PIAS4 cDNA ORF clone, Pan paniscus(pygmy chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIAS4
Entrez Gene ID	100994639
Full Name	protein inhibitor of activated STAT 4
Gene Type	protein-coding
Organism	Pan paniscus(pygmy chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003819270.4	XP_003819318.1	LOW QUALITY PROTEIN: E3 SUMO-protein ligase PIAS4
XM_003819270.3	XP_003819318.1	LOW QUALITY PROTEIN: E3 SUMO-protein ligase PIAS4

Pathways from BioSystems
Gene Ontology

The following PIAS4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIAS4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa70928	XM_003819270.4 Latest version!	Pan paniscus protein inhibitor of activated STAT 4 (PIAS4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
View Old Accession Versions >>
OPa70928	XM_003819270.3	Pan paniscus protein inhibitor of activated STAT, 4 (PIAS4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIAS4 ( XM_003819270.4 ) cDNA ORF clone, Pan paniscus(pygmy chimpanzee) -> XP_003819318.1 Pan paniscus protein inhibitor of activated STAT 4 (PIAS4), mRNA.

Price & Availability↑

CloneID	OPa70928
Clone ID Related Accession (Same CDS sequence)	XM_003819270.4 , XM_003819270.3
Accession Version	XM_003819270.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-30
Organism	Pan paniscus(pygmy chimpanzee)
Product	LOW QUALITY PROTEIN: E3 SUMO-protein ligase PIAS4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_027887.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 1, 2018 this sequence version replaced XM_003819270.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan paniscus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGACATCGT CTCTCCCTGC TAAGGCTTTG GGCCGCTTCC AGAAGAAACA TCTTGTCCGC 
AACCCTTTTT GTTTTCTGCA CAAGAACATG GTGATGAGTT TTCGAGTGTC CGACCTTCAG 
ATGCTCCTGG GTTTTGTGGG CCGGAGTAAG AGTGGACTGA AGCACGAGCT CGTCACCAGG 
GCCCTCCAGC TGGTGCAGTT TGACTGTAGC CCTGAGCTGT TCAAGAAGAT CAAGGAGCTG 
TACGAGACCC GCTACGCCAA GAAGAACTCG GAGCCTGCCC CACAGCCGCA CCGGCCCCTG 
GACCCCCTGA CCATGCACTC CACCTACGAC CGGGCCGGCG CTGTGCCCAG GACTCCGCTG 
GCAGGCCCCA ATATTGACTA CCCCGTGCTC TACGGAAAGT ACTTAAACGG ACTGGGACGG 
TTGCCCGCCA AGACCCTCAA GCCAGAAGTC CGCCTGGTGA AGCTGCCGTT CTTTAATATG 
CTGGACGAGC TGCTGAAGCC CACCGAATTA GTCCCACAGA ACAACGAGAA GCTTCAGGAG 
AGCCCGTGCA TCTTCGCATT GACGCCAAGA CAGGTGGAGT TGATCCGGAA CTCCAGGGAA 
CTGCAGCCCG GAGTTAAAGC CGTGCAGGTC GTCCTGAGAA TCTGTTACTC AGACACCAGC 
TGCCCTCAGG AGGACCAGTA CCCGCCCAAC ATCGCTGTGA AGGTCAACCA CAGCTACTGC 
TCGGTCCCGG GCTACTACCC CTCCAATAAG CCCGGGGTGG AGCCCAAGAG GCCGTGCCGC 
CCCATCAACC TCACCCACCT CATGTACCTG TCCTCGGCCA CCAACCGCAT CACCGTCACC 
TGGGGGAACT ACGGCAAGAG CTACTCGGTG GCCCTGTACC TGGTGCGGCA GCTGACCTCA 
TCGGAGCTGC TGCAGAGGCT GAAGACCATT GGGGTAAAGC ACCCGGAGCT GTGCAAGGCA 
CTGGTCAAGG AGAAGCTGCG CCTTGATCCT GACAGCGAGA TCGCCACCAC CGGTGTGCGG 
GTGTCCCTCA TCTGTCCGCT GGTGAAGATG CGGCTCTCCG TGCCCTGCCG GGCAGAGACC 
TGCGCCCACC TGCAGTGCTT CGACGCCGTC TTCTACCTGC AGATGAACGA GAAGAAGCCC 
ACCTGGATGT GCCCCGTGTG CGACAAGCCA GCCCCCTACG ACCAGCTCAT CATCGACGGG 
CTCCTCTCGA AGATTCTGAG CGAGTGTGAG GACGCCGACG AGATCGAGTA CCTGGTGGAC 
GGCTCGTGGT GCCCGATCCG CGCCGAAAAG GAGCGCAGCT GCAGCCCGCA GGGCGCCATC 
CTCGTGCTGG GCCCCTCGGA CGCCAATGGG CTCCTGCCCG CCCCCAGCGT CAACGGGAGC 
GGTGCCCTGG GCAGCACGGG TGGCGGCGGC CCGGTGGGCA GCGTGGAGAA TGGGAAGCCG 
GGCGCCGATG TGGTGGACCT CACGCTGGAC AGCTCATCGT CCTCGGAGGA GGAGGAGGAG 
GAGGAGGAAG AGGAGGAGGA AGACGAGGAC GAAGAGGGGC CCCGGCCANA GCGCCGCTGC 
CCCTTCCAGA AGGGCCTGGT GCCGGCCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003819318.1
CDS	16..1608
Translation	MTSSLPAKALGRFQKKHLVRNPFCFLHKNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKIKELYETRYAKKNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDYPVLYGKYLNGLGRLPAKTLKPEVRLVKLPFFNMLDELLKPTELVPQNNEKLQESPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQYPPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIRAEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSVENGKPGADVVDLTLDSSSSSEEEEEEEEEEEEDEDEEGPRPnullRRCPFQKGLVPAC

Target ORF information:

RefSeq Version	XM_003819270.4
Organism	Pan paniscus(pygmy chimpanzee)
Definition	Pan paniscus protein inhibitor of activated STAT 4 (PIAS4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003819270.4

ORF Insert Sequence:

ATGACATCGT CTCTCCCTGC TAAGGCTTTG GGCCGCTTCC AGAAGAAACA TCTTGTCCGC 
AACCCTTTTT GTTTTCTGCA CAAGAACATG GTGATGAGTT TTCGAGTGTC CGACCTTCAG 
ATGCTCCTGG GTTTTGTGGG CCGGAGTAAG AGTGGACTGA AGCACGAGCT CGTCACCAGG 
GCCCTCCAGC TGGTGCAGTT TGACTGTAGC CCTGAGCTGT TCAAGAAGAT CAAGGAGCTG 
TACGAGACCC GCTACGCCAA GAAGAACTCG GAGCCTGCCC CACAGCCGCA CCGGCCCCTG 
GACCCCCTGA CCATGCACTC CACCTACGAC CGGGCCGGCG CTGTGCCCAG GACTCCGCTG 
GCAGGCCCCA ATATTGACTA CCCCGTGCTC TACGGAAAGT ACTTAAACGG ACTGGGACGG 
TTGCCCGCCA AGACCCTCAA GCCAGAAGTC CGCCTGGTGA AGCTGCCGTT CTTTAATATG 
CTGGACGAGC TGCTGAAGCC CACCGAATTA GTCCCACAGA ACAACGAGAA GCTTCAGGAG 
AGCCCGTGCA TCTTCGCATT GACGCCAAGA CAGGTGGAGT TGATCCGGAA CTCCAGGGAA 
CTGCAGCCCG GAGTTAAAGC CGTGCAGGTC GTCCTGAGAA TCTGTTACTC AGACACCAGC 
TGCCCTCAGG AGGACCAGTA CCCGCCCAAC ATCGCTGTGA AGGTCAACCA CAGCTACTGC 
TCGGTCCCGG GCTACTACCC CTCCAATAAG CCCGGGGTGG AGCCCAAGAG GCCGTGCCGC 
CCCATCAACC TCACCCACCT CATGTACCTG TCCTCGGCCA CCAACCGCAT CACCGTCACC 
TGGGGGAACT ACGGCAAGAG CTACTCGGTG GCCCTGTACC TGGTGCGGCA GCTGACCTCA 
TCGGAGCTGC TGCAGAGGCT GAAGACCATT GGGGTAAAGC ACCCGGAGCT GTGCAAGGCA 
CTGGTCAAGG AGAAGCTGCG CCTTGATCCT GACAGCGAGA TCGCCACCAC CGGTGTGCGG 
GTGTCCCTCA TCTGTCCGCT GGTGAAGATG CGGCTCTCCG TGCCCTGCCG GGCAGAGACC 
TGCGCCCACC TGCAGTGCTT CGACGCCGTC TTCTACCTGC AGATGAACGA GAAGAAGCCC 
ACCTGGATGT GCCCCGTGTG CGACAAGCCA GCCCCCTACG ACCAGCTCAT CATCGACGGG 
CTCCTCTCGA AGATTCTGAG CGAGTGTGAG GACGCCGACG AGATCGAGTA CCTGGTGGAC 
GGCTCGTGGT GCCCGATCCG CGCCGAAAAG GAGCGCAGCT GCAGCCCGCA GGGCGCCATC 
CTCGTGCTGG GCCCCTCGGA CGCCAATGGG CTCCTGCCCG CCCCCAGCGT CAACGGGAGC 
GGTGCCCTGG GCAGCACGGG TGGCGGCGGC CCGGTGGGCA GCGTGGAGAA TGGGAAGCCG 
GGCGCCGATG TGGTGGACCT CACGCTGGAC AGCTCATCGT CCTCGGAGGA GGAGGAGGAG 
GAGGAGGAAG AGGAGGAGGA AGACGAGGAC GAAGAGGGGC CCCGGCCANA GCGCCGCTGC 
CCCTTCCAGA AGGGCCTGGT GCCGGCCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIAS4 ( XM_003819270.3 ) cDNA ORF clone, Pan paniscus(pygmy chimpanzee) -> XP_003819318.1 Pan paniscus protein inhibitor of activated STAT, 4 (PIAS4), mRNA.

Price & Availability↑

CloneID	OPa70928
Clone ID Related Accession (Same CDS sequence)	XM_003819270.4 , XM_003819270.3
Accession Version	XM_003819270.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-29
Organism	Pan paniscus(pygmy chimpanzee)
Product	LOW QUALITY PROTEIN: E3 SUMO-protein ligase PIAS4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014014343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 30, 2015 this sequence version replaced XM_003819270.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan paniscus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGACATCGT CTCTCCCTGC TAAGGCTTTG GGCCGCTTCC AGAAGAAACA TCTTGTCCGC 
AACCCTTTTT GTTTTCTGCA CAAGAACATG GTGATGAGTT TTCGAGTGTC CGACCTTCAG 
ATGCTCCTGG GTTTTGTGGG CCGGAGTAAG AGTGGACTGA AGCACGAGCT CGTCACCAGG 
GCCCTCCAGC TGGTGCAGTT TGACTGTAGC CCTGAGCTGT TCAAGAAGAT CAAGGAGCTG 
TACGAGACCC GCTACGCCAA GAAGAACTCG GAGCCTGCCC CACAGCCGCA CCGGCCCCTG 
GACCCCCTGA CCATGCACTC CACCTACGAC CGGGCCGGCG CTGTGCCCAG GACTCCGCTG 
GCAGGCCCCA ATATTGACTA CCCCGTGCTC TACGGAAAGT ACTTAAACGG ACTGGGACGG 
TTGCCCGCCA AGACCCTCAA GCCAGAAGTC CGCCTGGTGA AGCTGCCGTT CTTTAATATG 
CTGGACGAGC TGCTGAAGCC CACCGAATTA GTCCCACAGA ACAACGAGAA GCTTCAGGAG 
AGCCCGTGCA TCTTCGCATT GACGCCAAGA CAGGTGGAGT TGATCCGGAA CTCCAGGGAA 
CTGCAGCCCG GAGTTAAAGC CGTGCAGGTC GTCCTGAGAA TCTGTTACTC AGACACCAGC 
TGCCCTCAGG AGGACCAGTA CCCGCCCAAC ATCGCTGTGA AGGTCAACCA CAGCTACTGC 
TCGGTCCCGG GCTACTACCC CTCCAATAAG CCCGGGGTGG AGCCCAAGAG GCCGTGCCGC 
CCCATCAACC TCACCCACCT CATGTACCTG TCCTCGGCCA CCAACCGCAT CACCGTCACC 
TGGGGGAACT ACGGCAAGAG CTACTCGGTG GCCCTGTACC TGGTGCGGCA GCTGACCTCA 
TCGGAGCTGC TGCAGAGGCT GAAGACCATT GGGGTAAAGC ACCCGGAGCT GTGCAAGGCA 
CTGGTCAAGG AGAAGCTGCG CCTTGATCCT GACAGCGAGA TCGCCACCAC CGGTGTGCGG 
GTGTCCCTCA TCTGTCCGCT GGTGAAGATG CGGCTCTCCG TGCCCTGCCG GGCAGAGACC 
TGCGCCCACC TGCAGTGCTT CGACGCCGTC TTCTACCTGC AGATGAACGA GAAGAAGCCC 
ACCTGGATGT GCCCCGTGTG CGACAAGCCA GCCCCCTACG ACCAGCTCAT CATCGACGGG 
CTCCTCTCGA AGATTCTGAG CGAGTGTGAG GACGCCGACG AGATCGAGTA CCTGGTGGAC 
GGCTCGTGGT GCCCGATCCG CGCCGAAAAG GAGCGCAGCT GCAGCCCGCA GGGCGCCATC 
CTCGTGCTGG GCCCCTCGGA CGCCAATGGG CTCCTGCCCG CCCCCAGCGT CAACGGGAGC 
GGTGCCCTGG GCAGCACGGG TGGCGGCGGC CCGGTGGGCA GCGTGGAGAA TGGGAAGCCG 
GGCGCCGATG TGGTGGACCT CACGCTGGAC AGCTCATCGT CCTCGGAGGA GGAGGAGGAG 
GAGGAGGAAG AGGAGGAGGA AGACGAGGAC GAAGAGGGGC CCCGGCCANA GCGCCGCTGC 
CCCTTCCAGA AGGGCCTGGT GCCGGCCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003819318.1
CDS	35..1627
Translation	MTSSLPAKALGRFQKKHLVRNPFCFLHKNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKIKELYETRYAKKNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDYPVLYGKYLNGLGRLPAKTLKPEVRLVKLPFFNMLDELLKPTELVPQNNEKLQESPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQYPPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIRAEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSVENGKPGADVVDLTLDSSSSSEEEEEEEEEEEEDEDEEGPRPnullRRCPFQKGLVPAC

Target ORF information:

RefSeq Version	XM_003819270.3
Organism	Pan paniscus(pygmy chimpanzee)
Definition	Pan paniscus protein inhibitor of activated STAT, 4 (PIAS4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003819270.3

ORF Insert Sequence:

ATGACATCGT CTCTCCCTGC TAAGGCTTTG GGCCGCTTCC AGAAGAAACA TCTTGTCCGC 
AACCCTTTTT GTTTTCTGCA CAAGAACATG GTGATGAGTT TTCGAGTGTC CGACCTTCAG 
ATGCTCCTGG GTTTTGTGGG CCGGAGTAAG AGTGGACTGA AGCACGAGCT CGTCACCAGG 
GCCCTCCAGC TGGTGCAGTT TGACTGTAGC CCTGAGCTGT TCAAGAAGAT CAAGGAGCTG 
TACGAGACCC GCTACGCCAA GAAGAACTCG GAGCCTGCCC CACAGCCGCA CCGGCCCCTG 
GACCCCCTGA CCATGCACTC CACCTACGAC CGGGCCGGCG CTGTGCCCAG GACTCCGCTG 
GCAGGCCCCA ATATTGACTA CCCCGTGCTC TACGGAAAGT ACTTAAACGG ACTGGGACGG 
TTGCCCGCCA AGACCCTCAA GCCAGAAGTC CGCCTGGTGA AGCTGCCGTT CTTTAATATG 
CTGGACGAGC TGCTGAAGCC CACCGAATTA GTCCCACAGA ACAACGAGAA GCTTCAGGAG 
AGCCCGTGCA TCTTCGCATT GACGCCAAGA CAGGTGGAGT TGATCCGGAA CTCCAGGGAA 
CTGCAGCCCG GAGTTAAAGC CGTGCAGGTC GTCCTGAGAA TCTGTTACTC AGACACCAGC 
TGCCCTCAGG AGGACCAGTA CCCGCCCAAC ATCGCTGTGA AGGTCAACCA CAGCTACTGC 
TCGGTCCCGG GCTACTACCC CTCCAATAAG CCCGGGGTGG AGCCCAAGAG GCCGTGCCGC 
CCCATCAACC TCACCCACCT CATGTACCTG TCCTCGGCCA CCAACCGCAT CACCGTCACC 
TGGGGGAACT ACGGCAAGAG CTACTCGGTG GCCCTGTACC TGGTGCGGCA GCTGACCTCA 
TCGGAGCTGC TGCAGAGGCT GAAGACCATT GGGGTAAAGC ACCCGGAGCT GTGCAAGGCA 
CTGGTCAAGG AGAAGCTGCG CCTTGATCCT GACAGCGAGA TCGCCACCAC CGGTGTGCGG 
GTGTCCCTCA TCTGTCCGCT GGTGAAGATG CGGCTCTCCG TGCCCTGCCG GGCAGAGACC 
TGCGCCCACC TGCAGTGCTT CGACGCCGTC TTCTACCTGC AGATGAACGA GAAGAAGCCC 
ACCTGGATGT GCCCCGTGTG CGACAAGCCA GCCCCCTACG ACCAGCTCAT CATCGACGGG 
CTCCTCTCGA AGATTCTGAG CGAGTGTGAG GACGCCGACG AGATCGAGTA CCTGGTGGAC 
GGCTCGTGGT GCCCGATCCG CGCCGAAAAG GAGCGCAGCT GCAGCCCGCA GGGCGCCATC 
CTCGTGCTGG GCCCCTCGGA CGCCAATGGG CTCCTGCCCG CCCCCAGCGT CAACGGGAGC 
GGTGCCCTGG GCAGCACGGG TGGCGGCGGC CCGGTGGGCA GCGTGGAGAA TGGGAAGCCG 
GGCGCCGATG TGGTGGACCT CACGCTGGAC AGCTCATCGT CCTCGGAGGA GGAGGAGGAG 
GAGGAGGAAG AGGAGGAGGA AGACGAGGAC GAAGAGGGGC CCCGGCCANA GCGCCGCTGC 
CCCTTCCAGA AGGGCCTGGT GCCGGCCTGC TGA