LOC106917887 cDNA ORF clone, Poecilia mexicana

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106917887
Entrez Gene ID	106917887
Full Name	threonine--tRNA ligase, cytoplasmic-like
Gene Type	protein-coding
Organism	Poecilia mexicana

mRNA and Protein(s)

mRNA	Protein	Name
XM_014987180.1	XP_014842666.1	regulation of nuclear pre-mRNA domain-containing protein 2-like isoform X1
XM_014987181.1	XP_014842667.1	threonine--tRNA ligase, cytoplasmic-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC106917887 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106917887 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo44008	XM_014987181.1 Latest version!	Poecilia mexicana threonine--tRNA ligase, cytoplasmic-like (LOC106917887), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPo44006	XM_014987180.1 Latest version!	Poecilia mexicana threonine--tRNA ligase, cytoplasmic-like (LOC106917887), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106917887 ( XM_014987181.1 ) cDNA ORF clone, Poecilia mexicana -> XP_014842667.1 Poecilia mexicana threonine--tRNA ligase, cytoplasmic-like (LOC106917887), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPo44008
Clone ID Related Accession (Same CDS sequence)	XM_014987181.1
Accession Version	XM_014987181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Poecilia mexicana
Product	threonine--tRNA ligase, cytoplasmic-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094783.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia mexicana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGA CGCAGCTGCT TAAGCTGCTC GTGAGTAGGT CGACACTCCA CGCACGAAGG 
AGATACAGCA AGCTCCAGCT GTCTCCAGCC CTGTCTGAGC GCCTGCGCGT CTTTGAGTCT 
CTCAGGGAAC AAAGAGACGA GAACAGGAGA CGTGAAGTTA GAGAAAGCCC TCTCTCCGTC 
CGCCTAACTA ATGGACAGAC GGTGAAGGGA ACAGCTGGAG TAACCACACC GCGTTACGTT 
GCACAGAGTC TGAGGGTGAA AGGAGCGCTG GTGTGCAAGG TGAATGGGGA GCTGTGGGAG 
CTGGGGCGAC CTCTAGAGGC AGACTGTGAA CTGCAGCTTC TGGGGTTTGA CTCGGCTGAA 
GGCAGACAGG CAGCATGGAG GACAGGAGCG TGTGTCCTTG GCGGTGTGTT GGAGGGGGCG 
TTTGCTGCAG AGGTCTGCAG GAAAGGAGCG TCGGAGCTCG GACTTTTTTG CGACCACGTC 
CCAAGCAGCA GTTCCTTGTC GCTGAGTGAT GTGGAGGCAA AATGTAAGGA GGCTGCAGCC 
CTCAAACTTC CTTTAGTCGG ACTGGACCTG AACACAGCAG AGCTTCATGA ACTGTTTCAG 
AGCAGTAAAC TGCGGATGCA GCTTCTAGAG GAGCAGATTA ACGGCCCCAC AGTCACAGTC 
TACAGATGTG GGGACAGGTT AGCGGTGTGT GACGGGCCTC TCCTCCCACA CACAGGTTTC 
CTCAAAGTCT TTAAGATGCT GCAGCTGTCC TCCGTCACGC TGTCCAACCA GACGGAGCCC 
AGCGGCTTAA CGCGTCTGCT AGGTGTGGCG TTTCCAGGAG AGAAGGACAG GGAGGAGTGG 
GAGAGGGAAC AGGAAGAGGC GCGGAGGAGA GACCACCGGC GGATAGGAAC AGACCAGGAG 
TTGTTCTTCT TCAACGAAGT GAGCCCAGGC AGCTGCTTCT TCCTGCCTAA AGGAGCCCAC 
ATCTACAACA CACTCACTGA CTTTATTAAG AGCGAGTACC GAAGGCGAGG CTTCACCGAG 
GTCGTGACCC CGACTCTTTA CAGCACCGCG TTATGGGAGC GCTCGGGCCA CTGGGAGCAC 
TACAGCAAGA ACATGTTCAC CGTGACCTCA GAGGGCGCTC AGACCTTCGC TCTGAAGCCC 
ATGAACTGCC CTGCACACTG TCTCATGTTT GAGCATCGGG TTCGCTCGTG GCGGGAGCTT 
CCTCTGCGCT GGGCCGACTT CGGGGCTCTG CATCGTAATG AGCTGTCTGG AGCTCTGGGA 
GGGCTGACTC GGGTCCGGAG GTTCTGCCAG GACGACGCAC ACATTTTTTG CACACCGGAG 
CAGCTGGAAC AGGAGATTGT GGCTTGTTTG GACTTTGTTC GGAGCGTCTA TCAAGTGTTT 
GGGTTTTCCT TCCACTGCTT GCTGTCCACA CGTCCCACTC CGTGCCTGGG GGAGCCTGAA 
CAGTGGGACC ACGCCGAACA GCAGCTGGAG AAGAGTCTGC AGCAGTTCGC CGAACGTTGG 
GAGCTGAACC CTGGAGATGG AGCTTTCTAC GGACCAAAGA TCGACATCCA GATTAAAGAT 
GCGATTGGCC GACAACACCA GTGTGCCACA ATCCAGCTCG ACTTCCAGCT GCCCATCAGA 
TTCAACCTTC AATACGTCGG ACGAGACGGA CAGCTGCACA GACCAGTGAT GATCCACAGG 
GCTGTACTGG GGTCACTGGA GAGAATGATC GCTATACTGG CTGAAAACTT TGGAGGCAAA 
TGGCCGCTGT GGTTGTCTCC GTCTCAGATC ATGGTGATTC CTGTAGGGGG CAACAGTGAG 
TCATACTCTA GACAGGTGGT CGCGACGTTC CACAACGCTG GCTTCATGGT GGATTTCAAC 
GACGATCCAG GAGCCACCTT AAATAAGAAG ATTCGCTCCG CTCAGCTGGC CCAGTACAAC 
TACATATTTG TGGTGGGTGA TAAGGAGCGT GAGAGTGGAG CAGTGAACGT GAGGAGCAGA 
GGGGGCAAAC AGTTGGGCAG CAGACAAACG GAGGAGGTGC TGAGGTCCCT CATACGTCTA 
CGAGACACCA GGAGCAACCA AGAGGAGCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014842667.1
CDS	348..2420
Translation	MAVTQLLKLLVSRSTLHARRRYSKLQLSPALSERLRVFESLREQRDENRRREVRESPLSVRLTNGQTVKGTAGVTTPRYVAQSLRVKGALVCKVNGELWELGRPLEADCELQLLGFDSAEGRQAAWRTGACVLGGVLEGAFAAEVCRKGASELGLFCDHVPSSSSLSLSDVEAKCKEAAALKLPLVGLDLNTAELHELFQSSKLRMQLLEEQINGPTVTVYRCGDRLAVCDGPLLPHTGFLKVFKMLQLSSVTLSNQTEPSGLTRLLGVAFPGEKDREEWEREQEEARRRDHRRIGTDQELFFFNEVSPGSCFFLPKGAHIYNTLTDFIKSEYRRRGFTEVVTPTLYSTALWERSGHWEHYSKNMFTVTSEGAQTFALKPMNCPAHCLMFEHRVRSWRELPLRWADFGALHRNELSGALGGLTRVRRFCQDDAHIFCTPEQLEQEIVACLDFVRSVYQVFGFSFHCLLSTRPTPCLGEPEQWDHAEQQLEKSLQQFAERWELNPGDGAFYGPKIDIQIKDAIGRQHQCATIQLDFQLPIRFNLQYVGRDGQLHRPVMIHRAVLGSLERMIAILAENFGGKWPLWLSPSQIMVIPVGGNSESYSRQVVATFHNAGFMVDFNDDPGATLNKKIRSAQLAQYNYIFVVGDKERESGAVNVRSRGGKQLGSRQTEEVLRSLIRLRDTRSNQEEL

Target ORF information:

RefSeq Version	XM_014987181.1
Organism	Poecilia mexicana
Definition	Poecilia mexicana threonine--tRNA ligase, cytoplasmic-like (LOC106917887), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014987181.1

ORF Insert Sequence:

ATGGCGGTGA CGCAGCTGCT TAAGCTGCTC GTGAGTAGGT CGACACTCCA CGCACGAAGG 
AGATACAGCA AGCTCCAGCT GTCTCCAGCC CTGTCTGAGC GCCTGCGCGT CTTTGAGTCT 
CTCAGGGAAC AAAGAGACGA GAACAGGAGA CGTGAAGTTA GAGAAAGCCC TCTCTCCGTC 
CGCCTAACTA ATGGACAGAC GGTGAAGGGA ACAGCTGGAG TAACCACACC GCGTTACGTT 
GCACAGAGTC TGAGGGTGAA AGGAGCGCTG GTGTGCAAGG TGAATGGGGA GCTGTGGGAG 
CTGGGGCGAC CTCTAGAGGC AGACTGTGAA CTGCAGCTTC TGGGGTTTGA CTCGGCTGAA 
GGCAGACAGG CAGCATGGAG GACAGGAGCG TGTGTCCTTG GCGGTGTGTT GGAGGGGGCG 
TTTGCTGCAG AGGTCTGCAG GAAAGGAGCG TCGGAGCTCG GACTTTTTTG CGACCACGTC 
CCAAGCAGCA GTTCCTTGTC GCTGAGTGAT GTGGAGGCAA AATGTAAGGA GGCTGCAGCC 
CTCAAACTTC CTTTAGTCGG ACTGGACCTG AACACAGCAG AGCTTCATGA ACTGTTTCAG 
AGCAGTAAAC TGCGGATGCA GCTTCTAGAG GAGCAGATTA ACGGCCCCAC AGTCACAGTC 
TACAGATGTG GGGACAGGTT AGCGGTGTGT GACGGGCCTC TCCTCCCACA CACAGGTTTC 
CTCAAAGTCT TTAAGATGCT GCAGCTGTCC TCCGTCACGC TGTCCAACCA GACGGAGCCC 
AGCGGCTTAA CGCGTCTGCT AGGTGTGGCG TTTCCAGGAG AGAAGGACAG GGAGGAGTGG 
GAGAGGGAAC AGGAAGAGGC GCGGAGGAGA GACCACCGGC GGATAGGAAC AGACCAGGAG 
TTGTTCTTCT TCAACGAAGT GAGCCCAGGC AGCTGCTTCT TCCTGCCTAA AGGAGCCCAC 
ATCTACAACA CACTCACTGA CTTTATTAAG AGCGAGTACC GAAGGCGAGG CTTCACCGAG 
GTCGTGACCC CGACTCTTTA CAGCACCGCG TTATGGGAGC GCTCGGGCCA CTGGGAGCAC 
TACAGCAAGA ACATGTTCAC CGTGACCTCA GAGGGCGCTC AGACCTTCGC TCTGAAGCCC 
ATGAACTGCC CTGCACACTG TCTCATGTTT GAGCATCGGG TTCGCTCGTG GCGGGAGCTT 
CCTCTGCGCT GGGCCGACTT CGGGGCTCTG CATCGTAATG AGCTGTCTGG AGCTCTGGGA 
GGGCTGACTC GGGTCCGGAG GTTCTGCCAG GACGACGCAC ACATTTTTTG CACACCGGAG 
CAGCTGGAAC AGGAGATTGT GGCTTGTTTG GACTTTGTTC GGAGCGTCTA TCAAGTGTTT 
GGGTTTTCCT TCCACTGCTT GCTGTCCACA CGTCCCACTC CGTGCCTGGG GGAGCCTGAA 
CAGTGGGACC ACGCCGAACA GCAGCTGGAG AAGAGTCTGC AGCAGTTCGC CGAACGTTGG 
GAGCTGAACC CTGGAGATGG AGCTTTCTAC GGACCAAAGA TCGACATCCA GATTAAAGAT 
GCGATTGGCC GACAACACCA GTGTGCCACA ATCCAGCTCG ACTTCCAGCT GCCCATCAGA 
TTCAACCTTC AATACGTCGG ACGAGACGGA CAGCTGCACA GACCAGTGAT GATCCACAGG 
GCTGTACTGG GGTCACTGGA GAGAATGATC GCTATACTGG CTGAAAACTT TGGAGGCAAA 
TGGCCGCTGT GGTTGTCTCC GTCTCAGATC ATGGTGATTC CTGTAGGGGG CAACAGTGAG 
TCATACTCTA GACAGGTGGT CGCGACGTTC CACAACGCTG GCTTCATGGT GGATTTCAAC 
GACGATCCAG GAGCCACCTT AAATAAGAAG ATTCGCTCCG CTCAGCTGGC CCAGTACAAC 
TACATATTTG TGGTGGGTGA TAAGGAGCGT GAGAGTGGAG CAGTGAACGT GAGGAGCAGA 
GGGGGCAAAC AGTTGGGCAG CAGACAAACG GAGGAGGTGC TGAGGTCCCT CATACGTCTA 
CGAGACACCA GGAGCAACCA AGAGGAGCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106917887 ( XM_014987180.1 ) cDNA ORF clone, Poecilia mexicana -> XP_014842666.1 Poecilia mexicana threonine--tRNA ligase, cytoplasmic-like (LOC106917887), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo44006
Clone ID Related Accession (Same CDS sequence)	XM_014987180.1
Accession Version	XM_014987180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Poecilia mexicana
Product	regulation of nuclear pre-mRNA domain-containing protein 2-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094783.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia mexicana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG GTTCAGGCGC TGCAAGCGGG CAGGGAGCCC GCAGCTCCAC TGGTTCTCTG 
GAAGCCTCAC TGGACCGCCG CTTTCAAGGA GTTTCCAATA CTATGGAGTC GATACAGAGC 
CTCTCGACGT GGTGCATTGA AAACAAGAAA CACCACGGCC TCATTGTTCG CTACTGGATG 
AAGTGGCTCA AGAAATCCGA CCACAATCAT CGCTTGAATC TTTTCTACCT TGCCAATGAT 
GTGATCCAAA ACTGCAAAAG AAAGAACGCC ATTGTGTTTC GTTCGGCGTT CTCCGATGTA 
CTTCCCAATG CTGCCCTCCT CATCAAAGAT GGAAAAGTCC GCAAATCGGT CGAAAGGATC 
TTTTCCATTT GGGAGGAGAG GAATGTGTAT CCCGAGGAAG TGATTGCACA GTTCAAAGCC 
AGCCTGCACA AAAAGGAAAA GGAGCGAGAA AAGCAGAAGG AGAAAGAGAA AGAAAAGCAG 
AAAGAAAAGG AGACTCCATC AAATAATGCT CCCACCAGCA CCAAAGCCGT CAGCACTAAA 
ACTCCACCTA ATAAAGCTGC GCTGAAGTCC AAGATTGTTG CAGAGTTTAC ACCCCACTCC 
CTTATCGAGC AGCTGTCGAG ACACAAGCAA GCTGCTGCTG AAGAGCAGGT CAGAGAGAAG 
CAGCTTGAGA CGCTCAGAGT GGATGTCCTC AGCACCGAGG CCTTCAAAAG GCTGAAAGAC 
AAAGCAGGAG GAAACAATTT TGCTAAGGAC TTTGAAGATG GCAGTCTGAA GCTGCAGGAG 
TTTGTCAGCT TCCTGGAGAA AGAGCTGAAA ACAGGACCTC CCCTGCTGGA AGCATTGGGA 
AATGCAGACA TCTTCTATGA GATGCAATAC AAGGAGGTTA AGATTGTGGC AAATGCGTAC 
AATGCCTTTG CCAACCGAGT AAACGGCCTC AAAAAGAAAC TGGATTCTCT CAAGGCGTCT 
CTCTCCGGCC CTGAGGACTC GCCCATCCCC TCTCCCTCTG AAGACGCTCC TTCACCCACT 
GGCTCCGACT CACCCTTTCT GGAAATGGAG ACGGCCAGGG CCAAGGTGGA TCCGGAGCTG 
GACGGCAAGG CCATGGACGA AGGAGAGCTC CAAAACGACA ACAGAGACAT GGAAGACATG 
GACATGTCTG ATGAGGAGGC ATGCGCTGGT GCTGCGCAGG ACGGTAAGTT AGACCAGCCG 
GCTGTTTCCA AGACGAAGTC GGACGAGGCG TCAAAACCTC AAAGCACGCC AACGAAACCA 
CCCAAGCCCA GCCCATCTGC TGTTACCACA GCAACCCCAG TGACTCCGAC CTCTGCTAAA 
GCTACGACAA CCCCGACCAC GCCCCTGGGA GTGAACCTGG CCAAAGTGGA CCTGGGGAAG 
ATCAGCTCCA TCCTCAACTC GCTCACGTCT GCCATGAAGA ACACAGCACC TACTGCGTCT 
CCTCGGCCGT CTCCTGGGAC CCCCACCACC CCCACCGCTC AGACAGCGGC CTCCAAAGGG 
ACGCCGCCAA GTCCTGCTTT GGCCAGCATC CTGTCACGTG TTGACATCAC CCCTGAAGGG 
ATACTAAACG CTCTGTCTAA AACCAACACA CCAGGTTTGT CATCCCTCCT TCAAAGTGTG 
ACTAATACCT CCACCGCTCC CTCCAACCGA ACCTCCACAG ACTCATCCAG TGTCAAAACC 
CCGGTAACCC CAACCACCCC GAAGACGAAG CCCCCTCTGG GGAACAGTCT CAAACAAGAC 
GAACCTGGAA GAGCCAGAGA CTGGGAGAAG GAGCGCCAGC TCTCCCCTCC ACCTCCTCCT 
CCACCCCCTC CTCGTACCTC AGCTCCTTCT TCCTCTCCCC CCAGCCTAGA ATCGAAGATC 
AACAGTTTCC TGCAGGGTAA TCCAGGCTTC AGCCTGGCGC TGGGCGACGC AAGTCCCGAT 
GGCGTCGACG GCACCCCGGT GAGGGACGAG GCAGCGGGCA CGCCGACGCA GGACGAGATC 
ATGGACACTC CTGGAGGCGT GCCAGAATCT CTGGGCTCCT CTGGAAGCCA TAACCTCTCA 
CCTACAGCCT ACCGCAATGA TCCCTGGGAT ACTGCAGTCA CTCCAACCGG AAGCAGCAAC 
AACGGAGACT TTCTTGGCTC CTCCTCCTCA AGATTCGGTG CAGGAAAAAG GAGCGGCACG 
AAGTTAAACG ACGAAGACCT GATGAGGAAG CAGGTGTCGT CTTCTCCTGG CGGTGACTTG 
AAGGCTCGGA ACAGCAGAGC CTTGGCAGAG CGGAGAATCT CTGCTGGCTC TCGGAAGGCG 
AGCACCGGTT CCGACGACGC GAGTCTGAGT GGAAAAAAGA ACAATGGGAA AGAGATGCCA 
GCAGACGCAA AGGAAGGTCA GTACCATCGC ATCGAGACAC TGGTGTCGCC CCACACCGAA 
GGAGCACCGA TCCAAACTCT GGGGTATTCA AACCGACCTT CAGCCGGAGA GCGCATCAAG 
ACAGTTGAGA GCATCCGCGT GATCGGCCGA GGTTCTCGAC GAGGGGGAGG AGCTGGGAGT 
CGGCCAGGGG CTTCGATGTG GTACGAAGAG GAAGAATACT TGGAGGGTCA GCCTCCATCA 
CCCCAAGTTC TACCCCCTCC TCTCAGCATG GGCCAGGGAG GCGCTGAAGA CATGAACCCC 
TCCATGCTTC CTCTTCCTCT TCCTCCTCCT CCACCACACC ACATCCCACA CCTTCACCCT 
CCTCCGTCTC TTCCTCTCCC TCCTCCTCAG CCTCCGTTCC CAATGCCGTA CCACCCTGAA 
AACATGCAGA CTCCTCCTCC ATCGATGCTC CAACCTCATC TCCATCCCTC TGCCGCCTTT 
TTCGCCACTG TTCCCCCAAT CCCTCGGCTG CCTCCACCTC CTTCCATGCA CCGCCTCTCA 
CCTCCGCCGT CTCACCCTTC TTTGCCCCAC TCCGTCATGG TGGGGGGGAT GCTGGTCCCT 
GTGGACCACA GCTTGCCTCT TCCTCCATCA CTAAGACCCA AAGGTACAGA GCACAGCAGA 
ATGGGGCCCA GAGGAAGGAA GGTGACCCCT CCACCACTCA TGTCATCGTT GCTAGGCGAC 
CCCCCTAAAC TACCCCGTCC CGGCACAGTT AAAGAGCCTT TAATCCCTCG CCATACCCCC 
CCTCTTCACC GCCCAGGTGC GCCTGGCGTT CCTCCGCCTT TACTCGGCAG AGTGAAGGAG 
CCTTTGGTTT TACCTCACCC CTCCCCCACA TCCTCCACGC CCTCTCCCTC CACGCCTAAT 
TCCCCCGCAG GAGACTCCTC TCTCTCCAAA CCCCCACCCA GCCCTCCAGC TGCGCCCCAC 
AACCCAGGCT CCAACCCCGT CCCCCTCCTC AACTTGCCCA CATCCAGACC CCCGATCCTC 
GCCACTCCCA TGCAGCAGAG ACCCCCGATG CGAGGCCGAA GCCTGTCTCA AGACCACCCC 
AGGGGCGGGT TTCATGGAGG GAAGCGAGCC GGACCCCCAT TCGGCGGCGG TCCCTTCCAT 
GCGCAGAAGA GGCCTTACCT TCCCCCACGC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014842666.1
CDS	53..3568
Translation	MAAGSGAASGQGARSSTGSLEASLDRRFQGVSNTMESIQSLSTWCIENKKHHGLIVRYWMKWLKKSDHNHRLNLFYLANDVIQNCKRKNAIVFRSAFSDVLPNAALLIKDGKVRKSVERIFSIWEERNVYPEEVIAQFKASLHKKEKEREKQKEKEKEKQKEKETPSNNAPTSTKAVSTKTPPNKAALKSKIVAEFTPHSLIEQLSRHKQAAAEEQVREKQLETLRVDVLSTEAFKRLKDKAGGNNFAKDFEDGSLKLQEFVSFLEKELKTGPPLLEALGNADIFYEMQYKEVKIVANAYNAFANRVNGLKKKLDSLKASLSGPEDSPIPSPSEDAPSPTGSDSPFLEMETARAKVDPELDGKAMDEGELQNDNRDMEDMDMSDEEACAGAAQDGKLDQPAVSKTKSDEASKPQSTPTKPPKPSPSAVTTATPVTPTSAKATTTPTTPLGVNLAKVDLGKISSILNSLTSAMKNTAPTASPRPSPGTPTTPTAQTAASKGTPPSPALASILSRVDITPEGILNALSKTNTPGLSSLLQSVTNTSTAPSNRTSTDSSSVKTPVTPTTPKTKPPLGNSLKQDEPGRARDWEKERQLSPPPPPPPPPRTSAPSSSPPSLESKINSFLQGNPGFSLALGDASPDGVDGTPVRDEAAGTPTQDEIMDTPGGVPESLGSSGSHNLSPTAYRNDPWDTAVTPTGSSNNGDFLGSSSSRFGAGKRSGTKLNDEDLMRKQVSSSPGGDLKARNSRALAERRISAGSRKASTGSDDASLSGKKNNGKEMPADAKEGQYHRIETLVSPHTEGAPIQTLGYSNRPSAGERIKTVESIRVIGRGSRRGGGAGSRPGASMWYEEEEYLEGQPPSPQVLPPPLSMGQGGAEDMNPSMLPLPLPPPPPHHIPHLHPPPSLPLPPPQPPFPMPYHPENMQTPPPSMLQPHLHPSAAFFATVPPIPRLPPPPSMHRLSPPPSHPSLPHSVMVGGMLVPVDHSLPLPPSLRPKGTEHSRMGPRGRKVTPPPLMSSLLGDPPKLPRPGTVKEPLIPRHTPPLHRPGAPGVPPPLLGRVKEPLVLPHPSPTSSTPSPSTPNSPAGDSSLSKPPPSPPAAPHNPGSNPVPLLNLPTSRPPILATPMQQRPPMRGRSLSQDHPRGGFHGGKRAGPPFGGGPFHAQKRPYLPPRY

Target ORF information:

RefSeq Version	XM_014987180.1
Organism	Poecilia mexicana
Definition	Poecilia mexicana threonine--tRNA ligase, cytoplasmic-like (LOC106917887), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014987180.1

ORF Insert Sequence:

ATGGCGGCTG GTTCAGGCGC TGCAAGCGGG CAGGGAGCCC GCAGCTCCAC TGGTTCTCTG 
GAAGCCTCAC TGGACCGCCG CTTTCAAGGA GTTTCCAATA CTATGGAGTC GATACAGAGC 
CTCTCGACGT GGTGCATTGA AAACAAGAAA CACCACGGCC TCATTGTTCG CTACTGGATG 
AAGTGGCTCA AGAAATCCGA CCACAATCAT CGCTTGAATC TTTTCTACCT TGCCAATGAT 
GTGATCCAAA ACTGCAAAAG AAAGAACGCC ATTGTGTTTC GTTCGGCGTT CTCCGATGTA 
CTTCCCAATG CTGCCCTCCT CATCAAAGAT GGAAAAGTCC GCAAATCGGT CGAAAGGATC 
TTTTCCATTT GGGAGGAGAG GAATGTGTAT CCCGAGGAAG TGATTGCACA GTTCAAAGCC 
AGCCTGCACA AAAAGGAAAA GGAGCGAGAA AAGCAGAAGG AGAAAGAGAA AGAAAAGCAG 
AAAGAAAAGG AGACTCCATC AAATAATGCT CCCACCAGCA CCAAAGCCGT CAGCACTAAA 
ACTCCACCTA ATAAAGCTGC GCTGAAGTCC AAGATTGTTG CAGAGTTTAC ACCCCACTCC 
CTTATCGAGC AGCTGTCGAG ACACAAGCAA GCTGCTGCTG AAGAGCAGGT CAGAGAGAAG 
CAGCTTGAGA CGCTCAGAGT GGATGTCCTC AGCACCGAGG CCTTCAAAAG GCTGAAAGAC 
AAAGCAGGAG GAAACAATTT TGCTAAGGAC TTTGAAGATG GCAGTCTGAA GCTGCAGGAG 
TTTGTCAGCT TCCTGGAGAA AGAGCTGAAA ACAGGACCTC CCCTGCTGGA AGCATTGGGA 
AATGCAGACA TCTTCTATGA GATGCAATAC AAGGAGGTTA AGATTGTGGC AAATGCGTAC 
AATGCCTTTG CCAACCGAGT AAACGGCCTC AAAAAGAAAC TGGATTCTCT CAAGGCGTCT 
CTCTCCGGCC CTGAGGACTC GCCCATCCCC TCTCCCTCTG AAGACGCTCC TTCACCCACT 
GGCTCCGACT CACCCTTTCT GGAAATGGAG ACGGCCAGGG CCAAGGTGGA TCCGGAGCTG 
GACGGCAAGG CCATGGACGA AGGAGAGCTC CAAAACGACA ACAGAGACAT GGAAGACATG 
GACATGTCTG ATGAGGAGGC ATGCGCTGGT GCTGCGCAGG ACGGTAAGTT AGACCAGCCG 
GCTGTTTCCA AGACGAAGTC GGACGAGGCG TCAAAACCTC AAAGCACGCC AACGAAACCA 
CCCAAGCCCA GCCCATCTGC TGTTACCACA GCAACCCCAG TGACTCCGAC CTCTGCTAAA 
GCTACGACAA CCCCGACCAC GCCCCTGGGA GTGAACCTGG CCAAAGTGGA CCTGGGGAAG 
ATCAGCTCCA TCCTCAACTC GCTCACGTCT GCCATGAAGA ACACAGCACC TACTGCGTCT 
CCTCGGCCGT CTCCTGGGAC CCCCACCACC CCCACCGCTC AGACAGCGGC CTCCAAAGGG 
ACGCCGCCAA GTCCTGCTTT GGCCAGCATC CTGTCACGTG TTGACATCAC CCCTGAAGGG 
ATACTAAACG CTCTGTCTAA AACCAACACA CCAGGTTTGT CATCCCTCCT TCAAAGTGTG 
ACTAATACCT CCACCGCTCC CTCCAACCGA ACCTCCACAG ACTCATCCAG TGTCAAAACC 
CCGGTAACCC CAACCACCCC GAAGACGAAG CCCCCTCTGG GGAACAGTCT CAAACAAGAC 
GAACCTGGAA GAGCCAGAGA CTGGGAGAAG GAGCGCCAGC TCTCCCCTCC ACCTCCTCCT 
CCACCCCCTC CTCGTACCTC AGCTCCTTCT TCCTCTCCCC CCAGCCTAGA ATCGAAGATC 
AACAGTTTCC TGCAGGGTAA TCCAGGCTTC AGCCTGGCGC TGGGCGACGC AAGTCCCGAT 
GGCGTCGACG GCACCCCGGT GAGGGACGAG GCAGCGGGCA CGCCGACGCA GGACGAGATC 
ATGGACACTC CTGGAGGCGT GCCAGAATCT CTGGGCTCCT CTGGAAGCCA TAACCTCTCA 
CCTACAGCCT ACCGCAATGA TCCCTGGGAT ACTGCAGTCA CTCCAACCGG AAGCAGCAAC 
AACGGAGACT TTCTTGGCTC CTCCTCCTCA AGATTCGGTG CAGGAAAAAG GAGCGGCACG 
AAGTTAAACG ACGAAGACCT GATGAGGAAG CAGGTGTCGT CTTCTCCTGG CGGTGACTTG 
AAGGCTCGGA ACAGCAGAGC CTTGGCAGAG CGGAGAATCT CTGCTGGCTC TCGGAAGGCG 
AGCACCGGTT CCGACGACGC GAGTCTGAGT GGAAAAAAGA ACAATGGGAA AGAGATGCCA 
GCAGACGCAA AGGAAGGTCA GTACCATCGC ATCGAGACAC TGGTGTCGCC CCACACCGAA 
GGAGCACCGA TCCAAACTCT GGGGTATTCA AACCGACCTT CAGCCGGAGA GCGCATCAAG 
ACAGTTGAGA GCATCCGCGT GATCGGCCGA GGTTCTCGAC GAGGGGGAGG AGCTGGGAGT 
CGGCCAGGGG CTTCGATGTG GTACGAAGAG GAAGAATACT TGGAGGGTCA GCCTCCATCA 
CCCCAAGTTC TACCCCCTCC TCTCAGCATG GGCCAGGGAG GCGCTGAAGA CATGAACCCC 
TCCATGCTTC CTCTTCCTCT TCCTCCTCCT CCACCACACC ACATCCCACA CCTTCACCCT 
CCTCCGTCTC TTCCTCTCCC TCCTCCTCAG CCTCCGTTCC CAATGCCGTA CCACCCTGAA 
AACATGCAGA CTCCTCCTCC ATCGATGCTC CAACCTCATC TCCATCCCTC TGCCGCCTTT 
TTCGCCACTG TTCCCCCAAT CCCTCGGCTG CCTCCACCTC CTTCCATGCA CCGCCTCTCA 
CCTCCGCCGT CTCACCCTTC TTTGCCCCAC TCCGTCATGG TGGGGGGGAT GCTGGTCCCT 
GTGGACCACA GCTTGCCTCT TCCTCCATCA CTAAGACCCA AAGGTACAGA GCACAGCAGA 
ATGGGGCCCA GAGGAAGGAA GGTGACCCCT CCACCACTCA TGTCATCGTT GCTAGGCGAC 
CCCCCTAAAC TACCCCGTCC CGGCACAGTT AAAGAGCCTT TAATCCCTCG CCATACCCCC 
CCTCTTCACC GCCCAGGTGC GCCTGGCGTT CCTCCGCCTT TACTCGGCAG AGTGAAGGAG 
CCTTTGGTTT TACCTCACCC CTCCCCCACA TCCTCCACGC CCTCTCCCTC CACGCCTAAT 
TCCCCCGCAG GAGACTCCTC TCTCTCCAAA CCCCCACCCA GCCCTCCAGC TGCGCCCCAC 
AACCCAGGCT CCAACCCCGT CCCCCTCCTC AACTTGCCCA CATCCAGACC CCCGATCCTC 
GCCACTCCCA TGCAGCAGAG ACCCCCGATG CGAGGCCGAA GCCTGTCTCA AGACCACCCC 
AGGGGCGGGT TTCATGGAGG GAAGCGAGCC GGACCCCCAT TCGGCGGCGG TCCCTTCCAT 
GCGCAGAAGA GGCCTTACCT TCCCCCACGC TACTGA