nrde2 cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	nrde2
Entrez Gene ID	103458788
Full Name	NRDE-2, necessary for RNA interference, domain containing
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008399844.2	XP_008398066.1	protein NRDE2 homolog

Pathways from BioSystems
Gene Ontology

The following nrde2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nrde2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo188884	XM_008399844.2 Latest version!	Poecilia reticulata NRDE-2, necessary for RNA interference, domain containing (nrde2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nrde2 ( XM_008399844.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008398066.1 Poecilia reticulata NRDE-2, necessary for RNA interference, domain containing (nrde2), mRNA.

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CloneID	OPo188884
Clone ID Related Accession (Same CDS sequence)	XM_008399844.2
Accession Version	XM_008399844.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	protein NRDE2 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024352.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008399844.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCGACG CTATCATGGC TCTTTTTCCA GCGTTTGCTG AAGTATCAGA AAGCAAAGTT 
GAAGATTCGT CGAAAGAATT AGACTGGCTG AACAATAAGA GTTTTCAGGC TGGAAATGCT 
CTTTCCCTCC ACAGTCGTTT TTTAGAAAAG TCCACTGAAC AGCCAAACAA GAGGGTGGAA 
AGTGTTGGCA GGGATATTAC ATGCTCCGAA GGGGAAGGCA GTGGGAAGGA TGATGGGCCT 
CCAAAGAAGA AGAAAAAGAA GAGTGAAAAA AAGAGGAAAA AGAAGAAAAA GCACAAAAAA 
AAGAGTGGGA GGGCTTCGGG CAGTAGTGGA TCGGACTCTG AAACCATCTA TCCCAGTGAC 
CTCAAGAGGG AAGAGGAGGC GATTAGGCAA CAGCCTGCTT CATTAGGAAC TCGTTTTACA 
TGGTTGGATG ACCTCCAGTC ACATACAGAA AATCCGTTCT GTGTGGACCG TAAACCGGAC 
CCGGCCAACT GGACTTATAA GTCCCTGTAC AGAGGAGACG TAGCTAGGTA TCGGAGAAAA 
GGAAGTTCCT CTTTAGGTCT GGACTTTCGC AGGCAGGGGG TCAGCTGGGA GGATTCTGAG 
ACAAAGAAGA AGAAGAAAGT CGGGGTCAAT GGGAATGCAG CTGACAGGTA CTTTTCTGTA 
GCCACTCGAA AGCTGCTGCG GGCTGCGCCT CCTGTTCCCG TTATCCCTGC GACGCCAGAA 
AGTGGGGATG TACTCAACTC AACCTCCTTC CTCCCGCTGG GTGACGCTGA ATGGGACAAC 
ACAAGACATG AGTTAGATAG CAAAGCACAG ACGTCGGTAA ACCCCCTTGG AGTGTATGAT 
TTGTCCACCT CCCTGTGGAT GCAGGGGAAG GGACCACTGC TTCAGGACGA CGCGAAGCGG 
GTCGCAGAAA CAGAGGAAAA GCCCGAGTAT GTAGCTGGAA GGACCGAAGA GTTCAATAGG 
CGACTCAGAG AGCAGCCAGC AGATACACAG CTGTGGATAG AGTTCATTCG CTACCAGGAC 
GAGCTGAGTG CAGCTGCATT TGGAGGTGAC GAGGAGCAGC AGGGCAGTGA TTTGGCGGAG 
CGCCGCAAGT CGTCCTACAG GGCGATTCTG GAGAAGAAGC TGAGCATTGC AGACAGAGCC 
GTGGAAACCA ACCCCACCTG CGTCGCTCTG CAGCTCGAGA GGCTCCGGAT CTGCCAGGAT 
CTCTGGGAAC CCTCCACTTT GGCTAAAGAA TGGAAGAAAC TGGTGTTTCT CCATCCAAAC 
AGCGCCCCTT TGTGGAGGAA ATTTCTGCTT TTTACGCAGA GCTACTTCAG CAGCTTCTCA 
GTGACGAAAG TCAACTCTGC ATATGGAAAG TGTCTGAGCA CACTCAGCGC TGTGAGAGAC 
GGTAGCATGG TGTCCCACCC AGCTTTGCCT GGAATTGAGG AGGACATGCT GGATATCTTC 
ACCCAGCAGT GTCATTTCTT GCGGCAGTCT GGTCACTGTG AGAAGGCGAT TTCTCTGTTT 
CAGGCCATGA TCGATTTTAG CTACTTCAAA CCTGACAGTG TGAAAAAGCT CTCCACAAAG 
CAGCAGGTTG ATTTCTTTGA GACGTTCTGG GACAGTGGAG AGGCGAGAGT TGGGGAGGTA 
GGAGCCCGAG GATGGAAGGC CTGGATGACC CAACAAGAGC GAGGAGGATG GGTTCAGCCC 
CCTGCAGAGG AAGAAGAGGA GGAGGAGGAG GAAGAGGATG AAGAGGAGGT GAAAGACCGG 
AGCCAGCCCA GAGGGACCGT CTGGCTGGAT GTGGAGTCGT CCCGTGAAGC AGCTCACTGG 
TTGCCCTGGA GACCTGACAA AGCCAAAGGC CAATCAGAGG AGGACTGTGA GGACCCTGAC 
AGACAGGTGC TGTTTGATGA CATCGGCCCA TCCCTTATTT GTCTGACTTC ACCAGAACTC 
CAGCTGCGGT TTCTTCTCAG TTTCTTGGCC TTTCTGGGTT TCCCGGTGGA TTTTGTGCTC 
TCTGCTGCCC CCTATCAGCC GAGCATGCTC CTGGAGAACC TCTCACTGCT CACTCAAGGT 
GCAGCGCTCC AGCGTCCTCT GACTTCCTTT AACCCCGTTG ATACGGGGAT TAACCCTGTA 
GGTCACATGA CCACTCTCCA AGGAACCAGG AAGTGGTTGG GGCTTGGAAA GCAGGGTGAG 
AAGTTTCTCA CCAACGTGTT CAGCATGCTG CAGCCTTTCC TCCCTCCTCC CCATCGAGCC 
GTTCTGTCTC TCAGCTGGAT GCTGTACGAG AAGCTGAAGG TTTTGCACTG TCTGCGCAGC 
GGCAACAAAA AGCGGCTTCG CGCTCAGGGA AAGAGCAGCA AGCGGGTCGC CAAGAGACTC 
CTCAAAGAAC CCGACAATCG CTCGTCCCTG GTGCTGTGGA GGGAGTACGC ACACCTGGAG 
TGGGTGCTGG GCAACCTGGA CGAGGCTCGG AAGGTGTTCT CCACGGCGAC GGCGATGGGA 
GGAACCAGGG GCCTCAGCAG TCCTGCTCTG TGTGAGCTGT GCCTCCTGTG GTCCCAGCTG 
GAGGTGGAGA ACTCGGCAAA GGAGCCGGGA GGAGTCGCTG ACGTAACGAC GTCGCCAGCT 
GTTGCGGTGC TGACCAGACT GGCAGAAGGA ACGTCTTTGA CTTCATCCTC CCAGACCTTG 
ACCCCAGTTT CAGTCTTAAA GGCCAGGAGA TCGTATGATC AGGCTCTGAG CTCCAGCCTG 
TCATCTCTGG ACCAGAAGAT CCAGAATCAT CAGCAGGACG GAAACGCCCA AGATGAGCCG 
CAGGAAGAGA AGCTGAGGCT AAGAGGCCTG GTCGGCTGCT ACGCTCTGTT TCAGTACCTA 
ACAGTGAGCA TCCAAGCTGC TAATGCTGTC TACAGCCAGG CCAGGGAGAG GATGGAGGAG 
CTGCACCGCT CGTTAACACC CGAAAGGCAG CTCGACCACG ATGAGGCAAC CAGCCGGCCG 
AGTCGTACTG TCCACAGCCA GCATGTCAGC TGGTTGGCTT CAGAGTGTGA GGCTTTAGCA 
GTGCAACAGG CGGCTCTGCT CAGACACAAC AATAGTGTCG GTGTGTGTGC TTTGGCAACA 
CTGAGGCAGA TGCTTACCTG TTCCCTCTCA GCTTGGCCAT ACAGCGCCCC CCTGTGGAAT 
GTTTACATTC AGGTGGAAAA CCGTTACCAT AGCGCCGGCC GGGCACGCCG GTTTTTCCAC 
ACTGTAACCA GAGACAACAG CAGCAGCGTG GTACCCCGTC TGTTTGCGAT TGTTGCTGAA 
CAGCATAGGA AGCAGCTGGT GGATGCAGCT CAGAGGTCTT GTTGCTTGGA TGAGGCCCTG 
CCCATTCTAC CAGAAAATGG CCTCAGCAAC CGAATCCGGG GACTGTTTGA GGGTGCCACA 
TCAACAGAGA TCGGTGCTCA TTGTCCTCTA CTGTGGAGGA TGTACATCCA TTTCCTGGTG 
TCAGAGGGGA AGGTAGATAA AGCCAGAGGG ATATTTTACA AGGCATTGCA GAACATTCCC 
TGGGTGAAGG GTTTGTATAT GGATGCGGTG CAGTTGTTCC CTGACCACCT GCAGGAGTTT 
GTGGATCTGA TGACAGAGAA GGAACTCCGA CTAAGACTGC CTTTGGAGGA GTTGGATATA 
CTGCTGGAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008398066.1
CDS	41..3655
Translation	MCDAIMALFPAFAEVSESKVEDSSKELDWLNNKSFQAGNALSLHSRFLEKSTEQPNKRVESVGRDITCSEGEGSGKDDGPPKKKKKKSEKKRKKKKKHKKKSGRASGSSGSDSETIYPSDLKREEEAIRQQPASLGTRFTWLDDLQSHTENPFCVDRKPDPANWTYKSLYRGDVARYRRKGSSSLGLDFRRQGVSWEDSETKKKKKVGVNGNAADRYFSVATRKLLRAAPPVPVIPATPESGDVLNSTSFLPLGDAEWDNTRHELDSKAQTSVNPLGVYDLSTSLWMQGKGPLLQDDAKRVAETEEKPEYVAGRTEEFNRRLREQPADTQLWIEFIRYQDELSAAAFGGDEEQQGSDLAERRKSSYRAILEKKLSIADRAVETNPTCVALQLERLRICQDLWEPSTLAKEWKKLVFLHPNSAPLWRKFLLFTQSYFSSFSVTKVNSAYGKCLSTLSAVRDGSMVSHPALPGIEEDMLDIFTQQCHFLRQSGHCEKAISLFQAMIDFSYFKPDSVKKLSTKQQVDFFETFWDSGEARVGEVGARGWKAWMTQQERGGWVQPPAEEEEEEEEEEDEEEVKDRSQPRGTVWLDVESSREAAHWLPWRPDKAKGQSEEDCEDPDRQVLFDDIGPSLICLTSPELQLRFLLSFLAFLGFPVDFVLSAAPYQPSMLLENLSLLTQGAALQRPLTSFNPVDTGINPVGHMTTLQGTRKWLGLGKQGEKFLTNVFSMLQPFLPPPHRAVLSLSWMLYEKLKVLHCLRSGNKKRLRAQGKSSKRVAKRLLKEPDNRSSLVLWREYAHLEWVLGNLDEARKVFSTATAMGGTRGLSSPALCELCLLWSQLEVENSAKEPGGVADVTTSPAVAVLTRLAEGTSLTSSSQTLTPVSVLKARRSYDQALSSSLSSLDQKIQNHQQDGNAQDEPQEEKLRLRGLVGCYALFQYLTVSIQAANAVYSQARERMEELHRSLTPERQLDHDEATSRPSRTVHSQHVSWLASECEALAVQQAALLRHNNSVGVCALATLRQMLTCSLSAWPYSAPLWNVYIQVENRYHSAGRARRFFHTVTRDNSSSVVPRLFAIVAEQHRKQLVDAAQRSCCLDEALPILPENGLSNRIRGLFEGATSTEIGAHCPLLWRMYIHFLVSEGKVDKARGIFYKALQNIPWVKGLYMDAVQLFPDHLQEFVDLMTEKELRLRLPLEELDILLED

Target ORF information:

RefSeq Version	XM_008399844.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata NRDE-2, necessary for RNA interference, domain containing (nrde2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008399844.2

ORF Insert Sequence:

ATGTGCGACG CTATCATGGC TCTTTTTCCA GCGTTTGCTG AAGTATCAGA AAGCAAAGTT 
GAAGATTCGT CGAAAGAATT AGACTGGCTG AACAATAAGA GTTTTCAGGC TGGAAATGCT 
CTTTCCCTCC ACAGTCGTTT TTTAGAAAAG TCCACTGAAC AGCCAAACAA GAGGGTGGAA 
AGTGTTGGCA GGGATATTAC ATGCTCCGAA GGGGAAGGCA GTGGGAAGGA TGATGGGCCT 
CCAAAGAAGA AGAAAAAGAA GAGTGAAAAA AAGAGGAAAA AGAAGAAAAA GCACAAAAAA 
AAGAGTGGGA GGGCTTCGGG CAGTAGTGGA TCGGACTCTG AAACCATCTA TCCCAGTGAC 
CTCAAGAGGG AAGAGGAGGC GATTAGGCAA CAGCCTGCTT CATTAGGAAC TCGTTTTACA 
TGGTTGGATG ACCTCCAGTC ACATACAGAA AATCCGTTCT GTGTGGACCG TAAACCGGAC 
CCGGCCAACT GGACTTATAA GTCCCTGTAC AGAGGAGACG TAGCTAGGTA TCGGAGAAAA 
GGAAGTTCCT CTTTAGGTCT GGACTTTCGC AGGCAGGGGG TCAGCTGGGA GGATTCTGAG 
ACAAAGAAGA AGAAGAAAGT CGGGGTCAAT GGGAATGCAG CTGACAGGTA CTTTTCTGTA 
GCCACTCGAA AGCTGCTGCG GGCTGCGCCT CCTGTTCCCG TTATCCCTGC GACGCCAGAA 
AGTGGGGATG TACTCAACTC AACCTCCTTC CTCCCGCTGG GTGACGCTGA ATGGGACAAC 
ACAAGACATG AGTTAGATAG CAAAGCACAG ACGTCGGTAA ACCCCCTTGG AGTGTATGAT 
TTGTCCACCT CCCTGTGGAT GCAGGGGAAG GGACCACTGC TTCAGGACGA CGCGAAGCGG 
GTCGCAGAAA CAGAGGAAAA GCCCGAGTAT GTAGCTGGAA GGACCGAAGA GTTCAATAGG 
CGACTCAGAG AGCAGCCAGC AGATACACAG CTGTGGATAG AGTTCATTCG CTACCAGGAC 
GAGCTGAGTG CAGCTGCATT TGGAGGTGAC GAGGAGCAGC AGGGCAGTGA TTTGGCGGAG 
CGCCGCAAGT CGTCCTACAG GGCGATTCTG GAGAAGAAGC TGAGCATTGC AGACAGAGCC 
GTGGAAACCA ACCCCACCTG CGTCGCTCTG CAGCTCGAGA GGCTCCGGAT CTGCCAGGAT 
CTCTGGGAAC CCTCCACTTT GGCTAAAGAA TGGAAGAAAC TGGTGTTTCT CCATCCAAAC 
AGCGCCCCTT TGTGGAGGAA ATTTCTGCTT TTTACGCAGA GCTACTTCAG CAGCTTCTCA 
GTGACGAAAG TCAACTCTGC ATATGGAAAG TGTCTGAGCA CACTCAGCGC TGTGAGAGAC 
GGTAGCATGG TGTCCCACCC AGCTTTGCCT GGAATTGAGG AGGACATGCT GGATATCTTC 
ACCCAGCAGT GTCATTTCTT GCGGCAGTCT GGTCACTGTG AGAAGGCGAT TTCTCTGTTT 
CAGGCCATGA TCGATTTTAG CTACTTCAAA CCTGACAGTG TGAAAAAGCT CTCCACAAAG 
CAGCAGGTTG ATTTCTTTGA GACGTTCTGG GACAGTGGAG AGGCGAGAGT TGGGGAGGTA 
GGAGCCCGAG GATGGAAGGC CTGGATGACC CAACAAGAGC GAGGAGGATG GGTTCAGCCC 
CCTGCAGAGG AAGAAGAGGA GGAGGAGGAG GAAGAGGATG AAGAGGAGGT GAAAGACCGG 
AGCCAGCCCA GAGGGACCGT CTGGCTGGAT GTGGAGTCGT CCCGTGAAGC AGCTCACTGG 
TTGCCCTGGA GACCTGACAA AGCCAAAGGC CAATCAGAGG AGGACTGTGA GGACCCTGAC 
AGACAGGTGC TGTTTGATGA CATCGGCCCA TCCCTTATTT GTCTGACTTC ACCAGAACTC 
CAGCTGCGGT TTCTTCTCAG TTTCTTGGCC TTTCTGGGTT TCCCGGTGGA TTTTGTGCTC 
TCTGCTGCCC CCTATCAGCC GAGCATGCTC CTGGAGAACC TCTCACTGCT CACTCAAGGT 
GCAGCGCTCC AGCGTCCTCT GACTTCCTTT AACCCCGTTG ATACGGGGAT TAACCCTGTA 
GGTCACATGA CCACTCTCCA AGGAACCAGG AAGTGGTTGG GGCTTGGAAA GCAGGGTGAG 
AAGTTTCTCA CCAACGTGTT CAGCATGCTG CAGCCTTTCC TCCCTCCTCC CCATCGAGCC 
GTTCTGTCTC TCAGCTGGAT GCTGTACGAG AAGCTGAAGG TTTTGCACTG TCTGCGCAGC 
GGCAACAAAA AGCGGCTTCG CGCTCAGGGA AAGAGCAGCA AGCGGGTCGC CAAGAGACTC 
CTCAAAGAAC CCGACAATCG CTCGTCCCTG GTGCTGTGGA GGGAGTACGC ACACCTGGAG 
TGGGTGCTGG GCAACCTGGA CGAGGCTCGG AAGGTGTTCT CCACGGCGAC GGCGATGGGA 
GGAACCAGGG GCCTCAGCAG TCCTGCTCTG TGTGAGCTGT GCCTCCTGTG GTCCCAGCTG 
GAGGTGGAGA ACTCGGCAAA GGAGCCGGGA GGAGTCGCTG ACGTAACGAC GTCGCCAGCT 
GTTGCGGTGC TGACCAGACT GGCAGAAGGA ACGTCTTTGA CTTCATCCTC CCAGACCTTG 
ACCCCAGTTT CAGTCTTAAA GGCCAGGAGA TCGTATGATC AGGCTCTGAG CTCCAGCCTG 
TCATCTCTGG ACCAGAAGAT CCAGAATCAT CAGCAGGACG GAAACGCCCA AGATGAGCCG 
CAGGAAGAGA AGCTGAGGCT AAGAGGCCTG GTCGGCTGCT ACGCTCTGTT TCAGTACCTA 
ACAGTGAGCA TCCAAGCTGC TAATGCTGTC TACAGCCAGG CCAGGGAGAG GATGGAGGAG 
CTGCACCGCT CGTTAACACC CGAAAGGCAG CTCGACCACG ATGAGGCAAC CAGCCGGCCG 
AGTCGTACTG TCCACAGCCA GCATGTCAGC TGGTTGGCTT CAGAGTGTGA GGCTTTAGCA 
GTGCAACAGG CGGCTCTGCT CAGACACAAC AATAGTGTCG GTGTGTGTGC TTTGGCAACA 
CTGAGGCAGA TGCTTACCTG TTCCCTCTCA GCTTGGCCAT ACAGCGCCCC CCTGTGGAAT 
GTTTACATTC AGGTGGAAAA CCGTTACCAT AGCGCCGGCC GGGCACGCCG GTTTTTCCAC 
ACTGTAACCA GAGACAACAG CAGCAGCGTG GTACCCCGTC TGTTTGCGAT TGTTGCTGAA 
CAGCATAGGA AGCAGCTGGT GGATGCAGCT CAGAGGTCTT GTTGCTTGGA TGAGGCCCTG 
CCCATTCTAC CAGAAAATGG CCTCAGCAAC CGAATCCGGG GACTGTTTGA GGGTGCCACA 
TCAACAGAGA TCGGTGCTCA TTGTCCTCTA CTGTGGAGGA TGTACATCCA TTTCCTGGTG 
TCAGAGGGGA AGGTAGATAA AGCCAGAGGG ATATTTTACA AGGCATTGCA GAACATTCCC 
TGGGTGAAGG GTTTGTATAT GGATGCGGTG CAGTTGTTCC CTGACCACCT GCAGGAGTTT 
GTGGATCTGA TGACAGAGAA GGAACTCCGA CTAAGACTGC CTTTGGAGGA GTTGGATATA 
CTGCTGGAAG ACTGA