TRMT2B cDNA ORF clone, Parus major(Great Tit)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRMT2B
Entrez Gene ID	107204017
Full Name	tRNA methyltransferase 2 homolog B
Gene Type	protein-coding
Organism	Parus major(Great Tit)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015626770.2	XP_015482256.1	tRNA (uracil(54)-C(5))-methyltransferase homolog

Pathways from BioSystems
Gene Ontology

The following TRMT2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRMT2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa178298	XM_015626770.2 Latest version!	Parus major tRNA methyltransferase 2 homolog B (TRMT2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRMT2B ( XM_015626770.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015482256.1 Parus major tRNA methyltransferase 2 homolog B (TRMT2B), mRNA.

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CloneID	OPa178298
Clone ID Related Accession (Same CDS sequence)	XM_015626770.2
Accession Version	XM_015626770.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	tRNA (uracil(54)-C(5))-methyltransferase homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015626770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGT CCTGTGTGCT GAGGCCACCG CGCTGCCACC TCCGCCCTCC GCCGCTCTCC 
CCGCTCAGCC TGCCCGCTCC GCACCGCGGC CTGCTGACAG CCACAGCAGC AATGTGCCAA 
GGGAAGAAAA AGGCCAGCGT GAGCTGCAGC CTGCCAGAGC CTTCCTGGGA GGAGAGGTTA 
GCAAATGCAG TGACACCACT GTGGAGACTT CCCTACCAAG AGCAGCTGCA GGTCAAGTAC 
GAAAGCCAGA GGAAGATTTT GCAGACATTG GCATCTCGTC TTGAGGAGCT GGGCATAGAT 
GCACAGAAAC CTGGTGGGCT CTGCTGTCCT CTGCAGCCTG TAGTCCCTTC GCCCATCATT 
AATGGCTACC GAAACAAATC AACTTTCTCT GTGAACCGAG GACCAGATGG GAACCCCAAA 
ACTGTTGGGT TGTATGTAGG AACTGGCAGA GGAAGAAATA TTGTCTGTGT GAAAGCAAAT 
CATGTGGAGA ACATACCTTC AAAACACAAA CAAGTAGCCC AGTGCTATGA GGAGTTCATC 
TCTCGCTCCC CCCTGGACTC CTGCATCCTC TTCCATGAAG GGGGACACTG GCGAGAGCTC 
GTGGTTCGTA CCACCAGCTG TGGCCACACC ATGGCTATCA TCACCTTCCA CCCCCAGCAG 
CTGGGCCAGG AGGCACTGGC TACTCAGAAA GCATTGCTGA AGGAGTTCTT CACATGTGGG 
CCTGGAGCAG TCTGTGCCTT GACTTCACTT TATTTCCAAG AGAGCACCAT GACACGCTGC 
TCCCACGAGC AGTCCCCCTA CCAGCTCCTC CACGGAGCAC CGCACATCTT CGAAGAACTG 
CTCGGCCTGA AGTTTCGCAT CTCTCCAGAC GCCTTTTTCC AAGTCAACAC AGCTGGAGCA 
GAAGTTCTGT ACCAGGCAGT CGGGGAGCTC TGCCAGGCTA CAGGGGACAC TGTTCTCCTT 
GACATCTGCT GTGGAACAGG CACAATTGGT CTCTCTCTGG CTCACCAGGT GTCCAAGGTT 
ATTGGTGTTG AGGTGGTGGA GAAGGCAATA GAGGATGCTC AGTGGAATGC AGCATTCAAT 
GGGATCTCAA ACTGTGAGTT TCACAGTGGA AAGGCAGAGG CTGTGTTACC ACAGCTCTTG 
GCATCGTGGG AGGATGCCCG ACCGCTTGTT GCTGTGGTGA ACCCTTCTCG GGCTGGCCTA 
CATTACAGGG TTGTACGAGC CATCCGGAAC TGCAGGTCCA TCCGCAGGCT GCTCTACATC 
TCCTGCAAGC CAGAGGGTGA AGCCATGAGG AACTTCCTTG AGCTGTGCTG CCCACCTGAC 
CCACAGAAGA AGCTGGCAGG GGAGCCATTT GCCCCTGTGT TGGCTATTCC TTTTGATATG 
TTTCCTCACA CTGTTCACTG TGAGCTGGTG CTGCTCTTTA CCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015482256.1
CDS	747..2174
Translation	MALSCVLRPPRCHLRPPPLSPLSLPAPHRGLLTATAAMCQGKKKASVSCSLPEPSWEERLANAVTPLWRLPYQEQLQVKYESQRKILQTLASRLEELGIDAQKPGGLCCPLQPVVPSPIINGYRNKSTFSVNRGPDGNPKTVGLYVGTGRGRNIVCVKANHVENIPSKHKQVAQCYEEFISRSPLDSCILFHEGGHWRELVVRTTSCGHTMAIITFHPQQLGQEALATQKALLKEFFTCGPGAVCALTSLYFQESTMTRCSHEQSPYQLLHGAPHIFEELLGLKFRISPDAFFQVNTAGAEVLYQAVGELCQATGDTVLLDICCGTGTIGLSLAHQVSKVIGVEVVEKAIEDAQWNAAFNGISNCEFHSGKAEAVLPQLLASWEDARPLVAVVNPSRAGLHYRVVRAIRNCRSIRRLLYISCKPEGEAMRNFLELCCPPDPQKKLAGEPFAPVLAIPFDMFPHTVHCELVLLFTR

Target ORF information:

RefSeq Version	XM_015626770.2
Organism	Parus major(Great Tit)
Definition	Parus major tRNA methyltransferase 2 homolog B (TRMT2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015626770.2

ORF Insert Sequence:

ATGGCTCTGT CCTGTGTGCT GAGGCCACCG CGCTGCCACC TCCGCCCTCC GCCGCTCTCC 
CCGCTCAGCC TGCCCGCTCC GCACCGCGGC CTGCTGACAG CCACAGCAGC AATGTGCCAA 
GGGAAGAAAA AGGCCAGCGT GAGCTGCAGC CTGCCAGAGC CTTCCTGGGA GGAGAGGTTA 
GCAAATGCAG TGACACCACT GTGGAGACTT CCCTACCAAG AGCAGCTGCA GGTCAAGTAC 
GAAAGCCAGA GGAAGATTTT GCAGACATTG GCATCTCGTC TTGAGGAGCT GGGCATAGAT 
GCACAGAAAC CTGGTGGGCT CTGCTGTCCT CTGCAGCCTG TAGTCCCTTC GCCCATCATT 
AATGGCTACC GAAACAAATC AACTTTCTCT GTGAACCGAG GACCAGATGG GAACCCCAAA 
ACTGTTGGGT TGTATGTAGG AACTGGCAGA GGAAGAAATA TTGTCTGTGT GAAAGCAAAT 
CATGTGGAGA ACATACCTTC AAAACACAAA CAAGTAGCCC AGTGCTATGA GGAGTTCATC 
TCTCGCTCCC CCCTGGACTC CTGCATCCTC TTCCATGAAG GGGGACACTG GCGAGAGCTC 
GTGGTTCGTA CCACCAGCTG TGGCCACACC ATGGCTATCA TCACCTTCCA CCCCCAGCAG 
CTGGGCCAGG AGGCACTGGC TACTCAGAAA GCATTGCTGA AGGAGTTCTT CACATGTGGG 
CCTGGAGCAG TCTGTGCCTT GACTTCACTT TATTTCCAAG AGAGCACCAT GACACGCTGC 
TCCCACGAGC AGTCCCCCTA CCAGCTCCTC CACGGAGCAC CGCACATCTT CGAAGAACTG 
CTCGGCCTGA AGTTTCGCAT CTCTCCAGAC GCCTTTTTCC AAGTCAACAC AGCTGGAGCA 
GAAGTTCTGT ACCAGGCAGT CGGGGAGCTC TGCCAGGCTA CAGGGGACAC TGTTCTCCTT 
GACATCTGCT GTGGAACAGG CACAATTGGT CTCTCTCTGG CTCACCAGGT GTCCAAGGTT 
ATTGGTGTTG AGGTGGTGGA GAAGGCAATA GAGGATGCTC AGTGGAATGC AGCATTCAAT 
GGGATCTCAA ACTGTGAGTT TCACAGTGGA AAGGCAGAGG CTGTGTTACC ACAGCTCTTG 
GCATCGTGGG AGGATGCCCG ACCGCTTGTT GCTGTGGTGA ACCCTTCTCG GGCTGGCCTA 
CATTACAGGG TTGTACGAGC CATCCGGAAC TGCAGGTCCA TCCGCAGGCT GCTCTACATC 
TCCTGCAAGC CAGAGGGTGA AGCCATGAGG AACTTCCTTG AGCTGTGCTG CCCACCTGAC 
CCACAGAAGA AGCTGGCAGG GGAGCCATTT GCCCCTGTGT TGGCTATTCC TTTTGATATG 
TTTCCTCACA CTGTTCACTG TGAGCTGGTG CTGCTCTTTA CCCGCTGA