IL1B cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	IL1B
Entrez Gene ID	443539
Full Name	interleukin 1 beta
Synonyms	IL-1B
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001009465.2	NP_001009465.2	interleukin-1 beta precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Unprenylated RhoA contributes to IL-1? hypersecretion in mevalonate kinase deficiency model through stimulation of Rac1 activity.
van der Burgh R, Pervolaraki K, Turkenburg M, Waterham HR, Frenkel J, Boes M
The Journal of biological chemistry289(40)27757-65(2014 Oct)

Can proinflammatory cytokine gene expression explain multifidus muscle fiber changes after an intervertebral disc lesion?
Hodges PW, James G, Blomster L, Hall L, Schmid AB, Shu C, Little C, Melrose J
Spine39(13)1010-7(2014 Jun)

Production and expression of RANTES (CCL5) by human disc cells and modulation by IL-1-? and TNF-? in 3D culture.
Gruber HE, Hoelscher GL, Ingram JA, Bethea S, Norton HJ, Hanley EN Jr
Experimental and molecular pathology96(2)133-8(2014 Apr)

Differential association of RANTES-403 and IL-1B-1464 polymorphisms on histological subtypes in male Korean patients with gastric cancer.
Kim J, Kim JW, Kim Y, Lee KA
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine35(4)3765-70(2014 Apr)

Peripheral injection of SB203580 inhibits the inflammatory-dependent synthesis of proinflammatory cytokines in the hypothalamus.
Herman AP, Krawczy?ska A, Bochenek J, Antushevich H, Herman A, Tomaszewska-Zaremba D
BioMed research international2014475152(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Inactivation of RhoA due to lack of prenylation leads to Rac1 activation and subsequent priming for IL-1beta secretion.
Title: Unprenylated RhoA contributes to IL-1? hypersecretion in mevalonate kinase deficiency model through stimulation of Rac1 activity.

Interleukin-1beta was increased only on the injured side of multifidus in disc disease.
Title: Can proinflammatory cytokine gene expression explain multifidus muscle fiber changes after an intervertebral disc lesion?

In vitro studies with IL-1-beta and TNF-alpha challenges, both proinflammatory cytokines resulted in elevated levels of CCL5 in conditioned media (p<0.01)
Title: Production and expression of RANTES (CCL5) by human disc cells and modulation by IL-1-? and TNF-? in 3D culture.

RANTES-403 was significantly associated with the risk of developing diffuse-type gastric cancer in men and found a possible gene-gene interaction between RANTES and IL-1B polymorphisms in gastric cancer carcinogenesis.
Title: Differential association of RANTES-403 and IL-1B-1464 polymorphisms on histological subtypes in male Korean patients with gastric cancer.

Intravenous injection of SB203580 successfully inhibited synthesis of IL-1beta and reduced the production of IL-6 in the hypothalamus.
Title: Peripheral injection of SB203580 inhibits the inflammatory-dependent synthesis of proinflammatory cytokines in the hypothalamus.

The following IL1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSm00061	NM_001009465.2 Latest version!	Ovis aries interleukin 1 beta (IL1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IL1B ( NM_001009465.2 ) cDNA ORF clone, Ovis aries(sheep) -> NP_001009465.2 Ovis aries interleukin 1 beta (IL1B), mRNA.

Price & Availability↑

CloneID	OSm00061
Clone ID Related Accession (Same CDS sequence)	NM_001009465.2
Accession Version	NM_001009465.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Ovis aries(sheep)
Product	interleukin-1 beta precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X54796.1. On Jan 12, 2005 this sequence version replaced NM_001009465.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X54796.1, DY502470.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA104495048, SAMEA104495052 [ECO:0000348] ##Evidence-Data-END##

ATGGCAACCG TACCTGAACC CATCAATGAA GTGATGGCTT GCTACAGTGA TGAGAATGAG 
CTGTTATTTG AGGTTGATGG CCCTAAACAG ATGAAGAGCT GCACCCAACA CCTGGACCTC 
GGCTCCATGG GAGATGGAAA CATCCAGCTG CAGATTTCTC ACAAGCTCTA CAACAAAAGC 
TTCAGGCAGG CAGTGTCGGT CATCGTGGCC ATGGAGAAGC TGAGGAGCCG TGCCTACGAA 
CATGTCTTCC GTGATGATGA CCTGAGGAGC ATCCTTTCAT TCATCTTCGA AGAAGAGCCT 
GTCATCTTCG AAACATCCTC CGATGAGCTT CTGTGTGATG CAGCCGTGCA GTCAGTAAAA 
TGCAAACTCC AGGACAGAGA GCAAAAATCC CTGGTGCTGG ATAGCCCATG TGTGCTGAAG 
GCTCTCCACC TCCTCTCACA GGAAATGAGC CGAGAAGTGG TGTTCTGCAT GAGCTTCGTA 
CAAGGAGAGG AAAGAGACAA CAAGATTCCT GTGGCCTTGG GTATCAGGGA CAAGAATCTA 
TACCTGTCTT GTGTGAAAAA AGGTGATACA CCGACCCTGC AGCTGGAGGA AGTAGACCCC 
AAAGTCTACC CCAAGAGGAA TATGGAAAAG CGATTCGTCT TCTACAAGAC AGAAATCAAG 
AACACAGTTG AATTTGAGTC TGTCCTGTAC CCTAACTGGT ACATCAGCAC TTCTCAAATC 
GAAGAAAAGC CCGTCTTCCT GGGACGTTTT AGAGGTGGCC AGGATATAAC TGACTTCAGA 
ATGGAAACCC TCTCTCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009465.2
CDS	33..833
Translation	MATVPEPINEVMACYSDENELLFEVDGPKQMKSCTQHLDLGSMGDGNIQLQISHKLYNKSFRQAVSVIVAMEKLRSRAYEHVFRDDDLRSILSFIFEEEPVIFETSSDELLCDAAVQSVKCKLQDREQKSLVLDSPCVLKALHLLSQEMSREVVFCMSFVQGEERDNKIPVALGIRDKNLYLSCVKKGDTPTLQLEEVDPKVYPKRNMEKRFVFYKTEIKNTVEFESVLYPNWYISTSQIEEKPVFLGRFRGGQDITDFRMETLSP

Target ORF information:

RefSeq Version	NM_001009465.2
Organism	Ovis aries(sheep)
Definition	Ovis aries interleukin 1 beta (IL1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009465.2

ORF Insert Sequence:

ATGGCAACCG TACCTGAACC CATCAATGAA GTGATGGCTT GCTACAGTGA TGAGAATGAG 
CTGTTATTTG AGGTTGATGG CCCTAAACAG ATGAAGAGCT GCACCCAACA CCTGGACCTC 
GGCTCCATGG GAGATGGAAA CATCCAGCTG CAGATTTCTC ACAAGCTCTA CAACAAAAGC 
TTCAGGCAGG CAGTGTCGGT CATCGTGGCC ATGGAGAAGC TGAGGAGCCG TGCCTACGAA 
CATGTCTTCC GTGATGATGA CCTGAGGAGC ATCCTTTCAT TCATCTTCGA AGAAGAGCCT 
GTCATCTTCG AAACATCCTC CGATGAGCTT CTGTGTGATG CAGCCGTGCA GTCAGTAAAA 
TGCAAACTCC AGGACAGAGA GCAAAAATCC CTGGTGCTGG ATAGCCCATG TGTGCTGAAG 
GCTCTCCACC TCCTCTCACA GGAAATGAGC CGAGAAGTGG TGTTCTGCAT GAGCTTCGTA 
CAAGGAGAGG AAAGAGACAA CAAGATTCCT GTGGCCTTGG GTATCAGGGA CAAGAATCTA 
TACCTGTCTT GTGTGAAAAA AGGTGATACA CCGACCCTGC AGCTGGAGGA AGTAGACCCC 
AAAGTCTACC CCAAGAGGAA TATGGAAAAG CGATTCGTCT TCTACAAGAC AGAAATCAAG 
AACACAGTTG AATTTGAGTC TGTCCTGTAC CCTAACTGGT ACATCAGCAC TTCTCAAATC 
GAAGAAAAGC CCGTCTTCCT GGGACGTTTT AGAGGTGGCC AGGATATAAC TGACTTCAGA 
ATGGAAACCC TCTCTCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Unprenylated RhoA contributes to IL-1? hypersecretion in mevalonate kinase deficiency model through stimulation of Rac1 activity.
The Journal of biological chemistry289(40)27757-65(2014 Oct)
van der Burgh R,Pervolaraki K,Turkenburg M,Waterham HR,Frenkel J,Boes M

Can proinflammatory cytokine gene expression explain multifidus muscle fiber changes after an intervertebral disc lesion?
Spine39(13)1010-7(2014 Jun)
Hodges PW,James G,Blomster L,Hall L,Schmid AB,Shu C,Little C,Melrose J

Production and expression of RANTES (CCL5) by human disc cells and modulation by IL-1-? and TNF-? in 3D culture.
Experimental and molecular pathology96(2)133-8(2014 Apr)
Gruber HE,Hoelscher GL,Ingram JA,Bethea S,Norton HJ,Hanley EN Jr

Differential association of RANTES-403 and IL-1B-1464 polymorphisms on histological subtypes in male Korean patients with gastric cancer.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine35(4)3765-70(2014 Apr)
Kim J,Kim JW,Kim Y,Lee KA

Peripheral injection of SB203580 inhibits the inflammatory-dependent synthesis of proinflammatory cytokines in the hypothalamus.
BioMed research international2014475152(2014)
Herman AP,Krawczy?ska A,Bochenek J,Antushevich H,Herman A,Tomaszewska-Zaremba D