twf2 cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	twf2
Entrez Gene ID	103464500
Full Name	twinfilin actin binding protein 2
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017304979.1	XP_017160468.1	twinfilin-2 isoform X2
XM_008408646.2	XP_008406868.2	twinfilin-2 isoform X1

Pathways from BioSystems
Gene Ontology

The following twf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the twf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo195280	XM_008408646.2 Latest version!	Poecilia reticulata twinfilin actin binding protein 2 (twf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OPo195281	XM_017304979.1 Latest version!	Poecilia reticulata twinfilin actin binding protein 2 (twf2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

twf2 ( XM_008408646.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008406868.2 Poecilia reticulata twinfilin actin binding protein 2 (twf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo195280
Clone ID Related Accession (Same CDS sequence)	XM_008408646.2
Accession Version	XM_008408646.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	twinfilin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008408646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTTG CGTTGGTGGT GACGCCGGAA CTGCGGCAGT TCCTGGCTAG GGCAAGAGGC 
GGAGCGGCGC GCCTCATCAA AGTCTGCATC CAGGACGAGC AGCTGGTGCT GGGAGCCTAC 
AGGGAGCCAC AGCAACACTG GGACCAGGAC TATGACCACT TCCTGCTTCC TCTCCTGGAC 
GACCAGGAGC CCTGCTACAT CCTGTACCGT CTCGACTCCC AGAATGCTTT GGGCTACGAG 
TGGATTTTCA TCTCCTGGTC TCCTGACCAG TCTCCAGTGA AGCAGAAGAT GTTGTACGCC 
GCCACTCGTG CCACAGTGAA GAAAGAGTTT GGCGGCGGCC ACGTGAAGTA CGAGTTATTT 
GGCACCACGG AGGAGGATGT CTGCTTGCAG GGCTACCAGC ATTACGTGTC GTCCTGCTCG 
GGTCCAGCTC CGCTCACGCT AGCTGAGCAG GAGCTGCAGA GGATCAAAAT CACAGAGGGC 
CAGGTAAAAC AGGTGAAGAC AGAAATCAGC GTGGAGAGCA AACACCAGAC ACTTCAGGGC 
CTCGCTTTTC CTCTGCAGGA GTCGGCCAAA AGAAGCCTAC ACCTGCTGGC CCAGAGGCGC 
ATTAACTATG TGCAGCTGAG GCTGGATGTG GAAAAAGAGA CGATAGAGCT GGTCCACGCC 
AACCCCACAG AAACTCGTGA TTTACCCCGC CGGGTTCCCA AAGACACTCC CAGATACCAC 
TTCTTCCTTT ACAAGCACTC CCATGAAGGA GATTACCTGG AATCTGTTGT CTTCATATAC 
TCCATGCCAG GATACAGCTG CAGCATTAAG GAGCGGATGC TGTATTCCAG CTGCAAAAGT 
CGACTACTGG AGGATGTGGA GAGGGATTAC CGCTTGGAAA TAGCCAAAAA GTTGGAGATT 
GACGATGGAG ACGACCTGAC GGAGGAATTT CTGTACGACG AGGTCCATCC TAAGCAGTAC 
GCTCACAAGC AGGCCTTTGC AAAGCCCCGA GGCCCAGCAG GAAAACGGGG ACATAAGCGT 
CTTATTAAGG GTGCAGGAGA GGCCATTCAG GACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008406868.2
CDS	321..1379
Translation	MFLALVVTPELRQFLARARGGAARLIKVCIQDEQLVLGAYREPQQHWDQDYDHFLLPLLDDQEPCYILYRLDSQNALGYEWIFISWSPDQSPVKQKMLYAATRATVKKEFGGGHVKYELFGTTEEDVCLQGYQHYVSSCSGPAPLTLAEQELQRIKITEGQVKQVKTEISVESKHQTLQGLAFPLQESAKRSLHLLAQRRINYVQLRLDVEKETIELVHANPTETRDLPRRVPKDTPRYHFFLYKHSHEGDYLESVVFIYSMPGYSCSIKERMLYSSCKSRLLEDVERDYRLEIAKKLEIDDGDDLTEEFLYDEVHPKQYAHKQAFAKPRGPAGKRGHKRLIKGAGEAIQDS

Target ORF information:

RefSeq Version	XM_008408646.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata twinfilin actin binding protein 2 (twf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008408646.2

ORF Insert Sequence:

ATGTTTCTTG CGTTGGTGGT GACGCCGGAA CTGCGGCAGT TCCTGGCTAG GGCAAGAGGC 
GGAGCGGCGC GCCTCATCAA AGTCTGCATC CAGGACGAGC AGCTGGTGCT GGGAGCCTAC 
AGGGAGCCAC AGCAACACTG GGACCAGGAC TATGACCACT TCCTGCTTCC TCTCCTGGAC 
GACCAGGAGC CCTGCTACAT CCTGTACCGT CTCGACTCCC AGAATGCTTT GGGCTACGAG 
TGGATTTTCA TCTCCTGGTC TCCTGACCAG TCTCCAGTGA AGCAGAAGAT GTTGTACGCC 
GCCACTCGTG CCACAGTGAA GAAAGAGTTT GGCGGCGGCC ACGTGAAGTA CGAGTTATTT 
GGCACCACGG AGGAGGATGT CTGCTTGCAG GGCTACCAGC ATTACGTGTC GTCCTGCTCG 
GGTCCAGCTC CGCTCACGCT AGCTGAGCAG GAGCTGCAGA GGATCAAAAT CACAGAGGGC 
CAGGTAAAAC AGGTGAAGAC AGAAATCAGC GTGGAGAGCA AACACCAGAC ACTTCAGGGC 
CTCGCTTTTC CTCTGCAGGA GTCGGCCAAA AGAAGCCTAC ACCTGCTGGC CCAGAGGCGC 
ATTAACTATG TGCAGCTGAG GCTGGATGTG GAAAAAGAGA CGATAGAGCT GGTCCACGCC 
AACCCCACAG AAACTCGTGA TTTACCCCGC CGGGTTCCCA AAGACACTCC CAGATACCAC 
TTCTTCCTTT ACAAGCACTC CCATGAAGGA GATTACCTGG AATCTGTTGT CTTCATATAC 
TCCATGCCAG GATACAGCTG CAGCATTAAG GAGCGGATGC TGTATTCCAG CTGCAAAAGT 
CGACTACTGG AGGATGTGGA GAGGGATTAC CGCTTGGAAA TAGCCAAAAA GTTGGAGATT 
GACGATGGAG ACGACCTGAC GGAGGAATTT CTGTACGACG AGGTCCATCC TAAGCAGTAC 
GCTCACAAGC AGGCCTTTGC AAAGCCCCGA GGCCCAGCAG GAAAACGGGG ACATAAGCGT 
CTTATTAAGG GTGCAGGAGA GGCCATTCAG GACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twf2 ( XM_017304979.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_017160468.1 Poecilia reticulata twinfilin actin binding protein 2 (twf2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo195281
Clone ID Related Accession (Same CDS sequence)	XM_017304979.1
Accession Version	XM_017304979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	twinfilin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTTG CGTTGGTGGT GACGCCGGAA CTGCGGCAGT TCCTGGCTAG GGCAAGAGGC 
GGAGCGGCGC GCCTCATCAA AGTCTGCATC CAGGACGAGC AGCTGGTGCT GGGAGCCTAC 
AGGGAGCCAC AGCAACACTG GGACCAGGAC TATGACCACT TCCTGCTTCC TCTCCTGGAC 
GACCAGGAGC CCTGCTACAT CCTGTACCGT CTCGACTCCC AGAATGCTTT GGGCTACGAG 
TGGATTTTCA TCTCCTGGTC TCCTGACCAG TCTCCAGTGA AGCAGAAGAT GTTGTACGCC 
GCCACTCGTG CCACAGTGAA GAAAGAGTTT GGCGGCGGCC ACGTGAAGTA CGAGTTATTT 
GGCACCACGG AGGAGGATGT CTGCTTGCAG GGCTACCAGC ATTACGTGTC GTCCTGCTCG 
GGTCCAGCTC CGCTCACGCT AGCTGAGCAG GAGCTGCAGA GGATCAAAAT CACAGAGGTG 
AAGACAGAAA TCAGCGTGGA GAGCAAACAC CAGACACTTC AGGGCCTCGC TTTTCCTCTG 
CAGGAGTCGG CCAAAAGAAG CCTACACCTG CTGGCCCAGA GGCGCATTAA CTATGTGCAG 
CTGAGGCTGG ATGTGGAAAA AGAGACGATA GAGCTGGTCC ACGCCAACCC CACAGAAACT 
CGTGATTTAC CCCGCCGGGT TCCCAAAGAC ACTCCCAGAT ACCACTTCTT CCTTTACAAG 
CACTCCCATG AAGGAGATTA CCTGGAATCT GTTGTCTTCA TATACTCCAT GCCAGGATAC 
AGCTGCAGCA TTAAGGAGCG GATGCTGTAT TCCAGCTGCA AAAGTCGACT ACTGGAGGAT 
GTGGAGAGGG ATTACCGCTT GGAAATAGCC AAAAAGTTGG AGATTGACGA TGGAGACGAC 
CTGACGGAGG AATTTCTGTA CGACGAGGTC CATCCTAAGC AGTACGCTCA CAAGCAGGCC 
TTTGCAAAGC CCCGAGGCCC AGCAGGAAAA CGGGGACATA AGCGTCTTAT TAAGGGTGCA 
GGAGAGGCCA TTCAGGACAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017160468.1
CDS	333..1376
Translation	MFLALVVTPELRQFLARARGGAARLIKVCIQDEQLVLGAYREPQQHWDQDYDHFLLPLLDDQEPCYILYRLDSQNALGYEWIFISWSPDQSPVKQKMLYAATRATVKKEFGGGHVKYELFGTTEEDVCLQGYQHYVSSCSGPAPLTLAEQELQRIKITEVKTEISVESKHQTLQGLAFPLQESAKRSLHLLAQRRINYVQLRLDVEKETIELVHANPTETRDLPRRVPKDTPRYHFFLYKHSHEGDYLESVVFIYSMPGYSCSIKERMLYSSCKSRLLEDVERDYRLEIAKKLEIDDGDDLTEEFLYDEVHPKQYAHKQAFAKPRGPAGKRGHKRLIKGAGEAIQDS

Target ORF information:

RefSeq Version	XM_017304979.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata twinfilin actin binding protein 2 (twf2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017304979.1

ORF Insert Sequence:

ATGTTTCTTG CGTTGGTGGT GACGCCGGAA CTGCGGCAGT TCCTGGCTAG GGCAAGAGGC 
GGAGCGGCGC GCCTCATCAA AGTCTGCATC CAGGACGAGC AGCTGGTGCT GGGAGCCTAC 
AGGGAGCCAC AGCAACACTG GGACCAGGAC TATGACCACT TCCTGCTTCC TCTCCTGGAC 
GACCAGGAGC CCTGCTACAT CCTGTACCGT CTCGACTCCC AGAATGCTTT GGGCTACGAG 
TGGATTTTCA TCTCCTGGTC TCCTGACCAG TCTCCAGTGA AGCAGAAGAT GTTGTACGCC 
GCCACTCGTG CCACAGTGAA GAAAGAGTTT GGCGGCGGCC ACGTGAAGTA CGAGTTATTT 
GGCACCACGG AGGAGGATGT CTGCTTGCAG GGCTACCAGC ATTACGTGTC GTCCTGCTCG 
GGTCCAGCTC CGCTCACGCT AGCTGAGCAG GAGCTGCAGA GGATCAAAAT CACAGAGGTG 
AAGACAGAAA TCAGCGTGGA GAGCAAACAC CAGACACTTC AGGGCCTCGC TTTTCCTCTG 
CAGGAGTCGG CCAAAAGAAG CCTACACCTG CTGGCCCAGA GGCGCATTAA CTATGTGCAG 
CTGAGGCTGG ATGTGGAAAA AGAGACGATA GAGCTGGTCC ACGCCAACCC CACAGAAACT 
CGTGATTTAC CCCGCCGGGT TCCCAAAGAC ACTCCCAGAT ACCACTTCTT CCTTTACAAG 
CACTCCCATG AAGGAGATTA CCTGGAATCT GTTGTCTTCA TATACTCCAT GCCAGGATAC 
AGCTGCAGCA TTAAGGAGCG GATGCTGTAT TCCAGCTGCA AAAGTCGACT ACTGGAGGAT 
GTGGAGAGGG ATTACCGCTT GGAAATAGCC AAAAAGTTGG AGATTGACGA TGGAGACGAC 
CTGACGGAGG AATTTCTGTA CGACGAGGTC CATCCTAAGC AGTACGCTCA CAAGCAGGCC 
TTTGCAAAGC CCCGAGGCCC AGCAGGAAAA CGGGGACATA AGCGTCTTAT TAAGGGTGCA 
GGAGAGGCCA TTCAGGACAG CTAG