VFPPC_02183 cDNA ORF clone, Pochonia chlamydosporia 170

Gene
Clones
gRNAs

Summary

Gene Symbol	VFPPC_02183
Entrez Gene ID	28845871
Full Name	DNA helicase
Gene Type	protein-coding
Organism	Pochonia chlamydosporia 170

mRNA and Protein(s)

mRNA	Protein	Name
XM_018281877.1	XP_018138984.1	DNA helicase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Biosynthesis of Antibiotic Leucinostatins in Bio-control Fungus Purpureocillium lilacinum and Their Inhibition on Phytophthora Revealed by Genome Mining.
Wang G, Liu Z, Lin R, Li E, Mao Z, Ling J, Yang Y, Yin WB, Xie B
PLoS pathogens12(7)e1005685(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VFPPC_02183 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VFPPC_02183 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo214277	XM_018281877.1 Latest version!	Pochonia chlamydosporia 170 DNA helicase (VFPPC_02183), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VFPPC_02183 ( XM_018281877.1 ) cDNA ORF clone, Pochonia chlamydosporia 170 -> XP_018138984.1 Pochonia chlamydosporia 170 DNA helicase (VFPPC_02183), partial mRNA.

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CloneID	OPo214277
Clone ID Related Accession (Same CDS sequence)	XM_018281877.1
Accession Version	XM_018281877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-07
Organism	Pochonia chlamydosporia 170
Product	DNA helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_035790). COMPLETENESS: incomplete on both ends.

ATGTCTCAAT CAACGACTAA AAACGACGAG GCTGCCGCCA AGCCTACCAA GCCACAATCC 
TTCACAACCT TTTACAGGCC TTGTGCCGTT ATTCCTGGCC ACCTGTCAGG CCCGATCATC 
GGTGGAGCGT CTTTGGGAGT CTCAACAGAC ATTGACACCC GGATCATGTC ATCAAAGTCT 
CCCGGCAAAG ACCCATGGAT GGGATTCACC ATTGAGTTTC CACGCGGCAA GAACTGCGAG 
GACGAAGGCT TTGGCATGTG TCATTCACTG GATCCTTCAC AAGGGATGCC TGGGACCAGC 
CACTTTGGTG TTCCCGTACC AGCCGAAACG CTCAGAATCA ATGTCAAGTT TCCCCGCGGT 
GAAATAATGA GCACGTTTGA GAAAGCGGAC GACAAGAACC AGCTACGGAA AATGTTCCCC 
AATGTGCCCG CGGACAAAGA CCTGGTCCTC ATCGAGGTGT CCTGCAAAAG TGAGGTCGTG 
GTGGAGGGTC TAGGCTTGCC ATTCTCCAAC CCTGGTCATC CTGCTCACGG ATGGCTGAAT 
AATAATGAGC ATATTGCCAA GGACATGACA CTCATGAGCA TGTTCGAGCA AAGAGACTTC 
TGCATTGTGG TAGCAAAGAC ACCCTTGGCC ACACTGGAAA AGGAATGGGA CGAAGACAAA 
CTGCCCCCCC CTTTCCGCTA TCCTTACAAC GCAGATCAGT CATGGCAAGG CTTGTATCCG 
TACATCGACC AGCTCAAAGA AGCAAAAGGA CCACAGTTCA CGCCGGCCTG GACATATGTT 
GATGACAATT CTCACTTGGC TGTCACCTGC CAGTCTCAAG TCCAAGACGT CTTGTGGGTA 
GAAGAAGCCG CTCGTAATAT TCGTTCAACA CCACTGCCAG CCTATTTTGT CGACCTGCCC 
TCCAAAATGG GGTCAATGTA CTATGTGATT GTGCAACTGC CAAAGACTTT CATTCGCAAG 
TACGAGTCAG CTTGGCGTCG TTTGACCAAA TGCGACTTCT TTAAGCTCAA TTTGTACGGC 
AGCTCGGAGG AAGCGGAGTA TCTAATGCCG AAGGAGAAGT TCGATGACGA CCTCAAGAGG 
CCAAACTGGG ATGCAAAGTT TGTGAACAAC CACAATCACA GAGAAGAGCA TCTCGTGGAT 
CATCCTACAG AAGCAGAGGA TTTGGTGCTT CTGGTTCGCC GTCCTGCCAA ACACGAGCAT 
GGTCGTGACC CGCTGTACCA GAATTGGGTC GGACTACACT TCGACGCGGA CTTTAATGAG 
GCGAGACGCA AGGTTGAGGC CGCTTGGGAG TTGCGCCCGG GTGCTAAACC GTCCAACCCG 
GGCGTTTTTG GTCTTGCTGG AGGTTCCAAG GAGACTTTCG ACCCAGTCAA CACAGAGTCG 
CGCGAGTTAA AGGTCTTGTG GGATATGCGA GTTCACCGCG CTCTCCTTAG AGGGACGGGA 
TTTTACGAAT GCCTTCGAGC GTATGCCAGA AATCCTTTGG ACCCCTTGCA GGCAACCGCT 
GGCCATCAAT TTGAAGGGAC GCATTACAAA GAGCCTTTTA CTCGAGGCAC ATCACTGTCG 
CCGCCTGTGC AAAGTCTTTT GCCTACCTCT GATGAAGATT TTGTGACTGG CCTAATGGAA 
GAAGCTCATC CTGACGACCG GGTTCGGTTT CGAAACTATC TCAGCACGAG ACAGCTTGGG 
CTTGGTCTAA TCACTGCAGG CCCTGGATTC GGAAAGACAA CAGCCCTCGC CGTTGCGGCT 
CTTGGGATGA TTGCAAGCTT GGGACCAATT TACCTCACAG CACCGACCAA TGTCGCCGTC 
AATAATGCAG CAGTCCGCCT TGCTAATGTT GATGCCCGTG TAACGGAACG ACTCAACAAA 
GGCAAGATGA TAGATGATTC AACCTTTCAC CGTCGTCGCC TGATAATTCG TCCTTGGAAC 
GCTGCAGACG AGCTCATGGC TTTCGAAAAC CTACTCCGCG ACCCAGAAAG CCTGGAACAG 
GCCACACCGC GCCGAGGATG GAGGTCGAGC AAGTGGCAGC TGGAGTTATC AACCGCATTT 
TGGCTGCTCA TAGTCCTAAG GTCTCCGGCA GTCAGAGAGC TCAGCCCGGA TGACAGTCCT 
CTCTTGCATG AGCTGCGGAG GATTATTGAC GCTGATGCAG ACATGGCCAT TCTTCGGAGC 
AGAGCAACCG GCCAAGCCAG CTGGCAGGAG TACTTGGACT GGAAACGTGG CATTCCTACC 
AATGTGGTCA TCACAACGTT CTTCAACATC CTCCAAGGTG CCGCGGATGC TTTGTGTACC 
ACCCCTGCCA AGTCTTGCGA CGATTTCAAA GGGTGGAAGA CGAACAGGGC CAAGGGAATT 
GCTGTTGATG AGGCCGGCAA CATGACACGA CCAGATCTGT ACTCCGTCTG GGGCAATACT 
GGCATCCCCT GTGTCCTCGC TGGAGACGAC AAGCAGCTTC CACCATTTGT GGCAACCATG 
GAAGAAGTCG ATGAAGAGCA GAACGCCCTC AACAGACACG GCCCCGACGG CAAGATCTCG 
CCGCTGCTGT TTTTCAAGGC AATCGGCTGG CCAGTCTACA GACTCCGCAT CCAACATCGC 
ATGGCTCAGG GTCAATTCGA CTTTTGCCAC GACAAAGTGT ACAGCGACGT GCCGTTCACA 
TATGGGCCCG GTTGTCAGAT CCAGCTCCCA CAGCACGAAA TCGGCCGCAA GTTGGAAAGC 
TACCTCGGCG CCAAGTATCC CGAGCTGAGG CCCGCCGCAC CCGGTCAACT CGCCCCGGTC 
TTTGTGCACT GTCAAGGCTC ATACACCTTC GTCGATCCCG TGACGAAGTC GAAGCTGAAC 
ATTGACCAGG TCAAGATCGG CCTGGACTTG ATCTTGGATT TCGTCAGGGA AGCCAGTGTC 
GATCCCAAAG ACATTGCCAT TATCAGTCCA TATGCCGCCA ACGTGGACAT GATTGCCAAG 
ATACGGAGGA ACCACAAGTA CACTGCTCTT GCCAACATGC CCCCGGCTTC AACCGTGGAC 
TCTTTCCAGG GCCAAGAGGG AGACATCACC GTCGTTTTAA TGGGCACCAC TCGGGCAGTA 
GGACCTGGCT TCACTTGCAA CGAGAACCGG CTCAATGTCA TGCTCACTCG CCACAAGAGT 
GGCCTAGTCA TAATTGGAGA CCTGGATGTG ACTGGAAATG TCGAAACTCT CCTGAAAAAG 
GCAAAAGAGA GGGCCAAGAA GGGCAAGGGC AAGGGCAAGG GCAAGTCCAT GGCAAACAAT 
CCTGATGTCA TGCAGGTGGA ATCCAACACC GGAGAACGGG TGTATGTCAA GGTCCACATG 
TTGCGTGACC TCTGCGTTTC TTCGCACGAA TCGGGCCGTG TCATCCACAT CCAAGTCCCC 
GAAAAGGACA AAAGTGGCAA AGACGGAGCG AATGCTGGGA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018138984.1
CDS	1..3405
Translation	MSQSTTKNDEAAAKPTKPQSFTTFYRPCAVIPGHLSGPIIGGASLGVSTDIDTRIMSSKSPGKDPWMGFTIEFPRGKNCEDEGFGMCHSLDPSQGMPGTSHFGVPVPAETLRINVKFPRGEIMSTFEKADDKNQLRKMFPNVPADKDLVLIEVSCKSEVVVEGLGLPFSNPGHPAHGWLNNNEHIAKDMTLMSMFEQRDFCIVVAKTPLATLEKEWDEDKLPPPFRYPYNADQSWQGLYPYIDQLKEAKGPQFTPAWTYVDDNSHLAVTCQSQVQDVLWVEEAARNIRSTPLPAYFVDLPSKMGSMYYVIVQLPKTFIRKYESAWRRLTKCDFFKLNLYGSSEEAEYLMPKEKFDDDLKRPNWDAKFVNNHNHREEHLVDHPTEAEDLVLLVRRPAKHEHGRDPLYQNWVGLHFDADFNEARRKVEAAWELRPGAKPSNPGVFGLAGGSKETFDPVNTESRELKVLWDMRVHRALLRGTGFYECLRAYARNPLDPLQATAGHQFEGTHYKEPFTRGTSLSPPVQSLLPTSDEDFVTGLMEEAHPDDRVRFRNYLSTRQLGLGLITAGPGFGKTTALAVAALGMIASLGPIYLTAPTNVAVNNAAVRLANVDARVTERLNKGKMIDDSTFHRRRLIIRPWNAADELMAFENLLRDPESLEQATPRRGWRSSKWQLELSTAFWLLIVLRSPAVRELSPDDSPLLHELRRIIDADADMAILRSRATGQASWQEYLDWKRGIPTNVVITTFFNILQGAADALCTTPAKSCDDFKGWKTNRAKGIAVDEAGNMTRPDLYSVWGNTGIPCVLAGDDKQLPPFVATMEEVDEEQNALNRHGPDGKISPLLFFKAIGWPVYRLRIQHRMAQGQFDFCHDKVYSDVPFTYGPGCQIQLPQHEIGRKLESYLGAKYPELRPAAPGQLAPVFVHCQGSYTFVDPVTKSKLNIDQVKIGLDLILDFVREASVDPKDIAIISPYAANVDMIAKIRRNHKYTALANMPPASTVDSFQGQEGDITVVLMGTTRAVGPGFTCNENRLNVMLTRHKSGLVIIGDLDVTGNVETLLKKAKERAKKGKGKGKGKSMANNPDVMQVESNTGERVYVKVHMLRDLCVSSHESGRVIHIQVPEKDKSGKDGANAGR

Target ORF information:

RefSeq Version	XM_018281877.1
Organism	Pochonia chlamydosporia 170
Definition	Pochonia chlamydosporia 170 DNA helicase (VFPPC_02183), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018281877.1

ORF Insert Sequence:

ATGTCTCAAT CAACGACTAA AAACGACGAG GCTGCCGCCA AGCCTACCAA GCCACAATCC 
TTCACAACCT TTTACAGGCC TTGTGCCGTT ATTCCTGGCC ACCTGTCAGG CCCGATCATC 
GGTGGAGCGT CTTTGGGAGT CTCAACAGAC ATTGACACCC GGATCATGTC ATCAAAGTCT 
CCCGGCAAAG ACCCATGGAT GGGATTCACC ATTGAGTTTC CACGCGGCAA GAACTGCGAG 
GACGAAGGCT TTGGCATGTG TCATTCACTG GATCCTTCAC AAGGGATGCC TGGGACCAGC 
CACTTTGGTG TTCCCGTACC AGCCGAAACG CTCAGAATCA ATGTCAAGTT TCCCCGCGGT 
GAAATAATGA GCACGTTTGA GAAAGCGGAC GACAAGAACC AGCTACGGAA AATGTTCCCC 
AATGTGCCCG CGGACAAAGA CCTGGTCCTC ATCGAGGTGT CCTGCAAAAG TGAGGTCGTG 
GTGGAGGGTC TAGGCTTGCC ATTCTCCAAC CCTGGTCATC CTGCTCACGG ATGGCTGAAT 
AATAATGAGC ATATTGCCAA GGACATGACA CTCATGAGCA TGTTCGAGCA AAGAGACTTC 
TGCATTGTGG TAGCAAAGAC ACCCTTGGCC ACACTGGAAA AGGAATGGGA CGAAGACAAA 
CTGCCCCCCC CTTTCCGCTA TCCTTACAAC GCAGATCAGT CATGGCAAGG CTTGTATCCG 
TACATCGACC AGCTCAAAGA AGCAAAAGGA CCACAGTTCA CGCCGGCCTG GACATATGTT 
GATGACAATT CTCACTTGGC TGTCACCTGC CAGTCTCAAG TCCAAGACGT CTTGTGGGTA 
GAAGAAGCCG CTCGTAATAT TCGTTCAACA CCACTGCCAG CCTATTTTGT CGACCTGCCC 
TCCAAAATGG GGTCAATGTA CTATGTGATT GTGCAACTGC CAAAGACTTT CATTCGCAAG 
TACGAGTCAG CTTGGCGTCG TTTGACCAAA TGCGACTTCT TTAAGCTCAA TTTGTACGGC 
AGCTCGGAGG AAGCGGAGTA TCTAATGCCG AAGGAGAAGT TCGATGACGA CCTCAAGAGG 
CCAAACTGGG ATGCAAAGTT TGTGAACAAC CACAATCACA GAGAAGAGCA TCTCGTGGAT 
CATCCTACAG AAGCAGAGGA TTTGGTGCTT CTGGTTCGCC GTCCTGCCAA ACACGAGCAT 
GGTCGTGACC CGCTGTACCA GAATTGGGTC GGACTACACT TCGACGCGGA CTTTAATGAG 
GCGAGACGCA AGGTTGAGGC CGCTTGGGAG TTGCGCCCGG GTGCTAAACC GTCCAACCCG 
GGCGTTTTTG GTCTTGCTGG AGGTTCCAAG GAGACTTTCG ACCCAGTCAA CACAGAGTCG 
CGCGAGTTAA AGGTCTTGTG GGATATGCGA GTTCACCGCG CTCTCCTTAG AGGGACGGGA 
TTTTACGAAT GCCTTCGAGC GTATGCCAGA AATCCTTTGG ACCCCTTGCA GGCAACCGCT 
GGCCATCAAT TTGAAGGGAC GCATTACAAA GAGCCTTTTA CTCGAGGCAC ATCACTGTCG 
CCGCCTGTGC AAAGTCTTTT GCCTACCTCT GATGAAGATT TTGTGACTGG CCTAATGGAA 
GAAGCTCATC CTGACGACCG GGTTCGGTTT CGAAACTATC TCAGCACGAG ACAGCTTGGG 
CTTGGTCTAA TCACTGCAGG CCCTGGATTC GGAAAGACAA CAGCCCTCGC CGTTGCGGCT 
CTTGGGATGA TTGCAAGCTT GGGACCAATT TACCTCACAG CACCGACCAA TGTCGCCGTC 
AATAATGCAG CAGTCCGCCT TGCTAATGTT GATGCCCGTG TAACGGAACG ACTCAACAAA 
GGCAAGATGA TAGATGATTC AACCTTTCAC CGTCGTCGCC TGATAATTCG TCCTTGGAAC 
GCTGCAGACG AGCTCATGGC TTTCGAAAAC CTACTCCGCG ACCCAGAAAG CCTGGAACAG 
GCCACACCGC GCCGAGGATG GAGGTCGAGC AAGTGGCAGC TGGAGTTATC AACCGCATTT 
TGGCTGCTCA TAGTCCTAAG GTCTCCGGCA GTCAGAGAGC TCAGCCCGGA TGACAGTCCT 
CTCTTGCATG AGCTGCGGAG GATTATTGAC GCTGATGCAG ACATGGCCAT TCTTCGGAGC 
AGAGCAACCG GCCAAGCCAG CTGGCAGGAG TACTTGGACT GGAAACGTGG CATTCCTACC 
AATGTGGTCA TCACAACGTT CTTCAACATC CTCCAAGGTG CCGCGGATGC TTTGTGTACC 
ACCCCTGCCA AGTCTTGCGA CGATTTCAAA GGGTGGAAGA CGAACAGGGC CAAGGGAATT 
GCTGTTGATG AGGCCGGCAA CATGACACGA CCAGATCTGT ACTCCGTCTG GGGCAATACT 
GGCATCCCCT GTGTCCTCGC TGGAGACGAC AAGCAGCTTC CACCATTTGT GGCAACCATG 
GAAGAAGTCG ATGAAGAGCA GAACGCCCTC AACAGACACG GCCCCGACGG CAAGATCTCG 
CCGCTGCTGT TTTTCAAGGC AATCGGCTGG CCAGTCTACA GACTCCGCAT CCAACATCGC 
ATGGCTCAGG GTCAATTCGA CTTTTGCCAC GACAAAGTGT ACAGCGACGT GCCGTTCACA 
TATGGGCCCG GTTGTCAGAT CCAGCTCCCA CAGCACGAAA TCGGCCGCAA GTTGGAAAGC 
TACCTCGGCG CCAAGTATCC CGAGCTGAGG CCCGCCGCAC CCGGTCAACT CGCCCCGGTC 
TTTGTGCACT GTCAAGGCTC ATACACCTTC GTCGATCCCG TGACGAAGTC GAAGCTGAAC 
ATTGACCAGG TCAAGATCGG CCTGGACTTG ATCTTGGATT TCGTCAGGGA AGCCAGTGTC 
GATCCCAAAG ACATTGCCAT TATCAGTCCA TATGCCGCCA ACGTGGACAT GATTGCCAAG 
ATACGGAGGA ACCACAAGTA CACTGCTCTT GCCAACATGC CCCCGGCTTC AACCGTGGAC 
TCTTTCCAGG GCCAAGAGGG AGACATCACC GTCGTTTTAA TGGGCACCAC TCGGGCAGTA 
GGACCTGGCT TCACTTGCAA CGAGAACCGG CTCAATGTCA TGCTCACTCG CCACAAGAGT 
GGCCTAGTCA TAATTGGAGA CCTGGATGTG ACTGGAAATG TCGAAACTCT CCTGAAAAAG 
GCAAAAGAGA GGGCCAAGAA GGGCAAGGGC AAGGGCAAGG GCAAGTCCAT GGCAAACAAT 
CCTGATGTCA TGCAGGTGGA ATCCAACACC GGAGAACGGG TGTATGTCAA GGTCCACATG 
TTGCGTGACC TCTGCGTTTC TTCGCACGAA TCGGGCCGTG TCATCCACAT CCAAGTCCCC 
GAAAAGGACA AAAGTGGCAA AGACGGAGCG AATGCTGGGA GGTGA