UBA1 cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBA1
Entrez Gene ID	102447270
Full Name	ubiquitin like modifier activating enzyme 1
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014575492.1	XP_014430978.1	ubiquitin-like modifier-activating enzyme 1
XM_025186646.1	XP_025042431.1	ubiquitin-like modifier-activating enzyme 1

Pathways from BioSystems
Gene Ontology

The following UBA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPe152134	XM_025186646.1 Latest version!	Pelodiscus sinensis ubiquitin like modifier activating enzyme 1 (UBA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPe32947	XM_014575492.1 Latest version!	Pelodiscus sinensis ubiquitin-like modifier activating enzyme 1 (UBA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBA1 ( XM_025186646.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_025042431.1 Pelodiscus sinensis ubiquitin like modifier activating enzyme 1 (UBA1), mRNA.

Price & Availability↑

CloneID	OPe152134
Clone ID Related Accession (Same CDS sequence)	XM_025186646.1
Accession Version	XM_025186646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	ubiquitin-like modifier-activating enzyme 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005856102.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCCAGCT CGCCGTTGTC CAAGAAGCGC CGCGTGTCTG GATCTGAGCC GAAGACGGGT 
TCGAACTGCT CCTCTGGCAA CGCCGTTCCG AGTGACCCAC GCCCAGCGCC CACCAACGGC 
ATGGCTAAGA ATGGGAGCGA CGCCGACATT GATGAGGGCC TGTACTCCAG GCAGCTGTAC 
GTGCTGGGCC ACGAGGCCAT GAAACGGATG CAAAACGCCA GCGTCCTGGT GTCGGGGCTG 
CGGGGGCTGG GCGTGGAGAT CGCCAAGAAC ATCATCCTGG GCGGGGTCAA GTCTGTGACC 
CTGCACGACT CCGCACCCGC CCAGTGGGCA GACCTGGCCT CCCAGTTCTA CCTGCGGGAA 
GACGACATGG GGAAGAACCG GGCCGAGGCC TCCCAGCCCC GCCTGGCTGA GCTCAACTCC 
TATGTGCCCG TGAGCATCCA TACCGGGGCC CTCACCGAGG ACTTCCTCAG CGCCTTCCAG 
GTCGTGGTTC TCACCAGTTC GCCCCTGGAG GAGCAGCTGC GGGTGGGGGA GCTCTGCCAT 
GCCAGAGGCA TCAAGCTGGT GGTGGCTGAC GGGAGCGGGC GCTGGGGAGC AGGCCAGCTC 
TTCTGTGACT TCGGGGACGA GATGGTCGTG ACAGACCCCA ACGGGGAGCA GCCGCTCAGC 
GCCATGGTCT CCATGGTCAC CAAGGGCTGC CCGGGGGAAG TGACGTGCTT GGACGAGGCG 
CGGCACGGCT TTGAGAGCGG GGACTTCGTC TCCTTCACCG AGGTGGAGGG CATGGAGGAG 
CTCAACCACT GCCCCCCCAT GGAGATCCAA GTGCTGGGGC CGTACACCTT CAGCATTGGC 
GACACCAGCA GCTTCTCCGA GTACGTGCGT GGGGGCATCG TCTCCCAGGT CAAGATGCCC 
AAGAAGATCA GCTTTAAACC CCTGAGCGTG GCGCTGGCCC AGCCTGACTT CGTGGTGACC 
GACTTTGGGA AATTCGAGCG CCCGGCCCAG CTGCACTTGG CCTTCCAGGC CCTGCACGAC 
TTCGAGCGCC GGCACCACCG CCTGCCCCGG CCCCGCAGCG AGGTGGACGC AGCCGAGGTG 
CTGGCGCTGA CCCGGGCGCT GAATGAGCAG GCCGCGCCGG CGCTCAAGCA GGAGGAGCTG 
AGCGAGGGGC TGATCAAGGA GCTGGCGTAC CAGGCTGCTG GGGACTTGGC GCCCATCAAC 
GCCTTCATCG GGGGGCTGGC CGCCCAGGAG GTCATGAAGG CCTGCTCTGG GAAGTTCATG 
CCCATCACGC AGTGGCTCTA CTTCGACGCG CTGGAGTGTC TGCCTCAGGA CAGCAAGGAG 
GCGCTGACCG AGGAGGCGTG CCGCCCCGTG AGTGGCTGGG GGCCAGGCGG GGAGCAGTGG 
GCGGAGGTGG GCGCCGGCGC CATCGGCTGC GAGCTGCTGA AGAACTTCGC CATGCTGGGG 
CTGGCCTGCG GGGACGGCGG GGAGGTGACG GTGACCGACA TGGACACCAT CGAGAAATCC 
AACCTCAACC GCCAGTTCCT CTTCCGGCCC TGGGACGTCA CGAAAATGAA GTCTGACACA 
GCGGCAGGTG CCGTGCGTCA GATGAACCCC AGTATCCACA TCACGAGCCA CCAGGACCGT 
GTGGGGCCTG ACACGGAGCG GGTTTACGAC GACGACTTCT TCGAGGGCCT GGACGGTGTG 
GCCAATGCTC TGGACAACGT GGATGCCAGG ATGTACATGG ATCGCCGGTG CGTCTATTAC 
CGCAAACCGC TGCTGGAGTC AGGGACGCTG GGCACCAAGG GCAACGTCCA GGTGGTGATC 
CCCTTCCTGA CGGAGTCCTA CAGCTCCAGC CAGGACCCGC CCGAGAAATC ACAGGCCAAG 
GCTCTGGTGC GTCAGGCAGC TCTATTACTA CACCGGCAGG AGGAGCTGAA ATCCAGCCTG 
CCCAGCCCCG AGGGCCTGCA GAGCTTCCGC ATGTACCCCA TCGACTTCGA GAAGGTTGCG 
ACGACGACGG CGGCCGTGGT GGGGCTGGTG TGTCTGGAGC TGTACAAGGT GGTGCAGGGT 
CACAAGCGGC TGGAGTCCTA CAAGAACGGC TTCCTCAACC TGGCGCTGCC CTTCTTCGGC 
TTCTCCGAGC CCATCGCCTG CCCCCGCAAC AAGGTGGGTG CCGCCGTGTG CACCCTGGGC 
CCCTGGGACC GGTTCGAGGT GCAGGGGGTG CAGCCCAGTG GGGAGGAGAT GACGCTGCGC 
CAGTTCCTGG CCTACTTCAA GACAGAGCAC CGGCTGGAGA TCACCATGCT GTCGCAGGGC 
GTCTCCATGC TCTACTCCTT CTTCATGCCG CCTGCTAAGC TGCGCGAGCG CCACGACCAG 
CCGATGACGG AAATCGTGAC CAAGGTGTCG AAGAAGAAGA TCGGGCGTCA TGTCAAGGCC 
CTGGTCTTCG AACTCTGCTG TAACGACGAC AGCGAGTGCG ACATCGCGCC AGGGGCGGGG 
CCTCCCCACG CCCCGCCCCC GAGCTCGTGG GGCGGGGTCT CCCACGCCCC GCCCACCGGT 
TACAGTGATG GAAAACATGG ACTAAGGGGG ATTCCCCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025042431.1
CDS	192..2753
Translation	MSSSPLSKKRRVSGSEPKTGSNCSSGNAVPSDPRPAPTNGMAKNGSDADIDEGLYSRQLYVLGHEAMKRMQNASVLVSGLRGLGVEIAKNIILGGVKSVTLHDSAPAQWADLASQFYLREDDMGKNRAEASQPRLAELNSYVPVSIHTGALTEDFLSAFQVVVLTSSPLEEQLRVGELCHARGIKLVVADGSGRWGAGQLFCDFGDEMVVTDPNGEQPLSAMVSMVTKGCPGEVTCLDEARHGFESGDFVSFTEVEGMEELNHCPPMEIQVLGPYTFSIGDTSSFSEYVRGGIVSQVKMPKKISFKPLSVALAQPDFVVTDFGKFERPAQLHLAFQALHDFERRHHRLPRPRSEVDAAEVLALTRALNEQAAPALKQEELSEGLIKELAYQAAGDLAPINAFIGGLAAQEVMKACSGKFMPITQWLYFDALECLPQDSKEALTEEACRPVSGWGPGGEQWAEVGAGAIGCELLKNFAMLGLACGDGGEVTVTDMDTIEKSNLNRQFLFRPWDVTKMKSDTAAGAVRQMNPSIHITSHQDRVGPDTERVYDDDFFEGLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSQAKALVRQAALLLHRQEELKSSLPSPEGLQSFRMYPIDFEKVATTTAAVVGLVCLELYKVVQGHKRLESYKNGFLNLALPFFGFSEPIACPRNKVGAAVCTLGPWDRFEVQGVQPSGEEMTLRQFLAYFKTEHRLEITMLSQGVSMLYSFFMPPAKLRERHDQPMTEIVTKVSKKKIGRHVKALVFELCCNDDSECDIAPGAGPPHAPPPSSWGGVSHAPPTGYSDGKHGLRGIPP

Target ORF information:

RefSeq Version	XM_025186646.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis ubiquitin like modifier activating enzyme 1 (UBA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025186646.1

ORF Insert Sequence:

ATGTCCAGCT CGCCGTTGTC CAAGAAGCGC CGCGTGTCTG GATCTGAGCC GAAGACGGGT 
TCGAACTGCT CCTCTGGCAA CGCCGTTCCG AGTGACCCAC GCCCAGCGCC CACCAACGGC 
ATGGCTAAGA ATGGGAGCGA CGCCGACATT GATGAGGGCC TGTACTCCAG GCAGCTGTAC 
GTGCTGGGCC ACGAGGCCAT GAAACGGATG CAAAACGCCA GCGTCCTGGT GTCGGGGCTG 
CGGGGGCTGG GCGTGGAGAT CGCCAAGAAC ATCATCCTGG GCGGGGTCAA GTCTGTGACC 
CTGCACGACT CCGCACCCGC CCAGTGGGCA GACCTGGCCT CCCAGTTCTA CCTGCGGGAA 
GACGACATGG GGAAGAACCG GGCCGAGGCC TCCCAGCCCC GCCTGGCTGA GCTCAACTCC 
TATGTGCCCG TGAGCATCCA TACCGGGGCC CTCACCGAGG ACTTCCTCAG CGCCTTCCAG 
GTCGTGGTTC TCACCAGTTC GCCCCTGGAG GAGCAGCTGC GGGTGGGGGA GCTCTGCCAT 
GCCAGAGGCA TCAAGCTGGT GGTGGCTGAC GGGAGCGGGC GCTGGGGAGC AGGCCAGCTC 
TTCTGTGACT TCGGGGACGA GATGGTCGTG ACAGACCCCA ACGGGGAGCA GCCGCTCAGC 
GCCATGGTCT CCATGGTCAC CAAGGGCTGC CCGGGGGAAG TGACGTGCTT GGACGAGGCG 
CGGCACGGCT TTGAGAGCGG GGACTTCGTC TCCTTCACCG AGGTGGAGGG CATGGAGGAG 
CTCAACCACT GCCCCCCCAT GGAGATCCAA GTGCTGGGGC CGTACACCTT CAGCATTGGC 
GACACCAGCA GCTTCTCCGA GTACGTGCGT GGGGGCATCG TCTCCCAGGT CAAGATGCCC 
AAGAAGATCA GCTTTAAACC CCTGAGCGTG GCGCTGGCCC AGCCTGACTT CGTGGTGACC 
GACTTTGGGA AATTCGAGCG CCCGGCCCAG CTGCACTTGG CCTTCCAGGC CCTGCACGAC 
TTCGAGCGCC GGCACCACCG CCTGCCCCGG CCCCGCAGCG AGGTGGACGC AGCCGAGGTG 
CTGGCGCTGA CCCGGGCGCT GAATGAGCAG GCCGCGCCGG CGCTCAAGCA GGAGGAGCTG 
AGCGAGGGGC TGATCAAGGA GCTGGCGTAC CAGGCTGCTG GGGACTTGGC GCCCATCAAC 
GCCTTCATCG GGGGGCTGGC CGCCCAGGAG GTCATGAAGG CCTGCTCTGG GAAGTTCATG 
CCCATCACGC AGTGGCTCTA CTTCGACGCG CTGGAGTGTC TGCCTCAGGA CAGCAAGGAG 
GCGCTGACCG AGGAGGCGTG CCGCCCCGTG AGTGGCTGGG GGCCAGGCGG GGAGCAGTGG 
GCGGAGGTGG GCGCCGGCGC CATCGGCTGC GAGCTGCTGA AGAACTTCGC CATGCTGGGG 
CTGGCCTGCG GGGACGGCGG GGAGGTGACG GTGACCGACA TGGACACCAT CGAGAAATCC 
AACCTCAACC GCCAGTTCCT CTTCCGGCCC TGGGACGTCA CGAAAATGAA GTCTGACACA 
GCGGCAGGTG CCGTGCGTCA GATGAACCCC AGTATCCACA TCACGAGCCA CCAGGACCGT 
GTGGGGCCTG ACACGGAGCG GGTTTACGAC GACGACTTCT TCGAGGGCCT GGACGGTGTG 
GCCAATGCTC TGGACAACGT GGATGCCAGG ATGTACATGG ATCGCCGGTG CGTCTATTAC 
CGCAAACCGC TGCTGGAGTC AGGGACGCTG GGCACCAAGG GCAACGTCCA GGTGGTGATC 
CCCTTCCTGA CGGAGTCCTA CAGCTCCAGC CAGGACCCGC CCGAGAAATC ACAGGCCAAG 
GCTCTGGTGC GTCAGGCAGC TCTATTACTA CACCGGCAGG AGGAGCTGAA ATCCAGCCTG 
CCCAGCCCCG AGGGCCTGCA GAGCTTCCGC ATGTACCCCA TCGACTTCGA GAAGGTTGCG 
ACGACGACGG CGGCCGTGGT GGGGCTGGTG TGTCTGGAGC TGTACAAGGT GGTGCAGGGT 
CACAAGCGGC TGGAGTCCTA CAAGAACGGC TTCCTCAACC TGGCGCTGCC CTTCTTCGGC 
TTCTCCGAGC CCATCGCCTG CCCCCGCAAC AAGGTGGGTG CCGCCGTGTG CACCCTGGGC 
CCCTGGGACC GGTTCGAGGT GCAGGGGGTG CAGCCCAGTG GGGAGGAGAT GACGCTGCGC 
CAGTTCCTGG CCTACTTCAA GACAGAGCAC CGGCTGGAGA TCACCATGCT GTCGCAGGGC 
GTCTCCATGC TCTACTCCTT CTTCATGCCG CCTGCTAAGC TGCGCGAGCG CCACGACCAG 
CCGATGACGG AAATCGTGAC CAAGGTGTCG AAGAAGAAGA TCGGGCGTCA TGTCAAGGCC 
CTGGTCTTCG AACTCTGCTG TAACGACGAC AGCGAGTGCG ACATCGCGCC AGGGGCGGGG 
CCTCCCCACG CCCCGCCCCC GAGCTCGTGG GGCGGGGTCT CCCACGCCCC GCCCACCGGT 
TACAGTGATG GAAAACATGG ACTAAGGGGG ATTCCCCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA1 ( XM_014575492.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_014430978.1 Pelodiscus sinensis ubiquitin-like modifier activating enzyme 1 (UBA1), mRNA.

Price & Availability↑

CloneID	OPe32947
Clone ID Related Accession (Same CDS sequence)	XM_014575492.1
Accession Version	XM_014575492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	ubiquitin-like modifier-activating enzyme 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005856102.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCCAGCT CGCCGTTGTC CAAGAAGCGC CGCGTGTCTG GATCTGAGCC GAAGACGGGT 
TCGAACTGCT CCTCTGGCAA CGCCGTTCCG AGTGACCCAC GCCCAGCGCC CACCAACGGC 
ATGGCTAAGA ATGGGAGCGA CGCCGACATT GATGAGGGCC TGTACTCCAG GCAGCTGTAC 
GTGCTGGGCC ACGAGGCCAT GAAACGGATG CAAAACGCCA GCGTCCTGGT GTCGGGGCTG 
CGGGGGCTGG GCGTGGAGAT CGCCAAGAAC ATCATCCTGG GCGGGGTCAA GTCTGTGACC 
CTGCACGACT CCGCACCCGC CCAGTGGGCA GACCTGGCCT CCCAGTTCTA CCTGCGGGAA 
GACGACATGG GGAAGAACCG GGCCGAGGCC TCCCAGCCCC GCCTGGCTGA GCTCAACTCC 
TATGTGCCCG TGAGCATCCA TACCGGGGCC CTCACCGAGG ACTTCCTCAG CGCCTTCCAG 
GTCGTGGTTC TCACCAGTTC GCCCCTGGAG GAGCAGCTGC GGGTGGGGGA GCTCTGCCAT 
GCCAGAGGCA TCAAGCTGGT GGTGGCTGAC GGGAGCGGGC GCTGGGGAGC AGGCCAGCTC 
TTCTGTGACT TCGGGGACGA GATGGTCGTG ACAGACCCCA ACGGGGAGCA GCCGCTCAGC 
GCCATGGTCT CCATGGTCAC CAAGGGCTGC CCGGGGGAAG TGACGTGCTT GGACGAGGCG 
CGGCACGGCT TTGAGAGCGG GGACTTCGTC TCCTTCACCG AGGTGGAGGG CATGGAGGAG 
CTCAACCACT GCCCCCCCAT GGAGATCCAA GTGCTGGGGC CGTACACCTT CAGCATTGGC 
GACACCAGCA GCTTCTCCGA GTACGTGCGT GGGGGCATCG TCTCCCAGGT CAAGATGCCC 
AAGAAGATCA GCTTTAAACC CCTGAGCGTG GCGCTGGCCC AGCCTGACTT CGTGGTGACC 
GACTTTGGGA AATTCGAGCG CCCGGCCCAG CTGCACTTGG CCTTCCAGGC CCTGCACGAC 
TTCGAGCGCC GGCACCACCG CCTGCCCCGG CCCCGCAGCG AGGTGGACGC AGCCGAGGTG 
CTGGCGCTGA CCCGGGCGCT GAATGAGCAG GCCGCGCCGG CGCTCAAGCA GGAGGAGCTG 
AGCGAGGGGC TGATCAAGGA GCTGGCGTAC CAGGCTGCTG GGGACTTGGC GCCCATCAAC 
GCCTTCATCG GGGGGCTGGC CGCCCAGGAG GTCATGAAGG CCTGCTCTGG GAAGTTCATG 
CCCATCACGC AGTGGCTCTA CTTCGACGCG CTGGAGTGTC TGCCTCAGGA CAGCAAGGAG 
GCGCTGACCG AGGAGGCGTG CCGCCCCGTG AGTGGCTGGG GGCCAGGCGG GGAGCAGTGG 
GCGGAGGTGG GCGCCGGCGC CATCGGCTGC GAGCTGCTGA AGAACTTCGC CATGCTGGGG 
CTGGCCTGCG GGGACGGCGG GGAGGTGACG GTGACCGACA TGGACACCAT CGAGAAATCC 
AACCTCAACC GCCAGTTCCT CTTCCGGCCC TGGGACGTCA CGAAAATGAA GTCTGACACA 
GCGGCAGGTG CCGTGCGTCA GATGAACCCC AGTATCCACA TCACGAGCCA CCAGGACCGT 
GTGGGGCCTG ACACGGAGCG GGTTTACGAC GACGACTTCT TCGAGGGCCT GGACGGTGTG 
GCCAATGCTC TGGACAACGT GGATGCCAGG ATGTACATGG ATCGCCGGTG CGTCTATTAC 
CGCAAACCGC TGCTGGAGTC AGGGACGCTG GGCACCAAGG GCAACGTCCA GGTGGTGATC 
CCCTTCCTGA CGGAGTCCTA CAGCTCCAGC CAGGACCCGC CCGAGAAATC ACAGGCCAAG 
GCTCTGGTGC GTCAGGCAGC TCTATTACTA CACCGGCAGG AGGAGCTGAA ATCCAGCCTG 
CCCAGCCCCG AGGGCCTGCA GAGCTTCCGC ATGTACCCCA TCGACTTCGA GAAGGTGATT 
GCGACGACGA CGGCGGCCGT GGTGGGGCTG GTGTGTCTGG AGCTGTACAA GGTGGTGCAG 
GGTCACAAGC GGCTGGAGTC CTACAAGAAC GGCTTCCTCA ACCTGGCGCT GCCCTTCTTC 
GGCTTCTCCG AGCCCATCGC CTGCCCCCGC AACAAGGTGG GTGCCGCCGT GTGCACCCTG 
GGCCCCTGGG ACCGGTTCGA GGTGCAGGGG GTGCAGCCCA GTGGGGAGGA GATGACGCTG 
CGCCAGTTCC TGGCCTACTT CAAGACAGAG CACCGGCTGG AGATCACCAT GCTGTCGCAG 
GGCGTCTCCA TGCTCTACTC CTTCTTCATG CCGCCTGCTA AGCTGCGCGA GCGCCACGAC 
CAGCCGATGA CGGAAATCGT GACCAAGGTG TCGAAGAAGA AGATCGGGCG TCATGTCAAG 
GCCCTGGTCT TCGAACTCTG CTGTAACGAC GACAGCGAGT GCGACATCGC GCCAGGGGCG 
GGGCCTCCCC ACGCCCCGCC CCCGAGCTCG TGGGGCGGGG TCTCCCACGC CCCGCCCACC 
GGTTACAGTG ATGGAAAACA TGGACTAAGG GGGATTCCCC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014430978.1
CDS	190..2754
Translation	MSSSPLSKKRRVSGSEPKTGSNCSSGNAVPSDPRPAPTNGMAKNGSDADIDEGLYSRQLYVLGHEAMKRMQNASVLVSGLRGLGVEIAKNIILGGVKSVTLHDSAPAQWADLASQFYLREDDMGKNRAEASQPRLAELNSYVPVSIHTGALTEDFLSAFQVVVLTSSPLEEQLRVGELCHARGIKLVVADGSGRWGAGQLFCDFGDEMVVTDPNGEQPLSAMVSMVTKGCPGEVTCLDEARHGFESGDFVSFTEVEGMEELNHCPPMEIQVLGPYTFSIGDTSSFSEYVRGGIVSQVKMPKKISFKPLSVALAQPDFVVTDFGKFERPAQLHLAFQALHDFERRHHRLPRPRSEVDAAEVLALTRALNEQAAPALKQEELSEGLIKELAYQAAGDLAPINAFIGGLAAQEVMKACSGKFMPITQWLYFDALECLPQDSKEALTEEACRPVSGWGPGGEQWAEVGAGAIGCELLKNFAMLGLACGDGGEVTVTDMDTIEKSNLNRQFLFRPWDVTKMKSDTAAGAVRQMNPSIHITSHQDRVGPDTERVYDDDFFEGLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSQAKALVRQAALLLHRQEELKSSLPSPEGLQSFRMYPIDFEKVIATTTAAVVGLVCLELYKVVQGHKRLESYKNGFLNLALPFFGFSEPIACPRNKVGAAVCTLGPWDRFEVQGVQPSGEEMTLRQFLAYFKTEHRLEITMLSQGVSMLYSFFMPPAKLRERHDQPMTEIVTKVSKKKIGRHVKALVFELCCNDDSECDIAPGAGPPHAPPPSSWGGVSHAPPTGYSDGKHGLRGIPP

Target ORF information:

RefSeq Version	XM_014575492.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis ubiquitin-like modifier activating enzyme 1 (UBA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014575492.1

ORF Insert Sequence:

ATGTCCAGCT CGCCGTTGTC CAAGAAGCGC CGCGTGTCTG GATCTGAGCC GAAGACGGGT 
TCGAACTGCT CCTCTGGCAA CGCCGTTCCG AGTGACCCAC GCCCAGCGCC CACCAACGGC 
ATGGCTAAGA ATGGGAGCGA CGCCGACATT GATGAGGGCC TGTACTCCAG GCAGCTGTAC 
GTGCTGGGCC ACGAGGCCAT GAAACGGATG CAAAACGCCA GCGTCCTGGT GTCGGGGCTG 
CGGGGGCTGG GCGTGGAGAT CGCCAAGAAC ATCATCCTGG GCGGGGTCAA GTCTGTGACC 
CTGCACGACT CCGCACCCGC CCAGTGGGCA GACCTGGCCT CCCAGTTCTA CCTGCGGGAA 
GACGACATGG GGAAGAACCG GGCCGAGGCC TCCCAGCCCC GCCTGGCTGA GCTCAACTCC 
TATGTGCCCG TGAGCATCCA TACCGGGGCC CTCACCGAGG ACTTCCTCAG CGCCTTCCAG 
GTCGTGGTTC TCACCAGTTC GCCCCTGGAG GAGCAGCTGC GGGTGGGGGA GCTCTGCCAT 
GCCAGAGGCA TCAAGCTGGT GGTGGCTGAC GGGAGCGGGC GCTGGGGAGC AGGCCAGCTC 
TTCTGTGACT TCGGGGACGA GATGGTCGTG ACAGACCCCA ACGGGGAGCA GCCGCTCAGC 
GCCATGGTCT CCATGGTCAC CAAGGGCTGC CCGGGGGAAG TGACGTGCTT GGACGAGGCG 
CGGCACGGCT TTGAGAGCGG GGACTTCGTC TCCTTCACCG AGGTGGAGGG CATGGAGGAG 
CTCAACCACT GCCCCCCCAT GGAGATCCAA GTGCTGGGGC CGTACACCTT CAGCATTGGC 
GACACCAGCA GCTTCTCCGA GTACGTGCGT GGGGGCATCG TCTCCCAGGT CAAGATGCCC 
AAGAAGATCA GCTTTAAACC CCTGAGCGTG GCGCTGGCCC AGCCTGACTT CGTGGTGACC 
GACTTTGGGA AATTCGAGCG CCCGGCCCAG CTGCACTTGG CCTTCCAGGC CCTGCACGAC 
TTCGAGCGCC GGCACCACCG CCTGCCCCGG CCCCGCAGCG AGGTGGACGC AGCCGAGGTG 
CTGGCGCTGA CCCGGGCGCT GAATGAGCAG GCCGCGCCGG CGCTCAAGCA GGAGGAGCTG 
AGCGAGGGGC TGATCAAGGA GCTGGCGTAC CAGGCTGCTG GGGACTTGGC GCCCATCAAC 
GCCTTCATCG GGGGGCTGGC CGCCCAGGAG GTCATGAAGG CCTGCTCTGG GAAGTTCATG 
CCCATCACGC AGTGGCTCTA CTTCGACGCG CTGGAGTGTC TGCCTCAGGA CAGCAAGGAG 
GCGCTGACCG AGGAGGCGTG CCGCCCCGTG AGTGGCTGGG GGCCAGGCGG GGAGCAGTGG 
GCGGAGGTGG GCGCCGGCGC CATCGGCTGC GAGCTGCTGA AGAACTTCGC CATGCTGGGG 
CTGGCCTGCG GGGACGGCGG GGAGGTGACG GTGACCGACA TGGACACCAT CGAGAAATCC 
AACCTCAACC GCCAGTTCCT CTTCCGGCCC TGGGACGTCA CGAAAATGAA GTCTGACACA 
GCGGCAGGTG CCGTGCGTCA GATGAACCCC AGTATCCACA TCACGAGCCA CCAGGACCGT 
GTGGGGCCTG ACACGGAGCG GGTTTACGAC GACGACTTCT TCGAGGGCCT GGACGGTGTG 
GCCAATGCTC TGGACAACGT GGATGCCAGG ATGTACATGG ATCGCCGGTG CGTCTATTAC 
CGCAAACCGC TGCTGGAGTC AGGGACGCTG GGCACCAAGG GCAACGTCCA GGTGGTGATC 
CCCTTCCTGA CGGAGTCCTA CAGCTCCAGC CAGGACCCGC CCGAGAAATC ACAGGCCAAG 
GCTCTGGTGC GTCAGGCAGC TCTATTACTA CACCGGCAGG AGGAGCTGAA ATCCAGCCTG 
CCCAGCCCCG AGGGCCTGCA GAGCTTCCGC ATGTACCCCA TCGACTTCGA GAAGGTGATT 
GCGACGACGA CGGCGGCCGT GGTGGGGCTG GTGTGTCTGG AGCTGTACAA GGTGGTGCAG 
GGTCACAAGC GGCTGGAGTC CTACAAGAAC GGCTTCCTCA ACCTGGCGCT GCCCTTCTTC 
GGCTTCTCCG AGCCCATCGC CTGCCCCCGC AACAAGGTGG GTGCCGCCGT GTGCACCCTG 
GGCCCCTGGG ACCGGTTCGA GGTGCAGGGG GTGCAGCCCA GTGGGGAGGA GATGACGCTG 
CGCCAGTTCC TGGCCTACTT CAAGACAGAG CACCGGCTGG AGATCACCAT GCTGTCGCAG 
GGCGTCTCCA TGCTCTACTC CTTCTTCATG CCGCCTGCTA AGCTGCGCGA GCGCCACGAC 
CAGCCGATGA CGGAAATCGT GACCAAGGTG TCGAAGAAGA AGATCGGGCG TCATGTCAAG 
GCCCTGGTCT TCGAACTCTG CTGTAACGAC GACAGCGAGT GCGACATCGC GCCAGGGGCG 
GGGCCTCCCC ACGCCCCGCC CCCGAGCTCG TGGGGCGGGG TCTCCCACGC CCCGCCCACC 
GGTTACAGTG ATGGAAAACA TGGACTAAGG GGGATTCCCC CATAG