VPS18 cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS18
Entrez Gene ID	102447191
Full Name	VPS18, CORVET/HOPS core subunit
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025187525.1	XP_025043310.1	vacuolar protein sorting-associated protein 18 homolog isoform X1
XM_006128686.1	XP_006128748.1	vacuolar protein sorting-associated protein 18 homolog isoform X2

Pathways from BioSystems
Gene Ontology

The following VPS18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPe152720	XM_025187525.1 Latest version!	Pelodiscus sinensis VPS18, CORVET/HOPS core subunit (VPS18), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPe34438	XM_006128686.1 Latest version!	Pelodiscus sinensis VPS18, CORVET/HOPS core subunit (VPS18), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS18 ( XM_025187525.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_025043310.1 Pelodiscus sinensis VPS18, CORVET/HOPS core subunit (VPS18), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPe152720
Clone ID Related Accession (Same CDS sequence)	XM_025187525.1
Accession Version	XM_025187525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	vacuolar protein sorting-associated protein 18 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005856687.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGCT CCTCATGGGG CTGGAGCAAT GCAGGGGAGA GCTGCTCCAG CCCCCATCTG 
TTAACTGCAA CCTTTCACGT CCCTAGTTTC TGCCAAATTG TCTGTAAAAT TGGAGGATTT 
GTGAATGCTC GGCTGGAGAA GGAGACACCA ATATTTAACA AGCAAAGGAT TGATTTCACT 
CCTCCAGAGA AAATTAACAG TCTAGTGGTC TCCTCTAATC AGCTTTGTAT GAGCCTTGGC 
AGAGACACCC TTTTCAGGAT TGATCTTGGC AAGGCAGATG AACCTAATCA GGTGGAGTTG 
GGACGCAAGG ATGAAGCCAG AGTCTACAAG ATGTTTTTAG ATCACACAGG ATCTCACCTT 
GTGATTGCAC TGAACACTAG TGAATGCCTG TATTTGAACA GAAGCGCTCA GAAGGTGCGG 
GCGCTCTCCC GCTGGAAAGG TCACTTGGTT GAATGTGTGG GCTGGAATAA GTTCCTGGGT 
TCAGAGACGA ACACTGGTCC TATCCTGGTG GGAACAGCCC AGGGCCAAAT ATACGAGGCT 
GAGATCTCTG TCAGCGAGGG AAGCTTCTTC AGCACTAATC CAGACCAGTA TTTCCGTCTG 
GTCTATACCT TGGAGGAGGA GGCAGGGCCC ACCCCAGTCT GCTGTCTGGA AATAGAGAGA 
GTGCTCGATG GGAAATGTTT CATCATTGCC ACTACTTGTA AAAGACTCTT CCAGTTTGCG 
GGCAAAGCGC TTGAGGGGAC AGAGCAGCAG GGCTTTGGCT TTGTGTTTGG CATGCATGTA 
GACCACTTGC CCAGCTTTCG AGAGTTCCCA GCCAGCTTTG GCTACAGCGA AATAGCATTT 
TATACCCCAA AGTTGCGCTC CTCCCCACGG TCCTTTGCCT GGATGATGGG AAATGGTGTC 
TTGTATGGTA CGTTGGACTA TGGACGGCCT GACTCTATTC TGAGTGATGA ACGGGTTTGG 
GAGTATCCTT CTGACGTGGA TGTGTCAGCT AACAAGCCGA TTTCCATAGT TCTCACCCAA 
TTCCACTTCC TGCTGTTACT GCCTGACAAA GTGATAGCTG TGTGCACTCT GAATGGGCAG 
GTTGTCTTCC AAGATGTGTT CTTGGAGAAA TTTGGCTCTT TGAAGCGCAT GATCAAAGAT 
CCCACAGTCG GGCAGGTCTG GATCCACACA GAGAAAGTTG TATTCCGCTA CCACGTACAA 
CGGGAATCGA GAGATGTATG GAAGATGTAT ATGAACATTA ACAAGTTTGA TCTGGCCAAA 
GAGTACTGCA AAGACCGTCC TGAGTGCCTA GATATTGTGT TGGCAAGAGA GGCAGAACAC 
TCCTTTCAAA ACAAGAGGTA CTTGGATAGT GCCAAGTGTT ATGCACTGAC CCAGAATTAC 
TTTGAGGAGA TTGCCCTTAA GTTCATTGAA GCCAAACAAG AGGAGGCATT GATGGAGTTT 
CTGATTAAGA AACTGGCCAA CTTAAAGTCT TCTGAAAAGA CCCAGACAAC CTTGTTGACC 
ACCTGGTTAA CAGAGCTCTA CTTGAATCGG CTAGGTGCTT TGGAGGGTGA CGCCTCCAAA 
AGGAATCTCT ATGAGGAGAC TCGGGAGAAG TTCTGCAGCT TCCTGAGCAG CCCCAAAAAC 
AAAGAGTGTC TGTTCAATAA CCGAGTGTCC ATCTATGAGC TGCTAGCAAG CCACGGGGAC 
ACAGAACACA TGGTCTATTT TTCAGTTATT ATGCAAGACT ACGAGAGAGT GGTAGCCCAT 
CACTGCCAAC ATGATGACTA TGATGAAGCC TTAAATGTTC TGTCCAGACA CAGGGATGAA 
AAGCTCTTTT ACAAGTTCTC TCCGGTCCTG ATCCAGCACA TCCCCAAGAA GGTGGTTGAT 
GCTTGGATCT CCATGGGCAC AAAACTGGAT GCCAGGAAGC TCATACCTGC CCTCGTCAAC 
TACAGTCAAA GTGAAAGCAC CCAACAGATC AGTGAAGCTA TTCGATACAT GGAGTTCTGT 
GTGTGTGAGC TGGAAGAAAC GGAGCAAGCC ATTCACAACT ATCTGTTGTC TCTCTATGCT 
TTGTGTCGGC CAGTCTCGCT GCTGTCGTAC CTAGAACAAG CAGGAACCAA CCCTAACAGG 
ATCCACTATG ACCTGAAGTA TGCGCTTCGG CTGTGTGCAG AGCACGGACA CCACCGAGCC 
TGTGTCCATG TTTACAAAGT GATGGAGTTA TATGAGGAAG CAGTGGATCT AGCTTTGCAG 
GTGGATGTGG ATTTGGCTAA GTCCTGTGCC GACCTCCCTG AAGAGGATGA GGAACTCCGG 
AAGAAGCTCT GGTTGAAAAT TGCTCGTCAT GTAGTCCAGG AAGAGAAGGA TGTAAAGAAA 
GCCATGGCCT GCCTTTCTAG CTGCAACCTG CTGAAGATTG AAGATGTCTT GCCCTTTTTC 
CCTGACTTTG TCACCATTGA CCACTTTAAA GAGGCTATCT GCAACTCTCT GGAGGACTAC 
AACAAGCACA TTGAGGAGCT GAAAAGAGAG ATGGAGGAAG CCACGCAGAG TGCCAAGAGA 
ATTCGGGAGG ACATCCAGGA GATGAGAAAC AAATACGGAT CTGTGGAGCC TCAGGAAAAG 
TGTGCAGCCT GTGACTTTCC GCTCCTAAAT CGCCCTTTCT ACCTTTTTCT TTGTGGTCAC 
ATGTTCCATT ATGACTGCCT GTTCCAGGCA GTTTTTCCAA ACCTGCCTGC CTGGAAGCAG 
GCTAAGCTAG AAGACCTTCA GAAGAAGCTG GGAGCCACAA GCCAGCCCTC CAAAGCCCAT 
CATCGTCCCA AGGACATAGA TGCTGCTAGC TTGGGAAAAG GGCAGCAGAG TAGGGAACAG 
ATCAAGGCAG ACATTGATGA TATTGTGGCG GCTGAATGTG TGTATTGTGG GGAGTTGATG 
ATCCGGTCAA TTGACAAGCC TTTCATTGAC CCACAAAAGT ATGAAGAGGA GAGACAAAGT 
TGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025043310.1
CDS	98..3046
Translation	MVGSSWGWSNAGESCSSPHLLTATFHVPSFCQIVCKIGGFVNARLEKETPIFNKQRIDFTPPEKINSLVVSSNQLCMSLGRDTLFRIDLGKADEPNQVELGRKDEARVYKMFLDHTGSHLVIALNTSECLYLNRSAQKVRALSRWKGHLVECVGWNKFLGSETNTGPILVGTAQGQIYEAEISVSEGSFFSTNPDQYFRLVYTLEEEAGPTPVCCLEIERVLDGKCFIIATTCKRLFQFAGKALEGTEQQGFGFVFGMHVDHLPSFREFPASFGYSEIAFYTPKLRSSPRSFAWMMGNGVLYGTLDYGRPDSILSDERVWEYPSDVDVSANKPISIVLTQFHFLLLLPDKVIAVCTLNGQVVFQDVFLEKFGSLKRMIKDPTVGQVWIHTEKVVFRYHVQRESRDVWKMYMNINKFDLAKEYCKDRPECLDIVLAREAEHSFQNKRYLDSAKCYALTQNYFEEIALKFIEAKQEEALMEFLIKKLANLKSSEKTQTTLLTTWLTELYLNRLGALEGDASKRNLYEETREKFCSFLSSPKNKECLFNNRVSIYELLASHGDTEHMVYFSVIMQDYERVVAHHCQHDDYDEALNVLSRHRDEKLFYKFSPVLIQHIPKKVVDAWISMGTKLDARKLIPALVNYSQSESTQQISEAIRYMEFCVCELEETEQAIHNYLLSLYALCRPVSLLSYLEQAGTNPNRIHYDLKYALRLCAEHGHHRACVHVYKVMELYEEAVDLALQVDVDLAKSCADLPEEDEELRKKLWLKIARHVVQEEKDVKKAMACLSSCNLLKIEDVLPFFPDFVTIDHFKEAICNSLEDYNKHIEELKREMEEATQSAKRIREDIQEMRNKYGSVEPQEKCAACDFPLLNRPFYLFLCGHMFHYDCLFQAVFPNLPAWKQAKLEDLQKKLGATSQPSKAHHRPKDIDAASLGKGQQSREQIKADIDDIVAAECVYCGELMIRSIDKPFIDPQKYEEERQSWL

Target ORF information:

RefSeq Version	XM_025187525.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis VPS18, CORVET/HOPS core subunit (VPS18), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025187525.1

ORF Insert Sequence:

ATGGTGGGCT CCTCATGGGG CTGGAGCAAT GCAGGGGAGA GCTGCTCCAG CCCCCATCTG 
TTAACTGCAA CCTTTCACGT CCCTAGTTTC TGCCAAATTG TCTGTAAAAT TGGAGGATTT 
GTGAATGCTC GGCTGGAGAA GGAGACACCA ATATTTAACA AGCAAAGGAT TGATTTCACT 
CCTCCAGAGA AAATTAACAG TCTAGTGGTC TCCTCTAATC AGCTTTGTAT GAGCCTTGGC 
AGAGACACCC TTTTCAGGAT TGATCTTGGC AAGGCAGATG AACCTAATCA GGTGGAGTTG 
GGACGCAAGG ATGAAGCCAG AGTCTACAAG ATGTTTTTAG ATCACACAGG ATCTCACCTT 
GTGATTGCAC TGAACACTAG TGAATGCCTG TATTTGAACA GAAGCGCTCA GAAGGTGCGG 
GCGCTCTCCC GCTGGAAAGG TCACTTGGTT GAATGTGTGG GCTGGAATAA GTTCCTGGGT 
TCAGAGACGA ACACTGGTCC TATCCTGGTG GGAACAGCCC AGGGCCAAAT ATACGAGGCT 
GAGATCTCTG TCAGCGAGGG AAGCTTCTTC AGCACTAATC CAGACCAGTA TTTCCGTCTG 
GTCTATACCT TGGAGGAGGA GGCAGGGCCC ACCCCAGTCT GCTGTCTGGA AATAGAGAGA 
GTGCTCGATG GGAAATGTTT CATCATTGCC ACTACTTGTA AAAGACTCTT CCAGTTTGCG 
GGCAAAGCGC TTGAGGGGAC AGAGCAGCAG GGCTTTGGCT TTGTGTTTGG CATGCATGTA 
GACCACTTGC CCAGCTTTCG AGAGTTCCCA GCCAGCTTTG GCTACAGCGA AATAGCATTT 
TATACCCCAA AGTTGCGCTC CTCCCCACGG TCCTTTGCCT GGATGATGGG AAATGGTGTC 
TTGTATGGTA CGTTGGACTA TGGACGGCCT GACTCTATTC TGAGTGATGA ACGGGTTTGG 
GAGTATCCTT CTGACGTGGA TGTGTCAGCT AACAAGCCGA TTTCCATAGT TCTCACCCAA 
TTCCACTTCC TGCTGTTACT GCCTGACAAA GTGATAGCTG TGTGCACTCT GAATGGGCAG 
GTTGTCTTCC AAGATGTGTT CTTGGAGAAA TTTGGCTCTT TGAAGCGCAT GATCAAAGAT 
CCCACAGTCG GGCAGGTCTG GATCCACACA GAGAAAGTTG TATTCCGCTA CCACGTACAA 
CGGGAATCGA GAGATGTATG GAAGATGTAT ATGAACATTA ACAAGTTTGA TCTGGCCAAA 
GAGTACTGCA AAGACCGTCC TGAGTGCCTA GATATTGTGT TGGCAAGAGA GGCAGAACAC 
TCCTTTCAAA ACAAGAGGTA CTTGGATAGT GCCAAGTGTT ATGCACTGAC CCAGAATTAC 
TTTGAGGAGA TTGCCCTTAA GTTCATTGAA GCCAAACAAG AGGAGGCATT GATGGAGTTT 
CTGATTAAGA AACTGGCCAA CTTAAAGTCT TCTGAAAAGA CCCAGACAAC CTTGTTGACC 
ACCTGGTTAA CAGAGCTCTA CTTGAATCGG CTAGGTGCTT TGGAGGGTGA CGCCTCCAAA 
AGGAATCTCT ATGAGGAGAC TCGGGAGAAG TTCTGCAGCT TCCTGAGCAG CCCCAAAAAC 
AAAGAGTGTC TGTTCAATAA CCGAGTGTCC ATCTATGAGC TGCTAGCAAG CCACGGGGAC 
ACAGAACACA TGGTCTATTT TTCAGTTATT ATGCAAGACT ACGAGAGAGT GGTAGCCCAT 
CACTGCCAAC ATGATGACTA TGATGAAGCC TTAAATGTTC TGTCCAGACA CAGGGATGAA 
AAGCTCTTTT ACAAGTTCTC TCCGGTCCTG ATCCAGCACA TCCCCAAGAA GGTGGTTGAT 
GCTTGGATCT CCATGGGCAC AAAACTGGAT GCCAGGAAGC TCATACCTGC CCTCGTCAAC 
TACAGTCAAA GTGAAAGCAC CCAACAGATC AGTGAAGCTA TTCGATACAT GGAGTTCTGT 
GTGTGTGAGC TGGAAGAAAC GGAGCAAGCC ATTCACAACT ATCTGTTGTC TCTCTATGCT 
TTGTGTCGGC CAGTCTCGCT GCTGTCGTAC CTAGAACAAG CAGGAACCAA CCCTAACAGG 
ATCCACTATG ACCTGAAGTA TGCGCTTCGG CTGTGTGCAG AGCACGGACA CCACCGAGCC 
TGTGTCCATG TTTACAAAGT GATGGAGTTA TATGAGGAAG CAGTGGATCT AGCTTTGCAG 
GTGGATGTGG ATTTGGCTAA GTCCTGTGCC GACCTCCCTG AAGAGGATGA GGAACTCCGG 
AAGAAGCTCT GGTTGAAAAT TGCTCGTCAT GTAGTCCAGG AAGAGAAGGA TGTAAAGAAA 
GCCATGGCCT GCCTTTCTAG CTGCAACCTG CTGAAGATTG AAGATGTCTT GCCCTTTTTC 
CCTGACTTTG TCACCATTGA CCACTTTAAA GAGGCTATCT GCAACTCTCT GGAGGACTAC 
AACAAGCACA TTGAGGAGCT GAAAAGAGAG ATGGAGGAAG CCACGCAGAG TGCCAAGAGA 
ATTCGGGAGG ACATCCAGGA GATGAGAAAC AAATACGGAT CTGTGGAGCC TCAGGAAAAG 
TGTGCAGCCT GTGACTTTCC GCTCCTAAAT CGCCCTTTCT ACCTTTTTCT TTGTGGTCAC 
ATGTTCCATT ATGACTGCCT GTTCCAGGCA GTTTTTCCAA ACCTGCCTGC CTGGAAGCAG 
GCTAAGCTAG AAGACCTTCA GAAGAAGCTG GGAGCCACAA GCCAGCCCTC CAAAGCCCAT 
CATCGTCCCA AGGACATAGA TGCTGCTAGC TTGGGAAAAG GGCAGCAGAG TAGGGAACAG 
ATCAAGGCAG ACATTGATGA TATTGTGGCG GCTGAATGTG TGTATTGTGG GGAGTTGATG 
ATCCGGTCAA TTGACAAGCC TTTCATTGAC CCACAAAAGT ATGAAGAGGA GAGACAAAGT 
TGGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS18 ( XM_006128686.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006128748.1 Pelodiscus sinensis VPS18, CORVET/HOPS core subunit (VPS18), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPe34438
Clone ID Related Accession (Same CDS sequence)	XM_006128686.1
Accession Version	XM_006128686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	vacuolar protein sorting-associated protein 18 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005856687.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCCA TCCTGGACGA GTACGAAGAT TCGCTGTCCC GCTCGGCCGC CCAGCCGCAG 
AGCCGCGCCA GCGTGGGCAT CCCGCACTCC GGATTTGTGA ATGCTCGGCT GGAGAAGGAG 
ACACCAATAT TTAACAAGCA AAGGATTGAT TTCACTCCTC CAGAGAAAAT TAACAGTCTA 
GTGGTCTCCT CTAATCAGCT TTGTATGAGC CTTGGCAGAG ACACCCTTTT CAGGATTGAT 
CTTGGCAAGG CAGATGAACC TAATCAGGTG GAGTTGGGAC GCAAGGATGA AGCCAGAGTC 
TACAAGATGT TTTTAGATCA CACAGGATCT CACCTTGTGA TTGCACTGAA CACTAGTGAA 
TGCCTGTATT TGAACAGAAG CGCTCAGAAG GTGCGGGCGC TCTCCCGCTG GAAAGGTCAC 
TTGGTTGAAT GTGTGGGCTG GAATAAGTTC CTGGGTTCAG AGACGAACAC TGGTCCTATC 
CTGGTGGGAA CAGCCCAGGG CCAAATATAC GAGGCTGAGA TCTCTGTCAG CGAGGGAAGC 
TTCTTCAGCA CTAATCCAGA CCAGTATTTC CGTCTGGTCT ATACCTTGGA GGAGGAGGCA 
GGGCCCACCC CAGTCTGCTG TCTGGAAATA GAGAGAGTGC TCGATGGGAA ATGTTTCATC 
ATTGCCACTA CTTGTAAAAG ACTCTTCCAG TTTGCGGGCA AAGCGCTTGA GGGGACAGAG 
CAGCAGGGCT TTGGCTTTGT GTTTGGCATG CATGTAGACC ACTTGCCCAG CTTTCGAGAG 
TTCCCAGCCA GCTTTGGCTA CAGCGAAATA GCATTTTATA CCCCAAAGTT GCGCTCCTCC 
CCACGGTCCT TTGCCTGGAT GATGGGAAAT GGTGTCTTGT ATGGTACGTT GGACTATGGA 
CGGCCTGACT CTATTCTGAG TGATGAACGG GTTTGGGAGT ATCCTTCTGA CGTGGATGTG 
TCAGCTAACA AGCCGATTTC CATAGTTCTC ACCCAATTCC ACTTCCTGCT GTTACTGCCT 
GACAAAGTGA TAGCTGTGTG CACTCTGAAT GGGCAGGTTG TCTTCCAAGA TGTGTTCTTG 
GAGAAATTTG GCTCTTTGAA GCGCATGATC AAAGATCCCA CAGTCGGGCA GGTCTGGATC 
CACACAGAGA AAGTTGTATT CCGCTACCAC GTACAACGGG AATCGAGAGA TGTATGGAAG 
ATGTATATGA ACATTAACAA GTTTGATCTG GCCAAAGAGT ACTGCAAAGA CCGTCCTGAG 
TGCCTAGATA TTGTGTTGGC AAGAGAGGCA GAACACTCCT TTCAAAACAA GAGGTACTTG 
GATAGTGCCA AGTGTTATGC ACTGACCCAG AATTACTTTG AGGAGATTGC CCTTAAGTTC 
ATTGAAGCCA AACAAGAGGA GGCATTGATG GAGTTTCTGA TTAAGAAACT GGCCAACTTA 
AAGTCTTCTG AAAAGACCCA GACAACCTTG TTGACCACCT GGTTAACAGA GCTCTACTTG 
AATCGGCTAG GTGCTTTGGA GGGTGACGCC TCCAAAAGGA ATCTCTATGA GGAGACTCGG 
GAGAAGTTCT GCAGCTTCCT GAGCAGCCCC AAAAACAAAG AGTGTCTGTT CAATAACCGA 
GTGTCCATCT ATGAGCTGCT AGCAAGCCAC GGGGACACAG AACACATGGT CTATTTTTCA 
GTTATTATGC AAGACTACGA GAGAGTGGTA GCCCATCACT GCCAACATGA TGACTATGAT 
GAAGCCTTAA ATGTTCTGTC CAGACACAGG GATGAAAAGC TCTTTTACAA GTTCTCTCCG 
GTCCTGATCC AGCACATCCC CAAGAAGGTG GTTGATGCTT GGATCTCCAT GGGCACAAAA 
CTGGATGCCA GGAAGCTCAT ACCTGCCCTC GTCAACTACA GTCAAAGTGA AAGCACCCAA 
CAGATCAGTG AAGCTATTCG ATACATGGAG TTCTGTGTGT GTGAGCTGGA AGAAACGGAG 
CAAGCCATTC ACAACTATCT GTTGTCTCTC TATGCTTTGT GTCGGCCAGT CTCGCTGCTG 
TCGTACCTAG AACAAGCAGG AACCAACCCT AACAGGATCC ACTATGACCT GAAGTATGCG 
CTTCGGCTGT GTGCAGAGCA CGGACACCAC CGAGCCTGTG TCCATGTTTA CAAAGTGATG 
GAGTTATATG AGGAAGCAGT GGATCTAGCT TTGCAGGTGG ATGTGGATTT GGCTAAGTCC 
TGTGCCGACC TCCCTGAAGA GGATGAGGAA CTCCGGAAGA AGCTCTGGTT GAAAATTGCT 
CGTCATGTAG TCCAGGAAGA GAAGGATGTA AAGAAAGCCA TGGCCTGCCT TTCTAGCTGC 
AACCTGCTGA AGATTGAAGA TGTCTTGCCC TTTTTCCCTG ACTTTGTCAC CATTGACCAC 
TTTAAAGAGG CTATCTGCAA CTCTCTGGAG GACTACAACA AGCACATTGA GGAGCTGAAA 
AGAGAGATGG AGGAAGCCAC GCAGAGTGCC AAGAGAATTC GGGAGGACAT CCAGGAGATG 
AGAAACAAAT ACGGATCTGT GGAGCCTCAG GAAAAGTGTG CAGCCTGTGA CTTTCCGCTC 
CTAAATCGCC CTTTCTACCT TTTTCTTTGT GGTCACATGT TCCATTATGA CTGCCTGTTC 
CAGGCAGTTT TTCCAAACCT GCCTGCCTGG AAGCAGGCTA AGCTAGAAGA CCTTCAGAAG 
AAGCTGGGAG CCACAAGCCA GCCCTCCAAA GCCCATCATC GTCCCAAGGA CATAGATGCT 
GCTAGCTTGG GAAAAGGGCA GCAGAGTAGG GAACAGATCA AGGCAGACAT TGATGATATT 
GTGGCGGCTG AATGTGTGTA TTGTGGGGAG TTGATGATCC GGTCAATTGA CAAGCCTTTC 
ATTGACCCAC AAAAGTATGA AGAGGAGAGA CAAAGTTGGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006128748.1
CDS	109..3033
Translation	MASILDEYEDSLSRSAAQPQSRASVGIPHSGFVNARLEKETPIFNKQRIDFTPPEKINSLVVSSNQLCMSLGRDTLFRIDLGKADEPNQVELGRKDEARVYKMFLDHTGSHLVIALNTSECLYLNRSAQKVRALSRWKGHLVECVGWNKFLGSETNTGPILVGTAQGQIYEAEISVSEGSFFSTNPDQYFRLVYTLEEEAGPTPVCCLEIERVLDGKCFIIATTCKRLFQFAGKALEGTEQQGFGFVFGMHVDHLPSFREFPASFGYSEIAFYTPKLRSSPRSFAWMMGNGVLYGTLDYGRPDSILSDERVWEYPSDVDVSANKPISIVLTQFHFLLLLPDKVIAVCTLNGQVVFQDVFLEKFGSLKRMIKDPTVGQVWIHTEKVVFRYHVQRESRDVWKMYMNINKFDLAKEYCKDRPECLDIVLAREAEHSFQNKRYLDSAKCYALTQNYFEEIALKFIEAKQEEALMEFLIKKLANLKSSEKTQTTLLTTWLTELYLNRLGALEGDASKRNLYEETREKFCSFLSSPKNKECLFNNRVSIYELLASHGDTEHMVYFSVIMQDYERVVAHHCQHDDYDEALNVLSRHRDEKLFYKFSPVLIQHIPKKVVDAWISMGTKLDARKLIPALVNYSQSESTQQISEAIRYMEFCVCELEETEQAIHNYLLSLYALCRPVSLLSYLEQAGTNPNRIHYDLKYALRLCAEHGHHRACVHVYKVMELYEEAVDLALQVDVDLAKSCADLPEEDEELRKKLWLKIARHVVQEEKDVKKAMACLSSCNLLKIEDVLPFFPDFVTIDHFKEAICNSLEDYNKHIEELKREMEEATQSAKRIREDIQEMRNKYGSVEPQEKCAACDFPLLNRPFYLFLCGHMFHYDCLFQAVFPNLPAWKQAKLEDLQKKLGATSQPSKAHHRPKDIDAASLGKGQQSREQIKADIDDIVAAECVYCGELMIRSIDKPFIDPQKYEEERQSWL

Target ORF information:

RefSeq Version	XM_006128686.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis VPS18, CORVET/HOPS core subunit (VPS18), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006128686.1

ORF Insert Sequence:

ATGGCCTCCA TCCTGGACGA GTACGAAGAT TCGCTGTCCC GCTCGGCCGC CCAGCCGCAG 
AGCCGCGCCA GCGTGGGCAT CCCGCACTCC GGATTTGTGA ATGCTCGGCT GGAGAAGGAG 
ACACCAATAT TTAACAAGCA AAGGATTGAT TTCACTCCTC CAGAGAAAAT TAACAGTCTA 
GTGGTCTCCT CTAATCAGCT TTGTATGAGC CTTGGCAGAG ACACCCTTTT CAGGATTGAT 
CTTGGCAAGG CAGATGAACC TAATCAGGTG GAGTTGGGAC GCAAGGATGA AGCCAGAGTC 
TACAAGATGT TTTTAGATCA CACAGGATCT CACCTTGTGA TTGCACTGAA CACTAGTGAA 
TGCCTGTATT TGAACAGAAG CGCTCAGAAG GTGCGGGCGC TCTCCCGCTG GAAAGGTCAC 
TTGGTTGAAT GTGTGGGCTG GAATAAGTTC CTGGGTTCAG AGACGAACAC TGGTCCTATC 
CTGGTGGGAA CAGCCCAGGG CCAAATATAC GAGGCTGAGA TCTCTGTCAG CGAGGGAAGC 
TTCTTCAGCA CTAATCCAGA CCAGTATTTC CGTCTGGTCT ATACCTTGGA GGAGGAGGCA 
GGGCCCACCC CAGTCTGCTG TCTGGAAATA GAGAGAGTGC TCGATGGGAA ATGTTTCATC 
ATTGCCACTA CTTGTAAAAG ACTCTTCCAG TTTGCGGGCA AAGCGCTTGA GGGGACAGAG 
CAGCAGGGCT TTGGCTTTGT GTTTGGCATG CATGTAGACC ACTTGCCCAG CTTTCGAGAG 
TTCCCAGCCA GCTTTGGCTA CAGCGAAATA GCATTTTATA CCCCAAAGTT GCGCTCCTCC 
CCACGGTCCT TTGCCTGGAT GATGGGAAAT GGTGTCTTGT ATGGTACGTT GGACTATGGA 
CGGCCTGACT CTATTCTGAG TGATGAACGG GTTTGGGAGT ATCCTTCTGA CGTGGATGTG 
TCAGCTAACA AGCCGATTTC CATAGTTCTC ACCCAATTCC ACTTCCTGCT GTTACTGCCT 
GACAAAGTGA TAGCTGTGTG CACTCTGAAT GGGCAGGTTG TCTTCCAAGA TGTGTTCTTG 
GAGAAATTTG GCTCTTTGAA GCGCATGATC AAAGATCCCA CAGTCGGGCA GGTCTGGATC 
CACACAGAGA AAGTTGTATT CCGCTACCAC GTACAACGGG AATCGAGAGA TGTATGGAAG 
ATGTATATGA ACATTAACAA GTTTGATCTG GCCAAAGAGT ACTGCAAAGA CCGTCCTGAG 
TGCCTAGATA TTGTGTTGGC AAGAGAGGCA GAACACTCCT TTCAAAACAA GAGGTACTTG 
GATAGTGCCA AGTGTTATGC ACTGACCCAG AATTACTTTG AGGAGATTGC CCTTAAGTTC 
ATTGAAGCCA AACAAGAGGA GGCATTGATG GAGTTTCTGA TTAAGAAACT GGCCAACTTA 
AAGTCTTCTG AAAAGACCCA GACAACCTTG TTGACCACCT GGTTAACAGA GCTCTACTTG 
AATCGGCTAG GTGCTTTGGA GGGTGACGCC TCCAAAAGGA ATCTCTATGA GGAGACTCGG 
GAGAAGTTCT GCAGCTTCCT GAGCAGCCCC AAAAACAAAG AGTGTCTGTT CAATAACCGA 
GTGTCCATCT ATGAGCTGCT AGCAAGCCAC GGGGACACAG AACACATGGT CTATTTTTCA 
GTTATTATGC AAGACTACGA GAGAGTGGTA GCCCATCACT GCCAACATGA TGACTATGAT 
GAAGCCTTAA ATGTTCTGTC CAGACACAGG GATGAAAAGC TCTTTTACAA GTTCTCTCCG 
GTCCTGATCC AGCACATCCC CAAGAAGGTG GTTGATGCTT GGATCTCCAT GGGCACAAAA 
CTGGATGCCA GGAAGCTCAT ACCTGCCCTC GTCAACTACA GTCAAAGTGA AAGCACCCAA 
CAGATCAGTG AAGCTATTCG ATACATGGAG TTCTGTGTGT GTGAGCTGGA AGAAACGGAG 
CAAGCCATTC ACAACTATCT GTTGTCTCTC TATGCTTTGT GTCGGCCAGT CTCGCTGCTG 
TCGTACCTAG AACAAGCAGG AACCAACCCT AACAGGATCC ACTATGACCT GAAGTATGCG 
CTTCGGCTGT GTGCAGAGCA CGGACACCAC CGAGCCTGTG TCCATGTTTA CAAAGTGATG 
GAGTTATATG AGGAAGCAGT GGATCTAGCT TTGCAGGTGG ATGTGGATTT GGCTAAGTCC 
TGTGCCGACC TCCCTGAAGA GGATGAGGAA CTCCGGAAGA AGCTCTGGTT GAAAATTGCT 
CGTCATGTAG TCCAGGAAGA GAAGGATGTA AAGAAAGCCA TGGCCTGCCT TTCTAGCTGC 
AACCTGCTGA AGATTGAAGA TGTCTTGCCC TTTTTCCCTG ACTTTGTCAC CATTGACCAC 
TTTAAAGAGG CTATCTGCAA CTCTCTGGAG GACTACAACA AGCACATTGA GGAGCTGAAA 
AGAGAGATGG AGGAAGCCAC GCAGAGTGCC AAGAGAATTC GGGAGGACAT CCAGGAGATG 
AGAAACAAAT ACGGATCTGT GGAGCCTCAG GAAAAGTGTG CAGCCTGTGA CTTTCCGCTC 
CTAAATCGCC CTTTCTACCT TTTTCTTTGT GGTCACATGT TCCATTATGA CTGCCTGTTC 
CAGGCAGTTT TTCCAAACCT GCCTGCCTGG AAGCAGGCTA AGCTAGAAGA CCTTCAGAAG 
AAGCTGGGAG CCACAAGCCA GCCCTCCAAA GCCCATCATC GTCCCAAGGA CATAGATGCT 
GCTAGCTTGG GAAAAGGGCA GCAGAGTAGG GAACAGATCA AGGCAGACAT TGATGATATT 
GTGGCGGCTG AATGTGTGTA TTGTGGGGAG TTGATGATCC GGTCAATTGA CAAGCCTTTC 
ATTGACCCAC AAAAGTATGA AGAGGAGAGA CAAAGTTGGC TGTAG