lrrc8d cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	lrrc8d
Entrez Gene ID	103479113
Full Name	leucine rich repeat containing 8 VRAC subunit D
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008433355.2	XP_008431577.1	volume-regulated anion channel subunit LRRC8D

Pathways from BioSystems
Gene Ontology

The following lrrc8d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lrrc8d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo179989	XM_008433355.2 Latest version!	Poecilia reticulata leucine rich repeat containing 8 family member D (lrrc8d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lrrc8d ( XM_008433355.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008431577.1 Poecilia reticulata leucine rich repeat containing 8 family member D (lrrc8d), mRNA.

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CloneID	OPo179989
Clone ID Related Accession (Same CDS sequence)	XM_008433355.2
Accession Version	XM_008433355.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	volume-regulated anion channel subunit LRRC8D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024347.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008433355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTACAC TCACTGAGGT TGCATCCCTG AACGACATCC AGCCGACGTA TCGCATCTTA 
AAGCCATGGT GGGACGTCTT CATGGACTAT CTGGGCCTGG TCATGCTCAT GTTGGCCATA 
TTTGCAATGA CCATGCAAAT CACCAAGGAC CATGTCGCTT GCCTTCCTTG TCCTGAAGAA 
ACGGAAGCTA ATCTGTACCA GACCCAAAAA GGATCCTCTC CAACACCCGC CGGAGCCCCT 
GTTACACCTA CAGTCCCTTC AGGTGCTACG GCGCAGTTCA ACAAAGCCAT CACTGGTATT 
CTCATGACAC ATCGGCCTGG AACAGCAGAA CAGAAAACAG ATCCCCAACC TGAACCGAGA 
GGGGTTAAAA CCAACCTGGA CTTTCAACAA TATGTCTTTA TCAACCAAAT GTGTTACCAT 
GATGCCTTAC CCTGGTCTTC CAAATACTTT CCATACCTTA CACTTATTCA CACCCTTATT 
CTCATGGTCA GTAGCAATTT CTGGTTTAAA TACCCCAAGA CAAGTTCAAA GATTGAGCAT 
TTTGTCTCCA TTCTGGGTCG GTGTTTTGAG TCGCCTTGGA CAACGAAGGC TTTATCTGAA 
ACGGCGTGTG AGGACTCTGA GGAGAACAAA CAGAGGTTGA CGGGTAGTTC CTCAGGACCA 
AAGCAGGTGT CTTTAGAGGG GAAGGATGAA AGTCCCGACA CCAACCCATC CACACCAATG 
CTTGGGGTCA AAGTCTCTGC AGATAAACCC ATTGCAGAGG TCCCAAGCAG CATGACCATC 
TTGGATAAAA AGGACGGAGA GCAGGCCAAA GCCCTGTTTG AGAAGGTGCG AAAGTTCCGA 
GCCCACGTGG AAGACAGTGA TTTCATTTAC AGGCTCTATG TCGCTCAAAC CTCTGTCAAA 
ACTGTTAAGT TTATTTTGAT CCTGTCCTAT ACTTCCACCT TGGTTAGAAT AGAATTCAAG 
CATTATTGTG AACCAGACAT AGAACAGCTA ACAGGCTATA AAAAATTCTA CTGTACACAC 
AACATGGCTT TCATGCTGCA CAAGTTGCTC TTTACCTACA TAGTGTTGAT CATAATCTAT 
GGAATGACAT GCCTATACTC CCTTTTCTGG GTGTTCCGAC GCCCTCTGAA AGAGTATTCC 
TTTGAGAAGG TGAGGGAAGA GAGCAGCTTC AGTGACATTC CGGATGTCAA GAACGACTTT 
GCGTTTCTAC TGCACATGGT TGACCAGTAC GATCAGCTTT ACTCCAAGCG CTTTGGCGTC 
TTCCTGTCGG AGGTCAGTGA AAACAAGTTG AGGGAGATCA GCCTCAATCA TGAGTGGACG 
TTTGAAAAAC TAAAGCAGCA TGTCACGCGC AATGCACACG ACCAACAGGA GCTGCACCTC 
TTCATGCTGT CCGGCCTGCC GAACGCTGTG TTTGACCTGA CAGACCTGGA AGTGCTGAAG 
CTGGAGTTGA TTCCCGAGGT GAGATTTTCC GCTAAGGTTT CCCAGATGAC CAGCATGCAG 
GAGCTGCATC TGTGCCACTG TCCGGCCAAA GTGGAGCAAA CCGGTTTTGC CTTCCTACGG 
GATCACCTGC GCCGCCTGCA CGTCAAGTTC ACGGACGTCG CCGAGATCCC GCCTTGGGTC 
TACTTGCTGC GGAGTCTGAA AGAGCTGTAC CTAATGGGGA ACTTGAACTC AGAAAACAAC 
AAAATGATCG GCCTGGAGTC CATGAGGGAT TTGAGGCATC TGAAGACGCT ATGCCTGAAG 
AGCAATCTCA CGAAGATGCC GACAAACATC ACAGAGCTCT CCCCGCATCT CCTCCGACTG 
GTGGTGCACA ACGATGGGAC GAAACTGCTG GTGCTGAACA GTCTGAAGAA AATGACGAGC 
CTCATCGAGC TAGAGCTGTA CAACTGTGAA CTGGAAAGGA TCCCTCATGC CATCTTTAGC 
CTGACCAACC TGCAGGAACT CGACTTGAAG TCCAACAACA TCAGAACTAT TGAAGAGATC 
ATCAGCTTCC AGCATCTCAG GAGGCTGACG TGCCTAAAGC TCTGGCACAA CAAAATCATC 
ACCATCCCCG CATCCATCGG TCACGTCAAG TCCCTGGAAT CTCTCCACCT TTCTCACAAT 
AAACTGGAGT CCCTTCCTTC AGCCTTGTTT ACTCTTCCCA AGCTGCGACA CTTGGACGTG 
GCCCACAACT CCATCACAGT GCTTCCACCT GACGTGGGCC TCCTCAACAA CCTCCAGCAT 
CTGGCCATTA ACTCGAACAA GCTGGAAGCC CTGCCCAAAC CTCTCTTCAG ATGCACCAAG 
CTGAAGGTGC TTTGTCTAGG CCACAACGCG CTCACCAGTC TGCCAGAGAC CGTGGGTCAA 
CTGGTGCAGC TCACGCAGCT GGAGCTTAGA GGGAACTGCC TCGACAGACT GCCCGTCCAG 
TTGGGAAGCT GCCGGCTGCT GCAGAAGAAT GGCCTCATCG TGGAGGACCA TCTTTTTGAC 
ACCCTGCCGG TGGAAGTTAA AGAGAGCATC AGCAGAGAAA CAAATGTTTC CTTTAGTGGC 
TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008431577.1
CDS	326..2851
Translation	MFTLTEVASLNDIQPTYRILKPWWDVFMDYLGLVMLMLAIFAMTMQITKDHVACLPCPEETEANLYQTQKGSSPTPAGAPVTPTVPSGATAQFNKAITGILMTHRPGTAEQKTDPQPEPRGVKTNLDFQQYVFINQMCYHDALPWSSKYFPYLTLIHTLILMVSSNFWFKYPKTSSKIEHFVSILGRCFESPWTTKALSETACEDSEENKQRLTGSSSGPKQVSLEGKDESPDTNPSTPMLGVKVSADKPIAEVPSSMTILDKKDGEQAKALFEKVRKFRAHVEDSDFIYRLYVAQTSVKTVKFILILSYTSTLVRIEFKHYCEPDIEQLTGYKKFYCTHNMAFMLHKLLFTYIVLIIIYGMTCLYSLFWVFRRPLKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLKQHVTRNAHDQQELHLFMLSGLPNAVFDLTDLEVLKLELIPEVRFSAKVSQMTSMQELHLCHCPAKVEQTGFAFLRDHLRRLHVKFTDVAEIPPWVYLLRSLKELYLMGNLNSENNKMIGLESMRDLRHLKTLCLKSNLTKMPTNITELSPHLLRLVVHNDGTKLLVLNSLKKMTSLIELELYNCELERIPHAIFSLTNLQELDLKSNNIRTIEEIISFQHLRRLTCLKLWHNKIITIPASIGHVKSLESLHLSHNKLESLPSALFTLPKLRHLDVAHNSITVLPPDVGLLNNLQHLAINSNKLEALPKPLFRCTKLKVLCLGHNALTSLPETVGQLVQLTQLELRGNCLDRLPVQLGSCRLLQKNGLIVEDHLFDTLPVEVKESISRETNVSFSGL

Target ORF information:

RefSeq Version	XM_008433355.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata leucine rich repeat containing 8 family member D (lrrc8d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008433355.2

ORF Insert Sequence:

ATGTTTACAC TCACTGAGGT TGCATCCCTG AACGACATCC AGCCGACGTA TCGCATCTTA 
AAGCCATGGT GGGACGTCTT CATGGACTAT CTGGGCCTGG TCATGCTCAT GTTGGCCATA 
TTTGCAATGA CCATGCAAAT CACCAAGGAC CATGTCGCTT GCCTTCCTTG TCCTGAAGAA 
ACGGAAGCTA ATCTGTACCA GACCCAAAAA GGATCCTCTC CAACACCCGC CGGAGCCCCT 
GTTACACCTA CAGTCCCTTC AGGTGCTACG GCGCAGTTCA ACAAAGCCAT CACTGGTATT 
CTCATGACAC ATCGGCCTGG AACAGCAGAA CAGAAAACAG ATCCCCAACC TGAACCGAGA 
GGGGTTAAAA CCAACCTGGA CTTTCAACAA TATGTCTTTA TCAACCAAAT GTGTTACCAT 
GATGCCTTAC CCTGGTCTTC CAAATACTTT CCATACCTTA CACTTATTCA CACCCTTATT 
CTCATGGTCA GTAGCAATTT CTGGTTTAAA TACCCCAAGA CAAGTTCAAA GATTGAGCAT 
TTTGTCTCCA TTCTGGGTCG GTGTTTTGAG TCGCCTTGGA CAACGAAGGC TTTATCTGAA 
ACGGCGTGTG AGGACTCTGA GGAGAACAAA CAGAGGTTGA CGGGTAGTTC CTCAGGACCA 
AAGCAGGTGT CTTTAGAGGG GAAGGATGAA AGTCCCGACA CCAACCCATC CACACCAATG 
CTTGGGGTCA AAGTCTCTGC AGATAAACCC ATTGCAGAGG TCCCAAGCAG CATGACCATC 
TTGGATAAAA AGGACGGAGA GCAGGCCAAA GCCCTGTTTG AGAAGGTGCG AAAGTTCCGA 
GCCCACGTGG AAGACAGTGA TTTCATTTAC AGGCTCTATG TCGCTCAAAC CTCTGTCAAA 
ACTGTTAAGT TTATTTTGAT CCTGTCCTAT ACTTCCACCT TGGTTAGAAT AGAATTCAAG 
CATTATTGTG AACCAGACAT AGAACAGCTA ACAGGCTATA AAAAATTCTA CTGTACACAC 
AACATGGCTT TCATGCTGCA CAAGTTGCTC TTTACCTACA TAGTGTTGAT CATAATCTAT 
GGAATGACAT GCCTATACTC CCTTTTCTGG GTGTTCCGAC GCCCTCTGAA AGAGTATTCC 
TTTGAGAAGG TGAGGGAAGA GAGCAGCTTC AGTGACATTC CGGATGTCAA GAACGACTTT 
GCGTTTCTAC TGCACATGGT TGACCAGTAC GATCAGCTTT ACTCCAAGCG CTTTGGCGTC 
TTCCTGTCGG AGGTCAGTGA AAACAAGTTG AGGGAGATCA GCCTCAATCA TGAGTGGACG 
TTTGAAAAAC TAAAGCAGCA TGTCACGCGC AATGCACACG ACCAACAGGA GCTGCACCTC 
TTCATGCTGT CCGGCCTGCC GAACGCTGTG TTTGACCTGA CAGACCTGGA AGTGCTGAAG 
CTGGAGTTGA TTCCCGAGGT GAGATTTTCC GCTAAGGTTT CCCAGATGAC CAGCATGCAG 
GAGCTGCATC TGTGCCACTG TCCGGCCAAA GTGGAGCAAA CCGGTTTTGC CTTCCTACGG 
GATCACCTGC GCCGCCTGCA CGTCAAGTTC ACGGACGTCG CCGAGATCCC GCCTTGGGTC 
TACTTGCTGC GGAGTCTGAA AGAGCTGTAC CTAATGGGGA ACTTGAACTC AGAAAACAAC 
AAAATGATCG GCCTGGAGTC CATGAGGGAT TTGAGGCATC TGAAGACGCT ATGCCTGAAG 
AGCAATCTCA CGAAGATGCC GACAAACATC ACAGAGCTCT CCCCGCATCT CCTCCGACTG 
GTGGTGCACA ACGATGGGAC GAAACTGCTG GTGCTGAACA GTCTGAAGAA AATGACGAGC 
CTCATCGAGC TAGAGCTGTA CAACTGTGAA CTGGAAAGGA TCCCTCATGC CATCTTTAGC 
CTGACCAACC TGCAGGAACT CGACTTGAAG TCCAACAACA TCAGAACTAT TGAAGAGATC 
ATCAGCTTCC AGCATCTCAG GAGGCTGACG TGCCTAAAGC TCTGGCACAA CAAAATCATC 
ACCATCCCCG CATCCATCGG TCACGTCAAG TCCCTGGAAT CTCTCCACCT TTCTCACAAT 
AAACTGGAGT CCCTTCCTTC AGCCTTGTTT ACTCTTCCCA AGCTGCGACA CTTGGACGTG 
GCCCACAACT CCATCACAGT GCTTCCACCT GACGTGGGCC TCCTCAACAA CCTCCAGCAT 
CTGGCCATTA ACTCGAACAA GCTGGAAGCC CTGCCCAAAC CTCTCTTCAG ATGCACCAAG 
CTGAAGGTGC TTTGTCTAGG CCACAACGCG CTCACCAGTC TGCCAGAGAC CGTGGGTCAA 
CTGGTGCAGC TCACGCAGCT GGAGCTTAGA GGGAACTGCC TCGACAGACT GCCCGTCCAG 
TTGGGAAGCT GCCGGCTGCT GCAGAAGAAT GGCCTCATCG TGGAGGACCA TCTTTTTGAC 
ACCCTGCCGG TGGAAGTTAA AGAGAGCATC AGCAGAGAAA CAAATGTTTC CTTTAGTGGC 
TTATAA