BRAF cDNA ORF clone, Phascolarctos cinereus(koala)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAF
Entrez Gene ID	110216339
Full Name	B-Raf proto-oncogene, serine/threonine kinase
Gene Type	protein-coding
Organism	Phascolarctos cinereus(koala)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020998143.1	XP_020853802.1	serine/threonine-protein kinase B-raf isoform X1
XM_020998144.1	XP_020853803.1	serine/threonine-protein kinase B-raf isoform X2
XM_020998145.1	XP_020853804.1	serine/threonine-protein kinase B-raf isoform X3
XM_020998148.1	XP_020853807.1	serine/threonine-protein kinase B-raf isoform X6
XM_020998146.1	XP_020853805.1	serine/threonine-protein kinase B-raf isoform X4
XM_020998147.1	XP_020853806.1	serine/threonine-protein kinase B-raf isoform X5
XM_020998149.1	XP_020853808.1	serine/threonine-protein kinase B-raf isoform X7

Pathways from BioSystems
Gene Ontology

The following BRAF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPh263397	XM_020998143.1 Latest version!	Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPh263398	XM_020998144.1 Latest version!	Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPh263399	XM_020998145.1 Latest version!	Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPh263400	XM_020998146.1 Latest version!	Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPh263401	XM_020998147.1 Latest version!	Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPh263402	XM_020998148.1 Latest version!	Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPh263403	XM_020998149.1 Latest version!	Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAF ( XM_020998143.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020853802.1 Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPh263397
Clone ID Related Accession (Same CDS sequence)	XM_020998143.1
Accession Version	XM_020998143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	serine/threonine-protein kinase B-raf isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344022.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC TGAGCGGCGG CGGCGGCGGC GGCGCGGAGC AGGGCCCCGC TCTCTTCAAC 
GGGGACATGG ATCCCGAGGC TGGCGCCGGC TCGGCGTCCT CCGCCGAGCC GGCCATCCCC 
GAGGAGGTGT GGAACATCAA GCAAATGATT AAATTGACAC AGGAACACAT AGAGGCCCTA 
TTGGACAAGT TCGGAGGGGA ACATAATCCA CCATCAATCT ACCTGGAGGC CTATGAAGAA 
TATACCAGCA AGCTAGATGC ACTTCAACAA CGGGAACAAC AATTATTAGA GTCCATGGGG 
AATGGAACTG ATTTTTCTGT TTCTAGCTCA GCTTCAACAG ACACAGTTAC ATCTTCTTCC 
TCTTCTAGCC TTTCAGTGGC ACCTTCATCT CTTGCAGTTT TTCAGAATCC TACAGATGTA 
TCAAGAAGCA ACCCTAAGTC ACCACAAAAA CCAATTGTTA GAGTCTTCCT GCCCAACAAA 
CAGAGGACTG TGGTGCCAGC AAGATGTGGG GTGACAGTCC GAGACAGTCT AAAGAAAGCA 
TTGATGATGA GAGGCCTGAT CCCAGAATGC TGTGCTGTGT ACAGAAGACA GGATGGAGAG 
AAGAAGCCTA TTGGCTGGGA CACTGATATT TCCTGGCTTA CTGGAGAAGA ATTGCACGTA 
GAAGTTTTGG AGAATGTTCC CCTAACGACA CACAATTTTG TACGGAAGAC ATTTTTTACC 
TTAGCATTCT GTGACTTTTG TCGAAAGTTG CTTTTCCAAG GTTTCCGCTG TCAGACATGT 
GGTTACAAAT TTCACCAACG CTGTAGTACA GAGGTGCCAC TGATGTGTGT TAATTATGAC 
CAGCTTGATT TGCTGTTTGT CTCCAAGTTC TTTGAACACC ACCCAATACC GCAGGAGGAG 
GCCTCCTTAG GAGAGAGCAC CCTTACGTCG GGATCATCCC CTTCAGCACC CCCCTCAGAT 
TCTATTGGGC CCCCAATTCT TACCAGTCCA TCGCCTTCAA AATCCATTCC AATCCCACAG 
CCTTTTCGAC CAGCAGATGA GGATCATCGG AACCAGTTTG GGCAACGAGA CCGGTCCTCA 
TCGGCTCCCA ATGTGCATAT AAATACAATA GAACCTGTCA ATATTGATGA CTTGATTAGA 
GACCAAGGGT TACGGGGTGA TGGAGCCCCC TTGAACCAAC TGATGCGCTG TCTTCGGAAA 
TACCAATCCC GGACTCCCAG TCCCCTCCTA CATTCTGTCC CCAGTGAAAT AGTGTTTGAT 
TTTGAGCCTG GCCCAGTGTT CAGAGGGTCA ACCACAGGCT TGTCTGCCAC CCCTCCTGCT 
TCCTTGCCTG GATCTTTAAC TAATGTGAAA GCATTACAAA AATCTCCAGG ACCTCAGCGT 
GAAAGAAAAT CTTCATCATC TTCTGAAGAC AGGAATAGAA TGAAAACCCT TGGTCGACGG 
GACTCAAGTG ATGATTGGGA GATTCCAGAT GGGCAGATTA CAGTTGGACA AAGGATAGGA 
TCTGGATCAT TTGGAACAGT CTACAAGGGA AAATGGCATG GTGATGTAGC TGTGAAAATG 
TTGAATGTGA CAGCACCCAC ACCTCAGCAG TTACAGGCTT TCAAAAATGA AGTAGGAGTG 
CTCAGGAAAA CGCGACATGT GAATATCCTA CTCTTTATGG GCTATTCAAC AAAACCACAG 
TTGGCCATTG TTACACAGTG GTGTGAAGGC TCCAGTTTAT ATCACCATCT ACATATCATT 
GAGACCAAAT TTGAGATGAT CAAGCTAATA GATATTGCAC GGCAAACAGC ACAAGGCATG 
GATTACTTAC ACGCCAAGTC AATCATCCAC AGAGACCTCA AGAGTAATAA TATATTTCTT 
CATGAAGACC TCACAGTAAA AATAGGGGAT TTTGGTCTGG CTACAGTAAA ATCTCGGTGG 
AGTGGCTCCC ATCAGTTTGA ACAGTTGTCT GGATCTATCT TGTGGATGGC ACCAGAGGTC 
ATTAGAATGC AAGATAAAAA CCCATATAGC TTTCAATCAG ATGTATATGC GTTTGGGATT 
GTTCTGTATG AGTTGATGAC GGGACAATTA CCTTACTCAA ACATCAACAA CAGGGACCAG 
ATAATTTTTA TGGTGGGGCG AGGATACCTG TCTCCAGATC TCAGTAAGGT GCGGAGTAAT 
TGTCCAAAAG CCATGAAGAG GTTAATGGCA GAGTGCCTGA AGAAGAAGAG AGATGAGAGG 
CCACTTTTTC CCCAGATCCT TGCCTCCATT GAACTTCTGG CCCGCTCATT GCCAAAAATT 
CACCGCAGTG CATCTGAACC CTCACTGAAT CGGGCTGGCT TCCAAACGGA GGATTTTAGT 
CTGTATGCTT GTGCTTCTCC AAAAACGCCC ATCCAGGCAG GGGGATATGG AGAATTTGCC 
GCCTTCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020853802.1
CDS	47..2458
Translation	MAALSGGGGGGAEQGPALFNGDMDPEAGAGSASSAEPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESMGNGTDFSVSSSASTDTVTSSSSSSLSVAPSSLAVFQNPTDVSRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRRQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLGESTLTSGSSPSAPPSDSIGPPILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGLRGDGAPLNQLMRCLRKYQSRTPSPLLHSVPSEIVFDFEPGPVFRGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK

Target ORF information:

RefSeq Version	XM_020998143.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020998143.1

ORF Insert Sequence:

ATGGCGGCGC TGAGCGGCGG CGGCGGCGGC GGCGCGGAGC AGGGCCCCGC TCTCTTCAAC 
GGGGACATGG ATCCCGAGGC TGGCGCCGGC TCGGCGTCCT CCGCCGAGCC GGCCATCCCC 
GAGGAGGTGT GGAACATCAA GCAAATGATT AAATTGACAC AGGAACACAT AGAGGCCCTA 
TTGGACAAGT TCGGAGGGGA ACATAATCCA CCATCAATCT ACCTGGAGGC CTATGAAGAA 
TATACCAGCA AGCTAGATGC ACTTCAACAA CGGGAACAAC AATTATTAGA GTCCATGGGG 
AATGGAACTG ATTTTTCTGT TTCTAGCTCA GCTTCAACAG ACACAGTTAC ATCTTCTTCC 
TCTTCTAGCC TTTCAGTGGC ACCTTCATCT CTTGCAGTTT TTCAGAATCC TACAGATGTA 
TCAAGAAGCA ACCCTAAGTC ACCACAAAAA CCAATTGTTA GAGTCTTCCT GCCCAACAAA 
CAGAGGACTG TGGTGCCAGC AAGATGTGGG GTGACAGTCC GAGACAGTCT AAAGAAAGCA 
TTGATGATGA GAGGCCTGAT CCCAGAATGC TGTGCTGTGT ACAGAAGACA GGATGGAGAG 
AAGAAGCCTA TTGGCTGGGA CACTGATATT TCCTGGCTTA CTGGAGAAGA ATTGCACGTA 
GAAGTTTTGG AGAATGTTCC CCTAACGACA CACAATTTTG TACGGAAGAC ATTTTTTACC 
TTAGCATTCT GTGACTTTTG TCGAAAGTTG CTTTTCCAAG GTTTCCGCTG TCAGACATGT 
GGTTACAAAT TTCACCAACG CTGTAGTACA GAGGTGCCAC TGATGTGTGT TAATTATGAC 
CAGCTTGATT TGCTGTTTGT CTCCAAGTTC TTTGAACACC ACCCAATACC GCAGGAGGAG 
GCCTCCTTAG GAGAGAGCAC CCTTACGTCG GGATCATCCC CTTCAGCACC CCCCTCAGAT 
TCTATTGGGC CCCCAATTCT TACCAGTCCA TCGCCTTCAA AATCCATTCC AATCCCACAG 
CCTTTTCGAC CAGCAGATGA GGATCATCGG AACCAGTTTG GGCAACGAGA CCGGTCCTCA 
TCGGCTCCCA ATGTGCATAT AAATACAATA GAACCTGTCA ATATTGATGA CTTGATTAGA 
GACCAAGGGT TACGGGGTGA TGGAGCCCCC TTGAACCAAC TGATGCGCTG TCTTCGGAAA 
TACCAATCCC GGACTCCCAG TCCCCTCCTA CATTCTGTCC CCAGTGAAAT AGTGTTTGAT 
TTTGAGCCTG GCCCAGTGTT CAGAGGGTCA ACCACAGGCT TGTCTGCCAC CCCTCCTGCT 
TCCTTGCCTG GATCTTTAAC TAATGTGAAA GCATTACAAA AATCTCCAGG ACCTCAGCGT 
GAAAGAAAAT CTTCATCATC TTCTGAAGAC AGGAATAGAA TGAAAACCCT TGGTCGACGG 
GACTCAAGTG ATGATTGGGA GATTCCAGAT GGGCAGATTA CAGTTGGACA AAGGATAGGA 
TCTGGATCAT TTGGAACAGT CTACAAGGGA AAATGGCATG GTGATGTAGC TGTGAAAATG 
TTGAATGTGA CAGCACCCAC ACCTCAGCAG TTACAGGCTT TCAAAAATGA AGTAGGAGTG 
CTCAGGAAAA CGCGACATGT GAATATCCTA CTCTTTATGG GCTATTCAAC AAAACCACAG 
TTGGCCATTG TTACACAGTG GTGTGAAGGC TCCAGTTTAT ATCACCATCT ACATATCATT 
GAGACCAAAT TTGAGATGAT CAAGCTAATA GATATTGCAC GGCAAACAGC ACAAGGCATG 
GATTACTTAC ACGCCAAGTC AATCATCCAC AGAGACCTCA AGAGTAATAA TATATTTCTT 
CATGAAGACC TCACAGTAAA AATAGGGGAT TTTGGTCTGG CTACAGTAAA ATCTCGGTGG 
AGTGGCTCCC ATCAGTTTGA ACAGTTGTCT GGATCTATCT TGTGGATGGC ACCAGAGGTC 
ATTAGAATGC AAGATAAAAA CCCATATAGC TTTCAATCAG ATGTATATGC GTTTGGGATT 
GTTCTGTATG AGTTGATGAC GGGACAATTA CCTTACTCAA ACATCAACAA CAGGGACCAG 
ATAATTTTTA TGGTGGGGCG AGGATACCTG TCTCCAGATC TCAGTAAGGT GCGGAGTAAT 
TGTCCAAAAG CCATGAAGAG GTTAATGGCA GAGTGCCTGA AGAAGAAGAG AGATGAGAGG 
CCACTTTTTC CCCAGATCCT TGCCTCCATT GAACTTCTGG CCCGCTCATT GCCAAAAATT 
CACCGCAGTG CATCTGAACC CTCACTGAAT CGGGCTGGCT TCCAAACGGA GGATTTTAGT 
CTGTATGCTT GTGCTTCTCC AAAAACGCCC ATCCAGGCAG GGGGATATGG AGAATTTGCC 
GCCTTCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_020998144.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020853803.1 Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPh263398
Clone ID Related Accession (Same CDS sequence)	XM_020998144.1
Accession Version	XM_020998144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	serine/threonine-protein kinase B-raf isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344022.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC TGAGCGGCGG CGGCGGCGGC GGCGCGGAGC AGGGCCCCGC TCTCTTCAAC 
GGGGACATGG ATCCCGAGGC TGGCGCCGGC TCGGCGTCCT CCGCCGAGCC GGCCATCCCC 
GAGGAGGTGT GGAACATCAA GCAAATGATT AAATTGACAC AGGAACACAT AGAGGCCCTA 
TTGGACAAGT TCGGAGGGGA ACATAATCCA CCATCAATCT ACCTGGAGGC CTATGAAGAA 
TATACCAGCA AGCTAGATGC ACTTCAACAA CGGGAACAAC AATTATTAGA GTCCATGGGG 
AATGGAACTG ATTTTTCTGT TTCTAGCTCA GCTTCAACAG ACACAGTTAC ATCTTCTTCC 
TCTTCTAGCC TTTCAGTGGC ACCTTCATCT CTTGCAGTTT TTCAGAATCC TACAGATGTA 
TCAAGAAGCA ACCCTAAGTC ACCACAAAAA CCAATTGTTA GAGTCTTCCT GCCCAACAAA 
CAGAGGACTG TGGTGCCAGC AAGATGTGGG GTGACAGTCC GAGACAGTCT AAAGAAAGCA 
TTGATGATGA GAGGCCTGAT CCCAGAATGC TGTGCTGTGT ACAGAAGACA GGATGGAGAG 
AAGAAGCCTA TTGGCTGGGA CACTGATATT TCCTGGCTTA CTGGAGAAGA ATTGCACGTA 
GAAGTTTTGG AGAATGTTCC CCTAACGACA CACAATTTTG TACGGAAGAC ATTTTTTACC 
TTAGCATTCT GTGACTTTTG TCGAAAGTTG CTTTTCCAAG GTTTCCGCTG TCAGACATGT 
GGTTACAAAT TTCACCAACG CTGTAGTACA GAGGTGCCAC TGATGTGTGT TAATTATGAC 
CAGCTTGATT TGCTGTTTGT CTCCAAGTTC TTTGAACACC ACCCAATACC GCAGGAGGAG 
GCCTCCTTAG GAGAGAGCAC CCTTACGTCG GGATCATCCC CTTCAGCACC CCCCTCAGAT 
TCTATTGGGC CCCCAATTCT TACCAGTCCA TCGCCTTCAA AATCCATTCC AATCCCACAG 
CCTTTTCGAC CAGCAGATGA GGATCATCGG AACCAGTTTG GGCAACGAGA CCGGTCCTCA 
TCGGCTCCCA ATGTGCATAT AAATACAATA GAACCTGTCA ATATTGATGA CTTGATTAGA 
GACCAAGGGT TACGGGGTGA TGGAGCCCCC TTGAACCAAC TGATGCGCTG TCTTCGGAAA 
TACCAATCCC GGACTCCCAG TCCCCTCCTA CATTCTGTCC CCAGTGAAAT AGTGTTTGAT 
TTTGAGCCTG GCCCAGTGTT CAGAGGGTCA ACCACAGGCT TGTCTGCCAC CCCTCCTGCT 
TCCTTGCCTG GATCTTTAAC TAATGTGAAA GCATTACAAA AATCTCCAGG ACCTCAGCGT 
GAAAGAAAAT CTTCATCATC TTCTGAAGAC AGGAATAGAA TGAAAACCCT TGGTCGACGG 
GACTCAAGTG ATGATTGGGA GATTCCAGAT GGGCAGATTA CAGTTGGACA AAGGATAGGA 
TCTGGATCAT TTGGAACAGT CTACAAGGGA AAATGGCATG GTGATGTAGC TGTGAAAATG 
TTGAATGTGA CAGCACCCAC ACCTCAGCAG TTACAGGCTT TCAAAAATGA AGTAGGAGTG 
CTCAGGAAAA CGCGACATGT GAATATCCTA CTCTTTATGG GCTATTCAAC AAAACCACAG 
TTGGCCATTG TTACACAGTG GTGTGAAGGC TCCAGTTTAT ATCACCATCT ACATATCATT 
GAGACCAAAT TTGAGATGAT CAAGCTAATA GATATTGCAC GGCAAACAGC ACAAGGCATG 
GATTACTTAC ACGCCAAGTC AATCATCCAC AGAGACCTCA AGAGTAATAA TATATTTCTT 
CATGAAGACC TCACAGTAAA AATAGGGGAT TTTGGTCTGG CTACAGTAAA ATCTCGGTGG 
AGTGGCTCCC ATCAGTTTGA ACAGTTGTCT GGATCTATCT TGTGGATGGC ACCAGAGGTC 
ATTAGAATGC AAGATAAAAA CCCATATAGC TTTCAATCAG ATGTATATGC GTTTGGGATT 
GTTCTGTATG AGTTGATGAC GGGACAATTA CCTTACTCAA ACATCAACAA CAGGGACCAG 
ATAATTTTTA TGGTGGGGCG AGGATACCTG TCTCCAGATC TCAGTAAGGT GCGGAGTAAT 
TGTCCAAAAG CCATGAAGAG GTTAATGGCA GAGTGCCTGA AGAAGAAGAG AGATGAGAGG 
CCACTTTTTC CCCAGGAGAA TTTGCCGCCT TCAAGTAGCC ACACCATCAT GGCAGCATCT 
ACTCTTTATT ATTTCTTAAG TCTTGTGTTC CTACAGTTTC TTAACATCAA AACTCAGTTC 
TGTTCCTCAA AACCTTTTTT AAAGATACAG AAATTTCAGT GCATAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020853803.1
CDS	48..2438
Translation	MAALSGGGGGGAEQGPALFNGDMDPEAGAGSASSAEPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESMGNGTDFSVSSSASTDTVTSSSSSSLSVAPSSLAVFQNPTDVSRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRRQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLGESTLTSGSSPSAPPSDSIGPPILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGLRGDGAPLNQLMRCLRKYQSRTPSPLLHSVPSEIVFDFEPGPVFRGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQENLPPSSSHTIMAASTLYYFLSLVFLQFLNIKTQFCSSKPFLKIQKFQCIS

Target ORF information:

RefSeq Version	XM_020998144.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020998144.1

ORF Insert Sequence:

ATGGCGGCGC TGAGCGGCGG CGGCGGCGGC GGCGCGGAGC AGGGCCCCGC TCTCTTCAAC 
GGGGACATGG ATCCCGAGGC TGGCGCCGGC TCGGCGTCCT CCGCCGAGCC GGCCATCCCC 
GAGGAGGTGT GGAACATCAA GCAAATGATT AAATTGACAC AGGAACACAT AGAGGCCCTA 
TTGGACAAGT TCGGAGGGGA ACATAATCCA CCATCAATCT ACCTGGAGGC CTATGAAGAA 
TATACCAGCA AGCTAGATGC ACTTCAACAA CGGGAACAAC AATTATTAGA GTCCATGGGG 
AATGGAACTG ATTTTTCTGT TTCTAGCTCA GCTTCAACAG ACACAGTTAC ATCTTCTTCC 
TCTTCTAGCC TTTCAGTGGC ACCTTCATCT CTTGCAGTTT TTCAGAATCC TACAGATGTA 
TCAAGAAGCA ACCCTAAGTC ACCACAAAAA CCAATTGTTA GAGTCTTCCT GCCCAACAAA 
CAGAGGACTG TGGTGCCAGC AAGATGTGGG GTGACAGTCC GAGACAGTCT AAAGAAAGCA 
TTGATGATGA GAGGCCTGAT CCCAGAATGC TGTGCTGTGT ACAGAAGACA GGATGGAGAG 
AAGAAGCCTA TTGGCTGGGA CACTGATATT TCCTGGCTTA CTGGAGAAGA ATTGCACGTA 
GAAGTTTTGG AGAATGTTCC CCTAACGACA CACAATTTTG TACGGAAGAC ATTTTTTACC 
TTAGCATTCT GTGACTTTTG TCGAAAGTTG CTTTTCCAAG GTTTCCGCTG TCAGACATGT 
GGTTACAAAT TTCACCAACG CTGTAGTACA GAGGTGCCAC TGATGTGTGT TAATTATGAC 
CAGCTTGATT TGCTGTTTGT CTCCAAGTTC TTTGAACACC ACCCAATACC GCAGGAGGAG 
GCCTCCTTAG GAGAGAGCAC CCTTACGTCG GGATCATCCC CTTCAGCACC CCCCTCAGAT 
TCTATTGGGC CCCCAATTCT TACCAGTCCA TCGCCTTCAA AATCCATTCC AATCCCACAG 
CCTTTTCGAC CAGCAGATGA GGATCATCGG AACCAGTTTG GGCAACGAGA CCGGTCCTCA 
TCGGCTCCCA ATGTGCATAT AAATACAATA GAACCTGTCA ATATTGATGA CTTGATTAGA 
GACCAAGGGT TACGGGGTGA TGGAGCCCCC TTGAACCAAC TGATGCGCTG TCTTCGGAAA 
TACCAATCCC GGACTCCCAG TCCCCTCCTA CATTCTGTCC CCAGTGAAAT AGTGTTTGAT 
TTTGAGCCTG GCCCAGTGTT CAGAGGGTCA ACCACAGGCT TGTCTGCCAC CCCTCCTGCT 
TCCTTGCCTG GATCTTTAAC TAATGTGAAA GCATTACAAA AATCTCCAGG ACCTCAGCGT 
GAAAGAAAAT CTTCATCATC TTCTGAAGAC AGGAATAGAA TGAAAACCCT TGGTCGACGG 
GACTCAAGTG ATGATTGGGA GATTCCAGAT GGGCAGATTA CAGTTGGACA AAGGATAGGA 
TCTGGATCAT TTGGAACAGT CTACAAGGGA AAATGGCATG GTGATGTAGC TGTGAAAATG 
TTGAATGTGA CAGCACCCAC ACCTCAGCAG TTACAGGCTT TCAAAAATGA AGTAGGAGTG 
CTCAGGAAAA CGCGACATGT GAATATCCTA CTCTTTATGG GCTATTCAAC AAAACCACAG 
TTGGCCATTG TTACACAGTG GTGTGAAGGC TCCAGTTTAT ATCACCATCT ACATATCATT 
GAGACCAAAT TTGAGATGAT CAAGCTAATA GATATTGCAC GGCAAACAGC ACAAGGCATG 
GATTACTTAC ACGCCAAGTC AATCATCCAC AGAGACCTCA AGAGTAATAA TATATTTCTT 
CATGAAGACC TCACAGTAAA AATAGGGGAT TTTGGTCTGG CTACAGTAAA ATCTCGGTGG 
AGTGGCTCCC ATCAGTTTGA ACAGTTGTCT GGATCTATCT TGTGGATGGC ACCAGAGGTC 
ATTAGAATGC AAGATAAAAA CCCATATAGC TTTCAATCAG ATGTATATGC GTTTGGGATT 
GTTCTGTATG AGTTGATGAC GGGACAATTA CCTTACTCAA ACATCAACAA CAGGGACCAG 
ATAATTTTTA TGGTGGGGCG AGGATACCTG TCTCCAGATC TCAGTAAGGT GCGGAGTAAT 
TGTCCAAAAG CCATGAAGAG GTTAATGGCA GAGTGCCTGA AGAAGAAGAG AGATGAGAGG 
CCACTTTTTC CCCAGGAGAA TTTGCCGCCT TCAAGTAGCC ACACCATCAT GGCAGCATCT 
ACTCTTTATT ATTTCTTAAG TCTTGTGTTC CTACAGTTTC TTAACATCAA AACTCAGTTC 
TGTTCCTCAA AACCTTTTTT AAAGATACAG AAATTTCAGT GCATAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_020998145.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020853804.1 Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPh263399
Clone ID Related Accession (Same CDS sequence)	XM_020998145.1
Accession Version	XM_020998145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	serine/threonine-protein kinase B-raf isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344022.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC TGAGCGGCGG CGGCGGCGGC GGCGCGGAGC AGGGCCCCGC TCTCTTCAAC 
GGGGACATGG ATCCCGAGGC TGGCGCCGGC TCGGCGTCCT CCGCCGAGCC GGCCATCCCC 
GAGGAGGTGT GGAACATCAA GCAAATGATT AAATTGACAC AGGAACACAT AGAGGCCCTA 
TTGGACAAGT TCGGAGGGGA ACATAATCCA CCATCAATCT ACCTGGAGGC CTATGAAGAA 
TATACCAGCA AGCTAGATGC ACTTCAACAA CGGGAACAAC AATTATTAGA GTCCATGGGG 
AATGGAACTG ATTTTTCTGT TTCTAGCTCA GCTTCAACAG ACACAGTTAC ATCTTCTTCC 
TCTTCTAGCC TTTCAGTGGC ACCTTCATCT CTTGCAGTTT TTCAGAATCC TACAGATGTA 
TCAAGAAGCA ACCCTAAGTC ACCACAAAAA CCAATTGTTA GAGTCTTCCT GCCCAACAAA 
CAGAGGACTG TGGTGCCAGC AAGATGTGGG GTGACAGTCC GAGACAGTCT AAAGAAAGCA 
TTGATGATGA GAGGCCTGAT CCCAGAATGC TGTGCTGTGT ACAGAAGACA GGATGGAGAG 
AAGAAGCCTA TTGGCTGGGA CACTGATATT TCCTGGCTTA CTGGAGAAGA ATTGCACGTA 
GAAGTTTTGG AGAATGTTCC CCTAACGACA CACAATTTTG TACGGAAGAC ATTTTTTACC 
TTAGCATTCT GTGACTTTTG TCGAAAGTTG CTTTTCCAAG GTTTCCGCTG TCAGACATGT 
GGTTACAAAT TTCACCAACG CTGTAGTACA GAGGTGCCAC TGATGTGTGT TAATTATGAC 
CAGCTTGATT TGCTGTTTGT CTCCAAGTTC TTTGAACACC ACCCAATACC GCAGGAGGAG 
GCCTCCTTAG GAGAGAGCAC CCTTACGTCG GGATCATCCC CTTCAGCACC CCCCTCAGAT 
TCTATTGGGC CCCCAATTCT TACCAGTCCA TCGCCTTCAA AATCCATTCC AATCCCACAG 
CCTTTTCGAC CAGCAGATGA GGATCATCGG AACCAGTTTG GGCAACGAGA CCGGTCCTCA 
TCGGCTCCCA ATGTGCATAT AAATACAATA GAACCTGTCA ATATTGATGA CTTGATTAGA 
GACCAAGGGT TACGGGGTGA TGGAGGGTCA ACCACAGGCT TGTCTGCCAC CCCTCCTGCT 
TCCTTGCCTG GATCTTTAAC TAATGTGAAA GCATTACAAA AATCTCCAGG ACCTCAGCGT 
GAAAGAAAAT CTTCATCATC TTCTGAAGAC AGGAATAGAA TGAAAACCCT TGGTCGACGG 
GACTCAAGTG ATGATTGGGA GATTCCAGAT GGGCAGATTA CAGTTGGACA AAGGATAGGA 
TCTGGATCAT TTGGAACAGT CTACAAGGGA AAATGGCATG GTGATGTAGC TGTGAAAATG 
TTGAATGTGA CAGCACCCAC ACCTCAGCAG TTACAGGCTT TCAAAAATGA AGTAGGAGTG 
CTCAGGAAAA CGCGACATGT GAATATCCTA CTCTTTATGG GCTATTCAAC AAAACCACAG 
TTGGCCATTG TTACACAGTG GTGTGAAGGC TCCAGTTTAT ATCACCATCT ACATATCATT 
GAGACCAAAT TTGAGATGAT CAAGCTAATA GATATTGCAC GGCAAACAGC ACAAGGCATG 
GATTACTTAC ACGCCAAGTC AATCATCCAC AGAGACCTCA AGAGTAATAA TATATTTCTT 
CATGAAGACC TCACAGTAAA AATAGGGGAT TTTGGTCTGG CTACAGTAAA ATCTCGGTGG 
AGTGGCTCCC ATCAGTTTGA ACAGTTGTCT GGATCTATCT TGTGGATGGC ACCAGAGGTC 
ATTAGAATGC AAGATAAAAA CCCATATAGC TTTCAATCAG ATGTATATGC GTTTGGGATT 
GTTCTGTATG AGTTGATGAC GGGACAATTA CCTTACTCAA ACATCAACAA CAGGGACCAG 
ATAATTTTTA TGGTGGGGCG AGGATACCTG TCTCCAGATC TCAGTAAGGT GCGGAGTAAT 
TGTCCAAAAG CCATGAAGAG GTTAATGGCA GAGTGCCTGA AGAAGAAGAG AGATGAGAGG 
CCACTTTTTC CCCAGATCCT TGCCTCCATT GAACTTCTGG CCCGCTCATT GCCAAAAATT 
CACCGCAGTG CATCTGAACC CTCACTGAAT CGGGCTGGCT TCCAAACGGA GGATTTTAGT 
CTGTATGCTT GTGCTTCTCC AAAAACGCCC ATCCAGGCAG GGGGATATGG AGAATTTGCC 
GCCTTCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020853804.1
CDS	46..2337
Translation	MAALSGGGGGGAEQGPALFNGDMDPEAGAGSASSAEPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESMGNGTDFSVSSSASTDTVTSSSSSSLSVAPSSLAVFQNPTDVSRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRRQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLGESTLTSGSSPSAPPSDSIGPPILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGLRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK

Target ORF information:

RefSeq Version	XM_020998145.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020998145.1

ORF Insert Sequence:

ATGGCGGCGC TGAGCGGCGG CGGCGGCGGC GGCGCGGAGC AGGGCCCCGC TCTCTTCAAC 
GGGGACATGG ATCCCGAGGC TGGCGCCGGC TCGGCGTCCT CCGCCGAGCC GGCCATCCCC 
GAGGAGGTGT GGAACATCAA GCAAATGATT AAATTGACAC AGGAACACAT AGAGGCCCTA 
TTGGACAAGT TCGGAGGGGA ACATAATCCA CCATCAATCT ACCTGGAGGC CTATGAAGAA 
TATACCAGCA AGCTAGATGC ACTTCAACAA CGGGAACAAC AATTATTAGA GTCCATGGGG 
AATGGAACTG ATTTTTCTGT TTCTAGCTCA GCTTCAACAG ACACAGTTAC ATCTTCTTCC 
TCTTCTAGCC TTTCAGTGGC ACCTTCATCT CTTGCAGTTT TTCAGAATCC TACAGATGTA 
TCAAGAAGCA ACCCTAAGTC ACCACAAAAA CCAATTGTTA GAGTCTTCCT GCCCAACAAA 
CAGAGGACTG TGGTGCCAGC AAGATGTGGG GTGACAGTCC GAGACAGTCT AAAGAAAGCA 
TTGATGATGA GAGGCCTGAT CCCAGAATGC TGTGCTGTGT ACAGAAGACA GGATGGAGAG 
AAGAAGCCTA TTGGCTGGGA CACTGATATT TCCTGGCTTA CTGGAGAAGA ATTGCACGTA 
GAAGTTTTGG AGAATGTTCC CCTAACGACA CACAATTTTG TACGGAAGAC ATTTTTTACC 
TTAGCATTCT GTGACTTTTG TCGAAAGTTG CTTTTCCAAG GTTTCCGCTG TCAGACATGT 
GGTTACAAAT TTCACCAACG CTGTAGTACA GAGGTGCCAC TGATGTGTGT TAATTATGAC 
CAGCTTGATT TGCTGTTTGT CTCCAAGTTC TTTGAACACC ACCCAATACC GCAGGAGGAG 
GCCTCCTTAG GAGAGAGCAC CCTTACGTCG GGATCATCCC CTTCAGCACC CCCCTCAGAT 
TCTATTGGGC CCCCAATTCT TACCAGTCCA TCGCCTTCAA AATCCATTCC AATCCCACAG 
CCTTTTCGAC CAGCAGATGA GGATCATCGG AACCAGTTTG GGCAACGAGA CCGGTCCTCA 
TCGGCTCCCA ATGTGCATAT AAATACAATA GAACCTGTCA ATATTGATGA CTTGATTAGA 
GACCAAGGGT TACGGGGTGA TGGAGGGTCA ACCACAGGCT TGTCTGCCAC CCCTCCTGCT 
TCCTTGCCTG GATCTTTAAC TAATGTGAAA GCATTACAAA AATCTCCAGG ACCTCAGCGT 
GAAAGAAAAT CTTCATCATC TTCTGAAGAC AGGAATAGAA TGAAAACCCT TGGTCGACGG 
GACTCAAGTG ATGATTGGGA GATTCCAGAT GGGCAGATTA CAGTTGGACA AAGGATAGGA 
TCTGGATCAT TTGGAACAGT CTACAAGGGA AAATGGCATG GTGATGTAGC TGTGAAAATG 
TTGAATGTGA CAGCACCCAC ACCTCAGCAG TTACAGGCTT TCAAAAATGA AGTAGGAGTG 
CTCAGGAAAA CGCGACATGT GAATATCCTA CTCTTTATGG GCTATTCAAC AAAACCACAG 
TTGGCCATTG TTACACAGTG GTGTGAAGGC TCCAGTTTAT ATCACCATCT ACATATCATT 
GAGACCAAAT TTGAGATGAT CAAGCTAATA GATATTGCAC GGCAAACAGC ACAAGGCATG 
GATTACTTAC ACGCCAAGTC AATCATCCAC AGAGACCTCA AGAGTAATAA TATATTTCTT 
CATGAAGACC TCACAGTAAA AATAGGGGAT TTTGGTCTGG CTACAGTAAA ATCTCGGTGG 
AGTGGCTCCC ATCAGTTTGA ACAGTTGTCT GGATCTATCT TGTGGATGGC ACCAGAGGTC 
ATTAGAATGC AAGATAAAAA CCCATATAGC TTTCAATCAG ATGTATATGC GTTTGGGATT 
GTTCTGTATG AGTTGATGAC GGGACAATTA CCTTACTCAA ACATCAACAA CAGGGACCAG 
ATAATTTTTA TGGTGGGGCG AGGATACCTG TCTCCAGATC TCAGTAAGGT GCGGAGTAAT 
TGTCCAAAAG CCATGAAGAG GTTAATGGCA GAGTGCCTGA AGAAGAAGAG AGATGAGAGG 
CCACTTTTTC CCCAGATCCT TGCCTCCATT GAACTTCTGG CCCGCTCATT GCCAAAAATT 
CACCGCAGTG CATCTGAACC CTCACTGAAT CGGGCTGGCT TCCAAACGGA GGATTTTAGT 
CTGTATGCTT GTGCTTCTCC AAAAACGCCC ATCCAGGCAG GGGGATATGG AGAATTTGCC 
GCCTTCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_020998146.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020853805.1 Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPh263400
Clone ID Related Accession (Same CDS sequence)	XM_020998146.1
Accession Version	XM_020998146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	serine/threonine-protein kinase B-raf isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344022.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC TGAGCGGCGG CGGCGGCGGC GGCGCGGAGC AGGGCCCCGC TCTCTTCAAC 
GGGGACATGG ATCCCGAGGC TGGCGCCGGC TCGGCGTCCT CCGCCGAGCC GGCCATCCCC 
GAGGAGGTGT GGAACATCAA GCAAATGATT AAATTGACAC AGGAACACAT AGAGGCCCTA 
TTGGACAAGT TCGGAGGGGA ACATAATCCA CCATCAATCT ACCTGGAGGC CTATGAAGAA 
TATACCAGCA AGCTAGATGC ACTTCAACAA CGGGAACAAC AATTATTAGA GTCCATGGGG 
AATGGAACTG ATTTTTCTGT TTCTAGCTCA GCTTCAACAG ACACAGTTAC ATCTTCTTCC 
TCTTCTAGCC TTTCAGTGGC ACCTTCATCT CTTGCAGTTT TTCAGAATCC TACAGATGTA 
TCAAGAAGCA ACCCTAAGTC ACCACAAAAA CCAATTGTTA GAGTCTTCCT GCCCAACAAA 
CAGAGGACTG TGGTGCCAGC AAGATGTGGG GTGACAGTCC GAGACAGTCT AAAGAAAGCA 
TTGATGATGA GAGGCCTGAT CCCAGAATGC TGTGCTGTGT ACAGAAGACA GGATGGAGAG 
AAGAAGCCTA TTGGCTGGGA CACTGATATT TCCTGGCTTA CTGGAGAAGA ATTGCACGTA 
GAAGTTTTGG AGAATGTTCC CCTAACGACA CACAATTTTG TACGGAAGAC ATTTTTTACC 
TTAGCATTCT GTGACTTTTG TCGAAAGTTG CTTTTCCAAG GTTTCCGCTG TCAGACATGT 
GGTTACAAAT TTCACCAACG CTGTAGTACA GAGGTGCCAC TGATGTGTGT TAATTATGAC 
CAGCTTGAGC CCCCAATTCT TACCAGTCCA TCGCCTTCAA AATCCATTCC AATCCCACAG 
CCTTTTCGAC CAGCAGATGA GGATCATCGG AACCAGTTTG GGCAACGAGA CCGGTCCTCA 
TCGGCTCCCA ATGTGCATAT AAATACAATA GAACCTGTCA ATATTGATGA CTTGATTAGA 
GACCAAGGGT TACGGGGTGA TGGAGCCCCC TTGAACCAAC TGATGCGCTG TCTTCGGAAA 
TACCAATCCC GGACTCCCAG TCCCCTCCTA CATTCTGTCC CCAGTGAAAT AGTGTTTGAT 
TTTGAGCCTG GCCCAGTGTT CAGAGGGTCA ACCACAGGCT TGTCTGCCAC CCCTCCTGCT 
TCCTTGCCTG GATCTTTAAC TAATGTGAAA GCATTACAAA AATCTCCAGG ACCTCAGCGT 
GAAAGAAAAT CTTCATCATC TTCTGAAGAC AGGAATAGAA TGAAAACCCT TGGTCGACGG 
GACTCAAGTG ATGATTGGGA GATTCCAGAT GGGCAGATTA CAGTTGGACA AAGGATAGGA 
TCTGGATCAT TTGGAACAGT CTACAAGGGA AAATGGCATG GTGATGTAGC TGTGAAAATG 
TTGAATGTGA CAGCACCCAC ACCTCAGCAG TTACAGGCTT TCAAAAATGA AGTAGGAGTG 
CTCAGGAAAA CGCGACATGT GAATATCCTA CTCTTTATGG GCTATTCAAC AAAACCACAG 
TTGGCCATTG TTACACAGTG GTGTGAAGGC TCCAGTTTAT ATCACCATCT ACATATCATT 
GAGACCAAAT TTGAGATGAT CAAGCTAATA GATATTGCAC GGCAAACAGC ACAAGGCATG 
GATTACTTAC ACGCCAAGTC AATCATCCAC AGAGACCTCA AGAGTAATAA TATATTTCTT 
CATGAAGACC TCACAGTAAA AATAGGGGAT TTTGGTCTGG CTACAGTAAA ATCTCGGTGG 
AGTGGCTCCC ATCAGTTTGA ACAGTTGTCT GGATCTATCT TGTGGATGGC ACCAGAGGTC 
ATTAGAATGC AAGATAAAAA CCCATATAGC TTTCAATCAG ATGTATATGC GTTTGGGATT 
GTTCTGTATG AGTTGATGAC GGGACAATTA CCTTACTCAA ACATCAACAA CAGGGACCAG 
ATAATTTTTA TGGTGGGGCG AGGATACCTG TCTCCAGATC TCAGTAAGGT GCGGAGTAAT 
TGTCCAAAAG CCATGAAGAG GTTAATGGCA GAGTGCCTGA AGAAGAAGAG AGATGAGAGG 
CCACTTTTTC CCCAGATCCT TGCCTCCATT GAACTTCTGG CCCGCTCATT GCCAAAAATT 
CACCGCAGTG CATCTGAACC CTCACTGAAT CGGGCTGGCT TCCAAACGGA GGATTTTAGT 
CTGTATGCTT GTGCTTCTCC AAAAACGCCC ATCCAGGCAG GGGGATATGG AGAATTTGCC 
GCCTTCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020853805.1
CDS	48..2339
Translation	MAALSGGGGGGAEQGPALFNGDMDPEAGAGSASSAEPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESMGNGTDFSVSSSASTDTVTSSSSSSLSVAPSSLAVFQNPTDVSRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRRQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLEPPILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGLRGDGAPLNQLMRCLRKYQSRTPSPLLHSVPSEIVFDFEPGPVFRGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK

Target ORF information:

RefSeq Version	XM_020998146.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020998146.1

ORF Insert Sequence:

ATGGCGGCGC TGAGCGGCGG CGGCGGCGGC GGCGCGGAGC AGGGCCCCGC TCTCTTCAAC 
GGGGACATGG ATCCCGAGGC TGGCGCCGGC TCGGCGTCCT CCGCCGAGCC GGCCATCCCC 
GAGGAGGTGT GGAACATCAA GCAAATGATT AAATTGACAC AGGAACACAT AGAGGCCCTA 
TTGGACAAGT TCGGAGGGGA ACATAATCCA CCATCAATCT ACCTGGAGGC CTATGAAGAA 
TATACCAGCA AGCTAGATGC ACTTCAACAA CGGGAACAAC AATTATTAGA GTCCATGGGG 
AATGGAACTG ATTTTTCTGT TTCTAGCTCA GCTTCAACAG ACACAGTTAC ATCTTCTTCC 
TCTTCTAGCC TTTCAGTGGC ACCTTCATCT CTTGCAGTTT TTCAGAATCC TACAGATGTA 
TCAAGAAGCA ACCCTAAGTC ACCACAAAAA CCAATTGTTA GAGTCTTCCT GCCCAACAAA 
CAGAGGACTG TGGTGCCAGC AAGATGTGGG GTGACAGTCC GAGACAGTCT AAAGAAAGCA 
TTGATGATGA GAGGCCTGAT CCCAGAATGC TGTGCTGTGT ACAGAAGACA GGATGGAGAG 
AAGAAGCCTA TTGGCTGGGA CACTGATATT TCCTGGCTTA CTGGAGAAGA ATTGCACGTA 
GAAGTTTTGG AGAATGTTCC CCTAACGACA CACAATTTTG TACGGAAGAC ATTTTTTACC 
TTAGCATTCT GTGACTTTTG TCGAAAGTTG CTTTTCCAAG GTTTCCGCTG TCAGACATGT 
GGTTACAAAT TTCACCAACG CTGTAGTACA GAGGTGCCAC TGATGTGTGT TAATTATGAC 
CAGCTTGAGC CCCCAATTCT TACCAGTCCA TCGCCTTCAA AATCCATTCC AATCCCACAG 
CCTTTTCGAC CAGCAGATGA GGATCATCGG AACCAGTTTG GGCAACGAGA CCGGTCCTCA 
TCGGCTCCCA ATGTGCATAT AAATACAATA GAACCTGTCA ATATTGATGA CTTGATTAGA 
GACCAAGGGT TACGGGGTGA TGGAGCCCCC TTGAACCAAC TGATGCGCTG TCTTCGGAAA 
TACCAATCCC GGACTCCCAG TCCCCTCCTA CATTCTGTCC CCAGTGAAAT AGTGTTTGAT 
TTTGAGCCTG GCCCAGTGTT CAGAGGGTCA ACCACAGGCT TGTCTGCCAC CCCTCCTGCT 
TCCTTGCCTG GATCTTTAAC TAATGTGAAA GCATTACAAA AATCTCCAGG ACCTCAGCGT 
GAAAGAAAAT CTTCATCATC TTCTGAAGAC AGGAATAGAA TGAAAACCCT TGGTCGACGG 
GACTCAAGTG ATGATTGGGA GATTCCAGAT GGGCAGATTA CAGTTGGACA AAGGATAGGA 
TCTGGATCAT TTGGAACAGT CTACAAGGGA AAATGGCATG GTGATGTAGC TGTGAAAATG 
TTGAATGTGA CAGCACCCAC ACCTCAGCAG TTACAGGCTT TCAAAAATGA AGTAGGAGTG 
CTCAGGAAAA CGCGACATGT GAATATCCTA CTCTTTATGG GCTATTCAAC AAAACCACAG 
TTGGCCATTG TTACACAGTG GTGTGAAGGC TCCAGTTTAT ATCACCATCT ACATATCATT 
GAGACCAAAT TTGAGATGAT CAAGCTAATA GATATTGCAC GGCAAACAGC ACAAGGCATG 
GATTACTTAC ACGCCAAGTC AATCATCCAC AGAGACCTCA AGAGTAATAA TATATTTCTT 
CATGAAGACC TCACAGTAAA AATAGGGGAT TTTGGTCTGG CTACAGTAAA ATCTCGGTGG 
AGTGGCTCCC ATCAGTTTGA ACAGTTGTCT GGATCTATCT TGTGGATGGC ACCAGAGGTC 
ATTAGAATGC AAGATAAAAA CCCATATAGC TTTCAATCAG ATGTATATGC GTTTGGGATT 
GTTCTGTATG AGTTGATGAC GGGACAATTA CCTTACTCAA ACATCAACAA CAGGGACCAG 
ATAATTTTTA TGGTGGGGCG AGGATACCTG TCTCCAGATC TCAGTAAGGT GCGGAGTAAT 
TGTCCAAAAG CCATGAAGAG GTTAATGGCA GAGTGCCTGA AGAAGAAGAG AGATGAGAGG 
CCACTTTTTC CCCAGATCCT TGCCTCCATT GAACTTCTGG CCCGCTCATT GCCAAAAATT 
CACCGCAGTG CATCTGAACC CTCACTGAAT CGGGCTGGCT TCCAAACGGA GGATTTTAGT 
CTGTATGCTT GTGCTTCTCC AAAAACGCCC ATCCAGGCAG GGGGATATGG AGAATTTGCC 
GCCTTCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_020998147.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020853806.1 Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPh263401
Clone ID Related Accession (Same CDS sequence)	XM_020998147.1
Accession Version	XM_020998147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	serine/threonine-protein kinase B-raf isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344022.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC TGAGCGGCGG CGGCGGCGGC GGCGCGGAGC AGGGCCCCGC TCTCTTCAAC 
GGGGACATGG ATCCCGAGGC TGGCGCCGGC TCGGCGTCCT CCGCCGAGCC GGCCATCCCC 
GAGGAGGTGT GGAACATCAA GCAAATGATT AAATTGACAC AGGAACACAT AGAGGCCCTA 
TTGGACAAGT TCGGAGGGGA ACATAATCCA CCATCAATCT ACCTGGAGGC CTATGAAGAA 
TATACCAGCA AGCTAGATGC ACTTCAACAA CGGGAACAAC AATTATTAGA GTCCATGGGG 
AATGGAACTG ATTTTTCTGT TTCTAGCTCA GCTTCAACAG ACACAGTTAC ATCTTCTTCC 
TCTTCTAGCC TTTCAGTGGC ACCTTCATCT CTTGCAGTTT TTCAGAATCC TACAGATGTA 
TCAAGAAGCA ACCCTAAGTC ACCACAAAAA CCAATTGTTA GAGTCTTCCT GCCCAACAAA 
CAGAGGACTG TGGTGCCAGC AAGATGTGGG GTGACAGTCC GAGACAGTCT AAAGAAAGCA 
TTGATGATGA GAGGCCTGAT CCCAGAATGC TGTGCTGTGT ACAGAAGACA GGATGGAGAG 
AAGAAGCCTA TTGGCTGGGA CACTGATATT TCCTGGCTTA CTGGAGAAGA ATTGCACGTA 
GAAGTTTTGG AGAATGTTCC CCTAACGACA CACAATTTTG TACGGAAGAC ATTTTTTACC 
TTAGCATTCT GTGACTTTTG TCGAAAGTTG CTTTTCCAAG GTTTCCGCTG TCAGACATGT 
GGTTACAAAT TTCACCAACG CTGTAGTACA GAGGTGCCAC TGATGTGTGT TAATTATGAC 
CAGCTTGATT TGCTGTTTGT CTCCAAGTTC TTTGAACACC ACCCAATACC GCAGGAGGAG 
GCCTCCTTAG GAGAGAGCAC CCTTACGTCG GGATCATCCC CTTCAGCACC CCCCTCAGAT 
TCTATTGGGC CCCCAATTCT TACCAGTCCA TCGCCTTCAA AATCCATTCC AATCCCACAG 
CCTTTTCGAC CAGCAGATGA GGATCATCGG AACCAGTTTG GGCAACGAGA CCGGTCCTCA 
TCGGCTCCCA ATGTGCATAT AAATACAATA GAACCTGTCA ATATTGATGA CTTGATTAGA 
GACCAAGGGT TACGGGGTGA TGGAGGGTCA ACCACAGGCT TGTCTGCCAC CCCTCCTGCT 
TCCTTGCCTG GATCTTTAAC TAATGTGAAA GCATTACAAA AATCTCCAGG ACCTCAGCGT 
GAAAGAAAAT CTTCATCATC TTCTGAAGAC AGGAATAGAA TGAAAACCCT TGGTCGACGG 
GACTCAAGTG ATGATTGGGA GATTCCAGAT GGGCAGATTA CAGTTGGACA AAGGATAGGA 
TCTGGATCAT TTGGAACAGT CTACAAGGGA AAATGGCATG GTGATGTAGC TGTGAAAATG 
TTGAATGTGA CAGCACCCAC ACCTCAGCAG TTACAGGCTT TCAAAAATGA AGTAGGAGTG 
CTCAGGAAAA CGCGACATGT GAATATCCTA CTCTTTATGG GCTATTCAAC AAAACCACAG 
TTGGCCATTG TTACACAGTG GTGTGAAGGC TCCAGTTTAT ATCACCATCT ACATATCATT 
GAGACCAAAT TTGAGATGAT CAAGCTAATA GATATTGCAC GGCAAACAGC ACAAGGCATG 
GATTACTTAC ACGCCAAGTC AATCATCCAC AGAGACCTCA AGAGTAATAA TATATTTCTT 
CATGAAGACC TCACAGTAAA AATAGGGGAT TTTGGTCTGG CTACAGTAAA ATCTCGGTGG 
AGTGGCTCCC ATCAGTTTGA ACAGTTGTCT GGATCTATCT TGTGGATGGC ACCAGAGGTC 
ATTAGAATGC AAGATAAAAA CCCATATAGC TTTCAATCAG ATGTATATGC GTTTGGGATT 
GTTCTGTATG AGTTGATGAC GGGACAATTA CCTTACTCAA ACATCAACAA CAGGGACCAG 
ATAATTTTTA TGGTGGGGCG AGGATACCTG TCTCCAGATC TCAGTAAGGT GCGGAGTAAT 
TGTCCAAAAG CCATGAAGAG GTTAATGGCA GAGTGCCTGA AGAAGAAGAG AGATGAGAGG 
CCACTTTTTC CCCAGATCCT TGCCTCCATT GAACTTCTGG CCCGCTCATT GCCAAAAATT 
CACCGCAGTG CATCTGAACC CTCACTGAAT CGGGCTGGCT TCCAAACGGA GGATTTTAGT 
CTGTATGCTT GTGCTTCTCC AAAAACGCCC ATCCAGGCAG GGGGATATGG TGCGTTTCCT 
GTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020853806.1
CDS	46..2334
Translation	MAALSGGGGGGAEQGPALFNGDMDPEAGAGSASSAEPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESMGNGTDFSVSSSASTDTVTSSSSSSLSVAPSSLAVFQNPTDVSRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRRQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLGESTLTSGSSPSAPPSDSIGPPILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGLRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH

Target ORF information:

RefSeq Version	XM_020998147.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020998147.1

ORF Insert Sequence:

ATGGCGGCGC TGAGCGGCGG CGGCGGCGGC GGCGCGGAGC AGGGCCCCGC TCTCTTCAAC 
GGGGACATGG ATCCCGAGGC TGGCGCCGGC TCGGCGTCCT CCGCCGAGCC GGCCATCCCC 
GAGGAGGTGT GGAACATCAA GCAAATGATT AAATTGACAC AGGAACACAT AGAGGCCCTA 
TTGGACAAGT TCGGAGGGGA ACATAATCCA CCATCAATCT ACCTGGAGGC CTATGAAGAA 
TATACCAGCA AGCTAGATGC ACTTCAACAA CGGGAACAAC AATTATTAGA GTCCATGGGG 
AATGGAACTG ATTTTTCTGT TTCTAGCTCA GCTTCAACAG ACACAGTTAC ATCTTCTTCC 
TCTTCTAGCC TTTCAGTGGC ACCTTCATCT CTTGCAGTTT TTCAGAATCC TACAGATGTA 
TCAAGAAGCA ACCCTAAGTC ACCACAAAAA CCAATTGTTA GAGTCTTCCT GCCCAACAAA 
CAGAGGACTG TGGTGCCAGC AAGATGTGGG GTGACAGTCC GAGACAGTCT AAAGAAAGCA 
TTGATGATGA GAGGCCTGAT CCCAGAATGC TGTGCTGTGT ACAGAAGACA GGATGGAGAG 
AAGAAGCCTA TTGGCTGGGA CACTGATATT TCCTGGCTTA CTGGAGAAGA ATTGCACGTA 
GAAGTTTTGG AGAATGTTCC CCTAACGACA CACAATTTTG TACGGAAGAC ATTTTTTACC 
TTAGCATTCT GTGACTTTTG TCGAAAGTTG CTTTTCCAAG GTTTCCGCTG TCAGACATGT 
GGTTACAAAT TTCACCAACG CTGTAGTACA GAGGTGCCAC TGATGTGTGT TAATTATGAC 
CAGCTTGATT TGCTGTTTGT CTCCAAGTTC TTTGAACACC ACCCAATACC GCAGGAGGAG 
GCCTCCTTAG GAGAGAGCAC CCTTACGTCG GGATCATCCC CTTCAGCACC CCCCTCAGAT 
TCTATTGGGC CCCCAATTCT TACCAGTCCA TCGCCTTCAA AATCCATTCC AATCCCACAG 
CCTTTTCGAC CAGCAGATGA GGATCATCGG AACCAGTTTG GGCAACGAGA CCGGTCCTCA 
TCGGCTCCCA ATGTGCATAT AAATACAATA GAACCTGTCA ATATTGATGA CTTGATTAGA 
GACCAAGGGT TACGGGGTGA TGGAGGGTCA ACCACAGGCT TGTCTGCCAC CCCTCCTGCT 
TCCTTGCCTG GATCTTTAAC TAATGTGAAA GCATTACAAA AATCTCCAGG ACCTCAGCGT 
GAAAGAAAAT CTTCATCATC TTCTGAAGAC AGGAATAGAA TGAAAACCCT TGGTCGACGG 
GACTCAAGTG ATGATTGGGA GATTCCAGAT GGGCAGATTA CAGTTGGACA AAGGATAGGA 
TCTGGATCAT TTGGAACAGT CTACAAGGGA AAATGGCATG GTGATGTAGC TGTGAAAATG 
TTGAATGTGA CAGCACCCAC ACCTCAGCAG TTACAGGCTT TCAAAAATGA AGTAGGAGTG 
CTCAGGAAAA CGCGACATGT GAATATCCTA CTCTTTATGG GCTATTCAAC AAAACCACAG 
TTGGCCATTG TTACACAGTG GTGTGAAGGC TCCAGTTTAT ATCACCATCT ACATATCATT 
GAGACCAAAT TTGAGATGAT CAAGCTAATA GATATTGCAC GGCAAACAGC ACAAGGCATG 
GATTACTTAC ACGCCAAGTC AATCATCCAC AGAGACCTCA AGAGTAATAA TATATTTCTT 
CATGAAGACC TCACAGTAAA AATAGGGGAT TTTGGTCTGG CTACAGTAAA ATCTCGGTGG 
AGTGGCTCCC ATCAGTTTGA ACAGTTGTCT GGATCTATCT TGTGGATGGC ACCAGAGGTC 
ATTAGAATGC AAGATAAAAA CCCATATAGC TTTCAATCAG ATGTATATGC GTTTGGGATT 
GTTCTGTATG AGTTGATGAC GGGACAATTA CCTTACTCAA ACATCAACAA CAGGGACCAG 
ATAATTTTTA TGGTGGGGCG AGGATACCTG TCTCCAGATC TCAGTAAGGT GCGGAGTAAT 
TGTCCAAAAG CCATGAAGAG GTTAATGGCA GAGTGCCTGA AGAAGAAGAG AGATGAGAGG 
CCACTTTTTC CCCAGATCCT TGCCTCCATT GAACTTCTGG CCCGCTCATT GCCAAAAATT 
CACCGCAGTG CATCTGAACC CTCACTGAAT CGGGCTGGCT TCCAAACGGA GGATTTTAGT 
CTGTATGCTT GTGCTTCTCC AAAAACGCCC ATCCAGGCAG GGGGATATGG TGCGTTTCCT 
GTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_020998148.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020853807.1 Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPh263402
Clone ID Related Accession (Same CDS sequence)	XM_020998148.1
Accession Version	XM_020998148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	serine/threonine-protein kinase B-raf isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344022.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTAAAT TGACACAGGA ACACATAGAG GCCCTATTGG ACAAGTTCGG AGGGGAACAT 
AATCCACCAT CAATCTACCT GGAGGCCTAT GAAGAATATA CCAGCAAGCT AGATGCACTT 
CAACAACGGG AACAACAATT ATTAGAGTCC ATGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCAGCTT CAACAGACAC AGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGGCACCT 
TCATCTCTTG CAGTTTTTCA GAATCCTACA GATGTATCAA GAAGCAACCC TAAGTCACCA 
CAAAAACCAA TTGTTAGAGT CTTCCTGCCC AACAAACAGA GGACTGTGGT GCCAGCAAGA 
TGTGGGGTGA CAGTCCGAGA CAGTCTAAAG AAAGCATTGA TGATGAGAGG CCTGATCCCA 
GAATGCTGTG CTGTGTACAG AAGACAGGAT GGAGAGAAGA AGCCTATTGG CTGGGACACT 
GATATTTCCT GGCTTACTGG AGAAGAATTG CACGTAGAAG TTTTGGAGAA TGTTCCCCTA 
ACGACACACA ATTTTGTACG GAAGACATTT TTTACCTTAG CATTCTGTGA CTTTTGTCGA 
AAGTTGCTTT TCCAAGGTTT CCGCTGTCAG ACATGTGGTT ACAAATTTCA CCAACGCTGT 
AGTACAGAGG TGCCACTGAT GTGTGTTAAT TATGACCAGC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCGCAG GAGGAGGCCT CCTTAGGAGA GAGCACCCTT 
ACGTCGGGAT CATCCCCTTC AGCACCCCCC TCAGATTCTA TTGGGCCCCC AATTCTTACC 
AGTCCATCGC CTTCAAAATC CATTCCAATC CCACAGCCTT TTCGACCAGC AGATGAGGAT 
CATCGGAACC AGTTTGGGCA ACGAGACCGG TCCTCATCGG CTCCCAATGT GCATATAAAT 
ACAATAGAAC CTGTCAATAT TGATGACTTG ATTAGAGACC AAGGGTTACG GGGTGATGGA 
GCCCCCTTGA ACCAACTGAT GCGCTGTCTT CGGAAATACC AATCCCGGAC TCCCAGTCCC 
CTCCTACATT CTGTCCCCAG TGAAATAGTG TTTGATTTTG AGCCTGGCCC AGTGTTCAGA 
GGGTCAACCA CAGGCTTGTC TGCCACCCCT CCTGCTTCCT TGCCTGGATC TTTAACTAAT 
GTGAAAGCAT TACAAAAATC TCCAGGACCT CAGCGTGAAA GAAAATCTTC ATCATCTTCT 
GAAGACAGGA ATAGAATGAA AACCCTTGGT CGACGGGACT CAAGTGATGA TTGGGAGATT 
CCAGATGGGC AGATTACAGT TGGACAAAGG ATAGGATCTG GATCATTTGG AACAGTCTAC 
AAGGGAAAAT GGCATGGTGA TGTAGCTGTG AAAATGTTGA ATGTGACAGC ACCCACACCT 
CAGCAGTTAC AGGCTTTCAA AAATGAAGTA GGAGTGCTCA GGAAAACGCG ACATGTGAAT 
ATCCTACTCT TTATGGGCTA TTCAACAAAA CCACAGTTGG CCATTGTTAC ACAGTGGTGT 
GAAGGCTCCA GTTTATATCA CCATCTACAT ATCATTGAGA CCAAATTTGA GATGATCAAG 
CTAATAGATA TTGCACGGCA AACAGCACAA GGCATGGATT ACTTACACGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATA TTTCTTCATG AAGACCTCAC AGTAAAAATA 
GGGGATTTTG GTCTGGCTAC AGTAAAATCT CGGTGGAGTG GCTCCCATCA GTTTGAACAG 
TTGTCTGGAT CTATCTTGTG GATGGCACCA GAGGTCATTA GAATGCAAGA TAAAAACCCA 
TATAGCTTTC AATCAGATGT ATATGCGTTT GGGATTGTTC TGTATGAGTT GATGACGGGA 
CAATTACCTT ACTCAAACAT CAACAACAGG GACCAGATAA TTTTTATGGT GGGGCGAGGA 
TACCTGTCTC CAGATCTCAG TAAGGTGCGG AGTAATTGTC CAAAAGCCAT GAAGAGGTTA 
ATGGCAGAGT GCCTGAAGAA GAAGAGAGAT GAGAGGCCAC TTTTTCCCCA GATCCTTGCC 
TCCATTGAAC TTCTGGCCCG CTCATTGCCA AAAATTCACC GCAGTGCATC TGAACCCTCA 
CTGAATCGGG CTGGCTTCCA AACGGAGGAT TTTAGTCTGT ATGCTTGTGC TTCTCCAAAA 
ACGCCCATCC AGGCAGGGGG ATATGGAGAA TTTGCCGCCT TCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020853807.1
CDS	239..2506
Translation	MIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESMGNGTDFSVSSSASTDTVTSSSSSSLSVAPSSLAVFQNPTDVSRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRRQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLGESTLTSGSSPSAPPSDSIGPPILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGLRGDGAPLNQLMRCLRKYQSRTPSPLLHSVPSEIVFDFEPGPVFRGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK

Target ORF information:

RefSeq Version	XM_020998148.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020998148.1

ORF Insert Sequence:

ATGATTAAAT TGACACAGGA ACACATAGAG GCCCTATTGG ACAAGTTCGG AGGGGAACAT 
AATCCACCAT CAATCTACCT GGAGGCCTAT GAAGAATATA CCAGCAAGCT AGATGCACTT 
CAACAACGGG AACAACAATT ATTAGAGTCC ATGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCAGCTT CAACAGACAC AGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGGCACCT 
TCATCTCTTG CAGTTTTTCA GAATCCTACA GATGTATCAA GAAGCAACCC TAAGTCACCA 
CAAAAACCAA TTGTTAGAGT CTTCCTGCCC AACAAACAGA GGACTGTGGT GCCAGCAAGA 
TGTGGGGTGA CAGTCCGAGA CAGTCTAAAG AAAGCATTGA TGATGAGAGG CCTGATCCCA 
GAATGCTGTG CTGTGTACAG AAGACAGGAT GGAGAGAAGA AGCCTATTGG CTGGGACACT 
GATATTTCCT GGCTTACTGG AGAAGAATTG CACGTAGAAG TTTTGGAGAA TGTTCCCCTA 
ACGACACACA ATTTTGTACG GAAGACATTT TTTACCTTAG CATTCTGTGA CTTTTGTCGA 
AAGTTGCTTT TCCAAGGTTT CCGCTGTCAG ACATGTGGTT ACAAATTTCA CCAACGCTGT 
AGTACAGAGG TGCCACTGAT GTGTGTTAAT TATGACCAGC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCGCAG GAGGAGGCCT CCTTAGGAGA GAGCACCCTT 
ACGTCGGGAT CATCCCCTTC AGCACCCCCC TCAGATTCTA TTGGGCCCCC AATTCTTACC 
AGTCCATCGC CTTCAAAATC CATTCCAATC CCACAGCCTT TTCGACCAGC AGATGAGGAT 
CATCGGAACC AGTTTGGGCA ACGAGACCGG TCCTCATCGG CTCCCAATGT GCATATAAAT 
ACAATAGAAC CTGTCAATAT TGATGACTTG ATTAGAGACC AAGGGTTACG GGGTGATGGA 
GCCCCCTTGA ACCAACTGAT GCGCTGTCTT CGGAAATACC AATCCCGGAC TCCCAGTCCC 
CTCCTACATT CTGTCCCCAG TGAAATAGTG TTTGATTTTG AGCCTGGCCC AGTGTTCAGA 
GGGTCAACCA CAGGCTTGTC TGCCACCCCT CCTGCTTCCT TGCCTGGATC TTTAACTAAT 
GTGAAAGCAT TACAAAAATC TCCAGGACCT CAGCGTGAAA GAAAATCTTC ATCATCTTCT 
GAAGACAGGA ATAGAATGAA AACCCTTGGT CGACGGGACT CAAGTGATGA TTGGGAGATT 
CCAGATGGGC AGATTACAGT TGGACAAAGG ATAGGATCTG GATCATTTGG AACAGTCTAC 
AAGGGAAAAT GGCATGGTGA TGTAGCTGTG AAAATGTTGA ATGTGACAGC ACCCACACCT 
CAGCAGTTAC AGGCTTTCAA AAATGAAGTA GGAGTGCTCA GGAAAACGCG ACATGTGAAT 
ATCCTACTCT TTATGGGCTA TTCAACAAAA CCACAGTTGG CCATTGTTAC ACAGTGGTGT 
GAAGGCTCCA GTTTATATCA CCATCTACAT ATCATTGAGA CCAAATTTGA GATGATCAAG 
CTAATAGATA TTGCACGGCA AACAGCACAA GGCATGGATT ACTTACACGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATA TTTCTTCATG AAGACCTCAC AGTAAAAATA 
GGGGATTTTG GTCTGGCTAC AGTAAAATCT CGGTGGAGTG GCTCCCATCA GTTTGAACAG 
TTGTCTGGAT CTATCTTGTG GATGGCACCA GAGGTCATTA GAATGCAAGA TAAAAACCCA 
TATAGCTTTC AATCAGATGT ATATGCGTTT GGGATTGTTC TGTATGAGTT GATGACGGGA 
CAATTACCTT ACTCAAACAT CAACAACAGG GACCAGATAA TTTTTATGGT GGGGCGAGGA 
TACCTGTCTC CAGATCTCAG TAAGGTGCGG AGTAATTGTC CAAAAGCCAT GAAGAGGTTA 
ATGGCAGAGT GCCTGAAGAA GAAGAGAGAT GAGAGGCCAC TTTTTCCCCA GATCCTTGCC 
TCCATTGAAC TTCTGGCCCG CTCATTGCCA AAAATTCACC GCAGTGCATC TGAACCCTCA 
CTGAATCGGG CTGGCTTCCA AACGGAGGAT TTTAGTCTGT ATGCTTGTGC TTCTCCAAAA 
ACGCCCATCC AGGCAGGGGG ATATGGAGAA TTTGCCGCCT TCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_020998149.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020853808.1 Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OPh263403
Clone ID Related Accession (Same CDS sequence)	XM_020998149.1
Accession Version	XM_020998149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	serine/threonine-protein kinase B-raf isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344022.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC TGAGCGGCGG CGGCGGCGGC GGCGCGGAGC AGGGCCCCGC TCTCTTCAAC 
GGGGACATGG ATCCCGAGGC TGGCGCCGGC TCGGCGTCCT CCGCCGAGCC GGCCATCCCC 
GAGGAGGTGT GGAACATCAA GCAAATGATT AAATTGACAC AGGAACACAT AGAGGCCCTA 
TTGGACAAGT TCGGAGGGGA ACATAATCCA CCATCAATCT ACCTGGAGGC CTATGAAGAA 
TATACCAGCA AGCTAGATGC ACTTCAACAA CGGGAACAAC AATTATTAGA GTCCATGGGG 
AATGGAACTG ATTTTTCTGT TTCTAGCTCA GCTTCAACAG ACACAGTTAC ATCTTCTTCC 
TCTTCTAGCC TTTCAGTGGC ACCTTCATCT CTTGCAGTTT TTCAGAATCC TACAGATGTA 
TCAAGAAGCA ACCCTAAGTC ACCACAAAAA CCAATTGTTA GAGTCTTCCT GCCCAACAAA 
CAGAGGACTG TGGTGCCAGC AAGATGTGGG GTGACAGTCC GAGACAGTCT AAAGAAAGCA 
TTGATGATGA GAGGCCTGAT CCCAGAATGC TGTGCTGTGT ACAGAAGACA GGATGGAGAG 
AAGAAGCCTA TTGGCTGGGA CACTGATATT TCCTGGCTTA CTGGAGAAGA ATTGCACGTA 
GAAGTTTTGG AGAATGTTCC CCTAACGACA CACAATTTTG TACGGAAGAC ATTTTTTACC 
TTAGCATTCT GTGACTTTTG TCGAAAGTTG CTTTTCCAAG GTTTCCGCTG TCAGACATGT 
GGTTACAAAT TTCACCAACG CTGTAGTACA GAGGTGCCAC TGATGTGTGT TAATTATGAC 
CAGCTTGATT TGCTGTTTGT CTCCAAGTTC TTTGAACACC ACCCAATACC GCAGGAGGAG 
GCCTCCTTAG GAGAGAGCAC CCTTACGTCG GGATCATCCC CTTCAGCACC CCCCTCAGAT 
TCTATTGGGC CCCCAATTCT TACCAGTCCA TCGCCTTCAA AATCCATTCC AATCCCACAG 
CCTTTTCGAC CAGCAGATGA GGATCATCGG AACCAGTTTG GGCAACGAGA CCGGTCCTCA 
TCGGCTCCCA ATGTGCATAT AAATACAATA GAACCTGTCA ATATTGATGA CTTGATTAGA 
GACCAAGGGT TACGGGGTGA TGGAGCCCCC TTGAACCAAC TGATGCGCTG TCTTCGGAAA 
TACCAATCCC GGACTCCCAG TCCCCTCCTA CATTCTGTCC CCAGTGAAAT AGTGTTTGAT 
TTTGAGCCTG GCCCAGTGTT CAGAGGGTCA ACCACAGGCT TGTCTGCCAC CCCTCCTGCT 
TCCTTGCCTG GATCTTTAAC TAATGTGAAA GCATTACAAA AATCTCCAGG ACCTCAGCGT 
GAAAGAAAAT CTTCATCATC TTCTGAAGAC AGGAATAGAA TGAAAACCCT TGGTCGACGG 
GACTCAAGTG ATGATTGGGA GATTCCAGAT GGGCAGATTA CAGTTGGACA AAGGATAGGA 
TCTGGATCAT TTGGAACAGT CTACAAGGGA AAATGGCATG GTGATGTAGC TGTGAAAATG 
TTGAATGTGA CAGCACCCAC ACCTCAGCAG TTACAGGCTT TCAAAAATGA AGTAGGAGTG 
CTCAGGAAAA CGCGACATGT GAATATCCTA CTCTTTATGG GCTATTCAAC AAAACCACAG 
TTGGCCATTG TTACACAGTG GTGTGAAGGC TCCAGTTTAT ATCACCATCT ACATATCATT 
GAGACCAAAT TTGAGATGAT CAAGCTAATA GATATTGCAC GGCAAACAGC ACAAGGCATG 
GATTACTTAC ACGCCAAGTC AATCATCCAC AGAGACCTCA AGAGTAATAA TATATTTCTT 
CATGAAGACC TCACAGTAAA AATAGGGGAT TTTGGTCTGG CTACAGTAAA ATCTCGGTGG 
AGTGGCTCCC ATCAGTTTGA ACAGTTGTCT GGATCTATCT TGTGGATGGC ACCAGAGGTC 
ATTAGAATGC AAGATAAAAA CCCATATAGC TTTCAATCAG ATGTATATGC GTTTGGGATT 
GTTCTGTATG AGTTGATGAC GGGACAATTA CCTTACTCAA ACATCAACAA CAGGGACCAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020853808.1
CDS	48..2153
Translation	MAALSGGGGGGAEQGPALFNGDMDPEAGAGSASSAEPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESMGNGTDFSVSSSASTDTVTSSSSSSLSVAPSSLAVFQNPTDVSRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRRQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLGESTLTSGSSPSAPPSDSIGPPILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGLRGDGAPLNQLMRCLRKYQSRTPSPLLHSVPSEIVFDFEPGPVFRGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQE

Target ORF information:

RefSeq Version	XM_020998149.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020998149.1

ORF Insert Sequence:

ATGGCGGCGC TGAGCGGCGG CGGCGGCGGC GGCGCGGAGC AGGGCCCCGC TCTCTTCAAC 
GGGGACATGG ATCCCGAGGC TGGCGCCGGC TCGGCGTCCT CCGCCGAGCC GGCCATCCCC 
GAGGAGGTGT GGAACATCAA GCAAATGATT AAATTGACAC AGGAACACAT AGAGGCCCTA 
TTGGACAAGT TCGGAGGGGA ACATAATCCA CCATCAATCT ACCTGGAGGC CTATGAAGAA 
TATACCAGCA AGCTAGATGC ACTTCAACAA CGGGAACAAC AATTATTAGA GTCCATGGGG 
AATGGAACTG ATTTTTCTGT TTCTAGCTCA GCTTCAACAG ACACAGTTAC ATCTTCTTCC 
TCTTCTAGCC TTTCAGTGGC ACCTTCATCT CTTGCAGTTT TTCAGAATCC TACAGATGTA 
TCAAGAAGCA ACCCTAAGTC ACCACAAAAA CCAATTGTTA GAGTCTTCCT GCCCAACAAA 
CAGAGGACTG TGGTGCCAGC AAGATGTGGG GTGACAGTCC GAGACAGTCT AAAGAAAGCA 
TTGATGATGA GAGGCCTGAT CCCAGAATGC TGTGCTGTGT ACAGAAGACA GGATGGAGAG 
AAGAAGCCTA TTGGCTGGGA CACTGATATT TCCTGGCTTA CTGGAGAAGA ATTGCACGTA 
GAAGTTTTGG AGAATGTTCC CCTAACGACA CACAATTTTG TACGGAAGAC ATTTTTTACC 
TTAGCATTCT GTGACTTTTG TCGAAAGTTG CTTTTCCAAG GTTTCCGCTG TCAGACATGT 
GGTTACAAAT TTCACCAACG CTGTAGTACA GAGGTGCCAC TGATGTGTGT TAATTATGAC 
CAGCTTGATT TGCTGTTTGT CTCCAAGTTC TTTGAACACC ACCCAATACC GCAGGAGGAG 
GCCTCCTTAG GAGAGAGCAC CCTTACGTCG GGATCATCCC CTTCAGCACC CCCCTCAGAT 
TCTATTGGGC CCCCAATTCT TACCAGTCCA TCGCCTTCAA AATCCATTCC AATCCCACAG 
CCTTTTCGAC CAGCAGATGA GGATCATCGG AACCAGTTTG GGCAACGAGA CCGGTCCTCA 
TCGGCTCCCA ATGTGCATAT AAATACAATA GAACCTGTCA ATATTGATGA CTTGATTAGA 
GACCAAGGGT TACGGGGTGA TGGAGCCCCC TTGAACCAAC TGATGCGCTG TCTTCGGAAA 
TACCAATCCC GGACTCCCAG TCCCCTCCTA CATTCTGTCC CCAGTGAAAT AGTGTTTGAT 
TTTGAGCCTG GCCCAGTGTT CAGAGGGTCA ACCACAGGCT TGTCTGCCAC CCCTCCTGCT 
TCCTTGCCTG GATCTTTAAC TAATGTGAAA GCATTACAAA AATCTCCAGG ACCTCAGCGT 
GAAAGAAAAT CTTCATCATC TTCTGAAGAC AGGAATAGAA TGAAAACCCT TGGTCGACGG 
GACTCAAGTG ATGATTGGGA GATTCCAGAT GGGCAGATTA CAGTTGGACA AAGGATAGGA 
TCTGGATCAT TTGGAACAGT CTACAAGGGA AAATGGCATG GTGATGTAGC TGTGAAAATG 
TTGAATGTGA CAGCACCCAC ACCTCAGCAG TTACAGGCTT TCAAAAATGA AGTAGGAGTG 
CTCAGGAAAA CGCGACATGT GAATATCCTA CTCTTTATGG GCTATTCAAC AAAACCACAG 
TTGGCCATTG TTACACAGTG GTGTGAAGGC TCCAGTTTAT ATCACCATCT ACATATCATT 
GAGACCAAAT TTGAGATGAT CAAGCTAATA GATATTGCAC GGCAAACAGC ACAAGGCATG 
GATTACTTAC ACGCCAAGTC AATCATCCAC AGAGACCTCA AGAGTAATAA TATATTTCTT 
CATGAAGACC TCACAGTAAA AATAGGGGAT TTTGGTCTGG CTACAGTAAA ATCTCGGTGG 
AGTGGCTCCC ATCAGTTTGA ACAGTTGTCT GGATCTATCT TGTGGATGGC ACCAGAGGTC 
ATTAGAATGC AAGATAAAAA CCCATATAGC TTTCAATCAG ATGTATATGC GTTTGGGATT 
GTTCTGTATG AGTTGATGAC GGGACAATTA CCTTACTCAA ACATCAACAA CAGGGACCAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

BRAF cDNA ORF clone, Phascolarctos cinereus(koala)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム