lats1 cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	lats1
Entrez Gene ID	103154079
Full Name	large tumor suppressor kinase 1
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007575275.2	XP_007575337.1	serine/threonine-protein kinase LATS1
XM_016665718.1	XP_016521204.1	serine/threonine-protein kinase LATS1

Pathways from BioSystems
Gene Ontology

The following lats1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lats1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo126137	XM_016665718.1 Latest version!	Poecilia formosa large tumor suppressor kinase 1 (lats1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPo126137	XM_007575275.2 Latest version!	Poecilia formosa large tumor suppressor kinase 1 (lats1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lats1 ( XM_007575275.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007575337.1 Poecilia formosa large tumor suppressor kinase 1 (lats1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo126137
Clone ID Related Accession (Same CDS sequence)	XM_007575275.2 , XM_016665718.1
Accession Version	XM_007575275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	serine/threonine-protein kinase LATS1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006799940.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007575275.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAG GGGAGAAACC CGAAGGACAC AAACAGATGC GACCCAAAAC GTTCCCTGCC 
AGCAACTACA GTGGTAACAG CCAGCAGATG CTGCAGGATA TACGGAACAG CCTGCGAAAC 
CTGACCAAGC CCTCCGACCC TCCTAAAGTA GACCCTGCTG GACAAGCAAA AATGCTCCCT 
GAAGATCAAA GGCACCAGGG GCGCACCAGC ACCCCAAAAC ATTCACAACA CCACAAAGCA 
CTACAGAAGA TCCGCAAGTC TTTGGTGCCT TTTGAAGTGA CTGATTCACT GAGTAATGAT 
TCTACCACTT CTGGCCCTGA TCTCAACAAG CAGATGTTGC TGGAACAACC AACTGCTGGA 
TTTGAGGAGG GCAACAGCAG CCGTCGCGTT ACTTCAGACT ACAAGGGGAA GGTGAATTAC 
CAGGACTCGA TGAGTCCTAA CACCAACTCT ACAGGCCTGA AAGCTCCAGG TCCGTCTCAC 
ATGCAGCAGG CTGTGCTGCG GAGACCAAGT TGGAAAGGAT CCAAGGAGTC CCTCGTTCCT 
CGAATGGGTC CCCTGGTGGT GGAAGGCATT TCGTACCGCT CTGACAGCCC TGGACCGCCT 
CCCGTTTACC CACAAGGCCA CTCTGCTAGC AACCAGAGGG TGAACCCTCC GCTGCCCCCT 
CAGGTGCGGA GTGTCACGCC CCCTCCGAAC CGCAACGGCA TGCCTCCCAC GTCATCCTGG 
GAAAGCAACC CGTCCACCAA GCGGTACTCT GGGAATCTGG ACTACCTCCC TCCGCGGATG 
TCTCCGGTCC CCCAGGGCGC CCGGCCAGAC ATGTATCCAA ACCCGGCGCC TCAGAATCAG 
CGCTCTGCAC CCCATAAATT CACCTTTCCT CCCACCTGGA CTCAAAATGG CTCCGCTCAG 
CCGGACTACA TGCAGGGAGG CAACCGTCAG CCTCCGCCGC CTTACCCCGT AAACAGCCGA 
CAGGGTTCCA ACGAGCCCCA GCCTGCTTCG TCTCCTTCGT ACCTAAACGG AGGAAACATC 
CCTCAGTCCA TGCTGGTTCC CAACCGGAAC AGCCACAACC TCGACATGTA CAACCTGGCT 
CCTCCTCACA CGTGGTTGCA GCCTCCTCTG GCATCCAGTC AGCCGCACTC TGCGTCCGTC 
ACCAACATCA ACCAGGACTT GTCGCCGTCG TGGCAGCACA ACATCCCGGT TCGCTCCAAC 
TCCTTCAACA ATCGACCCAC TCATTCGTCC AGCTCCCAGC CGTCGGCGAC CACCGTCACC 
GCCATCACGC AGACGCCCAT CCTGCAGCCG GTGAAAAGCA TGCGCGTGCC GAAACCCAAG 
ATGCACACCG CCGTCGCTCC AACGCATCCA CAGCGGATAC AGCAGGTTGC ACCGTCGCCT 
CCTGCGCCTG CATATCCCGA CCCACCACCT GCGGTTTCTC AAGCGCCTAT CGGCGGGGAA 
CCCCCCAACT ATCAGGGTCC GCCGCCACCG TATCCCAAAC ACCTCTTCCC TTCGCCCGCC 
CCTCCACCGT ACAGTGCAGG CAGAGACGAG GCCACCGACG AGAGCAGCAG TGATAAGACG 
TCGGAAAGCG GCGAAAGCTC GACCGGAGCC GAAAAGAAGA TCACTACGTC GCCCGTCCCG 
GTCCGCCGCA ACAGAGACGA GGAGCGGAGA GGGGAGTCGG GAAGAATCAG CCTGTACTCC 
CCTCAAGCCT TCAAGTTCTT CATGGAGCAG CATGTAGAGA ACATCCTGAA GAACCACCAG 
ATGAGGATCC GCAGGAGGAA GCAGCTGGAA AGCGAGATGC AAAGGGTGGG TTTGAGCTCA 
GAAGCCCAGA AGCAGATGCG AAAGATGCTC TCTCAGAAGG AGTCCAACTA CATCCGCCTA 
AAGCGAGCCA AAATGGACAA GTCCATGTTC AAACGGATCA AGACACTCGG CATCGGCGCC 
TTCGGAGAAG TGTCCCTGGC GAGGAAGGAG GACACGGGGG CGCTGTACGC CATGAAGACT 
CTCCGCAAGA AGGATGTGCT GCTGAGGAAC CAGGTGGCTC ACGTAAAGGC CGAGCGGGAC 
ATCCTGGCCG AAGCCGACAA CGAGTGGGTG GTGCGTCTCT ACTACTCCTT CCAAGACAAG 
GACAACTTGT ACTTCGTCAT GGAGTACATT CCCGGCGGGG ACATGATGAG CCTCCTCATC 
CGGCTGGAAA TCTTCAAGGA AGAGCTGGCT TGGTTTTACA TCGCGGAGCT CACCTGCGCT 
TTGGAGAGCG TCCATAAGAT GGGCTTCATC CACAGAGACA TCAAGCCTGA CAACATTCTC 
ATAGACGGAG ACGGACACAT AAAGCTGACG GACTTTGGGC TCTGCACCGG TTTCCGCTGG 
ACACACGACT CCAAGTATTA CCAGAGCGGG GATCATGTGA GGCAGGACAG CATGGACTTC 
AGTAAGGATT GGGAAGATCC AGCCAACTGT CGCTGCTCCA ACCGAATGAA GCCCCTGGAG 
AGGAGAAAGG CCCGGCAGCA CCAGCGCTGC TTGGCCCACT CGCTGGTGGG AACGCCAAAC 
TACATTGCTC CAGAAGTTCT TCTTCGAACA GGATACACAC AGTTGTGTGA CTGGTGGAGT 
GTTGGTGTCA TCCTGTATGA AATGGTTGTG GGACAGCCTC CGTTCCTGGC CAACACGCCG 
CTGGAGACAC AAATGAAGGT GATAAACTGG AAGAGCACAC TGCACATCCC CCCTGAACGC 
AAGCTCAGCC CGGAGGCGTC GGACCTCATC GTCAAGCTGT GCCGCGGCCC AGAGGACCGC 
CTGGGCAAGA ACGGCGCCGA CGAAATCAAA GCTCACCCTT TCTTCAAGAG CGTCAACTTC 
TCCAGCGACC TCAGGCAGCA GGCGGCGCCG TACATACCCA CCATCGCTCA CCCGACGGAC 
ACGTCCAACT TCGACGAGGT GGAGCCAGAA AAGCTGCGCA AGAGCGACGA CGAGAGCAAC 
CACAACGACA CGCTGAACGG TTGGTTCCGC AACGGGAAGC ACCCCGAACA CGCCTTTTAC 
GAGTTCACCT TCCGCCGCTT CTTCGACGAC AACGGCCACC CCTACAGCTG CCCCAAGCCC 
ATCGAGTCCG AGGGCAGCGA AGAAGACGAG GCGGAGCTGG ACGACGCCGA GCACGACAAA 
CATGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007575337.1
CDS	327..3455
Translation	MRRGEKPEGHKQMRPKTFPASNYSGNSQQMLQDIRNSLRNLTKPSDPPKVDPAGQAKMLPEDQRHQGRTSTPKHSQHHKALQKIRKSLVPFEVTDSLSNDSTTSGPDLNKQMLLEQPTAGFEEGNSSRRVTSDYKGKVNYQDSMSPNTNSTGLKAPGPSHMQQAVLRRPSWKGSKESLVPRMGPLVVEGISYRSDSPGPPPVYPQGHSASNQRVNPPLPPQVRSVTPPPNRNGMPPTSSWESNPSTKRYSGNLDYLPPRMSPVPQGARPDMYPNPAPQNQRSAPHKFTFPPTWTQNGSAQPDYMQGGNRQPPPPYPVNSRQGSNEPQPASSPSYLNGGNIPQSMLVPNRNSHNLDMYNLAPPHTWLQPPLASSQPHSASVTNINQDLSPSWQHNIPVRSNSFNNRPTHSSSSQPSATTVTAITQTPILQPVKSMRVPKPKMHTAVAPTHPQRIQQVAPSPPAPAYPDPPPAVSQAPIGGEPPNYQGPPPPYPKHLFPSPAPPPYSAGRDEATDESSSDKTSESGESSTGAEKKITTSPVPVRRNRDEERRGESGRISLYSPQAFKFFMEQHVENILKNHQMRIRRRKQLESEMQRVGLSSEAQKQMRKMLSQKESNYIRLKRAKMDKSMFKRIKTLGIGAFGEVSLARKEDTGALYAMKTLRKKDVLLRNQVAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMEYIPGGDMMSLLIRLEIFKEELAWFYIAELTCALESVHKMGFIHRDIKPDNILIDGDGHIKLTDFGLCTGFRWTHDSKYYQSGDHVRQDSMDFSKDWEDPANCRCSNRMKPLERRKARQHQRCLAHSLVGTPNYIAPEVLLRTGYTQLCDWWSVGVILYEMVVGQPPFLANTPLETQMKVINWKSTLHIPPERKLSPEASDLIVKLCRGPEDRLGKNGADEIKAHPFFKSVNFSSDLRQQAAPYIPTIAHPTDTSNFDEVEPEKLRKSDDESNHNDTLNGWFRNGKHPEHAFYEFTFRRFFDDNGHPYSCPKPIESEGSEEDEAELDDAEHDKHG

Target ORF information:

RefSeq Version	XM_007575275.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa large tumor suppressor kinase 1 (lats1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007575275.2

ORF Insert Sequence:

ATGAGGAGAG GGGAGAAACC CGAAGGACAC AAACAGATGC GACCCAAAAC GTTCCCTGCC 
AGCAACTACA GTGGTAACAG CCAGCAGATG CTGCAGGATA TACGGAACAG CCTGCGAAAC 
CTGACCAAGC CCTCCGACCC TCCTAAAGTA GACCCTGCTG GACAAGCAAA AATGCTCCCT 
GAAGATCAAA GGCACCAGGG GCGCACCAGC ACCCCAAAAC ATTCACAACA CCACAAAGCA 
CTACAGAAGA TCCGCAAGTC TTTGGTGCCT TTTGAAGTGA CTGATTCACT GAGTAATGAT 
TCTACCACTT CTGGCCCTGA TCTCAACAAG CAGATGTTGC TGGAACAACC AACTGCTGGA 
TTTGAGGAGG GCAACAGCAG CCGTCGCGTT ACTTCAGACT ACAAGGGGAA GGTGAATTAC 
CAGGACTCGA TGAGTCCTAA CACCAACTCT ACAGGCCTGA AAGCTCCAGG TCCGTCTCAC 
ATGCAGCAGG CTGTGCTGCG GAGACCAAGT TGGAAAGGAT CCAAGGAGTC CCTCGTTCCT 
CGAATGGGTC CCCTGGTGGT GGAAGGCATT TCGTACCGCT CTGACAGCCC TGGACCGCCT 
CCCGTTTACC CACAAGGCCA CTCTGCTAGC AACCAGAGGG TGAACCCTCC GCTGCCCCCT 
CAGGTGCGGA GTGTCACGCC CCCTCCGAAC CGCAACGGCA TGCCTCCCAC GTCATCCTGG 
GAAAGCAACC CGTCCACCAA GCGGTACTCT GGGAATCTGG ACTACCTCCC TCCGCGGATG 
TCTCCGGTCC CCCAGGGCGC CCGGCCAGAC ATGTATCCAA ACCCGGCGCC TCAGAATCAG 
CGCTCTGCAC CCCATAAATT CACCTTTCCT CCCACCTGGA CTCAAAATGG CTCCGCTCAG 
CCGGACTACA TGCAGGGAGG CAACCGTCAG CCTCCGCCGC CTTACCCCGT AAACAGCCGA 
CAGGGTTCCA ACGAGCCCCA GCCTGCTTCG TCTCCTTCGT ACCTAAACGG AGGAAACATC 
CCTCAGTCCA TGCTGGTTCC CAACCGGAAC AGCCACAACC TCGACATGTA CAACCTGGCT 
CCTCCTCACA CGTGGTTGCA GCCTCCTCTG GCATCCAGTC AGCCGCACTC TGCGTCCGTC 
ACCAACATCA ACCAGGACTT GTCGCCGTCG TGGCAGCACA ACATCCCGGT TCGCTCCAAC 
TCCTTCAACA ATCGACCCAC TCATTCGTCC AGCTCCCAGC CGTCGGCGAC CACCGTCACC 
GCCATCACGC AGACGCCCAT CCTGCAGCCG GTGAAAAGCA TGCGCGTGCC GAAACCCAAG 
ATGCACACCG CCGTCGCTCC AACGCATCCA CAGCGGATAC AGCAGGTTGC ACCGTCGCCT 
CCTGCGCCTG CATATCCCGA CCCACCACCT GCGGTTTCTC AAGCGCCTAT CGGCGGGGAA 
CCCCCCAACT ATCAGGGTCC GCCGCCACCG TATCCCAAAC ACCTCTTCCC TTCGCCCGCC 
CCTCCACCGT ACAGTGCAGG CAGAGACGAG GCCACCGACG AGAGCAGCAG TGATAAGACG 
TCGGAAAGCG GCGAAAGCTC GACCGGAGCC GAAAAGAAGA TCACTACGTC GCCCGTCCCG 
GTCCGCCGCA ACAGAGACGA GGAGCGGAGA GGGGAGTCGG GAAGAATCAG CCTGTACTCC 
CCTCAAGCCT TCAAGTTCTT CATGGAGCAG CATGTAGAGA ACATCCTGAA GAACCACCAG 
ATGAGGATCC GCAGGAGGAA GCAGCTGGAA AGCGAGATGC AAAGGGTGGG TTTGAGCTCA 
GAAGCCCAGA AGCAGATGCG AAAGATGCTC TCTCAGAAGG AGTCCAACTA CATCCGCCTA 
AAGCGAGCCA AAATGGACAA GTCCATGTTC AAACGGATCA AGACACTCGG CATCGGCGCC 
TTCGGAGAAG TGTCCCTGGC GAGGAAGGAG GACACGGGGG CGCTGTACGC CATGAAGACT 
CTCCGCAAGA AGGATGTGCT GCTGAGGAAC CAGGTGGCTC ACGTAAAGGC CGAGCGGGAC 
ATCCTGGCCG AAGCCGACAA CGAGTGGGTG GTGCGTCTCT ACTACTCCTT CCAAGACAAG 
GACAACTTGT ACTTCGTCAT GGAGTACATT CCCGGCGGGG ACATGATGAG CCTCCTCATC 
CGGCTGGAAA TCTTCAAGGA AGAGCTGGCT TGGTTTTACA TCGCGGAGCT CACCTGCGCT 
TTGGAGAGCG TCCATAAGAT GGGCTTCATC CACAGAGACA TCAAGCCTGA CAACATTCTC 
ATAGACGGAG ACGGACACAT AAAGCTGACG GACTTTGGGC TCTGCACCGG TTTCCGCTGG 
ACACACGACT CCAAGTATTA CCAGAGCGGG GATCATGTGA GGCAGGACAG CATGGACTTC 
AGTAAGGATT GGGAAGATCC AGCCAACTGT CGCTGCTCCA ACCGAATGAA GCCCCTGGAG 
AGGAGAAAGG CCCGGCAGCA CCAGCGCTGC TTGGCCCACT CGCTGGTGGG AACGCCAAAC 
TACATTGCTC CAGAAGTTCT TCTTCGAACA GGATACACAC AGTTGTGTGA CTGGTGGAGT 
GTTGGTGTCA TCCTGTATGA AATGGTTGTG GGACAGCCTC CGTTCCTGGC CAACACGCCG 
CTGGAGACAC AAATGAAGGT GATAAACTGG AAGAGCACAC TGCACATCCC CCCTGAACGC 
AAGCTCAGCC CGGAGGCGTC GGACCTCATC GTCAAGCTGT GCCGCGGCCC AGAGGACCGC 
CTGGGCAAGA ACGGCGCCGA CGAAATCAAA GCTCACCCTT TCTTCAAGAG CGTCAACTTC 
TCCAGCGACC TCAGGCAGCA GGCGGCGCCG TACATACCCA CCATCGCTCA CCCGACGGAC 
ACGTCCAACT TCGACGAGGT GGAGCCAGAA AAGCTGCGCA AGAGCGACGA CGAGAGCAAC 
CACAACGACA CGCTGAACGG TTGGTTCCGC AACGGGAAGC ACCCCGAACA CGCCTTTTAC 
GAGTTCACCT TCCGCCGCTT CTTCGACGAC AACGGCCACC CCTACAGCTG CCCCAAGCCC 
ATCGAGTCCG AGGGCAGCGA AGAAGACGAG GCGGAGCTGG ACGACGCCGA GCACGACAAA 
CATGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lats1 ( XM_016665718.1 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_016521204.1 Poecilia formosa large tumor suppressor kinase 1 (lats1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo126137
Clone ID Related Accession (Same CDS sequence)	XM_007575275.2 , XM_016665718.1
Accession Version	XM_016665718.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	serine/threonine-protein kinase LATS1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006799940.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAG GGGAGAAACC CGAAGGACAC AAACAGATGC GACCCAAAAC GTTCCCTGCC 
AGCAACTACA GTGGTAACAG CCAGCAGATG CTGCAGGATA TACGGAACAG CCTGCGAAAC 
CTGACCAAGC CCTCCGACCC TCCTAAAGTA GACCCTGCTG GACAAGCAAA AATGCTCCCT 
GAAGATCAAA GGCACCAGGG GCGCACCAGC ACCCCAAAAC ATTCACAACA CCACAAAGCA 
CTACAGAAGA TCCGCAAGTC TTTGGTGCCT TTTGAAGTGA CTGATTCACT GAGTAATGAT 
TCTACCACTT CTGGCCCTGA TCTCAACAAG CAGATGTTGC TGGAACAACC AACTGCTGGA 
TTTGAGGAGG GCAACAGCAG CCGTCGCGTT ACTTCAGACT ACAAGGGGAA GGTGAATTAC 
CAGGACTCGA TGAGTCCTAA CACCAACTCT ACAGGCCTGA AAGCTCCAGG TCCGTCTCAC 
ATGCAGCAGG CTGTGCTGCG GAGACCAAGT TGGAAAGGAT CCAAGGAGTC CCTCGTTCCT 
CGAATGGGTC CCCTGGTGGT GGAAGGCATT TCGTACCGCT CTGACAGCCC TGGACCGCCT 
CCCGTTTACC CACAAGGCCA CTCTGCTAGC AACCAGAGGG TGAACCCTCC GCTGCCCCCT 
CAGGTGCGGA GTGTCACGCC CCCTCCGAAC CGCAACGGCA TGCCTCCCAC GTCATCCTGG 
GAAAGCAACC CGTCCACCAA GCGGTACTCT GGGAATCTGG ACTACCTCCC TCCGCGGATG 
TCTCCGGTCC CCCAGGGCGC CCGGCCAGAC ATGTATCCAA ACCCGGCGCC TCAGAATCAG 
CGCTCTGCAC CCCATAAATT CACCTTTCCT CCCACCTGGA CTCAAAATGG CTCCGCTCAG 
CCGGACTACA TGCAGGGAGG CAACCGTCAG CCTCCGCCGC CTTACCCCGT AAACAGCCGA 
CAGGGTTCCA ACGAGCCCCA GCCTGCTTCG TCTCCTTCGT ACCTAAACGG AGGAAACATC 
CCTCAGTCCA TGCTGGTTCC CAACCGGAAC AGCCACAACC TCGACATGTA CAACCTGGCT 
CCTCCTCACA CGTGGTTGCA GCCTCCTCTG GCATCCAGTC AGCCGCACTC TGCGTCCGTC 
ACCAACATCA ACCAGGACTT GTCGCCGTCG TGGCAGCACA ACATCCCGGT TCGCTCCAAC 
TCCTTCAACA ATCGACCCAC TCATTCGTCC AGCTCCCAGC CGTCGGCGAC CACCGTCACC 
GCCATCACGC AGACGCCCAT CCTGCAGCCG GTGAAAAGCA TGCGCGTGCC GAAACCCAAG 
ATGCACACCG CCGTCGCTCC AACGCATCCA CAGCGGATAC AGCAGGTTGC ACCGTCGCCT 
CCTGCGCCTG CATATCCCGA CCCACCACCT GCGGTTTCTC AAGCGCCTAT CGGCGGGGAA 
CCCCCCAACT ATCAGGGTCC GCCGCCACCG TATCCCAAAC ACCTCTTCCC TTCGCCCGCC 
CCTCCACCGT ACAGTGCAGG CAGAGACGAG GCCACCGACG AGAGCAGCAG TGATAAGACG 
TCGGAAAGCG GCGAAAGCTC GACCGGAGCC GAAAAGAAGA TCACTACGTC GCCCGTCCCG 
GTCCGCCGCA ACAGAGACGA GGAGCGGAGA GGGGAGTCGG GAAGAATCAG CCTGTACTCC 
CCTCAAGCCT TCAAGTTCTT CATGGAGCAG CATGTAGAGA ACATCCTGAA GAACCACCAG 
ATGAGGATCC GCAGGAGGAA GCAGCTGGAA AGCGAGATGC AAAGGGTGGG TTTGAGCTCA 
GAAGCCCAGA AGCAGATGCG AAAGATGCTC TCTCAGAAGG AGTCCAACTA CATCCGCCTA 
AAGCGAGCCA AAATGGACAA GTCCATGTTC AAACGGATCA AGACACTCGG CATCGGCGCC 
TTCGGAGAAG TGTCCCTGGC GAGGAAGGAG GACACGGGGG CGCTGTACGC CATGAAGACT 
CTCCGCAAGA AGGATGTGCT GCTGAGGAAC CAGGTGGCTC ACGTAAAGGC CGAGCGGGAC 
ATCCTGGCCG AAGCCGACAA CGAGTGGGTG GTGCGTCTCT ACTACTCCTT CCAAGACAAG 
GACAACTTGT ACTTCGTCAT GGAGTACATT CCCGGCGGGG ACATGATGAG CCTCCTCATC 
CGGCTGGAAA TCTTCAAGGA AGAGCTGGCT TGGTTTTACA TCGCGGAGCT CACCTGCGCT 
TTGGAGAGCG TCCATAAGAT GGGCTTCATC CACAGAGACA TCAAGCCTGA CAACATTCTC 
ATAGACGGAG ACGGACACAT AAAGCTGACG GACTTTGGGC TCTGCACCGG TTTCCGCTGG 
ACACACGACT CCAAGTATTA CCAGAGCGGG GATCATGTGA GGCAGGACAG CATGGACTTC 
AGTAAGGATT GGGAAGATCC AGCCAACTGT CGCTGCTCCA ACCGAATGAA GCCCCTGGAG 
AGGAGAAAGG CCCGGCAGCA CCAGCGCTGC TTGGCCCACT CGCTGGTGGG AACGCCAAAC 
TACATTGCTC CAGAAGTTCT TCTTCGAACA GGATACACAC AGTTGTGTGA CTGGTGGAGT 
GTTGGTGTCA TCCTGTATGA AATGGTTGTG GGACAGCCTC CGTTCCTGGC CAACACGCCG 
CTGGAGACAC AAATGAAGGT GATAAACTGG AAGAGCACAC TGCACATCCC CCCTGAACGC 
AAGCTCAGCC CGGAGGCGTC GGACCTCATC GTCAAGCTGT GCCGCGGCCC AGAGGACCGC 
CTGGGCAAGA ACGGCGCCGA CGAAATCAAA GCTCACCCTT TCTTCAAGAG CGTCAACTTC 
TCCAGCGACC TCAGGCAGCA GGCGGCGCCG TACATACCCA CCATCGCTCA CCCGACGGAC 
ACGTCCAACT TCGACGAGGT GGAGCCAGAA AAGCTGCGCA AGAGCGACGA CGAGAGCAAC 
CACAACGACA CGCTGAACGG TTGGTTCCGC AACGGGAAGC ACCCCGAACA CGCCTTTTAC 
GAGTTCACCT TCCGCCGCTT CTTCGACGAC AACGGCCACC CCTACAGCTG CCCCAAGCCC 
ATCGAGTCCG AGGGCAGCGA AGAAGACGAG GCGGAGCTGG ACGACGCCGA GCACGACAAA 
CATGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016521204.1
CDS	324..3452
Translation	MRRGEKPEGHKQMRPKTFPASNYSGNSQQMLQDIRNSLRNLTKPSDPPKVDPAGQAKMLPEDQRHQGRTSTPKHSQHHKALQKIRKSLVPFEVTDSLSNDSTTSGPDLNKQMLLEQPTAGFEEGNSSRRVTSDYKGKVNYQDSMSPNTNSTGLKAPGPSHMQQAVLRRPSWKGSKESLVPRMGPLVVEGISYRSDSPGPPPVYPQGHSASNQRVNPPLPPQVRSVTPPPNRNGMPPTSSWESNPSTKRYSGNLDYLPPRMSPVPQGARPDMYPNPAPQNQRSAPHKFTFPPTWTQNGSAQPDYMQGGNRQPPPPYPVNSRQGSNEPQPASSPSYLNGGNIPQSMLVPNRNSHNLDMYNLAPPHTWLQPPLASSQPHSASVTNINQDLSPSWQHNIPVRSNSFNNRPTHSSSSQPSATTVTAITQTPILQPVKSMRVPKPKMHTAVAPTHPQRIQQVAPSPPAPAYPDPPPAVSQAPIGGEPPNYQGPPPPYPKHLFPSPAPPPYSAGRDEATDESSSDKTSESGESSTGAEKKITTSPVPVRRNRDEERRGESGRISLYSPQAFKFFMEQHVENILKNHQMRIRRRKQLESEMQRVGLSSEAQKQMRKMLSQKESNYIRLKRAKMDKSMFKRIKTLGIGAFGEVSLARKEDTGALYAMKTLRKKDVLLRNQVAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMEYIPGGDMMSLLIRLEIFKEELAWFYIAELTCALESVHKMGFIHRDIKPDNILIDGDGHIKLTDFGLCTGFRWTHDSKYYQSGDHVRQDSMDFSKDWEDPANCRCSNRMKPLERRKARQHQRCLAHSLVGTPNYIAPEVLLRTGYTQLCDWWSVGVILYEMVVGQPPFLANTPLETQMKVINWKSTLHIPPERKLSPEASDLIVKLCRGPEDRLGKNGADEIKAHPFFKSVNFSSDLRQQAAPYIPTIAHPTDTSNFDEVEPEKLRKSDDESNHNDTLNGWFRNGKHPEHAFYEFTFRRFFDDNGHPYSCPKPIESEGSEEDEAELDDAEHDKHG

Target ORF information:

RefSeq Version	XM_016665718.1
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa large tumor suppressor kinase 1 (lats1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016665718.1

ORF Insert Sequence:

ATGAGGAGAG GGGAGAAACC CGAAGGACAC AAACAGATGC GACCCAAAAC GTTCCCTGCC 
AGCAACTACA GTGGTAACAG CCAGCAGATG CTGCAGGATA TACGGAACAG CCTGCGAAAC 
CTGACCAAGC CCTCCGACCC TCCTAAAGTA GACCCTGCTG GACAAGCAAA AATGCTCCCT 
GAAGATCAAA GGCACCAGGG GCGCACCAGC ACCCCAAAAC ATTCACAACA CCACAAAGCA 
CTACAGAAGA TCCGCAAGTC TTTGGTGCCT TTTGAAGTGA CTGATTCACT GAGTAATGAT 
TCTACCACTT CTGGCCCTGA TCTCAACAAG CAGATGTTGC TGGAACAACC AACTGCTGGA 
TTTGAGGAGG GCAACAGCAG CCGTCGCGTT ACTTCAGACT ACAAGGGGAA GGTGAATTAC 
CAGGACTCGA TGAGTCCTAA CACCAACTCT ACAGGCCTGA AAGCTCCAGG TCCGTCTCAC 
ATGCAGCAGG CTGTGCTGCG GAGACCAAGT TGGAAAGGAT CCAAGGAGTC CCTCGTTCCT 
CGAATGGGTC CCCTGGTGGT GGAAGGCATT TCGTACCGCT CTGACAGCCC TGGACCGCCT 
CCCGTTTACC CACAAGGCCA CTCTGCTAGC AACCAGAGGG TGAACCCTCC GCTGCCCCCT 
CAGGTGCGGA GTGTCACGCC CCCTCCGAAC CGCAACGGCA TGCCTCCCAC GTCATCCTGG 
GAAAGCAACC CGTCCACCAA GCGGTACTCT GGGAATCTGG ACTACCTCCC TCCGCGGATG 
TCTCCGGTCC CCCAGGGCGC CCGGCCAGAC ATGTATCCAA ACCCGGCGCC TCAGAATCAG 
CGCTCTGCAC CCCATAAATT CACCTTTCCT CCCACCTGGA CTCAAAATGG CTCCGCTCAG 
CCGGACTACA TGCAGGGAGG CAACCGTCAG CCTCCGCCGC CTTACCCCGT AAACAGCCGA 
CAGGGTTCCA ACGAGCCCCA GCCTGCTTCG TCTCCTTCGT ACCTAAACGG AGGAAACATC 
CCTCAGTCCA TGCTGGTTCC CAACCGGAAC AGCCACAACC TCGACATGTA CAACCTGGCT 
CCTCCTCACA CGTGGTTGCA GCCTCCTCTG GCATCCAGTC AGCCGCACTC TGCGTCCGTC 
ACCAACATCA ACCAGGACTT GTCGCCGTCG TGGCAGCACA ACATCCCGGT TCGCTCCAAC 
TCCTTCAACA ATCGACCCAC TCATTCGTCC AGCTCCCAGC CGTCGGCGAC CACCGTCACC 
GCCATCACGC AGACGCCCAT CCTGCAGCCG GTGAAAAGCA TGCGCGTGCC GAAACCCAAG 
ATGCACACCG CCGTCGCTCC AACGCATCCA CAGCGGATAC AGCAGGTTGC ACCGTCGCCT 
CCTGCGCCTG CATATCCCGA CCCACCACCT GCGGTTTCTC AAGCGCCTAT CGGCGGGGAA 
CCCCCCAACT ATCAGGGTCC GCCGCCACCG TATCCCAAAC ACCTCTTCCC TTCGCCCGCC 
CCTCCACCGT ACAGTGCAGG CAGAGACGAG GCCACCGACG AGAGCAGCAG TGATAAGACG 
TCGGAAAGCG GCGAAAGCTC GACCGGAGCC GAAAAGAAGA TCACTACGTC GCCCGTCCCG 
GTCCGCCGCA ACAGAGACGA GGAGCGGAGA GGGGAGTCGG GAAGAATCAG CCTGTACTCC 
CCTCAAGCCT TCAAGTTCTT CATGGAGCAG CATGTAGAGA ACATCCTGAA GAACCACCAG 
ATGAGGATCC GCAGGAGGAA GCAGCTGGAA AGCGAGATGC AAAGGGTGGG TTTGAGCTCA 
GAAGCCCAGA AGCAGATGCG AAAGATGCTC TCTCAGAAGG AGTCCAACTA CATCCGCCTA 
AAGCGAGCCA AAATGGACAA GTCCATGTTC AAACGGATCA AGACACTCGG CATCGGCGCC 
TTCGGAGAAG TGTCCCTGGC GAGGAAGGAG GACACGGGGG CGCTGTACGC CATGAAGACT 
CTCCGCAAGA AGGATGTGCT GCTGAGGAAC CAGGTGGCTC ACGTAAAGGC CGAGCGGGAC 
ATCCTGGCCG AAGCCGACAA CGAGTGGGTG GTGCGTCTCT ACTACTCCTT CCAAGACAAG 
GACAACTTGT ACTTCGTCAT GGAGTACATT CCCGGCGGGG ACATGATGAG CCTCCTCATC 
CGGCTGGAAA TCTTCAAGGA AGAGCTGGCT TGGTTTTACA TCGCGGAGCT CACCTGCGCT 
TTGGAGAGCG TCCATAAGAT GGGCTTCATC CACAGAGACA TCAAGCCTGA CAACATTCTC 
ATAGACGGAG ACGGACACAT AAAGCTGACG GACTTTGGGC TCTGCACCGG TTTCCGCTGG 
ACACACGACT CCAAGTATTA CCAGAGCGGG GATCATGTGA GGCAGGACAG CATGGACTTC 
AGTAAGGATT GGGAAGATCC AGCCAACTGT CGCTGCTCCA ACCGAATGAA GCCCCTGGAG 
AGGAGAAAGG CCCGGCAGCA CCAGCGCTGC TTGGCCCACT CGCTGGTGGG AACGCCAAAC 
TACATTGCTC CAGAAGTTCT TCTTCGAACA GGATACACAC AGTTGTGTGA CTGGTGGAGT 
GTTGGTGTCA TCCTGTATGA AATGGTTGTG GGACAGCCTC CGTTCCTGGC CAACACGCCG 
CTGGAGACAC AAATGAAGGT GATAAACTGG AAGAGCACAC TGCACATCCC CCCTGAACGC 
AAGCTCAGCC CGGAGGCGTC GGACCTCATC GTCAAGCTGT GCCGCGGCCC AGAGGACCGC 
CTGGGCAAGA ACGGCGCCGA CGAAATCAAA GCTCACCCTT TCTTCAAGAG CGTCAACTTC 
TCCAGCGACC TCAGGCAGCA GGCGGCGCCG TACATACCCA CCATCGCTCA CCCGACGGAC 
ACGTCCAACT TCGACGAGGT GGAGCCAGAA AAGCTGCGCA AGAGCGACGA CGAGAGCAAC 
CACAACGACA CGCTGAACGG TTGGTTCCGC AACGGGAAGC ACCCCGAACA CGCCTTTTAC 
GAGTTCACCT TCCGCCGCTT CTTCGACGAC AACGGCCACC CCTACAGCTG CCCCAAGCCC 
ATCGAGTCCG AGGGCAGCGA AGAAGACGAG GCGGAGCTGG ACGACGCCGA GCACGACAAA 
CATGGCTGA