PFKM cDNA ORF clone, Panthera pardus(leopard)

Gene
Clones
gRNAs

Summary

Gene Symbol	PFKM
Entrez Gene ID	109249031
Full Name	phosphofructokinase, muscle
Gene Type	protein-coding
Organism	Panthera pardus(leopard)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019419170.1	XP_019274715.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X1
XM_019419171.1	XP_019274716.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X2

Pathways from BioSystems
Gene Ontology

The following PFKM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFKM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa215828	XM_019419170.1 Latest version!	Panthera pardus phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPa215829	XM_019419171.1 Latest version!	Panthera pardus phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PFKM ( XM_019419170.1 ) cDNA ORF clone, Panthera pardus(leopard) -> XP_019274715.1 Panthera pardus phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa215828
Clone ID Related Accession (Same CDS sequence)	XM_019419170.1
Accession Version	XM_019419170.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Panthera pardus(leopard)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017619922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Panthera pardus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATAAGG AGGAATTTCA TCTGAAATTT TTCACGTGTG TGATTCAATC TCGCGGGTTA 
GTCAGGACTC CTCAGAGCAC AGGGGGAGAA GCTTCAACTT CCAGCATGCT CATACAAAAG 
CCACCACCTG AGATAGACAA CATGAAGTAT CTACATGCTA CGGATGATCC AGACACTGTG 
GAAGGCATAC CTATTTTGAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATCGCC GTGTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TTACTGGTGC CCGTGTGTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATAACATCAG GGAAGCCACC 
TGGGAGAGTG TTTCGATGAT GCTTCAGCTG GGAGGCACAG TCATTGGAAG TGCCCGGTGC 
AAGGACTTCC GTGAACGAGA GGGACGACTC CGCGCTGCCC ACAACCTGGT GAAGCGTGGG 
ATTACTAATC TGTGTGTCAT CGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTACT GAGTGACCTC CAGAAAGCGG GTAAGATCAC AGCGGAGGAG 
GCTACGAAGT CCAGCTATCT GAATATCGTG GGTTTGGTGG GCTCAATTGA CAATGACTTT 
TGTGGCACTG ATATGACCAT CGGCACTGAT TCCGCCCTGC ACCGGATCAT AGAGATTGTG 
GACGCCATCA CCACCACTGC TCAGAGCCAC CAGAGGACGT TTGTGCTAGA AGTGATGGGC 
CGGCACTGTG GATACCTGGC CCTTGTCACC TCTCTCTCCT GTGGGGCTGA CTGGGTTTTT 
ATTCCTGAGT GTCCACCGGA TGATGACTGG GAGGAACATC TTTGTCGCCG GCTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC CATCGACAAG 
ACTGGGAAAC CCATAACCTC AGAAGACATC AAGAATCTGG TGGTGAAGCG TCTGGGATAT 
GACACCCGGG TCACTGTCTT GGGGCATGTG CAGCGGGGTG GGACACCATC AGCCTTTGAC 
CGGATCCTGG GCAGCAGGAT GGGTGTGGAG GCAGTGATGG CACTTTTGGA AGGGACTCCA 
GACACCCCAG CCTGTGTGGT GAGCCTCTCT GGGAACCAGG CTGTGCGCCT GCCCCTCATG 
GAGTGTGTCC AAATGACCAA AGATGTGACC AAGGCCATGA ACGACAGGAA ATTTGATGAA 
GCCATGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AAGTGTACAA GCTTCTGGCT 
CATGTCAGAC CCCCAGTCTC TAAGGGACCG AGTAGCATAC ACACAGTGGC CGTGATGAAC 
GTGGGGGCCC CAGCCGCAGG CATGAACGCT GCCGTCCGTT CCACCGTGAG GATTGGCCTC 
ATCCAGGGCA ACCGGGTGCT GGTTGTACAT GATGGCTTCG AGGGCCTGGC CAAGGGTCAG 
ATCGAGGAAG CTGGCTGGAG CTATGTTGGG GGCTGGACTG GCCAAGGTGG TTCCAAACTT 
GGGACTAAAA GGACTCTACC CAAGAAGAGC TTTGAGCAGA TCAGTGCCAA CATCACTAAG 
TTTAACATTC AGGGCCTTGT CATCATTGGG GGCTTTGAGG CTTACACAGG GGGCCTGGAA 
CTGATGGAGG GCAGGAAGCT GTTTGACGAG CTGTGCATCC CGTTTGTGGT CATCCCCGCG 
ACGGTCTCCA ACAACGTCCC CGGCTCGGAC TTCAGCGTGG GGGCTGACAC AGCACTCAAT 
ACTATCTGCA CGACCTGTGA CCGCATCAAG CAGTCAGCGG CAGGCACCAA GCGTCGGGTG 
TTTATCATCG AAACGATGGG TGGCTACTGT GGCTACCTGG CCACCATGGC AGGACTGGCA 
GCTGGGGCCG ATGCTGCCTA CATTTTTGAG GAGCCCTTCA CCATTCGGGA CCTGCAGGCA 
AATGTTGAAC ACCTGGTGCA TAAGATGAAA ACCACTGTGA AAAGGGGCTT GGTGTTAAGG 
AATGAGAAGT GCAATGAGAA CTATACCACG GACTTCATCT TCAACCTGTA CTCTGAGGAG 
GGGAAGGGCA TATTTGACAG CAGGAAGAAT GTGCTTGGGC ACATGCAGCA GGGTGGAAGC 
CCAACTCCGT TTGACAGGAA TTTTGCCACT AAGATGGGTG CCAAGGCTAT GAACTGGATG 
TCTGGGAAAA TCAAAGAGAG TTACCGTAAT GGGAGGATCT TCGCCAATAC ACCAGACTCA 
GGCTGTGTTC TGGGGATGCG TAAGAGGGCT CTGGTCTTTC AACCGGTGAC TGAGCTGAAG 
GAGCAGACGG ATTTTGACCA CCGCATCCCC AAGGAGCAGT GGTGGCTGAA GCTGAGGCCC 
ATCCTCAAAA TCCTAGCCAA GTATGAGATT GACTTGGACA CCTCAGAGCA CGCCCACCTG 
GAGCACATCA GCCGGAAGCG ATCTGGAGAA GCTTCTATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019274715.1
CDS	10..2571
Translation	MHKEEFHLKFFTCVIQSRGLVRTPQSTGGEASTSSMLIQKPPPEIDNMKYLHATDDPDTVEGIPILKTEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDNIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIIEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKTGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQMTKDVTKAMNDRKFDEAMKLRGRSFMNNWEVYKLLAHVRPPVSKGPSSIHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKLFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVHKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKEQTDFDHRIPKEQWWLKLRPILKILAKYEIDLDTSEHAHLEHISRKRSGEASI

Target ORF information:

RefSeq Version	XM_019419170.1
Organism	Panthera pardus(leopard)
Definition	Panthera pardus phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019419170.1

ORF Insert Sequence:

ATGCATAAGG AGGAATTTCA TCTGAAATTT TTCACGTGTG TGATTCAATC TCGCGGGTTA 
GTCAGGACTC CTCAGAGCAC AGGGGGAGAA GCTTCAACTT CCAGCATGCT CATACAAAAG 
CCACCACCTG AGATAGACAA CATGAAGTAT CTACATGCTA CGGATGATCC AGACACTGTG 
GAAGGCATAC CTATTTTGAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATCGCC GTGTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TTACTGGTGC CCGTGTGTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATAACATCAG GGAAGCCACC 
TGGGAGAGTG TTTCGATGAT GCTTCAGCTG GGAGGCACAG TCATTGGAAG TGCCCGGTGC 
AAGGACTTCC GTGAACGAGA GGGACGACTC CGCGCTGCCC ACAACCTGGT GAAGCGTGGG 
ATTACTAATC TGTGTGTCAT CGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTACT GAGTGACCTC CAGAAAGCGG GTAAGATCAC AGCGGAGGAG 
GCTACGAAGT CCAGCTATCT GAATATCGTG GGTTTGGTGG GCTCAATTGA CAATGACTTT 
TGTGGCACTG ATATGACCAT CGGCACTGAT TCCGCCCTGC ACCGGATCAT AGAGATTGTG 
GACGCCATCA CCACCACTGC TCAGAGCCAC CAGAGGACGT TTGTGCTAGA AGTGATGGGC 
CGGCACTGTG GATACCTGGC CCTTGTCACC TCTCTCTCCT GTGGGGCTGA CTGGGTTTTT 
ATTCCTGAGT GTCCACCGGA TGATGACTGG GAGGAACATC TTTGTCGCCG GCTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC CATCGACAAG 
ACTGGGAAAC CCATAACCTC AGAAGACATC AAGAATCTGG TGGTGAAGCG TCTGGGATAT 
GACACCCGGG TCACTGTCTT GGGGCATGTG CAGCGGGGTG GGACACCATC AGCCTTTGAC 
CGGATCCTGG GCAGCAGGAT GGGTGTGGAG GCAGTGATGG CACTTTTGGA AGGGACTCCA 
GACACCCCAG CCTGTGTGGT GAGCCTCTCT GGGAACCAGG CTGTGCGCCT GCCCCTCATG 
GAGTGTGTCC AAATGACCAA AGATGTGACC AAGGCCATGA ACGACAGGAA ATTTGATGAA 
GCCATGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AAGTGTACAA GCTTCTGGCT 
CATGTCAGAC CCCCAGTCTC TAAGGGACCG AGTAGCATAC ACACAGTGGC CGTGATGAAC 
GTGGGGGCCC CAGCCGCAGG CATGAACGCT GCCGTCCGTT CCACCGTGAG GATTGGCCTC 
ATCCAGGGCA ACCGGGTGCT GGTTGTACAT GATGGCTTCG AGGGCCTGGC CAAGGGTCAG 
ATCGAGGAAG CTGGCTGGAG CTATGTTGGG GGCTGGACTG GCCAAGGTGG TTCCAAACTT 
GGGACTAAAA GGACTCTACC CAAGAAGAGC TTTGAGCAGA TCAGTGCCAA CATCACTAAG 
TTTAACATTC AGGGCCTTGT CATCATTGGG GGCTTTGAGG CTTACACAGG GGGCCTGGAA 
CTGATGGAGG GCAGGAAGCT GTTTGACGAG CTGTGCATCC CGTTTGTGGT CATCCCCGCG 
ACGGTCTCCA ACAACGTCCC CGGCTCGGAC TTCAGCGTGG GGGCTGACAC AGCACTCAAT 
ACTATCTGCA CGACCTGTGA CCGCATCAAG CAGTCAGCGG CAGGCACCAA GCGTCGGGTG 
TTTATCATCG AAACGATGGG TGGCTACTGT GGCTACCTGG CCACCATGGC AGGACTGGCA 
GCTGGGGCCG ATGCTGCCTA CATTTTTGAG GAGCCCTTCA CCATTCGGGA CCTGCAGGCA 
AATGTTGAAC ACCTGGTGCA TAAGATGAAA ACCACTGTGA AAAGGGGCTT GGTGTTAAGG 
AATGAGAAGT GCAATGAGAA CTATACCACG GACTTCATCT TCAACCTGTA CTCTGAGGAG 
GGGAAGGGCA TATTTGACAG CAGGAAGAAT GTGCTTGGGC ACATGCAGCA GGGTGGAAGC 
CCAACTCCGT TTGACAGGAA TTTTGCCACT AAGATGGGTG CCAAGGCTAT GAACTGGATG 
TCTGGGAAAA TCAAAGAGAG TTACCGTAAT GGGAGGATCT TCGCCAATAC ACCAGACTCA 
GGCTGTGTTC TGGGGATGCG TAAGAGGGCT CTGGTCTTTC AACCGGTGAC TGAGCTGAAG 
GAGCAGACGG ATTTTGACCA CCGCATCCCC AAGGAGCAGT GGTGGCTGAA GCTGAGGCCC 
ATCCTCAAAA TCCTAGCCAA GTATGAGATT GACTTGGACA CCTCAGAGCA CGCCCACCTG 
GAGCACATCA GCCGGAAGCG ATCTGGAGAA GCTTCTATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_019419171.1 ) cDNA ORF clone, Panthera pardus(leopard) -> XP_019274716.1 Panthera pardus phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa215829
Clone ID Related Accession (Same CDS sequence)	XM_019419171.1
Accession Version	XM_019419171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Panthera pardus(leopard)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017619922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Panthera pardus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATCGCCGTG 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTTA CTGGTGCCCG TGTGTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATA ACATCAGGGA AGCCACCTGG GAGAGTGTTT CGATGATGCT TCAGCTGGGA 
GGCACAGTCA TTGGAAGTGC CCGGTGCAAG GACTTCCGTG AACGAGAGGG ACGACTCCGC 
GCTGCCCACA ACCTGGTGAA GCGTGGGATT ACTAATCTGT GTGTCATCGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTACTGAG TGACCTCCAG 
AAAGCGGGTA AGATCACAGC GGAGGAGGCT ACGAAGTCCA GCTATCTGAA TATCGTGGGT 
TTGGTGGGCT CAATTGACAA TGACTTTTGT GGCACTGATA TGACCATCGG CACTGATTCC 
GCCCTGCACC GGATCATAGA GATTGTGGAC GCCATCACCA CCACTGCTCA GAGCCACCAG 
AGGACGTTTG TGCTAGAAGT GATGGGCCGG CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTCTCCTGTG GGGCTGACTG GGTTTTTATT CCTGAGTGTC CACCGGATGA TGACTGGGAG 
GAACATCTTT GTCGCCGGCT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCCAT CGACAAGACT GGGAAACCCA TAACCTCAGA AGACATCAAG 
AATCTGGTGG TGAAGCGTCT GGGATATGAC ACCCGGGTCA CTGTCTTGGG GCATGTGCAG 
CGGGGTGGGA CACCATCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAGGCA 
GTGATGGCAC TTTTGGAAGG GACTCCAGAC ACCCCAGCCT GTGTGGTGAG CCTCTCTGGG 
AACCAGGCTG TGCGCCTGCC CCTCATGGAG TGTGTCCAAA TGACCAAAGA TGTGACCAAG 
GCCATGAACG ACAGGAAATT TGATGAAGCC ATGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAAG TGTACAAGCT TCTGGCTCAT GTCAGACCCC CAGTCTCTAA GGGACCGAGT 
AGCATACACA CAGTGGCCGT GATGAACGTG GGGGCCCCAG CCGCAGGCAT GAACGCTGCC 
GTCCGTTCCA CCGTGAGGAT TGGCCTCATC CAGGGCAACC GGGTGCTGGT TGTACATGAT 
GGCTTCGAGG GCCTGGCCAA GGGTCAGATC GAGGAAGCTG GCTGGAGCTA TGTTGGGGGC 
TGGACTGGCC AAGGTGGTTC CAAACTTGGG ACTAAAAGGA CTCTACCCAA GAAGAGCTTT 
GAGCAGATCA GTGCCAACAT CACTAAGTTT AACATTCAGG GCCTTGTCAT CATTGGGGGC 
TTTGAGGCTT ACACAGGGGG CCTGGAACTG ATGGAGGGCA GGAAGCTGTT TGACGAGCTG 
TGCATCCCGT TTGTGGTCAT CCCCGCGACG GTCTCCAACA ACGTCCCCGG CTCGGACTTC 
AGCGTGGGGG CTGACACAGC ACTCAATACT ATCTGCACGA CCTGTGACCG CATCAAGCAG 
TCAGCGGCAG GCACCAAGCG TCGGGTGTTT ATCATCGAAA CGATGGGTGG CTACTGTGGC 
TACCTGGCCA CCATGGCAGG ACTGGCAGCT GGGGCCGATG CTGCCTACAT TTTTGAGGAG 
CCCTTCACCA TTCGGGACCT GCAGGCAAAT GTTGAACACC TGGTGCATAA GATGAAAACC 
ACTGTGAAAA GGGGCTTGGT GTTAAGGAAT GAGAAGTGCA ATGAGAACTA TACCACGGAC 
TTCATCTTCA ACCTGTACTC TGAGGAGGGG AAGGGCATAT TTGACAGCAG GAAGAATGTG 
CTTGGGCACA TGCAGCAGGG TGGAAGCCCA ACTCCGTTTG ACAGGAATTT TGCCACTAAG 
ATGGGTGCCA AGGCTATGAA CTGGATGTCT GGGAAAATCA AAGAGAGTTA CCGTAATGGG 
AGGATCTTCG CCAATACACC AGACTCAGGC TGTGTTCTGG GGATGCGTAA GAGGGCTCTG 
GTCTTTCAAC CGGTGACTGA GCTGAAGGAG CAGACGGATT TTGACCACCG CATCCCCAAG 
GAGCAGTGGT GGCTGAAGCT GAGGCCCATC CTCAAAATCC TAGCCAAGTA TGAGATTGAC 
TTGGACACCT CAGAGCACGC CCACCTGGAG CACATCAGCC GGAAGCGATC TGGAGAAGCT 
TCTATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019274716.1
CDS	135..2483
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDNIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIIEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKTGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQMTKDVTKAMNDRKFDEAMKLRGRSFMNNWEVYKLLAHVRPPVSKGPSSIHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKLFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVHKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKEQTDFDHRIPKEQWWLKLRPILKILAKYEIDLDTSEHAHLEHISRKRSGEASI

Target ORF information:

RefSeq Version	XM_019419171.1
Organism	Panthera pardus(leopard)
Definition	Panthera pardus phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019419171.1

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATCGCCGTG 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTTA CTGGTGCCCG TGTGTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATA ACATCAGGGA AGCCACCTGG GAGAGTGTTT CGATGATGCT TCAGCTGGGA 
GGCACAGTCA TTGGAAGTGC CCGGTGCAAG GACTTCCGTG AACGAGAGGG ACGACTCCGC 
GCTGCCCACA ACCTGGTGAA GCGTGGGATT ACTAATCTGT GTGTCATCGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTACTGAG TGACCTCCAG 
AAAGCGGGTA AGATCACAGC GGAGGAGGCT ACGAAGTCCA GCTATCTGAA TATCGTGGGT 
TTGGTGGGCT CAATTGACAA TGACTTTTGT GGCACTGATA TGACCATCGG CACTGATTCC 
GCCCTGCACC GGATCATAGA GATTGTGGAC GCCATCACCA CCACTGCTCA GAGCCACCAG 
AGGACGTTTG TGCTAGAAGT GATGGGCCGG CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTCTCCTGTG GGGCTGACTG GGTTTTTATT CCTGAGTGTC CACCGGATGA TGACTGGGAG 
GAACATCTTT GTCGCCGGCT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCCAT CGACAAGACT GGGAAACCCA TAACCTCAGA AGACATCAAG 
AATCTGGTGG TGAAGCGTCT GGGATATGAC ACCCGGGTCA CTGTCTTGGG GCATGTGCAG 
CGGGGTGGGA CACCATCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAGGCA 
GTGATGGCAC TTTTGGAAGG GACTCCAGAC ACCCCAGCCT GTGTGGTGAG CCTCTCTGGG 
AACCAGGCTG TGCGCCTGCC CCTCATGGAG TGTGTCCAAA TGACCAAAGA TGTGACCAAG 
GCCATGAACG ACAGGAAATT TGATGAAGCC ATGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAAG TGTACAAGCT TCTGGCTCAT GTCAGACCCC CAGTCTCTAA GGGACCGAGT 
AGCATACACA CAGTGGCCGT GATGAACGTG GGGGCCCCAG CCGCAGGCAT GAACGCTGCC 
GTCCGTTCCA CCGTGAGGAT TGGCCTCATC CAGGGCAACC GGGTGCTGGT TGTACATGAT 
GGCTTCGAGG GCCTGGCCAA GGGTCAGATC GAGGAAGCTG GCTGGAGCTA TGTTGGGGGC 
TGGACTGGCC AAGGTGGTTC CAAACTTGGG ACTAAAAGGA CTCTACCCAA GAAGAGCTTT 
GAGCAGATCA GTGCCAACAT CACTAAGTTT AACATTCAGG GCCTTGTCAT CATTGGGGGC 
TTTGAGGCTT ACACAGGGGG CCTGGAACTG ATGGAGGGCA GGAAGCTGTT TGACGAGCTG 
TGCATCCCGT TTGTGGTCAT CCCCGCGACG GTCTCCAACA ACGTCCCCGG CTCGGACTTC 
AGCGTGGGGG CTGACACAGC ACTCAATACT ATCTGCACGA CCTGTGACCG CATCAAGCAG 
TCAGCGGCAG GCACCAAGCG TCGGGTGTTT ATCATCGAAA CGATGGGTGG CTACTGTGGC 
TACCTGGCCA CCATGGCAGG ACTGGCAGCT GGGGCCGATG CTGCCTACAT TTTTGAGGAG 
CCCTTCACCA TTCGGGACCT GCAGGCAAAT GTTGAACACC TGGTGCATAA GATGAAAACC 
ACTGTGAAAA GGGGCTTGGT GTTAAGGAAT GAGAAGTGCA ATGAGAACTA TACCACGGAC 
TTCATCTTCA ACCTGTACTC TGAGGAGGGG AAGGGCATAT TTGACAGCAG GAAGAATGTG 
CTTGGGCACA TGCAGCAGGG TGGAAGCCCA ACTCCGTTTG ACAGGAATTT TGCCACTAAG 
ATGGGTGCCA AGGCTATGAA CTGGATGTCT GGGAAAATCA AAGAGAGTTA CCGTAATGGG 
AGGATCTTCG CCAATACACC AGACTCAGGC TGTGTTCTGG GGATGCGTAA GAGGGCTCTG 
GTCTTTCAAC CGGTGACTGA GCTGAAGGAG CAGACGGATT TTGACCACCG CATCCCCAAG 
GAGCAGTGGT GGCTGAAGCT GAGGCCCATC CTCAAAATCC TAGCCAAGTA TGAGATTGAC 
TTGGACACCT CAGAGCACGC CCACCTGGAG CACATCAGCC GGAAGCGATC TGGAGAAGCT 
TCTATCTAA