UPF1 cDNA ORF clone, Phascolarctos cinereus(koala)

Gene
Clones
gRNAs

Summary

Gene Symbol	UPF1
Entrez Gene ID	110223386
Full Name	UPF1, RNA helicase and ATPase
Gene Type	protein-coding
Organism	Phascolarctos cinereus(koala)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021008876.1	XP_020864535.1	LOW QUALITY PROTEIN: regulator of nonsense transcripts 1

Pathways from BioSystems
Gene Ontology

The following UPF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UPF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPh271140	XM_021008876.1 Latest version!	Phascolarctos cinereus UPF1, RNA helicase and ATPase (UPF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UPF1 ( XM_021008876.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020864535.1 Phascolarctos cinereus UPF1, RNA helicase and ATPase (UPF1), mRNA.

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CloneID	OPh271140
Clone ID Related Accession (Same CDS sequence)	XM_021008876.1
Accession Version	XM_021008876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	LOW QUALITY PROTEIN: regulator of nonsense transcripts 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACTCTCA CCTTCCTGGA CACTGAGGAG 
GCCGAGCTGC TGGGCGCAGA CACCCAGGGC TCCGAGTTCG AGTTCACCGA CTTCACCCTC 
CCCAGCCAGA CCCAGACGCC CCCCGGGGGG CCTGGCGGCG GCGGAGGCGG CGCGGGCGGG 
CCCGGAGCCG CGGGCGTCGG GGGAGCCGCG GGCCAGCTCG ACGCGCAGGT TAATGGACCT 
GATGGTATCC TGCAGAACGG TGCTGTGGAT GACAGCGTAG CTAAAACTAG CCAACTGTTG 
GCCGAATTGA ATTTTGAAGA AGATGAAGAA GATACTTACT ATACAAAGGA TCTCCCTGTG 
CATGCTTGCA GTTACTGTGG CATCCATGAC CCGGCTTGTG TGGTTTACTG CAATACTAGC 
AAGAAATGGT TTTGTAACGG ACGTGGAAAC ACTTCTGGCA GCCACATTGT AAATCACCTA 
GTAAGAGCAA AATGCAAAGA GGTCACCCTC CACAAGGATG GGCCCCTGGG AGAGACAGTG 
CTGGAGTGTT ACAATTGTGG CTGTCGAAAT GTGTTTCTCC TGGGCTTCAT ACCCGCCAAA 
GCTGATTCCG TGGTGGTGCT CCTCTGCAGG CAACCCTGTG CCAGTCAGAG CAGCTTAAAG 
GATATCAATT GGGACAGCTC CCAGTGGCAG CCTCTGATCC AAGATCGCTG CTTTCTTTCC 
TGGCTGGTAA AGATTCCTTC TGAGCAGGAG CAGCTGAGAG CACGGCAGAT TACAGCTCAA 
CAGATCAACA AACTCGAGGA ACTTTGGAAG GAAAATCCCT CAGCAACTCT TGAAGATCTG 
GAAAAGCCAG GTGTTGATGA GGAACCACAG CATGTCTTGC TTCGCTATGA GGATGCTTAC 
CAGTATCAAA ATATATTCGG CCCACTTGTC AAACTGGAAG CAGATTATGA TAAGAAACTT 
AAAGAGTCCC AGACCCAAGA TAACATCACA GTCAGATGGG ACCTGGGCCT CAACAAGAAG 
AGAATCGCTT ACTTCACGTT GCCCAAGACT GACTCTGATA TGCGTCTTAT GCAGGGAGAT 
GAAATCTGCC TTCGTTATAA AGGGGACCTC GCCCCGCTGT GGAAGGGAAT CGGCCATGTT 
ATTAAAGTCC CAGATAATTA TGGAGATGAA ATTGCGATTG AATTGCGGAG TAGTGTCGGA 
GCCCCTGTAG AAGTTACTCA TAACTTCCAA GTGGATTTTG TATGGAAGTC CACTTCATTT 
GACAGAATGC AGAGTGCATT AAAAACATTT GCTGTGGATG AAACCTCAGT ATCTGGCTAC 
ATTTACCACA AACTGTTGGG TCATGAAGTG GAGGATGTGA TTATTAAATG CCAGCTGCCA 
AAACGTTTTA CAGCACAAGG ACTCCCTGAC CTCAATCATT CTCAGGTATA TGCAGTAAAG 
ACTGTTTTGC AGCGACCTTT GAGCCTTATC CAGGGACCAC CTGGTACAGG GAAGACTGTA 
ACATCAGCTA CAATTGTCTA CCATTTGGCT AGGCAAGGCA ATGGGCCTGT GCTAGTTTGT 
GCACCAAGCA ACATAGCTGT GGATCAGTTG ACTGAGAAGA TTCATCAGAC TGGACTGAAA 
GTTGTTCGTC TTTGCGCTAA AAGCCGGGAA GCAATTGATT CTCCCGTATC TTTTTTGGCA 
CTGCACAACC AGATCAGGAA TATGGATAGT ATGCCTGAAC TCCAGAAATT ACAGCAACTG 
AAAGATGAAA CAGGCGAACT TTCATCCGCT GATGAAAAGC GTTACAGAGC ATTGAAACGC 
ACAGCTGAGC GAGAATTGCT CATGAATGCA GATGTGATCT GCTGCACATG CGTGGGAGCA 
GGTGATCCGA GGCTCGCCAA GATGCAATTC CGATCCATTT TAATTGACGA AAGCACACAG 
GCTACTGAAC CAGAGTGTAT GGTGCCAGTT GTTCTTGGAG CCAAACAGTT GATCCTTGTA 
GGAGATCATT GCCAGCTGGG TCCTGTAGTC ATGTGCAAGA AAGCTGCAAA AGCAGGCTTG 
TCTCAGTCGT TGTTTGAGCG GCTAGTGGTG TTGGGGATCC GTCCAATCCG CCTGCAAGTC 
CAGTATCGAA TGCATCCTGC CCTTAGTGCT TTCCCATCAA ACATCTTTTA TGAGGGTTCC 
CTTCAGAATG GTGTCACTGC AGCTGATCGT GTGAAAAAGG GATTTGATTT CCAGTGGCCC 
CAGCCAGATA AACCAATGTT CTTTTATGTG ACCCAGGGCC AAGAAGAAAT TGCCAGTTCA 
GGCACCTCTT ATTTGAACAG AACGGAAGCA GCAAATGTGG AAAAGATAAC TACAAAGCTC 
CTGAAAGCTG GTGCCAAGCC AGATCAGATT GGTATTATTA CACCTTATGA GGGTCAACGA 
TCTTATCTGG TGCAATACAT GCAATTTAGT GGCTCCCTGC ATACAAAACT CTACCAGGAA 
GTGGAAATAG CTAGTGTTGA TGCATTCCAG GGAAGAGAAA AGGACTTCAT CATCCTTTCC 
TGTGTGAGAG CCAATGAGCA CCAAGGAATT GGATTTTTAA ATGACCCCAG GCGTCTGAAT 
GTGGCTCTTA CCCGAGCAAG GTATGGAGTA ATTATTGTCG GCAACCCCAA AGCTCTCTCC 
AAGCAACCAC TTTGGAACCA TCTATTGAAC TACTACAAGG AACAGAAAGT ACTGGTAGAA 
GGACCCCTAA ACAACCTTCG GGAGAGCCTT ATGCAATTCA GCAAGCCCCG AAAACTTGTC 
AATACCATCA ACCCAGGAGC CCGGTTCATG ACTACTGCCA TGTATGACGC ACGTGAAGCT 
ATTATCCCAG GATCTGTCTA TGATCGGAGC AGTCAAGGAC GTCCATCAAA CATGTATTTT 
CAAACTCATG ATCAGATTGG GATGATTAGC ACTGGACCTA GTCATGTCAC TGCTATGAAC 
ATTCCTATTC CTTTCAACCT CGTGATGCCA CCAATGCCAC CACCAGGCTA TTTCGGCCAA 
GCCAATGGAC CAGCTGCAGG CCGTGGAACA CCAAAGGGGA AGACTGGTCG TGGGGGACGC 
CAAAAAAATC GCTTTGGCAT TCCTGGAACT AGCCAGTCAA ATCTTCCAAA TAGTCAGGCC 
AGTCAAGATG TGGTATCTCA GCCCTTCTCC CAGGGTCCAT TGACTCAGGG CTATATTTCC 
ATGAGTCAGC CATCACAGAT GAGCCAGCCA GGGCTCTCCC AACCAGAATT ATCCCAGGAC 
AGTTACCTTG GTGATGAGTT TAAATCGCAG ATTGATGTGG CTCTGTCACA AGACTCTACA 
TACCAAGGAG AACGGGCATA CCAACACGGT GGAGTGACAG GGCTGTCACA GTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020864535.1
CDS	446..3802
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGGGGGAGGPGAAGVGGAAGQLDAQVNGPDGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISTGPSHVTAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGIPGTSQSNLPNSQASQDVVSQPFSQGPLTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_021008876.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus UPF1, RNA helicase and ATPase (UPF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021008876.1

ORF Insert Sequence:

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACTCTCA CCTTCCTGGA CACTGAGGAG 
GCCGAGCTGC TGGGCGCAGA CACCCAGGGC TCCGAGTTCG AGTTCACCGA CTTCACCCTC 
CCCAGCCAGA CCCAGACGCC CCCCGGGGGG CCTGGCGGCG GCGGAGGCGG CGCGGGCGGG 
CCCGGAGCCG CGGGCGTCGG GGGAGCCGCG GGCCAGCTCG ACGCGCAGGT TAATGGACCT 
GATGGTATCC TGCAGAACGG TGCTGTGGAT GACAGCGTAG CTAAAACTAG CCAACTGTTG 
GCCGAATTGA ATTTTGAAGA AGATGAAGAA GATACTTACT ATACAAAGGA TCTCCCTGTG 
CATGCTTGCA GTTACTGTGG CATCCATGAC CCGGCTTGTG TGGTTTACTG CAATACTAGC 
AAGAAATGGT TTTGTAACGG ACGTGGAAAC ACTTCTGGCA GCCACATTGT AAATCACCTA 
GTAAGAGCAA AATGCAAAGA GGTCACCCTC CACAAGGATG GGCCCCTGGG AGAGACAGTG 
CTGGAGTGTT ACAATTGTGG CTGTCGAAAT GTGTTTCTCC TGGGCTTCAT ACCCGCCAAA 
GCTGATTCCG TGGTGGTGCT CCTCTGCAGG CAACCCTGTG CCAGTCAGAG CAGCTTAAAG 
GATATCAATT GGGACAGCTC CCAGTGGCAG CCTCTGATCC AAGATCGCTG CTTTCTTTCC 
TGGCTGGTAA AGATTCCTTC TGAGCAGGAG CAGCTGAGAG CACGGCAGAT TACAGCTCAA 
CAGATCAACA AACTCGAGGA ACTTTGGAAG GAAAATCCCT CAGCAACTCT TGAAGATCTG 
GAAAAGCCAG GTGTTGATGA GGAACCACAG CATGTCTTGC TTCGCTATGA GGATGCTTAC 
CAGTATCAAA ATATATTCGG CCCACTTGTC AAACTGGAAG CAGATTATGA TAAGAAACTT 
AAAGAGTCCC AGACCCAAGA TAACATCACA GTCAGATGGG ACCTGGGCCT CAACAAGAAG 
AGAATCGCTT ACTTCACGTT GCCCAAGACT GACTCTGATA TGCGTCTTAT GCAGGGAGAT 
GAAATCTGCC TTCGTTATAA AGGGGACCTC GCCCCGCTGT GGAAGGGAAT CGGCCATGTT 
ATTAAAGTCC CAGATAATTA TGGAGATGAA ATTGCGATTG AATTGCGGAG TAGTGTCGGA 
GCCCCTGTAG AAGTTACTCA TAACTTCCAA GTGGATTTTG TATGGAAGTC CACTTCATTT 
GACAGAATGC AGAGTGCATT AAAAACATTT GCTGTGGATG AAACCTCAGT ATCTGGCTAC 
ATTTACCACA AACTGTTGGG TCATGAAGTG GAGGATGTGA TTATTAAATG CCAGCTGCCA 
AAACGTTTTA CAGCACAAGG ACTCCCTGAC CTCAATCATT CTCAGGTATA TGCAGTAAAG 
ACTGTTTTGC AGCGACCTTT GAGCCTTATC CAGGGACCAC CTGGTACAGG GAAGACTGTA 
ACATCAGCTA CAATTGTCTA CCATTTGGCT AGGCAAGGCA ATGGGCCTGT GCTAGTTTGT 
GCACCAAGCA ACATAGCTGT GGATCAGTTG ACTGAGAAGA TTCATCAGAC TGGACTGAAA 
GTTGTTCGTC TTTGCGCTAA AAGCCGGGAA GCAATTGATT CTCCCGTATC TTTTTTGGCA 
CTGCACAACC AGATCAGGAA TATGGATAGT ATGCCTGAAC TCCAGAAATT ACAGCAACTG 
AAAGATGAAA CAGGCGAACT TTCATCCGCT GATGAAAAGC GTTACAGAGC ATTGAAACGC 
ACAGCTGAGC GAGAATTGCT CATGAATGCA GATGTGATCT GCTGCACATG CGTGGGAGCA 
GGTGATCCGA GGCTCGCCAA GATGCAATTC CGATCCATTT TAATTGACGA AAGCACACAG 
GCTACTGAAC CAGAGTGTAT GGTGCCAGTT GTTCTTGGAG CCAAACAGTT GATCCTTGTA 
GGAGATCATT GCCAGCTGGG TCCTGTAGTC ATGTGCAAGA AAGCTGCAAA AGCAGGCTTG 
TCTCAGTCGT TGTTTGAGCG GCTAGTGGTG TTGGGGATCC GTCCAATCCG CCTGCAAGTC 
CAGTATCGAA TGCATCCTGC CCTTAGTGCT TTCCCATCAA ACATCTTTTA TGAGGGTTCC 
CTTCAGAATG GTGTCACTGC AGCTGATCGT GTGAAAAAGG GATTTGATTT CCAGTGGCCC 
CAGCCAGATA AACCAATGTT CTTTTATGTG ACCCAGGGCC AAGAAGAAAT TGCCAGTTCA 
GGCACCTCTT ATTTGAACAG AACGGAAGCA GCAAATGTGG AAAAGATAAC TACAAAGCTC 
CTGAAAGCTG GTGCCAAGCC AGATCAGATT GGTATTATTA CACCTTATGA GGGTCAACGA 
TCTTATCTGG TGCAATACAT GCAATTTAGT GGCTCCCTGC ATACAAAACT CTACCAGGAA 
GTGGAAATAG CTAGTGTTGA TGCATTCCAG GGAAGAGAAA AGGACTTCAT CATCCTTTCC 
TGTGTGAGAG CCAATGAGCA CCAAGGAATT GGATTTTTAA ATGACCCCAG GCGTCTGAAT 
GTGGCTCTTA CCCGAGCAAG GTATGGAGTA ATTATTGTCG GCAACCCCAA AGCTCTCTCC 
AAGCAACCAC TTTGGAACCA TCTATTGAAC TACTACAAGG AACAGAAAGT ACTGGTAGAA 
GGACCCCTAA ACAACCTTCG GGAGAGCCTT ATGCAATTCA GCAAGCCCCG AAAACTTGTC 
AATACCATCA ACCCAGGAGC CCGGTTCATG ACTACTGCCA TGTATGACGC ACGTGAAGCT 
ATTATCCCAG GATCTGTCTA TGATCGGAGC AGTCAAGGAC GTCCATCAAA CATGTATTTT 
CAAACTCATG ATCAGATTGG GATGATTAGC ACTGGACCTA GTCATGTCAC TGCTATGAAC 
ATTCCTATTC CTTTCAACCT CGTGATGCCA CCAATGCCAC CACCAGGCTA TTTCGGCCAA 
GCCAATGGAC CAGCTGCAGG CCGTGGAACA CCAAAGGGGA AGACTGGTCG TGGGGGACGC 
CAAAAAAATC GCTTTGGCAT TCCTGGAACT AGCCAGTCAA ATCTTCCAAA TAGTCAGGCC 
AGTCAAGATG TGGTATCTCA GCCCTTCTCC CAGGGTCCAT TGACTCAGGG CTATATTTCC 
ATGAGTCAGC CATCACAGAT GAGCCAGCCA GGGCTCTCCC AACCAGAATT ATCCCAGGAC 
AGTTACCTTG GTGATGAGTT TAAATCGCAG ATTGATGTGG CTCTGTCACA AGACTCTACA 
TACCAAGGAG AACGGGCATA CCAACACGGT GGAGTGACAG GGCTGTCACA GTATTAG