ache cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	ache
Entrez Gene ID	103480137
Full Name	acetylcholinesterase (Cartwright blood group)
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008434980.2	XP_008433202.1	acetylcholinesterase

Pathways from BioSystems
Gene Ontology

The following ache gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ache cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo181860	XM_008434980.2 Latest version!	Poecilia reticulata acetylcholinesterase (Cartwright blood group) (ache), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ache ( XM_008434980.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008433202.1 Poecilia reticulata acetylcholinesterase (Cartwright blood group) (ache), mRNA.

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CloneID	OPo181860
Clone ID Related Accession (Same CDS sequence)	XM_008434980.2
Accession Version	XM_008434980.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	acetylcholinesterase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024348.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008434980.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTCT CCACTTATCT CGTTTTTTTG TCAGTCTTTC TCATTGCTCT CATCCCAGCC 
TGCTTTTCAC AAAATGATGG CGACCTTGTT AATACACATA GTGGTCTAGT TCAAGGTGCG 
CGCTTACCGG TGCCAGATGG GAGCTATGTC ACAGCTTTCC TCGGCATCCC TTTTGCTGAG 
CCACCACTGG GTAAGCGTCG CTTCCGTCCT CCTGAGCCGA AGCGAAGATG GACAGGGATT 
CTTGAGGCTA ATGCCTACCC CAACGCCTGC TACCAGTTTG TGGACACATC GTACCCGGGC 
TTCCAGGGCA CTGAGATGTG GAATCCTAAC AGAGAGATGA GCGAGGACTG CCTGTACCTT 
AACATCTGGG TGCCTGCCTC GCCCAGGCCC CACAATCTCT CGGTTATGGT GTGGATCTAT 
GGTGGAGGTT TCTACAGTGG CTCTTCTTCT CTGGATGTGT ATGATGGCCG TTACCTTGCT 
CACACTGAGA CGGTCATTGT GGTCTCCATG AATTACCGTA TTGGAGCCTT TGGTTTCCTC 
GCTCTTCATG GCTCTTCAGA GGCTCCTGGA AATGTCGGCC TGCTGGACCA GAGGTTGGCA 
TTGAAGTGGG TGCAAGAAAA TATCCATTCC TTCGGTGGAA ACCCCAAACA GGTGACTATC 
TTCGGTGAGA GTGCTGGCGG TGCTTCAGTC GGGTTCCACC TGTTGTCTCC AGACAGCAGA 
CCTTTTTTCA CAAGGGCCAT CCTTCAGAGC GGGGTCCCCA ACGGTCCGTG GGCCTCTGTC 
AGCCCTGCTG AGGCCAGAAG ACGTGCCACG CTTCTGGCAA AGCTTGTTAA CTGTAATGGA 
GGCAACGACA CTGAGCTGGT GGACTGTCTG CGCAATAAAA CTCCACAGGA GCTTATCGAC 
CAAGAATGGC TGGTTCTGCC ATGGTCAGCT CTGTTCCGGT TCTCCTTTGT ACCAGTTGTG 
GATGGTGATT TTCTTCCTGA TACTCCAGAA GCAATGATTA ACTCAGGCAA TTTTAAAGAC 
ACTCAGATTC TTCTTGGTGT CAACCAAGAC GAGGGTTCTT ACTTTCTGCT GTATGGAGCG 
CCGGGATTCA GCAAGGACAA CGAAAGTCTA ATTTCCAAAG AAGACTTCCT GGAAGGTGTA 
CGATTGAGCG TACCGCACGC AAACGATATC GGCTTGGAGG CGGTGGTGCT GCAGTACACC 
GACTGGATGG ATGAGAATAA CGGAGTGAAA AACCGCGATG CCATGGACGA CATTGTTGGC 
GATCACAATG TAATTTGCCC ACTGGCACAT TTTGCTCGTT CTTATGCACA GCACCACACT 
CTAAAGGCGA ACCTGGGCAT GATGAACAGT GCAGGAAACT CACAAGGCGG CGTGTACCTC 
TACCTGTTTG ACCACCGGGC TTCTAATTTA GCCTGGCCGG AGTGGATGGG AGTCATCCAT 
GGCTATGAAA TCGAGTTTGT CTTTGGGTTG CCACTGGAGA AGAGACTCAA TTACACCACC 
GAAGAGAAGA ATCTGAGTCG GCGCATGATG AAATACTGGG CAAACTTTGC ACGGACAGGG 
AATCCTAACG ACGGGCTGCT GGACACTAGG AAACGCTGGC CTTTGTTTAC GGCGAATGAG 
CAGAAACATG TCGCACTCAA CACCGACCCT CTTAAAATCC ACAAAGGTTT GCGCAACCAG 
ATGTGTGCTT TCTGGAACCG CTTTCTGCCA CGCCTTTTGA ACATCACAGG CAACATCGAC 
GAGGCAGAGC GTCAGTGGAA GGTGGAGTTT CACCGCTGGT CCTCCTACAT GATGCACTGG 
AAGAGTCAGT TTGATCACTA CAGCAAGCAG GAGCGCTGCA CCGACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008433202.1
CDS	361..2211
Translation	MQFSTYLVFLSVFLIALIPACFSQNDGDLVNTHSGLVQGARLPVPDGSYVTAFLGIPFAEPPLGKRRFRPPEPKRRWTGILEANAYPNACYQFVDTSYPGFQGTEMWNPNREMSEDCLYLNIWVPASPRPHNLSVMVWIYGGGFYSGSSSLDVYDGRYLAHTETVIVVSMNYRIGAFGFLALHGSSEAPGNVGLLDQRLALKWVQENIHSFGGNPKQVTIFGESAGGASVGFHLLSPDSRPFFTRAILQSGVPNGPWASVSPAEARRRATLLAKLVNCNGGNDTELVDCLRNKTPQELIDQEWLVLPWSALFRFSFVPVVDGDFLPDTPEAMINSGNFKDTQILLGVNQDEGSYFLLYGAPGFSKDNESLISKEDFLEGVRLSVPHANDIGLEAVVLQYTDWMDENNGVKNRDAMDDIVGDHNVICPLAHFARSYAQHHTLKANLGMMNSAGNSQGGVYLYLFDHRASNLAWPEWMGVIHGYEIEFVFGLPLEKRLNYTTEEKNLSRRMMKYWANFARTGNPNDGLLDTRKRWPLFTANEQKHVALNTDPLKIHKGLRNQMCAFWNRFLPRLLNITGNIDEAERQWKVEFHRWSSYMMHWKSQFDHYSKQERCTDL

Target ORF information:

RefSeq Version	XM_008434980.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata acetylcholinesterase (Cartwright blood group) (ache), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008434980.2

ORF Insert Sequence:

ATGCAGTTCT CCACTTATCT CGTTTTTTTG TCAGTCTTTC TCATTGCTCT CATCCCAGCC 
TGCTTTTCAC AAAATGATGG CGACCTTGTT AATACACATA GTGGTCTAGT TCAAGGTGCG 
CGCTTACCGG TGCCAGATGG GAGCTATGTC ACAGCTTTCC TCGGCATCCC TTTTGCTGAG 
CCACCACTGG GTAAGCGTCG CTTCCGTCCT CCTGAGCCGA AGCGAAGATG GACAGGGATT 
CTTGAGGCTA ATGCCTACCC CAACGCCTGC TACCAGTTTG TGGACACATC GTACCCGGGC 
TTCCAGGGCA CTGAGATGTG GAATCCTAAC AGAGAGATGA GCGAGGACTG CCTGTACCTT 
AACATCTGGG TGCCTGCCTC GCCCAGGCCC CACAATCTCT CGGTTATGGT GTGGATCTAT 
GGTGGAGGTT TCTACAGTGG CTCTTCTTCT CTGGATGTGT ATGATGGCCG TTACCTTGCT 
CACACTGAGA CGGTCATTGT GGTCTCCATG AATTACCGTA TTGGAGCCTT TGGTTTCCTC 
GCTCTTCATG GCTCTTCAGA GGCTCCTGGA AATGTCGGCC TGCTGGACCA GAGGTTGGCA 
TTGAAGTGGG TGCAAGAAAA TATCCATTCC TTCGGTGGAA ACCCCAAACA GGTGACTATC 
TTCGGTGAGA GTGCTGGCGG TGCTTCAGTC GGGTTCCACC TGTTGTCTCC AGACAGCAGA 
CCTTTTTTCA CAAGGGCCAT CCTTCAGAGC GGGGTCCCCA ACGGTCCGTG GGCCTCTGTC 
AGCCCTGCTG AGGCCAGAAG ACGTGCCACG CTTCTGGCAA AGCTTGTTAA CTGTAATGGA 
GGCAACGACA CTGAGCTGGT GGACTGTCTG CGCAATAAAA CTCCACAGGA GCTTATCGAC 
CAAGAATGGC TGGTTCTGCC ATGGTCAGCT CTGTTCCGGT TCTCCTTTGT ACCAGTTGTG 
GATGGTGATT TTCTTCCTGA TACTCCAGAA GCAATGATTA ACTCAGGCAA TTTTAAAGAC 
ACTCAGATTC TTCTTGGTGT CAACCAAGAC GAGGGTTCTT ACTTTCTGCT GTATGGAGCG 
CCGGGATTCA GCAAGGACAA CGAAAGTCTA ATTTCCAAAG AAGACTTCCT GGAAGGTGTA 
CGATTGAGCG TACCGCACGC AAACGATATC GGCTTGGAGG CGGTGGTGCT GCAGTACACC 
GACTGGATGG ATGAGAATAA CGGAGTGAAA AACCGCGATG CCATGGACGA CATTGTTGGC 
GATCACAATG TAATTTGCCC ACTGGCACAT TTTGCTCGTT CTTATGCACA GCACCACACT 
CTAAAGGCGA ACCTGGGCAT GATGAACAGT GCAGGAAACT CACAAGGCGG CGTGTACCTC 
TACCTGTTTG ACCACCGGGC TTCTAATTTA GCCTGGCCGG AGTGGATGGG AGTCATCCAT 
GGCTATGAAA TCGAGTTTGT CTTTGGGTTG CCACTGGAGA AGAGACTCAA TTACACCACC 
GAAGAGAAGA ATCTGAGTCG GCGCATGATG AAATACTGGG CAAACTTTGC ACGGACAGGG 
AATCCTAACG ACGGGCTGCT GGACACTAGG AAACGCTGGC CTTTGTTTAC GGCGAATGAG 
CAGAAACATG TCGCACTCAA CACCGACCCT CTTAAAATCC ACAAAGGTTT GCGCAACCAG 
ATGTGTGCTT TCTGGAACCG CTTTCTGCCA CGCCTTTTGA ACATCACAGG CAACATCGAC 
GAGGCAGAGC GTCAGTGGAA GGTGGAGTTT CACCGCTGGT CCTCCTACAT GATGCACTGG 
AAGAGTCAGT TTGATCACTA CAGCAAGCAG GAGCGCTGCA CCGACCTCTG A