NEFM cDNA ORF clone, Parus major(Great Tit)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEFM
Entrez Gene ID	107213992
Full Name	neurofilament medium
Gene Type	protein-coding
Organism	Parus major(Great Tit)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015648974.2	XP_015504460.1	neurofilament medium polypeptide

Pathways from BioSystems
Gene Ontology

The following NEFM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEFM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa171154	XM_015648974.2 Latest version!	Parus major neurofilament, medium polypeptide (NEFM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEFM ( XM_015648974.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015504460.1 Parus major neurofilament, medium polypeptide (NEFM), mRNA.

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CloneID	OPa171154
Clone ID Related Accession (Same CDS sequence)	XM_015648974.2
Accession Version	XM_015648974.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	neurofilament medium polypeptide
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031790.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015648974.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACA CCATGGAGCC CCTGGGCAAC CCCTCGTACC GCCGGGTGAC CGAGACCCGG 
GCCACCTACA GCCGCGCCAG CGCGTCCCCG TCCAGCGGCT TCCGCTCGCA GTCCTGGTCG 
AGGGGCTCGG GCAGCACCGT GTCCTCCTCC TACAAGCGCC CCAACCTGGG CGGCCCGCGG 
GCCGCCTACG GCTCCACGGT GCTGAGCTCG GCCGAGAGCC TGGACGTGAG CCAGTCCTCG 
CTGCTCAACG GCGCGGCCGA GCTCAAGCTG AGCCGCTCCA ACGAGAAGGA GCAGCTGCAG 
GGGCTCAACG ACCGCTTCGC CGGCTATATC GAGAAGGTGC ATTACCTGGA GCAGCAGAAC 
AAGGAGATCG AGGCGGAGCT GGCGGCGCTG CGGCAGAAAC ACGCCGGGCG GGCGCAGCTC 
AGCGACGCCT ACGAGCAGGA GCTGCGGGAG CTGCGCGGGG CGCTGGAGCA GGTGAGCCAC 
GAGAAGGCGC AGATCCAGCT GGACTCGGAG CACATCGAGG AGGACATCCA GCGCCTGCGG 
GAGCGCTTCG AGGATGAGGC GCGGCTCCGC GACGAGACGG AGGCGACGAT CCGCGCCCTG 
CGCAAGGAGA TGGAGGAGGC CTCGCTGATG CGAGCCGAAC TGGACAAGAA GGTGCAATCC 
CTGCAGGACG AGGTGGCCTT CCTGAGGGGC AACCACGAGG AGGAGGTGGC CGAGCTGCTG 
GCGCAGCTGC AGGCGTCCCA CGCCACTGTG GAGAGGAAGG ACTACCTGAA GACAGACCTG 
ACGACGGCGC TGAAGGAGAT CCGTGCCCAG CTCGAGTGCC AGTCCGACCA CAACATGCAC 
CAGGCCGAGG AGTGGTTCAA GTGCCGCTAC GCCAAGCTCA CCGAGGCGGC CGAGCAGAAC 
AAGGAGGCGA TCCGCTCCGC CAAGGAGGAG ATCGCCGAGT ACCGCCGCCA GCTCCAGTCC 
AAGAGCATCG AGCTCGAGTC CGTGCGCGGC ACCAAGGAGT CGCTGGAGAG GCAGCTCAGC 
GACATCGAGG AGCGCCACAA TAACGACCTC AGCACCTATC AGGACACGAT TCACCAGCTG 
GAAAACGAGC TCAGAGGAAC GAAGTGGGAA ATGGCTCGTC ACTTGAGGGA GTACCAGGAC 
CTCCTCAATG TCAAGATGGC CCTGGACATC GAAATTGCTG CATACAGGAA GCTGCTGGAG 
GGTGAAGAGA CAAGATTCAG TGCCTTCTCT GGAAGCATTA CTGGTCCCAT ATTCACACAT 
AGACAACCAT CTGTCACAAT AGCGTCCACC AAAATCCAGA AAACAAAAAT TGAACCACCA 
AAGCTGAAGG TCCAGCACAA GTTTGTAGAA GAAATCATTG AAGAGACGAA GGTGGAGGAT 
GAGAAGTCTG AAATGGAAGA TGCCCTGGCA GCCATCGCAG AAGAAATGGC AGCCAAGGCA 
CAGCAAGAGG AGCAGGAGGA AGAAAAAGCA GAAGAAGCTG CAGAGGAAGA AGTTTCTGAG 
AAGGCTCCTG CGGAGCAAGA AGCTGCACCT GAGGAGGAAG AGGAGGAGGA AGAAGCTGCA 
AAATCTGATG CTGCGGAAGA AGGTGGCTCT GAAAAAGAAG AAATCGAAGA AAAGGAAGAA 
GGGGAGGAAG CTGAGGAAGA GGAGGAAAAA CCTGAGGCCA AGGGCAAAGC TGAAGAGGTA 
CCAGCAAAAG CAGAGAAGGT CAAAACACCT CCCATAAAGT CCCCCCCGAA ATCCCCAGTA 
ACAGAGCCGG CCAAGGCCAC CCTGAAGGAA AAAGCTGTAG AAGCAGCCAA GGAGACGAAG 
GTGGAGAAAG CAGAGAAGGT GGTCAAGGAG GAGGAGAAAG CGGCGTCTCC AGAGAAACCT 
GCCACACCAA AGGTGACCTC CCCGGAGAAG GCTGTGACCC CGGAGAAGGC TGTGACCCCG 
GAGAAGGCTG TGACCCCGGA GAAGCTGGCA CCACCTGAGA AGCCGCGCTC CCCCGAGAAG 
CTGGCAAGCC CAGAGAAGCC GCGCACTCCT GAGAAGCCCG TGACTCCCGA GAAATCTGTG 
ACTCCCGAGA AACCCCGGTC CCCGGAGAAG CCTCCCTCCC CTGGCAAGGA TGCCAAGGCT 
GTGGTGGAGG AGACCATCAC TGTCACCAAG GTAATGAAAG TGAGCGGCGA GGCCGAGAAG 
GAGTCCAGGA AAGAGGACAT TGCGGTCAAC GGGGAGGTGG AGGAGAAGAA GGAGGAGGAA 
TCTAAAGAGA AGGAGGAGGA GGACAAGGGA GTGGTCACTA ATGGCCTCGA TGTGAGCCCC 
GTGGATGACA AGGCTCAGAA AATTGTAGTA ACCAAAAAAG TGGAGAAAAT CACAGGGGAG 
GGTGGGGACA GTGGCACCAC GTTCATCACA AAATCGGTGA CCGTCACTCA GAAGGTGGAG 
GAGCACGAAG AAAGCTTTGA GGAGAAGCTG GTGTCCACCA AGAAAGTGGA GAAAGTCACT 
TCACATGCTG TAGTAAAGGA GATTAAAGAG AGCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015504460.1
CDS	71..2569
Translation	MSYTMEPLGNPSYRRVTETRATYSRASASPSSGFRSQSWSRGSGSTVSSSYKRPNLGGPRAAYGSTVLSSAESLDVSQSSLLNGAAELKLSRSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAELAALRQKHAGRAQLSDAYEQELRELRGALEQVSHEKAQIQLDSEHIEEDIQRLRERFEDEARLRDETEATIRALRKEMEEASLMRAELDKKVQSLQDEVAFLRGNHEEEVAELLAQLQASHATVERKDYLKTDLTTALKEIRAQLECQSDHNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNNDLSTYQDTIHQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSAFSGSITGPIFTHRQPSVTIASTKIQKTKIEPPKLKVQHKFVEEIIEETKVEDEKSEMEDALAAIAEEMAAKAQQEEQEEEKAEEAAEEEVSEKAPAEQEAAPEEEEEEEEAAKSDAAEEGGSEKEEIEEKEEGEEAEEEEEKPEAKGKAEEVPAKAEKVKTPPIKSPPKSPVTEPAKATLKEKAVEAAKETKVEKAEKVVKEEEKAASPEKPATPKVTSPEKAVTPEKAVTPEKAVTPEKLAPPEKPRSPEKLASPEKPRTPEKPVTPEKSVTPEKPRSPEKPPSPGKDAKAVVEETITVTKVMKVSGEAEKESRKEDIAVNGEVEEKKEEESKEKEEEDKGVVTNGLDVSPVDDKAQKIVVTKKVEKITGEGGDSGTTFITKSVTVTQKVEEHEESFEEKLVSTKKVEKVTSHAVVKEIKESE

Target ORF information:

RefSeq Version	XM_015648974.2
Organism	Parus major(Great Tit)
Definition	Parus major neurofilament, medium polypeptide (NEFM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015648974.2

ORF Insert Sequence:

ATGAGCTACA CCATGGAGCC CCTGGGCAAC CCCTCGTACC GCCGGGTGAC CGAGACCCGG 
GCCACCTACA GCCGCGCCAG CGCGTCCCCG TCCAGCGGCT TCCGCTCGCA GTCCTGGTCG 
AGGGGCTCGG GCAGCACCGT GTCCTCCTCC TACAAGCGCC CCAACCTGGG CGGCCCGCGG 
GCCGCCTACG GCTCCACGGT GCTGAGCTCG GCCGAGAGCC TGGACGTGAG CCAGTCCTCG 
CTGCTCAACG GCGCGGCCGA GCTCAAGCTG AGCCGCTCCA ACGAGAAGGA GCAGCTGCAG 
GGGCTCAACG ACCGCTTCGC CGGCTATATC GAGAAGGTGC ATTACCTGGA GCAGCAGAAC 
AAGGAGATCG AGGCGGAGCT GGCGGCGCTG CGGCAGAAAC ACGCCGGGCG GGCGCAGCTC 
AGCGACGCCT ACGAGCAGGA GCTGCGGGAG CTGCGCGGGG CGCTGGAGCA GGTGAGCCAC 
GAGAAGGCGC AGATCCAGCT GGACTCGGAG CACATCGAGG AGGACATCCA GCGCCTGCGG 
GAGCGCTTCG AGGATGAGGC GCGGCTCCGC GACGAGACGG AGGCGACGAT CCGCGCCCTG 
CGCAAGGAGA TGGAGGAGGC CTCGCTGATG CGAGCCGAAC TGGACAAGAA GGTGCAATCC 
CTGCAGGACG AGGTGGCCTT CCTGAGGGGC AACCACGAGG AGGAGGTGGC CGAGCTGCTG 
GCGCAGCTGC AGGCGTCCCA CGCCACTGTG GAGAGGAAGG ACTACCTGAA GACAGACCTG 
ACGACGGCGC TGAAGGAGAT CCGTGCCCAG CTCGAGTGCC AGTCCGACCA CAACATGCAC 
CAGGCCGAGG AGTGGTTCAA GTGCCGCTAC GCCAAGCTCA CCGAGGCGGC CGAGCAGAAC 
AAGGAGGCGA TCCGCTCCGC CAAGGAGGAG ATCGCCGAGT ACCGCCGCCA GCTCCAGTCC 
AAGAGCATCG AGCTCGAGTC CGTGCGCGGC ACCAAGGAGT CGCTGGAGAG GCAGCTCAGC 
GACATCGAGG AGCGCCACAA TAACGACCTC AGCACCTATC AGGACACGAT TCACCAGCTG 
GAAAACGAGC TCAGAGGAAC GAAGTGGGAA ATGGCTCGTC ACTTGAGGGA GTACCAGGAC 
CTCCTCAATG TCAAGATGGC CCTGGACATC GAAATTGCTG CATACAGGAA GCTGCTGGAG 
GGTGAAGAGA CAAGATTCAG TGCCTTCTCT GGAAGCATTA CTGGTCCCAT ATTCACACAT 
AGACAACCAT CTGTCACAAT AGCGTCCACC AAAATCCAGA AAACAAAAAT TGAACCACCA 
AAGCTGAAGG TCCAGCACAA GTTTGTAGAA GAAATCATTG AAGAGACGAA GGTGGAGGAT 
GAGAAGTCTG AAATGGAAGA TGCCCTGGCA GCCATCGCAG AAGAAATGGC AGCCAAGGCA 
CAGCAAGAGG AGCAGGAGGA AGAAAAAGCA GAAGAAGCTG CAGAGGAAGA AGTTTCTGAG 
AAGGCTCCTG CGGAGCAAGA AGCTGCACCT GAGGAGGAAG AGGAGGAGGA AGAAGCTGCA 
AAATCTGATG CTGCGGAAGA AGGTGGCTCT GAAAAAGAAG AAATCGAAGA AAAGGAAGAA 
GGGGAGGAAG CTGAGGAAGA GGAGGAAAAA CCTGAGGCCA AGGGCAAAGC TGAAGAGGTA 
CCAGCAAAAG CAGAGAAGGT CAAAACACCT CCCATAAAGT CCCCCCCGAA ATCCCCAGTA 
ACAGAGCCGG CCAAGGCCAC CCTGAAGGAA AAAGCTGTAG AAGCAGCCAA GGAGACGAAG 
GTGGAGAAAG CAGAGAAGGT GGTCAAGGAG GAGGAGAAAG CGGCGTCTCC AGAGAAACCT 
GCCACACCAA AGGTGACCTC CCCGGAGAAG GCTGTGACCC CGGAGAAGGC TGTGACCCCG 
GAGAAGGCTG TGACCCCGGA GAAGCTGGCA CCACCTGAGA AGCCGCGCTC CCCCGAGAAG 
CTGGCAAGCC CAGAGAAGCC GCGCACTCCT GAGAAGCCCG TGACTCCCGA GAAATCTGTG 
ACTCCCGAGA AACCCCGGTC CCCGGAGAAG CCTCCCTCCC CTGGCAAGGA TGCCAAGGCT 
GTGGTGGAGG AGACCATCAC TGTCACCAAG GTAATGAAAG TGAGCGGCGA GGCCGAGAAG 
GAGTCCAGGA AAGAGGACAT TGCGGTCAAC GGGGAGGTGG AGGAGAAGAA GGAGGAGGAA 
TCTAAAGAGA AGGAGGAGGA GGACAAGGGA GTGGTCACTA ATGGCCTCGA TGTGAGCCCC 
GTGGATGACA AGGCTCAGAA AATTGTAGTA ACCAAAAAAG TGGAGAAAAT CACAGGGGAG 
GGTGGGGACA GTGGCACCAC GTTCATCACA AAATCGGTGA CCGTCACTCA GAAGGTGGAG 
GAGCACGAAG AAAGCTTTGA GGAGAAGCTG GTGTCCACCA AGAAAGTGGA GAAAGTCACT 
TCACATGCTG TAGTAAAGGA GATTAAAGAG AGCGAATAA