ESPN cDNA ORF clone, Pogona vitticeps(central bearded dragon)

Gene
Clones
gRNAs

Summary

Gene Symbol	ESPN
Entrez Gene ID	110082452
Full Name	espin
Gene Type	protein-coding
Organism	Pogona vitticeps(central bearded dragon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020800002.1	XP_020655661.1	LOW QUALITY PROTEIN: espin

Pathways from BioSystems
Gene Ontology

The following ESPN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESPN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo247491	XM_020800002.1 Latest version!	Pogona vitticeps espin (ESPN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ESPN ( XM_020800002.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020655661.1 Pogona vitticeps espin (ESPN), mRNA.

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CloneID	OPo247491
Clone ID Related Accession (Same CDS sequence)	XM_020800002.1
Accession Version	XM_020800002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	LOW QUALITY PROTEIN: espin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018150956.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGAGCTATG TCACCCACGC CTTTTGCGCC GCAGACACGG ACCATTCTGG TGCCACCGTC 
TTACACCTGG CCGCCCGGTT TGGCCACCAC GAGGTGATCG ACTGGCTTCT GCGCTTCGGG 
GGCAGCGACC CGCTGGCGGC CACGGACACG GGGGCTCTCC CTGTTCACTA CGCTGCGGCC 
AAAGGAGACT TCCCTTCCTT GAGACTCCTC ATCGGGCATT GCCCCAGTTC GGTCACTGCC 
CAAACCAAGA ATGGTGCCAC GCCACTTTAC TTAGCCTGCC AGGAAGGCCA CCTGGAAATC 
ATCCAGTATC TGGTGAAGGA CTGCGGAGCG GATCCCCACG TACGCGCCAA TGACGGGATG 
ACCCCTCTCC ACGCTGCGGC CCAGATGGGA CACAACACCG TCATCGTTTG GCTGATGAGC 
TTCACCGACA TCAGCCTCTC CGAGCAGGAC GACGATGGGG CCACCGCCAT GCACTTTGCC 
GCCAGCCGGG GCCATGCCAA GGTGCTCAGC TGGCTGCTGC TGCACGGAGG GGAGATCACC 
ACAGACCACT GGGGAGGGAC CCCCTTGCAC GACGCGGCCG AGAACGGAGA GCTGGAGTGT 
TGCCAAATCT TAGTGGTGAA TGGCGTGAAC CTCGGCATCC GAGACCAGGA TGGCTACACG 
GCTGCCGACC TTGCAGACTA CAATGGGCAC GTGCACTGCG CCAAGTACCT GCGTACGGTG 
GAGAACATGA GCGTGGAACA CCGGGTCCTC TCGCGAGACC CGTCTACCGA CCTGGAGTTC 
AAGCAGCCCG ATTCGGGGAT GTCTTCGCCC AACACCACCG TGTCTGCCCC ACAGGCCCAC 
TTCAACCTGG GTTCGCCTAG CAGCACCCTC TCCAACTACG ACTCCTGCAA CTCCAGTCAG 
TCCAGCACAG GAGAGAAACG GAACAGCCAG GCCTCCCGTA AGCATCAGGC GGGGGGAGAC 
CCTCCCTCGG ACGGCGCTTC CCGAATTCCT GAAACGACCA TCACGGACAT GCAGACCTAT 
ATGGACATGC TGAATCCTCC CGAAGTCCAG CACACCGCGG AAATCTACGT CCAGGCTAAG 
AACAACCTCC GTCACGTGGA GAGCGAAGTG TTGAAGCGAG AGCTTACCTC TCTGGAGAAC 
AGCCCGGAGC TACGGCGCGT CGACTCCAGC CGGAAGTCGA GAAACTTCAA CAAGCAGCCC 
AGCACGGGCG ATTACTACAA ACATCTGGGC GGCGGCGAAC CAGCTGGGAG CAACCGGAAG 
ATGGCCCACA GCGAGGAGGC TTCGCTTCTG TCCGAGGAGA GTGTGCAAAA TGGGAGTGCG 
GCAGAGAGCA AGCCTTCCGG CGAGGTGCCT CCCGCCGAGA ACCACCCGGC TTCGACCAGC 
CAACGGCGCT CCTCCTCGTC GACTGGAAGC ACCAAATCCT TCAACATGAT GTCCCCGACC 
GGAGACAACG CAGAGCTGCT GGCTGAGATC AAGGCTGGGA AGAGCCTCAA GCCGACCCCA 
CAGAGCAAGG GCTTCACCAC CGTCTTCTCC GGCTGCGGCC AGGCCGGCGC CAACGTGGAG 
ACTCCGGCAT CCTCCCCTTC CCCAACCAGG AGCCCAACTC CTCCTTCTAC CCCCGAAGCA 
GCTTCTGCCC CCCGGCCTGC CACCTCGCCT GAACCGGTCG TGAACGGCTC CTTGCCGCCC 
CTCGCCTCCT CAGCCCCAGC CGGCCAAGTG GACCTCGAGG GCCTGATCCC CACCCACGAT 
GAGCAAGGCA GGCCCATCCC GGAGTGGAAG CGCCAGGTGA TGGTCCGGAA GCTCCAGATG 
AAGATCCAGG AGGAGGAGGA GCAGAGACGC AAGGAACGAG AAGAAGAGGC TCGCCTGGCT 
ACCATGCCCG CCTGGCGGAG AGACATCCTT CGCAAGAAGA TGGAAGAGGA AAGAGAGCAA 
AAACGAAAAG AGCAGGAGAA ACTGAGGCGG GAAGAGGAGG AGAAAGAGAA AGAGCAGTCG 
GAAAAGCTAA GGACTCTTGG TTACGATGAG ACTAAGCTGG CGCCCTGGCA AAGGCAGATC 
ATCCTCAAGA AAGGAGACAT CGCCAAGCAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020655661.1
CDS	1..2073
Translation	MSYVTHAFCAADTDHSGATVLHLAARFGHHEVIDWLLRFGGSDPLAATDTGALPVHYAAAKGDFPSLRLLIGHCPSSVTAQTKNGATPLYLACQEGHLEIIQYLVKDCGADPHVRANDGMTPLHAAAQMGHNTVIVWLMSFTDISLSEQDDDGATAMHFAASRGHAKVLSWLLLHGGEITTDHWGGTPLHDAAENGELECCQILVVNGVNLGIRDQDGYTAADLADYNGHVHCAKYLRTVENMSVEHRVLSRDPSTDLEFKQPDSGMSSPNTTVSAPQAHFNLGSPSSTLSNYDSCNSSQSSTGEKRNSQASRKHQAGGDPPSDGASRIPETTITDMQTYMDMLNPPEVQHTAEIYVQAKNNLRHVESEVLKRELTSLENSPELRRVDSSRKSRNFNKQPSTGDYYKHLGGGEPAGSNRKMAHSEEASLLSEESVQNGSAAESKPSGEVPPAENHPASTSQRRSSSSTGSTKSFNMMSPTGDNAELLAEIKAGKSLKPTPQSKGFTTVFSGCGQAGANVETPASSPSPTRSPTPPSTPEAASAPRPATSPEPVVNGSLPPLASSAPAGQVDLEGLIPTHDEQGRPIPEWKRQVMVRKLQMKIQEEEEQRRKEREEEARLATMPAWRRDILRKKMEEEREQKRKEQEKLRREEEEKEKEQSEKLRTLGYDETKLAPWQRQIILKKGDIAKH

Target ORF information:

RefSeq Version	XM_020800002.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps espin (ESPN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020800002.1

ORF Insert Sequence:

ATGAGCTATG TCACCCACGC CTTTTGCGCC GCAGACACGG ACCATTCTGG TGCCACCGTC 
TTACACCTGG CCGCCCGGTT TGGCCACCAC GAGGTGATCG ACTGGCTTCT GCGCTTCGGG 
GGCAGCGACC CGCTGGCGGC CACGGACACG GGGGCTCTCC CTGTTCACTA CGCTGCGGCC 
AAAGGAGACT TCCCTTCCTT GAGACTCCTC ATCGGGCATT GCCCCAGTTC GGTCACTGCC 
CAAACCAAGA ATGGTGCCAC GCCACTTTAC TTAGCCTGCC AGGAAGGCCA CCTGGAAATC 
ATCCAGTATC TGGTGAAGGA CTGCGGAGCG GATCCCCACG TACGCGCCAA TGACGGGATG 
ACCCCTCTCC ACGCTGCGGC CCAGATGGGA CACAACACCG TCATCGTTTG GCTGATGAGC 
TTCACCGACA TCAGCCTCTC CGAGCAGGAC GACGATGGGG CCACCGCCAT GCACTTTGCC 
GCCAGCCGGG GCCATGCCAA GGTGCTCAGC TGGCTGCTGC TGCACGGAGG GGAGATCACC 
ACAGACCACT GGGGAGGGAC CCCCTTGCAC GACGCGGCCG AGAACGGAGA GCTGGAGTGT 
TGCCAAATCT TAGTGGTGAA TGGCGTGAAC CTCGGCATCC GAGACCAGGA TGGCTACACG 
GCTGCCGACC TTGCAGACTA CAATGGGCAC GTGCACTGCG CCAAGTACCT GCGTACGGTG 
GAGAACATGA GCGTGGAACA CCGGGTCCTC TCGCGAGACC CGTCTACCGA CCTGGAGTTC 
AAGCAGCCCG ATTCGGGGAT GTCTTCGCCC AACACCACCG TGTCTGCCCC ACAGGCCCAC 
TTCAACCTGG GTTCGCCTAG CAGCACCCTC TCCAACTACG ACTCCTGCAA CTCCAGTCAG 
TCCAGCACAG GAGAGAAACG GAACAGCCAG GCCTCCCGTA AGCATCAGGC GGGGGGAGAC 
CCTCCCTCGG ACGGCGCTTC CCGAATTCCT GAAACGACCA TCACGGACAT GCAGACCTAT 
ATGGACATGC TGAATCCTCC CGAAGTCCAG CACACCGCGG AAATCTACGT CCAGGCTAAG 
AACAACCTCC GTCACGTGGA GAGCGAAGTG TTGAAGCGAG AGCTTACCTC TCTGGAGAAC 
AGCCCGGAGC TACGGCGCGT CGACTCCAGC CGGAAGTCGA GAAACTTCAA CAAGCAGCCC 
AGCACGGGCG ATTACTACAA ACATCTGGGC GGCGGCGAAC CAGCTGGGAG CAACCGGAAG 
ATGGCCCACA GCGAGGAGGC TTCGCTTCTG TCCGAGGAGA GTGTGCAAAA TGGGAGTGCG 
GCAGAGAGCA AGCCTTCCGG CGAGGTGCCT CCCGCCGAGA ACCACCCGGC TTCGACCAGC 
CAACGGCGCT CCTCCTCGTC GACTGGAAGC ACCAAATCCT TCAACATGAT GTCCCCGACC 
GGAGACAACG CAGAGCTGCT GGCTGAGATC AAGGCTGGGA AGAGCCTCAA GCCGACCCCA 
CAGAGCAAGG GCTTCACCAC CGTCTTCTCC GGCTGCGGCC AGGCCGGCGC CAACGTGGAG 
ACTCCGGCAT CCTCCCCTTC CCCAACCAGG AGCCCAACTC CTCCTTCTAC CCCCGAAGCA 
GCTTCTGCCC CCCGGCCTGC CACCTCGCCT GAACCGGTCG TGAACGGCTC CTTGCCGCCC 
CTCGCCTCCT CAGCCCCAGC CGGCCAAGTG GACCTCGAGG GCCTGATCCC CACCCACGAT 
GAGCAAGGCA GGCCCATCCC GGAGTGGAAG CGCCAGGTGA TGGTCCGGAA GCTCCAGATG 
AAGATCCAGG AGGAGGAGGA GCAGAGACGC AAGGAACGAG AAGAAGAGGC TCGCCTGGCT 
ACCATGCCCG CCTGGCGGAG AGACATCCTT CGCAAGAAGA TGGAAGAGGA AAGAGAGCAA 
AAACGAAAAG AGCAGGAGAA ACTGAGGCGG GAAGAGGAGG AGAAAGAGAA AGAGCAGTCG 
GAAAAGCTAA GGACTCTTGG TTACGATGAG ACTAAGCTGG CGCCCTGGCA AAGGCAGATC 
ATCCTCAAGA AAGGAGACAT CGCCAAGCAC TAG