fto cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	fto
Entrez Gene ID	103462660
Full Name	FTO, alpha-ketoglutarate dependent dioxygenase
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008405567.2	XP_008403789.1	alpha-ketoglutarate-dependent dioxygenase FTO

Pathways from BioSystems
Gene Ontology

The following fto gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fto cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo191771	XM_008405567.2 Latest version!	Poecilia reticulata fat mass and obesity associated (fto), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fto ( XM_008405567.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008403789.1 Poecilia reticulata fat mass and obesity associated (fto), mRNA.

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CloneID	OPo191771
Clone ID Related Accession (Same CDS sequence)	XM_008405567.2
Accession Version	XM_008405567.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	alpha-ketoglutarate-dependent dioxygenase FTO
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024333.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008405567.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCGA GGCAGTGTAA ACACAACTCC AGGAACATGA AAAGATCGGG TGACAGTGAG 
GGGGAGAAGA GAAGAAAGAG GCGTAGGCTA CTGCAGGAGT TGGGAGACCA GAAGATCCCT 
TTTCTGGGAC CTTCAGATCG AGGTTTTCAG CAACTGTGGG ATTCCAGCTA CTCTGGCTTG 
GTGTTGAGGA AATCGTGTTC GCTTCCTGCT GAGCTCCACA GCCGAGTCCA GGCGGCTCTG 
CTCACCCTCA GAAAGAAGGG CTGTCTCCTG AAGGATCTGG TCCGCGTCCG CGACCGAGAC 
GTGTTCACCG CCGTGTCGCG AGCGCTGCTG GGTGATCCCG GCCACACCTA CCGATATCTG 
GACACGCGGC TCTTTGCCAT CCCCTGGCAC AGCGAGGACG CCGAGGTTAA AGGACAAAAC 
TGCTGTGATC CCGATTTGAG GGACGCTTGC AAAGCATTGT GGGAGCTTAA CACATTCCTC 
TGTTCTGATG TGTCTCAACT GAAGGAGGGA GACCAGTTTT CACAATGTAT GAAGGCCGAA 
GGGGAAGCAA AACTAGTTGA AGAGGGTGAG ACAGAGTCCA AACACAGTGA CGACTCCAAG 
AATAGTGAAG GAGGGGATTC GGAGTCCAGA CAGAGCGAGG AGGGGGACAC CGAGTCCAAG 
CACAGCGAGG AGTGGGACAT TGAGTCCAAG CACAGCGAAG AAGGATGCTC TGGGTCGAAA 
CAAGAAGCGG GGTGGGAGAC TGAGGAGGGC GAGTCCTCCC AAGTCAAACC GAGTGAAGGT 
AGTTTTGCAG GAGTTTCAGA GCACAGCGAA GGAAAATGCC CCGGCTGGAC GACGGAGGCC 
TTCAGTGGGA CAAAGCCTGA TCCCGTGGGA CTAGAGGCTC AGGAGAAGCC TGTGGCTCAG 
CTAAAGCACA GCCTGTCAGA TTACCACATT CAGGATAAAG AGGAACTTGC AGGTGGGCTG 
GGCTGTTCGC AGCCCAGTCC TCCCCAGGTG TGCACCGGTC CAGTCAAGTT TAATGTCACC 
TTACTGAACT ACATGGACCC CACACAAATG AGCCACCTCA AGGAGGAGCC GTACTACGGC 
ATGGGGAAAA TGGCGGTAGG GTGGCACCAC GATGAGAACC TCATCACTCA TTCACCAGTG 
GCTGTTTACA ACTATAGTTG TCATGACGAC AAAGGTGAGT GCAGCGAAGG CAGCAGCGTG 
GAGAAGTCAT GTTGGAGGAT CGGCCTGAAG GTGGCCTGGG ACATCCACAC ACCTGGCCTG 
ATGCTGCCGC TGGAGTCCGG AGACTGCTAC TACATGAGAG ATGACCTGAA CAGCACCCAC 
CAGCACTGCG TCCTCGCTGG AGAAACGGCA CGCTTCAGCT CGACTCACAG AGTGGCCGAG 
TGTTCTAAGG GGACCCTGAC CTACATCCAG TCACGGTGCC AGGAGGCCCT GTCCAACCTG 
CACACTGACC CCGAGACGGG CTCCCACAGC CTGATGACCC TTCAGCCGAC AGCATTACAG 
CACTGTGAGG AGATTCACAA TGAGGTGGAG TTTGAATGGC TGCGGCAGTA TTGGTTTCAG 
GGCCAGCGTT ACGCACGCTT CTGCAGCTGG TGGACCCGAC CGATGGAGCA GCTGGAGAAG 
GACTGGAAGC TCATGGAGAC CATGACCATG CTTTTCCTGG CTACTGTGGA GGAGGAAGGC 
CAAGCCGAGG AGGGACGGAG GGAAATGGCC GAAACCCTGC TGTCTGCCCT GACAGACAGG 
CACCAGCAGA GGCAGACATG GAGAGACAGG TGCCACTCGA GTCTGGCGCA GACGTTACCC 
CCCGAGGAGG CCCCAGTGGA CCGACCCTTC TGGGGTGTGG ATGACCCCGG CATGCCTCTG 
CCCTTTGATC TGGCTGATAT CATCAGCAGA GTGGAGTCAC TGCTGTGGAG GATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008403789.1
CDS	143..2059
Translation	MKARQCKHNSRNMKRSGDSEGEKRRKRRRLLQELGDQKIPFLGPSDRGFQQLWDSSYSGLVLRKSCSLPAELHSRVQAALLTLRKKGCLLKDLVRVRDRDVFTAVSRALLGDPGHTYRYLDTRLFAIPWHSEDAEVKGQNCCDPDLRDACKALWELNTFLCSDVSQLKEGDQFSQCMKAEGEAKLVEEGETESKHSDDSKNSEGGDSESRQSEEGDTESKHSEEWDIESKHSEEGCSGSKQEAGWETEEGESSQVKPSEGSFAGVSEHSEGKCPGWTTEAFSGTKPDPVGLEAQEKPVAQLKHSLSDYHIQDKEELAGGLGCSQPSPPQVCTGPVKFNVTLLNYMDPTQMSHLKEEPYYGMGKMAVGWHHDENLITHSPVAVYNYSCHDDKGECSEGSSVEKSCWRIGLKVAWDIHTPGLMLPLESGDCYYMRDDLNSTHQHCVLAGETARFSSTHRVAECSKGTLTYIQSRCQEALSNLHTDPETGSHSLMTLQPTALQHCEEIHNEVEFEWLRQYWFQGQRYARFCSWWTRPMEQLEKDWKLMETMTMLFLATVEEEGQAEEGRREMAETLLSALTDRHQQRQTWRDRCHSSLAQTLPPEEAPVDRPFWGVDDPGMPLPFDLADIISRVESLLWRM

Target ORF information:

RefSeq Version	XM_008405567.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata fat mass and obesity associated (fto), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008405567.2

ORF Insert Sequence:

ATGAAAGCGA GGCAGTGTAA ACACAACTCC AGGAACATGA AAAGATCGGG TGACAGTGAG 
GGGGAGAAGA GAAGAAAGAG GCGTAGGCTA CTGCAGGAGT TGGGAGACCA GAAGATCCCT 
TTTCTGGGAC CTTCAGATCG AGGTTTTCAG CAACTGTGGG ATTCCAGCTA CTCTGGCTTG 
GTGTTGAGGA AATCGTGTTC GCTTCCTGCT GAGCTCCACA GCCGAGTCCA GGCGGCTCTG 
CTCACCCTCA GAAAGAAGGG CTGTCTCCTG AAGGATCTGG TCCGCGTCCG CGACCGAGAC 
GTGTTCACCG CCGTGTCGCG AGCGCTGCTG GGTGATCCCG GCCACACCTA CCGATATCTG 
GACACGCGGC TCTTTGCCAT CCCCTGGCAC AGCGAGGACG CCGAGGTTAA AGGACAAAAC 
TGCTGTGATC CCGATTTGAG GGACGCTTGC AAAGCATTGT GGGAGCTTAA CACATTCCTC 
TGTTCTGATG TGTCTCAACT GAAGGAGGGA GACCAGTTTT CACAATGTAT GAAGGCCGAA 
GGGGAAGCAA AACTAGTTGA AGAGGGTGAG ACAGAGTCCA AACACAGTGA CGACTCCAAG 
AATAGTGAAG GAGGGGATTC GGAGTCCAGA CAGAGCGAGG AGGGGGACAC CGAGTCCAAG 
CACAGCGAGG AGTGGGACAT TGAGTCCAAG CACAGCGAAG AAGGATGCTC TGGGTCGAAA 
CAAGAAGCGG GGTGGGAGAC TGAGGAGGGC GAGTCCTCCC AAGTCAAACC GAGTGAAGGT 
AGTTTTGCAG GAGTTTCAGA GCACAGCGAA GGAAAATGCC CCGGCTGGAC GACGGAGGCC 
TTCAGTGGGA CAAAGCCTGA TCCCGTGGGA CTAGAGGCTC AGGAGAAGCC TGTGGCTCAG 
CTAAAGCACA GCCTGTCAGA TTACCACATT CAGGATAAAG AGGAACTTGC AGGTGGGCTG 
GGCTGTTCGC AGCCCAGTCC TCCCCAGGTG TGCACCGGTC CAGTCAAGTT TAATGTCACC 
TTACTGAACT ACATGGACCC CACACAAATG AGCCACCTCA AGGAGGAGCC GTACTACGGC 
ATGGGGAAAA TGGCGGTAGG GTGGCACCAC GATGAGAACC TCATCACTCA TTCACCAGTG 
GCTGTTTACA ACTATAGTTG TCATGACGAC AAAGGTGAGT GCAGCGAAGG CAGCAGCGTG 
GAGAAGTCAT GTTGGAGGAT CGGCCTGAAG GTGGCCTGGG ACATCCACAC ACCTGGCCTG 
ATGCTGCCGC TGGAGTCCGG AGACTGCTAC TACATGAGAG ATGACCTGAA CAGCACCCAC 
CAGCACTGCG TCCTCGCTGG AGAAACGGCA CGCTTCAGCT CGACTCACAG AGTGGCCGAG 
TGTTCTAAGG GGACCCTGAC CTACATCCAG TCACGGTGCC AGGAGGCCCT GTCCAACCTG 
CACACTGACC CCGAGACGGG CTCCCACAGC CTGATGACCC TTCAGCCGAC AGCATTACAG 
CACTGTGAGG AGATTCACAA TGAGGTGGAG TTTGAATGGC TGCGGCAGTA TTGGTTTCAG 
GGCCAGCGTT ACGCACGCTT CTGCAGCTGG TGGACCCGAC CGATGGAGCA GCTGGAGAAG 
GACTGGAAGC TCATGGAGAC CATGACCATG CTTTTCCTGG CTACTGTGGA GGAGGAAGGC 
CAAGCCGAGG AGGGACGGAG GGAAATGGCC GAAACCCTGC TGTCTGCCCT GACAGACAGG 
CACCAGCAGA GGCAGACATG GAGAGACAGG TGCCACTCGA GTCTGGCGCA GACGTTACCC 
CCCGAGGAGG CCCCAGTGGA CCGACCCTTC TGGGGTGTGG ATGACCCCGG CATGCCTCTG 
CCCTTTGATC TGGCTGATAT CATCAGCAGA GTGGAGTCAC TGCTGTGGAG GATGTGA