NSF cDNA ORF clone, Pelecanus crispus(Dalmatian pelican)

Gene
Clones
gRNAs

Summary

Gene Symbol	NSF
Entrez Gene ID	104033909
Full Name	N-ethylmaleimide sensitive factor, vesicle fusing ATPase
Gene Type	protein-coding
Organism	Pelecanus crispus(Dalmatian pelican)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009486708.1	XP_009484983.1	vesicle-fusing ATPase

Pathways from BioSystems
Gene Ontology

The following NSF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPe122039	XM_009486708.1 Latest version!	Pelecanus crispus N-ethylmaleimide-sensitive factor (NSF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSF ( XM_009486708.1 ) cDNA ORF clone, Pelecanus crispus(Dalmatian pelican) -> XP_009484983.1 Pelecanus crispus N-ethylmaleimide-sensitive factor (NSF), mRNA.

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CloneID	OPe122039
Clone ID Related Accession (Same CDS sequence)	XM_009486708.1
Accession Version	XM_009486708.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-05
Organism	Pelecanus crispus(Dalmatian pelican)
Product	vesicle-fusing ATPase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009097419.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelecanus crispus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGCCACATG TTGTTGTGAA GACTTCTTCC AACCACAAAT ACGTTTTCAC GCTGAGAACC 
CATTCCTCAG TTGTTCCTGG CAGCATCGCG TTCAGCTTGC CCCAGAGAAA ATGGGCTGGA 
CTTTCCATTG GACAAGAGAT AGATGTTTCC TTATACACTT TCGACAAGGC TAAGCAGTGT 
ATTGGCACAA TGACAGTTGA GATAGATTTT CTGCAGAAGA AGAACATTGA CTCCAACCCC 
TACGACACAG ACAAGATGGC TGCTGAATTC ATCCAGCAAT TCAATAGCCA AGCCTTCTCA 
GTAGGCCAGC AGCTTGTGTT CAGCTTCAAT GACAAACTTT TTGGCCTGTT GGTAAAGGAC 
ATGGAAGCTA TGGACCCCAG CATTCTCAAA GGAGAGTCTG GCGCAAGCAA AAAGCAGAAG 
ATTGAGGTTG GTTTGGTTCT TGGAAACAGT CAAGTTGCCT TTGAAAAAGC TGAGAACTCC 
CCTCTCAATC TGATTGGTAA ATCAAAAACC AAGGAGAACC GCCAGTCGAT CATCAACCCA 
GACTGGAATT TTGAGAAAAT GGGCATTGGG GGCCTTGACA AAGAATTCTC GGATATTTTC 
CGACGAGCTT TTGCTTCTCG AGTTTTTCCA CCTGAAATTG TGGAGCAGAT GGGCTGCAAG 
CATGTGAAAG GCATCCTCTT GTATGGACCT CCAGGCTGTG GCAAAACACT TATGGCGAGA 
CAGATTGGCA AGATGCTGAA TGCCAGAGAA CCAAAGGTGG TCAATGGTCC AGAAATCCTC 
AATAAATATG TGGGCGAATC TGAGGCCAAC ATCCGCAAGC TGTTTGCTGA TGCAGAAGAA 
GAACAAAGAA GGCTTGGAGC AAACAGCGGT GTGCATATCA TCATTTTTGA TGAGATTGAT 
GCAATCTGCA AGCAGAGAGG AAGCATGGCG GGTAGCACTG GAGTCCATGA TACTGTTGTA 
AACCAGTTGC TGTCTAAAAT TGATGGAGTA GAGCAGCTCA ATAACATCCT CGTGATTGGA 
ATGACCAACA GACCTGACCT GATTGATGAG GCTCTGCTGA GACCAGGGAG ACTGGAGGTG 
AAAATGGAGA TTGGTTTGCC GGATGAGAAG GGTCGATTTC AGATTCTTCA TATCCACACA 
GTACGGATGC GGGAACACCA GCTGCTGGCT GAAGATGTGG ACATTGCAGA ACTGGCAGTC 
GAGACTAAAA ACTTCAGCGG TGCTGAATTA GAAGGTTTAG TTCGAGCTGC TCAGTCTACT 
GCTATGAACA GACACATAAA GGCCAGCAAC AAAGTGGAAG TGGATATGGA GAAGGCAGAG 
AGCTTGCGAG TGACAAGAGG AGACTTCTTT GCATCTTTGG AGAATGATAT CAAACCAGCG 
TTTGGAACCA ACCAGGAAGA CTATGCAAGC TACATCATGA ATGGTATTAT TAAGTGGGGT 
GATCCAGTAA CAAGGGTATT GGATGATGGG GAGCTGCTTG TTCAACAAAC TAAGAACAGC 
GACCGCACTC CTTTGGTTAG CGTTCTTCTA GAAGGTCCTC CCCACAGTGG GAAGACTGCT 
TTAGCAGCAA AAATAGCAGA AGAATCCAAC TTCCCCTTTA TCAAGATCTG TTCTCCTGAT 
AAGATGATCG GATTTTCTGA AACGGCCAAG TGCCAAGCTA TGAAGAAGGT ACTTAAGCGT 
CCTACTCTTT TGCTTGATTA CAGTCCAATT ATGTTCTCTA GTGGACAGAA AAGTGAATTA 
CCCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009484983.1
CDS	1..1749
Translation	MPHVVVKTSSNHKYVFTLRTHSSVVPGSIAFSLPQRKWAGLSIGQEIDVSLYTFDKAKQCIGTMTVEIDFLQKKNIDSNPYDTDKMAAEFIQQFNSQAFSVGQQLVFSFNDKLFGLLVKDMEAMDPSILKGESGASKKQKIEVGLVLGNSQVAFEKAENSPLNLIGKSKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGVHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRFQILHIHTVRMREHQLLAEDVDIAELAVETKNFSGAELEGLVRAAQSTAMNRHIKASNKVEVDMEKAESLRVTRGDFFASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKVLKRPTLLLDYSPIMFSSGQKSELPL

Target ORF information:

RefSeq Version	XM_009486708.1
Organism	Pelecanus crispus(Dalmatian pelican)
Definition	Pelecanus crispus N-ethylmaleimide-sensitive factor (NSF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009486708.1

ORF Insert Sequence:

ATGCCACATG TTGTTGTGAA GACTTCTTCC AACCACAAAT ACGTTTTCAC GCTGAGAACC 
CATTCCTCAG TTGTTCCTGG CAGCATCGCG TTCAGCTTGC CCCAGAGAAA ATGGGCTGGA 
CTTTCCATTG GACAAGAGAT AGATGTTTCC TTATACACTT TCGACAAGGC TAAGCAGTGT 
ATTGGCACAA TGACAGTTGA GATAGATTTT CTGCAGAAGA AGAACATTGA CTCCAACCCC 
TACGACACAG ACAAGATGGC TGCTGAATTC ATCCAGCAAT TCAATAGCCA AGCCTTCTCA 
GTAGGCCAGC AGCTTGTGTT CAGCTTCAAT GACAAACTTT TTGGCCTGTT GGTAAAGGAC 
ATGGAAGCTA TGGACCCCAG CATTCTCAAA GGAGAGTCTG GCGCAAGCAA AAAGCAGAAG 
ATTGAGGTTG GTTTGGTTCT TGGAAACAGT CAAGTTGCCT TTGAAAAAGC TGAGAACTCC 
CCTCTCAATC TGATTGGTAA ATCAAAAACC AAGGAGAACC GCCAGTCGAT CATCAACCCA 
GACTGGAATT TTGAGAAAAT GGGCATTGGG GGCCTTGACA AAGAATTCTC GGATATTTTC 
CGACGAGCTT TTGCTTCTCG AGTTTTTCCA CCTGAAATTG TGGAGCAGAT GGGCTGCAAG 
CATGTGAAAG GCATCCTCTT GTATGGACCT CCAGGCTGTG GCAAAACACT TATGGCGAGA 
CAGATTGGCA AGATGCTGAA TGCCAGAGAA CCAAAGGTGG TCAATGGTCC AGAAATCCTC 
AATAAATATG TGGGCGAATC TGAGGCCAAC ATCCGCAAGC TGTTTGCTGA TGCAGAAGAA 
GAACAAAGAA GGCTTGGAGC AAACAGCGGT GTGCATATCA TCATTTTTGA TGAGATTGAT 
GCAATCTGCA AGCAGAGAGG AAGCATGGCG GGTAGCACTG GAGTCCATGA TACTGTTGTA 
AACCAGTTGC TGTCTAAAAT TGATGGAGTA GAGCAGCTCA ATAACATCCT CGTGATTGGA 
ATGACCAACA GACCTGACCT GATTGATGAG GCTCTGCTGA GACCAGGGAG ACTGGAGGTG 
AAAATGGAGA TTGGTTTGCC GGATGAGAAG GGTCGATTTC AGATTCTTCA TATCCACACA 
GTACGGATGC GGGAACACCA GCTGCTGGCT GAAGATGTGG ACATTGCAGA ACTGGCAGTC 
GAGACTAAAA ACTTCAGCGG TGCTGAATTA GAAGGTTTAG TTCGAGCTGC TCAGTCTACT 
GCTATGAACA GACACATAAA GGCCAGCAAC AAAGTGGAAG TGGATATGGA GAAGGCAGAG 
AGCTTGCGAG TGACAAGAGG AGACTTCTTT GCATCTTTGG AGAATGATAT CAAACCAGCG 
TTTGGAACCA ACCAGGAAGA CTATGCAAGC TACATCATGA ATGGTATTAT TAAGTGGGGT 
GATCCAGTAA CAAGGGTATT GGATGATGGG GAGCTGCTTG TTCAACAAAC TAAGAACAGC 
GACCGCACTC CTTTGGTTAG CGTTCTTCTA GAAGGTCCTC CCCACAGTGG GAAGACTGCT 
TTAGCAGCAA AAATAGCAGA AGAATCCAAC TTCCCCTTTA TCAAGATCTG TTCTCCTGAT 
AAGATGATCG GATTTTCTGA AACGGCCAAG TGCCAAGCTA TGAAGAAGGT ACTTAAGCGT 
CCTACTCTTT TGCTTGATTA CAGTCCAATT ATGTTCTCTA GTGGACAGAA AAGTGAATTA 
CCCTTGTGA