BRF2 cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRF2
Entrez Gene ID	102463193
Full Name	BRF2, RNA polymerase III transcription initiation factor subunit
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006134057.2	XP_006134119.1	transcription factor IIIB 50 kDa subunit
XM_006134057.3	XP_006134119.1	transcription factor IIIB 50 kDa subunit

Pathways from BioSystems
Gene Ontology

The following BRF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPe39149	XM_006134057.3 Latest version!	Pelodiscus sinensis BRF2, RNA polymerase III transcription initiation factor subunit (BRF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
View Old Accession Versions >>
OPe39149	XM_006134057.2	Pelodiscus sinensis BRF2, RNA polymerase III transcription initiation factor 50 kDa subunit (BRF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRF2 ( XM_006134057.2 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006134119.1 Pelodiscus sinensis BRF2, RNA polymerase III transcription initiation factor 50 kDa subunit (BRF2), mRNA.

Price & Availability↑

CloneID	OPe39149
Clone ID Related Accession (Same CDS sequence)	XM_006134057.2 , XM_006134057.3
Accession Version	XM_006134057.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	transcription factor IIIB 50 kDa subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005858300.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 3, 2015 this sequence version replaced XM_006134057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGTCGGGCC CGAGAAAATG CCCTGATTGT GGCTCCTCTG ACATCGTGGA CGATGCGCAT 
TATGCTCAGA ACCAACTGGT GTGCGCTGAC TGCGGGTTTG TTCTCACCGA GGGGCTCTTG 
ACAACCACTT TCCAAGATGA AGAGCATTTA CAAGAAGTGA CGTATTCACG GAGCACTGGG 
CAGAATGAGC AGCTGAGCCG CTGTACGCAA CGAGGGATCA AGCGTGTCCA GGATCTCTGC 
AAAGTCCTGC GTCTCCCGGC GGTGTTTGAA GACACAGCCC TGTCCTATTT CCAGCGAGCA 
ATTAAGCACC CCTCCTTCCA CTTGGTCCGC TTGGAGAAGA AGGAGATTCT GGTGGGCTGC 
TGTGTCTTTG TGACCTGCCG GCAGCACAAC TGGCCCCTGA CCATGGGCAC GATCTGCTCC 
CTGCTCTACG CAGACAAGGA GCTGTTTGCC AGCGTCTACC TAGCTGTCCT GAAAGAGCTG 
GAACTGGACG TGCCAGCCCT GAGTCTGATG GATCTGGTGA AAACGCACCT CAACAGTTTC 
AAGCTGTTCC AGAGTTCAGC CAGCATCCCT GCCAGATTTG CTGAGGACAA GGACAAGATG 
GTTGCCCGGA CAATCCAGAT TGTGGAGCTG GCCAGCGAGA CCTGGCTGGT GACCGGCCGG 
CATCCCATTC CCATCGTTCT TTCTCGACAA GGACAAGATG GTTGCCCGGA CAATCCAGAT 
TGTGGAGCTG GCCAGCGAGA CCTGGCTGGT GACCGGCCGG CATCCCATTC CCATCGTCCG 
GCCCACCTGA GGCTGAAGGA GCTGAACGAC ATCCTGCTGA GAATGGCCTC CCAGCTGGCC 
TGGCTGCGTG TGCTCCAGGT GGACAAAAAG ACTGGCTCTG AGAGTTCCCT GCGTGCGGCA 
GGGTGCGCTG GCCAAGCGGA GGGCTGCCCC CCGGGGGGCA AGCAGAAGAG CAGCACTGGC 
CAGAGGCCTT TGCTCCCACC GTGCCTTCTC AACCCCAGGA AGAGACTTCG CACAGCAGCC 
CCGAGTCCTG CGGAGCAGGC GATCACTGGG GATGAGGCCA TTTCAGACAG CGAAATAGAG 
CAGTACCTGC GGAGCCAGGA GGAGATGCGG GAGTTCCGCA AGGCCCAGGC CCAGGCCCGG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006134119.1
CDS	341..1486
Translation	MSGPRKCPDCGSSDIVDDAHYAQNQLVCADCGFVLTEGLLTTTFQDEEHLQEVTYSRSTGQNEQLSRCTQRGIKRVQDLCKVLRLPAVFEDTALSYFQRAIKHPSFHLVRLEKKEILVGCCVFVTCRQHNWPLTMGTICSLLYADKELFASVYLAVLKELELDVPALSLMDLVKTHLNSFKLFQSSASIPARFAEDKDKMVARTIQIVELASETWLVTGRHPIPIVLSRQGQDGCPDNPDCGAGQRDLAGDRPASHSHRPAHLRLKELNDILLRMASQLAWLRVLQVDKKTGSESSLRAAGCAGQAEGCPPGGKQKSSTGQRPLLPPCLLNPRKRLRTAAPSPAEQAITGDEAISDSEIEQYLRSQEEMREFRKAQAQARH

Target ORF information:

RefSeq Version	XM_006134057.2
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis BRF2, RNA polymerase III transcription initiation factor 50 kDa subunit (BRF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006134057.2

ORF Insert Sequence:

ATGTCGGGCC CGAGAAAATG CCCTGATTGT GGCTCCTCTG ACATCGTGGA CGATGCGCAT 
TATGCTCAGA ACCAACTGGT GTGCGCTGAC TGCGGGTTTG TTCTCACCGA GGGGCTCTTG 
ACAACCACTT TCCAAGATGA AGAGCATTTA CAAGAAGTGA CGTATTCACG GAGCACTGGG 
CAGAATGAGC AGCTGAGCCG CTGTACGCAA CGAGGGATCA AGCGTGTCCA GGATCTCTGC 
AAAGTCCTGC GTCTCCCGGC GGTGTTTGAA GACACAGCCC TGTCCTATTT CCAGCGAGCA 
ATTAAGCACC CCTCCTTCCA CTTGGTCCGC TTGGAGAAGA AGGAGATTCT GGTGGGCTGC 
TGTGTCTTTG TGACCTGCCG GCAGCACAAC TGGCCCCTGA CCATGGGCAC GATCTGCTCC 
CTGCTCTACG CAGACAAGGA GCTGTTTGCC AGCGTCTACC TAGCTGTCCT GAAAGAGCTG 
GAACTGGACG TGCCAGCCCT GAGTCTGATG GATCTGGTGA AAACGCACCT CAACAGTTTC 
AAGCTGTTCC AGAGTTCAGC CAGCATCCCT GCCAGATTTG CTGAGGACAA GGACAAGATG 
GTTGCCCGGA CAATCCAGAT TGTGGAGCTG GCCAGCGAGA CCTGGCTGGT GACCGGCCGG 
CATCCCATTC CCATCGTTCT TTCTCGACAA GGACAAGATG GTTGCCCGGA CAATCCAGAT 
TGTGGAGCTG GCCAGCGAGA CCTGGCTGGT GACCGGCCGG CATCCCATTC CCATCGTCCG 
GCCCACCTGA GGCTGAAGGA GCTGAACGAC ATCCTGCTGA GAATGGCCTC CCAGCTGGCC 
TGGCTGCGTG TGCTCCAGGT GGACAAAAAG ACTGGCTCTG AGAGTTCCCT GCGTGCGGCA 
GGGTGCGCTG GCCAAGCGGA GGGCTGCCCC CCGGGGGGCA AGCAGAAGAG CAGCACTGGC 
CAGAGGCCTT TGCTCCCACC GTGCCTTCTC AACCCCAGGA AGAGACTTCG CACAGCAGCC 
CCGAGTCCTG CGGAGCAGGC GATCACTGGG GATGAGGCCA TTTCAGACAG CGAAATAGAG 
CAGTACCTGC GGAGCCAGGA GGAGATGCGG GAGTTCCGCA AGGCCCAGGC CCAGGCCCGG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRF2 ( XM_006134057.3 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006134119.1 Pelodiscus sinensis BRF2, RNA polymerase III transcription initiation factor subunit (BRF2), mRNA.

Price & Availability↑

CloneID	OPe39149
Clone ID Related Accession (Same CDS sequence)	XM_006134057.2 , XM_006134057.3
Accession Version	XM_006134057.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	transcription factor IIIB 50 kDa subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005858300.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 4, 2018 this sequence version replaced XM_006134057.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCGGGCC CGAGAAAATG CCCTGATTGT GGCTCCTCTG ACATCGTGGA CGATGCGCAT 
TATGCTCAGA ACCAACTGGT GTGCGCTGAC TGCGGGTTTG TTCTCACCGA GGGGCTCTTG 
ACAACCACTT TCCAAGATGA AGAGCATTTA CAAGAAGTGA CGTATTCACG GAGCACTGGG 
CAGAATGAGC AGCTGAGCCG CTGTACGCAA CGAGGGATCA AGCGTGTCCA GGATCTCTGC 
AAAGTCCTGC GTCTCCCGGC GGTGTTTGAA GACACAGCCC TGTCCTATTT CCAGCGAGCA 
ATTAAGCACC CCTCCTTCCA CTTGGTCCGC TTGGAGAAGA AGGAGATTCT GGTGGGCTGC 
TGTGTCTTTG TGACCTGCCG GCAGCACAAC TGGCCCCTGA CCATGGGCAC GATCTGCTCC 
CTGCTCTACG CAGACAAGGA GCTGTTTGCC AGCGTCTACC TAGCTGTCCT GAAAGAGCTG 
GAACTGGACG TGCCAGCCCT GAGTCTGATG GATCTGGTGA AAACGCACCT CAACAGTTTC 
AAGCTGTTCC AGAGTTCAGC CAGCATCCCT GCCAGATTTG CTGAGGACAA GGACAAGATG 
GTTGCCCGGA CAATCCAGAT TGTGGAGCTG GCCAGCGAGA CCTGGCTGGT GACCGGCCGG 
CATCCCATTC CCATCGTTCT TTCTCGACAA GGACAAGATG GTTGCCCGGA CAATCCAGAT 
TGTGGAGCTG GCCAGCGAGA CCTGGCTGGT GACCGGCCGG CATCCCATTC CCATCGTCCG 
GCCCACCTGA GGCTGAAGGA GCTGAACGAC ATCCTGCTGA GAATGGCCTC CCAGCTGGCC 
TGGCTGCGTG TGCTCCAGGT GGACAAAAAG ACTGGCTCTG AGAGTTCCCT GCGTGCGGCA 
GGGTGCGCTG GCCAAGCGGA GGGCTGCCCC CCGGGGGGCA AGCAGAAGAG CAGCACTGGC 
CAGAGGCCTT TGCTCCCACC GTGCCTTCTC AACCCCAGGA AGAGACTTCG CACAGCAGCC 
CCGAGTCCTG CGGAGCAGGC GATCACTGGG GATGAGGCCA TTTCAGACAG CGAAATAGAG 
CAGTACCTGC GGAGCCAGGA GGAGATGCGG GAGTTCCGCA AGGCCCAGGC CCAGGCCCGG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006134119.1
CDS	368..1513
Translation	MSGPRKCPDCGSSDIVDDAHYAQNQLVCADCGFVLTEGLLTTTFQDEEHLQEVTYSRSTGQNEQLSRCTQRGIKRVQDLCKVLRLPAVFEDTALSYFQRAIKHPSFHLVRLEKKEILVGCCVFVTCRQHNWPLTMGTICSLLYADKELFASVYLAVLKELELDVPALSLMDLVKTHLNSFKLFQSSASIPARFAEDKDKMVARTIQIVELASETWLVTGRHPIPIVLSRQGQDGCPDNPDCGAGQRDLAGDRPASHSHRPAHLRLKELNDILLRMASQLAWLRVLQVDKKTGSESSLRAAGCAGQAEGCPPGGKQKSSTGQRPLLPPCLLNPRKRLRTAAPSPAEQAITGDEAISDSEIEQYLRSQEEMREFRKAQAQARH

Target ORF information:

RefSeq Version	XM_006134057.3
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis BRF2, RNA polymerase III transcription initiation factor subunit (BRF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006134057.3

ORF Insert Sequence:

ATGTCGGGCC CGAGAAAATG CCCTGATTGT GGCTCCTCTG ACATCGTGGA CGATGCGCAT 
TATGCTCAGA ACCAACTGGT GTGCGCTGAC TGCGGGTTTG TTCTCACCGA GGGGCTCTTG 
ACAACCACTT TCCAAGATGA AGAGCATTTA CAAGAAGTGA CGTATTCACG GAGCACTGGG 
CAGAATGAGC AGCTGAGCCG CTGTACGCAA CGAGGGATCA AGCGTGTCCA GGATCTCTGC 
AAAGTCCTGC GTCTCCCGGC GGTGTTTGAA GACACAGCCC TGTCCTATTT CCAGCGAGCA 
ATTAAGCACC CCTCCTTCCA CTTGGTCCGC TTGGAGAAGA AGGAGATTCT GGTGGGCTGC 
TGTGTCTTTG TGACCTGCCG GCAGCACAAC TGGCCCCTGA CCATGGGCAC GATCTGCTCC 
CTGCTCTACG CAGACAAGGA GCTGTTTGCC AGCGTCTACC TAGCTGTCCT GAAAGAGCTG 
GAACTGGACG TGCCAGCCCT GAGTCTGATG GATCTGGTGA AAACGCACCT CAACAGTTTC 
AAGCTGTTCC AGAGTTCAGC CAGCATCCCT GCCAGATTTG CTGAGGACAA GGACAAGATG 
GTTGCCCGGA CAATCCAGAT TGTGGAGCTG GCCAGCGAGA CCTGGCTGGT GACCGGCCGG 
CATCCCATTC CCATCGTTCT TTCTCGACAA GGACAAGATG GTTGCCCGGA CAATCCAGAT 
TGTGGAGCTG GCCAGCGAGA CCTGGCTGGT GACCGGCCGG CATCCCATTC CCATCGTCCG 
GCCCACCTGA GGCTGAAGGA GCTGAACGAC ATCCTGCTGA GAATGGCCTC CCAGCTGGCC 
TGGCTGCGTG TGCTCCAGGT GGACAAAAAG ACTGGCTCTG AGAGTTCCCT GCGTGCGGCA 
GGGTGCGCTG GCCAAGCGGA GGGCTGCCCC CCGGGGGGCA AGCAGAAGAG CAGCACTGGC 
CAGAGGCCTT TGCTCCCACC GTGCCTTCTC AACCCCAGGA AGAGACTTCG CACAGCAGCC 
CCGAGTCCTG CGGAGCAGGC GATCACTGGG GATGAGGCCA TTTCAGACAG CGAAATAGAG 
CAGTACCTGC GGAGCCAGGA GGAGATGCGG GAGTTCCGCA AGGCCCAGGC CCAGGCCCGG 
CACTGA