wdr60 cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wdr60
Entrez Gene ID	103140466
Full Name	WD repeat domain 60
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007555638.2	XP_007555700.1	WD repeat-containing protein 60 isoform X2
XM_016673192.1	XP_016528678.1	WD repeat-containing protein 60 isoform X1

Pathways from BioSystems
Gene Ontology

The following wdr60 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wdr60 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo139309	XM_016673192.1 Latest version!	Poecilia formosa WD repeat domain 60 (wdr60), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPo139310	XM_007555638.2 Latest version!	Poecilia formosa WD repeat domain 60 (wdr60), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wdr60 ( XM_016673192.1 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_016528678.1 Poecilia formosa WD repeat domain 60 (wdr60), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo139309
Clone ID Related Accession (Same CDS sequence)	XM_016673192.1
Accession Version	XM_016673192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	WD repeat-containing protein 60 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006800010.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAGTG ATAAGAAATT AACTAAGGAA GACACATGGA GACCTGCTGA GCTGAGAAGA 
CACCTCAGGG AAAAAGACTA TGGGGATGGC TATTCAAGAC GGAGAGAAGA CAATGAGAGA 
AAGCATCGTG GTGGAGAGTC CGTAGAGAGA CGGCACAGAG ATCCAGAGGA GTCCAGACGA 
GACAGAGACG AGCTCAAGGA AAGAGAGAGA CGACATGGAG ACAGTAGAGA CAGACATGCA 
GAGCGAAGGA AGGACGGGTC TCGTGACAGG AGAGGTGACA AAGACAGGGA GAGACGAGAT 
GACGAGGACA GGAGAAAACG GGTAGTTGAT GGTAGAGATA TCAGGGACAG ACACAAACAT 
AAGGAGAAAG AGGAACTAAA AGTAAGGGAG GAGGTCAGGG ACAACAGGAG AGAAGAAAGA 
CTAAAGGAAA GAAGAGAGGA ACTGGAAAGG GAGCTCAGGA GAGAGAGAAG GCGGCATGAT 
GAAAGGGGTG AAGCAGGTAG AGATCAGGCA GAAAAACTGC ACAGAGAGCT GAGGGACAGG 
CAGCACAGAG GCGAATATGA AGACAAGCGA AGAGACAAAG ATGTTCAAGA AGAAAGAAGG 
CGTTCAGAGC GAATAGAAGA TGGGGCGCGT AAAGGAGACC ACAGACGACT TAAAGAGGAA 
GGAGAAAGAA GACACAAGGA GAGAAGACAG CATGTAGATG AGAAACAGAA ACACAAAAGT 
GAATCCAAGG AACGTCAAGA CTTGAAAAAA TCTGACAGCG ATGTCCGGAG CTCCGACGCT 
GACAGGGATC GAACAGAAAG GGAAAAGCAC AAAGTAAGAC ATAAAAAAGA GGAGGACAGT 
GCCGATGGAA GGCATAAAGA CAGACGACAC AGGGAGGCAG AGGAAAAGGA CGTGAAGAGA 
AGCAGTTCCA AGAAATCCTC ATCGGACAAA AGGAAGGAAG AGAAAAAGGA TGAGAAAGAG 
CAGCAGCAGA GAGCAGATGG ACAACAAAGA GAGGACCGTA TAGAAAATGC AGACATGGCA 
GAGCAAGAAT ATGAAGAGGA TTTTGAGGAT TATGAAGAGG ACTTTGAAGA CATGCACGAG 
AGTGCTGATG AAGGCGAGGA CGATGATGAA GGTGAGGACA ATGATGGGGA AGAGGTGCAG 
ACTGCAGAAC AAAGTAAAGA GATTGAGGCG ATCCGGAGAG CCATGGCTGA GGAGAACGAG 
AGAGTTGGAA CGGCTCAGTC CAGGAGCAGT ACGGCCAGAG AGGAGGAGCA GAACAGGCCC 
AAATGGTCCA AAGGCTCTGA AAGGAATGAG AGTAAAACTT CTCGCCATGG CAAATTTATC 
AACTTCGTGG CTGCAAAGCA ACGTGAAATC AGCAAGAAGG TTGCCACAAA GCAGATGAAG 
CGCAGTACAG AGCTGCTTCG TTTAATCGAT CTGGACTTTT CCATGACGTT CTCCCTCCTC 
GACATCCCTC CTGTCAGTGA ATATGAGATG TATATAAAGA GCTTTGGAAA TGCCAACACC 
CAGCAGGCTT ACGTTCAGTG TAACGAGGAT AACGCAGACC GATACGTCCA GACAGAGGAA 
GTAGAAATAT GTGAAAAGTG GACTCAGCAT CCCCCTGAAT ACAATGGAGC ATGTGGAGAT 
CCAAACTTCT CTCAGGATGC ACAAGACAAA AGCAGGACTG ACCTCCTTCT CGATTCACAA 
TGTCTGATGT CGTTTGTCCG CTCAGCCTCA CAGGTCATGG TGGTTCTACT GGAGGAAGAT 
CAGGCAGAGA GGAAATCCCT AAGAAAGCCG AAGACTAAAA CAGATTCGCT TTCTTTCAGC 
GATGGAAGTT TGCAACTCAA CACTAAGTTG CCTTTTCTTT TAGGTCGCCA GATTAGTTTG 
GTGCAGTTCT CTCAGGTTCA GAAGCACATC ATGCTGTCCG TCCACATGCC CACATCCAAA 
GCCAGCACTG CCGGCCTCGA CGGCTGCACC GTCATCTGTG TCTGGAACAT CTGGGAGCCG 
TCCAGACCTC AGAAGATACT CATCTATGAA TCAGAGGTCC AGTGTTGCTG CTTCAGTCCA 
GGAAAGTCCA CTCTGGTGTT TGCAGGGACA CAGGTTGGTT CTGTGGTGCT GTGGGACCTG 
AGGGAGTATG CCAGCAACCA TTACAGATTA AAGATTGGTG ATCATGAGTG GACCCTCAGA 
CATCCCACCT TTTCCACCGA CGCAGTGATG TCCGGCTCAG GCCATGTTTC TCCTGTCACC 
TCCGTAGAGG TCGTTCCCTC CTTTGTTGCT GGAGGACTGA GACCAGAGGT CGCACTTCTA 
GCCTCAGAGG AAGAATCATC AGGATTGTCG TTCCAGCTTG CCTCCTTGGA TGAAAACGGG 
ATTTTAAATT TCTGGGTGGT GGTGGAGCTC CCTAGAGGCA ACGAGGCAGG CTCGCCGACA 
GATTTAGGTC TCCGTCCCGG GGGAAGAGTG AAGCTGCTTC ACAGTTCCTC CCTCCTCACT 
GTTCAGAGGC CGTTACTAAA AGAGGCAGCA AAAACAAGGC CCTCTCAGAC GCTGCTTTTA 
AAATACCATC CGACAGACTC CAATCATTTC TTTATTGGCA CCAATCTGGG TCTGGTCAGA 
CATGGAACAA GTCACGGTTT GAAGGCTCCT CCGCAGTTTT ACAGGTCCCA GGAGATGGAA 
GAAAGAACTG TTGACATCAA CTCAATCCAC TTCTCTCCTT TCAGACCAAA TCTCTTCCTG 
GTGGGCTGTG CAGACGGCAG CATCAGGATG CATGCAGTCA GCCACGATCA GCCCGTGGCA 
GAATGGATGA ACGGCACCGG TGGAGAAGCG GTGGTCTCGC TGCAGTGGAC CCAGACCAGG 
CCTGCTGTGT TTTGTGTCCT CGACGCCGCT TCTAACCTTC ACATATGGGA CATGTTGAAA 
GATGATGGAC AGCCTGTTAT GACGGAGACA TTTCGTGATG ATAGGGTAAC AGCTATGGCT 
GCGTTTGGAG ACTCGGGACA ACAAAACACA TACTCAGGAA TTGCGCTGGC ACACAAGTCA 
GGAAATATAG AGCTGCAGTA TTTCACTCCG TCTTTGACGT TGCCCAACCC TGCAGAGGAG 
GGAAAGTTGG AGAGCATAAT GACTGAAGCC TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016528678.1
CDS	142..3237
Translation	MHSDKKLTKEDTWRPAELRRHLREKDYGDGYSRRREDNERKHRGGESVERRHRDPEESRRDRDELKERERRHGDSRDRHAERRKDGSRDRRGDKDRERRDDEDRRKRVVDGRDIRDRHKHKEKEELKVREEVRDNRREERLKERREELERELRRERRRHDERGEAGRDQAEKLHRELRDRQHRGEYEDKRRDKDVQEERRRSERIEDGARKGDHRRLKEEGERRHKERRQHVDEKQKHKSESKERQDLKKSDSDVRSSDADRDRTEREKHKVRHKKEEDSADGRHKDRRHREAEEKDVKRSSSKKSSSDKRKEEKKDEKEQQQRADGQQREDRIENADMAEQEYEEDFEDYEEDFEDMHESADEGEDDDEGEDNDGEEVQTAEQSKEIEAIRRAMAEENERVGTAQSRSSTAREEEQNRPKWSKGSERNESKTSRHGKFINFVAAKQREISKKVATKQMKRSTELLRLIDLDFSMTFSLLDIPPVSEYEMYIKSFGNANTQQAYVQCNEDNADRYVQTEEVEICEKWTQHPPEYNGACGDPNFSQDAQDKSRTDLLLDSQCLMSFVRSASQVMVVLLEEDQAERKSLRKPKTKTDSLSFSDGSLQLNTKLPFLLGRQISLVQFSQVQKHIMLSVHMPTSKASTAGLDGCTVICVWNIWEPSRPQKILIYESEVQCCCFSPGKSTLVFAGTQVGSVVLWDLREYASNHYRLKIGDHEWTLRHPTFSTDAVMSGSGHVSPVTSVEVVPSFVAGGLRPEVALLASEEESSGLSFQLASLDENGILNFWVVVELPRGNEAGSPTDLGLRPGGRVKLLHSSSLLTVQRPLLKEAAKTRPSQTLLLKYHPTDSNHFFIGTNLGLVRHGTSHGLKAPPQFYRSQEMEERTVDINSIHFSPFRPNLFLVGCADGSIRMHAVSHDQPVAEWMNGTGGEAVVSLQWTQTRPAVFCVLDAASNLHIWDMLKDDGQPVMTETFRDDRVTAMAAFGDSGQQNTYSGIALAHKSGNIELQYFTPSLTLPNPAEEGKLESIMTEAF

Target ORF information:

RefSeq Version	XM_016673192.1
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa WD repeat domain 60 (wdr60), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016673192.1

ORF Insert Sequence:

ATGCACAGTG ATAAGAAATT AACTAAGGAA GACACATGGA GACCTGCTGA GCTGAGAAGA 
CACCTCAGGG AAAAAGACTA TGGGGATGGC TATTCAAGAC GGAGAGAAGA CAATGAGAGA 
AAGCATCGTG GTGGAGAGTC CGTAGAGAGA CGGCACAGAG ATCCAGAGGA GTCCAGACGA 
GACAGAGACG AGCTCAAGGA AAGAGAGAGA CGACATGGAG ACAGTAGAGA CAGACATGCA 
GAGCGAAGGA AGGACGGGTC TCGTGACAGG AGAGGTGACA AAGACAGGGA GAGACGAGAT 
GACGAGGACA GGAGAAAACG GGTAGTTGAT GGTAGAGATA TCAGGGACAG ACACAAACAT 
AAGGAGAAAG AGGAACTAAA AGTAAGGGAG GAGGTCAGGG ACAACAGGAG AGAAGAAAGA 
CTAAAGGAAA GAAGAGAGGA ACTGGAAAGG GAGCTCAGGA GAGAGAGAAG GCGGCATGAT 
GAAAGGGGTG AAGCAGGTAG AGATCAGGCA GAAAAACTGC ACAGAGAGCT GAGGGACAGG 
CAGCACAGAG GCGAATATGA AGACAAGCGA AGAGACAAAG ATGTTCAAGA AGAAAGAAGG 
CGTTCAGAGC GAATAGAAGA TGGGGCGCGT AAAGGAGACC ACAGACGACT TAAAGAGGAA 
GGAGAAAGAA GACACAAGGA GAGAAGACAG CATGTAGATG AGAAACAGAA ACACAAAAGT 
GAATCCAAGG AACGTCAAGA CTTGAAAAAA TCTGACAGCG ATGTCCGGAG CTCCGACGCT 
GACAGGGATC GAACAGAAAG GGAAAAGCAC AAAGTAAGAC ATAAAAAAGA GGAGGACAGT 
GCCGATGGAA GGCATAAAGA CAGACGACAC AGGGAGGCAG AGGAAAAGGA CGTGAAGAGA 
AGCAGTTCCA AGAAATCCTC ATCGGACAAA AGGAAGGAAG AGAAAAAGGA TGAGAAAGAG 
CAGCAGCAGA GAGCAGATGG ACAACAAAGA GAGGACCGTA TAGAAAATGC AGACATGGCA 
GAGCAAGAAT ATGAAGAGGA TTTTGAGGAT TATGAAGAGG ACTTTGAAGA CATGCACGAG 
AGTGCTGATG AAGGCGAGGA CGATGATGAA GGTGAGGACA ATGATGGGGA AGAGGTGCAG 
ACTGCAGAAC AAAGTAAAGA GATTGAGGCG ATCCGGAGAG CCATGGCTGA GGAGAACGAG 
AGAGTTGGAA CGGCTCAGTC CAGGAGCAGT ACGGCCAGAG AGGAGGAGCA GAACAGGCCC 
AAATGGTCCA AAGGCTCTGA AAGGAATGAG AGTAAAACTT CTCGCCATGG CAAATTTATC 
AACTTCGTGG CTGCAAAGCA ACGTGAAATC AGCAAGAAGG TTGCCACAAA GCAGATGAAG 
CGCAGTACAG AGCTGCTTCG TTTAATCGAT CTGGACTTTT CCATGACGTT CTCCCTCCTC 
GACATCCCTC CTGTCAGTGA ATATGAGATG TATATAAAGA GCTTTGGAAA TGCCAACACC 
CAGCAGGCTT ACGTTCAGTG TAACGAGGAT AACGCAGACC GATACGTCCA GACAGAGGAA 
GTAGAAATAT GTGAAAAGTG GACTCAGCAT CCCCCTGAAT ACAATGGAGC ATGTGGAGAT 
CCAAACTTCT CTCAGGATGC ACAAGACAAA AGCAGGACTG ACCTCCTTCT CGATTCACAA 
TGTCTGATGT CGTTTGTCCG CTCAGCCTCA CAGGTCATGG TGGTTCTACT GGAGGAAGAT 
CAGGCAGAGA GGAAATCCCT AAGAAAGCCG AAGACTAAAA CAGATTCGCT TTCTTTCAGC 
GATGGAAGTT TGCAACTCAA CACTAAGTTG CCTTTTCTTT TAGGTCGCCA GATTAGTTTG 
GTGCAGTTCT CTCAGGTTCA GAAGCACATC ATGCTGTCCG TCCACATGCC CACATCCAAA 
GCCAGCACTG CCGGCCTCGA CGGCTGCACC GTCATCTGTG TCTGGAACAT CTGGGAGCCG 
TCCAGACCTC AGAAGATACT CATCTATGAA TCAGAGGTCC AGTGTTGCTG CTTCAGTCCA 
GGAAAGTCCA CTCTGGTGTT TGCAGGGACA CAGGTTGGTT CTGTGGTGCT GTGGGACCTG 
AGGGAGTATG CCAGCAACCA TTACAGATTA AAGATTGGTG ATCATGAGTG GACCCTCAGA 
CATCCCACCT TTTCCACCGA CGCAGTGATG TCCGGCTCAG GCCATGTTTC TCCTGTCACC 
TCCGTAGAGG TCGTTCCCTC CTTTGTTGCT GGAGGACTGA GACCAGAGGT CGCACTTCTA 
GCCTCAGAGG AAGAATCATC AGGATTGTCG TTCCAGCTTG CCTCCTTGGA TGAAAACGGG 
ATTTTAAATT TCTGGGTGGT GGTGGAGCTC CCTAGAGGCA ACGAGGCAGG CTCGCCGACA 
GATTTAGGTC TCCGTCCCGG GGGAAGAGTG AAGCTGCTTC ACAGTTCCTC CCTCCTCACT 
GTTCAGAGGC CGTTACTAAA AGAGGCAGCA AAAACAAGGC CCTCTCAGAC GCTGCTTTTA 
AAATACCATC CGACAGACTC CAATCATTTC TTTATTGGCA CCAATCTGGG TCTGGTCAGA 
CATGGAACAA GTCACGGTTT GAAGGCTCCT CCGCAGTTTT ACAGGTCCCA GGAGATGGAA 
GAAAGAACTG TTGACATCAA CTCAATCCAC TTCTCTCCTT TCAGACCAAA TCTCTTCCTG 
GTGGGCTGTG CAGACGGCAG CATCAGGATG CATGCAGTCA GCCACGATCA GCCCGTGGCA 
GAATGGATGA ACGGCACCGG TGGAGAAGCG GTGGTCTCGC TGCAGTGGAC CCAGACCAGG 
CCTGCTGTGT TTTGTGTCCT CGACGCCGCT TCTAACCTTC ACATATGGGA CATGTTGAAA 
GATGATGGAC AGCCTGTTAT GACGGAGACA TTTCGTGATG ATAGGGTAAC AGCTATGGCT 
GCGTTTGGAG ACTCGGGACA ACAAAACACA TACTCAGGAA TTGCGCTGGC ACACAAGTCA 
GGAAATATAG AGCTGCAGTA TTTCACTCCG TCTTTGACGT TGCCCAACCC TGCAGAGGAG 
GGAAAGTTGG AGAGCATAAT GACTGAAGCC TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wdr60 ( XM_007555638.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007555700.1 Poecilia formosa WD repeat domain 60 (wdr60), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo139310
Clone ID Related Accession (Same CDS sequence)	XM_007555638.2
Accession Version	XM_007555638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	WD repeat-containing protein 60 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006800010.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007555638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAGTG ATAAGAAATT AACTAAGGAA GACACATGGA GACCTGCTGA GCTGAGAAGA 
CACCTCAGGG AAAAAGACTA TGGGGATGGC TATTCAAGAC GGAGAGAAGA CAATGAGAGA 
AAGCATCGTG GTGGAGAGTC CGTAGAGAGA CGGCACAGAG ATCCAGAGGA GTCCAGACGA 
GACAGAGACG AGCTCAAGGA AAGAGAGAGA CGACATGGAG ACAGTAGAGA CAGACATGCA 
GAGCGAAGGA AGGACGGGTC TCGTGACAGG AGAGGTGACA AAGACAGGGA GAGACGAGAT 
GACGAGGACA GGAGAAAACG GGTAGTTGAT GGTAGAGATA TCAGGGACAG ACACAAACAT 
AAGGAGAAAG AGGAACTAAA AGTAAGGGAG GAGGTCAGGG ACAACAGGAG AGAAGAAAGA 
CTAAAGGAAA GAAGAGAGGA ACTGGAAAGG GAGCTCAGGA GAGAGAGAAG GCGGCATGAT 
GAAAGGGGTG AAGCAGGTAG AGATCAGGCA GAAAAACTGC ACAGAGAGCT GAGGGACAGG 
CAGCACAGAG GCGAATATGA AGACAAGCGA AGAGACAAAG ATGTTCAAGA AGAAAGAAGG 
CGTTCAGAGC GAATAGAAGA TGGGGCGCGT AAAGGAGACC ACAGACGACT TAAAGAGGAA 
GGAGAAAGAA GACACAAGGA GAGAAGACAG CATGATGAGA AACAGAAACA CAAAAGTGAA 
TCCAAGGAAC GTCAAGACTT GAAAAAATCT GACAGCGATG TCCGGAGCTC CGACGCTGAC 
AGGGATCGAA CAGAAAGGGA AAAGCACAAA GTAAGACATA AAAAAGAGGA GGACAGTGCC 
GATGGAAGGC ATAAAGACAG ACGACACAGG GAGGCAGAGG AAAAGGACGT GAAGAGAAGC 
AGTTCCAAGA AATCCTCATC GGACAAAAGG AAGGAAGAGA AAAAGGATGA GAAAGAGCAG 
CAGCAGAGAG CAGATGGACA ACAAAGAGAG GACCGTATAG AAAATGCAGA CATGGCAGAG 
CAAGAATATG AAGAGGATTT TGAGGATTAT GAAGAGGACT TTGAAGACAT GCACGAGAGT 
GCTGATGAAG GCGAGGACGA TGATGAAGGT GAGGACAATG ATGGGGAAGA GGTGCAGACT 
GCAGAACAAA GTAAAGAGAT TGAGGCGATC CGGAGAGCCA TGGCTGAGGA GAACGAGAGA 
GTTGGAACGG CTCAGTCCAG GAGCAGTACG GCCAGAGAGG AGGAGCAGAA CAGGCCCAAA 
TGGTCCAAAG GCTCTGAAAG GAATGAGAGT AAAACTTCTC GCCATGGCAA ATTTATCAAC 
TTCGTGGCTG CAAAGCAACG TGAAATCAGC AAGAAGGTTG CCACAAAGCA GATGAAGCGC 
AGTACAGAGC TGCTTCGTTT AATCGATCTG GACTTTTCCA TGACGTTCTC CCTCCTCGAC 
ATCCCTCCTG TCAGTGAATA TGAGATGTAT ATAAAGAGCT TTGGAAATGC CAACACCCAG 
CAGGCTTACG TTCAGTGTAA CGAGGATAAC GCAGACCGAT ACGTCCAGAC AGAGGAAGTA 
GAAATATGTG AAAAGTGGAC TCAGCATCCC CCTGAATACA ATGGAGCATG TGGAGATCCA 
AACTTCTCTC AGGATGCACA AGACAAAAGC AGGACTGACC TCCTTCTCGA TTCACAATGT 
CTGATGTCGT TTGTCCGCTC AGCCTCACAG GTCATGGTGG TTCTACTGGA GGAAGATCAG 
GCAGAGAGGA AATCCCTAAG AAAGCCGAAG ACTAAAACAG ATTCGCTTTC TTTCAGCGAT 
GGAAGTTTGC AACTCAACAC TAAGTTGCCT TTTCTTTTAG GTCGCCAGAT TAGTTTGGTG 
CAGTTCTCTC AGGTTCAGAA GCACATCATG CTGTCCGTCC ACATGCCCAC ATCCAAAGCC 
AGCACTGCCG GCCTCGACGG CTGCACCGTC ATCTGTGTCT GGAACATCTG GGAGCCGTCC 
AGACCTCAGA AGATACTCAT CTATGAATCA GAGGTCCAGT GTTGCTGCTT CAGTCCAGGA 
AAGTCCACTC TGGTGTTTGC AGGGACACAG GTTGGTTCTG TGGTGCTGTG GGACCTGAGG 
GAGTATGCCA GCAACCATTA CAGATTAAAG ATTGGTGATC ATGAGTGGAC CCTCAGACAT 
CCCACCTTTT CCACCGACGC AGTGATGTCC GGCTCAGGCC ATGTTTCTCC TGTCACCTCC 
GTAGAGGTCG TTCCCTCCTT TGTTGCTGGA GGACTGAGAC CAGAGGTCGC ACTTCTAGCC 
TCAGAGGAAG AATCATCAGG ATTGTCGTTC CAGCTTGCCT CCTTGGATGA AAACGGGATT 
TTAAATTTCT GGGTGGTGGT GGAGCTCCCT AGAGGCAACG AGGCAGGCTC GCCGACAGAT 
TTAGGTCTCC GTCCCGGGGG AAGAGTGAAG CTGCTTCACA GTTCCTCCCT CCTCACTGTT 
CAGAGGCCGT TACTAAAAGA GGCAGCAAAA ACAAGGCCCT CTCAGACGCT GCTTTTAAAA 
TACCATCCGA CAGACTCCAA TCATTTCTTT ATTGGCACCA ATCTGGGTCT GGTCAGACAT 
GGAACAAGTC ACGGTTTGAA GGCTCCTCCG CAGTTTTACA GGTCCCAGGA GATGGAAGAA 
AGAACTGTTG ACATCAACTC AATCCACTTC TCTCCTTTCA GACCAAATCT CTTCCTGGTG 
GGCTGTGCAG ACGGCAGCAT CAGGATGCAT GCAGTCAGCC ACGATCAGCC CGTGGCAGAA 
TGGATGAACG GCACCGGTGG AGAAGCGGTG GTCTCGCTGC AGTGGACCCA GACCAGGCCT 
GCTGTGTTTT GTGTCCTCGA CGCCGCTTCT AACCTTCACA TATGGGACAT GTTGAAAGAT 
GATGGACAGC CTGTTATGAC GGAGACATTT CGTGATGATA GGGTAACAGC TATGGCTGCG 
TTTGGAGACT CGGGACAACA AAACACATAC TCAGGAATTG CGCTGGCACA CAAGTCAGGA 
AATATAGAGC TGCAGTATTT CACTCCGTCT TTGACGTTGC CCAACCCTGC AGAGGAGGGA 
AAGTTGGAGA GCATAATGAC TGAAGCCTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007555700.1
CDS	141..3233
Translation	MHSDKKLTKEDTWRPAELRRHLREKDYGDGYSRRREDNERKHRGGESVERRHRDPEESRRDRDELKERERRHGDSRDRHAERRKDGSRDRRGDKDRERRDDEDRRKRVVDGRDIRDRHKHKEKEELKVREEVRDNRREERLKERREELERELRRERRRHDERGEAGRDQAEKLHRELRDRQHRGEYEDKRRDKDVQEERRRSERIEDGARKGDHRRLKEEGERRHKERRQHDEKQKHKSESKERQDLKKSDSDVRSSDADRDRTEREKHKVRHKKEEDSADGRHKDRRHREAEEKDVKRSSSKKSSSDKRKEEKKDEKEQQQRADGQQREDRIENADMAEQEYEEDFEDYEEDFEDMHESADEGEDDDEGEDNDGEEVQTAEQSKEIEAIRRAMAEENERVGTAQSRSSTAREEEQNRPKWSKGSERNESKTSRHGKFINFVAAKQREISKKVATKQMKRSTELLRLIDLDFSMTFSLLDIPPVSEYEMYIKSFGNANTQQAYVQCNEDNADRYVQTEEVEICEKWTQHPPEYNGACGDPNFSQDAQDKSRTDLLLDSQCLMSFVRSASQVMVVLLEEDQAERKSLRKPKTKTDSLSFSDGSLQLNTKLPFLLGRQISLVQFSQVQKHIMLSVHMPTSKASTAGLDGCTVICVWNIWEPSRPQKILIYESEVQCCCFSPGKSTLVFAGTQVGSVVLWDLREYASNHYRLKIGDHEWTLRHPTFSTDAVMSGSGHVSPVTSVEVVPSFVAGGLRPEVALLASEEESSGLSFQLASLDENGILNFWVVVELPRGNEAGSPTDLGLRPGGRVKLLHSSSLLTVQRPLLKEAAKTRPSQTLLLKYHPTDSNHFFIGTNLGLVRHGTSHGLKAPPQFYRSQEMEERTVDINSIHFSPFRPNLFLVGCADGSIRMHAVSHDQPVAEWMNGTGGEAVVSLQWTQTRPAVFCVLDAASNLHIWDMLKDDGQPVMTETFRDDRVTAMAAFGDSGQQNTYSGIALAHKSGNIELQYFTPSLTLPNPAEEGKLESIMTEAF

Target ORF information:

RefSeq Version	XM_007555638.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa WD repeat domain 60 (wdr60), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007555638.2

ORF Insert Sequence:

ATGCACAGTG ATAAGAAATT AACTAAGGAA GACACATGGA GACCTGCTGA GCTGAGAAGA 
CACCTCAGGG AAAAAGACTA TGGGGATGGC TATTCAAGAC GGAGAGAAGA CAATGAGAGA 
AAGCATCGTG GTGGAGAGTC CGTAGAGAGA CGGCACAGAG ATCCAGAGGA GTCCAGACGA 
GACAGAGACG AGCTCAAGGA AAGAGAGAGA CGACATGGAG ACAGTAGAGA CAGACATGCA 
GAGCGAAGGA AGGACGGGTC TCGTGACAGG AGAGGTGACA AAGACAGGGA GAGACGAGAT 
GACGAGGACA GGAGAAAACG GGTAGTTGAT GGTAGAGATA TCAGGGACAG ACACAAACAT 
AAGGAGAAAG AGGAACTAAA AGTAAGGGAG GAGGTCAGGG ACAACAGGAG AGAAGAAAGA 
CTAAAGGAAA GAAGAGAGGA ACTGGAAAGG GAGCTCAGGA GAGAGAGAAG GCGGCATGAT 
GAAAGGGGTG AAGCAGGTAG AGATCAGGCA GAAAAACTGC ACAGAGAGCT GAGGGACAGG 
CAGCACAGAG GCGAATATGA AGACAAGCGA AGAGACAAAG ATGTTCAAGA AGAAAGAAGG 
CGTTCAGAGC GAATAGAAGA TGGGGCGCGT AAAGGAGACC ACAGACGACT TAAAGAGGAA 
GGAGAAAGAA GACACAAGGA GAGAAGACAG CATGATGAGA AACAGAAACA CAAAAGTGAA 
TCCAAGGAAC GTCAAGACTT GAAAAAATCT GACAGCGATG TCCGGAGCTC CGACGCTGAC 
AGGGATCGAA CAGAAAGGGA AAAGCACAAA GTAAGACATA AAAAAGAGGA GGACAGTGCC 
GATGGAAGGC ATAAAGACAG ACGACACAGG GAGGCAGAGG AAAAGGACGT GAAGAGAAGC 
AGTTCCAAGA AATCCTCATC GGACAAAAGG AAGGAAGAGA AAAAGGATGA GAAAGAGCAG 
CAGCAGAGAG CAGATGGACA ACAAAGAGAG GACCGTATAG AAAATGCAGA CATGGCAGAG 
CAAGAATATG AAGAGGATTT TGAGGATTAT GAAGAGGACT TTGAAGACAT GCACGAGAGT 
GCTGATGAAG GCGAGGACGA TGATGAAGGT GAGGACAATG ATGGGGAAGA GGTGCAGACT 
GCAGAACAAA GTAAAGAGAT TGAGGCGATC CGGAGAGCCA TGGCTGAGGA GAACGAGAGA 
GTTGGAACGG CTCAGTCCAG GAGCAGTACG GCCAGAGAGG AGGAGCAGAA CAGGCCCAAA 
TGGTCCAAAG GCTCTGAAAG GAATGAGAGT AAAACTTCTC GCCATGGCAA ATTTATCAAC 
TTCGTGGCTG CAAAGCAACG TGAAATCAGC AAGAAGGTTG CCACAAAGCA GATGAAGCGC 
AGTACAGAGC TGCTTCGTTT AATCGATCTG GACTTTTCCA TGACGTTCTC CCTCCTCGAC 
ATCCCTCCTG TCAGTGAATA TGAGATGTAT ATAAAGAGCT TTGGAAATGC CAACACCCAG 
CAGGCTTACG TTCAGTGTAA CGAGGATAAC GCAGACCGAT ACGTCCAGAC AGAGGAAGTA 
GAAATATGTG AAAAGTGGAC TCAGCATCCC CCTGAATACA ATGGAGCATG TGGAGATCCA 
AACTTCTCTC AGGATGCACA AGACAAAAGC AGGACTGACC TCCTTCTCGA TTCACAATGT 
CTGATGTCGT TTGTCCGCTC AGCCTCACAG GTCATGGTGG TTCTACTGGA GGAAGATCAG 
GCAGAGAGGA AATCCCTAAG AAAGCCGAAG ACTAAAACAG ATTCGCTTTC TTTCAGCGAT 
GGAAGTTTGC AACTCAACAC TAAGTTGCCT TTTCTTTTAG GTCGCCAGAT TAGTTTGGTG 
CAGTTCTCTC AGGTTCAGAA GCACATCATG CTGTCCGTCC ACATGCCCAC ATCCAAAGCC 
AGCACTGCCG GCCTCGACGG CTGCACCGTC ATCTGTGTCT GGAACATCTG GGAGCCGTCC 
AGACCTCAGA AGATACTCAT CTATGAATCA GAGGTCCAGT GTTGCTGCTT CAGTCCAGGA 
AAGTCCACTC TGGTGTTTGC AGGGACACAG GTTGGTTCTG TGGTGCTGTG GGACCTGAGG 
GAGTATGCCA GCAACCATTA CAGATTAAAG ATTGGTGATC ATGAGTGGAC CCTCAGACAT 
CCCACCTTTT CCACCGACGC AGTGATGTCC GGCTCAGGCC ATGTTTCTCC TGTCACCTCC 
GTAGAGGTCG TTCCCTCCTT TGTTGCTGGA GGACTGAGAC CAGAGGTCGC ACTTCTAGCC 
TCAGAGGAAG AATCATCAGG ATTGTCGTTC CAGCTTGCCT CCTTGGATGA AAACGGGATT 
TTAAATTTCT GGGTGGTGGT GGAGCTCCCT AGAGGCAACG AGGCAGGCTC GCCGACAGAT 
TTAGGTCTCC GTCCCGGGGG AAGAGTGAAG CTGCTTCACA GTTCCTCCCT CCTCACTGTT 
CAGAGGCCGT TACTAAAAGA GGCAGCAAAA ACAAGGCCCT CTCAGACGCT GCTTTTAAAA 
TACCATCCGA CAGACTCCAA TCATTTCTTT ATTGGCACCA ATCTGGGTCT GGTCAGACAT 
GGAACAAGTC ACGGTTTGAA GGCTCCTCCG CAGTTTTACA GGTCCCAGGA GATGGAAGAA 
AGAACTGTTG ACATCAACTC AATCCACTTC TCTCCTTTCA GACCAAATCT CTTCCTGGTG 
GGCTGTGCAG ACGGCAGCAT CAGGATGCAT GCAGTCAGCC ACGATCAGCC CGTGGCAGAA 
TGGATGAACG GCACCGGTGG AGAAGCGGTG GTCTCGCTGC AGTGGACCCA GACCAGGCCT 
GCTGTGTTTT GTGTCCTCGA CGCCGCTTCT AACCTTCACA TATGGGACAT GTTGAAAGAT 
GATGGACAGC CTGTTATGAC GGAGACATTT CGTGATGATA GGGTAACAGC TATGGCTGCG 
TTTGGAGACT CGGGACAACA AAACACATAC TCAGGAATTG CGCTGGCACA CAAGTCAGGA 
AATATAGAGC TGCAGTATTT CACTCCGTCT TTGACGTTGC CCAACCCTGC AGAGGAGGGA 
AAGTTGGAGA GCATAATGAC TGAAGCCTTT TGA