GGATGTACCA 
GAGAGCAGGC GCTGCTACAT TAAGGTGCGC ACTTACAGAA GCGAGAGATA CCTGCCCAGT 
GAGCAAGTTT CAGGCGTGGT GGTTTCCGTT ATCAATTTGG AACCCACTCC GGGATATTCT 
TCTAATCCGA GAGCTTGGGG ACGCTTTGAC AGTGCGGTTA CTGGCTCCGG TGGGGCTTGT 
GTGCCAGCCT TCTGTGACGA CCAAAACCCA GATGCCTATA GTGCTTACAT CATGGCAAGT 
CTTGCGGGAG AAGAACTAGA AGCCGTTCCC TCCTCTCCAA GGCTTAACCC TGCTGCCATT 
GGAGTCCCCC AGCCCTATCT CAACAAACTG AAGTACAGGC GGACGGATCA CACAGATCGT 
CAAGTCAAGA AAACTGCTTT CAGAATCAGT GTGGCCAAAC CCACTCCGAA CGCTGCCGAG 
GAGAGCAATG GCCCCATTTA CCCTTACGAA AAGCTCCAGG AATGTGAAGC TGCTCCATAT 
ACGGCTGCTC ACTTCAGGTT TTACAGGGTA GAGGGAGACA GGTATGATTA TAACACTGTT 
CCTTTTAATG AGGATGACCC TGTGAACTGG ACAGAAGACT ACTTGGCTTG GTGGCCTAGG 
CCAACAGAAT TTAGGGCATG TTATATTAAA GTGAAAATCA ATGGGCCTCT TGAGGTCAAT 
GTGAGATCTC GCAATGCAGG AGGCACCCAT CCACGGACCT CTGGCAAGCT GTATGGCATC 
AGAGATGTTC GTAGCATACG CGATTTGGAC CACCCCAACC TTTCAACAGC ATGTCTGGAA 
TTCAAATGCA GCGGCATGTT GTATGACCAA GAACGCATTG ACCGAACAAT GGTACACATC 
ATCCCACAGG GAAACTGCTA CAGAGAGAGT GTCAACAGCA TGCTTCACGA ATATCTCGTG 
AACCACCTTC CTGTTGTAAT GAATAATGAT TCCAGCCAGT ATACCATGTT GGCGCCTCTT 
GATCCACTTG GCCACAATTA TGGGATCTAC ACAGTGACAG ACCAGGATCC TAGGACAGCC 
AAGGAAATTG CCTTGGGCAG ATGTTTTGAT GGAACATCGG ATGGTTCCTC AAGGATTATG 
AAAAACAATG TGGGCATAGC GCTAACTTTT AACTGTCAAG AGAGAGATGT ACAACAGCAG 
AACCTATTTG AGTCCCTGCG GAACGTTCCT GGATGGTCTC CCGCTAGCTC ACTCCGGGAA 
AGAATAGGTG TACGGAGACC AAGCAGAGGA CGTGGTGTTC TGATCAGGGG ACAAAGTATT 
CCTTTTGTTC GAATGCCACA AAAACGCCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP ( XM_020812169.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020667828.1 Pogona vitticeps cartilage intermediate layer protein (CILP), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo256093
Clone ID Related Accession (Same CDS sequence)	XM_020812169.1
Accession Version	XM_020812169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	cartilage intermediate layer protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018151402.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCAA GAACACGGTG CTGTTTTGCT TTCTTACTTC TGCATATGTC CTGCATTGCT 
GGTGAACGGG TTTTAAAGCA GTCGGTCAGC AGGATCCCAC CAGGACAGAA ACCCCAGGGC 
CTTTCAGCCA AAGGCAGTCC AGACAGTAAG TCATTTTCCA TTCTCGGTCC TGGAACATGG 
ACATCTTGGT TCAACATTGA CCATCCTGGA GGCAATGGCG ATTATGAGCG CATGGATGCC 
ATCCGCTTTT ACTACCGGGA GAGGGTATGT CCGCGGCCCA TCCAGATTGA AGTGCGAACA 
ACAGACTGGA TCCCTGCAGC CAACACGGGT CAAGTGGTCC ATGCCAATCC CCAGGAAGGA 
TTCTGGTGTC TGAATAAAGA GCAGCCTGCA GGACAGATGT GTTTGAATTA TGCCGTGCGT 
TTCCTGTGCC CAGCAGCCGC CTTGCCTCAA GATGCCAAAA TGCTTTGGTC CTCGTGGTCA 
AGGTGGAGCG AATGCTCTGC CAAATGCGAT GAAACTGGTA TCCAGATGCG GACCAGGACC 
TGCCTTGCGG AAAGTCGCTG GGACCCACGC TGTGAAGAAG TGAGCGAGGA AGGGAGGCGA 
TGCTCCGGAC CTCCATGCGC AGTTTGCAAC ATGACTTGCT CCATGGGCCG TGTGAACGCA 
GACTGCAACG CCTGCATGTG TGAGGAACAT CTGCTTCGTG GCAAGGTCTC CCTTCAGGAT 
GGCGCCCCTG CAGCTGGTGC TGCTGTCTAC CTACAAGCTC AGACAGCAAA GTTATTGACA 
GTAGCAGATG ATAATGGGGC ATTTCAGGTT CCTGGCATCT GTCCCAATGG AAAAAACATC 
CTGCTGATCA AAAAGCCAAA ATATGCCACT GCTTCGGTTA CTGTGCCTGT GTCTTCCACA 
AACTCCTCCT CAGTCCATGT TCGCCTAAGA CAATCAGGAA AACCATACAT GGTGACACAT 
CCAGAGAACA AAGCAAGAAG AGAGGGGCAA AGTGTTTCTT TCTGTTGTCA CGCGGATGGA 
GATCCAGCAC CCAGTCAGTA TTATTGGTTC CACAATGAAA CTTTACTGGA CCCTATGCTC 
TATAAATATA AAAGCAACCT CGTGCTAAAG AACTTGAAAG TGGACCATGC CGGACAGTAT 
GTCTGCAAGA CCAGCAATGA TGCCGGATCG GTGAAGTCCC AAGCTGCCAC GCTCTCTGTA 
ACAGCAAAGA ATGAAGCGCC TTGTGTTCAA AAGCCTGAGA GCTACCTTAC CCGACTGCCA 
CACGACTGCT TTCAAAACTC AACCAGCTCC TTCCACTACG ACGTCGGTAG GTGCCCCTCC 
ACGCCGTGCT CCGGCATGCA GGACAAAGGC CTCCGGTGCA AGGACCCTGT TGCTTACTGC 
TGTGGGGTCT CCAGAATGGA AACGCGAGAA ATCCTGTGCG CTGGATACAC CCTTCCCATC 
AAAGTGGCTG TGGAGTGTGG GTGTCAGAAA TGCACCAAAG CAAAGGTCGT GGTCCGAGGA 
AGAGCTGTGG CTTCTGATAC AGAGGAGCCC ATGAGGTTTG GCCATATATA TCTGGGGAAC 
AACCGAGTCA GCATGACAGG TTATAAGGGA ACCTTCTCAG TTCAAGTGCC CCCAAACACA 
GAGAGGCTGG TCCTCACATT TGTTGACCGC ATGAATAAGT TTGTGAACAC CACTAAAGTG 
CTGCCATTCA ACAGAAACGG TGGAGCTGTC TTCCATGAGA TCAAGATGTT AAGAAAGAAG 
CCGCCTATCA CCCTAGACTC AACTGAGACC AGCGAGATCT CCTTGGGGGA TCTGGAGGGG 
GGTGATCCGA TAGCCATACT GGAAATTCCT CCCAATACAT TTTACAGGCA GAACGGGCAA 
CCTTATGTGG GAAAAGTAAA AGCCAGTGTG ACCTTCCTAG ACCCAAGGAA CCTCTCCAGC 
GCACCCATAG CACAAAGTGA CCTGAATTTT GTGGATGACC AAGGTGACAT GCTCCCACTG 
AGGACCTATG GCATGTTTTC TATGGACTTC ACTGACGAAA CGGCCACAGA GTCCCTCAAT 
GCAGGAAAAG TGAAAGTCCA CCTGGACTCT TCTCAGGTCA AGATGCCAGA ACATCTGACG 
GGAATGAAGC TTTGGTCCTT AAACCCACAG ACCGGTTTAT GGGAGGAAGA AGGAGACTTC 
CATCTTGACA GCAGCAGACG GAGCAAGCGA GAAGAGAGAA CATTTCTGGT AGGTAATATG 
GAGATAAGAG AAAGGCGGCT TTTCAACCTG GATGTACCAG AGAGCAGGCG CTGCTACATT 
AAGGTGCGCA CTTACAGAAG CGAGAGATAC CTGCCCAGTG AGCAAGTTTC AGGCGTGGTG 
GTTTCCGTTA TCAATTTGGA ACCCACTCCG GGATATTCTT CTAATCCGAG AGCTTGGGGA 
CGCTTTGACA GTGCGGTTAC TGGCTCCGGT GGGGCTTGTG TGCCAGCCTT CTGTGACGAC 
CAAAACCCAG ATGCCTATAG TGCTTACATC ATGGCAAGTC TTGCGGGAGA AGAACTAGAA 
GCCGTTCCCT CCTCTCCAAG GCTTAACCCT GCTGCCATTG GAGTCCCCCA GCCCTATCTC 
AACAAACTGA AGTACAGGCG GACGGATCAC ACAGATCGTC AAGTCAAGAA AACTGCTTTC 
AGAATCAGTG TGGCCAAACC CACTCCGAAC GCTGCCGAGG AGAGCAATGG CCCCATTTAC 
CCTTACGAAA AGCTCCAGGA ATGTGAAGCT GCTCCATATA CGGCTGCTCA CTTCAGGTTT 
TACAGGGTAG AGGGAGACAG GTATGATTAT AACACTGTTC CTTTTAATGA GGATGACCCT 
GTGAACTGGA CAGAAGACTA CTTGGCTTGG TGGCCTAGGC CAACAGAATT TAGGGCATGT 
TATATTAAAG TGAAAATCAA TGGGCCTCTT GAGGTCAATG TGAGATCTCG CAATGCAGGA 
GGCACCCATC CACGGACCTC TGGCAAGCTG TATGGCATCA GAGATGTTCG TAGCATACGC 
GATTTGGACC ACCCCAACCT TTCAACAGCA TGTCTGGAAT TCAAATGCAG CGGCATGTTG 
TATGACCAAG AACGCATTGA CCGAACAATG GTACACATCA TCCCACAGGG AAACTGCTAC 
AGAGAGAGTG TCAACAGCAT GCTTCACGAA TATCTCGTGA ACCACCTTCC TGTTGTAATG 
AATAATGATT CCAGCCAGTA TACCATGTTG GCGCCTCTTG ATCCACTTGG CCACAATTAT 
GGGATCTACA CAGTGACAGA CCAGGATCCT AGGACAGCCA AGGAAATTGC CTTGGGCAGA 
TGTTTTGATG GAACATCGGA TGGTTCCTCA AGGATTATGA AAAACAATGT GGGCATAGCG 
CTAACTTTTA ACTGTCAAGA GAGAGATGTA CAACAGCAGA ACCTATTTGA GTCCCTGCGG 
AACGTTCCTG GATGGTCTCC CGCTAGCTCA CTCCGGGAAA GAATAGGTGT ACGGAGACCA 
AGCAGAGGAC GTGGTGTTCT GATCAGGGGA CAAAGTATTC CTTTTGTTCG AATGCCACAA 
AAACGCCAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020667828.1
CDS	189..3740
Translation	MDARTRCCFAFLLLHMSCIAGERVLKQSVSRIPPGQKPQGLSAKGSPDSKSFSILGPGTWTSWFNIDHPGGNGDYERMDAIRFYYRERVCPRPIQIEVRTTDWIPAANTGQVVHANPQEGFWCLNKEQPAGQMCLNYAVRFLCPAAALPQDAKMLWSSWSRWSECSAKCDETGIQMRTRTCLAESRWDPRCEEVSEEGRRCSGPPCAVCNMTCSMGRVNADCNACMCEEHLLRGKVSLQDGAPAAGAAVYLQAQTAKLLTVADDNGAFQVPGICPNGKNILLIKKPKYATASVTVPVSSTNSSSVHVRLRQSGKPYMVTHPENKARREGQSVSFCCHADGDPAPSQYYWFHNETLLDPMLYKYKSNLVLKNLKVDHAGQYVCKTSNDAGSVKSQAATLSVTAKNEAPCVQKPESYLTRLPHDCFQNSTSSFHYDVGRCPSTPCSGMQDKGLRCKDPVAYCCGVSRMETREILCAGYTLPIKVAVECGCQKCTKAKVVVRGRAVASDTEEPMRFGHIYLGNNRVSMTGYKGTFSVQVPPNTERLVLTFVDRMNKFVNTTKVLPFNRNGGAVFHEIKMLRKKPPITLDSTETSEISLGDLEGGDPIAILEIPPNTFYRQNGQPYVGKVKASVTFLDPRNLSSAPIAQSDLNFVDDQGDMLPLRTYGMFSMDFTDETATESLNAGKVKVHLDSSQVKMPEHLTGMKLWSLNPQTGLWEEEGDFHLDSSRRSKREERTFLVGNMEIRERRLFNLDVPESRRCYIKVRTYRSERYLPSEQVSGVVVSVINLEPTPGYSSNPRAWGRFDSAVTGSGGACVPAFCDDQNPDAYSAYIMASLAGEELEAVPSSPRLNPAAIGVPQPYLNKLKYRRTDHTDRQVKKTAFRISVAKPTPNAAEESNGPIYPYEKLQECEAAPYTAAHFRFYRVEGDRYDYNTVPFNEDDPVNWTEDYLAWWPRPTEFRACYIKVKINGPLEVNVRSRNAGGTHPRTSGKLYGIRDVRSIRDLDHPNLSTACLEFKCSGMLYDQERIDRTMVHIIPQGNCYRESVNSMLHEYLVNHLPVVMNNDSSQYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRIMKNNVGIALTFNCQERDVQQQNLFESLRNVPGWSPASSLRERIGVRRPSRGRGVLIRGQSIPFVRMPQKRQ

Target ORF information:

RefSeq Version	XM_020812169.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps cartilage intermediate layer protein (CILP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020812169.1

ORF Insert Sequence:

ATGGACGCAA GAACACGGTG CTGTTTTGCT TTCTTACTTC TGCATATGTC CTGCATTGCT 
GGTGAACGGG TTTTAAAGCA GTCGGTCAGC AGGATCCCAC CAGGACAGAA ACCCCAGGGC 
CTTTCAGCCA AAGGCAGTCC AGACAGTAAG TCATTTTCCA TTCTCGGTCC TGGAACATGG 
ACATCTTGGT TCAACATTGA CCATCCTGGA GGCAATGGCG ATTATGAGCG CATGGATGCC 
ATCCGCTTTT ACTACCGGGA GAGGGTATGT CCGCGGCCCA TCCAGATTGA AGTGCGAACA 
ACAGACTGGA TCCCTGCAGC CAACACGGGT CAAGTGGTCC ATGCCAATCC CCAGGAAGGA 
TTCTGGTGTC TGAATAAAGA GCAGCCTGCA GGACAGATGT GTTTGAATTA TGCCGTGCGT 
TTCCTGTGCC CAGCAGCCGC CTTGCCTCAA GATGCCAAAA TGCTTTGGTC CTCGTGGTCA 
AGGTGGAGCG AATGCTCTGC CAAATGCGAT GAAACTGGTA TCCAGATGCG GACCAGGACC 
TGCCTTGCGG AAAGTCGCTG GGACCCACGC TGTGAAGAAG TGAGCGAGGA AGGGAGGCGA 
TGCTCCGGAC CTCCATGCGC AGTTTGCAAC ATGACTTGCT CCATGGGCCG TGTGAACGCA 
GACTGCAACG CCTGCATGTG TGAGGAACAT CTGCTTCGTG GCAAGGTCTC CCTTCAGGAT 
GGCGCCCCTG CAGCTGGTGC TGCTGTCTAC CTACAAGCTC AGACAGCAAA GTTATTGACA 
GTAGCAGATG ATAATGGGGC ATTTCAGGTT CCTGGCATCT GTCCCAATGG AAAAAACATC 
CTGCTGATCA AAAAGCCAAA ATATGCCACT GCTTCGGTTA CTGTGCCTGT GTCTTCCACA 
AACTCCTCCT CAGTCCATGT TCGCCTAAGA CAATCAGGAA AACCATACAT GGTGACACAT 
CCAGAGAACA AAGCAAGAAG AGAGGGGCAA AGTGTTTCTT TCTGTTGTCA CGCGGATGGA 
GATCCAGCAC CCAGTCAGTA TTATTGGTTC CACAATGAAA CTTTACTGGA CCCTATGCTC 
TATAAATATA AAAGCAACCT CGTGCTAAAG AACTTGAAAG TGGACCATGC CGGACAGTAT 
GTCTGCAAGA CCAGCAATGA TGCCGGATCG GTGAAGTCCC AAGCTGCCAC GCTCTCTGTA 
ACAGCAAAGA ATGAAGCGCC TTGTGTTCAA AAGCCTGAGA GCTACCTTAC CCGACTGCCA 
CACGACTGCT TTCAAAACTC AACCAGCTCC TTCCACTACG ACGTCGGTAG GTGCCCCTCC 
ACGCCGTGCT CCGGCATGCA GGACAAAGGC CTCCGGTGCA AGGACCCTGT TGCTTACTGC 
TGTGGGGTCT CCAGAATGGA AACGCGAGAA ATCCTGTGCG CTGGATACAC CCTTCCCATC 
AAAGTGGCTG TGGAGTGTGG GTGTCAGAAA TGCACCAAAG CAAAGGTCGT GGTCCGAGGA 
AGAGCTGTGG CTTCTGATAC AGAGGAGCCC ATGAGGTTTG GCCATATATA TCTGGGGAAC 
AACCGAGTCA GCATGACAGG TTATAAGGGA ACCTTCTCAG TTCAAGTGCC CCCAAACACA 
GAGAGGCTGG TCCTCACATT TGTTGACCGC ATGAATAAGT TTGTGAACAC CACTAAAGTG 
CTGCCATTCA ACAGAAACGG TGGAGCTGTC TTCCATGAGA TCAAGATGTT AAGAAAGAAG 
CCGCCTATCA CCCTAGACTC AACTGAGACC AGCGAGATCT CCTTGGGGGA TCTGGAGGGG 
GGTGATCCGA TAGCCATACT GGAAATTCCT CCCAATACAT TTTACAGGCA GAACGGGCAA 
CCTTATGTGG GAAAAGTAAA AGCCAGTGTG ACCTTCCTAG ACCCAAGGAA CCTCTCCAGC 
GCACCCATAG CACAAAGTGA CCTGAATTTT GTGGATGACC AAGGTGACAT GCTCCCACTG 
AGGACCTATG GCATGTTTTC TATGGACTTC ACTGACGAAA CGGCCACAGA GTCCCTCAAT 
GCAGGAAAAG TGAAAGTCCA CCTGGACTCT TCTCAGGTCA AGATGCCAGA ACATCTGACG 
GGAATGAAGC TTTGGTCCTT AAACCCACAG ACCGGTTTAT GGGAGGAAGA AGGAGACTTC 
CATCTTGACA GCAGCAGACG GAGCAAGCGA GAAGAGAGAA CATTTCTGGT AGGTAATATG 
GAGATAAGAG AAAGGCGGCT TTTCAACCTG GATGTACCAG AGAGCAGGCG CTGCTACATT 
AAGGTGCGCA CTTACAGAAG CGAGAGATAC CTGCCCAGTG AGCAAGTTTC AGGCGTGGTG 
GTTTCCGTTA TCAATTTGGA ACCCACTCCG GGATATTCTT CTAATCCGAG AGCTTGGGGA 
CGCTTTGACA GTGCGGTTAC TGGCTCCGGT GGGGCTTGTG TGCCAGCCTT CTGTGACGAC 
CAAAACCCAG ATGCCTATAG TGCTTACATC ATGGCAAGTC TTGCGGGAGA AGAACTAGAA 
GCCGTTCCCT CCTCTCCAAG GCTTAACCCT GCTGCCATTG GAGTCCCCCA GCCCTATCTC 
AACAAACTGA AGTACAGGCG GACGGATCAC ACAGATCGTC AAGTCAAGAA AACTGCTTTC 
AGAATCAGTG TGGCCAAACC CACTCCGAAC GCTGCCGAGG AGAGCAATGG CCCCATTTAC 
CCTTACGAAA AGCTCCAGGA ATGTGAAGCT GCTCCATATA CGGCTGCTCA CTTCAGGTTT 
TACAGGGTAG AGGGAGACAG GTATGATTAT AACACTGTTC CTTTTAATGA GGATGACCCT 
GTGAACTGGA CAGAAGACTA CTTGGCTTGG TGGCCTAGGC CAACAGAATT TAGGGCATGT 
TATATTAAAG TGAAAATCAA TGGGCCTCTT GAGGTCAATG TGAGATCTCG CAATGCAGGA 
GGCACCCATC CACGGACCTC TGGCAAGCTG TATGGCATCA GAGATGTTCG TAGCATACGC 
GATTTGGACC ACCCCAACCT TTCAACAGCA TGTCTGGAAT TCAAATGCAG CGGCATGTTG 
TATGACCAAG AACGCATTGA CCGAACAATG GTACACATCA TCCCACAGGG AAACTGCTAC 
AGAGAGAGTG TCAACAGCAT GCTTCACGAA TATCTCGTGA ACCACCTTCC TGTTGTAATG 
AATAATGATT CCAGCCAGTA TACCATGTTG GCGCCTCTTG ATCCACTTGG CCACAATTAT 
GGGATCTACA CAGTGACAGA CCAGGATCCT AGGACAGCCA AGGAAATTGC CTTGGGCAGA 
TGTTTTGATG GAACATCGGA TGGTTCCTCA AGGATTATGA AAAACAATGT GGGCATAGCG 
CTAACTTTTA ACTGTCAAGA GAGAGATGTA CAACAGCAGA ACCTATTTGA GTCCCTGCGG 
AACGTTCCTG GATGGTCTCC CGCTAGCTCA CTCCGGGAAA GAATAGGTGT ACGGAGACCA 
AGCAGAGGAC GTGGTGTTCT GATCAGGGGA CAAAGTATTC CTTTTGTTCG AATGCCACAA 
AAACGCCAAT AA