SIRT1 cDNA ORF clone, Pogona vitticeps(central bearded dragon)

Gene
Clones
gRNAs

Summary

Gene Symbol	SIRT1
Entrez Gene ID	110087595
Full Name	sirtuin 1
Gene Type	protein-coding
Organism	Pogona vitticeps(central bearded dragon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020809413.1	XP_020665072.1	NAD-dependent protein deacetylase sirtuin-1

Pathways from BioSystems
Gene Ontology

The following SIRT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIRT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo254175	XM_020809413.1 Latest version!	Pogona vitticeps sirtuin 1 (SIRT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIRT1 ( XM_020809413.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020665072.1 Pogona vitticeps sirtuin 1 (SIRT1), mRNA.

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CloneID	OPo254175
Clone ID Related Accession (Same CDS sequence)	XM_020809413.1
Accession Version	XM_020809413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	NAD-dependent protein deacetylase sirtuin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018151237.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCGGATG AGGAGACGCC ACTCCTCCAG TCCCGCAGCG GCGGCCGCGG CAGCAGCAAC 
GGCAGTGTGG CGGCCCCGGT CGCAGAGAGC CCAGAGCCAG CTCTCAAGCG GCCGCGCCGG 
GGCCCGACTG ACAGCCCCGG GCCGGACTGT GAGACCGCGA GAGGAGCCGC CGGAGAGGCA 
GAGACGGCGC CTCCCGCAGG AAGCACCGCC GAGGAGGAGG AGGAGGAAGA GGCCCGGAAG 
GTGGAGGAGG TTGCTGCCGC CGCCGCCGCT GTTGTTGCCG CCGCCGCTGC GGAGTCGGGA 
GAGGTTTCAG CGGCACCCGG GAGACAAAGC GGCGGCGAAG GCTGGGACTG CGCAGAGAGT 
GGGATGTATG TTGACTGTGA TAATATATTT TTTAGTGATG AAATCATTGC AAACGGCTTC 
CATTCTTGTG AGAGTGATGA AGATGACAGA GCTTCTCATG CAAGCTCTAG TGACTGGACT 
CCACGGCCAC GTATAGGTCC ATATACTTTT GTCCAACAGC ATCTCATGAT AGGCACAGAT 
CCCCGTGCAA TTCTGAAAGA TTTGTTACCA GAAACAGTTC CTCCCCCTGA ACTGGATGAT 
ATGACATTAT GGCAAATCGT TATTAATATT CTGTCAGAAC CACCTAAAAG AAAAAAGAGG 
AAAGATATTA ATACGTTAGA GGATGCTGTG AAACTCCTAC ATGAATGCAA AAAAATAATT 
GTCTTGACTG GAGCTGGGGT GTCTGTGTCT TGTGGAATTC CTGACTTCCG GTCAAGAGAT 
GGTATCTATG CACGCCTTGC TGTGGATTTC CCAGATCTTC CTGATCCTCA AGCAATGTTT 
GATATTGAAT ACTTCAGGAA AGATCCAAGG CCATTTTTTA AATTTGCAAA GGAAATATAT 
CCTGGACAGT TTCAACCATC TCTCTGTCAT AAATGTATGG CTTTGCTGGA TAAAGAAAAA 
AAACTGCTTC ATAATTATAC ACAGAACATA GATACAATGG AACAAGTTGC AGGAATTCAA 
AGGATAATCC AGTGTCATGG TTCATTTGCA ACTGCTTCTT GCCTCATTTG TAAATACAAA 
GTTGATTGTG AAGTTGTTCG AGCAGATATT TTTAATCAGG TTGTTCCTAG ATGTCCCAGA 
TGTCCACCTG ATGAACCACT TGCCATCATA AAGCCAGAAA TAGTGTTCTT TGGTGAAAAT 
CTGCCTGAGC AGTTTCATAG GGCCATGAAA TATGACAAAG ATGAGGTTGA TCTTCTTATT 
GTTATTGGGT CTTCACTTAA AGTAAGACCG GTAGCATTAA TTCCAAGTTC CATCCCACAT 
GAAGTGCCTC AGATTTTAAT TAATAGGGAA CCCTTGCCTC ATCTACACTT CGATGTGGAG 
CTTCTTGGTG ACTGTGATGT TATTATCAAT GAGTTGTGTC ATAGATTAGG TGGTGCATAT 
GCAAAACTTT GTAACAGTTC AGTCAAACTT TCAGAAATTG CAGAGAAACC TCCACGACCA 
CTCAAAGAAT TTGAAATACA TTCAGTTGAG TTACCACCTA CTCCGCTAAA CGTTTCTGAA 
TATTCTAGTT CACCTGAGAG AATTATCCCA CAAGATTCTC AGGTAGTACG TTCAGAGCAT 
TCTTCCGAAT TTAAAGTAGG AGATTCAGAA GTTGCCTTGG AATCTAAAGA GTACTGTATG 
GAGGAAAAGT CACATGAGGT ACAAAACTGT TTAGAAAACA CTGACAGTCT TCCTGGCCAG 
TTAGAAGACC CAGAACATGT GAAGGAACAT GGATCTAACC AAGGAGAAAA TAAAGATAAA 
AGTGAAAAAA CATCTGTTGA AGCACTGAGA AAATGTTGGG TTAACAAATG TCCAAAAGAG 
CAGATCAGCA AGCGTCTTGA TGGTACCCAA TACTTGTTTG TACCACCAAA TCGGTATATA 
TTCCATGGTG CTGAAGTATT CTCGGACTCA GAAGATGATG TCATATCCTC CAGCTCTTGT 
GGAAGTAGTA GCGATAGTGG GTCCTGTCAT AGTCCAAGTT TAGACATAGA AGATGAGAGT 
GAAATTGAAG AATTATATAA CGGCATAGAA GAAGATGCAC CTGATAGGGA AGATGAGAAT 
GGATTTGGGG AAGATGGAAT TCAAGACTCT GTGGATGTGT CAGTTCCTGT AAATGAGTCT 
GCAGGATTTG ACCATTCGGC AGACAAACTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020665072.1
CDS	372..2564
Translation	MADEETPLLQSRSGGRGSSNGSVAAPVAESPEPALKRPRRGPTDSPGPDCETARGAAGEAETAPPAGSTAEEEEEEEARKVEEVAAAAAAVVAAAAAESGEVSAAPGRQSGGEGWDCAESGMYVDCDNIFFSDEIIANGFHSCESDEDDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRAILKDLLPETVPPPELDDMTLWQIVINILSEPPKRKKRKDINTLEDAVKLLHECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKCMALLDKEKKLLHNYTQNIDTMEQVAGIQRIIQCHGSFATASCLICKYKVDCEVVRADIFNQVVPRCPRCPPDEPLAIIKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGAYAKLCNSSVKLSEIAEKPPRPLKEFEIHSVELPPTPLNVSEYSSSPERIIPQDSQVVRSEHSSEFKVGDSEVALESKEYCMEEKSHEVQNCLENTDSLPGQLEDPEHVKEHGSNQGENKDKSEKTSVEALRKCWVNKCPKEQISKRLDGTQYLFVPPNRYIFHGAEVFSDSEDDVISSSSCGSSSDSGSCHSPSLDIEDESEIEELYNGIEEDAPDREDENGFGEDGIQDSVDVSVPVNESAGFDHSADKL

Target ORF information:

RefSeq Version	XM_020809413.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps sirtuin 1 (SIRT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020809413.1

ORF Insert Sequence:

ATGGCGGATG AGGAGACGCC ACTCCTCCAG TCCCGCAGCG GCGGCCGCGG CAGCAGCAAC 
GGCAGTGTGG CGGCCCCGGT CGCAGAGAGC CCAGAGCCAG CTCTCAAGCG GCCGCGCCGG 
GGCCCGACTG ACAGCCCCGG GCCGGACTGT GAGACCGCGA GAGGAGCCGC CGGAGAGGCA 
GAGACGGCGC CTCCCGCAGG AAGCACCGCC GAGGAGGAGG AGGAGGAAGA GGCCCGGAAG 
GTGGAGGAGG TTGCTGCCGC CGCCGCCGCT GTTGTTGCCG CCGCCGCTGC GGAGTCGGGA 
GAGGTTTCAG CGGCACCCGG GAGACAAAGC GGCGGCGAAG GCTGGGACTG CGCAGAGAGT 
GGGATGTATG TTGACTGTGA TAATATATTT TTTAGTGATG AAATCATTGC AAACGGCTTC 
CATTCTTGTG AGAGTGATGA AGATGACAGA GCTTCTCATG CAAGCTCTAG TGACTGGACT 
CCACGGCCAC GTATAGGTCC ATATACTTTT GTCCAACAGC ATCTCATGAT AGGCACAGAT 
CCCCGTGCAA TTCTGAAAGA TTTGTTACCA GAAACAGTTC CTCCCCCTGA ACTGGATGAT 
ATGACATTAT GGCAAATCGT TATTAATATT CTGTCAGAAC CACCTAAAAG AAAAAAGAGG 
AAAGATATTA ATACGTTAGA GGATGCTGTG AAACTCCTAC ATGAATGCAA AAAAATAATT 
GTCTTGACTG GAGCTGGGGT GTCTGTGTCT TGTGGAATTC CTGACTTCCG GTCAAGAGAT 
GGTATCTATG CACGCCTTGC TGTGGATTTC CCAGATCTTC CTGATCCTCA AGCAATGTTT 
GATATTGAAT ACTTCAGGAA AGATCCAAGG CCATTTTTTA AATTTGCAAA GGAAATATAT 
CCTGGACAGT TTCAACCATC TCTCTGTCAT AAATGTATGG CTTTGCTGGA TAAAGAAAAA 
AAACTGCTTC ATAATTATAC ACAGAACATA GATACAATGG AACAAGTTGC AGGAATTCAA 
AGGATAATCC AGTGTCATGG TTCATTTGCA ACTGCTTCTT GCCTCATTTG TAAATACAAA 
GTTGATTGTG AAGTTGTTCG AGCAGATATT TTTAATCAGG TTGTTCCTAG ATGTCCCAGA 
TGTCCACCTG ATGAACCACT TGCCATCATA AAGCCAGAAA TAGTGTTCTT TGGTGAAAAT 
CTGCCTGAGC AGTTTCATAG GGCCATGAAA TATGACAAAG ATGAGGTTGA TCTTCTTATT 
GTTATTGGGT CTTCACTTAA AGTAAGACCG GTAGCATTAA TTCCAAGTTC CATCCCACAT 
GAAGTGCCTC AGATTTTAAT TAATAGGGAA CCCTTGCCTC ATCTACACTT CGATGTGGAG 
CTTCTTGGTG ACTGTGATGT TATTATCAAT GAGTTGTGTC ATAGATTAGG TGGTGCATAT 
GCAAAACTTT GTAACAGTTC AGTCAAACTT TCAGAAATTG CAGAGAAACC TCCACGACCA 
CTCAAAGAAT TTGAAATACA TTCAGTTGAG TTACCACCTA CTCCGCTAAA CGTTTCTGAA 
TATTCTAGTT CACCTGAGAG AATTATCCCA CAAGATTCTC AGGTAGTACG TTCAGAGCAT 
TCTTCCGAAT TTAAAGTAGG AGATTCAGAA GTTGCCTTGG AATCTAAAGA GTACTGTATG 
GAGGAAAAGT CACATGAGGT ACAAAACTGT TTAGAAAACA CTGACAGTCT TCCTGGCCAG 
TTAGAAGACC CAGAACATGT GAAGGAACAT GGATCTAACC AAGGAGAAAA TAAAGATAAA 
AGTGAAAAAA CATCTGTTGA AGCACTGAGA AAATGTTGGG TTAACAAATG TCCAAAAGAG 
CAGATCAGCA AGCGTCTTGA TGGTACCCAA TACTTGTTTG TACCACCAAA TCGGTATATA 
TTCCATGGTG CTGAAGTATT CTCGGACTCA GAAGATGATG TCATATCCTC CAGCTCTTGT 
GGAAGTAGTA GCGATAGTGG GTCCTGTCAT AGTCCAAGTT TAGACATAGA AGATGAGAGT 
GAAATTGAAG AATTATATAA CGGCATAGAA GAAGATGCAC CTGATAGGGA AGATGAGAAT 
GGATTTGGGG AAGATGGAAT TCAAGACTCT GTGGATGTGT CAGTTCCTGT AAATGAGTCT 
GCAGGATTTG ACCATTCGGC AGACAAACTG TAA