LOC109648170 cDNA ORF clone, Paralichthys olivaceus(Japanese flounder)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109648170
Entrez Gene ID	109648170
Full Name	serum response factor-like
Gene Type	protein-coding
Organism	Paralichthys olivaceus(Japanese flounder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020113965.1	XP_019969524.1	serum response factor-like

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Identification and expression of SRF targeted by miR-133a during early development of Paralichthys olivaceus.
Su Y, Fu Y, Zhang H, Shi Z, Zhang J, Gao L
Fish physiology and biochemistry41(5)1093-104(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC109648170 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109648170 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa259622	XM_020113965.1 Latest version!	Paralichthys olivaceus serum response factor-like (LOC109648170), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109648170 ( XM_020113965.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019969524.1 Paralichthys olivaceus serum response factor-like (LOC109648170), mRNA.

Price & Availability↑

CloneID	OPa259622
Clone ID Related Accession (Same CDS sequence)	XM_020113965.1
Accession Version	XM_020113965.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	serum response factor-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017866079.1) and transcript sequence (KJ862537.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1231 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGGCACCA GAACCGGACC CGGACTCACG GGGAGCACGA CTGGGACCAA CTCAGCCTCT 
GACACCGGGG GGGGGCGGTT CGAGGAGAGA GAGATCAGCA CCGAGGTGAT TTACAGCGGC 
TCCGACCAGG ACTCCGAGTC CGGGGACGAG GAGGACACAC CGGGTTCAGG AGGGGACAGG 
AGAGGGGTGA AGAGGGAGAG GAGCGAGAGG GAGGTCGGCC GTCAGGCAGT GCCCACCTCC 
GGCGGACAGA GCGGGGGTTA CGTCGGGGTC AGTCCCGGGG TGCCAGGGGC CAAACCCGGC 
AAGAAAACCA GAGGAAGAGT CAAGATCAAG ATGGAGTTCA TCGACAACAA ACTGAGGAGG 
TACACGACCT TCAGCAAGAG GAAGACCGGC ATCATGAAGA AGGCGTACGA GTTGTCCACG 
TTAACCGGGA CTCAGGTGTT GCTGCTGGTC GCCAGTGAGA CAGGTCACGT GTACACGTTT 
GCCACCAGGA AGCTGCAGCC GATGATCACC TCGGAGACGG GCAAAGCTCT GATCCAGACC 
TGCCTCAACT CTCCGGACTC CCCCCCTCGC TCCGACCCCT CCTCTGACCA GAGGATGAGC 
GCCACGGGCT TCGAGGAGAC CGACCTCACC TACCAGGTCC CTGAGGGGGA GGGGTGCACA 
GAGGTTGCCA AGGATCTGAT CAAACCAGTG TTTACCTTGT CCAACCTGCC CGTCACCACA 
CAGACCAGGC CCCCCTCCTC CTCTTCACCC TCCTCCTCTT CGTCTTCGTC CTCTGTGTCC 
ATGCAGGTGC AAGCCAGTGC CCCCTCCTGG CAGCTACCCT CCTCCACCAA TGGGACGGTG 
CTGAAGACAT CGGCAGGTGT CATGTTTCCT GGAGGCTTCA CCTTGATGCC AGGTGGCTCG 
CTGCCCCCCG GCACACACAC CATCCCCCTC ACTCAGCTGC AGGGCCAGTC TTTGGCCATC 
CAGGGCCCCG TGGCCCCAGG CCACACCGCC ACGCTGCACG CTCCATCCAC ACAGGCGACC 
ACGCTGCTCC ACCTCCCCGC CAAAGTGTCA CTCGCAAGCA CAGGGGGTGG AGTCTCTCAG 
CAGCTGCAGA CTGTCCAGGT TCAGCCCAGC AGTCAGCAGA TCTCCACCAA TCAAAGCAGT 
TCGGACATCC ACAACCCTGC CTCCTCCACA GTCAGTCTTC CTGTCTCCTC CTCCTCTTCC 
TCAGTAGCTG GTCACATGAT GTACCCCGGT GGTCACACGG TGATGTACAC TGCCCAGACG 
CCGTCTCTTA GTGACGGCAG CCTCACCGTC CTCAACACCT TCCCCCCCAC AGGTCATGCC 
CAGTCACATG ACCCGGGCTC TGTCCCGCAG GTCTTCCTCG CCTCCCTCCC TCCGGTCGCT 
GCTCAGATCC CGGTGTCGGC AGTTCAGCTC CATCAGATGG TGATCAGTCA GCAGAGCAGC 
AGCAGTAACC TGACGGAGCT GCAGGTCGTC AGTCTGGACG TCCATCAATC AAAAGACGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019969524.1
CDS	619..2121
Translation	MGTRTGPGLTGSTTGTNSASDTGGGRFEEREISTEVIYSGSDQDSESGDEEDTPGSGGDRRGVKRERSEREVGRQAVPTSGGQSGGYVGVSPGVPGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRSDPSSDQRMSATGFEETDLTYQVPEGEGCTEVAKDLIKPVFTLSNLPVTTQTRPPSSSSPSSSSSSSSVSMQVQASAPSWQLPSSTNGTVLKTSAGVMFPGGFTLMPGGSLPPGTHTIPLTQLQGQSLAIQGPVAPGHTATLHAPSTQATTLLHLPAKVSLASTGGGVSQQLQTVQVQPSSQQISTNQSSSDIHNPASSTVSLPVSSSSSSVAGHMMYPGGHTVMYTAQTPSLSDGSLTVLNTFPPTGHAQSHDPGSVPQVFLASLPPVAAQIPVSAVQLHQMVISQQSSSSNLTELQVVSLDVHQSKDD

Target ORF information:

RefSeq Version	XM_020113965.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus serum response factor-like (LOC109648170), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020113965.1

ORF Insert Sequence:

ATGGGCACCA GAACCGGACC CGGACTCACG GGGAGCACGA CTGGGACCAA CTCAGCCTCT 
GACACCGGGG GGGGGCGGTT CGAGGAGAGA GAGATCAGCA CCGAGGTGAT TTACAGCGGC 
TCCGACCAGG ACTCCGAGTC CGGGGACGAG GAGGACACAC CGGGTTCAGG AGGGGACAGG 
AGAGGGGTGA AGAGGGAGAG GAGCGAGAGG GAGGTCGGCC GTCAGGCAGT GCCCACCTCC 
GGCGGACAGA GCGGGGGTTA CGTCGGGGTC AGTCCCGGGG TGCCAGGGGC CAAACCCGGC 
AAGAAAACCA GAGGAAGAGT CAAGATCAAG ATGGAGTTCA TCGACAACAA ACTGAGGAGG 
TACACGACCT TCAGCAAGAG GAAGACCGGC ATCATGAAGA AGGCGTACGA GTTGTCCACG 
TTAACCGGGA CTCAGGTGTT GCTGCTGGTC GCCAGTGAGA CAGGTCACGT GTACACGTTT 
GCCACCAGGA AGCTGCAGCC GATGATCACC TCGGAGACGG GCAAAGCTCT GATCCAGACC 
TGCCTCAACT CTCCGGACTC CCCCCCTCGC TCCGACCCCT CCTCTGACCA GAGGATGAGC 
GCCACGGGCT TCGAGGAGAC CGACCTCACC TACCAGGTCC CTGAGGGGGA GGGGTGCACA 
GAGGTTGCCA AGGATCTGAT CAAACCAGTG TTTACCTTGT CCAACCTGCC CGTCACCACA 
CAGACCAGGC CCCCCTCCTC CTCTTCACCC TCCTCCTCTT CGTCTTCGTC CTCTGTGTCC 
ATGCAGGTGC AAGCCAGTGC CCCCTCCTGG CAGCTACCCT CCTCCACCAA TGGGACGGTG 
CTGAAGACAT CGGCAGGTGT CATGTTTCCT GGAGGCTTCA CCTTGATGCC AGGTGGCTCG 
CTGCCCCCCG GCACACACAC CATCCCCCTC ACTCAGCTGC AGGGCCAGTC TTTGGCCATC 
CAGGGCCCCG TGGCCCCAGG CCACACCGCC ACGCTGCACG CTCCATCCAC ACAGGCGACC 
ACGCTGCTCC ACCTCCCCGC CAAAGTGTCA CTCGCAAGCA CAGGGGGTGG AGTCTCTCAG 
CAGCTGCAGA CTGTCCAGGT TCAGCCCAGC AGTCAGCAGA TCTCCACCAA TCAAAGCAGT 
TCGGACATCC ACAACCCTGC CTCCTCCACA GTCAGTCTTC CTGTCTCCTC CTCCTCTTCC 
TCAGTAGCTG GTCACATGAT GTACCCCGGT GGTCACACGG TGATGTACAC TGCCCAGACG 
CCGTCTCTTA GTGACGGCAG CCTCACCGTC CTCAACACCT TCCCCCCCAC AGGTCATGCC 
CAGTCACATG ACCCGGGCTC TGTCCCGCAG GTCTTCCTCG CCTCCCTCCC TCCGGTCGCT 
GCTCAGATCC CGGTGTCGGC AGTTCAGCTC CATCAGATGG TGATCAGTCA GCAGAGCAGC 
AGCAGTAACC TGACGGAGCT GCAGGTCGTC AGTCTGGACG TCCATCAATC AAAAGACGAC 
TGA