USPL1 cDNA ORF clone, Phascolarctos cinereus(koala)

Gene
Clones
gRNAs

Summary

Gene Symbol	USPL1
Entrez Gene ID	110215543
Full Name	ubiquitin specific peptidase like 1
Gene Type	protein-coding
Organism	Phascolarctos cinereus(koala)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020997127.1	XP_020852786.1	SUMO-specific isopeptidase USPL1
XM_020997128.1	XP_020852787.1	SUMO-specific isopeptidase USPL1

Pathways from BioSystems
Gene Ontology

The following USPL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USPL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPh262637	XM_020997127.1 Latest version!	Phascolarctos cinereus ubiquitin specific peptidase like 1 (USPL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPh262637	XM_020997128.1 Latest version!	Phascolarctos cinereus ubiquitin specific peptidase like 1 (USPL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USPL1 ( XM_020997127.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020852786.1 Phascolarctos cinereus ubiquitin specific peptidase like 1 (USPL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPh262637
Clone ID Related Accession (Same CDS sequence)	XM_020997127.1 , XM_020997128.1
Accession Version	XM_020997127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	SUMO-specific isopeptidase USPL1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344018.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATT CCCAGAAGAT TGGAAATGGT TTGCCAGTGA TTGGACAAGA CACTGGTATA 
GGGATATCTT CACTCCACAT GGTGGGGTAT TTGGGAAAAA AGTGTGATTC AGCTAAAGTT 
ACGTCAGATG GGTACTGTCC AGCCTGTAGA GAGAAGGGAA AGATCAAAAC TCTAAAGTCT 
TATCGAATCA GTTTTCAAGA ATCTATCTCT TTGTGTGAAG ATCCACAGTG CATCTATCCA 
TTGGGCTATA AGTCATTAGA TAACATTATA GTCTCTGTTG ATTTGGAAAA TTATCAGCCT 
CCCCGTAAAC AGCTAAAGAG GAAGATCTTG GAAAGCAGCA GTGTTGCTTC ATCTTTGGAA 
CCAAATTCAA AGGAAGATGG CAGCAACACA TGGTTCGACA GTGAATGTAC TGTGAACAGT 
GAGCTTGCTC ACAGACCTGA TGGATATAAT TTGTATAGAA CTTCATCAGA TTTATCTGTT 
GTGTTACAAG ACTGGCAACA GAACCCAAGC AAGGCAGCTG AATCTTTGCA GCATAGTGGG 
ATTTTGGAAG CTGAAATTGC AGACCAGACA GCTGAAGAAC ATCATTTGAT TGTTGGTTCT 
CAGACTGAGA GTATTCCAGA GCCTGAAGTG GATGCAACTA AATTAGAAAT CCTACTGGAG 
AATCGTTGCC TGCCATTTAC TGAATCTCCA TGTCTTCAGT GGAGAAATGC ATATGCTCTC 
TGTTGGTTAG ACTGTATTCT CTCAGCATTA GTGCACTTGG AAGGGTTAAA GAAAGCTGTG 
ACTGAACTAT GTCCTAAAGA AGAATCTATG TTCCAGCAGT TGTATATAAA GTATAATCAA 
GCGATCCTGC TTTTGACTGC TCATCATTTG AATGGAGCTA AAGATGAAAG TTCTATAAGG 
AATGTTTCAG ATAGACTTCG AAAGGCTGAG ACTCACCTAA ATGAAGTCAG AAACAAAATT 
TTTGCTAAGC TTCAACCAAA ACTTCATTGT AAGTTAGGTA ATATGGAAAG TCCTGTGTTT 
GCATTTCCCT TGCTTCTACA AATGGAACCT CTCATTGAAA CGCTCTTCAT GTCATCTTTT 
TCTTGGAACT TTGAGTGTTC TCAGTGTGGA AACAAATATC AACAGAGGTG TTCAAAGAAT 
CTGGTAACCT TCACAAACGT AATACCTGAG TGGCATCCAC TTGATGCTGT CCATTTTGGT 
CCATGTAACA GTTGTAAAAG TAAATTACAA AGAAGAAGAA TGGTATTGGA AAAAGTATCT 
TCAGTATTTA TGCTGCACTT TGTAGAAGGC TTGCCCTGTA ATGACTTGCA GTTCTATTCA 
TTTCATTTTG AAGGCTTTTT CTACCAAATA ACATGTGTGA TTCAATATCA AATGAATGAA 
AAACATTTTA TAAGTTGGAT TTTGAATTCT GATGGAACTT GGCTAGAATG TGATGATTTA 
AAAGGACCAT ACTGTAAAAG ACATCAGAGA TTTGAAGTTC CAGCTTCAGA GATTCACATT 
GTTATCTGGG AAAGGAACAT TTCCAAAATA ATAGATCAAT CCAATATAGA ATTTCCAGTT 
AAAAAGACAG AAAGTTTCAG CCTCAGTGAT TCTAAACCAG AATCTCTGGC ATTGTGTTCT 
GAGGGGGATG CTGCCACTGA AATGTTTTCA GCGTTTAATC AGGAGAATAT TCTAAGTGCT 
CCAACTACTG ATGCACAATA TGTAGTAGCC ACAGATCATG AGTACAGTTT ACTTTCTGGT 
CTAGAAGATT TGGTAGATAA TGATATTATA ACTTTAACAC TCACAGAAAT TCATGTTGAT 
TCTGAGGGCA ATCCAATTGA AAATCAGCCA GTGATAGAAG ATCAGTCTGC GATAAGAGAT 
AAGGCAGTTG CTGAAATGGA CACTTTGCAA AAGCAAGAAT TAGTGTTAGC TTCTTCACAA 
TCAGCTCCAT GTAGGGAAAC ACCTGTCCAA CCTCAAACAC TGCCTACTAG TATCCTAACT 
CAGAATGCTA GTATTGATTT GAAATTGGAT CAGCTGAATA CAGAAAATAC TTCAGATACT 
ATATGCGTGA ATAACACAGT ACATGTGTCT ACTCTTCCGC AAGGAGAGAA GCCATTACAA 
ATTCAAGAGA CAAAAGTTAA TTTTCCTCCC ATTGCCTTGG AGAAGGGTGC TATGTCAAAT 
CCAGTCCTTT CATCAAAGGT TGAAAAACTT ATGTCAAAAC AAAGTGTCTT ATCTCTAGGA 
TCTAATTTGA AAACAGCAAA TAATATAGGA GATTCCCAAA GTGTGGTCCT GGGTTCAACA 
CATAATTCTT CACAGTCCCA GTCACAGAAA AAAAAGTTTG TAGGAAGTTG GGTAAAAGGA 
TTACTGAACA AGGGAGTTTC TTTTATGCCA TCTTGTGCCT CAGCTAATGA AAGAAAGGTC 
CATTTGCAAA AGACAACTGA CTTGAACTCT CTGGTTAAAA GGGCAAATAA TTTTCGGGGC 
TTTAAACCTA AAGGTATAAC AAATCAAACA AGTCCTCAAG CACCCAAGAA AACATCCAGG 
TCTGCATGCA ACTCACTTCC AGTTCTTAAC TCTTCACCAT CAAATATCAG CACATCCTTA 
ATACCTGACG AAGTTCGGGA GGGTACAAAG ATTTTGACTA AATCTTTGCA TAAAAATGTT 
CAGCCCCTCT CTAGTGGGAT ACCTCCTAAG CACAATTTTA ATGGAAATGA AAATCACATC 
TCTTCAGGGA CGTATGAGAG TCAGACTCAT AAACTTCGTC TAAAACTTCT TAAAAAACTT 
AAGGCAAAAA AGCAAAAACT GGCCACTCTT CAGGCTCTCC AAATAAATGG AAAGCCTCCA 
AAGAAAAGTA CAAAACCCTT GCCCCAGGAT GCATCTCCAA ATGAATGTGA ATCACTGCAA 
GGCCTGTTAA TGGAACTCCA GGATCATATC AGTGCCACAG ACAATAAACC TAAAGGTACC 
ACACATTCCA ATATTTCCCT GTGTAATAGC CCAACCCACG AAGAATTTTT AGCAGAATTA 
TTGTCGTCTA CTACAACTGT CGCCTCATCA GAACTACCAA TGAATAGAGA AAATGATTGT 
GAGTATTTGG AGATGGTAGA TAACCATAAC ATGACACCAA CACCTAGTGA CTTTGTCAAT 
GGATTCTCTT CTGCATATCC TAGCAAAGAC CATAATTATT ATAGTCCTGT GAAACAAAGT 
CAGTATGAAG TTCAGATGGA CTCATTAACA AATAATTCTT GTGCTAAAAC GTTAAACTTG 
GAAAATCCCC TAAAGACAGA CATTCTTGAT GAATTTTTTT CCACTTCGGT ATTAAGTTCT 
TCAGCAGATG ACATAGAATA TTTACCTCAT TTTGATGAAT ATATGTTTGA CAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020852786.1
CDS	209..3565
Translation	MMDSQKIGNGLPVIGQDTGIGISSLHMVGYLGKKCDSAKVTSDGYCPACREKGKIKTLKSYRISFQESISLCEDPQCIYPLGYKSLDNIIVSVDLENYQPPRKQLKRKILESSSVASSLEPNSKEDGSNTWFDSECTVNSELAHRPDGYNLYRTSSDLSVVLQDWQQNPSKAAESLQHSGILEAEIADQTAEEHHLIVGSQTESIPEPEVDATKLEILLENRCLPFTESPCLQWRNAYALCWLDCILSALVHLEGLKKAVTELCPKEESMFQQLYIKYNQAILLLTAHHLNGAKDESSIRNVSDRLRKAETHLNEVRNKIFAKLQPKLHCKLGNMESPVFAFPLLLQMEPLIETLFMSSFSWNFECSQCGNKYQQRCSKNLVTFTNVIPEWHPLDAVHFGPCNSCKSKLQRRRMVLEKVSSVFMLHFVEGLPCNDLQFYSFHFEGFFYQITCVIQYQMNEKHFISWILNSDGTWLECDDLKGPYCKRHQRFEVPASEIHIVIWERNISKIIDQSNIEFPVKKTESFSLSDSKPESLALCSEGDAATEMFSAFNQENILSAPTTDAQYVVATDHEYSLLSGLEDLVDNDIITLTLTEIHVDSEGNPIENQPVIEDQSAIRDKAVAEMDTLQKQELVLASSQSAPCRETPVQPQTLPTSILTQNASIDLKLDQLNTENTSDTICVNNTVHVSTLPQGEKPLQIQETKVNFPPIALEKGAMSNPVLSSKVEKLMSKQSVLSLGSNLKTANNIGDSQSVVLGSTHNSSQSQSQKKKFVGSWVKGLLNKGVSFMPSCASANERKVHLQKTTDLNSLVKRANNFRGFKPKGITNQTSPQAPKKTSRSACNSLPVLNSSPSNISTSLIPDEVREGTKILTKSLHKNVQPLSSGIPPKHNFNGNENHISSGTYESQTHKLRLKLLKKLKAKKQKLATLQALQINGKPPKKSTKPLPQDASPNECESLQGLLMELQDHISATDNKPKGTTHSNISLCNSPTHEEFLAELLSSTTTVASSELPMNRENDCEYLEMVDNHNMTPTPSDFVNGFSSAYPSKDHNYYSPVKQSQYEVQMDSLTNNSCAKTLNLENPLKTDILDEFFSTSVLSSSADDIEYLPHFDEYMFDS

Target ORF information:

RefSeq Version	XM_020997127.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus ubiquitin specific peptidase like 1 (USPL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020997127.1

ORF Insert Sequence:

ATGATGGATT CCCAGAAGAT TGGAAATGGT TTGCCAGTGA TTGGACAAGA CACTGGTATA 
GGGATATCTT CACTCCACAT GGTGGGGTAT TTGGGAAAAA AGTGTGATTC AGCTAAAGTT 
ACGTCAGATG GGTACTGTCC AGCCTGTAGA GAGAAGGGAA AGATCAAAAC TCTAAAGTCT 
TATCGAATCA GTTTTCAAGA ATCTATCTCT TTGTGTGAAG ATCCACAGTG CATCTATCCA 
TTGGGCTATA AGTCATTAGA TAACATTATA GTCTCTGTTG ATTTGGAAAA TTATCAGCCT 
CCCCGTAAAC AGCTAAAGAG GAAGATCTTG GAAAGCAGCA GTGTTGCTTC ATCTTTGGAA 
CCAAATTCAA AGGAAGATGG CAGCAACACA TGGTTCGACA GTGAATGTAC TGTGAACAGT 
GAGCTTGCTC ACAGACCTGA TGGATATAAT TTGTATAGAA CTTCATCAGA TTTATCTGTT 
GTGTTACAAG ACTGGCAACA GAACCCAAGC AAGGCAGCTG AATCTTTGCA GCATAGTGGG 
ATTTTGGAAG CTGAAATTGC AGACCAGACA GCTGAAGAAC ATCATTTGAT TGTTGGTTCT 
CAGACTGAGA GTATTCCAGA GCCTGAAGTG GATGCAACTA AATTAGAAAT CCTACTGGAG 
AATCGTTGCC TGCCATTTAC TGAATCTCCA TGTCTTCAGT GGAGAAATGC ATATGCTCTC 
TGTTGGTTAG ACTGTATTCT CTCAGCATTA GTGCACTTGG AAGGGTTAAA GAAAGCTGTG 
ACTGAACTAT GTCCTAAAGA AGAATCTATG TTCCAGCAGT TGTATATAAA GTATAATCAA 
GCGATCCTGC TTTTGACTGC TCATCATTTG AATGGAGCTA AAGATGAAAG TTCTATAAGG 
AATGTTTCAG ATAGACTTCG AAAGGCTGAG ACTCACCTAA ATGAAGTCAG AAACAAAATT 
TTTGCTAAGC TTCAACCAAA ACTTCATTGT AAGTTAGGTA ATATGGAAAG TCCTGTGTTT 
GCATTTCCCT TGCTTCTACA AATGGAACCT CTCATTGAAA CGCTCTTCAT GTCATCTTTT 
TCTTGGAACT TTGAGTGTTC TCAGTGTGGA AACAAATATC AACAGAGGTG TTCAAAGAAT 
CTGGTAACCT TCACAAACGT AATACCTGAG TGGCATCCAC TTGATGCTGT CCATTTTGGT 
CCATGTAACA GTTGTAAAAG TAAATTACAA AGAAGAAGAA TGGTATTGGA AAAAGTATCT 
TCAGTATTTA TGCTGCACTT TGTAGAAGGC TTGCCCTGTA ATGACTTGCA GTTCTATTCA 
TTTCATTTTG AAGGCTTTTT CTACCAAATA ACATGTGTGA TTCAATATCA AATGAATGAA 
AAACATTTTA TAAGTTGGAT TTTGAATTCT GATGGAACTT GGCTAGAATG TGATGATTTA 
AAAGGACCAT ACTGTAAAAG ACATCAGAGA TTTGAAGTTC CAGCTTCAGA GATTCACATT 
GTTATCTGGG AAAGGAACAT TTCCAAAATA ATAGATCAAT CCAATATAGA ATTTCCAGTT 
AAAAAGACAG AAAGTTTCAG CCTCAGTGAT TCTAAACCAG AATCTCTGGC ATTGTGTTCT 
GAGGGGGATG CTGCCACTGA AATGTTTTCA GCGTTTAATC AGGAGAATAT TCTAAGTGCT 
CCAACTACTG ATGCACAATA TGTAGTAGCC ACAGATCATG AGTACAGTTT ACTTTCTGGT 
CTAGAAGATT TGGTAGATAA TGATATTATA ACTTTAACAC TCACAGAAAT TCATGTTGAT 
TCTGAGGGCA ATCCAATTGA AAATCAGCCA GTGATAGAAG ATCAGTCTGC GATAAGAGAT 
AAGGCAGTTG CTGAAATGGA CACTTTGCAA AAGCAAGAAT TAGTGTTAGC TTCTTCACAA 
TCAGCTCCAT GTAGGGAAAC ACCTGTCCAA CCTCAAACAC TGCCTACTAG TATCCTAACT 
CAGAATGCTA GTATTGATTT GAAATTGGAT CAGCTGAATA CAGAAAATAC TTCAGATACT 
ATATGCGTGA ATAACACAGT ACATGTGTCT ACTCTTCCGC AAGGAGAGAA GCCATTACAA 
ATTCAAGAGA CAAAAGTTAA TTTTCCTCCC ATTGCCTTGG AGAAGGGTGC TATGTCAAAT 
CCAGTCCTTT CATCAAAGGT TGAAAAACTT ATGTCAAAAC AAAGTGTCTT ATCTCTAGGA 
TCTAATTTGA AAACAGCAAA TAATATAGGA GATTCCCAAA GTGTGGTCCT GGGTTCAACA 
CATAATTCTT CACAGTCCCA GTCACAGAAA AAAAAGTTTG TAGGAAGTTG GGTAAAAGGA 
TTACTGAACA AGGGAGTTTC TTTTATGCCA TCTTGTGCCT CAGCTAATGA AAGAAAGGTC 
CATTTGCAAA AGACAACTGA CTTGAACTCT CTGGTTAAAA GGGCAAATAA TTTTCGGGGC 
TTTAAACCTA AAGGTATAAC AAATCAAACA AGTCCTCAAG CACCCAAGAA AACATCCAGG 
TCTGCATGCA ACTCACTTCC AGTTCTTAAC TCTTCACCAT CAAATATCAG CACATCCTTA 
ATACCTGACG AAGTTCGGGA GGGTACAAAG ATTTTGACTA AATCTTTGCA TAAAAATGTT 
CAGCCCCTCT CTAGTGGGAT ACCTCCTAAG CACAATTTTA ATGGAAATGA AAATCACATC 
TCTTCAGGGA CGTATGAGAG TCAGACTCAT AAACTTCGTC TAAAACTTCT TAAAAAACTT 
AAGGCAAAAA AGCAAAAACT GGCCACTCTT CAGGCTCTCC AAATAAATGG AAAGCCTCCA 
AAGAAAAGTA CAAAACCCTT GCCCCAGGAT GCATCTCCAA ATGAATGTGA ATCACTGCAA 
GGCCTGTTAA TGGAACTCCA GGATCATATC AGTGCCACAG ACAATAAACC TAAAGGTACC 
ACACATTCCA ATATTTCCCT GTGTAATAGC CCAACCCACG AAGAATTTTT AGCAGAATTA 
TTGTCGTCTA CTACAACTGT CGCCTCATCA GAACTACCAA TGAATAGAGA AAATGATTGT 
GAGTATTTGG AGATGGTAGA TAACCATAAC ATGACACCAA CACCTAGTGA CTTTGTCAAT 
GGATTCTCTT CTGCATATCC TAGCAAAGAC CATAATTATT ATAGTCCTGT GAAACAAAGT 
CAGTATGAAG TTCAGATGGA CTCATTAACA AATAATTCTT GTGCTAAAAC GTTAAACTTG 
GAAAATCCCC TAAAGACAGA CATTCTTGAT GAATTTTTTT CCACTTCGGT ATTAAGTTCT 
TCAGCAGATG ACATAGAATA TTTACCTCAT TTTGATGAAT ATATGTTTGA CAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USPL1 ( XM_020997128.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020852787.1 Phascolarctos cinereus ubiquitin specific peptidase like 1 (USPL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPh262637
Clone ID Related Accession (Same CDS sequence)	XM_020997127.1 , XM_020997128.1
Accession Version	XM_020997128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	SUMO-specific isopeptidase USPL1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344018.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATT CCCAGAAGAT TGGAAATGGT TTGCCAGTGA TTGGACAAGA CACTGGTATA 
GGGATATCTT CACTCCACAT GGTGGGGTAT TTGGGAAAAA AGTGTGATTC AGCTAAAGTT 
ACGTCAGATG GGTACTGTCC AGCCTGTAGA GAGAAGGGAA AGATCAAAAC TCTAAAGTCT 
TATCGAATCA GTTTTCAAGA ATCTATCTCT TTGTGTGAAG ATCCACAGTG CATCTATCCA 
TTGGGCTATA AGTCATTAGA TAACATTATA GTCTCTGTTG ATTTGGAAAA TTATCAGCCT 
CCCCGTAAAC AGCTAAAGAG GAAGATCTTG GAAAGCAGCA GTGTTGCTTC ATCTTTGGAA 
CCAAATTCAA AGGAAGATGG CAGCAACACA TGGTTCGACA GTGAATGTAC TGTGAACAGT 
GAGCTTGCTC ACAGACCTGA TGGATATAAT TTGTATAGAA CTTCATCAGA TTTATCTGTT 
GTGTTACAAG ACTGGCAACA GAACCCAAGC AAGGCAGCTG AATCTTTGCA GCATAGTGGG 
ATTTTGGAAG CTGAAATTGC AGACCAGACA GCTGAAGAAC ATCATTTGAT TGTTGGTTCT 
CAGACTGAGA GTATTCCAGA GCCTGAAGTG GATGCAACTA AATTAGAAAT CCTACTGGAG 
AATCGTTGCC TGCCATTTAC TGAATCTCCA TGTCTTCAGT GGAGAAATGC ATATGCTCTC 
TGTTGGTTAG ACTGTATTCT CTCAGCATTA GTGCACTTGG AAGGGTTAAA GAAAGCTGTG 
ACTGAACTAT GTCCTAAAGA AGAATCTATG TTCCAGCAGT TGTATATAAA GTATAATCAA 
GCGATCCTGC TTTTGACTGC TCATCATTTG AATGGAGCTA AAGATGAAAG TTCTATAAGG 
AATGTTTCAG ATAGACTTCG AAAGGCTGAG ACTCACCTAA ATGAAGTCAG AAACAAAATT 
TTTGCTAAGC TTCAACCAAA ACTTCATTGT AAGTTAGGTA ATATGGAAAG TCCTGTGTTT 
GCATTTCCCT TGCTTCTACA AATGGAACCT CTCATTGAAA CGCTCTTCAT GTCATCTTTT 
TCTTGGAACT TTGAGTGTTC TCAGTGTGGA AACAAATATC AACAGAGGTG TTCAAAGAAT 
CTGGTAACCT TCACAAACGT AATACCTGAG TGGCATCCAC TTGATGCTGT CCATTTTGGT 
CCATGTAACA GTTGTAAAAG TAAATTACAA AGAAGAAGAA TGGTATTGGA AAAAGTATCT 
TCAGTATTTA TGCTGCACTT TGTAGAAGGC TTGCCCTGTA ATGACTTGCA GTTCTATTCA 
TTTCATTTTG AAGGCTTTTT CTACCAAATA ACATGTGTGA TTCAATATCA AATGAATGAA 
AAACATTTTA TAAGTTGGAT TTTGAATTCT GATGGAACTT GGCTAGAATG TGATGATTTA 
AAAGGACCAT ACTGTAAAAG ACATCAGAGA TTTGAAGTTC CAGCTTCAGA GATTCACATT 
GTTATCTGGG AAAGGAACAT TTCCAAAATA ATAGATCAAT CCAATATAGA ATTTCCAGTT 
AAAAAGACAG AAAGTTTCAG CCTCAGTGAT TCTAAACCAG AATCTCTGGC ATTGTGTTCT 
GAGGGGGATG CTGCCACTGA AATGTTTTCA GCGTTTAATC AGGAGAATAT TCTAAGTGCT 
CCAACTACTG ATGCACAATA TGTAGTAGCC ACAGATCATG AGTACAGTTT ACTTTCTGGT 
CTAGAAGATT TGGTAGATAA TGATATTATA ACTTTAACAC TCACAGAAAT TCATGTTGAT 
TCTGAGGGCA ATCCAATTGA AAATCAGCCA GTGATAGAAG ATCAGTCTGC GATAAGAGAT 
AAGGCAGTTG CTGAAATGGA CACTTTGCAA AAGCAAGAAT TAGTGTTAGC TTCTTCACAA 
TCAGCTCCAT GTAGGGAAAC ACCTGTCCAA CCTCAAACAC TGCCTACTAG TATCCTAACT 
CAGAATGCTA GTATTGATTT GAAATTGGAT CAGCTGAATA CAGAAAATAC TTCAGATACT 
ATATGCGTGA ATAACACAGT ACATGTGTCT ACTCTTCCGC AAGGAGAGAA GCCATTACAA 
ATTCAAGAGA CAAAAGTTAA TTTTCCTCCC ATTGCCTTGG AGAAGGGTGC TATGTCAAAT 
CCAGTCCTTT CATCAAAGGT TGAAAAACTT ATGTCAAAAC AAAGTGTCTT ATCTCTAGGA 
TCTAATTTGA AAACAGCAAA TAATATAGGA GATTCCCAAA GTGTGGTCCT GGGTTCAACA 
CATAATTCTT CACAGTCCCA GTCACAGAAA AAAAAGTTTG TAGGAAGTTG GGTAAAAGGA 
TTACTGAACA AGGGAGTTTC TTTTATGCCA TCTTGTGCCT CAGCTAATGA AAGAAAGGTC 
CATTTGCAAA AGACAACTGA CTTGAACTCT CTGGTTAAAA GGGCAAATAA TTTTCGGGGC 
TTTAAACCTA AAGGTATAAC AAATCAAACA AGTCCTCAAG CACCCAAGAA AACATCCAGG 
TCTGCATGCA ACTCACTTCC AGTTCTTAAC TCTTCACCAT CAAATATCAG CACATCCTTA 
ATACCTGACG AAGTTCGGGA GGGTACAAAG ATTTTGACTA AATCTTTGCA TAAAAATGTT 
CAGCCCCTCT CTAGTGGGAT ACCTCCTAAG CACAATTTTA ATGGAAATGA AAATCACATC 
TCTTCAGGGA CGTATGAGAG TCAGACTCAT AAACTTCGTC TAAAACTTCT TAAAAAACTT 
AAGGCAAAAA AGCAAAAACT GGCCACTCTT CAGGCTCTCC AAATAAATGG AAAGCCTCCA 
AAGAAAAGTA CAAAACCCTT GCCCCAGGAT GCATCTCCAA ATGAATGTGA ATCACTGCAA 
GGCCTGTTAA TGGAACTCCA GGATCATATC AGTGCCACAG ACAATAAACC TAAAGGTACC 
ACACATTCCA ATATTTCCCT GTGTAATAGC CCAACCCACG AAGAATTTTT AGCAGAATTA 
TTGTCGTCTA CTACAACTGT CGCCTCATCA GAACTACCAA TGAATAGAGA AAATGATTGT 
GAGTATTTGG AGATGGTAGA TAACCATAAC ATGACACCAA CACCTAGTGA CTTTGTCAAT 
GGATTCTCTT CTGCATATCC TAGCAAAGAC CATAATTATT ATAGTCCTGT GAAACAAAGT 
CAGTATGAAG TTCAGATGGA CTCATTAACA AATAATTCTT GTGCTAAAAC GTTAAACTTG 
GAAAATCCCC TAAAGACAGA CATTCTTGAT GAATTTTTTT CCACTTCGGT ATTAAGTTCT 
TCAGCAGATG ACATAGAATA TTTACCTCAT TTTGATGAAT ATATGTTTGA CAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020852787.1
CDS	1842..5198
Translation	MMDSQKIGNGLPVIGQDTGIGISSLHMVGYLGKKCDSAKVTSDGYCPACREKGKIKTLKSYRISFQESISLCEDPQCIYPLGYKSLDNIIVSVDLENYQPPRKQLKRKILESSSVASSLEPNSKEDGSNTWFDSECTVNSELAHRPDGYNLYRTSSDLSVVLQDWQQNPSKAAESLQHSGILEAEIADQTAEEHHLIVGSQTESIPEPEVDATKLEILLENRCLPFTESPCLQWRNAYALCWLDCILSALVHLEGLKKAVTELCPKEESMFQQLYIKYNQAILLLTAHHLNGAKDESSIRNVSDRLRKAETHLNEVRNKIFAKLQPKLHCKLGNMESPVFAFPLLLQMEPLIETLFMSSFSWNFECSQCGNKYQQRCSKNLVTFTNVIPEWHPLDAVHFGPCNSCKSKLQRRRMVLEKVSSVFMLHFVEGLPCNDLQFYSFHFEGFFYQITCVIQYQMNEKHFISWILNSDGTWLECDDLKGPYCKRHQRFEVPASEIHIVIWERNISKIIDQSNIEFPVKKTESFSLSDSKPESLALCSEGDAATEMFSAFNQENILSAPTTDAQYVVATDHEYSLLSGLEDLVDNDIITLTLTEIHVDSEGNPIENQPVIEDQSAIRDKAVAEMDTLQKQELVLASSQSAPCRETPVQPQTLPTSILTQNASIDLKLDQLNTENTSDTICVNNTVHVSTLPQGEKPLQIQETKVNFPPIALEKGAMSNPVLSSKVEKLMSKQSVLSLGSNLKTANNIGDSQSVVLGSTHNSSQSQSQKKKFVGSWVKGLLNKGVSFMPSCASANERKVHLQKTTDLNSLVKRANNFRGFKPKGITNQTSPQAPKKTSRSACNSLPVLNSSPSNISTSLIPDEVREGTKILTKSLHKNVQPLSSGIPPKHNFNGNENHISSGTYESQTHKLRLKLLKKLKAKKQKLATLQALQINGKPPKKSTKPLPQDASPNECESLQGLLMELQDHISATDNKPKGTTHSNISLCNSPTHEEFLAELLSSTTTVASSELPMNRENDCEYLEMVDNHNMTPTPSDFVNGFSSAYPSKDHNYYSPVKQSQYEVQMDSLTNNSCAKTLNLENPLKTDILDEFFSTSVLSSSADDIEYLPHFDEYMFDS

Target ORF information:

RefSeq Version	XM_020997128.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus ubiquitin specific peptidase like 1 (USPL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020997128.1

ORF Insert Sequence:

ATGATGGATT CCCAGAAGAT TGGAAATGGT TTGCCAGTGA TTGGACAAGA CACTGGTATA 
GGGATATCTT CACTCCACAT GGTGGGGTAT TTGGGAAAAA AGTGTGATTC AGCTAAAGTT 
ACGTCAGATG GGTACTGTCC AGCCTGTAGA GAGAAGGGAA AGATCAAAAC TCTAAAGTCT 
TATCGAATCA GTTTTCAAGA ATCTATCTCT TTGTGTGAAG ATCCACAGTG CATCTATCCA 
TTGGGCTATA AGTCATTAGA TAACATTATA GTCTCTGTTG ATTTGGAAAA TTATCAGCCT 
CCCCGTAAAC AGCTAAAGAG GAAGATCTTG GAAAGCAGCA GTGTTGCTTC ATCTTTGGAA 
CCAAATTCAA AGGAAGATGG CAGCAACACA TGGTTCGACA GTGAATGTAC TGTGAACAGT 
GAGCTTGCTC ACAGACCTGA TGGATATAAT TTGTATAGAA CTTCATCAGA TTTATCTGTT 
GTGTTACAAG ACTGGCAACA GAACCCAAGC AAGGCAGCTG AATCTTTGCA GCATAGTGGG 
ATTTTGGAAG CTGAAATTGC AGACCAGACA GCTGAAGAAC ATCATTTGAT TGTTGGTTCT 
CAGACTGAGA GTATTCCAGA GCCTGAAGTG GATGCAACTA AATTAGAAAT CCTACTGGAG 
AATCGTTGCC TGCCATTTAC TGAATCTCCA TGTCTTCAGT GGAGAAATGC ATATGCTCTC 
TGTTGGTTAG ACTGTATTCT CTCAGCATTA GTGCACTTGG AAGGGTTAAA GAAAGCTGTG 
ACTGAACTAT GTCCTAAAGA AGAATCTATG TTCCAGCAGT TGTATATAAA GTATAATCAA 
GCGATCCTGC TTTTGACTGC TCATCATTTG AATGGAGCTA AAGATGAAAG TTCTATAAGG 
AATGTTTCAG ATAGACTTCG AAAGGCTGAG ACTCACCTAA ATGAAGTCAG AAACAAAATT 
TTTGCTAAGC TTCAACCAAA ACTTCATTGT AAGTTAGGTA ATATGGAAAG TCCTGTGTTT 
GCATTTCCCT TGCTTCTACA AATGGAACCT CTCATTGAAA CGCTCTTCAT GTCATCTTTT 
TCTTGGAACT TTGAGTGTTC TCAGTGTGGA AACAAATATC AACAGAGGTG TTCAAAGAAT 
CTGGTAACCT TCACAAACGT AATACCTGAG TGGCATCCAC TTGATGCTGT CCATTTTGGT 
CCATGTAACA GTTGTAAAAG TAAATTACAA AGAAGAAGAA TGGTATTGGA AAAAGTATCT 
TCAGTATTTA TGCTGCACTT TGTAGAAGGC TTGCCCTGTA ATGACTTGCA GTTCTATTCA 
TTTCATTTTG AAGGCTTTTT CTACCAAATA ACATGTGTGA TTCAATATCA AATGAATGAA 
AAACATTTTA TAAGTTGGAT TTTGAATTCT GATGGAACTT GGCTAGAATG TGATGATTTA 
AAAGGACCAT ACTGTAAAAG ACATCAGAGA TTTGAAGTTC CAGCTTCAGA GATTCACATT 
GTTATCTGGG AAAGGAACAT TTCCAAAATA ATAGATCAAT CCAATATAGA ATTTCCAGTT 
AAAAAGACAG AAAGTTTCAG CCTCAGTGAT TCTAAACCAG AATCTCTGGC ATTGTGTTCT 
GAGGGGGATG CTGCCACTGA AATGTTTTCA GCGTTTAATC AGGAGAATAT TCTAAGTGCT 
CCAACTACTG ATGCACAATA TGTAGTAGCC ACAGATCATG AGTACAGTTT ACTTTCTGGT 
CTAGAAGATT TGGTAGATAA TGATATTATA ACTTTAACAC TCACAGAAAT TCATGTTGAT 
TCTGAGGGCA ATCCAATTGA AAATCAGCCA GTGATAGAAG ATCAGTCTGC GATAAGAGAT 
AAGGCAGTTG CTGAAATGGA CACTTTGCAA AAGCAAGAAT TAGTGTTAGC TTCTTCACAA 
TCAGCTCCAT GTAGGGAAAC ACCTGTCCAA CCTCAAACAC TGCCTACTAG TATCCTAACT 
CAGAATGCTA GTATTGATTT GAAATTGGAT CAGCTGAATA CAGAAAATAC TTCAGATACT 
ATATGCGTGA ATAACACAGT ACATGTGTCT ACTCTTCCGC AAGGAGAGAA GCCATTACAA 
ATTCAAGAGA CAAAAGTTAA TTTTCCTCCC ATTGCCTTGG AGAAGGGTGC TATGTCAAAT 
CCAGTCCTTT CATCAAAGGT TGAAAAACTT ATGTCAAAAC AAAGTGTCTT ATCTCTAGGA 
TCTAATTTGA AAACAGCAAA TAATATAGGA GATTCCCAAA GTGTGGTCCT GGGTTCAACA 
CATAATTCTT CACAGTCCCA GTCACAGAAA AAAAAGTTTG TAGGAAGTTG GGTAAAAGGA 
TTACTGAACA AGGGAGTTTC TTTTATGCCA TCTTGTGCCT CAGCTAATGA AAGAAAGGTC 
CATTTGCAAA AGACAACTGA CTTGAACTCT CTGGTTAAAA GGGCAAATAA TTTTCGGGGC 
TTTAAACCTA AAGGTATAAC AAATCAAACA AGTCCTCAAG CACCCAAGAA AACATCCAGG 
TCTGCATGCA ACTCACTTCC AGTTCTTAAC TCTTCACCAT CAAATATCAG CACATCCTTA 
ATACCTGACG AAGTTCGGGA GGGTACAAAG ATTTTGACTA AATCTTTGCA TAAAAATGTT 
CAGCCCCTCT CTAGTGGGAT ACCTCCTAAG CACAATTTTA ATGGAAATGA AAATCACATC 
TCTTCAGGGA CGTATGAGAG TCAGACTCAT AAACTTCGTC TAAAACTTCT TAAAAAACTT 
AAGGCAAAAA AGCAAAAACT GGCCACTCTT CAGGCTCTCC AAATAAATGG AAAGCCTCCA 
AAGAAAAGTA CAAAACCCTT GCCCCAGGAT GCATCTCCAA ATGAATGTGA ATCACTGCAA 
GGCCTGTTAA TGGAACTCCA GGATCATATC AGTGCCACAG ACAATAAACC TAAAGGTACC 
ACACATTCCA ATATTTCCCT GTGTAATAGC CCAACCCACG AAGAATTTTT AGCAGAATTA 
TTGTCGTCTA CTACAACTGT CGCCTCATCA GAACTACCAA TGAATAGAGA AAATGATTGT 
GAGTATTTGG AGATGGTAGA TAACCATAAC ATGACACCAA CACCTAGTGA CTTTGTCAAT 
GGATTCTCTT CTGCATATCC TAGCAAAGAC CATAATTATT ATAGTCCTGT GAAACAAAGT 
CAGTATGAAG TTCAGATGGA CTCATTAACA AATAATTCTT GTGCTAAAAC GTTAAACTTG 
GAAAATCCCC TAAAGACAGA CATTCTTGAT GAATTTTTTT CCACTTCGGT ATTAAGTTCT 
TCAGCAGATG ACATAGAATA TTTACCTCAT TTTGATGAAT ATATGTTTGA CAGTTGA