LOC103134385 cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103134385
Entrez Gene ID	103134385
Full Name	toll-like receptor 8
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007546615.2	XP_007546677.1	toll-like receptor 8

Pathways from BioSystems
Gene Ontology

The following LOC103134385 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103134385 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo131281	XM_007546615.2 Latest version!	Poecilia formosa toll-like receptor 8 (LOC103134385), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103134385 ( XM_007546615.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007546677.1 Poecilia formosa toll-like receptor 8 (LOC103134385), mRNA.

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CloneID	OPo131281
Clone ID Related Accession (Same CDS sequence)	XM_007546615.2
Accession Version	XM_007546615.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	toll-like receptor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006799955.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007546615.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGACTGCAA AGTGTTGGAT TTATCTGCTT CTCCTTGGTC TGTGCTGCCA TCTGGAGATC 
CAGCCTGCTG CCTGTAAACT TCATTGGACA GCGCAGTTTC TCTGTGATGT GACAACCTCC 
AATTCCTCTG AGGTCCAATT TGACTGCAGT GGCCGCGCCC TAGAATGGGT ACCTAGTGGG 
ATAACCACTA ATGTTACTGC TCTTGATTTA TCAAAAAATT ACCTGAAGAC CATTAAGGCT 
GATGCATTTT CCAAACTGCT GAATCTGACT TCTCTCAATC TCAACTTGGC AAACATGTAC 
AAGAAAGTGG TCTTCAAGGC AAGCCTTAGA AACCTAACAA AACTGCAGGA TTTGAAAATG 
AGTGCCAATG GCCTCGAAGA AATTCCAAGC TACCTACCTG CCAGTGTGGA AATCCTTGAG 
CTGAGCAGCA ACAACATTAC AAATGTGAAT CAAACGAACT TTGCCAACTT ACGAAACCTG 
ACACAACTGT GGTTATCCAA GAACTGTTAC TACTGGAATC CGTGTGGCAA ACCAGTGAAA 
ATCCAAAATA ACAGTTTTAC CCACATGACC AAATTGAAAA ATCTGGATTT GTCTTACAAC 
AACTTAAGTA ATGTTCCCAA AGGACTACCC CAGTCTTTAG AAGTGCTTAA TGTGAGTTGC 
AACAAAATAC AGTACATATT TAGGGAGGAT TTATCTGGCT TGAATAATTT AAAACTTCTT 
AAGATACAAG GGAACTGCCC CAGATGTGAA AATGCACCAT TTCCCTGTGT GCCTTGCCCT 
AATGGTTCCC TTGGAATTGA CCCTGATGCA TTTGAAAATC TGTCTGAACT TGAGGAAATC 
AATATGGGGG GAAACTCCTT AGAGTATCTG AATCCATCGT GGTTTAAGAA CCTGAGGAAG 
CTTAAACATC TTCTCCTTTC ATTTAACTTT TTGCTCAGAG AAGTTACTGG AAGGGCTGAA 
TTTCTGCGAT ACCTCCCAAG GCTTGAAGTG CTTGAATTTT CTTTCAACTA TGCTTTAAAA 
AGTTATCCTA ACACAATTAA TCTTTCAACT GAGTTTTCAA ATCTTAAGTC ATTAAGGACT 
TTGCATATGG AGGGTTTGGT CTTCCAGAAT ATTCAACCAG ATACGCTCAA GCCTCTCTAT 
GAAATTCGAA ATCTATCTGC TTTGAATTTG GGGACTAACT TTATTATTCA GGCTGATTCC 
ACCGTTTTCA ACAAATTTCA TCCATTGAAA ATGATTTATT TGGCAGAAAA CAGGCTGTAT 
CCCACTCCAC AGAGACAGCT TTCAAATTCA GTAGGGAGAT ATGTTCAAAG GTCAGACATC 
ACTGTTTCTT CCTATTTTAA ACTTCCTCCA AAAGACTTCA CCTTTGAATT ATCCCATGGC 
CTTATAAAGC AACAGTGCTT TAACTCTGGA CGGGTTCTTG TCCTCAGTTC AAATAACTTG 
TTCTTCATCT CACAAAAGCA ATTTGAAGGT TATGGAGATA TTGCGTGTCT GAATCTATCC 
AGCAATGGAT TCTCTGCTGC ACCAAATGGC ACAGAGCTAC TCTTCTCCTA CATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007546677.1
CDS	506..2065
Translation	MTAKCWIYLLLLGLCCHLEIQPAACKLHWTAQFLCDVTTSNSSEVQFDCSGRALEWVPSGITTNVTALDLSKNYLKTIKADAFSKLLNLTSLNLNLANMYKKVVFKASLRNLTKLQDLKMSANGLEEIPSYLPASVEILELSSNNITNVNQTNFANLRNLTQLWLSKNCYYWNPCGKPVKIQNNSFTHMTKLKNLDLSYNNLSNVPKGLPQSLEVLNVSCNKIQYIFREDLSGLNNLKLLKIQGNCPRCENAPFPCVPCPNGSLGIDPDAFENLSELEEINMGGNSLEYLNPSWFKNLRKLKHLLLSFNFLLREVTGRAEFLRYLPRLEVLEFSFNYALKSYPNTINLSTEFSNLKSLRTLHMEGLVFQNIQPDTLKPLYEIRNLSALNLGTNFIIQADSTVFNKFHPLKMIYLAENRLYPTPQRQLSNSVGRYVQRSDITVSSYFKLPPKDFTFELSHGLIKQQCFNSGRVLVLSSNNLFFISQKQFEGYGDIACLNLSSNGFSAAPNGTELLFSYIL

Target ORF information:

RefSeq Version	XM_007546615.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa toll-like receptor 8 (LOC103134385), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007546615.2

ORF Insert Sequence:

ATGACTGCAA AGTGTTGGAT TTATCTGCTT CTCCTTGGTC TGTGCTGCCA TCTGGAGATC 
CAGCCTGCTG CCTGTAAACT TCATTGGACA GCGCAGTTTC TCTGTGATGT GACAACCTCC 
AATTCCTCTG AGGTCCAATT TGACTGCAGT GGCCGCGCCC TAGAATGGGT ACCTAGTGGG 
ATAACCACTA ATGTTACTGC TCTTGATTTA TCAAAAAATT ACCTGAAGAC CATTAAGGCT 
GATGCATTTT CCAAACTGCT GAATCTGACT TCTCTCAATC TCAACTTGGC AAACATGTAC 
AAGAAAGTGG TCTTCAAGGC AAGCCTTAGA AACCTAACAA AACTGCAGGA TTTGAAAATG 
AGTGCCAATG GCCTCGAAGA AATTCCAAGC TACCTACCTG CCAGTGTGGA AATCCTTGAG 
CTGAGCAGCA ACAACATTAC AAATGTGAAT CAAACGAACT TTGCCAACTT ACGAAACCTG 
ACACAACTGT GGTTATCCAA GAACTGTTAC TACTGGAATC CGTGTGGCAA ACCAGTGAAA 
ATCCAAAATA ACAGTTTTAC CCACATGACC AAATTGAAAA ATCTGGATTT GTCTTACAAC 
AACTTAAGTA ATGTTCCCAA AGGACTACCC CAGTCTTTAG AAGTGCTTAA TGTGAGTTGC 
AACAAAATAC AGTACATATT TAGGGAGGAT TTATCTGGCT TGAATAATTT AAAACTTCTT 
AAGATACAAG GGAACTGCCC CAGATGTGAA AATGCACCAT TTCCCTGTGT GCCTTGCCCT 
AATGGTTCCC TTGGAATTGA CCCTGATGCA TTTGAAAATC TGTCTGAACT TGAGGAAATC 
AATATGGGGG GAAACTCCTT AGAGTATCTG AATCCATCGT GGTTTAAGAA CCTGAGGAAG 
CTTAAACATC TTCTCCTTTC ATTTAACTTT TTGCTCAGAG AAGTTACTGG AAGGGCTGAA 
TTTCTGCGAT ACCTCCCAAG GCTTGAAGTG CTTGAATTTT CTTTCAACTA TGCTTTAAAA 
AGTTATCCTA ACACAATTAA TCTTTCAACT GAGTTTTCAA ATCTTAAGTC ATTAAGGACT 
TTGCATATGG AGGGTTTGGT CTTCCAGAAT ATTCAACCAG ATACGCTCAA GCCTCTCTAT 
GAAATTCGAA ATCTATCTGC TTTGAATTTG GGGACTAACT TTATTATTCA GGCTGATTCC 
ACCGTTTTCA ACAAATTTCA TCCATTGAAA ATGATTTATT TGGCAGAAAA CAGGCTGTAT 
CCCACTCCAC AGAGACAGCT TTCAAATTCA GTAGGGAGAT ATGTTCAAAG GTCAGACATC 
ACTGTTTCTT CCTATTTTAA ACTTCCTCCA AAAGACTTCA CCTTTGAATT ATCCCATGGC 
CTTATAAAGC AACAGTGCTT TAACTCTGGA CGGGTTCTTG TCCTCAGTTC AAATAACTTG 
TTCTTCATCT CACAAAAGCA ATTTGAAGGT TATGGAGATA TTGCGTGTCT GAATCTATCC 
AGCAATGGAT TCTCTGCTGC ACCAAATGGC ACAGAGCTAC TCTTCTCCTA CATCCTGTGA