USP25 cDNA ORF clone, Phascolarctos cinereus(koala)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP25
Entrez Gene ID	110200874
Full Name	ubiquitin specific peptidase 25
Gene Type	protein-coding
Organism	Phascolarctos cinereus(koala)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020976263.1	XP_020831922.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
XM_020976265.1	XP_020831924.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X4
XM_020976266.1	XP_020831925.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
XM_020976264.1	XP_020831923.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
XM_020976267.1	XP_020831926.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
XM_020976268.1	XP_020831927.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X7
XM_020976262.1	XP_020831921.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
XM_020976269.1	XP_020831928.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X8

Pathways from BioSystems
Gene Ontology

The following USP25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPh281491	XM_020976262.1 Latest version!	Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPh281492	XM_020976263.1 Latest version!	Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPh281493	XM_020976264.1 Latest version!	Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPh281494	XM_020976265.1 Latest version!	Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPh281495	XM_020976266.1 Latest version!	Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPh281496	XM_020976267.1 Latest version!	Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPh281497	XM_020976268.1 Latest version!	Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPh281498	XM_020976269.1 Latest version!	Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP25 ( XM_020976262.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020831921.1 Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPh281491
Clone ID Related Accession (Same CDS sequence)	XM_020976262.1
Accession Version	XM_020976262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344237.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCACCTCCC AGAAGCACCA GCAAACATTT 
TTGAATCAGC TGAGAGAAAT CACGGGCATC AATGATACAC AGATACTACA GCAAGCCTTG 
AAGGATAGCA ACGGAAACTT GGAATTAGCG GTGGCTTTCC TGACTGCAAA GAATGCCAAG 
ACACCTCAGC AAGAGGAAAC AACTTACTAT CAAACAGCCC TTCCTGGAAA TGATAGATAT 
ATCAGTGTGG GAAGCCAGGC AGATACAAGA AAAAAACAGT ATAAGCCAAC CAGAACAAGA 
TGTGCCACAG TTGAGCAAAC ACATTATGGA TCAAAAGATA AGAGGGATAT GGATGTGATT 
GATCTCACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGAAGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCTAT TAGCAGAGTC 
CTTGAAGCCA GCATAGCAGA AAATAAAGCA AGTTTGAAGA GGACGCATAC AGAAGTCTGG 
AGTGATTCTC CAAACCCTTA TGATAGAAAA AGACAGGATA ATTGTCCAGT TGGGCTAAAG 
AATGTTGGAA ATACCTGCTG GTTTAGTGCT GTTATTCAGT CATTATTTAA TCTTCTGGAA 
TTTCGAAGAT TAGTACTGAA TTACAGTCCT CCTGCAAGTG CTCAAGATTT ACCTCGAAAC 
CAAAAGGAAC ATCGGAATTT GCCTTTTATG CGTGAGTTGC GGCATCTGTT TGCACTTCTT 
GTTGGTTCTA AAAGAAAATA TGTTGATCCT TCAAGAGCAG TTGATATTCT TAAAGATGCT 
TTCAAATCGA ATGGCTCACA ACAGCAAGAT GTGAGTGAGT TCACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAGGAAA GGGATGGAGA GAAACCAAAG 
AACCCAATGC TAGAATTGTT CTATGGAAGA TTCCTAGCAG TGGGAGTGCT GGAAGGTAAA 
AAATTTGAAA ATACAGAAAT GTTTGGTCAG TATCCACTTC AAGTCAATGG TTTCAAAGAT 
TTGCATGAAT GCCTAGAAGC TGCCATGATT GAAGGAGAGA TTGAGTCACT ACATTCAGAA 
AACTCAGCAA AATCTGGCCA AGAGCATTGG TTCACAGAAC TTCCACCTGT ATTAACATTT 
GAACTCTCAA GATTTGAATT TAATCAGGCG TTGGGAAGAC CAGAGAAAAT TCACAACAAG 
TTAGAATTCC CTCAAGTTTT ATATCTGGAC AGATATATGC ACAAAAACAG AGAAATAACA 
AGAATTAAAC GAGATGAGAT CAAGAGGCTA AAAGAGTACC TTACAGTATT ACAACAGAGA 
TTAGAAAGAT ATTTAAGCTA TGGTTCTGGC CCCAAACGAT TCCCCTTGGT AGATGTTCTA 
CAGTACGCAC TGGAGTTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCACCTAG TGGTTCCATA CCATCACAGA CATTACCAAG CACAGCAGAG 
CAACAACAGG GAACTCCTTC AGAACTACCA AGCACATCAC CTACACCAAG GTCATTTATA 
CATAAGCCAT TTACACAGTC ACGAATACCT CCAGATTTGC CCATGCATCC AGCACCAAGA 
CATATAACAG AAGAAGAACT TTCTGTACTA GAAGGCTGTT TACATCGCTG GAGGACTGAA 
GTAGAAAATG ACACTAGAGA TTTGCAGGAA AGCATATCTA GAATTCATCG TACAATTGAA 
CAAATGTACT CTGACAAGTC TATGGTACAA GTTCCTTATC GCTTACATGC TGTCTTAGTT 
CATGAAGGCC AAGCTAATGC AGGACACTAT TGGGCATATA TTTTTGATCA TCACCAAGAA 
AGATGGATGA AATATAATGA CATTTCTGTG ACAAAATCAA CATGGGAAGA GCTAGAGCGG 
GACTCTTTTG GTGGCTACAG AAATGCCAGT GCATACTGTT TAATGTATAT TAATGATAAG 
GAACAATTCT TAATAAGAGA AGACTTTCAC AAGGAAACTG GGCAGAAACT TGTCGGAATG 
GAAACATTAC CTACTGATTT AAGGGAATTC GTCAAAGAAG ATAATCAGAG ATTTGAAAAA 
GAGCTAGAGG ATTGGGATCT ACAACTTGCC CAGAAAGCTC AGCAGGAAAA GATTTTAGCA 
TCCCAGAAAA TAAGGGAGCC AGCACCTTCT GTAATGACAG CACAAACAGA AGAACCTGAA 
TACCTAGAGC AGCCATCCAG AACTGATCTC ACCAAGTATT TGAAAGAAGA AACAATGCGA 
GCGATCACCA AGGCATCGTC AGAGCATGAA GACACAAATC CTGAAACTGT CTTACAGCTG 
ACCCCTGAAA AAGCCACCAG TCAACCACCT TCTAACCAGC AAGTTGTAGA GGTGGCGATT 
CCTCACGTAG GGACATTTAT GATTGAGTCA GTGAAGGGGG GTTATGATGA CGAGATCATG 
ATGACACCGA ACATGCAAGG TATTATCATG GCTATAGGTA AATCCAGGAG TGTATATGAC 
AGGTGTGGTC CTGAAGCAGG GTTCTTTAAG GCAATCAAAT TGGAGTATGC AAGGTTGGTA 
AAGCTGGCTC AAGAAGATAC TCCACCAGAA ACAGATTATC GATTGCACCA TGCAGTTGTC 
TATTTTATCC AGAACCAATC ACCAAAGAAA ATAATCGAGA GGACATTGTT GGAACAGTTT 
GGAAATCGCA ATTTGAGTTT TGATGAGAGG TGTCGCAACA TAATGAAAAT TGCTCAAGCT 
AAACTTGAAA TGATAAAGCC AGAAGAAGTA AACATGGAAG AATATGAGAA GTGGCACCAG 
GATTATAGGA ACTTCAGAGA TACAACAATG TATCTCATGA TAGGCCTAGA ACATTTTCAA 
AAAAAGAGTT ACATAAAAGC CATACTGCAT CTTATCTGTG CTTATCAGAA TAATAAGGAG 
CTCTTGTCTA AGGGCCCATA CCGAGGACAT GATGAAGAAT TGATATCACA TTACAGAAGA 
GAATGTTTAC TAAAATTAAA TGAGCATGCA GCAGAACTAT TTGAATCTGG AGATGATCAA 
GAAGTAAACA ATGGATTGAT TATTATGAAT GAGTTTATTG TCCCAAGTTT GCCATTGTTA 
CTGGTGGATG AGATGGAAGA GAAAGATATA GTTGCCGTAG AAGATATGAG AAATCGCTGG 
TGTTCATATC TTGGACAAGA AATGGAACCC AACCTCCAAG AAAAGCTGAC TGACTTCCTG 
CCAAAATTGC TTGATTGTTC TACAGAGATC AAAGGTTTCA ATGAGCTGCC AAAATTACCT 
TCATATTCCA CACATGAACT GTGTGAACGA TTTGCCCGAA TCATGTTGTC CCTCAGTCGA 
ACTCCAGCTG ATGGAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020831921.1
CDS	89..3529
Translation	MTVEQNVLQQSTSQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKQYKPTRTRCATVEQTHYGSKDKRDMDVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPYDRKRQDNCPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYSPPASAQDLPRNQKEHRNLPFMRELRHLFALLVGSKRKYVDPSRAVDILKDAFKSNGSQQQDVSEFTHKLLDWLEDAFQMKAEEERDGEKPKNPMLELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHKNREITRIKRDEIKRLKEYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTAEQQQGTPSELPSTSPTPRSFIHKPFTQSRIPPDLPMHPAPRHITEEELSVLEGCLHRWRTEVENDTRDLQESISRIHRTIEQMYSDKSMVQVPYRLHAVLVHEGQANAGHYWAYIFDHHQERWMKYNDISVTKSTWEELERDSFGGYRNASAYCLMYINDKEQFLIREDFHKETGQKLVGMETLPTDLREFVKEDNQRFEKELEDWDLQLAQKAQQEKILASQKIREPAPSVMTAQTEEPEYLEQPSRTDLTKYLKEETMRAITKASSEHEDTNPETVLQLTPEKATSQPPSNQQVVEVAIPHVGTFMIESVKGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHAVVYFIQNQSPKKIIERTLLEQFGNRNLSFDERCRNIMKIAQAKLEMIKPEEVNMEEYEKWHQDYRNFRDTTMYLMIGLEHFQKKSYIKAILHLICAYQNNKELLSKGPYRGHDEELISHYRRECLLKLNEHAAELFESGDDQEVNNGLIIMNEFIVPSLPLLLVDEMEEKDIVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKGFNELPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_020976262.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020976262.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCACCTCCC AGAAGCACCA GCAAACATTT 
TTGAATCAGC TGAGAGAAAT CACGGGCATC AATGATACAC AGATACTACA GCAAGCCTTG 
AAGGATAGCA ACGGAAACTT GGAATTAGCG GTGGCTTTCC TGACTGCAAA GAATGCCAAG 
ACACCTCAGC AAGAGGAAAC AACTTACTAT CAAACAGCCC TTCCTGGAAA TGATAGATAT 
ATCAGTGTGG GAAGCCAGGC AGATACAAGA AAAAAACAGT ATAAGCCAAC CAGAACAAGA 
TGTGCCACAG TTGAGCAAAC ACATTATGGA TCAAAAGATA AGAGGGATAT GGATGTGATT 
GATCTCACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGAAGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCTAT TAGCAGAGTC 
CTTGAAGCCA GCATAGCAGA AAATAAAGCA AGTTTGAAGA GGACGCATAC AGAAGTCTGG 
AGTGATTCTC CAAACCCTTA TGATAGAAAA AGACAGGATA ATTGTCCAGT TGGGCTAAAG 
AATGTTGGAA ATACCTGCTG GTTTAGTGCT GTTATTCAGT CATTATTTAA TCTTCTGGAA 
TTTCGAAGAT TAGTACTGAA TTACAGTCCT CCTGCAAGTG CTCAAGATTT ACCTCGAAAC 
CAAAAGGAAC ATCGGAATTT GCCTTTTATG CGTGAGTTGC GGCATCTGTT TGCACTTCTT 
GTTGGTTCTA AAAGAAAATA TGTTGATCCT TCAAGAGCAG TTGATATTCT TAAAGATGCT 
TTCAAATCGA ATGGCTCACA ACAGCAAGAT GTGAGTGAGT TCACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAGGAAA GGGATGGAGA GAAACCAAAG 
AACCCAATGC TAGAATTGTT CTATGGAAGA TTCCTAGCAG TGGGAGTGCT GGAAGGTAAA 
AAATTTGAAA ATACAGAAAT GTTTGGTCAG TATCCACTTC AAGTCAATGG TTTCAAAGAT 
TTGCATGAAT GCCTAGAAGC TGCCATGATT GAAGGAGAGA TTGAGTCACT ACATTCAGAA 
AACTCAGCAA AATCTGGCCA AGAGCATTGG TTCACAGAAC TTCCACCTGT ATTAACATTT 
GAACTCTCAA GATTTGAATT TAATCAGGCG TTGGGAAGAC CAGAGAAAAT TCACAACAAG 
TTAGAATTCC CTCAAGTTTT ATATCTGGAC AGATATATGC ACAAAAACAG AGAAATAACA 
AGAATTAAAC GAGATGAGAT CAAGAGGCTA AAAGAGTACC TTACAGTATT ACAACAGAGA 
TTAGAAAGAT ATTTAAGCTA TGGTTCTGGC CCCAAACGAT TCCCCTTGGT AGATGTTCTA 
CAGTACGCAC TGGAGTTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCACCTAG TGGTTCCATA CCATCACAGA CATTACCAAG CACAGCAGAG 
CAACAACAGG GAACTCCTTC AGAACTACCA AGCACATCAC CTACACCAAG GTCATTTATA 
CATAAGCCAT TTACACAGTC ACGAATACCT CCAGATTTGC CCATGCATCC AGCACCAAGA 
CATATAACAG AAGAAGAACT TTCTGTACTA GAAGGCTGTT TACATCGCTG GAGGACTGAA 
GTAGAAAATG ACACTAGAGA TTTGCAGGAA AGCATATCTA GAATTCATCG TACAATTGAA 
CAAATGTACT CTGACAAGTC TATGGTACAA GTTCCTTATC GCTTACATGC TGTCTTAGTT 
CATGAAGGCC AAGCTAATGC AGGACACTAT TGGGCATATA TTTTTGATCA TCACCAAGAA 
AGATGGATGA AATATAATGA CATTTCTGTG ACAAAATCAA CATGGGAAGA GCTAGAGCGG 
GACTCTTTTG GTGGCTACAG AAATGCCAGT GCATACTGTT TAATGTATAT TAATGATAAG 
GAACAATTCT TAATAAGAGA AGACTTTCAC AAGGAAACTG GGCAGAAACT TGTCGGAATG 
GAAACATTAC CTACTGATTT AAGGGAATTC GTCAAAGAAG ATAATCAGAG ATTTGAAAAA 
GAGCTAGAGG ATTGGGATCT ACAACTTGCC CAGAAAGCTC AGCAGGAAAA GATTTTAGCA 
TCCCAGAAAA TAAGGGAGCC AGCACCTTCT GTAATGACAG CACAAACAGA AGAACCTGAA 
TACCTAGAGC AGCCATCCAG AACTGATCTC ACCAAGTATT TGAAAGAAGA AACAATGCGA 
GCGATCACCA AGGCATCGTC AGAGCATGAA GACACAAATC CTGAAACTGT CTTACAGCTG 
ACCCCTGAAA AAGCCACCAG TCAACCACCT TCTAACCAGC AAGTTGTAGA GGTGGCGATT 
CCTCACGTAG GGACATTTAT GATTGAGTCA GTGAAGGGGG GTTATGATGA CGAGATCATG 
ATGACACCGA ACATGCAAGG TATTATCATG GCTATAGGTA AATCCAGGAG TGTATATGAC 
AGGTGTGGTC CTGAAGCAGG GTTCTTTAAG GCAATCAAAT TGGAGTATGC AAGGTTGGTA 
AAGCTGGCTC AAGAAGATAC TCCACCAGAA ACAGATTATC GATTGCACCA TGCAGTTGTC 
TATTTTATCC AGAACCAATC ACCAAAGAAA ATAATCGAGA GGACATTGTT GGAACAGTTT 
GGAAATCGCA ATTTGAGTTT TGATGAGAGG TGTCGCAACA TAATGAAAAT TGCTCAAGCT 
AAACTTGAAA TGATAAAGCC AGAAGAAGTA AACATGGAAG AATATGAGAA GTGGCACCAG 
GATTATAGGA ACTTCAGAGA TACAACAATG TATCTCATGA TAGGCCTAGA ACATTTTCAA 
AAAAAGAGTT ACATAAAAGC CATACTGCAT CTTATCTGTG CTTATCAGAA TAATAAGGAG 
CTCTTGTCTA AGGGCCCATA CCGAGGACAT GATGAAGAAT TGATATCACA TTACAGAAGA 
GAATGTTTAC TAAAATTAAA TGAGCATGCA GCAGAACTAT TTGAATCTGG AGATGATCAA 
GAAGTAAACA ATGGATTGAT TATTATGAAT GAGTTTATTG TCCCAAGTTT GCCATTGTTA 
CTGGTGGATG AGATGGAAGA GAAAGATATA GTTGCCGTAG AAGATATGAG AAATCGCTGG 
TGTTCATATC TTGGACAAGA AATGGAACCC AACCTCCAAG AAAAGCTGAC TGACTTCCTG 
CCAAAATTGC TTGATTGTTC TACAGAGATC AAAGGTTTCA ATGAGCTGCC AAAATTACCT 
TCATATTCCA CACATGAACT GTGTGAACGA TTTGCCCGAA TCATGTTGTC CCTCAGTCGA 
ACTCCAGCTG ATGGAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_020976263.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020831922.1 Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPh281492
Clone ID Related Accession (Same CDS sequence)	XM_020976263.1
Accession Version	XM_020976263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344237.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCACCTCCC AGAAGCACCA GCAAACATTT 
TTGAATCAGC TGAGAGAAAT CACGGGCATC AATGATACAC AGATACTACA GCAAGCCTTG 
AAGGATAGCA ACGGAAACTT GGAATTAGCG GTGGCTTTCC TGACTGCAAA GAATGCCAAG 
ACACCTCAGC AAGAGGAAAC AACTTACTAT CAAACAGCCC TTCCTGGAAA TGATAGATAT 
ATCAGTGTGG GAAGCCAGGC AGATACAAGA AAAAAACAGT ATAAGCCAAC CAGAACAAGA 
TGTGCCACAG TTGAGCAAAC ACATTATGGA TCAAAAGATA AGAGGGATAT GGATGTGATT 
GATCTCACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGAAGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCTAT TAGCAGAGTC 
CTTGAAGCCA GCATAGCAGA AAATAAAGCA AGTTTGAAGA GGACGCATAC AGAAGTCTGG 
AGTGATTCTC CAAACCCTTA TGATAGAAAA AGACAGGATA ATTGTCCAGT TGGGCTAAAG 
AATGTTGGAA ATACCTGCTG GTTTAGTGCT GTTATTCAGT CATTATTTAA TCTTCTGGAA 
TTTCGAAGAT TAGTACTGAA TTACAGTCCT CCTGCAAGTG CTCAAGATTT ACCTCGAAAC 
CAAAAGGAAC ATCGGAATTT GCCTTTTATG CGTGAGTTGC GGCATCTGTT TGCACTTCTT 
GTTGGTTCTA AAAGAAAATA TGTTGATCCT TCAAGAGCAG TTGATATTCT TAAAGATGCT 
TTCAAATCGA ATGGCTCACA ACAGCAAGAT GTGAGTGAGT TCACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAGGAAA GGGATGGAGA GAAACCAAAG 
AACCCAATGC TAGAATTGTT CTATGGAAGA TTCCTAGCAG TGGGAGTGCT GGAAGGTAAA 
AAATTTGAAA ATACAGAAAT GTTTGGTCAG TATCCACTTC AAGTCAATGG TTTCAAAGAT 
TTGCATGAAT GCCTAGAAGC TGCCATGATT GAAGGAGAGA TTGAGTCACT ACATTCAGAA 
AACTCAGCAA AATCTGGCCA AGAGCATTGG TTCACAGAAC TTCCACCTGT ATTAACATTT 
GAACTCTCAA GATTTGAATT TAATCAGGCG TTGGGAAGAC CAGAGAAAAT TCACAACAAG 
TTAGAATTCC CTCAAGTTTT ATATCTGGAC AGATATATGC ACAAAAACAG AGAAATAACA 
AGAATTAAAC GAGATGAGAT CAAGAGGCTA AAAGAGTACC TTACAGTATT ACAACAGAGA 
TTAGAAAGAT ATTTAAGCTA TGGTTCTGGC CCCAAACGAT TCCCCTTGGT AGATGTTCTA 
CAGTACGCAC TGGAGTTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCACCTAG TGGTTCCATA CCATCACAGA CATTACCAAG CACAGCAGAG 
CAACAACAGG GAACTCCTTC AGAACTACCA AGCACATCAC CTACACCAAG GTCATTTATA 
CATAAGCCAT TTACACAGTC ACGAATACCT CCAGATTTGC CCATGCATCC AGCACCAAGA 
CATATAACAG AAGAAGAACT TTCTGTACTA GAAGGCTGTT TACATCGCTG GAGGACTGAA 
GTAGAAAATG ACACTAGAGA TTTGCAGGAA AGCATATCTA GAATTCATCG TACAATTGAA 
CAAATGTACT CTGACAAGTC TATGGTACAA GTTCCTTATC GCTTACATGC TGTCTTAGTT 
CATGAAGGCC AAGCTAATGC AGGACACTAT TGGGCATATA TTTTTGATCA TCACCAAGAA 
AGATGGATGA AATATAATGA CATTTCTGTG ACAAAATCAA CATGGGAAGA GCTAGAGCGG 
GACTCTTTTG GTGGCTACAG AAATGCCAGT GCATACTGTT TAATGTATAT TAATGATAAG 
GAACAATTCT TAATAAGAGA CTTTCACAAG GAAACTGGGC AGAAACTTGT CGGAATGGAA 
ACATTACCTA CTGATTTAAG GGAATTCGTC AAAGAAGATA ATCAGAGATT TGAAAAAGAG 
CTAGAGGATT GGGATCTACA ACTTGCCCAG AAAGCTCAGC AGGAAAAGAT TTTAGCATCC 
CAGAAAATAA GGGAGCCAGC ACCTTCTGTA ATGACAGCAC AAACAGAAGA ACCTGAATAC 
CTAGAGCAGC CATCCAGAAC TGATCTCACC AAGTATTTGA AAGAAGAAAC AATGCGAGCG 
ATCACCAAGG CATCGTCAGA GCATGAAGAC ACAAATCCTG AAACTGTCTT ACAGCTGACC 
CCTGAAAAAG CCACCAGTCA ACCACCTTCT AACCAGCAAG TTGTAGAGGT GGCGATTCCT 
CACGTAGGGA CATTTATGAT TGAGTCAGTG AAGGGGGGTT ATGATGACGA GATCATGATG 
ACACCGAACA TGCAAGGTAT TATCATGGCT ATAGGTAAAT CCAGGAGTGT ATATGACAGG 
TGTGGTCCTG AAGCAGGGTT CTTTAAGGCA ATCAAATTGG AGTATGCAAG GTTGGTAAAG 
CTGGCTCAAG AAGATACTCC ACCAGAAACA GATTATCGAT TGCACCATGC AGTTGTCTAT 
TTTATCCAGA ACCAATCACC AAAGAAAATA ATCGAGAGGA CATTGTTGGA ACAGTTTGGA 
AATCGCAATT TGAGTTTTGA TGAGAGGTGT CGCAACATAA TGAAAATTGC TCAAGCTAAA 
CTTGAAATGA TAAAGCCAGA AGAAGTAAAC ATGGAAGAAT ATGAGAAGTG GCACCAGGAT 
TATAGGAACT TCAGAGATAC AACAATGTAT CTCATGATAG GCCTAGAACA TTTTCAAAAA 
AAGAGTTACA TAAAAGCCAT ACTGCATCTT ATCTGTGCTT ATCAGAATAA TAAGGAGCTC 
TTGTCTAAGG GCCCATACCG AGGACATGAT GAAGAATTGA TATCACATTA CAGAAGAGAA 
TGTTTACTAA AATTAAATGA GCATGCAGCA GAACTATTTG AATCTGGAGA TGATCAAGAA 
GTAAACAATG GATTGATTAT TATGAATGAG TTTATTGTCC CAAGTTTGCC ATTGTTACTG 
GTGGATGAGA TGGAAGAGAA AGATATAGTT GCCGTAGAAG ATATGAGAAA TCGCTGGTGT 
TCATATCTTG GACAAGAAAT GGAACCCAAC CTCCAAGAAA AGCTGACTGA CTTCCTGCCA 
AAATTGCTTG ATTGTTCTAC AGAGATCAAA GGTTTCAATG AGCTGCCAAA ATTACCTTCA 
TATTCCACAC ATGAACTGTG TGAACGATTT GCCCGAATCA TGTTGTCCCT CAGTCGAACT 
CCAGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020831922.1
CDS	89..3526
Translation	MTVEQNVLQQSTSQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKQYKPTRTRCATVEQTHYGSKDKRDMDVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPYDRKRQDNCPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYSPPASAQDLPRNQKEHRNLPFMRELRHLFALLVGSKRKYVDPSRAVDILKDAFKSNGSQQQDVSEFTHKLLDWLEDAFQMKAEEERDGEKPKNPMLELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHKNREITRIKRDEIKRLKEYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTAEQQQGTPSELPSTSPTPRSFIHKPFTQSRIPPDLPMHPAPRHITEEELSVLEGCLHRWRTEVENDTRDLQESISRIHRTIEQMYSDKSMVQVPYRLHAVLVHEGQANAGHYWAYIFDHHQERWMKYNDISVTKSTWEELERDSFGGYRNASAYCLMYINDKEQFLIRDFHKETGQKLVGMETLPTDLREFVKEDNQRFEKELEDWDLQLAQKAQQEKILASQKIREPAPSVMTAQTEEPEYLEQPSRTDLTKYLKEETMRAITKASSEHEDTNPETVLQLTPEKATSQPPSNQQVVEVAIPHVGTFMIESVKGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHAVVYFIQNQSPKKIIERTLLEQFGNRNLSFDERCRNIMKIAQAKLEMIKPEEVNMEEYEKWHQDYRNFRDTTMYLMIGLEHFQKKSYIKAILHLICAYQNNKELLSKGPYRGHDEELISHYRRECLLKLNEHAAELFESGDDQEVNNGLIIMNEFIVPSLPLLLVDEMEEKDIVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKGFNELPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_020976263.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020976263.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCACCTCCC AGAAGCACCA GCAAACATTT 
TTGAATCAGC TGAGAGAAAT CACGGGCATC AATGATACAC AGATACTACA GCAAGCCTTG 
AAGGATAGCA ACGGAAACTT GGAATTAGCG GTGGCTTTCC TGACTGCAAA GAATGCCAAG 
ACACCTCAGC AAGAGGAAAC AACTTACTAT CAAACAGCCC TTCCTGGAAA TGATAGATAT 
ATCAGTGTGG GAAGCCAGGC AGATACAAGA AAAAAACAGT ATAAGCCAAC CAGAACAAGA 
TGTGCCACAG TTGAGCAAAC ACATTATGGA TCAAAAGATA AGAGGGATAT GGATGTGATT 
GATCTCACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGAAGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCTAT TAGCAGAGTC 
CTTGAAGCCA GCATAGCAGA AAATAAAGCA AGTTTGAAGA GGACGCATAC AGAAGTCTGG 
AGTGATTCTC CAAACCCTTA TGATAGAAAA AGACAGGATA ATTGTCCAGT TGGGCTAAAG 
AATGTTGGAA ATACCTGCTG GTTTAGTGCT GTTATTCAGT CATTATTTAA TCTTCTGGAA 
TTTCGAAGAT TAGTACTGAA TTACAGTCCT CCTGCAAGTG CTCAAGATTT ACCTCGAAAC 
CAAAAGGAAC ATCGGAATTT GCCTTTTATG CGTGAGTTGC GGCATCTGTT TGCACTTCTT 
GTTGGTTCTA AAAGAAAATA TGTTGATCCT TCAAGAGCAG TTGATATTCT TAAAGATGCT 
TTCAAATCGA ATGGCTCACA ACAGCAAGAT GTGAGTGAGT TCACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAGGAAA GGGATGGAGA GAAACCAAAG 
AACCCAATGC TAGAATTGTT CTATGGAAGA TTCCTAGCAG TGGGAGTGCT GGAAGGTAAA 
AAATTTGAAA ATACAGAAAT GTTTGGTCAG TATCCACTTC AAGTCAATGG TTTCAAAGAT 
TTGCATGAAT GCCTAGAAGC TGCCATGATT GAAGGAGAGA TTGAGTCACT ACATTCAGAA 
AACTCAGCAA AATCTGGCCA AGAGCATTGG TTCACAGAAC TTCCACCTGT ATTAACATTT 
GAACTCTCAA GATTTGAATT TAATCAGGCG TTGGGAAGAC CAGAGAAAAT TCACAACAAG 
TTAGAATTCC CTCAAGTTTT ATATCTGGAC AGATATATGC ACAAAAACAG AGAAATAACA 
AGAATTAAAC GAGATGAGAT CAAGAGGCTA AAAGAGTACC TTACAGTATT ACAACAGAGA 
TTAGAAAGAT ATTTAAGCTA TGGTTCTGGC CCCAAACGAT TCCCCTTGGT AGATGTTCTA 
CAGTACGCAC TGGAGTTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCACCTAG TGGTTCCATA CCATCACAGA CATTACCAAG CACAGCAGAG 
CAACAACAGG GAACTCCTTC AGAACTACCA AGCACATCAC CTACACCAAG GTCATTTATA 
CATAAGCCAT TTACACAGTC ACGAATACCT CCAGATTTGC CCATGCATCC AGCACCAAGA 
CATATAACAG AAGAAGAACT TTCTGTACTA GAAGGCTGTT TACATCGCTG GAGGACTGAA 
GTAGAAAATG ACACTAGAGA TTTGCAGGAA AGCATATCTA GAATTCATCG TACAATTGAA 
CAAATGTACT CTGACAAGTC TATGGTACAA GTTCCTTATC GCTTACATGC TGTCTTAGTT 
CATGAAGGCC AAGCTAATGC AGGACACTAT TGGGCATATA TTTTTGATCA TCACCAAGAA 
AGATGGATGA AATATAATGA CATTTCTGTG ACAAAATCAA CATGGGAAGA GCTAGAGCGG 
GACTCTTTTG GTGGCTACAG AAATGCCAGT GCATACTGTT TAATGTATAT TAATGATAAG 
GAACAATTCT TAATAAGAGA CTTTCACAAG GAAACTGGGC AGAAACTTGT CGGAATGGAA 
ACATTACCTA CTGATTTAAG GGAATTCGTC AAAGAAGATA ATCAGAGATT TGAAAAAGAG 
CTAGAGGATT GGGATCTACA ACTTGCCCAG AAAGCTCAGC AGGAAAAGAT TTTAGCATCC 
CAGAAAATAA GGGAGCCAGC ACCTTCTGTA ATGACAGCAC AAACAGAAGA ACCTGAATAC 
CTAGAGCAGC CATCCAGAAC TGATCTCACC AAGTATTTGA AAGAAGAAAC AATGCGAGCG 
ATCACCAAGG CATCGTCAGA GCATGAAGAC ACAAATCCTG AAACTGTCTT ACAGCTGACC 
CCTGAAAAAG CCACCAGTCA ACCACCTTCT AACCAGCAAG TTGTAGAGGT GGCGATTCCT 
CACGTAGGGA CATTTATGAT TGAGTCAGTG AAGGGGGGTT ATGATGACGA GATCATGATG 
ACACCGAACA TGCAAGGTAT TATCATGGCT ATAGGTAAAT CCAGGAGTGT ATATGACAGG 
TGTGGTCCTG AAGCAGGGTT CTTTAAGGCA ATCAAATTGG AGTATGCAAG GTTGGTAAAG 
CTGGCTCAAG AAGATACTCC ACCAGAAACA GATTATCGAT TGCACCATGC AGTTGTCTAT 
TTTATCCAGA ACCAATCACC AAAGAAAATA ATCGAGAGGA CATTGTTGGA ACAGTTTGGA 
AATCGCAATT TGAGTTTTGA TGAGAGGTGT CGCAACATAA TGAAAATTGC TCAAGCTAAA 
CTTGAAATGA TAAAGCCAGA AGAAGTAAAC ATGGAAGAAT ATGAGAAGTG GCACCAGGAT 
TATAGGAACT TCAGAGATAC AACAATGTAT CTCATGATAG GCCTAGAACA TTTTCAAAAA 
AAGAGTTACA TAAAAGCCAT ACTGCATCTT ATCTGTGCTT ATCAGAATAA TAAGGAGCTC 
TTGTCTAAGG GCCCATACCG AGGACATGAT GAAGAATTGA TATCACATTA CAGAAGAGAA 
TGTTTACTAA AATTAAATGA GCATGCAGCA GAACTATTTG AATCTGGAGA TGATCAAGAA 
GTAAACAATG GATTGATTAT TATGAATGAG TTTATTGTCC CAAGTTTGCC ATTGTTACTG 
GTGGATGAGA TGGAAGAGAA AGATATAGTT GCCGTAGAAG ATATGAGAAA TCGCTGGTGT 
TCATATCTTG GACAAGAAAT GGAACCCAAC CTCCAAGAAA AGCTGACTGA CTTCCTGCCA 
AAATTGCTTG ATTGTTCTAC AGAGATCAAA GGTTTCAATG AGCTGCCAAA ATTACCTTCA 
TATTCCACAC ATGAACTGTG TGAACGATTT GCCCGAATCA TGTTGTCCCT CAGTCGAACT 
CCAGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_020976264.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020831923.1 Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPh281493
Clone ID Related Accession (Same CDS sequence)	XM_020976264.1
Accession Version	XM_020976264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344237.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCACCTCCC AGAAGCACCA GCAAACATTT 
TTGAATCAGC TGAGAGAAAT CACGGGCATC AATGATACAC AGATACTACA GCAAGCCTTG 
AAGGATAGCA ACGGAAACTT GGAATTAGCG GTGGCTTTCC TGACTGCAAA GAATGCCAAG 
ACACCTCAGC AAGAGGAAAC AACTTACTAT CAAACAGCCC TTCCTGGAAA TGATAGATAT 
ATCAGTGTGG GAAGCCAGGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GAAGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCTATTAGC AGAGTCCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCAAGTT TGAAGAGGAC GCATACAGAA GTCTGGAGTG ATTCTCCAAA CCCTTATGAT 
AGAAAAAGAC AGGATAATTG TCCAGTTGGG CTAAAGAATG TTGGAAATAC CTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT CTGGAATTTC GAAGATTAGT ACTGAATTAC 
AGTCCTCCTG CAAGTGCTCA AGATTTACCT CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGTTGCGGCA TCTGTTTGCA CTTCTTGTTG GTTCTAAAAG AAAATATGTT 
GATCCTTCAA GAGCAGTTGA TATTCTTAAA GATGCTTTCA AATCGAATGG CTCACAACAG 
CAAGATGTGA GTGAGTTCAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAAAGGGA TGGAGAGAAA CCAAAGAACC CAATGCTAGA ATTGTTCTAT 
GGAAGATTCC TAGCAGTGGG AGTGCTGGAA GGTAAAAAAT TTGAAAATAC AGAAATGTTT 
GGTCAGTATC CACTTCAAGT CAATGGTTTC AAAGATTTGC ATGAATGCCT AGAAGCTGCC 
ATGATTGAAG GAGAGATTGA GTCACTACAT TCAGAAAACT CAGCAAAATC TGGCCAAGAG 
CATTGGTTCA CAGAACTTCC ACCTGTATTA ACATTTGAAC TCTCAAGATT TGAATTTAAT 
CAGGCGTTGG GAAGACCAGA GAAAATTCAC AACAAGTTAG AATTCCCTCA AGTTTTATAT 
CTGGACAGAT ATATGCACAA AAACAGAGAA ATAACAAGAA TTAAACGAGA TGAGATCAAG 
AGGCTAAAAG AGTACCTTAC AGTATTACAA CAGAGATTAG AAAGATATTT AAGCTATGGT 
TCTGGCCCCA AACGATTCCC CTTGGTAGAT GTTCTACAGT ACGCACTGGA GTTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA GCAGAGCAAC AACAGGGAAC TCCTTCAGAA 
CTACCAAGCA CATCACCTAC ACCAAGGTCA TTTATACATA AGCCATTTAC ACAGTCACGA 
ATACCTCCAG ATTTGCCCAT GCATCCAGCA CCAAGACATA TAACAGAAGA AGAACTTTCT 
GTACTAGAAG GCTGTTTACA TCGCTGGAGG ACTGAAGTAG AAAATGACAC TAGAGATTTG 
CAGGAAAGCA TATCTAGAAT TCATCGTACA ATTGAACAAA TGTACTCTGA CAAGTCTATG 
GTACAAGTTC CTTATCGCTT ACATGCTGTC TTAGTTCATG AAGGCCAAGC TAATGCAGGA 
CACTATTGGG CATATATTTT TGATCATCAC CAAGAAAGAT GGATGAAATA TAATGACATT 
TCTGTGACAA AATCAACATG GGAAGAGCTA GAGCGGGACT CTTTTGGTGG CTACAGAAAT 
GCCAGTGCAT ACTGTTTAAT GTATATTAAT GATAAGGAAC AATTCTTAAT AAGAGAAGAC 
TTTCACAAGG AAACTGGGCA GAAACTTGTC GGAATGGAAA CATTACCTAC TGATTTAAGG 
GAATTCGTCA AAGAAGATAA TCAGAGATTT GAAAAAGAGC TAGAGGATTG GGATCTACAA 
CTTGCCCAGA AAGCTCAGCA GGAAAAGATT TTAGCATCCC AGAAAATAAG GGAGCCAGCA 
CCTTCTGTAA TGACAGCACA AACAGAAGAA CCTGAATACC TAGAGCAGCC ATCCAGAACT 
GATCTCACCA AGTATTTGAA AGAAGAAACA ATGCGAGCGA TCACCAAGGC ATCGTCAGAG 
CATGAAGACA CAAATCCTGA AACTGTCTTA CAGCTGACCC CTGAAAAAGC CACCAGTCAA 
CCACCTTCTA ACCAGCAAGT TGTAGAGGTG GCGATTCCTC ACGTAGGGAC ATTTATGATT 
GAGTCAGTGA AGGGGGGTTA TGATGACGAG ATCATGATGA CACCGAACAT GCAAGGTATT 
ATCATGGCTA TAGGTAAATC CAGGAGTGTA TATGACAGGT GTGGTCCTGA AGCAGGGTTC 
TTTAAGGCAA TCAAATTGGA GTATGCAAGG TTGGTAAAGC TGGCTCAAGA AGATACTCCA 
CCAGAAACAG ATTATCGATT GCACCATGCA GTTGTCTATT TTATCCAGAA CCAATCACCA 
AAGAAAATAA TCGAGAGGAC ATTGTTGGAA CAGTTTGGAA ATCGCAATTT GAGTTTTGAT 
GAGAGGTGTC GCAACATAAT GAAAATTGCT CAAGCTAAAC TTGAAATGAT AAAGCCAGAA 
GAAGTAAACA TGGAAGAATA TGAGAAGTGG CACCAGGATT ATAGGAACTT CAGAGATACA 
ACAATGTATC TCATGATAGG CCTAGAACAT TTTCAAAAAA AGAGTTACAT AAAAGCCATA 
CTGCATCTTA TCTGTGCTTA TCAGAATAAT AAGGAGCTCT TGTCTAAGGG CCCATACCGA 
GGACATGATG AAGAATTGAT ATCACATTAC AGAAGAGAAT GTTTACTAAA ATTAAATGAG 
CATGCAGCAG AACTATTTGA ATCTGGAGAT GATCAAGAAG TAAACAATGG ATTGATTATT 
ATGAATGAGT TTATTGTCCC AAGTTTGCCA TTGTTACTGG TGGATGAGAT GGAAGAGAAA 
GATATAGTTG CCGTAGAAGA TATGAGAAAT CGCTGGTGTT CATATCTTGG ACAAGAAATG 
GAACCCAACC TCCAAGAAAA GCTGACTGAC TTCCTGCCAA AATTGCTTGA TTGTTCTACA 
GAGATCAAAG GTTTCAATGA GCTGCCAAAA TTACCTTCAT ATTCCACACA TGAACTGTGT 
GAACGATTTG CCCGAATCAT GTTGTCCCTC AGTCGAACTC CAGCTGATGG AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020831923.1
CDS	89..3445
Translation	MTVEQNVLQQSTSQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPYDRKRQDNCPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYSPPASAQDLPRNQKEHRNLPFMRELRHLFALLVGSKRKYVDPSRAVDILKDAFKSNGSQQQDVSEFTHKLLDWLEDAFQMKAEEERDGEKPKNPMLELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHKNREITRIKRDEIKRLKEYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTAEQQQGTPSELPSTSPTPRSFIHKPFTQSRIPPDLPMHPAPRHITEEELSVLEGCLHRWRTEVENDTRDLQESISRIHRTIEQMYSDKSMVQVPYRLHAVLVHEGQANAGHYWAYIFDHHQERWMKYNDISVTKSTWEELERDSFGGYRNASAYCLMYINDKEQFLIREDFHKETGQKLVGMETLPTDLREFVKEDNQRFEKELEDWDLQLAQKAQQEKILASQKIREPAPSVMTAQTEEPEYLEQPSRTDLTKYLKEETMRAITKASSEHEDTNPETVLQLTPEKATSQPPSNQQVVEVAIPHVGTFMIESVKGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHAVVYFIQNQSPKKIIERTLLEQFGNRNLSFDERCRNIMKIAQAKLEMIKPEEVNMEEYEKWHQDYRNFRDTTMYLMIGLEHFQKKSYIKAILHLICAYQNNKELLSKGPYRGHDEELISHYRRECLLKLNEHAAELFESGDDQEVNNGLIIMNEFIVPSLPLLLVDEMEEKDIVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKGFNELPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_020976264.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020976264.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCACCTCCC AGAAGCACCA GCAAACATTT 
TTGAATCAGC TGAGAGAAAT CACGGGCATC AATGATACAC AGATACTACA GCAAGCCTTG 
AAGGATAGCA ACGGAAACTT GGAATTAGCG GTGGCTTTCC TGACTGCAAA GAATGCCAAG 
ACACCTCAGC AAGAGGAAAC AACTTACTAT CAAACAGCCC TTCCTGGAAA TGATAGATAT 
ATCAGTGTGG GAAGCCAGGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GAAGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCTATTAGC AGAGTCCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCAAGTT TGAAGAGGAC GCATACAGAA GTCTGGAGTG ATTCTCCAAA CCCTTATGAT 
AGAAAAAGAC AGGATAATTG TCCAGTTGGG CTAAAGAATG TTGGAAATAC CTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT CTGGAATTTC GAAGATTAGT ACTGAATTAC 
AGTCCTCCTG CAAGTGCTCA AGATTTACCT CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGTTGCGGCA TCTGTTTGCA CTTCTTGTTG GTTCTAAAAG AAAATATGTT 
GATCCTTCAA GAGCAGTTGA TATTCTTAAA GATGCTTTCA AATCGAATGG CTCACAACAG 
CAAGATGTGA GTGAGTTCAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAAAGGGA TGGAGAGAAA CCAAAGAACC CAATGCTAGA ATTGTTCTAT 
GGAAGATTCC TAGCAGTGGG AGTGCTGGAA GGTAAAAAAT TTGAAAATAC AGAAATGTTT 
GGTCAGTATC CACTTCAAGT CAATGGTTTC AAAGATTTGC ATGAATGCCT AGAAGCTGCC 
ATGATTGAAG GAGAGATTGA GTCACTACAT TCAGAAAACT CAGCAAAATC TGGCCAAGAG 
CATTGGTTCA CAGAACTTCC ACCTGTATTA ACATTTGAAC TCTCAAGATT TGAATTTAAT 
CAGGCGTTGG GAAGACCAGA GAAAATTCAC AACAAGTTAG AATTCCCTCA AGTTTTATAT 
CTGGACAGAT ATATGCACAA AAACAGAGAA ATAACAAGAA TTAAACGAGA TGAGATCAAG 
AGGCTAAAAG AGTACCTTAC AGTATTACAA CAGAGATTAG AAAGATATTT AAGCTATGGT 
TCTGGCCCCA AACGATTCCC CTTGGTAGAT GTTCTACAGT ACGCACTGGA GTTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA GCAGAGCAAC AACAGGGAAC TCCTTCAGAA 
CTACCAAGCA CATCACCTAC ACCAAGGTCA TTTATACATA AGCCATTTAC ACAGTCACGA 
ATACCTCCAG ATTTGCCCAT GCATCCAGCA CCAAGACATA TAACAGAAGA AGAACTTTCT 
GTACTAGAAG GCTGTTTACA TCGCTGGAGG ACTGAAGTAG AAAATGACAC TAGAGATTTG 
CAGGAAAGCA TATCTAGAAT TCATCGTACA ATTGAACAAA TGTACTCTGA CAAGTCTATG 
GTACAAGTTC CTTATCGCTT ACATGCTGTC TTAGTTCATG AAGGCCAAGC TAATGCAGGA 
CACTATTGGG CATATATTTT TGATCATCAC CAAGAAAGAT GGATGAAATA TAATGACATT 
TCTGTGACAA AATCAACATG GGAAGAGCTA GAGCGGGACT CTTTTGGTGG CTACAGAAAT 
GCCAGTGCAT ACTGTTTAAT GTATATTAAT GATAAGGAAC AATTCTTAAT AAGAGAAGAC 
TTTCACAAGG AAACTGGGCA GAAACTTGTC GGAATGGAAA CATTACCTAC TGATTTAAGG 
GAATTCGTCA AAGAAGATAA TCAGAGATTT GAAAAAGAGC TAGAGGATTG GGATCTACAA 
CTTGCCCAGA AAGCTCAGCA GGAAAAGATT TTAGCATCCC AGAAAATAAG GGAGCCAGCA 
CCTTCTGTAA TGACAGCACA AACAGAAGAA CCTGAATACC TAGAGCAGCC ATCCAGAACT 
GATCTCACCA AGTATTTGAA AGAAGAAACA ATGCGAGCGA TCACCAAGGC ATCGTCAGAG 
CATGAAGACA CAAATCCTGA AACTGTCTTA CAGCTGACCC CTGAAAAAGC CACCAGTCAA 
CCACCTTCTA ACCAGCAAGT TGTAGAGGTG GCGATTCCTC ACGTAGGGAC ATTTATGATT 
GAGTCAGTGA AGGGGGGTTA TGATGACGAG ATCATGATGA CACCGAACAT GCAAGGTATT 
ATCATGGCTA TAGGTAAATC CAGGAGTGTA TATGACAGGT GTGGTCCTGA AGCAGGGTTC 
TTTAAGGCAA TCAAATTGGA GTATGCAAGG TTGGTAAAGC TGGCTCAAGA AGATACTCCA 
CCAGAAACAG ATTATCGATT GCACCATGCA GTTGTCTATT TTATCCAGAA CCAATCACCA 
AAGAAAATAA TCGAGAGGAC ATTGTTGGAA CAGTTTGGAA ATCGCAATTT GAGTTTTGAT 
GAGAGGTGTC GCAACATAAT GAAAATTGCT CAAGCTAAAC TTGAAATGAT AAAGCCAGAA 
GAAGTAAACA TGGAAGAATA TGAGAAGTGG CACCAGGATT ATAGGAACTT CAGAGATACA 
ACAATGTATC TCATGATAGG CCTAGAACAT TTTCAAAAAA AGAGTTACAT AAAAGCCATA 
CTGCATCTTA TCTGTGCTTA TCAGAATAAT AAGGAGCTCT TGTCTAAGGG CCCATACCGA 
GGACATGATG AAGAATTGAT ATCACATTAC AGAAGAGAAT GTTTACTAAA ATTAAATGAG 
CATGCAGCAG AACTATTTGA ATCTGGAGAT GATCAAGAAG TAAACAATGG ATTGATTATT 
ATGAATGAGT TTATTGTCCC AAGTTTGCCA TTGTTACTGG TGGATGAGAT GGAAGAGAAA 
GATATAGTTG CCGTAGAAGA TATGAGAAAT CGCTGGTGTT CATATCTTGG ACAAGAAATG 
GAACCCAACC TCCAAGAAAA GCTGACTGAC TTCCTGCCAA AATTGCTTGA TTGTTCTACA 
GAGATCAAAG GTTTCAATGA GCTGCCAAAA TTACCTTCAT ATTCCACACA TGAACTGTGT 
GAACGATTTG CCCGAATCAT GTTGTCCCTC AGTCGAACTC CAGCTGATGG AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_020976265.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020831924.1 Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPh281494
Clone ID Related Accession (Same CDS sequence)	XM_020976265.1
Accession Version	XM_020976265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344237.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCACCTCCC AGAAGCACCA GCAAACATTT 
TTGAATCAGC TGAGAGAAAT CACGGGCATC AATGATACAC AGATACTACA GCAAGCCTTG 
AAGGATAGCA ACGGAAACTT GGAATTAGCG GTGGCTTTCC TGACTGCAAA GAATGCCAAG 
ACACCTCAGC AAGAGGAAAC AACTTACTAT CAAACAGCCC TTCCTGGAAA TGATAGATAT 
ATCAGTGTGG GAAGCCAGGC AGATACAAGA AAAAAACAGT ATAAGCCAAC CAGAACAAGA 
TGTGCCACAG TTGAGCAAAC ACATTATGGA TCAAAAGATA AGAGGGATAT GGATGTGATT 
GATCTCACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGAAGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCTAT TAGCAGAGTC 
CTTGAAGCCA GCATAGCAGA AAATAAAGCA AGTTTGAAGA GGACGCATAC AGAAGTCTGG 
AGTGATTCTC CAAACCCTTA TGATAGAAAA AGACAGGATA ATTGTCCAGT TGGGCTAAAG 
AATGTTGGAA ATACCTGCTG GTTTAGTGCT GTTATTCAGT CATTATTTAA TCTTCTGGAA 
TTTCGAAGAT TAGTACTGAA TTACAGTCCT CCTGCAAGTG CTCAAGATTT ACCTCGAAAC 
CAAAAGGAAC ATCGGAATTT GCCTTTTATG CGTGAGTTGC GGCATCTGTT TGCACTTCTT 
GTTGGTTCTA AAAGAAAATA TGTTGATCCT TCAAGAGCAG TTGATATTCT TAAAGATGCT 
TTCAAATCGA ATGGCTCACA ACAGCAAGAT GTGAGTGAGT TCACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAGGAAA GGGATGGAGA GAAACCAAAG 
AACCCAATGC TAGAATTGTT CTATGGAAGA TTCCTAGCAG TGGGAGTGCT GGAAGGTAAA 
AAATTTGAAA ATACAGAAAT GTTTGGTCAG TATCCACTTC AAGTCAATGG TTTCAAAGAT 
TTGCATGAAT GCCTAGAAGC TGCCATGATT GAAGGAGAGA TTGAGTCACT ACATTCAGAA 
AACTCAGCAA AATCTGGCCA AGAGCATTGG TTCACAGAAC TTCCACCTGT ATTAACATTT 
GAACTCTCAA GATTTGAATT TAATCAGGCG TTGGGAAGAC CAGAGAAAAT TCACAACAAG 
TTAGAATTCC CTCAAGTTTT ATATCTGGAC AGATATATGC ACAAAAACAG AGAAATAACA 
AGAATTAAAC GAGATGAGAT CAAGAGGCTA AAAGAGTACC TTACAGTATT ACAACAGAGA 
TTAGAAAGAT ATTTAAGCTA TGGTTCTGGC CCCAAACGAT TCCCCTTGGT AGATGTTCTA 
CAGTACGCAC TGGAGTTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCACCTAG TGGTTCCATA CCATCACAGA CATTACCAAG CACAGCAGAG 
CAACAACAGG GAACTCCTTC AGAACTACCA AGCACATCAC CTACACCAAG GTCATTTATA 
CATAAGCCAT TTACACAGTC ACGAATACCT CCAGATTTGC CCATGCATCC AGCACCAAGA 
CATATAACAG AAGAAGAACT TTCTGTACTA GAAGGCTGTT TACATCGCTG GAGGACTGAA 
GTAGAAAATG ACACTAGAGA TTTGCAGGAA AGCATATCTA GAATTCATCG TACAATTGAA 
CAAATGTACT CTGACAAGTC TATGGTACAA GTTCCTTATC GCTTACATGC TGTCTTAGTT 
CATGAAGGCC AAGCTAATGC AGGACACTAT TGGGCATATA TTTTTGATCA TCACCAAGAA 
AGATGGATGA AATATAATGA CATTTCTGTG ACAAAATCAA CATGGGAAGA GCTAGAGCGG 
GACTCTTTTG GTGGCTACAG AAATGCCAGT GCATACTGTT TAATGTATAT TAATGATAAG 
GAACAATTCT TAATAAGAGA AGACTTTCAC AAGGAAACTG GGCAGAAACT TGTCGGAATG 
GAAACATTAC CTACTGATTT AAGGGAATTC GTCAAAGAAG ATAATCAGAG ATTTGAAAAA 
GAGCTAGAGG ATTGGGATCT ACAACTTGCC CAGAAAGCTC AGCAGGAAAA GATTTTAGCA 
TCCCAGAAAA TAAGGGAGCC AGCACCTTCT GTAATGACAG CACAAACAGA AGAACCTGAA 
TACCTAGAGC AGCCATCCAG AACTGATCTC ACCAAGTATT TGAAAGAAGA AACAATGCGA 
GCGATCACCA AGGCATCGTC AGAGCATGAA GACACAAATC CTGAAACTGT CTTACAGCTG 
ATCATGATGA CACCGAACAT GCAAGGTATT ATCATGGCTA TAGGTAAATC CAGGAGTGTA 
TATGACAGGT GTGGTCCTGA AGCAGGGTTC TTTAAGGCAA TCAAATTGGA GTATGCAAGG 
TTGGTAAAGC TGGCTCAAGA AGATACTCCA CCAGAAACAG ATTATCGATT GCACCATGCA 
GTTGTCTATT TTATCCAGAA CCAATCACCA AAGAAAATAA TCGAGAGGAC ATTGTTGGAA 
CAGTTTGGAA ATCGCAATTT GAGTTTTGAT GAGAGGTGTC GCAACATAAT GAAAATTGCT 
CAAGCTAAAC TTGAAATGAT AAAGCCAGAA GAAGTAAACA TGGAAGAATA TGAGAAGTGG 
CACCAGGATT ATAGGAACTT CAGAGATACA ACAATGTATC TCATGATAGG CCTAGAACAT 
TTTCAAAAAA AGAGTTACAT AAAAGCCATA CTGCATCTTA TCTGTGCTTA TCAGAATAAT 
AAGGAGCTCT TGTCTAAGGG CCCATACCGA GGACATGATG AAGAATTGAT ATCACATTAC 
AGAAGAGAAT GTTTACTAAA ATTAAATGAG CATGCAGCAG AACTATTTGA ATCTGGAGAT 
GATCAAGAAG TAAACAATGG ATTGATTATT ATGAATGAGT TTATTGTCCC AAGTTTGCCA 
TTGTTACTGG TGGATGAGAT GGAAGAGAAA GATATAGTTG CCGTAGAAGA TATGAGAAAT 
CGCTGGTGTT CATATCTTGG ACAAGAAATG GAACCCAACC TCCAAGAAAA GCTGACTGAC 
TTCCTGCCAA AATTGCTTGA TTGTTCTACA GAGATCAAAG GTTTCAATGA GCTGCCAAAA 
TTACCTTCAT ATTCCACACA TGAACTGTGT GAACGATTTG CCCGAATCAT GTTGTCCCTC 
AGTCGAACTC CAGCTGATGG AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020831924.1
CDS	89..3415
Translation	MTVEQNVLQQSTSQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKQYKPTRTRCATVEQTHYGSKDKRDMDVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPYDRKRQDNCPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYSPPASAQDLPRNQKEHRNLPFMRELRHLFALLVGSKRKYVDPSRAVDILKDAFKSNGSQQQDVSEFTHKLLDWLEDAFQMKAEEERDGEKPKNPMLELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHKNREITRIKRDEIKRLKEYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTAEQQQGTPSELPSTSPTPRSFIHKPFTQSRIPPDLPMHPAPRHITEEELSVLEGCLHRWRTEVENDTRDLQESISRIHRTIEQMYSDKSMVQVPYRLHAVLVHEGQANAGHYWAYIFDHHQERWMKYNDISVTKSTWEELERDSFGGYRNASAYCLMYINDKEQFLIREDFHKETGQKLVGMETLPTDLREFVKEDNQRFEKELEDWDLQLAQKAQQEKILASQKIREPAPSVMTAQTEEPEYLEQPSRTDLTKYLKEETMRAITKASSEHEDTNPETVLQLIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHAVVYFIQNQSPKKIIERTLLEQFGNRNLSFDERCRNIMKIAQAKLEMIKPEEVNMEEYEKWHQDYRNFRDTTMYLMIGLEHFQKKSYIKAILHLICAYQNNKELLSKGPYRGHDEELISHYRRECLLKLNEHAAELFESGDDQEVNNGLIIMNEFIVPSLPLLLVDEMEEKDIVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKGFNELPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_020976265.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020976265.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCACCTCCC AGAAGCACCA GCAAACATTT 
TTGAATCAGC TGAGAGAAAT CACGGGCATC AATGATACAC AGATACTACA GCAAGCCTTG 
AAGGATAGCA ACGGAAACTT GGAATTAGCG GTGGCTTTCC TGACTGCAAA GAATGCCAAG 
ACACCTCAGC AAGAGGAAAC AACTTACTAT CAAACAGCCC TTCCTGGAAA TGATAGATAT 
ATCAGTGTGG GAAGCCAGGC AGATACAAGA AAAAAACAGT ATAAGCCAAC CAGAACAAGA 
TGTGCCACAG TTGAGCAAAC ACATTATGGA TCAAAAGATA AGAGGGATAT GGATGTGATT 
GATCTCACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGAAGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCTAT TAGCAGAGTC 
CTTGAAGCCA GCATAGCAGA AAATAAAGCA AGTTTGAAGA GGACGCATAC AGAAGTCTGG 
AGTGATTCTC CAAACCCTTA TGATAGAAAA AGACAGGATA ATTGTCCAGT TGGGCTAAAG 
AATGTTGGAA ATACCTGCTG GTTTAGTGCT GTTATTCAGT CATTATTTAA TCTTCTGGAA 
TTTCGAAGAT TAGTACTGAA TTACAGTCCT CCTGCAAGTG CTCAAGATTT ACCTCGAAAC 
CAAAAGGAAC ATCGGAATTT GCCTTTTATG CGTGAGTTGC GGCATCTGTT TGCACTTCTT 
GTTGGTTCTA AAAGAAAATA TGTTGATCCT TCAAGAGCAG TTGATATTCT TAAAGATGCT 
TTCAAATCGA ATGGCTCACA ACAGCAAGAT GTGAGTGAGT TCACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAGGAAA GGGATGGAGA GAAACCAAAG 
AACCCAATGC TAGAATTGTT CTATGGAAGA TTCCTAGCAG TGGGAGTGCT GGAAGGTAAA 
AAATTTGAAA ATACAGAAAT GTTTGGTCAG TATCCACTTC AAGTCAATGG TTTCAAAGAT 
TTGCATGAAT GCCTAGAAGC TGCCATGATT GAAGGAGAGA TTGAGTCACT ACATTCAGAA 
AACTCAGCAA AATCTGGCCA AGAGCATTGG TTCACAGAAC TTCCACCTGT ATTAACATTT 
GAACTCTCAA GATTTGAATT TAATCAGGCG TTGGGAAGAC CAGAGAAAAT TCACAACAAG 
TTAGAATTCC CTCAAGTTTT ATATCTGGAC AGATATATGC ACAAAAACAG AGAAATAACA 
AGAATTAAAC GAGATGAGAT CAAGAGGCTA AAAGAGTACC TTACAGTATT ACAACAGAGA 
TTAGAAAGAT ATTTAAGCTA TGGTTCTGGC CCCAAACGAT TCCCCTTGGT AGATGTTCTA 
CAGTACGCAC TGGAGTTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCACCTAG TGGTTCCATA CCATCACAGA CATTACCAAG CACAGCAGAG 
CAACAACAGG GAACTCCTTC AGAACTACCA AGCACATCAC CTACACCAAG GTCATTTATA 
CATAAGCCAT TTACACAGTC ACGAATACCT CCAGATTTGC CCATGCATCC AGCACCAAGA 
CATATAACAG AAGAAGAACT TTCTGTACTA GAAGGCTGTT TACATCGCTG GAGGACTGAA 
GTAGAAAATG ACACTAGAGA TTTGCAGGAA AGCATATCTA GAATTCATCG TACAATTGAA 
CAAATGTACT CTGACAAGTC TATGGTACAA GTTCCTTATC GCTTACATGC TGTCTTAGTT 
CATGAAGGCC AAGCTAATGC AGGACACTAT TGGGCATATA TTTTTGATCA TCACCAAGAA 
AGATGGATGA AATATAATGA CATTTCTGTG ACAAAATCAA CATGGGAAGA GCTAGAGCGG 
GACTCTTTTG GTGGCTACAG AAATGCCAGT GCATACTGTT TAATGTATAT TAATGATAAG 
GAACAATTCT TAATAAGAGA AGACTTTCAC AAGGAAACTG GGCAGAAACT TGTCGGAATG 
GAAACATTAC CTACTGATTT AAGGGAATTC GTCAAAGAAG ATAATCAGAG ATTTGAAAAA 
GAGCTAGAGG ATTGGGATCT ACAACTTGCC CAGAAAGCTC AGCAGGAAAA GATTTTAGCA 
TCCCAGAAAA TAAGGGAGCC AGCACCTTCT GTAATGACAG CACAAACAGA AGAACCTGAA 
TACCTAGAGC AGCCATCCAG AACTGATCTC ACCAAGTATT TGAAAGAAGA AACAATGCGA 
GCGATCACCA AGGCATCGTC AGAGCATGAA GACACAAATC CTGAAACTGT CTTACAGCTG 
ATCATGATGA CACCGAACAT GCAAGGTATT ATCATGGCTA TAGGTAAATC CAGGAGTGTA 
TATGACAGGT GTGGTCCTGA AGCAGGGTTC TTTAAGGCAA TCAAATTGGA GTATGCAAGG 
TTGGTAAAGC TGGCTCAAGA AGATACTCCA CCAGAAACAG ATTATCGATT GCACCATGCA 
GTTGTCTATT TTATCCAGAA CCAATCACCA AAGAAAATAA TCGAGAGGAC ATTGTTGGAA 
CAGTTTGGAA ATCGCAATTT GAGTTTTGAT GAGAGGTGTC GCAACATAAT GAAAATTGCT 
CAAGCTAAAC TTGAAATGAT AAAGCCAGAA GAAGTAAACA TGGAAGAATA TGAGAAGTGG 
CACCAGGATT ATAGGAACTT CAGAGATACA ACAATGTATC TCATGATAGG CCTAGAACAT 
TTTCAAAAAA AGAGTTACAT AAAAGCCATA CTGCATCTTA TCTGTGCTTA TCAGAATAAT 
AAGGAGCTCT TGTCTAAGGG CCCATACCGA GGACATGATG AAGAATTGAT ATCACATTAC 
AGAAGAGAAT GTTTACTAAA ATTAAATGAG CATGCAGCAG AACTATTTGA ATCTGGAGAT 
GATCAAGAAG TAAACAATGG ATTGATTATT ATGAATGAGT TTATTGTCCC AAGTTTGCCA 
TTGTTACTGG TGGATGAGAT GGAAGAGAAA GATATAGTTG CCGTAGAAGA TATGAGAAAT 
CGCTGGTGTT CATATCTTGG ACAAGAAATG GAACCCAACC TCCAAGAAAA GCTGACTGAC 
TTCCTGCCAA AATTGCTTGA TTGTTCTACA GAGATCAAAG GTTTCAATGA GCTGCCAAAA 
TTACCTTCAT ATTCCACACA TGAACTGTGT GAACGATTTG CCCGAATCAT GTTGTCCCTC 
AGTCGAACTC CAGCTGATGG AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_020976266.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020831925.1 Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X5, mRNA.

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CloneID	OPh281495
Clone ID Related Accession (Same CDS sequence)	XM_020976266.1
Accession Version	XM_020976266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344237.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCACCTCCC AGAAGCACCA GCAAACATTT 
TTGAATCAGC TGAGAGAAAT CACGGGCATC AATGATACAC AGATACTACA GCAAGCCTTG 
AAGGATAGCA ACGGAAACTT GGAATTAGCG GTGGCTTTCC TGACTGCAAA GAATGCCAAG 
ACACCTCAGC AAGAGGAAAC AACTTACTAT CAAACAGCCC TTCCTGGAAA TGATAGATAT 
ATCAGTGTGG GAAGCCAGGC AGATACAAGA AAAAAACAGT ATAAGCCAAC CAGAACAAGA 
TGTGCCACAG TTGAGCAAAC ACATTATGGA TCAAAAGATA AGAGGGATAT GGATGTGATT 
GATCTCACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGAAGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCTAT TAGCAGAGTC 
CTTGAAGCCA GCATAGCAGA AAATAAAGCA AGTTTGAAGA GGACGCATAC AGAAGTCTGG 
AGTGATTCTC CAAACCCTTA TGATAGAAAA AGACAGGATA ATTGTCCAGT TGGGCTAAAG 
AATGTTGGAA ATACCTGCTG GTTTAGTGCT GTTATTCAGT CATTATTTAA TCTTCTGGAA 
TTTCGAAGAT TAGTACTGAA TTACAGTCCT CCTGCAAGTG CTCAAGATTT ACCTCGAAAC 
CAAAAGGAAC ATCGGAATTT GCCTTTTATG CGTGAGTTGC GGCATCTGTT TGCACTTCTT 
GTTGGTTCTA AAAGAAAATA TGTTGATCCT TCAAGAGCAG TTGATATTCT TAAAGATGCT 
TTCAAATCGA ATGGCTCACA ACAGCAAGAT GTGAGTGAGT TCACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAGGAAA GGGATGGAGA GAAACCAAAG 
AACCCAATGC TAGAATTGTT CTATGGAAGA TTCCTAGCAG TGGGAGTGCT GGAAGGTAAA 
AAATTTGAAA ATACAGAAAT GTTTGGTCAG TATCCACTTC AAGTCAATGG TTTCAAAGAT 
TTGCATGAAT GCCTAGAAGC TGCCATGATT GAAGGAGAGA TTGAGTCACT ACATTCAGAA 
AACTCAGCAA AATCTGGCCA AGAGCATTGG TTCACAGAAC TTCCACCTGT ATTAACATTT 
GAACTCTCAA GATTTGAATT TAATCAGGCG TTGGGAAGAC CAGAGAAAAT TCACAACAAG 
TTAGAATTCC CTCAAGTTTT ATATCTGGAC AGATATATGC ACAAAAACAG AGAAATAACA 
AGAATTAAAC GAGATGAGAT CAAGAGGCTA AAAGAGTACC TTACAGTATT ACAACAGAGA 
TTAGAAAGCA CAGCAGAGCA ACAACAGGGA ACTCCTTCAG AACTACCAAG CACATCACCT 
ACACCAAGGT CATTTATACA TAAGCCATTT ACACAGTCAC GAATACCTCC AGATTTGCCC 
ATGCATCCAG CACCAAGACA TATAACAGAA GAAGAACTTT CTGTACTAGA AGGCTGTTTA 
CATCGCTGGA GGACTGAAGT AGAAAATGAC ACTAGAGATT TGCAGGAAAG CATATCTAGA 
ATTCATCGTA CAATTGAACA AATGTACTCT GACAAGTCTA TGGTACAAGT TCCTTATCGC 
TTACATGCTG TCTTAGTTCA TGAAGGCCAA GCTAATGCAG GACACTATTG GGCATATATT 
TTTGATCATC ACCAAGAAAG ATGGATGAAA TATAATGACA TTTCTGTGAC AAAATCAACA 
TGGGAAGAGC TAGAGCGGGA CTCTTTTGGT GGCTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTATATTA ATGATAAGGA ACAATTCTTA ATAAGAGAAG ACTTTCACAA GGAAACTGGG 
CAGAAACTTG TCGGAATGGA AACATTACCT ACTGATTTAA GGGAATTCGT CAAAGAAGAT 
AATCAGAGAT TTGAAAAAGA GCTAGAGGAT TGGGATCTAC AACTTGCCCA GAAAGCTCAG 
CAGGAAAAGA TTTTAGCATC CCAGAAAATA AGGGAGCCAG CACCTTCTGT AATGACAGCA 
CAAACAGAAG AACCTGAATA CCTAGAGCAG CCATCCAGAA CTGATCTCAC CAAGTATTTG 
AAAGAAGAAA CAATGCGAGC GATCACCAAG GCATCGTCAG AGCATGAAGA CACAAATCCT 
GAAACTGTCT TACAGCTGAC CCCTGAAAAA GCCACCAGTC AACCACCTTC TAACCAGCAA 
GTTGTAGAGG TGGCGATTCC TCACGTAGGG ACATTTATGA TTGAGTCAGT GAAGGGGGGT 
TATGATGACG AGATCATGAT GACACCGAAC ATGCAAGGTA TTATCATGGC TATAGGTAAA 
TCCAGGAGTG TATATGACAG GTGTGGTCCT GAAGCAGGGT TCTTTAAGGC AATCAAATTG 
GAGTATGCAA GGTTGGTAAA GCTGGCTCAA GAAGATACTC CACCAGAAAC AGATTATCGA 
TTGCACCATG CAGTTGTCTA TTTTATCCAG AACCAATCAC CAAAGAAAAT AATCGAGAGG 
ACATTGTTGG AACAGTTTGG AAATCGCAAT TTGAGTTTTG ATGAGAGGTG TCGCAACATA 
ATGAAAATTG CTCAAGCTAA ACTTGAAATG ATAAAGCCAG AAGAAGTAAA CATGGAAGAA 
TATGAGAAGT GGCACCAGGA TTATAGGAAC TTCAGAGATA CAACAATGTA TCTCATGATA 
GGCCTAGAAC ATTTTCAAAA AAAGAGTTAC ATAAAAGCCA TACTGCATCT TATCTGTGCT 
TATCAGAATA ATAAGGAGCT CTTGTCTAAG GGCCCATACC GAGGACATGA TGAAGAATTG 
ATATCACATT ACAGAAGAGA ATGTTTACTA AAATTAAATG AGCATGCAGC AGAACTATTT 
GAATCTGGAG ATGATCAAGA AGTAAACAAT GGATTGATTA TTATGAATGA GTTTATTGTC 
CCAAGTTTGC CATTGTTACT GGTGGATGAG ATGGAAGAGA AAGATATAGT TGCCGTAGAA 
GATATGAGAA ATCGCTGGTG TTCATATCTT GGACAAGAAA TGGAACCCAA CCTCCAAGAA 
AAGCTGACTG ACTTCCTGCC AAAATTGCTT GATTGTTCTA CAGAGATCAA AGGTTTCAAT 
GAGCTGCCAA AATTACCTTC ATATTCCACA CATGAACTGT GTGAACGATT TGCCCGAATC 
ATGTTGTCCC TCAGTCGAAC TCCAGCTGAT GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020831925.1
CDS	89..3367
Translation	MTVEQNVLQQSTSQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKQYKPTRTRCATVEQTHYGSKDKRDMDVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPYDRKRQDNCPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYSPPASAQDLPRNQKEHRNLPFMRELRHLFALLVGSKRKYVDPSRAVDILKDAFKSNGSQQQDVSEFTHKLLDWLEDAFQMKAEEERDGEKPKNPMLELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHKNREITRIKRDEIKRLKEYLTVLQQRLESTAEQQQGTPSELPSTSPTPRSFIHKPFTQSRIPPDLPMHPAPRHITEEELSVLEGCLHRWRTEVENDTRDLQESISRIHRTIEQMYSDKSMVQVPYRLHAVLVHEGQANAGHYWAYIFDHHQERWMKYNDISVTKSTWEELERDSFGGYRNASAYCLMYINDKEQFLIREDFHKETGQKLVGMETLPTDLREFVKEDNQRFEKELEDWDLQLAQKAQQEKILASQKIREPAPSVMTAQTEEPEYLEQPSRTDLTKYLKEETMRAITKASSEHEDTNPETVLQLTPEKATSQPPSNQQVVEVAIPHVGTFMIESVKGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHAVVYFIQNQSPKKIIERTLLEQFGNRNLSFDERCRNIMKIAQAKLEMIKPEEVNMEEYEKWHQDYRNFRDTTMYLMIGLEHFQKKSYIKAILHLICAYQNNKELLSKGPYRGHDEELISHYRRECLLKLNEHAAELFESGDDQEVNNGLIIMNEFIVPSLPLLLVDEMEEKDIVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKGFNELPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_020976266.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020976266.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCACCTCCC AGAAGCACCA GCAAACATTT 
TTGAATCAGC TGAGAGAAAT CACGGGCATC AATGATACAC AGATACTACA GCAAGCCTTG 
AAGGATAGCA ACGGAAACTT GGAATTAGCG GTGGCTTTCC TGACTGCAAA GAATGCCAAG 
ACACCTCAGC AAGAGGAAAC AACTTACTAT CAAACAGCCC TTCCTGGAAA TGATAGATAT 
ATCAGTGTGG GAAGCCAGGC AGATACAAGA AAAAAACAGT ATAAGCCAAC CAGAACAAGA 
TGTGCCACAG TTGAGCAAAC ACATTATGGA TCAAAAGATA AGAGGGATAT GGATGTGATT 
GATCTCACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGAAGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCTAT TAGCAGAGTC 
CTTGAAGCCA GCATAGCAGA AAATAAAGCA AGTTTGAAGA GGACGCATAC AGAAGTCTGG 
AGTGATTCTC CAAACCCTTA TGATAGAAAA AGACAGGATA ATTGTCCAGT TGGGCTAAAG 
AATGTTGGAA ATACCTGCTG GTTTAGTGCT GTTATTCAGT CATTATTTAA TCTTCTGGAA 
TTTCGAAGAT TAGTACTGAA TTACAGTCCT CCTGCAAGTG CTCAAGATTT ACCTCGAAAC 
CAAAAGGAAC ATCGGAATTT GCCTTTTATG CGTGAGTTGC GGCATCTGTT TGCACTTCTT 
GTTGGTTCTA AAAGAAAATA TGTTGATCCT TCAAGAGCAG TTGATATTCT TAAAGATGCT 
TTCAAATCGA ATGGCTCACA ACAGCAAGAT GTGAGTGAGT TCACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAGGAAA GGGATGGAGA GAAACCAAAG 
AACCCAATGC TAGAATTGTT CTATGGAAGA TTCCTAGCAG TGGGAGTGCT GGAAGGTAAA 
AAATTTGAAA ATACAGAAAT GTTTGGTCAG TATCCACTTC AAGTCAATGG TTTCAAAGAT 
TTGCATGAAT GCCTAGAAGC TGCCATGATT GAAGGAGAGA TTGAGTCACT ACATTCAGAA 
AACTCAGCAA AATCTGGCCA AGAGCATTGG TTCACAGAAC TTCCACCTGT ATTAACATTT 
GAACTCTCAA GATTTGAATT TAATCAGGCG TTGGGAAGAC CAGAGAAAAT TCACAACAAG 
TTAGAATTCC CTCAAGTTTT ATATCTGGAC AGATATATGC ACAAAAACAG AGAAATAACA 
AGAATTAAAC GAGATGAGAT CAAGAGGCTA AAAGAGTACC TTACAGTATT ACAACAGAGA 
TTAGAAAGCA CAGCAGAGCA ACAACAGGGA ACTCCTTCAG AACTACCAAG CACATCACCT 
ACACCAAGGT CATTTATACA TAAGCCATTT ACACAGTCAC GAATACCTCC AGATTTGCCC 
ATGCATCCAG CACCAAGACA TATAACAGAA GAAGAACTTT CTGTACTAGA AGGCTGTTTA 
CATCGCTGGA GGACTGAAGT AGAAAATGAC ACTAGAGATT TGCAGGAAAG CATATCTAGA 
ATTCATCGTA CAATTGAACA AATGTACTCT GACAAGTCTA TGGTACAAGT TCCTTATCGC 
TTACATGCTG TCTTAGTTCA TGAAGGCCAA GCTAATGCAG GACACTATTG GGCATATATT 
TTTGATCATC ACCAAGAAAG ATGGATGAAA TATAATGACA TTTCTGTGAC AAAATCAACA 
TGGGAAGAGC TAGAGCGGGA CTCTTTTGGT GGCTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTATATTA ATGATAAGGA ACAATTCTTA ATAAGAGAAG ACTTTCACAA GGAAACTGGG 
CAGAAACTTG TCGGAATGGA AACATTACCT ACTGATTTAA GGGAATTCGT CAAAGAAGAT 
AATCAGAGAT TTGAAAAAGA GCTAGAGGAT TGGGATCTAC AACTTGCCCA GAAAGCTCAG 
CAGGAAAAGA TTTTAGCATC CCAGAAAATA AGGGAGCCAG CACCTTCTGT AATGACAGCA 
CAAACAGAAG AACCTGAATA CCTAGAGCAG CCATCCAGAA CTGATCTCAC CAAGTATTTG 
AAAGAAGAAA CAATGCGAGC GATCACCAAG GCATCGTCAG AGCATGAAGA CACAAATCCT 
GAAACTGTCT TACAGCTGAC CCCTGAAAAA GCCACCAGTC AACCACCTTC TAACCAGCAA 
GTTGTAGAGG TGGCGATTCC TCACGTAGGG ACATTTATGA TTGAGTCAGT GAAGGGGGGT 
TATGATGACG AGATCATGAT GACACCGAAC ATGCAAGGTA TTATCATGGC TATAGGTAAA 
TCCAGGAGTG TATATGACAG GTGTGGTCCT GAAGCAGGGT TCTTTAAGGC AATCAAATTG 
GAGTATGCAA GGTTGGTAAA GCTGGCTCAA GAAGATACTC CACCAGAAAC AGATTATCGA 
TTGCACCATG CAGTTGTCTA TTTTATCCAG AACCAATCAC CAAAGAAAAT AATCGAGAGG 
ACATTGTTGG AACAGTTTGG AAATCGCAAT TTGAGTTTTG ATGAGAGGTG TCGCAACATA 
ATGAAAATTG CTCAAGCTAA ACTTGAAATG ATAAAGCCAG AAGAAGTAAA CATGGAAGAA 
TATGAGAAGT GGCACCAGGA TTATAGGAAC TTCAGAGATA CAACAATGTA TCTCATGATA 
GGCCTAGAAC ATTTTCAAAA AAAGAGTTAC ATAAAAGCCA TACTGCATCT TATCTGTGCT 
TATCAGAATA ATAAGGAGCT CTTGTCTAAG GGCCCATACC GAGGACATGA TGAAGAATTG 
ATATCACATT ACAGAAGAGA ATGTTTACTA AAATTAAATG AGCATGCAGC AGAACTATTT 
GAATCTGGAG ATGATCAAGA AGTAAACAAT GGATTGATTA TTATGAATGA GTTTATTGTC 
CCAAGTTTGC CATTGTTACT GGTGGATGAG ATGGAAGAGA AAGATATAGT TGCCGTAGAA 
GATATGAGAA ATCGCTGGTG TTCATATCTT GGACAAGAAA TGGAACCCAA CCTCCAAGAA 
AAGCTGACTG ACTTCCTGCC AAAATTGCTT GATTGTTCTA CAGAGATCAA AGGTTTCAAT 
GAGCTGCCAA AATTACCTTC ATATTCCACA CATGAACTGT GTGAACGATT TGCCCGAATC 
ATGTTGTCCC TCAGTCGAAC TCCAGCTGAT GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_020976267.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020831926.1 Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPh281496
Clone ID Related Accession (Same CDS sequence)	XM_020976267.1
Accession Version	XM_020976267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344237.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCACCTCCC AGAAGCACCA GCAAACATTT 
TTGAATCAGC TGAGAGAAAT CACGGGCATC AATGATACAC AGATACTACA GCAAGCCTTG 
AAGGATAGCA ACGGAAACTT GGAATTAGCG GTGGCTTTCC TGACTGCAAA GAATGCCAAG 
ACACCTCAGC AAGAGGAAAC AACTTACTAT CAAACAGCCC TTCCTGGAAA TGATAGATAT 
ATCAGTGTGG GAAGCCAGGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GAAGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCTATTAGC AGAGTCCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCAAGTT TGAAGAGGAC GCATACAGAA GTCTGGAGTG ATTCTCCAAA CCCTTATGAT 
AGAAAAAGAC AGGATAATTG TCCAGTTGGG CTAAAGAATG TTGGAAATAC CTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT CTGGAATTTC GAAGATTAGT ACTGAATTAC 
AGTCCTCCTG CAAGTGCTCA AGATTTACCT CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGTTGCGGCA TCTGTTTGCA CTTCTTGTTG GTTCTAAAAG AAAATATGTT 
GATCCTTCAA GAGCAGTTGA TATTCTTAAA GATGCTTTCA AATCGAATGG CTCACAACAG 
CAAGATGTGA GTGAGTTCAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAAAGGGA TGGAGAGAAA CCAAAGAACC CAATGCTAGA ATTGTTCTAT 
GGAAGATTCC TAGCAGTGGG AGTGCTGGAA GGTAAAAAAT TTGAAAATAC AGAAATGTTT 
GGTCAGTATC CACTTCAAGT CAATGGTTTC AAAGATTTGC ATGAATGCCT AGAAGCTGCC 
ATGATTGAAG GAGAGATTGA GTCACTACAT TCAGAAAACT CAGCAAAATC TGGCCAAGAG 
CATTGGTTCA CAGAACTTCC ACCTGTATTA ACATTTGAAC TCTCAAGATT TGAATTTAAT 
CAGGCGTTGG GAAGACCAGA GAAAATTCAC AACAAGTTAG AATTCCCTCA AGTTTTATAT 
CTGGACAGAT ATATGCACAA AAACAGAGAA ATAACAAGAA TTAAACGAGA TGAGATCAAG 
AGGCTAAAAG AGTACCTTAC AGTATTACAA CAGAGATTAG AAAGATATTT AAGCTATGGT 
TCTGGCCCCA AACGATTCCC CTTGGTAGAT GTTCTACAGT ACGCACTGGA GTTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA GCAGAGCAAC AACAGGGAAC TCCTTCAGAA 
CTACCAAGCA CATCACCTAC ACCAAGGTCA TTTATACATA AGCCATTTAC ACAGTCACGA 
ATACCTCCAG ATTTGCCCAT GCATCCAGCA CCAAGACATA TAACAGAAGA AGAACTTTCT 
GTACTAGAAG GCTGTTTACA TCGCTGGAGG ACTGAAGTAG AAAATGACAC TAGAGATTTG 
CAGGAAAGCA TATCTAGAAT TCATCGTACA ATTGAACAAA TGTACTCTGA CAAGTCTATG 
GTACAAGTTC CTTATCGCTT ACATGCTGTC TTAGTTCATG AAGGCCAAGC TAATGCAGGA 
CACTATTGGG CATATATTTT TGATCATCAC CAAGAAAGAT GGATGAAATA TAATGACATT 
TCTGTGACAA AATCAACATG GGAAGAGCTA GAGCGGGACT CTTTTGGTGG CTACAGAAAT 
GCCAGTGCAT ACTGTTTAAT GTATATTAAT GATAAGGAAC AATTCTTAAT AAGAGAAGAC 
TTTCACAAGG AAACTGGGCA GAAACTTGTC GGAATGGAAA CATTACCTAC TGATTTAAGG 
GAATTCGTCA AAGAAGATAA TCAGAGATTT GAAAAAGAGC TAGAGGATTG GGATCTACAA 
CTTGCCCAGA AAGCTCAGCA GGAAAAGATT TTAGCATCCC AGAAAATAAG GGAGCCAGCA 
CCTTCTGTAA TGACAGCACA AACAGAAGAA CCTGAATACC TAGAGCAGCC ATCCAGAACT 
GATCTCACCA AGTATTTGAA AGAAGAAACA ATGCGAGCGA TCACCAAGGC ATCGTCAGAG 
CATGAAGACA CAAATCCTGA AACTGTCTTA CAGCTGATCA TGATGACACC GAACATGCAA 
GGTATTATCA TGGCTATAGG TAAATCCAGG AGTGTATATG ACAGGTGTGG TCCTGAAGCA 
GGGTTCTTTA AGGCAATCAA ATTGGAGTAT GCAAGGTTGG TAAAGCTGGC TCAAGAAGAT 
ACTCCACCAG AAACAGATTA TCGATTGCAC CATGCAGTTG TCTATTTTAT CCAGAACCAA 
TCACCAAAGA AAATAATCGA GAGGACATTG TTGGAACAGT TTGGAAATCG CAATTTGAGT 
TTTGATGAGA GGTGTCGCAA CATAATGAAA ATTGCTCAAG CTAAACTTGA AATGATAAAG 
CCAGAAGAAG TAAACATGGA AGAATATGAG AAGTGGCACC AGGATTATAG GAACTTCAGA 
GATACAACAA TGTATCTCAT GATAGGCCTA GAACATTTTC AAAAAAAGAG TTACATAAAA 
GCCATACTGC ATCTTATCTG TGCTTATCAG AATAATAAGG AGCTCTTGTC TAAGGGCCCA 
TACCGAGGAC ATGATGAAGA ATTGATATCA CATTACAGAA GAGAATGTTT ACTAAAATTA 
AATGAGCATG CAGCAGAACT ATTTGAATCT GGAGATGATC AAGAAGTAAA CAATGGATTG 
ATTATTATGA ATGAGTTTAT TGTCCCAAGT TTGCCATTGT TACTGGTGGA TGAGATGGAA 
GAGAAAGATA TAGTTGCCGT AGAAGATATG AGAAATCGCT GGTGTTCATA TCTTGGACAA 
GAAATGGAAC CCAACCTCCA AGAAAAGCTG ACTGACTTCC TGCCAAAATT GCTTGATTGT 
TCTACAGAGA TCAAAGGTTT CAATGAGCTG CCAAAATTAC CTTCATATTC CACACATGAA 
CTGTGTGAAC GATTTGCCCG AATCATGTTG TCCCTCAGTC GAACTCCAGC TGATGGAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020831926.1
CDS	89..3331
Translation	MTVEQNVLQQSTSQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPYDRKRQDNCPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYSPPASAQDLPRNQKEHRNLPFMRELRHLFALLVGSKRKYVDPSRAVDILKDAFKSNGSQQQDVSEFTHKLLDWLEDAFQMKAEEERDGEKPKNPMLELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHKNREITRIKRDEIKRLKEYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTAEQQQGTPSELPSTSPTPRSFIHKPFTQSRIPPDLPMHPAPRHITEEELSVLEGCLHRWRTEVENDTRDLQESISRIHRTIEQMYSDKSMVQVPYRLHAVLVHEGQANAGHYWAYIFDHHQERWMKYNDISVTKSTWEELERDSFGGYRNASAYCLMYINDKEQFLIREDFHKETGQKLVGMETLPTDLREFVKEDNQRFEKELEDWDLQLAQKAQQEKILASQKIREPAPSVMTAQTEEPEYLEQPSRTDLTKYLKEETMRAITKASSEHEDTNPETVLQLIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHAVVYFIQNQSPKKIIERTLLEQFGNRNLSFDERCRNIMKIAQAKLEMIKPEEVNMEEYEKWHQDYRNFRDTTMYLMIGLEHFQKKSYIKAILHLICAYQNNKELLSKGPYRGHDEELISHYRRECLLKLNEHAAELFESGDDQEVNNGLIIMNEFIVPSLPLLLVDEMEEKDIVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKGFNELPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_020976267.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020976267.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCACCTCCC AGAAGCACCA GCAAACATTT 
TTGAATCAGC TGAGAGAAAT CACGGGCATC AATGATACAC AGATACTACA GCAAGCCTTG 
AAGGATAGCA ACGGAAACTT GGAATTAGCG GTGGCTTTCC TGACTGCAAA GAATGCCAAG 
ACACCTCAGC AAGAGGAAAC AACTTACTAT CAAACAGCCC TTCCTGGAAA TGATAGATAT 
ATCAGTGTGG GAAGCCAGGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GAAGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCTATTAGC AGAGTCCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCAAGTT TGAAGAGGAC GCATACAGAA GTCTGGAGTG ATTCTCCAAA CCCTTATGAT 
AGAAAAAGAC AGGATAATTG TCCAGTTGGG CTAAAGAATG TTGGAAATAC CTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT CTGGAATTTC GAAGATTAGT ACTGAATTAC 
AGTCCTCCTG CAAGTGCTCA AGATTTACCT CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGTTGCGGCA TCTGTTTGCA CTTCTTGTTG GTTCTAAAAG AAAATATGTT 
GATCCTTCAA GAGCAGTTGA TATTCTTAAA GATGCTTTCA AATCGAATGG CTCACAACAG 
CAAGATGTGA GTGAGTTCAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAAAGGGA TGGAGAGAAA CCAAAGAACC CAATGCTAGA ATTGTTCTAT 
GGAAGATTCC TAGCAGTGGG AGTGCTGGAA GGTAAAAAAT TTGAAAATAC AGAAATGTTT 
GGTCAGTATC CACTTCAAGT CAATGGTTTC AAAGATTTGC ATGAATGCCT AGAAGCTGCC 
ATGATTGAAG GAGAGATTGA GTCACTACAT TCAGAAAACT CAGCAAAATC TGGCCAAGAG 
CATTGGTTCA CAGAACTTCC ACCTGTATTA ACATTTGAAC TCTCAAGATT TGAATTTAAT 
CAGGCGTTGG GAAGACCAGA GAAAATTCAC AACAAGTTAG AATTCCCTCA AGTTTTATAT 
CTGGACAGAT ATATGCACAA AAACAGAGAA ATAACAAGAA TTAAACGAGA TGAGATCAAG 
AGGCTAAAAG AGTACCTTAC AGTATTACAA CAGAGATTAG AAAGATATTT AAGCTATGGT 
TCTGGCCCCA AACGATTCCC CTTGGTAGAT GTTCTACAGT ACGCACTGGA GTTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA GCAGAGCAAC AACAGGGAAC TCCTTCAGAA 
CTACCAAGCA CATCACCTAC ACCAAGGTCA TTTATACATA AGCCATTTAC ACAGTCACGA 
ATACCTCCAG ATTTGCCCAT GCATCCAGCA CCAAGACATA TAACAGAAGA AGAACTTTCT 
GTACTAGAAG GCTGTTTACA TCGCTGGAGG ACTGAAGTAG AAAATGACAC TAGAGATTTG 
CAGGAAAGCA TATCTAGAAT TCATCGTACA ATTGAACAAA TGTACTCTGA CAAGTCTATG 
GTACAAGTTC CTTATCGCTT ACATGCTGTC TTAGTTCATG AAGGCCAAGC TAATGCAGGA 
CACTATTGGG CATATATTTT TGATCATCAC CAAGAAAGAT GGATGAAATA TAATGACATT 
TCTGTGACAA AATCAACATG GGAAGAGCTA GAGCGGGACT CTTTTGGTGG CTACAGAAAT 
GCCAGTGCAT ACTGTTTAAT GTATATTAAT GATAAGGAAC AATTCTTAAT AAGAGAAGAC 
TTTCACAAGG AAACTGGGCA GAAACTTGTC GGAATGGAAA CATTACCTAC TGATTTAAGG 
GAATTCGTCA AAGAAGATAA TCAGAGATTT GAAAAAGAGC TAGAGGATTG GGATCTACAA 
CTTGCCCAGA AAGCTCAGCA GGAAAAGATT TTAGCATCCC AGAAAATAAG GGAGCCAGCA 
CCTTCTGTAA TGACAGCACA AACAGAAGAA CCTGAATACC TAGAGCAGCC ATCCAGAACT 
GATCTCACCA AGTATTTGAA AGAAGAAACA ATGCGAGCGA TCACCAAGGC ATCGTCAGAG 
CATGAAGACA CAAATCCTGA AACTGTCTTA CAGCTGATCA TGATGACACC GAACATGCAA 
GGTATTATCA TGGCTATAGG TAAATCCAGG AGTGTATATG ACAGGTGTGG TCCTGAAGCA 
GGGTTCTTTA AGGCAATCAA ATTGGAGTAT GCAAGGTTGG TAAAGCTGGC TCAAGAAGAT 
ACTCCACCAG AAACAGATTA TCGATTGCAC CATGCAGTTG TCTATTTTAT CCAGAACCAA 
TCACCAAAGA AAATAATCGA GAGGACATTG TTGGAACAGT TTGGAAATCG CAATTTGAGT 
TTTGATGAGA GGTGTCGCAA CATAATGAAA ATTGCTCAAG CTAAACTTGA AATGATAAAG 
CCAGAAGAAG TAAACATGGA AGAATATGAG AAGTGGCACC AGGATTATAG GAACTTCAGA 
GATACAACAA TGTATCTCAT GATAGGCCTA GAACATTTTC AAAAAAAGAG TTACATAAAA 
GCCATACTGC ATCTTATCTG TGCTTATCAG AATAATAAGG AGCTCTTGTC TAAGGGCCCA 
TACCGAGGAC ATGATGAAGA ATTGATATCA CATTACAGAA GAGAATGTTT ACTAAAATTA 
AATGAGCATG CAGCAGAACT ATTTGAATCT GGAGATGATC AAGAAGTAAA CAATGGATTG 
ATTATTATGA ATGAGTTTAT TGTCCCAAGT TTGCCATTGT TACTGGTGGA TGAGATGGAA 
GAGAAAGATA TAGTTGCCGT AGAAGATATG AGAAATCGCT GGTGTTCATA TCTTGGACAA 
GAAATGGAAC CCAACCTCCA AGAAAAGCTG ACTGACTTCC TGCCAAAATT GCTTGATTGT 
TCTACAGAGA TCAAAGGTTT CAATGAGCTG CCAAAATTAC CTTCATATTC CACACATGAA 
CTGTGTGAAC GATTTGCCCG AATCATGTTG TCCCTCAGTC GAACTCCAGC TGATGGAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_020976268.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020831927.1 Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OPh281497
Clone ID Related Accession (Same CDS sequence)	XM_020976268.1
Accession Version	XM_020976268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344237.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCACCTCCC AGAAGCACCA GCAAACATTT 
TTGAATCAGC TGAGAGAAAT CACGGGCATC AATGATACAC AGATACTACA GCAAGCCTTG 
AAGGATAGCA ACGGAAACTT GGAATTAGCG GTGGCTTTCC TGACTGCAAA GAATGCCAAG 
ACACCTCAGC AAGAGGAAAC AACTTACTAT CAAACAGCCC TTCCTGGAAA TGATAGATAT 
ATCAGTGTGG GAAGCCAGGC AGATACAAGA AAAAAACAGT ATAAGCCAAC CAGAACAAGA 
TGTGCCACAG TTGAGCAAAC ACATTATGGA TCAAAAGATA AGAGGGATAT GGATGTGATT 
GATCTCACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGAAGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCTAT TAGCAGAGTC 
CTTGAAGCCA GCATAGCAGA AAATAAAGCA AGTTTGAAGA GGACGCATAC AGAAGTCTGG 
AGTGATTCTC CAAACCCTTA TGATAGAAAA AGACAGGATA ATTGTCCAGT TGGGCTAAAG 
AATGTTGGAA ATACCTGCTG GTTTAGTGCT GTTATTCAGT CATTATTTAA TCTTCTGGAA 
TTTCGAAGAT TAGTACTGAA TTACAGTCCT CCTGCAAGTG CTCAAGATTT ACCTCGAAAC 
CAAAAGGAAC ATCGGAATTT GCCTTTTATG CGTGAGTTGC GGCATCTGTT TGCACTTCTT 
GTTGGTTCTA AAAGAAAATA TGTTGATCCT TCAAGAGCAG TTGATATTCT TAAAGATGCT 
TTCAAATCGA ATGGCTCACA ACAGCAAGAT GTGAGTGAGT TCACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAGGAAA GGGATGGAGA GAAACCAAAG 
AACCCAATGC TAGAATTGTT CTATGGAAGA TTCCTAGCAG TGGGAGTGCT GGAAGGTAAA 
AAATTTGAAA ATACAGAAAT GTTTGGTCAG TATCCACTTC AAGTCAATGG TTTCAAAGAT 
TTGCATGAAT GCCTAGAAGC TGCCATGATT GAAGGAGAGA TTGAGTCACT ACATTCAGAA 
AACTCAGCAA AATCTGGCCA AGAGCATTGG TTCACAGAAC TTCCACCTGT ATTAACATTT 
GAACTCTCAA GATTTGAATT TAATCAGGCG TTGGGAAGAC CAGAGAAAAT TCACAACAAG 
TTAGAATTCC CTCAAGTTTT ATATCTGGAC AGATATATGC ACAAAAACAG AGAAATAACA 
AGAATTAAAC GAGATGAGAT CAAGAGGCTA AAAGAGTACC TTACAGTATT ACAACAGAGA 
TTAGAAAGAT ATTTAAGCTA TGGTTCTGGC CCCAAACGAT TCCCCTTGGT AGATGTTCTA 
CAGTACGCAC TGGAGTTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCACCTAG TGGTTCCATA CCATCACAGA CATTACCAAG CACAGCAGAG 
CAACAACAGG GAACTCCTTC AGAACTACCA AGCACATCAC CTACACCAAG GTCATTTATA 
CATAAGCCAT TTACACAGTC ACGAATACCT CCAGATTTGC CCATGCATCC AGCACCAAGA 
CATATAACAG AAGAAGAACT TTCTGTACTA GAAGGCTGTT TACATCGCTG GAGGACTGAA 
GTAGAAAATG ACACTAGAGA TTTGCAGGAA AGCATATCTA GAATTCATCG TACAATTGAA 
CAAATGTACT CTGACAAGTC TATGGTACAA GTTCCTTATC GCTTACATGC TGTCTTAGTT 
CATGAAGGCC AAGCTAATGC AGGACACTAT TGGGCATATA TTTTTGATCA TCACCAAGAA 
AGATGGATGA AATATAATGA CATTTCTGTG ACAAAATCAA CATGGGAAGA GCTAGAGCGG 
GACTCTTTTG GTGGCTACAG AAATGCCAGT GCATACTGTT TAATGTATAT TAATGATAAG 
GAACAATTCT TAATAAGAGA AGACTTTCAC AAGGAAACTG GGCAGAAACT TGTCGGAATG 
GAAACATTAC CTACTGATTT AAGGGAATTC GTCAAAGAAG ATAATCAGAG ATTTGAAAAA 
GAGCTAGAGG ATTGGGATCT ACAACTTGCC CAGAAAGCTC AGCAGGAAAA GATTTTAGCA 
TCCCAGAAAA TAAGGGAGCC AGCACCTTCT GTAATGACAG CACAAACAGA AGAACCTGAA 
TACCTAGAGC AGCCATCCAG AACTGATCTC ACCAAGTATT TGAAAGAAGA AACAATGCGA 
GCGATCACCA AGGCATCGTC AGAGCATGAA GACACAAATC CTGAAACTGT CTTACAGCTG 
GCAATCAAAT TGGAGTATGC AAGGTTGGTA AAGCTGGCTC AAGAAGATAC TCCACCAGAA 
ACAGATTATC GATTGCACCA TGCAGTTGTC TATTTTATCC AGAACCAATC ACCAAAGAAA 
ATAATCGAGA GGACATTGTT GGAACAGTTT GGAAATCGCA ATTTGAGTTT TGATGAGAGG 
TGTCGCAACA TAATGAAAAT TGCTCAAGCT AAACTTGAAA TGATAAAGCC AGAAGAAGTA 
AACATGGAAG AATATGAGAA GTGGCACCAG GATTATAGGA ACTTCAGAGA TACAACAATG 
TATCTCATGA TAGGCCTAGA ACATTTTCAA AAAAAGAGTT ACATAAAAGC CATACTGCAT 
CTTATCTGTG CTTATCAGAA TAATAAGGAG CTCTTGTCTA AGGGCCCATA CCGAGGACAT 
GATGAAGAAT TGATATCACA TTACAGAAGA GAATGTTTAC TAAAATTAAA TGAGCATGCA 
GCAGAACTAT TTGAATCTGG AGATGATCAA GAAGTAAACA ATGGATTGAT TATTATGAAT 
GAGTTTATTG TCCCAAGTTT GCCATTGTTA CTGGTGGATG AGATGGAAGA GAAAGATATA 
GTTGCCGTAG AAGATATGAG AAATCGCTGG TGTTCATATC TTGGACAAGA AATGGAACCC 
AACCTCCAAG AAAAGCTGAC TGACTTCCTG CCAAAATTGC TTGATTGTTC TACAGAGATC 
AAAGGTTTCA ATGAGCTGCC AAAATTACCT TCATATTCCA CACATGAACT GTGTGAACGA 
TTTGCCCGAA TCATGTTGTC CCTCAGTCGA ACTCCAGCTG ATGGAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020831927.1
CDS	89..3319
Translation	MTVEQNVLQQSTSQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKQYKPTRTRCATVEQTHYGSKDKRDMDVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPYDRKRQDNCPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYSPPASAQDLPRNQKEHRNLPFMRELRHLFALLVGSKRKYVDPSRAVDILKDAFKSNGSQQQDVSEFTHKLLDWLEDAFQMKAEEERDGEKPKNPMLELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHKNREITRIKRDEIKRLKEYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTAEQQQGTPSELPSTSPTPRSFIHKPFTQSRIPPDLPMHPAPRHITEEELSVLEGCLHRWRTEVENDTRDLQESISRIHRTIEQMYSDKSMVQVPYRLHAVLVHEGQANAGHYWAYIFDHHQERWMKYNDISVTKSTWEELERDSFGGYRNASAYCLMYINDKEQFLIREDFHKETGQKLVGMETLPTDLREFVKEDNQRFEKELEDWDLQLAQKAQQEKILASQKIREPAPSVMTAQTEEPEYLEQPSRTDLTKYLKEETMRAITKASSEHEDTNPETVLQLAIKLEYARLVKLAQEDTPPETDYRLHHAVVYFIQNQSPKKIIERTLLEQFGNRNLSFDERCRNIMKIAQAKLEMIKPEEVNMEEYEKWHQDYRNFRDTTMYLMIGLEHFQKKSYIKAILHLICAYQNNKELLSKGPYRGHDEELISHYRRECLLKLNEHAAELFESGDDQEVNNGLIIMNEFIVPSLPLLLVDEMEEKDIVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKGFNELPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_020976268.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020976268.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCACCTCCC AGAAGCACCA GCAAACATTT 
TTGAATCAGC TGAGAGAAAT CACGGGCATC AATGATACAC AGATACTACA GCAAGCCTTG 
AAGGATAGCA ACGGAAACTT GGAATTAGCG GTGGCTTTCC TGACTGCAAA GAATGCCAAG 
ACACCTCAGC AAGAGGAAAC AACTTACTAT CAAACAGCCC TTCCTGGAAA TGATAGATAT 
ATCAGTGTGG GAAGCCAGGC AGATACAAGA AAAAAACAGT ATAAGCCAAC CAGAACAAGA 
TGTGCCACAG TTGAGCAAAC ACATTATGGA TCAAAAGATA AGAGGGATAT GGATGTGATT 
GATCTCACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGAAGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCTAT TAGCAGAGTC 
CTTGAAGCCA GCATAGCAGA AAATAAAGCA AGTTTGAAGA GGACGCATAC AGAAGTCTGG 
AGTGATTCTC CAAACCCTTA TGATAGAAAA AGACAGGATA ATTGTCCAGT TGGGCTAAAG 
AATGTTGGAA ATACCTGCTG GTTTAGTGCT GTTATTCAGT CATTATTTAA TCTTCTGGAA 
TTTCGAAGAT TAGTACTGAA TTACAGTCCT CCTGCAAGTG CTCAAGATTT ACCTCGAAAC 
CAAAAGGAAC ATCGGAATTT GCCTTTTATG CGTGAGTTGC GGCATCTGTT TGCACTTCTT 
GTTGGTTCTA AAAGAAAATA TGTTGATCCT TCAAGAGCAG TTGATATTCT TAAAGATGCT 
TTCAAATCGA ATGGCTCACA ACAGCAAGAT GTGAGTGAGT TCACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAGGAAA GGGATGGAGA GAAACCAAAG 
AACCCAATGC TAGAATTGTT CTATGGAAGA TTCCTAGCAG TGGGAGTGCT GGAAGGTAAA 
AAATTTGAAA ATACAGAAAT GTTTGGTCAG TATCCACTTC AAGTCAATGG TTTCAAAGAT 
TTGCATGAAT GCCTAGAAGC TGCCATGATT GAAGGAGAGA TTGAGTCACT ACATTCAGAA 
AACTCAGCAA AATCTGGCCA AGAGCATTGG TTCACAGAAC TTCCACCTGT ATTAACATTT 
GAACTCTCAA GATTTGAATT TAATCAGGCG TTGGGAAGAC CAGAGAAAAT TCACAACAAG 
TTAGAATTCC CTCAAGTTTT ATATCTGGAC AGATATATGC ACAAAAACAG AGAAATAACA 
AGAATTAAAC GAGATGAGAT CAAGAGGCTA AAAGAGTACC TTACAGTATT ACAACAGAGA 
TTAGAAAGAT ATTTAAGCTA TGGTTCTGGC CCCAAACGAT TCCCCTTGGT AGATGTTCTA 
CAGTACGCAC TGGAGTTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCACCTAG TGGTTCCATA CCATCACAGA CATTACCAAG CACAGCAGAG 
CAACAACAGG GAACTCCTTC AGAACTACCA AGCACATCAC CTACACCAAG GTCATTTATA 
CATAAGCCAT TTACACAGTC ACGAATACCT CCAGATTTGC CCATGCATCC AGCACCAAGA 
CATATAACAG AAGAAGAACT TTCTGTACTA GAAGGCTGTT TACATCGCTG GAGGACTGAA 
GTAGAAAATG ACACTAGAGA TTTGCAGGAA AGCATATCTA GAATTCATCG TACAATTGAA 
CAAATGTACT CTGACAAGTC TATGGTACAA GTTCCTTATC GCTTACATGC TGTCTTAGTT 
CATGAAGGCC AAGCTAATGC AGGACACTAT TGGGCATATA TTTTTGATCA TCACCAAGAA 
AGATGGATGA AATATAATGA CATTTCTGTG ACAAAATCAA CATGGGAAGA GCTAGAGCGG 
GACTCTTTTG GTGGCTACAG AAATGCCAGT GCATACTGTT TAATGTATAT TAATGATAAG 
GAACAATTCT TAATAAGAGA AGACTTTCAC AAGGAAACTG GGCAGAAACT TGTCGGAATG 
GAAACATTAC CTACTGATTT AAGGGAATTC GTCAAAGAAG ATAATCAGAG ATTTGAAAAA 
GAGCTAGAGG ATTGGGATCT ACAACTTGCC CAGAAAGCTC AGCAGGAAAA GATTTTAGCA 
TCCCAGAAAA TAAGGGAGCC AGCACCTTCT GTAATGACAG CACAAACAGA AGAACCTGAA 
TACCTAGAGC AGCCATCCAG AACTGATCTC ACCAAGTATT TGAAAGAAGA AACAATGCGA 
GCGATCACCA AGGCATCGTC AGAGCATGAA GACACAAATC CTGAAACTGT CTTACAGCTG 
GCAATCAAAT TGGAGTATGC AAGGTTGGTA AAGCTGGCTC AAGAAGATAC TCCACCAGAA 
ACAGATTATC GATTGCACCA TGCAGTTGTC TATTTTATCC AGAACCAATC ACCAAAGAAA 
ATAATCGAGA GGACATTGTT GGAACAGTTT GGAAATCGCA ATTTGAGTTT TGATGAGAGG 
TGTCGCAACA TAATGAAAAT TGCTCAAGCT AAACTTGAAA TGATAAAGCC AGAAGAAGTA 
AACATGGAAG AATATGAGAA GTGGCACCAG GATTATAGGA ACTTCAGAGA TACAACAATG 
TATCTCATGA TAGGCCTAGA ACATTTTCAA AAAAAGAGTT ACATAAAAGC CATACTGCAT 
CTTATCTGTG CTTATCAGAA TAATAAGGAG CTCTTGTCTA AGGGCCCATA CCGAGGACAT 
GATGAAGAAT TGATATCACA TTACAGAAGA GAATGTTTAC TAAAATTAAA TGAGCATGCA 
GCAGAACTAT TTGAATCTGG AGATGATCAA GAAGTAAACA ATGGATTGAT TATTATGAAT 
GAGTTTATTG TCCCAAGTTT GCCATTGTTA CTGGTGGATG AGATGGAAGA GAAAGATATA 
GTTGCCGTAG AAGATATGAG AAATCGCTGG TGTTCATATC TTGGACAAGA AATGGAACCC 
AACCTCCAAG AAAAGCTGAC TGACTTCCTG CCAAAATTGC TTGATTGTTC TACAGAGATC 
AAAGGTTTCA ATGAGCTGCC AAAATTACCT TCATATTCCA CACATGAACT GTGTGAACGA 
TTTGCCCGAA TCATGTTGTC CCTCAGTCGA ACTCCAGCTG ATGGAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_020976269.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020831928.1 Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X8, mRNA.

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CloneID	OPh281498
Clone ID Related Accession (Same CDS sequence)	XM_020976269.1
Accession Version	XM_020976269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018344237.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCACCTCCC AGAAGCACCA GCAAACATTT 
TTGAATCAGC TGAGAGAAAT CACGGGCATC AATGATACAC AGATACTACA GCAAGCCTTG 
AAGGATAGCA ACGGAAACTT GGAATTAGCG GTGGCTTTCC TGACTGCAAA GAATGCCAAG 
ACACCTCAGC AAGAGGAAAC AACTTACTAT CAAACAGCCC TTCCTGGAAA TGATAGATAT 
ATCAGTGTGG GAAGCCAGGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GAAGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCTATTAGC AGAGTCCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCAAGTT TGAAGAGGAC GCATACAGAA GTCTGGAGTG ATTCTCCAAA CCCTTATGAT 
AGAAAAAGAC AGGATAATTG TCCAGTTGGG CTAAAGAATG TTGGAAATAC CTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT CTGGAATTTC GAAGATTAGT ACTGAATTAC 
AGTCCTCCTG CAAGTGCTCA AGATTTACCT CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGTTGCGGCA TCTGTTTGCA CTTCTTGTTG GTTCTAAAAG AAAATATGTT 
GATCCTTCAA GAGCAGTTGA TATTCTTAAA GATGCTTTCA AATCGAATGG CTCACAACAG 
CAAGATGTGA GTGAGTTCAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAAAGGGA TGGAGAGAAA CCAAAGAACC CAATGCTAGA ATTGTTCTAT 
GGAAGATTCC TAGCAGTGGG AGTGCTGGAA GGTAAAAAAT TTGAAAATAC AGAAATGTTT 
GGTCAGTATC CACTTCAAGT CAATGGTTTC AAAGATTTGC ATGAATGCCT AGAAGCTGCC 
ATGATTGAAG GAGAGATTGA GTCACTACAT TCAGAAAACT CAGCAAAATC TGGCCAAGAG 
CATTGGTTCA CAGAACTTCC ACCTGTATTA ACATTTGAAC TCTCAAGATT TGAATTTAAT 
CAGGCGTTGG GAAGACCAGA GAAAATTCAC AACAAGTTAG AATTCCCTCA AGTTTTATAT 
CTGGACAGAT ATATGCACAA AAACAGAGAA ATAACAAGAA TTAAACGAGA TGAGATCAAG 
AGGCTAAAAG AGTACCTTAC AGTATTACAA CAGAGATTAG AAAGATATTT AAGCTATGGT 
TCTGGCCCCA AACGATTCCC CTTGGTAGAT GTTCTACAGT ACGCACTGGA GTTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA GCAGAGCAAC AACAGGGAAC TCCTTCAGAA 
CTACCAAGCA CATCACCTAC ACCAAGGTCA TTTATACATA AGCCATTTAC ACAGTCACGA 
ATACCTCCAG ATTTGCCCAT GCATCCAGCA CCAAGACATA TAACAGAAGA AGAACTTTCT 
GTACTAGAAG GCTGTTTACA TCGCTGGAGG ACTGAAGTAG AAAATGACAC TAGAGATTTG 
CAGGAAAGCA TATCTAGAAT TCATCGTACA ATTGAACAAA TGTACTCTGA CAAGTCTATG 
GTACAAGTTC CTTATCGCTT ACATGCTGTC TTAGTTCATG AAGGCCAAGC TAATGCAGGA 
CACTATTGGG CATATATTTT TGATCATCAC CAAGAAAGAT GGATGAAATA TAATGACATT 
TCTGTGACAA AATCAACATG GGAAGAGCTA GAGCGGGACT CTTTTGGTGG CTACAGAAAT 
GCCAGTGCAT ACTGTTTAAT GTATATTAAT GATAAGGAAC AATTCTTAAT AAGAGAAGAC 
TTTCACAAGG AAACTGGGCA GAAACTTGTC GGAATGGAAA CATTACCTAC TGATTTAAGG 
GAATTCGTCA AAGAAGATAA TCAGAGATTT GAAAAAGAGC TAGAGGATTG GGATCTACAA 
CTTGCCCAGA AAGCTCAGCA GGAAAAGATT TTAGCATCCC AGAAAATAAG GGAGCCAGCA 
CCTTCTGTAA TGACAGCACA AACAGAAGAA CCTGAATACC TAGAGCAGCC ATCCAGAACT 
GATCTCACCA AGTATTTGAA AGAAGAAACA ATGCGAGCGA TCACCAAGGC ATCGTCAGAG 
CATGAAGACA CAAATCCTGA AACTGTCTTA CAGCTGGCAA TCAAATTGGA GTATGCAAGG 
TTGGTAAAGC TGGCTCAAGA AGATACTCCA CCAGAAACAG ATTATCGATT GCACCATGCA 
GTTGTCTATT TTATCCAGAA CCAATCACCA AAGAAAATAA TCGAGAGGAC ATTGTTGGAA 
CAGTTTGGAA ATCGCAATTT GAGTTTTGAT GAGAGGTGTC GCAACATAAT GAAAATTGCT 
CAAGCTAAAC TTGAAATGAT AAAGCCAGAA GAAGTAAACA TGGAAGAATA TGAGAAGTGG 
CACCAGGATT ATAGGAACTT CAGAGATACA ACAATGTATC TCATGATAGG CCTAGAACAT 
TTTCAAAAAA AGAGTTACAT AAAAGCCATA CTGCATCTTA TCTGTGCTTA TCAGAATAAT 
AAGGAGCTCT TGTCTAAGGG CCCATACCGA GGACATGATG AAGAATTGAT ATCACATTAC 
AGAAGAGAAT GTTTACTAAA ATTAAATGAG CATGCAGCAG AACTATTTGA ATCTGGAGAT 
GATCAAGAAG TAAACAATGG ATTGATTATT ATGAATGAGT TTATTGTCCC AAGTTTGCCA 
TTGTTACTGG TGGATGAGAT GGAAGAGAAA GATATAGTTG CCGTAGAAGA TATGAGAAAT 
CGCTGGTGTT CATATCTTGG ACAAGAAATG GAACCCAACC TCCAAGAAAA GCTGACTGAC 
TTCCTGCCAA AATTGCTTGA TTGTTCTACA GAGATCAAAG GTTTCAATGA GCTGCCAAAA 
TTACCTTCAT ATTCCACACA TGAACTGTGT GAACGATTTG CCCGAATCAT GTTGTCCCTC 
AGTCGAACTC CAGCTGATGG AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020831928.1
CDS	89..3235
Translation	MTVEQNVLQQSTSQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPYDRKRQDNCPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYSPPASAQDLPRNQKEHRNLPFMRELRHLFALLVGSKRKYVDPSRAVDILKDAFKSNGSQQQDVSEFTHKLLDWLEDAFQMKAEEERDGEKPKNPMLELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHKNREITRIKRDEIKRLKEYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTAEQQQGTPSELPSTSPTPRSFIHKPFTQSRIPPDLPMHPAPRHITEEELSVLEGCLHRWRTEVENDTRDLQESISRIHRTIEQMYSDKSMVQVPYRLHAVLVHEGQANAGHYWAYIFDHHQERWMKYNDISVTKSTWEELERDSFGGYRNASAYCLMYINDKEQFLIREDFHKETGQKLVGMETLPTDLREFVKEDNQRFEKELEDWDLQLAQKAQQEKILASQKIREPAPSVMTAQTEEPEYLEQPSRTDLTKYLKEETMRAITKASSEHEDTNPETVLQLAIKLEYARLVKLAQEDTPPETDYRLHHAVVYFIQNQSPKKIIERTLLEQFGNRNLSFDERCRNIMKIAQAKLEMIKPEEVNMEEYEKWHQDYRNFRDTTMYLMIGLEHFQKKSYIKAILHLICAYQNNKELLSKGPYRGHDEELISHYRRECLLKLNEHAAELFESGDDQEVNNGLIIMNEFIVPSLPLLLVDEMEEKDIVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKGFNELPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_020976269.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus ubiquitin specific peptidase 25 (USP25), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020976269.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCACCTCCC AGAAGCACCA GCAAACATTT 
TTGAATCAGC TGAGAGAAAT CACGGGCATC AATGATACAC AGATACTACA GCAAGCCTTG 
AAGGATAGCA ACGGAAACTT GGAATTAGCG GTGGCTTTCC TGACTGCAAA GAATGCCAAG 
ACACCTCAGC AAGAGGAAAC AACTTACTAT CAAACAGCCC TTCCTGGAAA TGATAGATAT 
ATCAGTGTGG GAAGCCAGGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GAAGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCTATTAGC AGAGTCCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCAAGTT TGAAGAGGAC GCATACAGAA GTCTGGAGTG ATTCTCCAAA CCCTTATGAT 
AGAAAAAGAC AGGATAATTG TCCAGTTGGG CTAAAGAATG TTGGAAATAC CTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT CTGGAATTTC GAAGATTAGT ACTGAATTAC 
AGTCCTCCTG CAAGTGCTCA AGATTTACCT CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGTTGCGGCA TCTGTTTGCA CTTCTTGTTG GTTCTAAAAG AAAATATGTT 
GATCCTTCAA GAGCAGTTGA TATTCTTAAA GATGCTTTCA AATCGAATGG CTCACAACAG 
CAAGATGTGA GTGAGTTCAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAAAGGGA TGGAGAGAAA CCAAAGAACC CAATGCTAGA ATTGTTCTAT 
GGAAGATTCC TAGCAGTGGG AGTGCTGGAA GGTAAAAAAT TTGAAAATAC AGAAATGTTT 
GGTCAGTATC CACTTCAAGT CAATGGTTTC AAAGATTTGC ATGAATGCCT AGAAGCTGCC 
ATGATTGAAG GAGAGATTGA GTCACTACAT TCAGAAAACT CAGCAAAATC TGGCCAAGAG 
CATTGGTTCA CAGAACTTCC ACCTGTATTA ACATTTGAAC TCTCAAGATT TGAATTTAAT 
CAGGCGTTGG GAAGACCAGA GAAAATTCAC AACAAGTTAG AATTCCCTCA AGTTTTATAT 
CTGGACAGAT ATATGCACAA AAACAGAGAA ATAACAAGAA TTAAACGAGA TGAGATCAAG 
AGGCTAAAAG AGTACCTTAC AGTATTACAA CAGAGATTAG AAAGATATTT AAGCTATGGT 
TCTGGCCCCA AACGATTCCC CTTGGTAGAT GTTCTACAGT ACGCACTGGA GTTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA GCAGAGCAAC AACAGGGAAC TCCTTCAGAA 
CTACCAAGCA CATCACCTAC ACCAAGGTCA TTTATACATA AGCCATTTAC ACAGTCACGA 
ATACCTCCAG ATTTGCCCAT GCATCCAGCA CCAAGACATA TAACAGAAGA AGAACTTTCT 
GTACTAGAAG GCTGTTTACA TCGCTGGAGG ACTGAAGTAG AAAATGACAC TAGAGATTTG 
CAGGAAAGCA TATCTAGAAT TCATCGTACA ATTGAACAAA TGTACTCTGA CAAGTCTATG 
GTACAAGTTC CTTATCGCTT ACATGCTGTC TTAGTTCATG AAGGCCAAGC TAATGCAGGA 
CACTATTGGG CATATATTTT TGATCATCAC CAAGAAAGAT GGATGAAATA TAATGACATT 
TCTGTGACAA AATCAACATG GGAAGAGCTA GAGCGGGACT CTTTTGGTGG CTACAGAAAT 
GCCAGTGCAT ACTGTTTAAT GTATATTAAT GATAAGGAAC AATTCTTAAT AAGAGAAGAC 
TTTCACAAGG AAACTGGGCA GAAACTTGTC GGAATGGAAA CATTACCTAC TGATTTAAGG 
GAATTCGTCA AAGAAGATAA TCAGAGATTT GAAAAAGAGC TAGAGGATTG GGATCTACAA 
CTTGCCCAGA AAGCTCAGCA GGAAAAGATT TTAGCATCCC AGAAAATAAG GGAGCCAGCA 
CCTTCTGTAA TGACAGCACA AACAGAAGAA CCTGAATACC TAGAGCAGCC ATCCAGAACT 
GATCTCACCA AGTATTTGAA AGAAGAAACA ATGCGAGCGA TCACCAAGGC ATCGTCAGAG 
CATGAAGACA CAAATCCTGA AACTGTCTTA CAGCTGGCAA TCAAATTGGA GTATGCAAGG 
TTGGTAAAGC TGGCTCAAGA AGATACTCCA CCAGAAACAG ATTATCGATT GCACCATGCA 
GTTGTCTATT TTATCCAGAA CCAATCACCA AAGAAAATAA TCGAGAGGAC ATTGTTGGAA 
CAGTTTGGAA ATCGCAATTT GAGTTTTGAT GAGAGGTGTC GCAACATAAT GAAAATTGCT 
CAAGCTAAAC TTGAAATGAT AAAGCCAGAA GAAGTAAACA TGGAAGAATA TGAGAAGTGG 
CACCAGGATT ATAGGAACTT CAGAGATACA ACAATGTATC TCATGATAGG CCTAGAACAT 
TTTCAAAAAA AGAGTTACAT AAAAGCCATA CTGCATCTTA TCTGTGCTTA TCAGAATAAT 
AAGGAGCTCT TGTCTAAGGG CCCATACCGA GGACATGATG AAGAATTGAT ATCACATTAC 
AGAAGAGAAT GTTTACTAAA ATTAAATGAG CATGCAGCAG AACTATTTGA ATCTGGAGAT 
GATCAAGAAG TAAACAATGG ATTGATTATT ATGAATGAGT TTATTGTCCC AAGTTTGCCA 
TTGTTACTGG TGGATGAGAT GGAAGAGAAA GATATAGTTG CCGTAGAAGA TATGAGAAAT 
CGCTGGTGTT CATATCTTGG ACAAGAAATG GAACCCAACC TCCAAGAAAA GCTGACTGAC 
TTCCTGCCAA AATTGCTTGA TTGTTCTACA GAGATCAAAG GTTTCAATGA GCTGCCAAAA 
TTACCTTCAT ATTCCACACA TGAACTGTGT GAACGATTTG CCCGAATCAT GTTGTCCCTC 
AGTCGAACTC CAGCTGATGG AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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USP25 cDNA ORF clone, Phascolarctos cinereus(koala)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム