il6st cDNA ORF clone, Poecilia latipinna(sailfin molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	il6st
Entrez Gene ID	106937885
Full Name	interleukin 6 signal transducer
Gene Type	protein-coding
Organism	Poecilia latipinna(sailfin molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015019617.1	XP_014875103.1	interleukin-6 receptor subunit beta isoform X2
XM_015019615.1	XP_014875101.1	interleukin-6 receptor subunit beta isoform X1

Pathways from BioSystems
Gene Ontology

The following il6st gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the il6st cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo93125	XM_015019615.1 Latest version!	Poecilia latipinna interleukin 6 signal transducer (il6st), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPo93127	XM_015019617.1 Latest version!	Poecilia latipinna interleukin 6 signal transducer (il6st), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

il6st ( XM_015019615.1 ) cDNA ORF clone, Poecilia latipinna(sailfin molly) -> XP_014875101.1 Poecilia latipinna interleukin 6 signal transducer (il6st), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo93125
Clone ID Related Accession (Same CDS sequence)	XM_015019615.1
Accession Version	XM_015019615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Poecilia latipinna(sailfin molly)
Product	interleukin-6 receptor subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015115075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia latipinna Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCC TGTTTAGCAG TGGGGCTGTC GTCATGATGT CTTCGATGGT GCTCCAGTTG 
TTTGGACTGG CTTTCGTCGC CTCAGTGCTC CATGCTAAAC ACGAATTTAG CACCCATCTG 
GTGACTTCTC CTCATGTTCC AGTGGTGGAG ATCGGTACTA ACTTCACGGC TACATGCGTG 
ATCGTCAACA CAGTTGAAGT CACTGCTGAC AGTCTGTACT GGAATCTATC AGAATCATCC 
GTTGTACCTG AAAAAAACTA CATCAAGATA AATGAATCAG CACTAGCTGT GACCATCCTC 
ATCAGCGGTG AAGAATCTGA ATGGCTTTTC TGTAGCTGCA AGAAAAACTC CCCTTACGTT 
TCTCTGAATA AAAGCAAATT TATTCATGGG ATCTACCTGA AGAAAGCCTA TCCACCTCGG 
AGAGTGGAGA ACCTGTCCTG TGTGGCAGAG CAGGTTGGGA AATTGGTCTC CGGGGTTTTA 
AACTGCAAGT GGGATGCGGT TGGTGGTCAG ACTGAAGATG TCAAAACAAA CTACACAGTG 
ATGGTGCAAC ATGAGTCAGG CACATATAGA AGCAAGACAA CATTCCTCAA CAAAACCATG 
GTGGATCTGG GATTCTTCCC CCATTTCATG CATTTCGATG TTTGGGTGGT GGCAGATAAT 
TTACTGGGAA CAGTGGAGTC TGAGCATCTG AAACAAGACG CCAATGAGTT TGTGAAAACA 
GATCCTCCAT CCAAAGTGAC TGCGTTTCCA GAGGAGCTTT TCCCTAACTG CCTTTTAATC 
AAATGGGAGC ATCCTATTGA GGATGTACGA ATAAAACTGA CTTATCAAAT CCGATACTGC 
GAAAGTGGAT CCCATACCTG GAGTTATATA CCTCTTGGAG ACACCGACAA GGTCATAACT 
TCTTTCAGAC TGCAGAATCT TCAGCCAAAC AGCGCTTATG TCATTCAGAT GCGCTGCAGA 
AATCGAGAAG GGAACTCAGG TCCCTGGAGC AACTGGAGTG CCAATGAAAC TAAGACAACA 
CCAGAGGATC GACCTAGAAG TAAACCAGAT TTGTGGCAGT TGTCTGGGGG TGACCGTGGT 
GAAGAAGTGC AGATCATTTG TAAGGATCCC AAGCATGCCA ATGGAAGGAT AACATCATTC 
GGCTTAATTC TCTACAACAA GAATGGACCT TTGGAGCAGG AGATCATCCC AGCCGACCAG 
AGCCAGAACT CCAGTGAGAG CAGAGTCACT CTGCTTAAGA ACATTTCCTT GGCTGATAAA 
CAGTTTGTAA GAGTGTGCGT GAATGCCAAC AATTCCAAGG GGACTTCTCC AGAGGCGTGT 
TTGATCCTTG TCAGATCATC TGAACAAACA ACGGTGGAAA AGTTGAAGGT GTGGACCCAG 
GAGGGTCAGA TGTTTGTAGA ATGGAAACGG CCAAACAACA CCGCTGTATC TGAATATGTA 
GTTGAGTGGG CAAGGCACGG AAAAACAGAC TGGCAGAGAG AAAACAAAAC AACCACTAGG 
ACCGCAATTA AAGGGAGCCT TGAGAAGTTT GTTCGCTACA ACGTGTCAGT TTATGTGATT 
CAGTCTGGGA GGGTCTGGAA GCTGGTTCAT AAAGAAGCCT ATCTGGAACA AGGAGCTCCA 
GAACAGGGCC CTACTATTAA CAAGAGCTAC CCAGCATACG ATAATGCTCA TCTGGTGTGG 
GATGAGATTC CACTGGACAA GAGGAGGGGC TTCATCACAA ACTACAACAT ATCCTACAGA 
AAGGAGAACG AGGCCCCGAA ATCGATCCGT GTTGCAGCTA ACAACACCTC TTACACTCTG 
ACTGAGCTGC TGGGCAACAC CAAGTATGAA GTCTGGATCA GTGCTTCAAC CGTGGCAGGC 
TCAAAAAGCA GCAACCCTCA CTCATTCACC ACATTGAGAT ACGCTCCAGG AACGATTGAG 
GGCATCGTGG TAGGAGTCAG CCTGGGCTTC CTGTTCTTCG TTCTCATGGC TCTCCTGCTG 
TGCTTCCTGA AGAAGGATGT ATTAAAGGAG AACTTCTGGC CTCAGATTCC CAACCCTGGA 
GAGAGCACCA TTGGAAACTG GTCTCCTGAT TATCCTCCGA AGGAAGAGAC TCCAAAGGAG 
AACTGTGTGT CTGGTGTGTC TGTGCTTGAT GTTGGGGTGT GTGAGGTAAA GCTTGGCTTT 
GAAGAGGACA AGACCAGTCT GTCTCTGAAG AAGGGCAAAT ACCTATCTGA GGAGCACAGC 
AGCGGGATCG GCGGCTCCTC CTGCATGTCC TCGCCGCGCC AGAGCGTTTC CGACAGCGAT 
GAGGGCCCTG ACCTCGCTGA CTCCACGGCC AGCACCGTTC AGTACTCCTC TGTTGTGGCA 
TCCAGCGGTT ACAAGGGCCA GACACCCAGC TCCCATTCGC AGCATTCCAT CTTTTCGCGG 
TCCGAGTCCA CGCAGCCACT GCTGGACTCA GAGGAGCACC CAGACGTGTC GCTGCAGGAG 
GGATGCAGAC AGGTCCAGCC TCCTCCCAGA CCGTCACTGT TTACTGGTGC AGAGAACCAG 
AACAGCATGG AGTCTGATGA CTTCAGGCTG CTGGGGGTGG AGCAGCATGA CTTCTGTCCC 
CTCAGAGAGG ACACTGAGCT GACCACCGAG CCAGACAGCC TGTCTGCTGA CACAGAGTCA 
GGAGCAGTGT CCAGCTACAT GCCACAGTTG GGAGGTTACA GACCGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014875101.1
CDS	169..2859
Translation	MKLLFSSGAVVMMSSMVLQLFGLAFVASVLHAKHEFSTHLVTSPHVPVVEIGTNFTATCVIVNTVEVTADSLYWNLSESSVVPEKNYIKINESALAVTILISGEESEWLFCSCKKNSPYVSLNKSKFIHGIYLKKAYPPRRVENLSCVAEQVGKLVSGVLNCKWDAVGGQTEDVKTNYTVMVQHESGTYRSKTTFLNKTMVDLGFFPHFMHFDVWVVADNLLGTVESEHLKQDANEFVKTDPPSKVTAFPEELFPNCLLIKWEHPIEDVRIKLTYQIRYCESGSHTWSYIPLGDTDKVITSFRLQNLQPNSAYVIQMRCRNREGNSGPWSNWSANETKTTPEDRPRSKPDLWQLSGGDRGEEVQIICKDPKHANGRITSFGLILYNKNGPLEQEIIPADQSQNSSESRVTLLKNISLADKQFVRVCVNANNSKGTSPEACLILVRSSEQTTVEKLKVWTQEGQMFVEWKRPNNTAVSEYVVEWARHGKTDWQRENKTTTRTAIKGSLEKFVRYNVSVYVIQSGRVWKLVHKEAYLEQGAPEQGPTINKSYPAYDNAHLVWDEIPLDKRRGFITNYNISYRKENEAPKSIRVAANNTSYTLTELLGNTKYEVWISASTVAGSKSSNPHSFTTLRYAPGTIEGIVVGVSLGFLFFVLMALLLCFLKKDVLKENFWPQIPNPGESTIGNWSPDYPPKEETPKENCVSGVSVLDVGVCEVKLGFEEDKTSLSLKKGKYLSEEHSSGIGGSSCMSSPRQSVSDSDEGPDLADSTASTVQYSSVVASSGYKGQTPSSHSQHSIFSRSESTQPLLDSEEHPDVSLQEGCRQVQPPPRPSLFTGAENQNSMESDDFRLLGVEQHDFCPLREDTELTTEPDSLSADTESGAVSSYMPQLGGYRPQ

Target ORF information:

RefSeq Version	XM_015019615.1
Organism	Poecilia latipinna(sailfin molly)
Definition	Poecilia latipinna interleukin 6 signal transducer (il6st), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015019615.1

ORF Insert Sequence:

ATGAAGCTCC TGTTTAGCAG TGGGGCTGTC GTCATGATGT CTTCGATGGT GCTCCAGTTG 
TTTGGACTGG CTTTCGTCGC CTCAGTGCTC CATGCTAAAC ACGAATTTAG CACCCATCTG 
GTGACTTCTC CTCATGTTCC AGTGGTGGAG ATCGGTACTA ACTTCACGGC TACATGCGTG 
ATCGTCAACA CAGTTGAAGT CACTGCTGAC AGTCTGTACT GGAATCTATC AGAATCATCC 
GTTGTACCTG AAAAAAACTA CATCAAGATA AATGAATCAG CACTAGCTGT GACCATCCTC 
ATCAGCGGTG AAGAATCTGA ATGGCTTTTC TGTAGCTGCA AGAAAAACTC CCCTTACGTT 
TCTCTGAATA AAAGCAAATT TATTCATGGG ATCTACCTGA AGAAAGCCTA TCCACCTCGG 
AGAGTGGAGA ACCTGTCCTG TGTGGCAGAG CAGGTTGGGA AATTGGTCTC CGGGGTTTTA 
AACTGCAAGT GGGATGCGGT TGGTGGTCAG ACTGAAGATG TCAAAACAAA CTACACAGTG 
ATGGTGCAAC ATGAGTCAGG CACATATAGA AGCAAGACAA CATTCCTCAA CAAAACCATG 
GTGGATCTGG GATTCTTCCC CCATTTCATG CATTTCGATG TTTGGGTGGT GGCAGATAAT 
TTACTGGGAA CAGTGGAGTC TGAGCATCTG AAACAAGACG CCAATGAGTT TGTGAAAACA 
GATCCTCCAT CCAAAGTGAC TGCGTTTCCA GAGGAGCTTT TCCCTAACTG CCTTTTAATC 
AAATGGGAGC ATCCTATTGA GGATGTACGA ATAAAACTGA CTTATCAAAT CCGATACTGC 
GAAAGTGGAT CCCATACCTG GAGTTATATA CCTCTTGGAG ACACCGACAA GGTCATAACT 
TCTTTCAGAC TGCAGAATCT TCAGCCAAAC AGCGCTTATG TCATTCAGAT GCGCTGCAGA 
AATCGAGAAG GGAACTCAGG TCCCTGGAGC AACTGGAGTG CCAATGAAAC TAAGACAACA 
CCAGAGGATC GACCTAGAAG TAAACCAGAT TTGTGGCAGT TGTCTGGGGG TGACCGTGGT 
GAAGAAGTGC AGATCATTTG TAAGGATCCC AAGCATGCCA ATGGAAGGAT AACATCATTC 
GGCTTAATTC TCTACAACAA GAATGGACCT TTGGAGCAGG AGATCATCCC AGCCGACCAG 
AGCCAGAACT CCAGTGAGAG CAGAGTCACT CTGCTTAAGA ACATTTCCTT GGCTGATAAA 
CAGTTTGTAA GAGTGTGCGT GAATGCCAAC AATTCCAAGG GGACTTCTCC AGAGGCGTGT 
TTGATCCTTG TCAGATCATC TGAACAAACA ACGGTGGAAA AGTTGAAGGT GTGGACCCAG 
GAGGGTCAGA TGTTTGTAGA ATGGAAACGG CCAAACAACA CCGCTGTATC TGAATATGTA 
GTTGAGTGGG CAAGGCACGG AAAAACAGAC TGGCAGAGAG AAAACAAAAC AACCACTAGG 
ACCGCAATTA AAGGGAGCCT TGAGAAGTTT GTTCGCTACA ACGTGTCAGT TTATGTGATT 
CAGTCTGGGA GGGTCTGGAA GCTGGTTCAT AAAGAAGCCT ATCTGGAACA AGGAGCTCCA 
GAACAGGGCC CTACTATTAA CAAGAGCTAC CCAGCATACG ATAATGCTCA TCTGGTGTGG 
GATGAGATTC CACTGGACAA GAGGAGGGGC TTCATCACAA ACTACAACAT ATCCTACAGA 
AAGGAGAACG AGGCCCCGAA ATCGATCCGT GTTGCAGCTA ACAACACCTC TTACACTCTG 
ACTGAGCTGC TGGGCAACAC CAAGTATGAA GTCTGGATCA GTGCTTCAAC CGTGGCAGGC 
TCAAAAAGCA GCAACCCTCA CTCATTCACC ACATTGAGAT ACGCTCCAGG AACGATTGAG 
GGCATCGTGG TAGGAGTCAG CCTGGGCTTC CTGTTCTTCG TTCTCATGGC TCTCCTGCTG 
TGCTTCCTGA AGAAGGATGT ATTAAAGGAG AACTTCTGGC CTCAGATTCC CAACCCTGGA 
GAGAGCACCA TTGGAAACTG GTCTCCTGAT TATCCTCCGA AGGAAGAGAC TCCAAAGGAG 
AACTGTGTGT CTGGTGTGTC TGTGCTTGAT GTTGGGGTGT GTGAGGTAAA GCTTGGCTTT 
GAAGAGGACA AGACCAGTCT GTCTCTGAAG AAGGGCAAAT ACCTATCTGA GGAGCACAGC 
AGCGGGATCG GCGGCTCCTC CTGCATGTCC TCGCCGCGCC AGAGCGTTTC CGACAGCGAT 
GAGGGCCCTG ACCTCGCTGA CTCCACGGCC AGCACCGTTC AGTACTCCTC TGTTGTGGCA 
TCCAGCGGTT ACAAGGGCCA GACACCCAGC TCCCATTCGC AGCATTCCAT CTTTTCGCGG 
TCCGAGTCCA CGCAGCCACT GCTGGACTCA GAGGAGCACC CAGACGTGTC GCTGCAGGAG 
GGATGCAGAC AGGTCCAGCC TCCTCCCAGA CCGTCACTGT TTACTGGTGC AGAGAACCAG 
AACAGCATGG AGTCTGATGA CTTCAGGCTG CTGGGGGTGG AGCAGCATGA CTTCTGTCCC 
CTCAGAGAGG ACACTGAGCT GACCACCGAG CCAGACAGCC TGTCTGCTGA CACAGAGTCA 
GGAGCAGTGT CCAGCTACAT GCCACAGTTG GGAGGTTACA GACCGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

il6st ( XM_015019617.1 ) cDNA ORF clone, Poecilia latipinna(sailfin molly) -> XP_014875103.1 Poecilia latipinna interleukin 6 signal transducer (il6st), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo93127
Clone ID Related Accession (Same CDS sequence)	XM_015019617.1
Accession Version	XM_015019617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Poecilia latipinna(sailfin molly)
Product	interleukin-6 receptor subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015115075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia latipinna Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCC TGTTTAGCAG TGGGGCTGTC GTCATGATGT CTTCGATGGT GCTCCAGTTG 
TTTGGACTGG CTTTCGTCGC CTCAGTGCTC CATGCTAAAC ACGATCCACC TCGGAGAGTG 
GAGAACCTGT CCTGTGTGGC AGAGCAGGTT GGGAAATTGG TCTCCGGGGT TTTAAACTGC 
AAGTGGGATG CGGTTGGTGG TCAGACTGAA GATGTCAAAA CAAACTACAC AGTGATGGTG 
CAACATGAGT CAGGCACATA TAGAAGCAAG ACAACATTCC TCAACAAAAC CATGGTGGAT 
CTGGGATTCT TCCCCCATTT CATGCATTTC GATGTTTGGG TGGTGGCAGA TAATTTACTG 
GGAACAGTGG AGTCTGAGCA TCTGAAACAA GACGCCAATG AGTTTGTGAA AACAGATCCT 
CCATCCAAAG TGACTGCGTT TCCAGAGGAG CTTTTCCCTA ACTGCCTTTT AATCAAATGG 
GAGCATCCTA TTGAGGATGT ACGAATAAAA CTGACTTATC AAATCCGATA CTGCGAAAGT 
GGATCCCATA CCTGGAGTTA TATACCTCTT GGAGACACCG ACAAGGTCAT AACTTCTTTC 
AGACTGCAGA ATCTTCAGCC AAACAGCGCT TATGTCATTC AGATGCGCTG CAGAAATCGA 
GAAGGGAACT CAGGTCCCTG GAGCAACTGG AGTGCCAATG AAACTAAGAC AACACCAGAG 
GATCGACCTA GAAGTAAACC AGATTTGTGG CAGTTGTCTG GGGGTGACCG TGGTGAAGAA 
GTGCAGATCA TTTGTAAGGA TCCCAAGCAT GCCAATGGAA GGATAACATC ATTCGGCTTA 
ATTCTCTACA ACAAGAATGG ACCTTTGGAG CAGGAGATCA TCCCAGCCGA CCAGAGCCAG 
AACTCCAGTG AGAGCAGAGT CACTCTGCTT AAGAACATTT CCTTGGCTGA TAAACAGTTT 
GTAAGAGTGT GCGTGAATGC CAACAATTCC AAGGGGACTT CTCCAGAGGC GTGTTTGATC 
CTTGTCAGAT CATCTGAACA AACAACGGTG GAAAAGTTGA AGGTGTGGAC CCAGGAGGGT 
CAGATGTTTG TAGAATGGAA ACGGCCAAAC AACACCGCTG TATCTGAATA TGTAGTTGAG 
TGGGCAAGGC ACGGAAAAAC AGACTGGCAG AGAGAAAACA AAACAACCAC TAGGACCGCA 
ATTAAAGGGA GCCTTGAGAA GTTTGTTCGC TACAACGTGT CAGTTTATGT GATTCAGTCT 
GGGAGGGTCT GGAAGCTGGT TCATAAAGAA GCCTATCTGG AACAAGGAGC TCCAGAACAG 
GGCCCTACTA TTAACAAGAG CTACCCAGCA TACGATAATG CTCATCTGGT GTGGGATGAG 
ATTCCACTGG ACAAGAGGAG GGGCTTCATC ACAAACTACA ACATATCCTA CAGAAAGGAG 
AACGAGGCCC CGAAATCGAT CCGTGTTGCA GCTAACAACA CCTCTTACAC TCTGACTGAG 
CTGCTGGGCA ACACCAAGTA TGAAGTCTGG ATCAGTGCTT CAACCGTGGC AGGCTCAAAA 
AGCAGCAACC CTCACTCATT CACCACATTG AGATACGCTC CAGGAACGAT TGAGGGCATC 
GTGGTAGGAG TCAGCCTGGG CTTCCTGTTC TTCGTTCTCA TGGCTCTCCT GCTGTGCTTC 
CTGAAGAAGG ATGTATTAAA GGAGAACTTC TGGCCTCAGA TTCCCAACCC TGGAGAGAGC 
ACCATTGGAA ACTGGTCTCC TGATTATCCT CCGAAGGAAG AGACTCCAAA GGAGAACTGT 
GTGTCTGGTG TGTCTGTGCT TGATGTTGGG GTGTGTGAGG TAAAGCTTGG CTTTGAAGAG 
GACAAGACCA GTCTGTCTCT GAAGAAGGGC AAATACCTAT CTGAGGAGCA CAGCAGCGGG 
ATCGGCGGCT CCTCCTGCAT GTCCTCGCCG CGCCAGAGCG TTTCCGACAG CGATGAGGGC 
CCTGACCTCG CTGACTCCAC GGCCAGCACC GTTCAGTACT CCTCTGTTGT GGCATCCAGC 
GGTTACAAGG GCCAGACACC CAGCTCCCAT TCGCAGCATT CCATCTTTTC GCGGTCCGAG 
TCCACGCAGC CACTGCTGGA CTCAGAGGAG CACCCAGACG TGTCGCTGCA GGAGGGATGC 
AGACAGGTCC AGCCTCCTCC CAGACCGTCA CTGTTTACTG GTGCAGAGAA CCAGAACAGC 
ATGGAGTCTG ATGACTTCAG GCTGCTGGGG GTGGAGCAGC ATGACTTCTG TCCCCTCAGA 
GAGGACACTG AGCTGACCAC CGAGCCAGAC AGCCTGTCTG CTGACACAGA GTCAGGAGCA 
GTGTCCAGCT ACATGCCACA GTTGGGAGGT TACAGACCGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014875103.1
CDS	169..2553
Translation	MKLLFSSGAVVMMSSMVLQLFGLAFVASVLHAKHDPPRRVENLSCVAEQVGKLVSGVLNCKWDAVGGQTEDVKTNYTVMVQHESGTYRSKTTFLNKTMVDLGFFPHFMHFDVWVVADNLLGTVESEHLKQDANEFVKTDPPSKVTAFPEELFPNCLLIKWEHPIEDVRIKLTYQIRYCESGSHTWSYIPLGDTDKVITSFRLQNLQPNSAYVIQMRCRNREGNSGPWSNWSANETKTTPEDRPRSKPDLWQLSGGDRGEEVQIICKDPKHANGRITSFGLILYNKNGPLEQEIIPADQSQNSSESRVTLLKNISLADKQFVRVCVNANNSKGTSPEACLILVRSSEQTTVEKLKVWTQEGQMFVEWKRPNNTAVSEYVVEWARHGKTDWQRENKTTTRTAIKGSLEKFVRYNVSVYVIQSGRVWKLVHKEAYLEQGAPEQGPTINKSYPAYDNAHLVWDEIPLDKRRGFITNYNISYRKENEAPKSIRVAANNTSYTLTELLGNTKYEVWISASTVAGSKSSNPHSFTTLRYAPGTIEGIVVGVSLGFLFFVLMALLLCFLKKDVLKENFWPQIPNPGESTIGNWSPDYPPKEETPKENCVSGVSVLDVGVCEVKLGFEEDKTSLSLKKGKYLSEEHSSGIGGSSCMSSPRQSVSDSDEGPDLADSTASTVQYSSVVASSGYKGQTPSSHSQHSIFSRSESTQPLLDSEEHPDVSLQEGCRQVQPPPRPSLFTGAENQNSMESDDFRLLGVEQHDFCPLREDTELTTEPDSLSADTESGAVSSYMPQLGGYRPQ

Target ORF information:

RefSeq Version	XM_015019617.1
Organism	Poecilia latipinna(sailfin molly)
Definition	Poecilia latipinna interleukin 6 signal transducer (il6st), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015019617.1

ORF Insert Sequence:

ATGAAGCTCC TGTTTAGCAG TGGGGCTGTC GTCATGATGT CTTCGATGGT GCTCCAGTTG 
TTTGGACTGG CTTTCGTCGC CTCAGTGCTC CATGCTAAAC ACGATCCACC TCGGAGAGTG 
GAGAACCTGT CCTGTGTGGC AGAGCAGGTT GGGAAATTGG TCTCCGGGGT TTTAAACTGC 
AAGTGGGATG CGGTTGGTGG TCAGACTGAA GATGTCAAAA CAAACTACAC AGTGATGGTG 
CAACATGAGT CAGGCACATA TAGAAGCAAG ACAACATTCC TCAACAAAAC CATGGTGGAT 
CTGGGATTCT TCCCCCATTT CATGCATTTC GATGTTTGGG TGGTGGCAGA TAATTTACTG 
GGAACAGTGG AGTCTGAGCA TCTGAAACAA GACGCCAATG AGTTTGTGAA AACAGATCCT 
CCATCCAAAG TGACTGCGTT TCCAGAGGAG CTTTTCCCTA ACTGCCTTTT AATCAAATGG 
GAGCATCCTA TTGAGGATGT ACGAATAAAA CTGACTTATC AAATCCGATA CTGCGAAAGT 
GGATCCCATA CCTGGAGTTA TATACCTCTT GGAGACACCG ACAAGGTCAT AACTTCTTTC 
AGACTGCAGA ATCTTCAGCC AAACAGCGCT TATGTCATTC AGATGCGCTG CAGAAATCGA 
GAAGGGAACT CAGGTCCCTG GAGCAACTGG AGTGCCAATG AAACTAAGAC AACACCAGAG 
GATCGACCTA GAAGTAAACC AGATTTGTGG CAGTTGTCTG GGGGTGACCG TGGTGAAGAA 
GTGCAGATCA TTTGTAAGGA TCCCAAGCAT GCCAATGGAA GGATAACATC ATTCGGCTTA 
ATTCTCTACA ACAAGAATGG ACCTTTGGAG CAGGAGATCA TCCCAGCCGA CCAGAGCCAG 
AACTCCAGTG AGAGCAGAGT CACTCTGCTT AAGAACATTT CCTTGGCTGA TAAACAGTTT 
GTAAGAGTGT GCGTGAATGC CAACAATTCC AAGGGGACTT CTCCAGAGGC GTGTTTGATC 
CTTGTCAGAT CATCTGAACA AACAACGGTG GAAAAGTTGA AGGTGTGGAC CCAGGAGGGT 
CAGATGTTTG TAGAATGGAA ACGGCCAAAC AACACCGCTG TATCTGAATA TGTAGTTGAG 
TGGGCAAGGC ACGGAAAAAC AGACTGGCAG AGAGAAAACA AAACAACCAC TAGGACCGCA 
ATTAAAGGGA GCCTTGAGAA GTTTGTTCGC TACAACGTGT CAGTTTATGT GATTCAGTCT 
GGGAGGGTCT GGAAGCTGGT TCATAAAGAA GCCTATCTGG AACAAGGAGC TCCAGAACAG 
GGCCCTACTA TTAACAAGAG CTACCCAGCA TACGATAATG CTCATCTGGT GTGGGATGAG 
ATTCCACTGG ACAAGAGGAG GGGCTTCATC ACAAACTACA ACATATCCTA CAGAAAGGAG 
AACGAGGCCC CGAAATCGAT CCGTGTTGCA GCTAACAACA CCTCTTACAC TCTGACTGAG 
CTGCTGGGCA ACACCAAGTA TGAAGTCTGG ATCAGTGCTT CAACCGTGGC AGGCTCAAAA 
AGCAGCAACC CTCACTCATT CACCACATTG AGATACGCTC CAGGAACGAT TGAGGGCATC 
GTGGTAGGAG TCAGCCTGGG CTTCCTGTTC TTCGTTCTCA TGGCTCTCCT GCTGTGCTTC 
CTGAAGAAGG ATGTATTAAA GGAGAACTTC TGGCCTCAGA TTCCCAACCC TGGAGAGAGC 
ACCATTGGAA ACTGGTCTCC TGATTATCCT CCGAAGGAAG AGACTCCAAA GGAGAACTGT 
GTGTCTGGTG TGTCTGTGCT TGATGTTGGG GTGTGTGAGG TAAAGCTTGG CTTTGAAGAG 
GACAAGACCA GTCTGTCTCT GAAGAAGGGC AAATACCTAT CTGAGGAGCA CAGCAGCGGG 
ATCGGCGGCT CCTCCTGCAT GTCCTCGCCG CGCCAGAGCG TTTCCGACAG CGATGAGGGC 
CCTGACCTCG CTGACTCCAC GGCCAGCACC GTTCAGTACT CCTCTGTTGT GGCATCCAGC 
GGTTACAAGG GCCAGACACC CAGCTCCCAT TCGCAGCATT CCATCTTTTC GCGGTCCGAG 
TCCACGCAGC CACTGCTGGA CTCAGAGGAG CACCCAGACG TGTCGCTGCA GGAGGGATGC 
AGACAGGTCC AGCCTCCTCC CAGACCGTCA CTGTTTACTG GTGCAGAGAA CCAGAACAGC 
ATGGAGTCTG ATGACTTCAG GCTGCTGGGG GTGGAGCAGC ATGACTTCTG TCCCCTCAGA 
GAGGACACTG AGCTGACCAC CGAGCCAGAC AGCCTGTCTG CTGACACAGA GTCAGGAGCA 
GTGTCCAGCT ACATGCCACA GTTGGGAGGT TACAGACCGC AGTAA