ero1a cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	ero1a
Entrez Gene ID	103462928
Full Name	endoplasmic reticulum oxidoreductase 1 alpha
Synonyms	ero1l
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008405996.2	XP_008404218.1	ERO1-like protein alpha

Pathways from BioSystems
Gene Ontology

The following ero1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ero1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo190875	XM_008405996.2 Latest version!	Poecilia reticulata endoplasmic reticulum oxidoreductase alpha (ero1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ero1a ( XM_008405996.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008404218.1 Poecilia reticulata endoplasmic reticulum oxidoreductase alpha (ero1a), mRNA.

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CloneID	OPo190875
Clone ID Related Accession (Same CDS sequence)	XM_008405996.2
Accession Version	XM_008405996.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	ERO1-like protein alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024333.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008405996.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGG TGGTCTTCCT CCTCCTCTTC CTCCTCCAGG TGTCCGGATC TGCACACGGC 
AGGTGTTTCT GTCAGGTCAC AGGTGAACTG GACGACTGTA CATGCGACGT CGAAACCATC 
GACAGTTTCA ACAACGAGCA GATTTTCCCC AGACTTCTAC CTCTCCTGGA GTCCCACTAC 
TTCAGGTTCT ACAAGGTGAA TCTGAACAAA CCGTGTCCTT TCTGGACGGC GGACAGCCAC 
TGCGGTCTGA GAGACTGCGC TGTGAAGCCT TGCTCACCTA ACGAAGTTCC AGAGGGAATC 
CGATCCGCTC CACACAACAA GTATTCTGCA GAGGCGAACA AGCAGCCGCT GACTGAATGT 
GAGAAGGAGA AACATCTGGG AGCCGTGGAT GTTTCTCTGA GTGAGAAGAC CAGAGAAGCT 
TTATTGGACT GGCGCAAACA CGATGACAAG GCCGAGCGTT TCTGCGTTAT TGACGACGAG 
GAGTCGCCGG ACTCCCAGTA CGTCGACCTG CTGCTGAACC CGGAGCGCTA CACGGGCTAC 
AAAGGTCCGG AGGCGTGGCA GATCTGGAAC AGCATCTACG AGGAGAACTG CTTCAAGCCG 
TTCACCGTCA AGCGACCGCT CATGCCCAGC TCCTTTCAGA GCAGCTCAGG AAGTGAAGCG 
CGGACCTTCT ACAGCTGGCT GGAAGGTCAG TGTGTGGAGA AGAGAGCTTT CTACCGTCTG 
ATATCTGGMC TCCACGCCAG CATCAACGTT CATCTGAGCG CACGGTACCT CCTGGACGAC 
ACTTGGTTTC AGAGGAAGTG GGGCCACAAT GTGTCTGAGT TCAGGCAGCG CTTCGACTCT 
GACCTGACGG CCGGCGAAGG CCCCAAGAGG CTCCGCAACC TCTACTTCCT GTACCTGATA 
GAGCTGAGAG CGCTGGCCAA AGTTCTGCCG TTCTTCAGTC AGCCGTCCTT CCAGTTGTAC 
ACGGGTCAGC CAGAGCAGGA CCAGAAATAC AAAGAGCTGC TTCTAGAGAT CCTGAATCTG 
GCCAAATCTT TTCCTCTGCA CTTTGATGAA ACTTCTCTGT TTGCTGGAGA TGAAAAAGAA 
GCAGCACAGC TAAAGGAGGA CATCAGGCTG GCGTTTCTCA ACATCTCCAG GATCATGGAC 
TGCGTCGGCT GCTTCAAATG TCGTGTCTGG GGAAAAGTAC AGACTCAGGG ATTAGGAACG 
GCTCTGAAGA TCCTGTTTTC AGAGCGACAG ATTGAAGCGC TGCCCCAGTC CACAGAGCAA 
CGACCCGGTT TCCAGCTCAG CCGGCAGGAA GTCGTCTCCC TCCTCAACGC CTTTGGAAGG 
ATTTCCACGA GCGTCAGAGA GCTGAAGACC TTCAGATCGC TGCTGGCAGA GGAGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008404218.1
CDS	347..1726
Translation	MESVVFLLLFLLQVSGSAHGRCFCQVTGELDDCTCDVETIDSFNNEQIFPRLLPLLESHYFRFYKVNLNKPCPFWTADSHCGLRDCAVKPCSPNEVPEGIRSAPHNKYSAEANKQPLTECEKEKHLGAVDVSLSEKTREALLDWRKHDDKAERFCVIDDEESPDSQYVDLLLNPERYTGYKGPEAWQIWNSIYEENCFKPFTVKRPLMPSSFQSSSGSEARTFYSWLEGQCVEKRAFYRLISnullLHASINVHLSARYLLDDTWFQRKWGHNVSEFRQRFDSDLTAGEGPKRLRNLYFLYLIELRALAKVLPFFSQPSFQLYTGQPEQDQKYKELLLEILNLAKSFPLHFDETSLFAGDEKEAAQLKEDIRLAFLNISRIMDCVGCFKCRVWGKVQTQGLGTALKILFSERQIEALPQSTEQRPGFQLSRQEVVSLLNAFGRISTSVRELKTFRSLLAEER

Target ORF information:

RefSeq Version	XM_008405996.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata endoplasmic reticulum oxidoreductase alpha (ero1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008405996.2

ORF Insert Sequence:

ATGGAGTCGG TGGTCTTCCT CCTCCTCTTC CTCCTCCAGG TGTCCGGATC TGCACACGGC 
AGGTGTTTCT GTCAGGTCAC AGGTGAACTG GACGACTGTA CATGCGACGT CGAAACCATC 
GACAGTTTCA ACAACGAGCA GATTTTCCCC AGACTTCTAC CTCTCCTGGA GTCCCACTAC 
TTCAGGTTCT ACAAGGTGAA TCTGAACAAA CCGTGTCCTT TCTGGACGGC GGACAGCCAC 
TGCGGTCTGA GAGACTGCGC TGTGAAGCCT TGCTCACCTA ACGAAGTTCC AGAGGGAATC 
CGATCCGCTC CACACAACAA GTATTCTGCA GAGGCGAACA AGCAGCCGCT GACTGAATGT 
GAGAAGGAGA AACATCTGGG AGCCGTGGAT GTTTCTCTGA GTGAGAAGAC CAGAGAAGCT 
TTATTGGACT GGCGCAAACA CGATGACAAG GCCGAGCGTT TCTGCGTTAT TGACGACGAG 
GAGTCGCCGG ACTCCCAGTA CGTCGACCTG CTGCTGAACC CGGAGCGCTA CACGGGCTAC 
AAAGGTCCGG AGGCGTGGCA GATCTGGAAC AGCATCTACG AGGAGAACTG CTTCAAGCCG 
TTCACCGTCA AGCGACCGCT CATGCCCAGC TCCTTTCAGA GCAGCTCAGG AAGTGAAGCG 
CGGACCTTCT ACAGCTGGCT GGAAGGTCAG TGTGTGGAGA AGAGAGCTTT CTACCGTCTG 
ATATCTGGMC TCCACGCCAG CATCAACGTT CATCTGAGCG CACGGTACCT CCTGGACGAC 
ACTTGGTTTC AGAGGAAGTG GGGCCACAAT GTGTCTGAGT TCAGGCAGCG CTTCGACTCT 
GACCTGACGG CCGGCGAAGG CCCCAAGAGG CTCCGCAACC TCTACTTCCT GTACCTGATA 
GAGCTGAGAG CGCTGGCCAA AGTTCTGCCG TTCTTCAGTC AGCCGTCCTT CCAGTTGTAC 
ACGGGTCAGC CAGAGCAGGA CCAGAAATAC AAAGAGCTGC TTCTAGAGAT CCTGAATCTG 
GCCAAATCTT TTCCTCTGCA CTTTGATGAA ACTTCTCTGT TTGCTGGAGA TGAAAAAGAA 
GCAGCACAGC TAAAGGAGGA CATCAGGCTG GCGTTTCTCA ACATCTCCAG GATCATGGAC 
TGCGTCGGCT GCTTCAAATG TCGTGTCTGG GGAAAAGTAC AGACTCAGGG ATTAGGAACG 
GCTCTGAAGA TCCTGTTTTC AGAGCGACAG ATTGAAGCGC TGCCCCAGTC CACAGAGCAA 
CGACCCGGTT TCCAGCTCAG CCGGCAGGAA GTCGTCTCCC TCCTCAACGC CTTTGGAAGG 
ATTTCCACGA GCGTCAGAGA GCTGAAGACC TTCAGATCGC TGCTGGCAGA GGAGCGATGA