crim1 cDNA ORF clone,

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gRNAs

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OYe107340	XM_028565194.1 Latest version!	Perca flavescens cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_028565194.1 ) cDNA ORF clone, -> XP_028420995.1 Perca flavescens cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

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CloneID	OYe107340
Clone ID Related Accession (Same CDS sequence)	XM_028565194.1
Accession Version	XM_028565194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-31
Organism	Perca flavescens(yellow perch)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041350.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Perca flavescens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCA AGTGCTCCGT TGTGTTATTG AATGTTTTTT TTTTGGTGCG GGTGCTCTCG 
GCTAATGAGT GCACGCCGTG TACTCTCCGG ACTTGTCCTG TCAAAGCCCC GCACGGCTGC 
GAAGGTGGCC GGACCTTGGC CAGGGATCCG TGCGGATGCT GTGACCAGTG TGCCCGGCTG 
GAGTGGGAGC CATGCGGCGG ACAGGACTGG GCGCATGGCT ACTGCGCCCT GGGACTGACC 
TGCGCCTCTG TTAACAAAAC AGGAGCAGCC ACAATCCCTG AAACTGGAGT ATGTAAAGTG 
CTCCCTGATC ATCCAGAGGC AGGCTTGGAG GATGAGCGCT GTCCCTTGAT GACAGGCTGC 
GATAGAGCTG GAGGTCAGTG TGTGTGCGAT GCCCGTCACT CCTGCTTGGG CTCTTTCACC 
TACCCAGACC AAGAAACCTG CATGAAAGCC AGCAAGTCAG ATGTTCGCAG GCACGAGCAC 
CTGGAAAGAC ACAAAGAGAA GTATGTGGGA CCATCCAACC GGGCCTGCAT GTTCTCTGGC 
TGTAACCTGA CCACCGAGGG CTGTGTGTGT GAGTCTCAGA GCTGCCACCA CCACTTCGCC 
TACATCAACC GCAGCCAGTG CCAGGAAGCC GCAGAGGAGC TGAGGTGTGC TGGGCTGACG 
TGTCCAACTC TGCAGGTGCC CTCCTGCCCC AAGGGTTCAG TCCTGACCAA GAGTTACACG 
CCCCCTACTG GTTGCTGCCC CTCCATACCG CCCCAGTGCA CTTGCGACCT CCGTGCCTGC 
CGCAAGCCCC AGTGCCCGAA CGGACAGCGC GCCGTGCCAC TCAGCCAGGC CAGCGGTCAG 
CCAGGAGACT GCTGTGATGT CTTTGAGTGC CAGAAAGTCT CTCCCAAGTG TGTCCACAAT 
GGGAAAGAGT TTTCCGAGGG CGAGGTGTAC CGCATGGACC CCTGCTGGCT GTGTCAGTGC 
CGCGGAGGAA TCTCCTTCTG CTCCAAGGCG GAGTGCGCTG AGCTGGACTG TGAAAACTTC 
TACATTCCTG AGGGCGAGTG TTGCCCTGTC TGCATAGATG TGGAGTTACT ATCAATGGAG 
AGCACTAAGG CCAGTTGCTG GGTGAATAAT AAGCTGCGGG CCCATGAGGA GCAGTGGAAA 
GAGGACGATT GCACCTTCTG CCAGTGTGTG GATGGAGAAC CGCACTGCAC GGCCATGGCC 
TGCAAACAGA GCTGCCAATA CCCTGTCAAG ATACCGGGAG AGTGCTGTCC CTTCTGTGAA 
GAGCCTTCTT ACGAAACTGT TAGCCCCATG CTATGCCCTC CTCTGGTAAA CTGCTCCCTG 
TCAGGGAATG ACTGCCTCTA CGGCTTCCAA CAAGACAGAA ATGGATGTCT GCTCTGCCAC 
TGTCTCAACA ATGACTCCTG CCCTGACCTG GGAAAATACT GTTCACTGCA ATGCCCAATG 
GGCTACAAGA AGGATGATTT TGGCTGTGAT GTGTGCGAGT GCAGCATGCC CAGGTGCCGA 
CCTTTGACCT GCACTAAGAC CTGCCCCTAT GGATATGTGA GAAACAAGCA TGGCTGTGAG 
ATGTGTCGCT GTGTCAAGTG TCCTCCCTTC ACCTGTGACA AGCACTGCAG CGACGGCTAT 
CGGCAGAACA GGAAGGGCTG CTCCATCTGC ATGTGTAAAG AAAGCAGCCA TGTCCCAAGC 
ACCACTCCCC TGGCCCCGAT GCCCAGCTAT TGCCTCACCT CCAATGGGCA CCGGTATGAG 
GAAGGCGACG GCTGGCACGA CGGTTGTCGT GACTGCTACT GCCACTCCGG ACGGGAGATG 
TGTGTGCTCA TTTCCTGCCC CGTACCCAGC TGTTCTCACC CAGTTGTAAG GCCTGACCAG 
TGTTGTCCTA CATGTGAGGA TGAGTCAGGC AGCGGTCTGC CAGAGGGGAT GGACATGGTG 
GTGTGCAGAG CACCTGGGGG AGAATTTTAT GTGGAGGGGG AGACCTGGAA CCTGGACGAG 
TGCACAAGGT GCACCTGCAG GAAAGGACGT GTCCTGTGCG ACACTGAAGT GTGTCCACCA 
GCTCTCTGCC AGACACCAAT CAGAAACAAG AACACCTGTT GCCACGTATG CCCAGAGGAG 
ACACTGAGCC CCTTGCTGCC AGTGAACAAC AGCCAGCAGG AGTACTGCAT AACCAGCGAT 
GGGGACGTGC TACTGGCCGG GGACTCCTGG AAGGCCAACG CCTGCACCAG CTGCACCTGC 
AACAATGGCA CCATTCAGTG TTTCTCTCAG CGCTGTCCGG CTGCCAACTG CAGGGTGCCC 
GTCTTACGCA AGGGCCAGTG CTGCCCACAC TGTCTCGAAA TTACAACGTC AGTACCGGTG 
ATGGTGTCTA CCGACGCCCC CAAGACAATG GTCCCTATTA CAGTGGAGAG TGCAGTGTGG 
ATGACCACAA TCACTGTACC ACCCATCATT GCTGAGACAG ATGAGCCAGG TTTGCGTGAG 
ATTTACCCCA GCCAAACAGA GATGGCCATC ATCTACCAAT CAGCAGCCTG GATCCTGGCA 
GGTCTCCTTC TGGCTATCAT TATCTTCCTC GTCGTGGCAT TAATAATCAA CAAGAAAAAG 
AAGTGGGTGC AGATGTCCTG CTACAGTGCA CCAAAGAAGA CGGTGATCCT GAAGAAGCAC 
GTAAACAAGA ACTCAGTGGT CTACATGGAG CCCTCCAAGG AGAACAAATT TCAGAATGTG 
AAGAAGGACT GCGGCATCAT CCATTTCTCT CCAGAAATGA GCTCCACCTG CGCAGACAAG 
ATGACCATCC CCAGGGCCAA GCTGAGCATG GGCAACGACT GGACAAAGCG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028420995.1
CDS	434..3247
Translation	MFSKCSVVLLNVFFLVRVLSANECTPCTLRTCPVKAPHGCEGGRTLARDPCGCCDQCARLEWEPCGGQDWAHGYCALGLTCASVNKTGAATIPETGVCKVLPDHPEAGLEDERCPLMTGCDRAGGQCVCDARHSCLGSFTYPDQETCMKASKSDVRRHEHLERHKEKYVGPSNRACMFSGCNLTTEGCVCESQSCHHHFAYINRSQCQEAAEELRCAGLTCPTLQVPSCPKGSVLTKSYTPPTGCCPSIPPQCTCDLRACRKPQCPNGQRAVPLSQASGQPGDCCDVFECQKVSPKCVHNGKEFSEGEVYRMDPCWLCQCRGGISFCSKAECAELDCENFYIPEGECCPVCIDVELLSMESTKASCWVNNKLRAHEEQWKEDDCTFCQCVDGEPHCTAMACKQSCQYPVKIPGECCPFCEEPSYETVSPMLCPPLVNCSLSGNDCLYGFQQDRNGCLLCHCLNNDSCPDLGKYCSLQCPMGYKKDDFGCDVCECSMPRCRPLTCTKTCPYGYVRNKHGCEMCRCVKCPPFTCDKHCSDGYRQNRKGCSICMCKESSHVPSTTPLAPMPSYCLTSNGHRYEEGDGWHDGCRDCYCHSGREMCVLISCPVPSCSHPVVRPDQCCPTCEDESGSGLPEGMDMVVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEVCPPALCQTPIRNKNTCCHVCPEETLSPLLPVNNSQQEYCITSDGDVLLAGDSWKANACTSCTCNNGTIQCFSQRCPAANCRVPVLRKGQCCPHCLEITTSVPVMVSTDAPKTMVPITVESAVWMTTITVPPIIAETDEPGLREIYPSQTEMAIIYQSAAWILAGLLLAIIIFLVVALIINKKKKWVQMSCYSAPKKTVILKKHVNKNSVVYMEPSKENKFQNVKKDCGIIHFSPEMSSTCADKMTIPRAKLSMGNDWTKR

Target ORF information:

RefSeq Version	XM_028565194.1
Organism	Perca flavescens(yellow perch)
Definition	Perca flavescens cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028565194.1

ORF Insert Sequence:

ATGTTCAGCA AGTGCTCCGT TGTGTTATTG AATGTTTTTT TTTTGGTGCG GGTGCTCTCG 
GCTAATGAGT GCACGCCGTG TACTCTCCGG ACTTGTCCTG TCAAAGCCCC GCACGGCTGC 
GAAGGTGGCC GGACCTTGGC CAGGGATCCG TGCGGATGCT GTGACCAGTG TGCCCGGCTG 
GAGTGGGAGC CATGCGGCGG ACAGGACTGG GCGCATGGCT ACTGCGCCCT GGGACTGACC 
TGCGCCTCTG TTAACAAAAC AGGAGCAGCC ACAATCCCTG AAACTGGAGT ATGTAAAGTG 
CTCCCTGATC ATCCAGAGGC AGGCTTGGAG GATGAGCGCT GTCCCTTGAT GACAGGCTGC 
GATAGAGCTG GAGGTCAGTG TGTGTGCGAT GCCCGTCACT CCTGCTTGGG CTCTTTCACC 
TACCCAGACC AAGAAACCTG CATGAAAGCC AGCAAGTCAG ATGTTCGCAG GCACGAGCAC 
CTGGAAAGAC ACAAAGAGAA GTATGTGGGA CCATCCAACC GGGCCTGCAT GTTCTCTGGC 
TGTAACCTGA CCACCGAGGG CTGTGTGTGT GAGTCTCAGA GCTGCCACCA CCACTTCGCC 
TACATCAACC GCAGCCAGTG CCAGGAAGCC GCAGAGGAGC TGAGGTGTGC TGGGCTGACG 
TGTCCAACTC TGCAGGTGCC CTCCTGCCCC AAGGGTTCAG TCCTGACCAA GAGTTACACG 
CCCCCTACTG GTTGCTGCCC CTCCATACCG CCCCAGTGCA CTTGCGACCT CCGTGCCTGC 
CGCAAGCCCC AGTGCCCGAA CGGACAGCGC GCCGTGCCAC TCAGCCAGGC CAGCGGTCAG 
CCAGGAGACT GCTGTGATGT CTTTGAGTGC CAGAAAGTCT CTCCCAAGTG TGTCCACAAT 
GGGAAAGAGT TTTCCGAGGG CGAGGTGTAC CGCATGGACC CCTGCTGGCT GTGTCAGTGC 
CGCGGAGGAA TCTCCTTCTG CTCCAAGGCG GAGTGCGCTG AGCTGGACTG TGAAAACTTC 
TACATTCCTG AGGGCGAGTG TTGCCCTGTC TGCATAGATG TGGAGTTACT ATCAATGGAG 
AGCACTAAGG CCAGTTGCTG GGTGAATAAT AAGCTGCGGG CCCATGAGGA GCAGTGGAAA 
GAGGACGATT GCACCTTCTG CCAGTGTGTG GATGGAGAAC CGCACTGCAC GGCCATGGCC 
TGCAAACAGA GCTGCCAATA CCCTGTCAAG ATACCGGGAG AGTGCTGTCC CTTCTGTGAA 
GAGCCTTCTT ACGAAACTGT TAGCCCCATG CTATGCCCTC CTCTGGTAAA CTGCTCCCTG 
TCAGGGAATG ACTGCCTCTA CGGCTTCCAA CAAGACAGAA ATGGATGTCT GCTCTGCCAC 
TGTCTCAACA ATGACTCCTG CCCTGACCTG GGAAAATACT GTTCACTGCA ATGCCCAATG 
GGCTACAAGA AGGATGATTT TGGCTGTGAT GTGTGCGAGT GCAGCATGCC CAGGTGCCGA 
CCTTTGACCT GCACTAAGAC CTGCCCCTAT GGATATGTGA GAAACAAGCA TGGCTGTGAG 
ATGTGTCGCT GTGTCAAGTG TCCTCCCTTC ACCTGTGACA AGCACTGCAG CGACGGCTAT 
CGGCAGAACA GGAAGGGCTG CTCCATCTGC ATGTGTAAAG AAAGCAGCCA TGTCCCAAGC 
ACCACTCCCC TGGCCCCGAT GCCCAGCTAT TGCCTCACCT CCAATGGGCA CCGGTATGAG 
GAAGGCGACG GCTGGCACGA CGGTTGTCGT GACTGCTACT GCCACTCCGG ACGGGAGATG 
TGTGTGCTCA TTTCCTGCCC CGTACCCAGC TGTTCTCACC CAGTTGTAAG GCCTGACCAG 
TGTTGTCCTA CATGTGAGGA TGAGTCAGGC AGCGGTCTGC CAGAGGGGAT GGACATGGTG 
GTGTGCAGAG CACCTGGGGG AGAATTTTAT GTGGAGGGGG AGACCTGGAA CCTGGACGAG 
TGCACAAGGT GCACCTGCAG GAAAGGACGT GTCCTGTGCG ACACTGAAGT GTGTCCACCA 
GCTCTCTGCC AGACACCAAT CAGAAACAAG AACACCTGTT GCCACGTATG CCCAGAGGAG 
ACACTGAGCC CCTTGCTGCC AGTGAACAAC AGCCAGCAGG AGTACTGCAT AACCAGCGAT 
GGGGACGTGC TACTGGCCGG GGACTCCTGG AAGGCCAACG CCTGCACCAG CTGCACCTGC 
AACAATGGCA CCATTCAGTG TTTCTCTCAG CGCTGTCCGG CTGCCAACTG CAGGGTGCCC 
GTCTTACGCA AGGGCCAGTG CTGCCCACAC TGTCTCGAAA TTACAACGTC AGTACCGGTG 
ATGGTGTCTA CCGACGCCCC CAAGACAATG GTCCCTATTA CAGTGGAGAG TGCAGTGTGG 
ATGACCACAA TCACTGTACC ACCCATCATT GCTGAGACAG ATGAGCCAGG TTTGCGTGAG 
ATTTACCCCA GCCAAACAGA GATGGCCATC ATCTACCAAT CAGCAGCCTG GATCCTGGCA 
GGTCTCCTTC TGGCTATCAT TATCTTCCTC GTCGTGGCAT TAATAATCAA CAAGAAAAAG 
AAGTGGGTGC AGATGTCCTG CTACAGTGCA CCAAAGAAGA CGGTGATCCT GAAGAAGCAC 
GTAAACAAGA ACTCAGTGGT CTACATGGAG CCCTCCAAGG AGAACAAATT TCAGAATGTG 
AAGAAGGACT GCGGCATCAT CCATTTCTCT CCAGAAATGA GCTCCACCTG CGCAGACAAG 
ATGACCATCC CCAGGGCCAA GCTGAGCATG GGCAACGACT GGACAAAGCG CTAA