LOC103473214 cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103473214
Entrez Gene ID	103473214
Full Name	interleukin-1 beta-like
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008423254.2	XP_008421476.1	interleukin-1 beta-like isoform X2
XM_008423252.2	XP_008421474.1	interleukin-1 beta-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC103473214 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103473214 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo172025	XM_008423252.2 Latest version!	Poecilia reticulata interleukin-1 beta-like (LOC103473214), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OPo172026	XM_008423254.2 Latest version!	Poecilia reticulata interleukin-1 beta-like (LOC103473214), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103473214 ( XM_008423252.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008421474.1 Poecilia reticulata interleukin-1 beta-like (LOC103473214), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo172025
Clone ID Related Accession (Same CDS sequence)	XM_008423252.2
Accession Version	XM_008423252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	interleukin-1 beta-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024342.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008423252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACT TTGATTTAGC TGATGCGCTG GACTGCTCAC CTGAAATCAG ACGTCCCCCC 
AGTGGTACAG AGCAGGATGT GGAGCGTGAA GTGAAGCTCA GCGAAGACCT GAACCTGATG 
GTCACCAGCA ACAGGATGAC CATGAAGAGT GTAGCCAACC TGGTGCTGGC CGTGAACAAG 
CTGAGCAAGT CCCTGTCTCT GTGTGGCAGA GACCTCAGCG ATGTGGAGCT CTGCGGCGCC 
ATCATGGACT GCGTGGTGGA AGAAACCATT TTCCAGGAAG GATGGAGCAA CTCCTCAGGA 
ACGAGGAAGC CAGTCTTCCA GCGGTGCAGC AGTGTTCATA AGTTCACAGT GAGCGACGAG 
TACCAGAAAG ATTTCATCTT GACAACAGGA GAGATGAAGC TGCAGGCCAT CACTCTGAGA 
GCCGGAAACG GTGATCGCAA AGTGAACTTT GAGATGTGCC ACTACAGGCC GCCCTGCAGC 
TCCAGCATAC GTTCTTTGAC GGTTCTGTTA TCCGTCTCTG AGAACTTGCA CATTTCTTGC 
TCCATGGAAG ATGGGAAAGT TTCACTGGAA TTGGAGCAAT ACAGACATGA GTCTCTGACA 
GAGATCAACC AGGAAGACGG CTTGGACCGC TTTCTCTTCA TCAAGAACAC CCCCCCTGGT 
GGCTCCCTGG TCAGCTTTGA GTCGGTGAAG TTCCGCGGTT GGTTCATCAG CACTTCCTCT 
GTGGAAATCG ACCACTCAGT GGAGATGTGC CAGGCGGCCA CCGCCTCCAG GGTCACCTGC 
TTCAAAGTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008421474.1
CDS	132..926
Translation	MSDFDLADALDCSPEIRRPPSGTEQDVEREVKLSEDLNLMVTSNRMTMKSVANLVLAVNKLSKSLSLCGRDLSDVELCGAIMDCVVEETIFQEGWSNSSGTRKPVFQRCSSVHKFTVSDEYQKDFILTTGEMKLQAITLRAGNGDRKVNFEMCHYRPPCSSSIRSLTVLLSVSENLHISCSMEDGKVSLELEQYRHESLTEINQEDGLDRFLFIKNTPPGGSLVSFESVKFRGWFISTSSVEIDHSVEMCQAATASRVTCFKVF

Target ORF information:

RefSeq Version	XM_008423252.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata interleukin-1 beta-like (LOC103473214), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008423252.2

ORF Insert Sequence:

ATGTCGGACT TTGATTTAGC TGATGCGCTG GACTGCTCAC CTGAAATCAG ACGTCCCCCC 
AGTGGTACAG AGCAGGATGT GGAGCGTGAA GTGAAGCTCA GCGAAGACCT GAACCTGATG 
GTCACCAGCA ACAGGATGAC CATGAAGAGT GTAGCCAACC TGGTGCTGGC CGTGAACAAG 
CTGAGCAAGT CCCTGTCTCT GTGTGGCAGA GACCTCAGCG ATGTGGAGCT CTGCGGCGCC 
ATCATGGACT GCGTGGTGGA AGAAACCATT TTCCAGGAAG GATGGAGCAA CTCCTCAGGA 
ACGAGGAAGC CAGTCTTCCA GCGGTGCAGC AGTGTTCATA AGTTCACAGT GAGCGACGAG 
TACCAGAAAG ATTTCATCTT GACAACAGGA GAGATGAAGC TGCAGGCCAT CACTCTGAGA 
GCCGGAAACG GTGATCGCAA AGTGAACTTT GAGATGTGCC ACTACAGGCC GCCCTGCAGC 
TCCAGCATAC GTTCTTTGAC GGTTCTGTTA TCCGTCTCTG AGAACTTGCA CATTTCTTGC 
TCCATGGAAG ATGGGAAAGT TTCACTGGAA TTGGAGCAAT ACAGACATGA GTCTCTGACA 
GAGATCAACC AGGAAGACGG CTTGGACCGC TTTCTCTTCA TCAAGAACAC CCCCCCTGGT 
GGCTCCCTGG TCAGCTTTGA GTCGGTGAAG TTCCGCGGTT GGTTCATCAG CACTTCCTCT 
GTGGAAATCG ACCACTCAGT GGAGATGTGC CAGGCGGCCA CCGCCTCCAG GGTCACCTGC 
TTCAAAGTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103473214 ( XM_008423254.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008421476.1 Poecilia reticulata interleukin-1 beta-like (LOC103473214), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo172026
Clone ID Related Accession (Same CDS sequence)	XM_008423254.2
Accession Version	XM_008423254.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	interleukin-1 beta-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024342.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008423254.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACT TTGATTTAGC TGATGCGCTG GACTGCTCAC CTGAAATCAG ACGTCCCCCC 
AGTGGTACAG AGGATGTGGA GCGTGAAGTG AAGCTCAGCG AAGACCTGAA CCTGATGGTC 
ACCAGCAACA GGATGACCAT GAAGAGTGTA GCCAACCTGG TGCTGGCCGT GAACAAGCTG 
AGCAAGTCCC TGTCTCTGTG TGGCAGAGAC CTCAGCGATG TGGAGCTCTG CGGCGCCATC 
ATGGACTGCG TGGTGGAAGA AACCATTTTC CAGGAAGGAT GGAGCAACTC CTCAGGAACG 
AGGAAGCCAG TCTTCCAGCG GTGCAGCAGT GTTCATAAGT TCACAGTGAG CGACGAGTAC 
CAGAAAGATT TCATCTTGAC AACAGGAGAG ATGAAGCTGC AGGCCATCAC TCTGAGAGCC 
GGAAACGGTG ATCGCAAAGT GAACTTTGAG ATGTGCCACT ACAGGCCGCC CTGCAGCTCC 
AGCATACGTT CTTTGACGGT TCTGTTATCC GTCTCTGAGA ACTTGCACAT TTCTTGCTCC 
ATGGAAGATG GGAAAGTTTC ACTGGAATTG GAGCAATACA GACATGAGTC TCTGACAGAG 
ATCAACCAGG AAGACGGCTT GGACCGCTTT CTCTTCATCA AGAACACCCC CCCTGGTGGC 
TCCCTGGTCA GCTTTGAGTC GGTGAAGTTC CGCGGTTGGT TCATCAGCAC TTCCTCTGTG 
GAAATCGACC ACTCAGTGGA GATGTGCCAG GCGGCCACCG CCTCCAGGGT CACCTGCTTC 
AAAGTCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008421476.1
CDS	132..923
Translation	MSDFDLADALDCSPEIRRPPSGTEDVEREVKLSEDLNLMVTSNRMTMKSVANLVLAVNKLSKSLSLCGRDLSDVELCGAIMDCVVEETIFQEGWSNSSGTRKPVFQRCSSVHKFTVSDEYQKDFILTTGEMKLQAITLRAGNGDRKVNFEMCHYRPPCSSSIRSLTVLLSVSENLHISCSMEDGKVSLELEQYRHESLTEINQEDGLDRFLFIKNTPPGGSLVSFESVKFRGWFISTSSVEIDHSVEMCQAATASRVTCFKVF

Target ORF information:

RefSeq Version	XM_008423254.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata interleukin-1 beta-like (LOC103473214), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008423254.2

ORF Insert Sequence:

ATGTCGGACT TTGATTTAGC TGATGCGCTG GACTGCTCAC CTGAAATCAG ACGTCCCCCC 
AGTGGTACAG AGGATGTGGA GCGTGAAGTG AAGCTCAGCG AAGACCTGAA CCTGATGGTC 
ACCAGCAACA GGATGACCAT GAAGAGTGTA GCCAACCTGG TGCTGGCCGT GAACAAGCTG 
AGCAAGTCCC TGTCTCTGTG TGGCAGAGAC CTCAGCGATG TGGAGCTCTG CGGCGCCATC 
ATGGACTGCG TGGTGGAAGA AACCATTTTC CAGGAAGGAT GGAGCAACTC CTCAGGAACG 
AGGAAGCCAG TCTTCCAGCG GTGCAGCAGT GTTCATAAGT TCACAGTGAG CGACGAGTAC 
CAGAAAGATT TCATCTTGAC AACAGGAGAG ATGAAGCTGC AGGCCATCAC TCTGAGAGCC 
GGAAACGGTG ATCGCAAAGT GAACTTTGAG ATGTGCCACT ACAGGCCGCC CTGCAGCTCC 
AGCATACGTT CTTTGACGGT TCTGTTATCC GTCTCTGAGA ACTTGCACAT TTCTTGCTCC 
ATGGAAGATG GGAAAGTTTC ACTGGAATTG GAGCAATACA GACATGAGTC TCTGACAGAG 
ATCAACCAGG AAGACGGCTT GGACCGCTTT CTCTTCATCA AGAACACCCC CCCTGGTGGC 
TCCCTGGTCA GCTTTGAGTC GGTGAAGTTC CGCGGTTGGT TCATCAGCAC TTCCTCTGTG 
GAAATCGACC ACTCAGTGGA GATGTGCCAG GCGGCCACCG CCTCCAGGGT CACCTGCTTC 
AAAGTCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.