t cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	t
Entrez Gene ID	103134124
Full Name	T-box transcription factor T
Synonyms	t
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007546238.2	XP_007546300.1	brachyury protein

Pathways from BioSystems
Gene Ontology

The following t gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the t cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo130902	XM_007546238.2 Latest version!	Poecilia formosa T brachyury transcription factor (t), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

t ( XM_007546238.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007546300.1 Poecilia formosa T brachyury transcription factor (t), mRNA.

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CloneID	OPo130902
Clone ID Related Accession (Same CDS sequence)	XM_007546238.2
Accession Version	XM_007546238.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	brachyury protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006799954.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007546238.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACTG GCGCTGGTGA GTGTCCCAGC AAGGCCGCTC AGTACCGGGT GGACCACCTC 
CTCAGTGCGG TGGAGAGTGA GCTGCAGGCC GGCAGCGAGA AGGGAGACCC CACCGAGAGG 
GACCTGAAAG TGTCCCTGGA TGAACGGGAG CTGTGGCAAA AATTTAAGGA CCTCACAAAC 
GAGATGATAG TTACAAAGAA CGGCAGGCGC ATGTTCCCGG TTCTGAAAGT CAACGTCTCA 
GGACTGGACC CAAACGCCAT GTATTCCTTC TTGCTGGACT TTGTGTCGGC GGACAACCAC 
AGGTGGAAGT ACGTGAACGG GGAGTGGGTG CCGGGAGGTA AACCAGAGCC CCAGACCCCC 
AGCTGCGTGT ACATCCACCC AGACTCTCCA AACTTCGGGG CTCACTGGAT GAAGGCTCCG 
GTCTCCTTCA GCAAAGTCAA ACTCACCAAC AAGCTCAACG GCGGAGGTCA GATTATGTTA 
AATTCTTTGC ACAAATACGA GCCACGTATC CACATCGTTA GGGTCGGAGG CCCACGCAGG 
ATGATCACCA GCCACTCCTT CCTAGAGACG CAGTTCATCG CAGTAACAGC ATACCAAAAT 
GAAGAGATAA CAGCTCTAAA AATAAAGTAC AACCCTTTTG CCAAGGCCTT TCTAGATGCA 
AAGGAAAGGA ATGATCACAA AGACATGAGG GAAGAAGCTA ATGAGAATCC CCAGTCTGGT 
TACTCCCAAT TGGGTAGCTG GTTTATTCAC GGCACGGGAA CCCTTTGCCC CCCCACCAGT 
CCACACAGCC AGTTTGGGAG TCCCATCGCC CTGTCGCCCT CCCATGGCTG TGAGCGTTAC 
TCCAGTCTGA GGAGCCACCG CTCTGTGCCC TACAGCAGCC CGTATGCCCA TCGGACCAAC 
TCCCCCACTG GATATTCAGA TAACTCATCA TCCTGCTTGT CGATGCTCCC CAGCCATGAT 
AACTGGTCCA GTCTGCAGAT GCCGACCCAC ACCAGCATGA TGTCCATGAC CCACAGCTCC 
GCCTCTGCAG CCAACTCAAG TCAATACACC AGCCTGTGGT CTGTGAGTAA CTCCCCGCTG 
ACTCCAGTGT CCCAGAACGG GGGGTTAAGC AGCTGCCTCG GCTCCCAGTT CCTCCGAGGA 
TCGGCCAGTC ACTATCCCAG TCTGCCTCAC TCCGCCACAG CGCCCCCCTC TGCCTCTCCA 
ATGTATGATG GCAGCTCCGC CACAGAGGTG CAGGACGTTG TCCAGTATGA AGCTTCACCC 
CACGGCCGGC TGCCCTCAGC TTGGACCCCC GTCACGCCTC CATCCCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007546300.1
CDS	399..1709
Translation	MATGAGECPSKAAQYRVDHLLSAVESELQAGSEKGDPTERDLKVSLDERELWQKFKDLTNEMIVTKNGRRMFPVLKVNVSGLDPNAMYSFLLDFVSADNHRWKYVNGEWVPGGKPEPQTPSCVYIHPDSPNFGAHWMKAPVSFSKVKLTNKLNGGGQIMLNSLHKYEPRIHIVRVGGPRRMITSHSFLETQFIAVTAYQNEEITALKIKYNPFAKAFLDAKERNDHKDMREEANENPQSGYSQLGSWFIHGTGTLCPPTSPHSQFGSPIALSPSHGCERYSSLRSHRSVPYSSPYAHRTNSPTGYSDNSSSCLSMLPSHDNWSSLQMPTHTSMMSMTHSSASAANSSQYTSLWSVSNSPLTPVSQNGGLSSCLGSQFLRGSASHYPSLPHSATAPPSASPMYDGSSATEVQDVVQYEASPHGRLPSAWTPVTPPSL

Target ORF information:

RefSeq Version	XM_007546238.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa T brachyury transcription factor (t), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007546238.2

ORF Insert Sequence:

ATGGCGACTG GCGCTGGTGA GTGTCCCAGC AAGGCCGCTC AGTACCGGGT GGACCACCTC 
CTCAGTGCGG TGGAGAGTGA GCTGCAGGCC GGCAGCGAGA AGGGAGACCC CACCGAGAGG 
GACCTGAAAG TGTCCCTGGA TGAACGGGAG CTGTGGCAAA AATTTAAGGA CCTCACAAAC 
GAGATGATAG TTACAAAGAA CGGCAGGCGC ATGTTCCCGG TTCTGAAAGT CAACGTCTCA 
GGACTGGACC CAAACGCCAT GTATTCCTTC TTGCTGGACT TTGTGTCGGC GGACAACCAC 
AGGTGGAAGT ACGTGAACGG GGAGTGGGTG CCGGGAGGTA AACCAGAGCC CCAGACCCCC 
AGCTGCGTGT ACATCCACCC AGACTCTCCA AACTTCGGGG CTCACTGGAT GAAGGCTCCG 
GTCTCCTTCA GCAAAGTCAA ACTCACCAAC AAGCTCAACG GCGGAGGTCA GATTATGTTA 
AATTCTTTGC ACAAATACGA GCCACGTATC CACATCGTTA GGGTCGGAGG CCCACGCAGG 
ATGATCACCA GCCACTCCTT CCTAGAGACG CAGTTCATCG CAGTAACAGC ATACCAAAAT 
GAAGAGATAA CAGCTCTAAA AATAAAGTAC AACCCTTTTG CCAAGGCCTT TCTAGATGCA 
AAGGAAAGGA ATGATCACAA AGACATGAGG GAAGAAGCTA ATGAGAATCC CCAGTCTGGT 
TACTCCCAAT TGGGTAGCTG GTTTATTCAC GGCACGGGAA CCCTTTGCCC CCCCACCAGT 
CCACACAGCC AGTTTGGGAG TCCCATCGCC CTGTCGCCCT CCCATGGCTG TGAGCGTTAC 
TCCAGTCTGA GGAGCCACCG CTCTGTGCCC TACAGCAGCC CGTATGCCCA TCGGACCAAC 
TCCCCCACTG GATATTCAGA TAACTCATCA TCCTGCTTGT CGATGCTCCC CAGCCATGAT 
AACTGGTCCA GTCTGCAGAT GCCGACCCAC ACCAGCATGA TGTCCATGAC CCACAGCTCC 
GCCTCTGCAG CCAACTCAAG TCAATACACC AGCCTGTGGT CTGTGAGTAA CTCCCCGCTG 
ACTCCAGTGT CCCAGAACGG GGGGTTAAGC AGCTGCCTCG GCTCCCAGTT CCTCCGAGGA 
TCGGCCAGTC ACTATCCCAG TCTGCCTCAC TCCGCCACAG CGCCCCCCTC TGCCTCTCCA 
ATGTATGATG GCAGCTCCGC CACAGAGGTG CAGGACGTTG TCCAGTATGA AGCTTCACCC 
CACGGCCGGC TGCCCTCAGC TTGGACCCCC GTCACGCCTC CATCCCTTTG A