NIT2 cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NIT2
Entrez Gene ID	102445345
Full Name	nitrilase family member 2
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006130687.3	XP_006130749.1	omega-amidase NIT2
XM_006130687.2	XP_006130749.1	omega-amidase NIT2

Pathways from BioSystems
Gene Ontology

The following NIT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPe36232	XM_006130687.3 Latest version!	Pelodiscus sinensis nitrilase family member 2 (NIT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
View Old Accession Versions >>
OPe36232	XM_006130687.2	Pelodiscus sinensis nitrilase family, member 2 (NIT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NIT2 ( XM_006130687.3 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006130749.1 Pelodiscus sinensis nitrilase family member 2 (NIT2), mRNA.

Price & Availability↑

CloneID	OPe36232
Clone ID Related Accession (Same CDS sequence)	XM_006130687.3 , XM_006130687.2
Accession Version	XM_006130687.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	omega-amidase NIT2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005857280.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 4, 2018 this sequence version replaced XM_006130687.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCGG CGCGAGGAGC CATGGCCAAC TTCCGTCTGG CGCTAATCCA ACATCATGTA 
TCTGCTGTCA AATCAGATAA TCTGAATAAA GCATGTGGAC TGGTAAAACA AGCAGCAGCA 
CAAGGAGCAA AAATTGTGGC TCTACCTGAA TGCTTTAACT CTCCCTATGG TACTAAATAT 
TTTCCTGAAT ATGCAGAGAA AATCCCTGGA GAATCTACAC AGAAGCTCTC AGAAGTTGCT 
AAGGAGTGTG GCATCTATCT CATTGGAGGA TCCATTCCGG AAAAGGATGC TGGAAAGTTG 
TATAACACAT GTACTGTGTT TGGACCTGAT GGCACTATGC TGGCTAAACA CAGAAAGATT 
CATTTATTTG ATATTAATGT TCCTGGAAAA ATCCGGTTCC AAGAGTCAGA AACTCTGAGC 
CCAGGGGACA GTTTCTCGGT GTTTGATACT CCATACTGTA AAGTGGGACT GGGGATCTGC 
TATGACATCC GATTTGCTGA GCTTGCTCAA ATCTACACAC AGAAAGGTTG CCAGCTGTTA 
GTGTATCCAG GGGCTTTTAA TATGACAACT GGACCAGCCC ACTGGGAACT GCTGCAAAGA 
GGACGAGCTG TTGATAACCA AGTCTACGTG GCTACTGCAT CTCCTGCTAG AGACGAGAGA 
GCATCTTACA TTGCCTGGGG ACATAGCACT GTAGTAAACC CCTGGGGAGA GGTCATAGCT 
AAAGCTGGCA CTGAAGAGAC CATTATCTAC ACAGATATAG ACCTGACAAA ACTAGCAGAA 
ATCCGCCAGC AAATCCCCCT TCATGCTCAA AAGCGCATAG ATCTTTACAG CATAGAGGCA 
AAGAAAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006130749.1
CDS	54..905
Translation	MLPARGAMANFRLALIQHHVSAVKSDNLNKACGLVKQAAAQGAKIVALPECFNSPYGTKYFPEYAEKIPGESTQKLSEVAKECGIYLIGGSIPEKDAGKLYNTCTVFGPDGTMLAKHRKIHLFDINVPGKIRFQESETLSPGDSFSVFDTPYCKVGLGICYDIRFAELAQIYTQKGCQLLVYPGAFNMTTGPAHWELLQRGRAVDNQVYVATASPARDERASYIAWGHSTVVNPWGEVIAKAGTEETIIYTDIDLTKLAEIRQQIPLHAQKRIDLYSIEAKKE

Target ORF information:

RefSeq Version	XM_006130687.3
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis nitrilase family member 2 (NIT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006130687.3

ORF Insert Sequence:

ATGCTGCCGG CGCGAGGAGC CATGGCCAAC TTCCGTCTGG CGCTAATCCA ACATCATGTA 
TCTGCTGTCA AATCAGATAA TCTGAATAAA GCATGTGGAC TGGTAAAACA AGCAGCAGCA 
CAAGGAGCAA AAATTGTGGC TCTACCTGAA TGCTTTAACT CTCCCTATGG TACTAAATAT 
TTTCCTGAAT ATGCAGAGAA AATCCCTGGA GAATCTACAC AGAAGCTCTC AGAAGTTGCT 
AAGGAGTGTG GCATCTATCT CATTGGAGGA TCCATTCCGG AAAAGGATGC TGGAAAGTTG 
TATAACACAT GTACTGTGTT TGGACCTGAT GGCACTATGC TGGCTAAACA CAGAAAGATT 
CATTTATTTG ATATTAATGT TCCTGGAAAA ATCCGGTTCC AAGAGTCAGA AACTCTGAGC 
CCAGGGGACA GTTTCTCGGT GTTTGATACT CCATACTGTA AAGTGGGACT GGGGATCTGC 
TATGACATCC GATTTGCTGA GCTTGCTCAA ATCTACACAC AGAAAGGTTG CCAGCTGTTA 
GTGTATCCAG GGGCTTTTAA TATGACAACT GGACCAGCCC ACTGGGAACT GCTGCAAAGA 
GGACGAGCTG TTGATAACCA AGTCTACGTG GCTACTGCAT CTCCTGCTAG AGACGAGAGA 
GCATCTTACA TTGCCTGGGG ACATAGCACT GTAGTAAACC CCTGGGGAGA GGTCATAGCT 
AAAGCTGGCA CTGAAGAGAC CATTATCTAC ACAGATATAG ACCTGACAAA ACTAGCAGAA 
ATCCGCCAGC AAATCCCCCT TCATGCTCAA AAGCGCATAG ATCTTTACAG CATAGAGGCA 
AAGAAAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIT2 ( XM_006130687.2 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006130749.1 Pelodiscus sinensis nitrilase family, member 2 (NIT2), mRNA.

Price & Availability↑

CloneID	OPe36232
Clone ID Related Accession (Same CDS sequence)	XM_006130687.3 , XM_006130687.2
Accession Version	XM_006130687.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	omega-amidase NIT2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005857280.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 3, 2015 this sequence version replaced XM_006130687.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCGG CGCGAGGAGC CATGGCCAAC TTCCGTCTGG CGCTAATCCA ACATCATGTA 
TCTGCTGTCA AATCAGATAA TCTGAATAAA GCATGTGGAC TGGTAAAACA AGCAGCAGCA 
CAAGGAGCAA AAATTGTGGC TCTACCTGAA TGCTTTAACT CTCCCTATGG TACTAAATAT 
TTTCCTGAAT ATGCAGAGAA AATCCCTGGA GAATCTACAC AGAAGCTCTC AGAAGTTGCT 
AAGGAGTGTG GCATCTATCT CATTGGAGGA TCCATTCCGG AAAAGGATGC TGGAAAGTTG 
TATAACACAT GTACTGTGTT TGGACCTGAT GGCACTATGC TGGCTAAACA CAGAAAGATT 
CATTTATTTG ATATTAATGT TCCTGGAAAA ATCCGGTTCC AAGAGTCAGA AACTCTGAGC 
CCAGGGGACA GTTTCTCGGT GTTTGATACT CCATACTGTA AAGTGGGACT GGGGATCTGC 
TATGACATCC GATTTGCTGA GCTTGCTCAA ATCTACACAC AGAAAGGTTG CCAGCTGTTA 
GTGTATCCAG GGGCTTTTAA TATGACAACT GGACCAGCCC ACTGGGAACT GCTGCAAAGA 
GGACGAGCTG TTGATAACCA AGTCTACGTG GCTACTGCAT CTCCTGCTAG AGACGAGAGA 
GCATCTTACA TTGCCTGGGG ACATAGCACT GTAGTAAACC CCTGGGGAGA GGTCATAGCT 
AAAGCTGGCA CTGAAGAGAC CATTATCTAC ACAGATATAG ACCTGACAAA ACTAGCAGAA 
ATCCGCCAGC AAATCCCCCT TCATGCTCAA AAGCGCATAG ATCTTTACAG CATAGAGGCA 
AAGAAAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006130749.1
CDS	51..902
Translation	MLPARGAMANFRLALIQHHVSAVKSDNLNKACGLVKQAAAQGAKIVALPECFNSPYGTKYFPEYAEKIPGESTQKLSEVAKECGIYLIGGSIPEKDAGKLYNTCTVFGPDGTMLAKHRKIHLFDINVPGKIRFQESETLSPGDSFSVFDTPYCKVGLGICYDIRFAELAQIYTQKGCQLLVYPGAFNMTTGPAHWELLQRGRAVDNQVYVATASPARDERASYIAWGHSTVVNPWGEVIAKAGTEETIIYTDIDLTKLAEIRQQIPLHAQKRIDLYSIEAKKE

Target ORF information:

RefSeq Version	XM_006130687.2
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis nitrilase family, member 2 (NIT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006130687.2

ORF Insert Sequence:

ATGCTGCCGG CGCGAGGAGC CATGGCCAAC TTCCGTCTGG CGCTAATCCA ACATCATGTA 
TCTGCTGTCA AATCAGATAA TCTGAATAAA GCATGTGGAC TGGTAAAACA AGCAGCAGCA 
CAAGGAGCAA AAATTGTGGC TCTACCTGAA TGCTTTAACT CTCCCTATGG TACTAAATAT 
TTTCCTGAAT ATGCAGAGAA AATCCCTGGA GAATCTACAC AGAAGCTCTC AGAAGTTGCT 
AAGGAGTGTG GCATCTATCT CATTGGAGGA TCCATTCCGG AAAAGGATGC TGGAAAGTTG 
TATAACACAT GTACTGTGTT TGGACCTGAT GGCACTATGC TGGCTAAACA CAGAAAGATT 
CATTTATTTG ATATTAATGT TCCTGGAAAA ATCCGGTTCC AAGAGTCAGA AACTCTGAGC 
CCAGGGGACA GTTTCTCGGT GTTTGATACT CCATACTGTA AAGTGGGACT GGGGATCTGC 
TATGACATCC GATTTGCTGA GCTTGCTCAA ATCTACACAC AGAAAGGTTG CCAGCTGTTA 
GTGTATCCAG GGGCTTTTAA TATGACAACT GGACCAGCCC ACTGGGAACT GCTGCAAAGA 
GGACGAGCTG TTGATAACCA AGTCTACGTG GCTACTGCAT CTCCTGCTAG AGACGAGAGA 
GCATCTTACA TTGCCTGGGG ACATAGCACT GTAGTAAACC CCTGGGGAGA GGTCATAGCT 
AAAGCTGGCA CTGAAGAGAC CATTATCTAC ACAGATATAG ACCTGACAAA ACTAGCAGAA 
ATCCGCCAGC AAATCCCCCT TCATGCTCAA AAGCGCATAG ATCTTTACAG CATAGAGGCA 
AAGAAAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.