bckdk cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bckdk
Entrez Gene ID	103145532
Full Name	branched chain ketoacid dehydrogenase kinase
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007562982.2	XP_007563044.1	3-methyl-2-oxobutanoate dehydrogenase [lipoamide] kinase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following bckdk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bckdk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo145824	XM_007562982.2 Latest version!	Poecilia formosa branched chain ketoacid dehydrogenase kinase (bckdk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bckdk ( XM_007562982.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007563044.1 Poecilia formosa branched chain ketoacid dehydrogenase kinase (bckdk), mRNA.

Price & Availability↑

CloneID	OPo145824
Clone ID Related Accession (Same CDS sequence)	XM_007562982.2
Accession Version	XM_007562982.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	3-methyl-2-oxobutanoate dehydrogenase [lipoamide] kinase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006800082.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007562982.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACCGG GACTGGCAGG CATAGCGGCG GAGATGCTGA GCGGTGTCAC CGGCAGGGCC 
AGGCCGAGAA TCGGCAGCCT CTTACTGACT CCCGCAACCA AAACATTACC GGCGCTGCAG 
TCAGGCACCG GGAATCACAG ATTCCGGTCC ACATCATCAG CACAGGGTGT CTCAGAACTG 
GCAAGAGAGC GGTCGAAGAC TGTCACTTCC TTTTACAACC AGTCCGCCAT CGATGTTTCG 
GCAGAGAAGG CCTCAGTGCG GCTGACCTTA GCGACCCTCC TGTATTCTGG GAAGTCTCCT 
GATGGACACC ACATCTTGAG CAGTGCCAAG TACCTTCACA AGGAGTTGCC TGTGCGGATC 
GCCCATCGCA TCAAGGGCTT CCGCAGTCTG CCCTTCATTA TCGGTTGCAA CCCGACCATT 
CTTCAAGTGC ATGAGCTGTA CATCAGAGCC TACCACATGC TCAGCGACTT TCCCCAGATC 
AAGGACCAGG AGGTCGAGGC TCGTTTCTGT AAACTGGTGC AGCAGCTGCT GGACGACCAC 
AAAGACGTGG TGACCATGCT GGCCCAGGGC TTCAGGGAGT GTCGACGACA CATCCAGGAT 
GAGACCATCA TCAGGAACTT CTTGGATACG ACGCTGTGTT CCCGTCTGGG AATCCGAATG 
TTAGCCACAC ACCATCTGGC TCTTCATGAA GAAAATGCGG ATTTTGTGGG GATCATTTGC 
AGACGCCTTT CTCCTAAAAA GATCATCGAG AAGTGGGTGG ACTTTTCCAG GCGTCTGTGT 
GAACACCAGT ACGGTAACTC TCCCCGGGTC CGGATCAACG GACACGTGGC GGCCCGGTTC 
CCCTTCATCC CGCTGCCGCT GGACTACATC CTGCCAGAGC TGCTCAAGAA CGCCATGAGA 
GCCACCATGG AGAGCCACCT GGACACTCCG TACAACGTCC CCGATGTGGT CGTCACCATC 
GCCAACAACG ACATCGACTT TGTCATCAGG ATTTCAGATC GTGGCGGCGG CATCCCTCAC 
AAAATAATCG ACAAGGTGAT GGATTACCAC TTCAGCACTG CCGAGGAGAG CGTGCAGGAC 
CCCCGCATGA GCAACCTGTT CAACACCATC ACTAACAGCG GGAACCAGTC CAGCCCAATG 
CACGGATTTG GTTTCGGCCT GCCCACATCC AGAGCCTACG CAGAGTACCT CGGCGGCTCC 
CTGTCTGTAC AGTCCATGCA GGGCATCGGC ACCGACGTCT ACCTGCGTCT GCGCCACATC 
GATGGCAAAG GAGAAAGCTT CAGAGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007563044.1
CDS	158..1447
Translation	MRPGLAGIAAEMLSGVTGRARPRIGSLLLTPATKTLPALQSGTGNHRFRSTSSAQGVSELARERSKTVTSFYNQSAIDVSAEKASVRLTLATLLYSGKSPDGHHILSSAKYLHKELPVRIAHRIKGFRSLPFIIGCNPTILQVHELYIRAYHMLSDFPQIKDQEVEARFCKLVQQLLDDHKDVVTMLAQGFRECRRHIQDETIIRNFLDTTLCSRLGIRMLATHHLALHEENADFVGIICRRLSPKKIIEKWVDFSRRLCEHQYGNSPRVRINGHVAARFPFIPLPLDYILPELLKNAMRATMESHLDTPYNVPDVVVTIANNDIDFVIRISDRGGGIPHKIIDKVMDYHFSTAEESVQDPRMSNLFNTITNSGNQSSPMHGFGFGLPTSRAYAEYLGGSLSVQSMQGIGTDVYLRLRHIDGKGESFRV

Target ORF information:

RefSeq Version	XM_007562982.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa branched chain ketoacid dehydrogenase kinase (bckdk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007562982.2

ORF Insert Sequence:

ATGCGACCGG GACTGGCAGG CATAGCGGCG GAGATGCTGA GCGGTGTCAC CGGCAGGGCC 
AGGCCGAGAA TCGGCAGCCT CTTACTGACT CCCGCAACCA AAACATTACC GGCGCTGCAG 
TCAGGCACCG GGAATCACAG ATTCCGGTCC ACATCATCAG CACAGGGTGT CTCAGAACTG 
GCAAGAGAGC GGTCGAAGAC TGTCACTTCC TTTTACAACC AGTCCGCCAT CGATGTTTCG 
GCAGAGAAGG CCTCAGTGCG GCTGACCTTA GCGACCCTCC TGTATTCTGG GAAGTCTCCT 
GATGGACACC ACATCTTGAG CAGTGCCAAG TACCTTCACA AGGAGTTGCC TGTGCGGATC 
GCCCATCGCA TCAAGGGCTT CCGCAGTCTG CCCTTCATTA TCGGTTGCAA CCCGACCATT 
CTTCAAGTGC ATGAGCTGTA CATCAGAGCC TACCACATGC TCAGCGACTT TCCCCAGATC 
AAGGACCAGG AGGTCGAGGC TCGTTTCTGT AAACTGGTGC AGCAGCTGCT GGACGACCAC 
AAAGACGTGG TGACCATGCT GGCCCAGGGC TTCAGGGAGT GTCGACGACA CATCCAGGAT 
GAGACCATCA TCAGGAACTT CTTGGATACG ACGCTGTGTT CCCGTCTGGG AATCCGAATG 
TTAGCCACAC ACCATCTGGC TCTTCATGAA GAAAATGCGG ATTTTGTGGG GATCATTTGC 
AGACGCCTTT CTCCTAAAAA GATCATCGAG AAGTGGGTGG ACTTTTCCAG GCGTCTGTGT 
GAACACCAGT ACGGTAACTC TCCCCGGGTC CGGATCAACG GACACGTGGC GGCCCGGTTC 
CCCTTCATCC CGCTGCCGCT GGACTACATC CTGCCAGAGC TGCTCAAGAA CGCCATGAGA 
GCCACCATGG AGAGCCACCT GGACACTCCG TACAACGTCC CCGATGTGGT CGTCACCATC 
GCCAACAACG ACATCGACTT TGTCATCAGG ATTTCAGATC GTGGCGGCGG CATCCCTCAC 
AAAATAATCG ACAAGGTGAT GGATTACCAC TTCAGCACTG CCGAGGAGAG CGTGCAGGAC 
CCCCGCATGA GCAACCTGTT CAACACCATC ACTAACAGCG GGAACCAGTC CAGCCCAATG 
CACGGATTTG GTTTCGGCCT GCCCACATCC AGAGCCTACG CAGAGTACCT CGGCGGCTCC 
CTGTCTGTAC AGTCCATGCA GGGCATCGGC ACCGACGTCT ACCTGCGTCT GCGCCACATC 
GATGGCAAAG GAGAAAGCTT CAGAGTGTAA