TLR7 cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	TLR7
Entrez Gene ID	554254
Full Name	toll like receptor 7
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001135059.1	NP_001128531.1	toll-like receptor 7 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The double-stranded RNA bluetongue virus induces type I interferon in plasmacytoid dendritic cells via a MYD88-dependent TLR7/8-independent signaling pathway.
Ruscanu S, Pascale F, Bourge M, Hemati B, Elhmouzi-Younes J, Urien C, Bonneau M, Takamatsu H, Hope J, Mertens P, Meyer G, Stewart M, Roy P, Meurs EF, Dabo S, Zientara S, Breard E, Sailleau C, Chauveau E, Vitour D, Charley B, Schwartz-Cornil I
Journal of virology86(10)5817-28(2012 May)

Characterization of ovine TLR7 and TLR8 protein coding regions, detection of mutations and Maedi Visna virus infection.
Mikula I, Bhide M, Pastorekova S, Mikula I
Veterinary immunology and immunopathology138(1-2)51-9(2010 Nov)

Molecular cloning and characterization of Toll-like receptors 1-10 in sheep.
Chang JS, Russell GC, Jann O, Glass EJ, Werling D, Haig DM
Veterinary immunology and immunopathology127(1-2)94-105(2009 Jan)

Differential expression of pattern recognition receptors in sheep tissues and leukocyte subsets.
Nalubamba KS, Gossner AG, Dalziel RG, Hopkins J
Veterinary immunology and immunopathology118(3-4)252-62(2007 Aug)

Identification and expression of Toll-like receptors 1-10 in selected bovine and ovine tissues.
Menzies M, Ingham A
Veterinary immunology and immunopathology109(1-2)23-30(2006 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bluetongue virus induces IFN-alpha/beta in plasmacytoid dendritic cells via a MYD88-dependent TLR7/8-independent signaling pathway.
Title: The double-stranded RNA bluetongue virus induces type I interferon in plasmacytoid dendritic cells via a MYD88-dependent TLR7/8-independent signaling pathway.

Three mutations were localized in extra LRR (leucine rich repeat) region of TLR7, while mutation Ile73Leu (P-0.498) was located within LRR2 motif. Mutations in TLR7 may play an important role as predisposition factor for Maedi Visna infection.
Title: Characterization of ovine TLR7 and TLR8 protein coding regions, detection of mutations and Maedi Visna virus infection.

The following TLR7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLR7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSm21288	NM_001135059.1 Latest version!	Ovis aries toll like receptor 7 (TLR7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLR7 ( NM_001135059.1 ) cDNA ORF clone, Ovis aries(sheep) -> NP_001128531.1 Ovis aries toll like receptor 7 (TLR7), mRNA.

Price & Availability↑

CloneID	OSm21288
Clone ID Related Accession (Same CDS sequence)	NM_001135059.1
Accession Version	NM_001135059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Ovis aries(sheep)
Product	toll-like receptor 7 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AM981305.1. ##Evidence-Data-START## Transcript exon combination :: AM981305.1 [ECO:0000332] ##Evidence-Data-END##

ATGTGGACAT TGAAGAGACA GTTTCCTATC CTTTTTAACA TGATCCTAAT TTCTGAACTC 
CTTGGGGCTA GATGGTTTCC TAAAACTCTG CCCTGTGATG TCACTCTGGA TGCTCCAAAT 
GCCCATGTGA TTGTGGACTG CACAGACAAA CATTTGACAG AAATTCCTGG AGGTATTCCT 
GCCAATGCCA CCAACCTCAC CCTCACCATT AACCACATAG CAGGCATCTC TCCAGCCTCC 
TTCCACCGGC TGGACCATCT GGTGGAGATT GATTTCAGGT GCAACTGCAT ACCTGTTCGA 
CTGGGGCCAA AAGACAACGT GTGCACCAAA AGGCTACAGA TTGAACCCAG CAGCTTTAGC 
AAACTCACAT ATTTAAAATC TCTTTACCTG GATGGAAACC AGCTTCTAGA AATACCTCAG 
GATCTTCCTC CCAGCTTACA GCTGCTGAGC CTGGAGGCCA ACAACATCTT CTTGATCATG 
AAGGAGAATC TAACAGAACT GGCCAACCTA GAAATACTCT ACCTGGGCCA AAACTGTTAC 
TATCGTAACC CTTGTAATGT TTCATTTACT ATCGAAAAAG ATGCTTTCCT AAATATGAGA 
AATTTAAAAT TGCTCTCCCT AAAAGATAAC AATATCTCAG CTGTCCCCAC TGTTTTGCCA 
TCTAGTTTGA CAGAACTCTA TCTTTACAAT AATATCATTA CAAAAATCCA AGAAGATGAT 
TTTAATAACC TCAGTCAACT ACAAGTTCTT GATCTGAGTG GAAATTGCCC TCGTTGTTAT 
AATGTTCCAT TTCCTTGCAC ACCGTGTGAA AATAATTCTC CCCTACAGAT TGATCTAAAT 
GCTTTTGATG CATTGACAGA ATTGCAAGTC TTACGTCTAC ACAGTAACTC TCTTCAGCAT 
GTGCCCCAAA GATGGTTTAA AAACATTAAC AAACTTAAAG AACTAGATCT TTCCCAAAAC 
TTCTTGGCCA AAGAAATTGG GGATGCCAAA TTTTTACATC TTCTTCATAA CCTTGTCAAC 
TTGGATCTGT CTTTCAATTA TGATCTTCAG GTCTACCATG CAGTTATAAA TCTATCAGAT 
GCATTTTCTT CACTGAAAAA ATTGAAAGTT TTGCGAATCA AAGGCTATGT CTTTAAAGAG 
CTGAATAGTT TAAACCTCTT CCCATTACGT AATCTTCCCA GTCTTGAAGT TCTTGATCTT 
GGCACTAACT TTATTAAAAT TGCTGACCTC AGCATATTTA AACAATTTAA AACATTGAAA 
TTCATTGATC TTTCAGTGAA TAAAATATCA CCTTCGGGAG ATTCAACTGA AGGTGGTTTC 
TGCTCTAACA TGAGAACTTC TGTAGAAGGC CATGGGCCCC AGGTCCTTGA AGCATTGCAT 
TATTTCAGAT ATGATGAGTA TGCAAGGAGT TGCCGGTCCA AGAGCAAAGA GCCTCCTTCT 
TTCTTACCTC TTAATGAAGA TTGTTATATG TATGGGCAGA CCTTGGACCT TAGTAGAAAT 
AATATATTTT TTATCAAGTC TTCTGATTTC CAGCATCTTT CTTTCCTCAA ATGCCTAAAC 
TTATCAGGAA ATAGCATTAG CCAGACGCTT AATGGAAGTG AATTTCAGCC TTTAGTGGAG 
TTGAAATATT TGGACTTCTC TAACAATCGG CTTGATTTAC TCTACTCAAC GGCATTTGAG 
GAGCTGCACA ACCTGGAAGT CCTAGATATA AGTAGTAACA GCCATTATTT TCAATCAGAA 
GGAATTACTC ACATGCTAAA TTTTACCAAG AACCTCAAGG TTCTGAAGAA ACTGATGATG 
AACTATAATG ACATCGCTAC CTCCACCAGC AGGACCATGG AGAGTGAATC TCTTCAAATC 
CTGGAGTTCA GAGGCAACCA TTTGGATATT TTATGGAGAG ATGGTGATAA CAGATACTTA 
AAATTCTTTA AGAATCTGCT AAACTTAGAA GAGCTAGACA TCTCTGAAAA TTCTCTGAGT 
TTCTTACCTT TGGGAGTTTT TGATAGTATG CCTCCAAATC TAAAGACTCT CTCCTTGGCC 
AAAAATGGGC TCAAGTCTTT CAGTTGGAAA GGACTACAGA GTCTGAAGAA TCTAGAAACT 
TTGGACCTCA GCTTCAACCA GCTGAAGACT GTCCCTGAGA GATTATCCAA CTGTTCCCGC 
AGCCTCAAGA AACTCATACT TAAGAATAAT CAAATCAGGT GCCTGACAAA GTATTTTCTC 
CAAGGTGCTT TCCAGTTGCG ACATCTGGAC CTCAGCTCAA ATAAAATTCA GGTTATCCAA 
AAGACGAGTT TTCCAGAAAA TGTCCTCAAC AATCTGAACA TTTTGTTTTT GCATCACAAT 
CGATTTCTGT GCAACTGTGA TGCTGTGTGG TTTGTCTGGT GGGTTAACCA TACCGAGGTG 
ACTATTCCTT ACTTGGCCAC AGATGTGACT TGCATGGGAC CAGGAGCACA CAAGGGCCAG 
AGTGTGGTCT CTCTGGATCT ATATACCTGT GAGTTAGATT TGACTAACTT CATCCTGTTC 
TCACTTTCCA TATCAGCAGT TCTCTCTCTG ATGATGATCA CAACAGCAAA CCATCTCTAT 
TTCTGGGATG TGTGGTATAG TTATCATTTC TGTAAAGCCA AAATAAAAGG GTATCGACGT 
CTGATATCAC CCAATTCTTG CTATGATGCT TTCATTGTAT ATGACACTAA AGACCCAGCA 
GTGACAGAGT GGGTTTTGGA TGAGCTGGTG GCCAAATTGG AAGACCCAAG AGAGAAGTGT 
TTTAATTTAT GTCTTGAGGA AAGAGACTGG TTACCAGGGC AGCCTGTTCT GGAAAATCTT 
TCCCAGAGCA TACAGCTTAG CAAAAAGACA GTGTTTGTGA TGACAGACAA GTACGCAAAG 
ACTGAGAATT TTAAGATAGC ATTTTACTTA TCCCATCAGA GGCTCATGGA TGAAAAAGTG 
GATGTAATCA TCTTGATATT CCTTGAGAAG CCCCTTCAGA AGTCCAAGTT TCTCCAACTC 
CGGAAGAGGC TCTGTGGCAG TTCTGTCCTT GAGTGGCCAA CAAACCCACA GGCTCACCCG 
TACTTCTGGC AGTGTCTGAA AAATGCCCTG GCCACAGACA ATCACGTGAC CTACAGTCAG 
GTGTTCAAAG AGACAGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128531.1
CDS	37..3177
Translation	MWTLKRQFPILFNMILISELLGARWFPKTLPCDVTLDAPNAHVIVDCTDKHLTEIPGGIPANATNLTLTINHIAGISPASFHRLDHLVEIDFRCNCIPVRLGPKDNVCTKRLQIEPSSFSKLTYLKSLYLDGNQLLEIPQDLPPSLQLLSLEANNIFLIMKENLTELANLEILYLGQNCYYRNPCNVSFTIEKDAFLNMRNLKLLSLKDNNISAVPTVLPSSLTELYLYNNIITKIQEDDFNNLSQLQVLDLSGNCPRCYNVPFPCTPCENNSPLQIDLNAFDALTELQVLRLHSNSLQHVPQRWFKNINKLKELDLSQNFLAKEIGDAKFLHLLHNLVNLDLSFNYDLQVYHAVINLSDAFSSLKKLKVLRIKGYVFKELNSLNLFPLRNLPSLEVLDLGTNFIKIADLSIFKQFKTLKFIDLSVNKISPSGDSTEGGFCSNMRTSVEGHGPQVLEALHYFRYDEYARSCRSKSKEPPSFLPLNEDCYMYGQTLDLSRNNIFFIKSSDFQHLSFLKCLNLSGNSISQTLNGSEFQPLVELKYLDFSNNRLDLLYSTAFEELHNLEVLDISSNSHYFQSEGITHMLNFTKNLKVLKKLMMNYNDIATSTSRTMESESLQILEFRGNHLDILWRDGDNRYLKFFKNLLNLEELDISENSLSFLPLGVFDSMPPNLKTLSLAKNGLKSFSWKGLQSLKNLETLDLSFNQLKTVPERLSNCSRSLKKLILKNNQIRCLTKYFLQGAFQLRHLDLSSNKIQVIQKTSFPENVLNNLNILFLHHNRFLCNCDAVWFVWWVNHTEVTIPYLATDVTCMGPGAHKGQSVVSLDLYTCELDLTNFILFSLSISAVLSLMMITTANHLYFWDVWYSYHFCKAKIKGYRRLISPNSCYDAFIVYDTKDPAVTEWVLDELVAKLEDPREKCFNLCLEERDWLPGQPVLENLSQSIQLSKKTVFVMTDKYAKTENFKIAFYLSHQRLMDEKVDVIILIFLEKPLQKSKFLQLRKRLCGSSVLEWPTNPQAHPYFWQCLKNALATDNHVTYSQVFKETA

Target ORF information:

RefSeq Version	NM_001135059.1
Organism	Ovis aries(sheep)
Definition	Ovis aries toll like receptor 7 (TLR7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135059.1

ORF Insert Sequence:

ATGTGGACAT TGAAGAGACA GTTTCCTATC CTTTTTAACA TGATCCTAAT TTCTGAACTC 
CTTGGGGCTA GATGGTTTCC TAAAACTCTG CCCTGTGATG TCACTCTGGA TGCTCCAAAT 
GCCCATGTGA TTGTGGACTG CACAGACAAA CATTTGACAG AAATTCCTGG AGGTATTCCT 
GCCAATGCCA CCAACCTCAC CCTCACCATT AACCACATAG CAGGCATCTC TCCAGCCTCC 
TTCCACCGGC TGGACCATCT GGTGGAGATT GATTTCAGGT GCAACTGCAT ACCTGTTCGA 
CTGGGGCCAA AAGACAACGT GTGCACCAAA AGGCTACAGA TTGAACCCAG CAGCTTTAGC 
AAACTCACAT ATTTAAAATC TCTTTACCTG GATGGAAACC AGCTTCTAGA AATACCTCAG 
GATCTTCCTC CCAGCTTACA GCTGCTGAGC CTGGAGGCCA ACAACATCTT CTTGATCATG 
AAGGAGAATC TAACAGAACT GGCCAACCTA GAAATACTCT ACCTGGGCCA AAACTGTTAC 
TATCGTAACC CTTGTAATGT TTCATTTACT ATCGAAAAAG ATGCTTTCCT AAATATGAGA 
AATTTAAAAT TGCTCTCCCT AAAAGATAAC AATATCTCAG CTGTCCCCAC TGTTTTGCCA 
TCTAGTTTGA CAGAACTCTA TCTTTACAAT AATATCATTA CAAAAATCCA AGAAGATGAT 
TTTAATAACC TCAGTCAACT ACAAGTTCTT GATCTGAGTG GAAATTGCCC TCGTTGTTAT 
AATGTTCCAT TTCCTTGCAC ACCGTGTGAA AATAATTCTC CCCTACAGAT TGATCTAAAT 
GCTTTTGATG CATTGACAGA ATTGCAAGTC TTACGTCTAC ACAGTAACTC TCTTCAGCAT 
GTGCCCCAAA GATGGTTTAA AAACATTAAC AAACTTAAAG AACTAGATCT TTCCCAAAAC 
TTCTTGGCCA AAGAAATTGG GGATGCCAAA TTTTTACATC TTCTTCATAA CCTTGTCAAC 
TTGGATCTGT CTTTCAATTA TGATCTTCAG GTCTACCATG CAGTTATAAA TCTATCAGAT 
GCATTTTCTT CACTGAAAAA ATTGAAAGTT TTGCGAATCA AAGGCTATGT CTTTAAAGAG 
CTGAATAGTT TAAACCTCTT CCCATTACGT AATCTTCCCA GTCTTGAAGT TCTTGATCTT 
GGCACTAACT TTATTAAAAT TGCTGACCTC AGCATATTTA AACAATTTAA AACATTGAAA 
TTCATTGATC TTTCAGTGAA TAAAATATCA CCTTCGGGAG ATTCAACTGA AGGTGGTTTC 
TGCTCTAACA TGAGAACTTC TGTAGAAGGC CATGGGCCCC AGGTCCTTGA AGCATTGCAT 
TATTTCAGAT ATGATGAGTA TGCAAGGAGT TGCCGGTCCA AGAGCAAAGA GCCTCCTTCT 
TTCTTACCTC TTAATGAAGA TTGTTATATG TATGGGCAGA CCTTGGACCT TAGTAGAAAT 
AATATATTTT TTATCAAGTC TTCTGATTTC CAGCATCTTT CTTTCCTCAA ATGCCTAAAC 
TTATCAGGAA ATAGCATTAG CCAGACGCTT AATGGAAGTG AATTTCAGCC TTTAGTGGAG 
TTGAAATATT TGGACTTCTC TAACAATCGG CTTGATTTAC TCTACTCAAC GGCATTTGAG 
GAGCTGCACA ACCTGGAAGT CCTAGATATA AGTAGTAACA GCCATTATTT TCAATCAGAA 
GGAATTACTC ACATGCTAAA TTTTACCAAG AACCTCAAGG TTCTGAAGAA ACTGATGATG 
AACTATAATG ACATCGCTAC CTCCACCAGC AGGACCATGG AGAGTGAATC TCTTCAAATC 
CTGGAGTTCA GAGGCAACCA TTTGGATATT TTATGGAGAG ATGGTGATAA CAGATACTTA 
AAATTCTTTA AGAATCTGCT AAACTTAGAA GAGCTAGACA TCTCTGAAAA TTCTCTGAGT 
TTCTTACCTT TGGGAGTTTT TGATAGTATG CCTCCAAATC TAAAGACTCT CTCCTTGGCC 
AAAAATGGGC TCAAGTCTTT CAGTTGGAAA GGACTACAGA GTCTGAAGAA TCTAGAAACT 
TTGGACCTCA GCTTCAACCA GCTGAAGACT GTCCCTGAGA GATTATCCAA CTGTTCCCGC 
AGCCTCAAGA AACTCATACT TAAGAATAAT CAAATCAGGT GCCTGACAAA GTATTTTCTC 
CAAGGTGCTT TCCAGTTGCG ACATCTGGAC CTCAGCTCAA ATAAAATTCA GGTTATCCAA 
AAGACGAGTT TTCCAGAAAA TGTCCTCAAC AATCTGAACA TTTTGTTTTT GCATCACAAT 
CGATTTCTGT GCAACTGTGA TGCTGTGTGG TTTGTCTGGT GGGTTAACCA TACCGAGGTG 
ACTATTCCTT ACTTGGCCAC AGATGTGACT TGCATGGGAC CAGGAGCACA CAAGGGCCAG 
AGTGTGGTCT CTCTGGATCT ATATACCTGT GAGTTAGATT TGACTAACTT CATCCTGTTC 
TCACTTTCCA TATCAGCAGT TCTCTCTCTG ATGATGATCA CAACAGCAAA CCATCTCTAT 
TTCTGGGATG TGTGGTATAG TTATCATTTC TGTAAAGCCA AAATAAAAGG GTATCGACGT 
CTGATATCAC CCAATTCTTG CTATGATGCT TTCATTGTAT ATGACACTAA AGACCCAGCA 
GTGACAGAGT GGGTTTTGGA TGAGCTGGTG GCCAAATTGG AAGACCCAAG AGAGAAGTGT 
TTTAATTTAT GTCTTGAGGA AAGAGACTGG TTACCAGGGC AGCCTGTTCT GGAAAATCTT 
TCCCAGAGCA TACAGCTTAG CAAAAAGACA GTGTTTGTGA TGACAGACAA GTACGCAAAG 
ACTGAGAATT TTAAGATAGC ATTTTACTTA TCCCATCAGA GGCTCATGGA TGAAAAAGTG 
GATGTAATCA TCTTGATATT CCTTGAGAAG CCCCTTCAGA AGTCCAAGTT TCTCCAACTC 
CGGAAGAGGC TCTGTGGCAG TTCTGTCCTT GAGTGGCCAA CAAACCCACA GGCTCACCCG 
TACTTCTGGC AGTGTCTGAA AAATGCCCTG GCCACAGACA ATCACGTGAC CTACAGTCAG 
GTGTTCAAAG AGACAGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The double-stranded RNA bluetongue virus induces type I interferon in plasmacytoid dendritic cells via a MYD88-dependent TLR7/8-independent signaling pathway.
Journal of virology86(10)5817-28(2012 May)
Ruscanu S,Pascale F,Bourge M,Hemati B,Elhmouzi-Younes J,Urien C,Bonneau M,Takamatsu H,Hope J,Mertens P,Meyer G,Stewart M,Roy P,Meurs EF,Dabo S,Zientara S,Breard E,Sailleau C,Chauveau E,Vitour D,Charley B,Schwartz-Cornil I

Characterization of ovine TLR7 and TLR8 protein coding regions, detection of mutations and Maedi Visna virus infection.
Veterinary immunology and immunopathology138(1-2)51-9(2010 Nov)
Mikula I,Bhide M,Pastorekova S,Mikula I

Molecular cloning and characterization of Toll-like receptors 1-10 in sheep.
Veterinary immunology and immunopathology127(1-2)94-105(2009 Jan)
Chang JS,Russell GC,Jann O,Glass EJ,Werling D,Haig DM

Differential expression of pattern recognition receptors in sheep tissues and leukocyte subsets.
Veterinary immunology and immunopathology118(3-4)252-62(2007 Aug)
Nalubamba KS,Gossner AG,Dalziel RG,Hopkins J

Identification and expression of Toll-like receptors 1-10 in selected bovine and ovine tissues.
Veterinary immunology and immunopathology109(1-2)23-30(2006 Jan)
Menzies M,Ingham A