DSEL cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DSEL
Entrez Gene ID	102455303
Full Name	dermatan sulfate epimerase-like
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006118124.2	XP_006118186.1	dermatan-sulfate epimerase-like protein
XM_006118124.3	XP_006118186.1	dermatan-sulfate epimerase-like protein

Pathways from BioSystems
Gene Ontology

The following DSEL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DSEL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPe24820	XM_006118124.3 Latest version!	Pelodiscus sinensis dermatan sulfate epimerase like (DSEL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
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OPe24820	XM_006118124.2	Pelodiscus sinensis dermatan sulfate epimerase-like (DSEL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DSEL ( XM_006118124.2 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006118186.1 Pelodiscus sinensis dermatan sulfate epimerase-like (DSEL), mRNA.

Price & Availability↑

CloneID	OPe24820
Clone ID Related Accession (Same CDS sequence)	XM_006118124.2 , XM_006118124.3
Accession Version	XM_006118124.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	dermatan-sulfate epimerase-like protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005853736.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 3, 2015 this sequence version replaced XM_006118124.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTA TGTTTACACT CCTAGTATTA GCAATGTTTG CGCTCTCCAC TTTTGAAGAA 
TCTGTAAGCA ATTATTCTGA CTGGGTGACT TTTGCAGAAA ATGTGGCTCA ATATGAGAAA 
CAGAAATTAC AAGATTTCAG TGCTGATCAG AAGCTGAAAA AAGTCGTTCT TCATCCAAGC 
TTATATTTTG ATGCTGAAGA TGTCCAAACA CTGAGGCAGA AGTCTCGTAC AAGCCATTTA 
CATCTCTTCA GAGCTATCAG AGGTGCAGTG ACAGTTATGC TGTCCAACCC ATCATACTAC 
CTACCTCCAC CCAAGCATGC TGATTTTGCT GCAAAGTGGA ATGAGATTTA TGGTAACAAT 
CTGCCTCCCT TAGCTTTTTA TTGTTTGCTG TGCCCGGAAG ATAAAGCTGC CTTTGATTTT 
GTTCTAGAGT ACATGGACAG AATGAGTGGC TACAAAGACT GGCTGGTTGA GAATGCTCCA 
GGAGATGAAG TGCCACTTGG CCACTCCTTA ACAGGTTTTG CCACTGCTTT TGACTTCTTA 
TATAGTTCAC TGGATAATAT CAGAAGACAA AAATATCTAA AGAAGATATG GGCTGTGAGT 
GAGGAAATGT ATGAATATTC AAAAGTCCGC TCATGGGGAA AGCAACTTCT CCATAACCAC 
CAAGCCACCA ATATGCTAGC ACTGCTCACG GGGGCCCTGG TTACTGGTGT GGACAAAGAA 
TCTCAGACAA ATGTCTGGAA GCATGCTGTC GTCGATATGA TGGAAAAAAC CATGTTTCTC 
CTCAACCACA TTGTAGATGG CTCTTTGGAT GAAGGAGTCG CATATGGCAG TTACACAGCT 
AAGTCAGTAA CCCAATATGT TTTTCTGGCC CAGCGCCATT TTGGTATTAA TAACCTAGAA 
AACAATTGGT TGAAAATGCA CTTTTGGTTT TATTATGCCA CACTTTTACC CGGCTATCAG 
AGGACTGTGG GCATAGCAGA TTCTAATTAT AACTGGTTTT ATGGCCCTGA AAGCCAGCTG 
GTTTTCCTGG ATAAGTTTGT CCTGAAGAAT GGAGCTGGCA ACTGGTTGGC ACAACAGATT 
AGAAAGCACA GGCCCAAAGA TGGCCCAATG GTACCATCCA CTGCACAAAG GTGGAGCACG 
CTTCACACGG AGTACATATG GTATGCTGCA GAACTAACTC CTCAGCCTCC CCTGGACTAT 
GGCACTGCTC AGATGCATGT GTTTCCTAAC TGGGGGGTTG TTACTTATGG GGCTGGGTTG 
CCGAATACTC AGACCAATAC CTTTCTGTCT TTTAAATCTG GAAAGCTTGG CGGGCGTGCA 
GTCTATGACA TTGTTCACTT TCAGCCATAT TCCTGGATTG ATGGGTGGAG GAGTTTCAAT 
CCAGGGCATG AACATCCAGA TCAGAACTCC TTTACTTTTG CTCCCAATGG ACAGGTGTTT 
GTATCTGAGG CCCTATATGG ACCCAAGTTC AGCCACTTGA ATAACGTACT GGTGTTTGCT 
CCATCTCCTA CAAGTCAGTG TAATCAGCCT TGGGAGGGAC AGCTTGGGGA GTGCGCACAG 
TGGCTTAAGT GGACTGGTGA TGAGGTTGGA GACTCAGCTG GGGAGATAAT AACAGCATCT 
CAATATGGGG AGATGATGTT TGTAAGCGGT GAGGCAGCTT CTGCCTATTC TTCTGCAATG 
AAATTAAAAA GTGTGTATCG TACTTTGCTA CTCCTAAATG CTCAGACACT GCTAGTAGTA 
GACCATATTG AGAAGGAGAA AGACTCTCCC CTCAGCTCTG TCAGTGCCTT CTTTCATAAC 
CTTGACATTG ATTTTAAATA TATACCCTAT ATGTTTATGA ACAAATATAA CGGAGCCATG 
ATGGATGTGT GGGATGCTCA CTATAAGATG TTTTGGTTTG ATCATCACGG GAGTAGTCCT 
GTTGCCAGGA TACAAGAGGC AGAGCAAGCA GCAGAGTTCA AAAAGCGGTG GACTCAGTTC 
ATAAACGTTA CCTTTCCTAT GAAAACCACT GTAACAAGAA TTGCGTATGT GTTCCATGGA 
CCATATGTTA ATGTTTCTAA CTGCAGGTTC ATAGATGATT CCAAATACGG ATTTCAGATT 
TTTCTAAATG TCAACAACAC TGAAAATATT GTGTCTGTTG TGACTGAATA TCACAGTCTG 
AGGACAAGGT TCAATTATTT GGGATTTGGT GGCTTTGCAA AGGTAGCTGA CCAAAACAAA 
GTTATCAAAT TTGGTCTAGG CACTGAAACT GTACAAAGAC AGACAACAAA TAATAGTATC 
GTTTTCCCAT TTGGATTTAA AGTGAATATA ATAGTAGGAT TAGTTTTGAG CATTAGTTTG 
GTAATACTGG CTTTTCAGTG GCGGTTTTAC ATTTCCTTCA GCAAATTGTT GCGCTGGATA 
CTAATATTGG TTATCACACT GTGGTTTATT GAATTGATGG ATGTTTGGAG CACTTGCACT 
CAGCCTATCT GTGCAAAATG GAACAGTGAC ATCATAAACG TAGACCCTGA TGCAGGCAAT 
AAAGCTAAAC AGTCAGAAGG AAACCCTGTT GTTTTGCCAG ATGTTGTGAT TACCTCACTT 
CCCAGTTCTG GAGCAGAAAT TTTAAAGCAG CTTTTTTTTA ACAGCAGTGA TTTCATATAC 
ATCAGGATCC CTACAACTTA CCTTGATATC CCTGAAACTG AATTTGAAAT AGACTCATTT 
GTAGATGCGT GTGAATGGAA GGCGTCTGAT GTCCAGAGTG GCCATTTCCA TGTACTTCGA 
GGCTGGCTCC AGTCTCTAGT CCAAGACACA AAACTTCATT TACAAAATAT TCATTTACAT 
GAAGGCAGCA GAAGCAAAGT TGCTCAGCAT TCGATTGTAA ATAAGGACAA AAAAAGAAGA 
GCCAGAAAGA GAGAATCTTT GTCCGAACAA AGAAGCAGAG CAAGAGGAAA ATTAGACAAA 
GATGCTGAAT ATGTTAGGGA ACTGAGGAGA CACCTTGCCT ATTATCCAAA TGCACGTCCT 
GTGCTTAGTT TAAGTAGTGG GAGCTGGACA TTAAAGCTTC CCTTTTTTCA AGAAATCATA 
GGGCCTTCAA TGAGGGCATT GTATGTAGTA AGGGACCCTA GGGCATGGAT CTACTCAATG 
TTGCATAAGA GTAAGCCAAG CCTTTATTCC TTGAAAAACG TTCCACAGCA CTTAGCAATG 
ATGTTTAAAG TGGAAGACGG GAAAGAAAAA TGCAGTTTAA ATTCAGGCTA TGCCTTTGAA 
TATGAAGCAC TAAGAGAAGA GCTCATGGAT TCCAGGTCAA ATGCAGTTTC TGTGCTGTCC 
CATTTGTGGC TAGCAAACAC AGCAGCAGCA CTGCGAGTAA ATGGGTATCT ACTGCAGACA 
AATTATCAGC TGGTCAAGTT TGAAGATATC GTGAGCTCTC CCCAGAAGAC AGCGGAAAGA 
ATTTATGCTT TTCTTGGTAT TCCTCTGTCT CCTGCTAGCT TAAACCAAAT ATTGTTTGCC 
ACCTCCACCA ATCTTTTCTA TCTTCCTTAT GAGGGTGAAA TATCACCCAT TAGTATTCAA 
GCCTGGGAAC AAGACATGCC TGTTGAAGAT ATTAGACTGA TTGAGGACAT CTGCTGTACT 
GTGATGGGCC ACTTAGGATA CCCAAAGTTT ACAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006118186.1
CDS	1840..5478
Translation	MALMFTLLVLAMFALSTFEESVSNYSDWVTFAENVAQYEKQKLQDFSADQKLKKVVLHPSLYFDAEDVQTLRQKSRTSHLHLFRAIRGAVTVMLSNPSYYLPPPKHADFAAKWNEIYGNNLPPLAFYCLLCPEDKAAFDFVLEYMDRMSGYKDWLVENAPGDEVPLGHSLTGFATAFDFLYSSLDNIRRQKYLKKIWAVSEEMYEYSKVRSWGKQLLHNHQATNMLALLTGALVTGVDKESQTNVWKHAVVDMMEKTMFLLNHIVDGSLDEGVAYGSYTAKSVTQYVFLAQRHFGINNLENNWLKMHFWFYYATLLPGYQRTVGIADSNYNWFYGPESQLVFLDKFVLKNGAGNWLAQQIRKHRPKDGPMVPSTAQRWSTLHTEYIWYAAELTPQPPLDYGTAQMHVFPNWGVVTYGAGLPNTQTNTFLSFKSGKLGGRAVYDIVHFQPYSWIDGWRSFNPGHEHPDQNSFTFAPNGQVFVSEALYGPKFSHLNNVLVFAPSPTSQCNQPWEGQLGECAQWLKWTGDEVGDSAGEIITASQYGEMMFVSGEAASAYSSAMKLKSVYRTLLLLNAQTLLVVDHIEKEKDSPLSSVSAFFHNLDIDFKYIPYMFMNKYNGAMMDVWDAHYKMFWFDHHGSSPVARIQEAEQAAEFKKRWTQFINVTFPMKTTVTRIAYVFHGPYVNVSNCRFIDDSKYGFQIFLNVNNTENIVSVVTEYHSLRTRFNYLGFGGFAKVADQNKVIKFGLGTETVQRQTTNNSIVFPFGFKVNIIVGLVLSISLVILAFQWRFYISFSKLLRWILILVITLWFIELMDVWSTCTQPICAKWNSDIINVDPDAGNKAKQSEGNPVVLPDVVITSLPSSGAEILKQLFFNSSDFIYIRIPTTYLDIPETEFEIDSFVDACEWKASDVQSGHFHVLRGWLQSLVQDTKLHLQNIHLHEGSRSKVAQHSIVNKDKKRRARKRESLSEQRSRARGKLDKDAEYVRELRRHLAYYPNARPVLSLSSGSWTLKLPFFQEIIGPSMRALYVVRDPRAWIYSMLHKSKPSLYSLKNVPQHLAMMFKVEDGKEKCSLNSGYAFEYEALREELMDSRSNAVSVLSHLWLANTAAALRVNGYLLQTNYQLVKFEDIVSSPQKTAERIYAFLGIPLSPASLNQILFATSTNLFYLPYEGEISPISIQAWEQDMPVEDIRLIEDICCTVMGHLGYPKFTV

Target ORF information:

RefSeq Version	XM_006118124.2
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis dermatan sulfate epimerase-like (DSEL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006118124.2

ORF Insert Sequence:

ATGGCTCTTA TGTTTACACT CCTAGTATTA GCAATGTTTG CGCTCTCCAC TTTTGAAGAA 
TCTGTAAGCA ATTATTCTGA CTGGGTGACT TTTGCAGAAA ATGTGGCTCA ATATGAGAAA 
CAGAAATTAC AAGATTTCAG TGCTGATCAG AAGCTGAAAA AAGTCGTTCT TCATCCAAGC 
TTATATTTTG ATGCTGAAGA TGTCCAAACA CTGAGGCAGA AGTCTCGTAC AAGCCATTTA 
CATCTCTTCA GAGCTATCAG AGGTGCAGTG ACAGTTATGC TGTCCAACCC ATCATACTAC 
CTACCTCCAC CCAAGCATGC TGATTTTGCT GCAAAGTGGA ATGAGATTTA TGGTAACAAT 
CTGCCTCCCT TAGCTTTTTA TTGTTTGCTG TGCCCGGAAG ATAAAGCTGC CTTTGATTTT 
GTTCTAGAGT ACATGGACAG AATGAGTGGC TACAAAGACT GGCTGGTTGA GAATGCTCCA 
GGAGATGAAG TGCCACTTGG CCACTCCTTA ACAGGTTTTG CCACTGCTTT TGACTTCTTA 
TATAGTTCAC TGGATAATAT CAGAAGACAA AAATATCTAA AGAAGATATG GGCTGTGAGT 
GAGGAAATGT ATGAATATTC AAAAGTCCGC TCATGGGGAA AGCAACTTCT CCATAACCAC 
CAAGCCACCA ATATGCTAGC ACTGCTCACG GGGGCCCTGG TTACTGGTGT GGACAAAGAA 
TCTCAGACAA ATGTCTGGAA GCATGCTGTC GTCGATATGA TGGAAAAAAC CATGTTTCTC 
CTCAACCACA TTGTAGATGG CTCTTTGGAT GAAGGAGTCG CATATGGCAG TTACACAGCT 
AAGTCAGTAA CCCAATATGT TTTTCTGGCC CAGCGCCATT TTGGTATTAA TAACCTAGAA 
AACAATTGGT TGAAAATGCA CTTTTGGTTT TATTATGCCA CACTTTTACC CGGCTATCAG 
AGGACTGTGG GCATAGCAGA TTCTAATTAT AACTGGTTTT ATGGCCCTGA AAGCCAGCTG 
GTTTTCCTGG ATAAGTTTGT CCTGAAGAAT GGAGCTGGCA ACTGGTTGGC ACAACAGATT 
AGAAAGCACA GGCCCAAAGA TGGCCCAATG GTACCATCCA CTGCACAAAG GTGGAGCACG 
CTTCACACGG AGTACATATG GTATGCTGCA GAACTAACTC CTCAGCCTCC CCTGGACTAT 
GGCACTGCTC AGATGCATGT GTTTCCTAAC TGGGGGGTTG TTACTTATGG GGCTGGGTTG 
CCGAATACTC AGACCAATAC CTTTCTGTCT TTTAAATCTG GAAAGCTTGG CGGGCGTGCA 
GTCTATGACA TTGTTCACTT TCAGCCATAT TCCTGGATTG ATGGGTGGAG GAGTTTCAAT 
CCAGGGCATG AACATCCAGA TCAGAACTCC TTTACTTTTG CTCCCAATGG ACAGGTGTTT 
GTATCTGAGG CCCTATATGG ACCCAAGTTC AGCCACTTGA ATAACGTACT GGTGTTTGCT 
CCATCTCCTA CAAGTCAGTG TAATCAGCCT TGGGAGGGAC AGCTTGGGGA GTGCGCACAG 
TGGCTTAAGT GGACTGGTGA TGAGGTTGGA GACTCAGCTG GGGAGATAAT AACAGCATCT 
CAATATGGGG AGATGATGTT TGTAAGCGGT GAGGCAGCTT CTGCCTATTC TTCTGCAATG 
AAATTAAAAA GTGTGTATCG TACTTTGCTA CTCCTAAATG CTCAGACACT GCTAGTAGTA 
GACCATATTG AGAAGGAGAA AGACTCTCCC CTCAGCTCTG TCAGTGCCTT CTTTCATAAC 
CTTGACATTG ATTTTAAATA TATACCCTAT ATGTTTATGA ACAAATATAA CGGAGCCATG 
ATGGATGTGT GGGATGCTCA CTATAAGATG TTTTGGTTTG ATCATCACGG GAGTAGTCCT 
GTTGCCAGGA TACAAGAGGC AGAGCAAGCA GCAGAGTTCA AAAAGCGGTG GACTCAGTTC 
ATAAACGTTA CCTTTCCTAT GAAAACCACT GTAACAAGAA TTGCGTATGT GTTCCATGGA 
CCATATGTTA ATGTTTCTAA CTGCAGGTTC ATAGATGATT CCAAATACGG ATTTCAGATT 
TTTCTAAATG TCAACAACAC TGAAAATATT GTGTCTGTTG TGACTGAATA TCACAGTCTG 
AGGACAAGGT TCAATTATTT GGGATTTGGT GGCTTTGCAA AGGTAGCTGA CCAAAACAAA 
GTTATCAAAT TTGGTCTAGG CACTGAAACT GTACAAAGAC AGACAACAAA TAATAGTATC 
GTTTTCCCAT TTGGATTTAA AGTGAATATA ATAGTAGGAT TAGTTTTGAG CATTAGTTTG 
GTAATACTGG CTTTTCAGTG GCGGTTTTAC ATTTCCTTCA GCAAATTGTT GCGCTGGATA 
CTAATATTGG TTATCACACT GTGGTTTATT GAATTGATGG ATGTTTGGAG CACTTGCACT 
CAGCCTATCT GTGCAAAATG GAACAGTGAC ATCATAAACG TAGACCCTGA TGCAGGCAAT 
AAAGCTAAAC AGTCAGAAGG AAACCCTGTT GTTTTGCCAG ATGTTGTGAT TACCTCACTT 
CCCAGTTCTG GAGCAGAAAT TTTAAAGCAG CTTTTTTTTA ACAGCAGTGA TTTCATATAC 
ATCAGGATCC CTACAACTTA CCTTGATATC CCTGAAACTG AATTTGAAAT AGACTCATTT 
GTAGATGCGT GTGAATGGAA GGCGTCTGAT GTCCAGAGTG GCCATTTCCA TGTACTTCGA 
GGCTGGCTCC AGTCTCTAGT CCAAGACACA AAACTTCATT TACAAAATAT TCATTTACAT 
GAAGGCAGCA GAAGCAAAGT TGCTCAGCAT TCGATTGTAA ATAAGGACAA AAAAAGAAGA 
GCCAGAAAGA GAGAATCTTT GTCCGAACAA AGAAGCAGAG CAAGAGGAAA ATTAGACAAA 
GATGCTGAAT ATGTTAGGGA ACTGAGGAGA CACCTTGCCT ATTATCCAAA TGCACGTCCT 
GTGCTTAGTT TAAGTAGTGG GAGCTGGACA TTAAAGCTTC CCTTTTTTCA AGAAATCATA 
GGGCCTTCAA TGAGGGCATT GTATGTAGTA AGGGACCCTA GGGCATGGAT CTACTCAATG 
TTGCATAAGA GTAAGCCAAG CCTTTATTCC TTGAAAAACG TTCCACAGCA CTTAGCAATG 
ATGTTTAAAG TGGAAGACGG GAAAGAAAAA TGCAGTTTAA ATTCAGGCTA TGCCTTTGAA 
TATGAAGCAC TAAGAGAAGA GCTCATGGAT TCCAGGTCAA ATGCAGTTTC TGTGCTGTCC 
CATTTGTGGC TAGCAAACAC AGCAGCAGCA CTGCGAGTAA ATGGGTATCT ACTGCAGACA 
AATTATCAGC TGGTCAAGTT TGAAGATATC GTGAGCTCTC CCCAGAAGAC AGCGGAAAGA 
ATTTATGCTT TTCTTGGTAT TCCTCTGTCT CCTGCTAGCT TAAACCAAAT ATTGTTTGCC 
ACCTCCACCA ATCTTTTCTA TCTTCCTTAT GAGGGTGAAA TATCACCCAT TAGTATTCAA 
GCCTGGGAAC AAGACATGCC TGTTGAAGAT ATTAGACTGA TTGAGGACAT CTGCTGTACT 
GTGATGGGCC ACTTAGGATA CCCAAAGTTT ACAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DSEL ( XM_006118124.3 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006118186.1 Pelodiscus sinensis dermatan sulfate epimerase like (DSEL), mRNA.

Price & Availability↑

CloneID	OPe24820
Clone ID Related Accession (Same CDS sequence)	XM_006118124.2 , XM_006118124.3
Accession Version	XM_006118124.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	dermatan-sulfate epimerase-like protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005853736.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 4, 2018 this sequence version replaced XM_006118124.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTA TGTTTACACT CCTAGTATTA GCAATGTTTG CGCTCTCCAC TTTTGAAGAA 
TCTGTAAGCA ATTATTCTGA CTGGGTGACT TTTGCAGAAA ATGTGGCTCA ATATGAGAAA 
CAGAAATTAC AAGATTTCAG TGCTGATCAG AAGCTGAAAA AAGTCGTTCT TCATCCAAGC 
TTATATTTTG ATGCTGAAGA TGTCCAAACA CTGAGGCAGA AGTCTCGTAC AAGCCATTTA 
CATCTCTTCA GAGCTATCAG AGGTGCAGTG ACAGTTATGC TGTCCAACCC ATCATACTAC 
CTACCTCCAC CCAAGCATGC TGATTTTGCT GCAAAGTGGA ATGAGATTTA TGGTAACAAT 
CTGCCTCCCT TAGCTTTTTA TTGTTTGCTG TGCCCGGAAG ATAAAGCTGC CTTTGATTTT 
GTTCTAGAGT ACATGGACAG AATGAGTGGC TACAAAGACT GGCTGGTTGA GAATGCTCCA 
GGAGATGAAG TGCCACTTGG CCACTCCTTA ACAGGTTTTG CCACTGCTTT TGACTTCTTA 
TATAGTTCAC TGGATAATAT CAGAAGACAA AAATATCTAA AGAAGATATG GGCTGTGAGT 
GAGGAAATGT ATGAATATTC AAAAGTCCGC TCATGGGGAA AGCAACTTCT CCATAACCAC 
CAAGCCACCA ATATGCTAGC ACTGCTCACG GGGGCCCTGG TTACTGGTGT GGACAAAGAA 
TCTCAGACAA ATGTCTGGAA GCATGCTGTC GTCGATATGA TGGAAAAAAC CATGTTTCTC 
CTCAACCACA TTGTAGATGG CTCTTTGGAT GAAGGAGTCG CATATGGCAG TTACACAGCT 
AAGTCAGTAA CCCAATATGT TTTTCTGGCC CAGCGCCATT TTGGTATTAA TAACCTAGAA 
AACAATTGGT TGAAAATGCA CTTTTGGTTT TATTATGCCA CACTTTTACC CGGCTATCAG 
AGGACTGTGG GCATAGCAGA TTCTAATTAT AACTGGTTTT ATGGCCCTGA AAGCCAGCTG 
GTTTTCCTGG ATAAGTTTGT CCTGAAGAAT GGAGCTGGCA ACTGGTTGGC ACAACAGATT 
AGAAAGCACA GGCCCAAAGA TGGCCCAATG GTACCATCCA CTGCACAAAG GTGGAGCACG 
CTTCACACGG AGTACATATG GTATGCTGCA GAACTAACTC CTCAGCCTCC CCTGGACTAT 
GGCACTGCTC AGATGCATGT GTTTCCTAAC TGGGGGGTTG TTACTTATGG GGCTGGGTTG 
CCGAATACTC AGACCAATAC CTTTCTGTCT TTTAAATCTG GAAAGCTTGG CGGGCGTGCA 
GTCTATGACA TTGTTCACTT TCAGCCATAT TCCTGGATTG ATGGGTGGAG GAGTTTCAAT 
CCAGGGCATG AACATCCAGA TCAGAACTCC TTTACTTTTG CTCCCAATGG ACAGGTGTTT 
GTATCTGAGG CCCTATATGG ACCCAAGTTC AGCCACTTGA ATAACGTACT GGTGTTTGCT 
CCATCTCCTA CAAGTCAGTG TAATCAGCCT TGGGAGGGAC AGCTTGGGGA GTGCGCACAG 
TGGCTTAAGT GGACTGGTGA TGAGGTTGGA GACTCAGCTG GGGAGATAAT AACAGCATCT 
CAATATGGGG AGATGATGTT TGTAAGCGGT GAGGCAGCTT CTGCCTATTC TTCTGCAATG 
AAATTAAAAA GTGTGTATCG TACTTTGCTA CTCCTAAATG CTCAGACACT GCTAGTAGTA 
GACCATATTG AGAAGGAGAA AGACTCTCCC CTCAGCTCTG TCAGTGCCTT CTTTCATAAC 
CTTGACATTG ATTTTAAATA TATACCCTAT ATGTTTATGA ACAAATATAA CGGAGCCATG 
ATGGATGTGT GGGATGCTCA CTATAAGATG TTTTGGTTTG ATCATCACGG GAGTAGTCCT 
GTTGCCAGGA TACAAGAGGC AGAGCAAGCA GCAGAGTTCA AAAAGCGGTG GACTCAGTTC 
ATAAACGTTA CCTTTCCTAT GAAAACCACT GTAACAAGAA TTGCGTATGT GTTCCATGGA 
CCATATGTTA ATGTTTCTAA CTGCAGGTTC ATAGATGATT CCAAATACGG ATTTCAGATT 
TTTCTAAATG TCAACAACAC TGAAAATATT GTGTCTGTTG TGACTGAATA TCACAGTCTG 
AGGACAAGGT TCAATTATTT GGGATTTGGT GGCTTTGCAA AGGTAGCTGA CCAAAACAAA 
GTTATCAAAT TTGGTCTAGG CACTGAAACT GTACAAAGAC AGACAACAAA TAATAGTATC 
GTTTTCCCAT TTGGATTTAA AGTGAATATA ATAGTAGGAT TAGTTTTGAG CATTAGTTTG 
GTAATACTGG CTTTTCAGTG GCGGTTTTAC ATTTCCTTCA GCAAATTGTT GCGCTGGATA 
CTAATATTGG TTATCACACT GTGGTTTATT GAATTGATGG ATGTTTGGAG CACTTGCACT 
CAGCCTATCT GTGCAAAATG GAACAGTGAC ATCATAAACG TAGACCCTGA TGCAGGCAAT 
AAAGCTAAAC AGTCAGAAGG AAACCCTGTT GTTTTGCCAG ATGTTGTGAT TACCTCACTT 
CCCAGTTCTG GAGCAGAAAT TTTAAAGCAG CTTTTTTTTA ACAGCAGTGA TTTCATATAC 
ATCAGGATCC CTACAACTTA CCTTGATATC CCTGAAACTG AATTTGAAAT AGACTCATTT 
GTAGATGCGT GTGAATGGAA GGCGTCTGAT GTCCAGAGTG GCCATTTCCA TGTACTTCGA 
GGCTGGCTCC AGTCTCTAGT CCAAGACACA AAACTTCATT TACAAAATAT TCATTTACAT 
GAAGGCAGCA GAAGCAAAGT TGCTCAGCAT TCGATTGTAA ATAAGGACAA AAAAAGAAGA 
GCCAGAAAGA GAGAATCTTT GTCCGAACAA AGAAGCAGAG CAAGAGGAAA ATTAGACAAA 
GATGCTGAAT ATGTTAGGGA ACTGAGGAGA CACCTTGCCT ATTATCCAAA TGCACGTCCT 
GTGCTTAGTT TAAGTAGTGG GAGCTGGACA TTAAAGCTTC CCTTTTTTCA AGAAATCATA 
GGGCCTTCAA TGAGGGCATT GTATGTAGTA AGGGACCCTA GGGCATGGAT CTACTCAATG 
TTGCATAAGA GTAAGCCAAG CCTTTATTCC TTGAAAAACG TTCCACAGCA CTTAGCAATG 
ATGTTTAAAG TGGAAGACGG GAAAGAAAAA TGCAGTTTAA ATTCAGGCTA TGCCTTTGAA 
TATGAAGCAC TAAGAGAAGA GCTCATGGAT TCCAGGTCAA ATGCAGTTTC TGTGCTGTCC 
CATTTGTGGC TAGCAAACAC AGCAGCAGCA CTGCGAGTAA ATGGGTATCT ACTGCAGACA 
AATTATCAGC TGGTCAAGTT TGAAGATATC GTGAGCTCTC CCCAGAAGAC AGCGGAAAGA 
ATTTATGCTT TTCTTGGTAT TCCTCTGTCT CCTGCTAGCT TAAACCAAAT ATTGTTTGCC 
ACCTCCACCA ATCTTTTCTA TCTTCCTTAT GAGGGTGAAA TATCACCCAT TAGTATTCAA 
GCCTGGGAAC AAGACATGCC TGTTGAAGAT ATTAGACTGA TTGAGGACAT CTGCTGTACT 
GTGATGGGCC ACTTAGGATA CCCAAAGTTT ACAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006118186.1
CDS	1862..5500
Translation	MALMFTLLVLAMFALSTFEESVSNYSDWVTFAENVAQYEKQKLQDFSADQKLKKVVLHPSLYFDAEDVQTLRQKSRTSHLHLFRAIRGAVTVMLSNPSYYLPPPKHADFAAKWNEIYGNNLPPLAFYCLLCPEDKAAFDFVLEYMDRMSGYKDWLVENAPGDEVPLGHSLTGFATAFDFLYSSLDNIRRQKYLKKIWAVSEEMYEYSKVRSWGKQLLHNHQATNMLALLTGALVTGVDKESQTNVWKHAVVDMMEKTMFLLNHIVDGSLDEGVAYGSYTAKSVTQYVFLAQRHFGINNLENNWLKMHFWFYYATLLPGYQRTVGIADSNYNWFYGPESQLVFLDKFVLKNGAGNWLAQQIRKHRPKDGPMVPSTAQRWSTLHTEYIWYAAELTPQPPLDYGTAQMHVFPNWGVVTYGAGLPNTQTNTFLSFKSGKLGGRAVYDIVHFQPYSWIDGWRSFNPGHEHPDQNSFTFAPNGQVFVSEALYGPKFSHLNNVLVFAPSPTSQCNQPWEGQLGECAQWLKWTGDEVGDSAGEIITASQYGEMMFVSGEAASAYSSAMKLKSVYRTLLLLNAQTLLVVDHIEKEKDSPLSSVSAFFHNLDIDFKYIPYMFMNKYNGAMMDVWDAHYKMFWFDHHGSSPVARIQEAEQAAEFKKRWTQFINVTFPMKTTVTRIAYVFHGPYVNVSNCRFIDDSKYGFQIFLNVNNTENIVSVVTEYHSLRTRFNYLGFGGFAKVADQNKVIKFGLGTETVQRQTTNNSIVFPFGFKVNIIVGLVLSISLVILAFQWRFYISFSKLLRWILILVITLWFIELMDVWSTCTQPICAKWNSDIINVDPDAGNKAKQSEGNPVVLPDVVITSLPSSGAEILKQLFFNSSDFIYIRIPTTYLDIPETEFEIDSFVDACEWKASDVQSGHFHVLRGWLQSLVQDTKLHLQNIHLHEGSRSKVAQHSIVNKDKKRRARKRESLSEQRSRARGKLDKDAEYVRELRRHLAYYPNARPVLSLSSGSWTLKLPFFQEIIGPSMRALYVVRDPRAWIYSMLHKSKPSLYSLKNVPQHLAMMFKVEDGKEKCSLNSGYAFEYEALREELMDSRSNAVSVLSHLWLANTAAALRVNGYLLQTNYQLVKFEDIVSSPQKTAERIYAFLGIPLSPASLNQILFATSTNLFYLPYEGEISPISIQAWEQDMPVEDIRLIEDICCTVMGHLGYPKFTV

Target ORF information:

RefSeq Version	XM_006118124.3
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis dermatan sulfate epimerase like (DSEL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006118124.3

ORF Insert Sequence:

ATGGCTCTTA TGTTTACACT CCTAGTATTA GCAATGTTTG CGCTCTCCAC TTTTGAAGAA 
TCTGTAAGCA ATTATTCTGA CTGGGTGACT TTTGCAGAAA ATGTGGCTCA ATATGAGAAA 
CAGAAATTAC AAGATTTCAG TGCTGATCAG AAGCTGAAAA AAGTCGTTCT TCATCCAAGC 
TTATATTTTG ATGCTGAAGA TGTCCAAACA CTGAGGCAGA AGTCTCGTAC AAGCCATTTA 
CATCTCTTCA GAGCTATCAG AGGTGCAGTG ACAGTTATGC TGTCCAACCC ATCATACTAC 
CTACCTCCAC CCAAGCATGC TGATTTTGCT GCAAAGTGGA ATGAGATTTA TGGTAACAAT 
CTGCCTCCCT TAGCTTTTTA TTGTTTGCTG TGCCCGGAAG ATAAAGCTGC CTTTGATTTT 
GTTCTAGAGT ACATGGACAG AATGAGTGGC TACAAAGACT GGCTGGTTGA GAATGCTCCA 
GGAGATGAAG TGCCACTTGG CCACTCCTTA ACAGGTTTTG CCACTGCTTT TGACTTCTTA 
TATAGTTCAC TGGATAATAT CAGAAGACAA AAATATCTAA AGAAGATATG GGCTGTGAGT 
GAGGAAATGT ATGAATATTC AAAAGTCCGC TCATGGGGAA AGCAACTTCT CCATAACCAC 
CAAGCCACCA ATATGCTAGC ACTGCTCACG GGGGCCCTGG TTACTGGTGT GGACAAAGAA 
TCTCAGACAA ATGTCTGGAA GCATGCTGTC GTCGATATGA TGGAAAAAAC CATGTTTCTC 
CTCAACCACA TTGTAGATGG CTCTTTGGAT GAAGGAGTCG CATATGGCAG TTACACAGCT 
AAGTCAGTAA CCCAATATGT TTTTCTGGCC CAGCGCCATT TTGGTATTAA TAACCTAGAA 
AACAATTGGT TGAAAATGCA CTTTTGGTTT TATTATGCCA CACTTTTACC CGGCTATCAG 
AGGACTGTGG GCATAGCAGA TTCTAATTAT AACTGGTTTT ATGGCCCTGA AAGCCAGCTG 
GTTTTCCTGG ATAAGTTTGT CCTGAAGAAT GGAGCTGGCA ACTGGTTGGC ACAACAGATT 
AGAAAGCACA GGCCCAAAGA TGGCCCAATG GTACCATCCA CTGCACAAAG GTGGAGCACG 
CTTCACACGG AGTACATATG GTATGCTGCA GAACTAACTC CTCAGCCTCC CCTGGACTAT 
GGCACTGCTC AGATGCATGT GTTTCCTAAC TGGGGGGTTG TTACTTATGG GGCTGGGTTG 
CCGAATACTC AGACCAATAC CTTTCTGTCT TTTAAATCTG GAAAGCTTGG CGGGCGTGCA 
GTCTATGACA TTGTTCACTT TCAGCCATAT TCCTGGATTG ATGGGTGGAG GAGTTTCAAT 
CCAGGGCATG AACATCCAGA TCAGAACTCC TTTACTTTTG CTCCCAATGG ACAGGTGTTT 
GTATCTGAGG CCCTATATGG ACCCAAGTTC AGCCACTTGA ATAACGTACT GGTGTTTGCT 
CCATCTCCTA CAAGTCAGTG TAATCAGCCT TGGGAGGGAC AGCTTGGGGA GTGCGCACAG 
TGGCTTAAGT GGACTGGTGA TGAGGTTGGA GACTCAGCTG GGGAGATAAT AACAGCATCT 
CAATATGGGG AGATGATGTT TGTAAGCGGT GAGGCAGCTT CTGCCTATTC TTCTGCAATG 
AAATTAAAAA GTGTGTATCG TACTTTGCTA CTCCTAAATG CTCAGACACT GCTAGTAGTA 
GACCATATTG AGAAGGAGAA AGACTCTCCC CTCAGCTCTG TCAGTGCCTT CTTTCATAAC 
CTTGACATTG ATTTTAAATA TATACCCTAT ATGTTTATGA ACAAATATAA CGGAGCCATG 
ATGGATGTGT GGGATGCTCA CTATAAGATG TTTTGGTTTG ATCATCACGG GAGTAGTCCT 
GTTGCCAGGA TACAAGAGGC AGAGCAAGCA GCAGAGTTCA AAAAGCGGTG GACTCAGTTC 
ATAAACGTTA CCTTTCCTAT GAAAACCACT GTAACAAGAA TTGCGTATGT GTTCCATGGA 
CCATATGTTA ATGTTTCTAA CTGCAGGTTC ATAGATGATT CCAAATACGG ATTTCAGATT 
TTTCTAAATG TCAACAACAC TGAAAATATT GTGTCTGTTG TGACTGAATA TCACAGTCTG 
AGGACAAGGT TCAATTATTT GGGATTTGGT GGCTTTGCAA AGGTAGCTGA CCAAAACAAA 
GTTATCAAAT TTGGTCTAGG CACTGAAACT GTACAAAGAC AGACAACAAA TAATAGTATC 
GTTTTCCCAT TTGGATTTAA AGTGAATATA ATAGTAGGAT TAGTTTTGAG CATTAGTTTG 
GTAATACTGG CTTTTCAGTG GCGGTTTTAC ATTTCCTTCA GCAAATTGTT GCGCTGGATA 
CTAATATTGG TTATCACACT GTGGTTTATT GAATTGATGG ATGTTTGGAG CACTTGCACT 
CAGCCTATCT GTGCAAAATG GAACAGTGAC ATCATAAACG TAGACCCTGA TGCAGGCAAT 
AAAGCTAAAC AGTCAGAAGG AAACCCTGTT GTTTTGCCAG ATGTTGTGAT TACCTCACTT 
CCCAGTTCTG GAGCAGAAAT TTTAAAGCAG CTTTTTTTTA ACAGCAGTGA TTTCATATAC 
ATCAGGATCC CTACAACTTA CCTTGATATC CCTGAAACTG AATTTGAAAT AGACTCATTT 
GTAGATGCGT GTGAATGGAA GGCGTCTGAT GTCCAGAGTG GCCATTTCCA TGTACTTCGA 
GGCTGGCTCC AGTCTCTAGT CCAAGACACA AAACTTCATT TACAAAATAT TCATTTACAT 
GAAGGCAGCA GAAGCAAAGT TGCTCAGCAT TCGATTGTAA ATAAGGACAA AAAAAGAAGA 
GCCAGAAAGA GAGAATCTTT GTCCGAACAA AGAAGCAGAG CAAGAGGAAA ATTAGACAAA 
GATGCTGAAT ATGTTAGGGA ACTGAGGAGA CACCTTGCCT ATTATCCAAA TGCACGTCCT 
GTGCTTAGTT TAAGTAGTGG GAGCTGGACA TTAAAGCTTC CCTTTTTTCA AGAAATCATA 
GGGCCTTCAA TGAGGGCATT GTATGTAGTA AGGGACCCTA GGGCATGGAT CTACTCAATG 
TTGCATAAGA GTAAGCCAAG CCTTTATTCC TTGAAAAACG TTCCACAGCA CTTAGCAATG 
ATGTTTAAAG TGGAAGACGG GAAAGAAAAA TGCAGTTTAA ATTCAGGCTA TGCCTTTGAA 
TATGAAGCAC TAAGAGAAGA GCTCATGGAT TCCAGGTCAA ATGCAGTTTC TGTGCTGTCC 
CATTTGTGGC TAGCAAACAC AGCAGCAGCA CTGCGAGTAA ATGGGTATCT ACTGCAGACA 
AATTATCAGC TGGTCAAGTT TGAAGATATC GTGAGCTCTC CCCAGAAGAC AGCGGAAAGA 
ATTTATGCTT TTCTTGGTAT TCCTCTGTCT CCTGCTAGCT TAAACCAAAT ATTGTTTGCC 
ACCTCCACCA ATCTTTTCTA TCTTCCTTAT GAGGGTGAAA TATCACCCAT TAGTATTCAA 
GCCTGGGAAC AAGACATGCC TGTTGAAGAT ATTAGACTGA TTGAGGACAT CTGCTGTACT 
GTGATGGGCC ACTTAGGATA CCCAAAGTTT ACAGTTTAA