PRL cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRL
Entrez Gene ID	443317
Full Name	prolactin
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001009306.1	NP_001009306.1	prolactin precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Distinct roles of prolactin, epidermal growth factor, and glucocorticoids in ?-casein secretion pathway in lactating mammary epithelial cells.
Kobayashi K, Oyama S, Kuki C, Tsugami Y, Matsunaga K, Suzuki T, Nishimura T
Molecular and cellular endocrinology44016-24(2017 Jan)

Prolactin induces apoptosis of lactotropes in female rodents.
Ferraris J, Z?rate S, Jaita G, Boutillon F, Bernadet M, Auffret J, Seilicovich A, Binart N, Goffin V, Pisera D
PloS one9(5)e97383(2014)

Prolactin and epidermal growth factor stimulate adipophilin synthesis in HC11 mouse mammary epithelial cells via the PI3-kinase/Akt/mTOR pathway.
Pauloin A, Chanat E
Biochimica et biophysica acta1823(5)987-96(2012 May)

Differential sensitivity of specific neuronal populations of the rat hypothalamus to prolactin action.
Sapsford TJ, Kokay IC, Ostberg L, Bridges RS, Grattan DR
The Journal of comparative neurology520(5)1062-77(2012 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicate that PRL, EGF and glucocorticoids have distinct roles in the establishment of beta-casein secretion pathway.
Title: Distinct roles of prolactin, epidermal growth factor, and glucocorticoids in ?-casein secretion pathway in lactating mammary epithelial cells.

Prolactin induces apoptosis of lactotropes
Title: Prolactin induces apoptosis of lactotropes in female rodents.

Prolactin and epidermal growth factor stimulate adipophilin synthesis in HC11 mouse mammary epithelial cells via the PI3-kinase/Akt/mTOR pathway.
Title: Prolactin and epidermal growth factor stimulate adipophilin synthesis in HC11 mouse mammary epithelial cells via the PI3-kinase/Akt/mTOR pathway.

Differences in accessibility of the rat arcuate nucleus to sheep prolactin, together with intrinsic differences in neuroendocrine dopaminergic neurons, facilitate homeostatic feedback regulation of sheep prolactin release in the rat hypothalamus
Title: Differential sensitivity of specific neuronal populations of the rat hypothalamus to prolactin action.

conclude that PRL is an important part of the regulation of the pituitary-gonadal system in moderately seasonal DLS rams; it seems to impair release of LH from gonadotrophs, help maintain functional integrity of Leydig cells, & promote testicular growth
Title: Prolactin regulation of testosterone secretion and testes growth in DLS rams at the onset of seasonal testicular recrudescence.

The following PRL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSm00175	NM_001009306.1 Latest version!	Ovis aries prolactin (PRL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRL ( NM_001009306.1 ) cDNA ORF clone, Ovis aries(sheep) -> NP_001009306.1 Ovis aries prolactin (PRL), mRNA.

Price & Availability↑

CloneID	OSm00175
Clone ID Related Accession (Same CDS sequence)	NM_001009306.1
Accession Version	NM_001009306.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Ovis aries(sheep)
Product	prolactin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M27057.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M27057.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA104495017, SAMEA104495023 [ECO:0000348] ##Evidence-Data-END##

ATGGTGGTGA TGATTTCAAG AAACACACTT CACCAGGACA GCAAAGGTTC AGCGCAGAAA 
GGGTCCCGCC TGCTCCTGCT GCTGGTGGTG TCAAATCTAC TCTTGTGTCA GGGTGTGGTC 
TCCACCCCTG TCTGTCCCAA TGGGCCTGGC GACTGTCAGG TGTCCCTTCG AGACCTGTTT 
GACCGGGCAG TCATGGTGTC CCACTACATT CATAACCTCT CCTCGGAAAT GTTCAATGAA 
TTTGATAAAA GGTATGCCCA GGGCAAAGGG TTCATTACCA TGGCCCTCAA CAGCTGCCAC 
ACCTCCTCCC TTCCTACCCC TGAAGACAAA GAACAAGCCC AACAGACCCA CCATGAAGTC 
CTTATGAGCT TGATTCTTGG GTTGCTGCGC TCCTGGAATG ACCCTCTGTA TCACCTAGTC 
ACAGAGGTGC GGGGTATGAA AGGAGTCCCA GATGCTATTC TATCGAGGGC TATAGAGATT 
GAGGAAGAAA ACAAACGACT TCTGGAAGGC ATGGAGATGA TATTTGGCCA AGTTATTCCT 
GGAGCCAAAG AGACTGAGCC CTACCCTGTG TGGTCAGGAC TCCCATCCCT GCAAACTAAG 
GATGAAGATG CACGTCATTC TGCTTTTTAC AACCTGCTTC ACTGCCTGCG CAGGGATTCA 
AGCAAGATTG ACACTTACCT TAAGCTCCTG AATTGCAGAA TCATCTACAA CAACAACTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009306.1
CDS	61..783
Translation	MVVMISRNTLHQDSKGSAQKGSRLLLLLVVSNLLLCQGVVSTPVCPNGPGDCQVSLRDLFDRAVMVSHYIHNLSSEMFNEFDKRYAQGKGFITMALNSCHTSSLPTPEDKEQAQQTHHEVLMSLILGLLRSWNDPLYHLVTEVRGMKGVPDAILSRAIEIEEENKRLLEGMEMIFGQVIPGAKETEPYPVWSGLPSLQTKDEDARHSAFYNLLHCLRRDSSKIDTYLKLLNCRIIYNNNC

Target ORF information:

RefSeq Version	NM_001009306.1
Organism	Ovis aries(sheep)
Definition	Ovis aries prolactin (PRL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009306.1

ORF Insert Sequence:

ATGGTGGTGA TGATTTCAAG AAACACACTT CACCAGGACA GCAAAGGTTC AGCGCAGAAA 
GGGTCCCGCC TGCTCCTGCT GCTGGTGGTG TCAAATCTAC TCTTGTGTCA GGGTGTGGTC 
TCCACCCCTG TCTGTCCCAA TGGGCCTGGC GACTGTCAGG TGTCCCTTCG AGACCTGTTT 
GACCGGGCAG TCATGGTGTC CCACTACATT CATAACCTCT CCTCGGAAAT GTTCAATGAA 
TTTGATAAAA GGTATGCCCA GGGCAAAGGG TTCATTACCA TGGCCCTCAA CAGCTGCCAC 
ACCTCCTCCC TTCCTACCCC TGAAGACAAA GAACAAGCCC AACAGACCCA CCATGAAGTC 
CTTATGAGCT TGATTCTTGG GTTGCTGCGC TCCTGGAATG ACCCTCTGTA TCACCTAGTC 
ACAGAGGTGC GGGGTATGAA AGGAGTCCCA GATGCTATTC TATCGAGGGC TATAGAGATT 
GAGGAAGAAA ACAAACGACT TCTGGAAGGC ATGGAGATGA TATTTGGCCA AGTTATTCCT 
GGAGCCAAAG AGACTGAGCC CTACCCTGTG TGGTCAGGAC TCCCATCCCT GCAAACTAAG 
GATGAAGATG CACGTCATTC TGCTTTTTAC AACCTGCTTC ACTGCCTGCG CAGGGATTCA 
AGCAAGATTG ACACTTACCT TAAGCTCCTG AATTGCAGAA TCATCTACAA CAACAACTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Distinct roles of prolactin, epidermal growth factor, and glucocorticoids in ?-casein secretion pathway in lactating mammary epithelial cells.
Molecular and cellular endocrinology44016-24(2017 Jan)
Kobayashi K,Oyama S,Kuki C,Tsugami Y,Matsunaga K,Suzuki T,Nishimura T

Prolactin induces apoptosis of lactotropes in female rodents.
PloS one9(5)e97383(2014)
Ferraris J,Z?rate S,Jaita G,Boutillon F,Bernadet M,Auffret J,Seilicovich A,Binart N,Goffin V,Pisera D

Prolactin and epidermal growth factor stimulate adipophilin synthesis in HC11 mouse mammary epithelial cells via the PI3-kinase/Akt/mTOR pathway.
Biochimica et biophysica acta1823(5)987-96(2012 May)
Pauloin A,Chanat E

Differential sensitivity of specific neuronal populations of the rat hypothalamus to prolactin action.
The Journal of comparative neurology520(5)1062-77(2012 Apr)
Sapsford TJ,Kokay IC,Ostberg L,Bridges RS,Grattan DR