SPG7 cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPG7
Entrez Gene ID	101110441
Full Name	SPG7, paraplegin matrix AAA peptidase subunit
Gene Type	protein-coding
Organism	Ovis aries musimon(Ovis orientalis musimon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_027977759.1	XP_027833560.1	paraplegin
XM_015100166.1	XP_014955652.1	paraplegin isoform X1
XM_012163901.2	XP_012019291.1	paraplegin isoform X2
XM_012163902.2	XP_012019292.1	paraplegin isoform X3

Pathways from BioSystems
Gene Ontology

The following SPG7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPG7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSm63910	XM_027977759.1 Latest version!	Ovis aries SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OOv34839	XM_012163901.2 Latest version!	Ovis aries musimon spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OOv34840	XM_012163902.2 Latest version!	Ovis aries musimon spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OSm52558	XM_015100166.1 Latest version!	Ovis aries spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPG7 ( XM_027977759.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_027833560.1 Ovis aries SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSm63910
Clone ID Related Accession (Same CDS sequence)	XM_027977759.1
Accession Version	XM_027977759.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	paraplegin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040265.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CGCGGCTGCT GCTGCGCGCT CTCCGCCCGG GGCCGGGACC GGGGCCGGGG 
AGGCTGCGGT GCCCCTACTC GGGCTGGAAC CCAGGCTGCT CCTCCGGGGC CAAGTGGAGG 
TGGCCGTATG TCGTCCACAG GCCTCCGATG GGCCTCGTCG GGCCTGGTGG CCGAGCCCTG 
CAGAGTTTCC AGTTGCGACT GCTAACCCCT ACCTTTGAAG GAATCAGCAG ATTGTTGGTG 
AAACAGCACA TTGTACAGAA TCCAGTCGGA CTCTGGAAAC TTCTAGGAGG CACTTACTAT 
TTTAACACTT CGAGGAGGAA GCAGAAGACC AAGGACAATG ATAAAGCCAA GGGGAGGAGC 
CCTGAGGATG ACGAAGAGGA GCGCAGGCGC CGGGAGCGCG AGGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCCTGTTCGT CATCGCAGTG ATCATGAGTC TGCTCAATGC GCTGGGCTCC 
AGCGGGGGAA ACATCTCCTG GAATGACTTC GTGCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGGGTGC AAGTGGTGCC TGAGAGCGAC GTGGTGGAGG TGTACCTGCA CCCCGGTGCC 
GTGGTGTTCG GGCGGCCTCG GCTGGCATTG ATGTACCGAA TGCAGGTGGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCAGCTGAG GATGAGCTGA ATATTGAAGG CAAGGACAGG 
ATTCCAGTTT CCTACAAGCG GACGGGATTC TTTGGAAATG CTCTCTACGC CCTGGGGATG 
ACAGCCGTGG GCTTGGCCAT CCTGTGGTAC GTCTTCCGTC TGGCTGGGAT GACAGGACGG 
GAAGGCGGAT TCAGTGCCTT TAATCAGCTG AAAATGGCTC GTTTCACGAT TGTGGATGGC 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAT GTGGCAGGAA TGCATGAGGC CAAACTGGAG 
GTCAAGGAGT TCGTGGATTA TCTGAAGAGC CCGGAGCGCT TCCTTCAGCT TGGCGCCAAG 
GTCCCGAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGCG GGAAGACGCT ACTGGCCAAA 
GCAGTGGCCA CGGAGGCCCA GGTGCCCTTC CTGGCGATGG CTGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGTGC TGCCCGTGTG CGGAGCCTCT TCAAGGAAGC CCGGGCCCGG 
GCCCCCTGCA TTGTGTACAT TGACGAGATC GACGCCGTGG GCAAGAAGCG TTCCACCGCC 
GTGTCCGGTT TCTCCAACAC AGAGGAGGAG CAGACGCTCA ACCAGCTGCT GGTGGAGATG 
GACGGAATGG GTACCACCGA CCACGTCATC GTCCTGGCGG CCACCAACCG AGCTGACATT 
TTGGACAACG CTCTGCTGAG GCCAGGCCGA CTGGACAGGC ATGTTTTCAT CGACCTTCCC 
ACTCTGCAGG AAAGGAAGGA GATCTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACTCGG 
GCCAGCAGCT TTTACTCCCA GCGGCTGGCG GAGCTGACAC CTGGCTTCAG TGGCGCTGAC 
ATTGCCAACA TCTGTAATGA GGCTGCGTTG CACGCAGCGC GGGAGGGGCA CACAGCTGTA 
CACACGTCCA ACCTGGACTA TGCCGTGGAG CGCGTCGTGG CCGGGACTGC CAAGAAGAGC 
AAAGTCCTTT CCAAGGAAGA GCAGAAGGTG GTTGCTTTCC ATGAGTCGGG CCACGCCCTG 
GTGGGCTGGC TGCTGGAGCA CACGGAGGCT GTGATGAAGG TGTCCATCAC TCCTCGGACC 
AACGCAGCCC TGGGCTTTGC TCAGATGCTC CCGAGAGACC AGCACCTCTT CACCAGGGAG 
CAGCTATTTG AGCGCATGTG CATGGCCCTG GGCGGCCGCG CATCCGAGTC CATCTCTTTC 
AACAGGGTCA CTTCCGGGGC ACAGGACGAC CTGCGGAAGG TCACCCGCAT CGCCTACTCC 
ATGGTGAAGC AGTTTGGGAT GGCGCCCAGC ATTGGGCCCG TCTCCTTCCC CGAGGCCCAG 
GAGGGCGTCA CGGGCATCGG ACGGCGTCCA TTCAGCCAGG GCCTCCAGCA GATGATGGAC 
CACGAAGCGA GGCTGCTGGT GGCCACTGCC TATAGGCATA CGGAACAGGT GCTTCGGGAC 
AACCTGGACA AGCTCCACGC GCTGGCGAGT GCCCTGCTGG AAAAGGAAGT GATCAACTAC 
GAGGACATCG AGGCCCTCAT CGGCCCACCG CCCCACGGGC CCAAGAAAAG GATCGCGCCG 
CAGAAGTGGA GTGATGCCCA GAGAGAGAAG CAGGACTCGG GGGAGGAGGA GGGGGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027833560.1
CDS	21..2360
Translation	MAAARLLLRALRPGPGPGPGRLRCPYSGWNPGCSSGAKWRWPYVVHRPPMGLVGPGGRALQSFQLRLLTPTFEGISRLLVKQHIVQNPVGLWKLLGGTYYFNTSRRKQKTKDNDKAKGRSPEDDEEERRRREREDQMYRERLRTLFVIAVIMSLLNALGSSGGNISWNDFVHEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIEGKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDGKMGKGVSFKDVAGMHEAKLEVKEFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTAVSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLAATNRADILDNALLRPGRLDRHVFIDLPTLQERKEIFEQHLKSLKLTRASSFYSQRLAELTPGFSGADIANICNEAALHAAREGHTAVHTSNLDYAVERVVAGTAKKSKVLSKEEQKVVAFHESGHALVGWLLEHTEAVMKVSITPRTNAALGFAQMLPRDQHLFTREQLFERMCMALGGRASESISFNRVTSGAQDDLRKVTRIAYSMVKQFGMAPSIGPVSFPEAQEGVTGIGRRPFSQGLQQMMDHEARLLVATAYRHTEQVLRDNLDKLHALASALLEKEVINYEDIEALIGPPPHGPKKRIAPQKWSDAQREKQDSGEEEGA

Target ORF information:

RefSeq Version	XM_027977759.1
Organism	Ovis aries(sheep)
Definition	Ovis aries SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027977759.1

ORF Insert Sequence:

ATGGCGGCCG CGCGGCTGCT GCTGCGCGCT CTCCGCCCGG GGCCGGGACC GGGGCCGGGG 
AGGCTGCGGT GCCCCTACTC GGGCTGGAAC CCAGGCTGCT CCTCCGGGGC CAAGTGGAGG 
TGGCCGTATG TCGTCCACAG GCCTCCGATG GGCCTCGTCG GGCCTGGTGG CCGAGCCCTG 
CAGAGTTTCC AGTTGCGACT GCTAACCCCT ACCTTTGAAG GAATCAGCAG ATTGTTGGTG 
AAACAGCACA TTGTACAGAA TCCAGTCGGA CTCTGGAAAC TTCTAGGAGG CACTTACTAT 
TTTAACACTT CGAGGAGGAA GCAGAAGACC AAGGACAATG ATAAAGCCAA GGGGAGGAGC 
CCTGAGGATG ACGAAGAGGA GCGCAGGCGC CGGGAGCGCG AGGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCCTGTTCGT CATCGCAGTG ATCATGAGTC TGCTCAATGC GCTGGGCTCC 
AGCGGGGGAA ACATCTCCTG GAATGACTTC GTGCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGGGTGC AAGTGGTGCC TGAGAGCGAC GTGGTGGAGG TGTACCTGCA CCCCGGTGCC 
GTGGTGTTCG GGCGGCCTCG GCTGGCATTG ATGTACCGAA TGCAGGTGGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCAGCTGAG GATGAGCTGA ATATTGAAGG CAAGGACAGG 
ATTCCAGTTT CCTACAAGCG GACGGGATTC TTTGGAAATG CTCTCTACGC CCTGGGGATG 
ACAGCCGTGG GCTTGGCCAT CCTGTGGTAC GTCTTCCGTC TGGCTGGGAT GACAGGACGG 
GAAGGCGGAT TCAGTGCCTT TAATCAGCTG AAAATGGCTC GTTTCACGAT TGTGGATGGC 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAT GTGGCAGGAA TGCATGAGGC CAAACTGGAG 
GTCAAGGAGT TCGTGGATTA TCTGAAGAGC CCGGAGCGCT TCCTTCAGCT TGGCGCCAAG 
GTCCCGAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGCG GGAAGACGCT ACTGGCCAAA 
GCAGTGGCCA CGGAGGCCCA GGTGCCCTTC CTGGCGATGG CTGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGTGC TGCCCGTGTG CGGAGCCTCT TCAAGGAAGC CCGGGCCCGG 
GCCCCCTGCA TTGTGTACAT TGACGAGATC GACGCCGTGG GCAAGAAGCG TTCCACCGCC 
GTGTCCGGTT TCTCCAACAC AGAGGAGGAG CAGACGCTCA ACCAGCTGCT GGTGGAGATG 
GACGGAATGG GTACCACCGA CCACGTCATC GTCCTGGCGG CCACCAACCG AGCTGACATT 
TTGGACAACG CTCTGCTGAG GCCAGGCCGA CTGGACAGGC ATGTTTTCAT CGACCTTCCC 
ACTCTGCAGG AAAGGAAGGA GATCTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACTCGG 
GCCAGCAGCT TTTACTCCCA GCGGCTGGCG GAGCTGACAC CTGGCTTCAG TGGCGCTGAC 
ATTGCCAACA TCTGTAATGA GGCTGCGTTG CACGCAGCGC GGGAGGGGCA CACAGCTGTA 
CACACGTCCA ACCTGGACTA TGCCGTGGAG CGCGTCGTGG CCGGGACTGC CAAGAAGAGC 
AAAGTCCTTT CCAAGGAAGA GCAGAAGGTG GTTGCTTTCC ATGAGTCGGG CCACGCCCTG 
GTGGGCTGGC TGCTGGAGCA CACGGAGGCT GTGATGAAGG TGTCCATCAC TCCTCGGACC 
AACGCAGCCC TGGGCTTTGC TCAGATGCTC CCGAGAGACC AGCACCTCTT CACCAGGGAG 
CAGCTATTTG AGCGCATGTG CATGGCCCTG GGCGGCCGCG CATCCGAGTC CATCTCTTTC 
AACAGGGTCA CTTCCGGGGC ACAGGACGAC CTGCGGAAGG TCACCCGCAT CGCCTACTCC 
ATGGTGAAGC AGTTTGGGAT GGCGCCCAGC ATTGGGCCCG TCTCCTTCCC CGAGGCCCAG 
GAGGGCGTCA CGGGCATCGG ACGGCGTCCA TTCAGCCAGG GCCTCCAGCA GATGATGGAC 
CACGAAGCGA GGCTGCTGGT GGCCACTGCC TATAGGCATA CGGAACAGGT GCTTCGGGAC 
AACCTGGACA AGCTCCACGC GCTGGCGAGT GCCCTGCTGG AAAAGGAAGT GATCAACTAC 
GAGGACATCG AGGCCCTCAT CGGCCCACCG CCCCACGGGC CCAAGAAAAG GATCGCGCCG 
CAGAAGTGGA GTGATGCCCA GAGAGAGAAG CAGGACTCGG GGGAGGAGGA GGGGGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG7 ( XM_012163901.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012019291.1 Ovis aries musimon spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv34839
Clone ID Related Accession (Same CDS sequence)	XM_012163901.2
Accession Version	XM_012163901.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	paraplegin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012163901.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CGTGGCTGCT GCTGCGCGCT CTCCGCCCGG GGCCGGGACC GGGGCCGGGA 
CCGGGGCCGG GGAGGCTGCG GTGCCCCTAC TCGGGCTGGA ACCCAGGCTG CTCCTCCGGG 
GCCAAGTGGA GGTGGCCGTA TGTCGTCCAC AGGCCTCCGA TGGGCCTCGT CGGGCCTGGT 
GGCCGAGCCC TGCAGAGTTT CCAGTTGCGA CTGCTAACCC CTACCTTTGA AGGAATCAGC 
AGATTGTTGG TGAAACAGCA CATTGTACAG AATCCAGTCG GACTCTGGAA ACTTCTAGGA 
GGCACTTACT ATTTTAACAC TTCGAGGAGG AAGCAGAAGA CCAAGGACAA TGATAAAGCC 
AAGGGGAGGA GCCCTGAGGA TGACGAAGAG GAGCGCAGGC GCCGGGAGCG CGAGGACCAG 
ATGTACCGAG AGCGGCTGCG CACCCTGTTC GTCATCGCAG TGATCATGAG TCTGCTCAAT 
GCGCTGGGCT CCAGCGGGGG AAACATCTCC TGGAATGACT TCGTGCACGA GATGCTGGCC 
AAGGGCGAGG TGCAGCGGGT GCAAGTGGTG CCTGAGAGCG ACGTGGTGGA GGTGTACCTG 
CACCCCGGTG CCGTGGTGTT CGGGCGGCCT CGGCTGGCAT TGATGTACCG AATGCAGGTG 
GCAAACATCG ACAAATTTGA AGAGAAGCTT CGAGCAGCTG AGGATGAGCT GAATATTGAA 
GGCAAGGACA GGATTCCAGT TTCCTACAAG CGGACGGGAT TCTTTGGAAA TGCTCTCTAC 
GCCCTGGGGA TGACAGCCGT GGGCTTGGCC ATCCTGTGGT ACGTCTTCCG TCTGGCTGGG 
ATGACAGGAC GGGAAGGCGG ATTCAGTGCC TTTAATCAGC TGAAAATGGC TCGTTTCACG 
ATTGTGGATG GCAAGATGGG GAAAGGAGTC AGCTTCAAAG ATGTGGCAGG AATGCATGAG 
GCCAAGCTGG AGGTCAAGGA GTTCGTGGAT TATCTGAAGA GCCCGGAGCG CTTCCTTCAG 
CTTGGCGCCA AGGTCCCGAA GGGCGCACTG CTGCTCGGCC CCCCCGGCTG CGGGAAGACG 
CTACTGGCCA AAGCAGTGGC CACGGAGGCC CAGGTGCCCT TCCTGGCGAT GGCTGGCCCA 
GAGTTCGTGG AGGTCATTGG AGGCCTCGGT GCTGCCCGTG TGCGGAGCCT CTTCAAGGAA 
GCCCGGGCCC GGGCCCCCTG CATTGTGTAC ATTGACGAGA TCGACGCCGT GGGCAAGAAG 
CGTTCCACCG CCGTGTCCGG TTTCTCCAAC ACAGAGGAGG AGCAGACGCT CAACCAGCTG 
CTGGTGGAGA TGGACGGAAT GGGTACCACC GACCACGTCA TCGTCCTGGC GGCCACCAAC 
CGAGCTGACA TTTTGGACAA CGCTCTGCTG AGGCCAGGCC GACTGGACAG GCATGTTTTC 
ATCGACCTTC CCACTCTGCA GGAAAGGAAG GAGATCTTTG AGCAGCACCT GAAGAGCCTG 
AAGCTGACTC GGGCCAGCAG CTTTTACTCC CAGCGGCTGG CGGAGCTGAC ACCTGGCTTC 
AGTGGCGCTG ACATTGCCAA CATCTGTAAT GAGGCTGCAT TGCACGCGGC GCGGGAGGGG 
CACACAGCTG TACACACGTC CAACCTGGAC TATGCCGTGG AGCGCGTCGT GGCCGGGACT 
GCCAAGAAGA GCAAAGTCCT TTCCAAGGAA GAGCAGAAGG TGGTTGCTTT CCATGAGTCG 
GGCCACGCCC TGGTGGGCTG GCTGCTGGAG CACACGGAGG CTGTGATGAA GGTGTCCATC 
ACTCCTCGGA CCAACGCAGC CCTGGGCTTT GCTCAGATGC TCCCGAGAGA CCAGCACCTC 
TTCACCAGGG AGCAGCTATT TGAGCGCATG TGCATGGCCC TGGGTGGCCG CGCATCCGAG 
TCCATCTCTT TCAACAGGGT CACTTCCGGG GCACAGGACG ACCTGCGGAA GGTCACCCGC 
ATCGCCTACT CCATGGTGAA GCAGTTTGGG ATGGCGCCCA GCATTGGGCC CGTCTCCTTC 
CCCGAGGCCC AGGAGGGCGT CACGGGCATC GGACGGCGTC CATTCAGCCA GGGCCTCCAG 
CAGATGATGG ACCACGAAGC GAGGCTGCTG GTGGCCACTG CCTATAGGCA TACGGAACAG 
GTGCTTCGGG ACAACCTGGA CAAGCTCCAC GCGCTGGCGA GTGCCCTGCT GGAAAAGGAA 
GTGATCAACT ACGAGGACAT CGAGGCCCTC ATCGGCCCAC CGCCCCACGG GCCCAAGAAA 
AGGATCGCGC CGCAGAAGTG GAGTGATGCC CAGAGAGAGA AGCAGGACTC GGGGGAGGAG 
GAGGGGGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012019291.1
CDS	1..2352
Translation	MAAAWLLLRALRPGPGPGPGPGPGRLRCPYSGWNPGCSSGAKWRWPYVVHRPPMGLVGPGGRALQSFQLRLLTPTFEGISRLLVKQHIVQNPVGLWKLLGGTYYFNTSRRKQKTKDNDKAKGRSPEDDEEERRRREREDQMYRERLRTLFVIAVIMSLLNALGSSGGNISWNDFVHEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIEGKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDGKMGKGVSFKDVAGMHEAKLEVKEFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTAVSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLAATNRADILDNALLRPGRLDRHVFIDLPTLQERKEIFEQHLKSLKLTRASSFYSQRLAELTPGFSGADIANICNEAALHAAREGHTAVHTSNLDYAVERVVAGTAKKSKVLSKEEQKVVAFHESGHALVGWLLEHTEAVMKVSITPRTNAALGFAQMLPRDQHLFTREQLFERMCMALGGRASESISFNRVTSGAQDDLRKVTRIAYSMVKQFGMAPSIGPVSFPEAQEGVTGIGRRPFSQGLQQMMDHEARLLVATAYRHTEQVLRDNLDKLHALASALLEKEVINYEDIEALIGPPPHGPKKRIAPQKWSDAQREKQDSGEEEGA

Target ORF information:

RefSeq Version	XM_012163901.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012163901.2

ORF Insert Sequence:

ATGGCGGCCG CGTGGCTGCT GCTGCGCGCT CTCCGCCCGG GGCCGGGACC GGGGCCGGGA 
CCGGGGCCGG GGAGGCTGCG GTGCCCCTAC TCGGGCTGGA ACCCAGGCTG CTCCTCCGGG 
GCCAAGTGGA GGTGGCCGTA TGTCGTCCAC AGGCCTCCGA TGGGCCTCGT CGGGCCTGGT 
GGCCGAGCCC TGCAGAGTTT CCAGTTGCGA CTGCTAACCC CTACCTTTGA AGGAATCAGC 
AGATTGTTGG TGAAACAGCA CATTGTACAG AATCCAGTCG GACTCTGGAA ACTTCTAGGA 
GGCACTTACT ATTTTAACAC TTCGAGGAGG AAGCAGAAGA CCAAGGACAA TGATAAAGCC 
AAGGGGAGGA GCCCTGAGGA TGACGAAGAG GAGCGCAGGC GCCGGGAGCG CGAGGACCAG 
ATGTACCGAG AGCGGCTGCG CACCCTGTTC GTCATCGCAG TGATCATGAG TCTGCTCAAT 
GCGCTGGGCT CCAGCGGGGG AAACATCTCC TGGAATGACT TCGTGCACGA GATGCTGGCC 
AAGGGCGAGG TGCAGCGGGT GCAAGTGGTG CCTGAGAGCG ACGTGGTGGA GGTGTACCTG 
CACCCCGGTG CCGTGGTGTT CGGGCGGCCT CGGCTGGCAT TGATGTACCG AATGCAGGTG 
GCAAACATCG ACAAATTTGA AGAGAAGCTT CGAGCAGCTG AGGATGAGCT GAATATTGAA 
GGCAAGGACA GGATTCCAGT TTCCTACAAG CGGACGGGAT TCTTTGGAAA TGCTCTCTAC 
GCCCTGGGGA TGACAGCCGT GGGCTTGGCC ATCCTGTGGT ACGTCTTCCG TCTGGCTGGG 
ATGACAGGAC GGGAAGGCGG ATTCAGTGCC TTTAATCAGC TGAAAATGGC TCGTTTCACG 
ATTGTGGATG GCAAGATGGG GAAAGGAGTC AGCTTCAAAG ATGTGGCAGG AATGCATGAG 
GCCAAGCTGG AGGTCAAGGA GTTCGTGGAT TATCTGAAGA GCCCGGAGCG CTTCCTTCAG 
CTTGGCGCCA AGGTCCCGAA GGGCGCACTG CTGCTCGGCC CCCCCGGCTG CGGGAAGACG 
CTACTGGCCA AAGCAGTGGC CACGGAGGCC CAGGTGCCCT TCCTGGCGAT GGCTGGCCCA 
GAGTTCGTGG AGGTCATTGG AGGCCTCGGT GCTGCCCGTG TGCGGAGCCT CTTCAAGGAA 
GCCCGGGCCC GGGCCCCCTG CATTGTGTAC ATTGACGAGA TCGACGCCGT GGGCAAGAAG 
CGTTCCACCG CCGTGTCCGG TTTCTCCAAC ACAGAGGAGG AGCAGACGCT CAACCAGCTG 
CTGGTGGAGA TGGACGGAAT GGGTACCACC GACCACGTCA TCGTCCTGGC GGCCACCAAC 
CGAGCTGACA TTTTGGACAA CGCTCTGCTG AGGCCAGGCC GACTGGACAG GCATGTTTTC 
ATCGACCTTC CCACTCTGCA GGAAAGGAAG GAGATCTTTG AGCAGCACCT GAAGAGCCTG 
AAGCTGACTC GGGCCAGCAG CTTTTACTCC CAGCGGCTGG CGGAGCTGAC ACCTGGCTTC 
AGTGGCGCTG ACATTGCCAA CATCTGTAAT GAGGCTGCAT TGCACGCGGC GCGGGAGGGG 
CACACAGCTG TACACACGTC CAACCTGGAC TATGCCGTGG AGCGCGTCGT GGCCGGGACT 
GCCAAGAAGA GCAAAGTCCT TTCCAAGGAA GAGCAGAAGG TGGTTGCTTT CCATGAGTCG 
GGCCACGCCC TGGTGGGCTG GCTGCTGGAG CACACGGAGG CTGTGATGAA GGTGTCCATC 
ACTCCTCGGA CCAACGCAGC CCTGGGCTTT GCTCAGATGC TCCCGAGAGA CCAGCACCTC 
TTCACCAGGG AGCAGCTATT TGAGCGCATG TGCATGGCCC TGGGTGGCCG CGCATCCGAG 
TCCATCTCTT TCAACAGGGT CACTTCCGGG GCACAGGACG ACCTGCGGAA GGTCACCCGC 
ATCGCCTACT CCATGGTGAA GCAGTTTGGG ATGGCGCCCA GCATTGGGCC CGTCTCCTTC 
CCCGAGGCCC AGGAGGGCGT CACGGGCATC GGACGGCGTC CATTCAGCCA GGGCCTCCAG 
CAGATGATGG ACCACGAAGC GAGGCTGCTG GTGGCCACTG CCTATAGGCA TACGGAACAG 
GTGCTTCGGG ACAACCTGGA CAAGCTCCAC GCGCTGGCGA GTGCCCTGCT GGAAAAGGAA 
GTGATCAACT ACGAGGACAT CGAGGCCCTC ATCGGCCCAC CGCCCCACGG GCCCAAGAAA 
AGGATCGCGC CGCAGAAGTG GAGTGATGCC CAGAGAGAGA AGCAGGACTC GGGGGAGGAG 
GAGGGGGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG7 ( XM_012163902.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012019292.1 Ovis aries musimon spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), transcript variant X3, mRNA.

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CloneID	OOv34840
Clone ID Related Accession (Same CDS sequence)	XM_012163902.2
Accession Version	XM_012163902.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	paraplegin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012163902.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CGTGGCTGCT GCTGCGCGCT CTCCGCCCGG GGCCGGGACC GGGGCCGGGA 
CCGGGGCCGG GGAGGCTGCG GTGCCCCTAC TCGGGCTGGA ACCCAGGCTG CTCCTCCGGG 
GCCAAGTGGA GGTGGCCGTA TGTCGTCCAC AGGCCTCCGA TGGGCCTCGT CGGGCCTGGT 
GGCCGAGCCC TGCAGAGTTT CCAGTTGCGA CTGCTAACCC CTACCTTTGA AGGAATCAGC 
AGATTGTTGG TGAAACAGCA CATTGTACAG AATCCAGTCG GACTCTGGAA ACTTCTAGGA 
GGCACTTACT ATTTTAACAC TTCGAGGAGG AAGCAGAAGA CCAAGGACAA TGATAAAGCC 
AAGGGGAGGA GCCCTGAGGA TGACGAAGAG GAGCGCAGGC GCCGGGAGCG CGAGGACCAG 
ATGTACCGAG AGCGGCTGCG CACCCTGTTC GTCATCGCAG TGATCATGAG TCTGCTCAAT 
GCGCTGGGCT CCAGCGGGGG AAACATCTCC TGGAATGACT TCGTGCACGA GATGCTGGCC 
AAGGGCGAGG TGCAGCGGGT GCAAGTGGTG CCTGAGAGCG ACGTGGTGGA GGTGTACCTG 
CACCCCGGTG CCGTGGTGTT CGGGCGGCCT CGGCTGGCAT TGATGTACCG AATGCAGGTG 
GCAAACATCG ACAAATTTGA AGAGAAGCTT CGAGCAGCTG AGGATGAGCT GAATATTGAA 
GGCAAGGACA GGATTCCAGT TTCCTACAAG CGGACGGGAT TCTTTGGAAA TGCTCTCTAC 
GCCCTGGGGA TGACAGCCGT GGGCTTGGCC ATCCTGTGGT ACGTCTTCCG TCTGGCTGGG 
ATGACAGGAC GGGAAGGCGG ATTCAGTGCC TTTAATCAGC TGAAAATGGC TCGTTTCACG 
ATTGTGGATG GCAAGATGGG GAAAGGAGTC AGCTTCAAAG ATGTGGCAGG AATGCATGAG 
GCCAAGCTGG AGGTCAAGGA GTTCGTGGAT TATCTGAAGA GCCCGGAGCG CTTCCTTCAG 
CTTGGCGCCA AGGTCCCGAA GGGCGCACTG CTGCTCGGCC CCCCCGGCTG CGGGAAGACG 
CTACTGGCCA AAGCAGTGGC CACGGAGGCC CAGGTGCCCT TCCTGGCGAT GGCTGGCCCA 
GAGTTCGTGG AGGTCATTGG AGGCCTCGGT GCTGCCCGTG TGCGGAGCCT CTTCAAGGAA 
GCCCGGGCCC GGGCCCCCTG CATTGTGTAC ATTGACGAGA TCGACGCCGT GGGCAAGAAG 
CGTTCCACCG CCGTGTCCGG TTTCTCCAAC ACAGAGGAGG AGCAGACGCT CAACCAGCTG 
CTGGTGGAGA TGGACGGAAT GGGTACCACC GACCACGTCA TCGTCCTGGC GGCCACCAAC 
CGAGCTGACA TTTTGGACAA CGCTCTGCTG AGGCCAGGCC GACTGGACAG GCATGTTTTC 
ATCGACCTTC CCACTCTGCA GGAAAGGAAG GAGATCTTTG AGCAGCACCT GAAGAGCCTG 
AAGCTGACTC GGGCCAGCAG CTTTTACTCC CAGCGGCTGG CGGAGCTGAC ACCTGGCTTC 
AGTGGCGCTG ACATTGCCAA CATCTGTAAT GAGGCTGCAT TGCACGCGGC GCGGGAGGGG 
CACACAGCTG TACACACGTC CAACCTGGAC TATGCCGTGG AGCGCGTCGT GGCCGGGACT 
GCCAAGAAGA GCAAAGTCCT TTCCAAGGAA GAGCAGAAGG TGGTTGCTTT CCATGAGTCG 
GGCCACGCCC TGGTGGGCTG GCTGCTGGAG CACACGGAGG CTGTGATGAA GGTGTCCATC 
ACTCCTCGGA CCAACGCAGC CCTGGGCTTT GCTCAGATGC TCCCGAGAGA CCAGCACCTC 
TTCACCAGGG AGCAGCTATT TGAGCGCATG TGCATGGCCC TGGGTGGCCG CGCATCCGAG 
TCCATCTCTT TCAACAGGGT CACTTCCGGG GCACAGGACG ACCTGCGGAA GGTCACCCGC 
ATCGCCTACT CCATGGTGAA GCAGTTTGGG ATGGCGCCCA GCATTGGGCC CGTCTCCTTC 
CCCGAGGCCC AGGAGGGCGT CACGGGCATC GGACGGCGTC CATTCAGCCA GGGCCTCCAG 
CAGATGATGG ACCACTGCCA GGGCCAGGCC ACAGGGAACC CCGTGCACAG CGGCAGCATC 
CTGACCCATC ACACGTCTTC TCCCTTCGCA GGAAGCGAGG CTGCTGGTGG CCACTGCCTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012019292.1
CDS	17..2239
Translation	MAAAWLLLRALRPGPGPGPGPGPGRLRCPYSGWNPGCSSGAKWRWPYVVHRPPMGLVGPGGRALQSFQLRLLTPTFEGISRLLVKQHIVQNPVGLWKLLGGTYYFNTSRRKQKTKDNDKAKGRSPEDDEEERRRREREDQMYRERLRTLFVIAVIMSLLNALGSSGGNISWNDFVHEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIEGKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDGKMGKGVSFKDVAGMHEAKLEVKEFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTAVSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLAATNRADILDNALLRPGRLDRHVFIDLPTLQERKEIFEQHLKSLKLTRASSFYSQRLAELTPGFSGADIANICNEAALHAAREGHTAVHTSNLDYAVERVVAGTAKKSKVLSKEEQKVVAFHESGHALVGWLLEHTEAVMKVSITPRTNAALGFAQMLPRDQHLFTREQLFERMCMALGGRASESISFNRVTSGAQDDLRKVTRIAYSMVKQFGMAPSIGPVSFPEAQEGVTGIGRRPFSQGLQQMMDHCQGQATGNPVHSGSILTHHTSSPFAGSEAAGGHCL

Target ORF information:

RefSeq Version	XM_012163902.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012163902.2

ORF Insert Sequence:

ATGGCGGCCG CGTGGCTGCT GCTGCGCGCT CTCCGCCCGG GGCCGGGACC GGGGCCGGGA 
CCGGGGCCGG GGAGGCTGCG GTGCCCCTAC TCGGGCTGGA ACCCAGGCTG CTCCTCCGGG 
GCCAAGTGGA GGTGGCCGTA TGTCGTCCAC AGGCCTCCGA TGGGCCTCGT CGGGCCTGGT 
GGCCGAGCCC TGCAGAGTTT CCAGTTGCGA CTGCTAACCC CTACCTTTGA AGGAATCAGC 
AGATTGTTGG TGAAACAGCA CATTGTACAG AATCCAGTCG GACTCTGGAA ACTTCTAGGA 
GGCACTTACT ATTTTAACAC TTCGAGGAGG AAGCAGAAGA CCAAGGACAA TGATAAAGCC 
AAGGGGAGGA GCCCTGAGGA TGACGAAGAG GAGCGCAGGC GCCGGGAGCG CGAGGACCAG 
ATGTACCGAG AGCGGCTGCG CACCCTGTTC GTCATCGCAG TGATCATGAG TCTGCTCAAT 
GCGCTGGGCT CCAGCGGGGG AAACATCTCC TGGAATGACT TCGTGCACGA GATGCTGGCC 
AAGGGCGAGG TGCAGCGGGT GCAAGTGGTG CCTGAGAGCG ACGTGGTGGA GGTGTACCTG 
CACCCCGGTG CCGTGGTGTT CGGGCGGCCT CGGCTGGCAT TGATGTACCG AATGCAGGTG 
GCAAACATCG ACAAATTTGA AGAGAAGCTT CGAGCAGCTG AGGATGAGCT GAATATTGAA 
GGCAAGGACA GGATTCCAGT TTCCTACAAG CGGACGGGAT TCTTTGGAAA TGCTCTCTAC 
GCCCTGGGGA TGACAGCCGT GGGCTTGGCC ATCCTGTGGT ACGTCTTCCG TCTGGCTGGG 
ATGACAGGAC GGGAAGGCGG ATTCAGTGCC TTTAATCAGC TGAAAATGGC TCGTTTCACG 
ATTGTGGATG GCAAGATGGG GAAAGGAGTC AGCTTCAAAG ATGTGGCAGG AATGCATGAG 
GCCAAGCTGG AGGTCAAGGA GTTCGTGGAT TATCTGAAGA GCCCGGAGCG CTTCCTTCAG 
CTTGGCGCCA AGGTCCCGAA GGGCGCACTG CTGCTCGGCC CCCCCGGCTG CGGGAAGACG 
CTACTGGCCA AAGCAGTGGC CACGGAGGCC CAGGTGCCCT TCCTGGCGAT GGCTGGCCCA 
GAGTTCGTGG AGGTCATTGG AGGCCTCGGT GCTGCCCGTG TGCGGAGCCT CTTCAAGGAA 
GCCCGGGCCC GGGCCCCCTG CATTGTGTAC ATTGACGAGA TCGACGCCGT GGGCAAGAAG 
CGTTCCACCG CCGTGTCCGG TTTCTCCAAC ACAGAGGAGG AGCAGACGCT CAACCAGCTG 
CTGGTGGAGA TGGACGGAAT GGGTACCACC GACCACGTCA TCGTCCTGGC GGCCACCAAC 
CGAGCTGACA TTTTGGACAA CGCTCTGCTG AGGCCAGGCC GACTGGACAG GCATGTTTTC 
ATCGACCTTC CCACTCTGCA GGAAAGGAAG GAGATCTTTG AGCAGCACCT GAAGAGCCTG 
AAGCTGACTC GGGCCAGCAG CTTTTACTCC CAGCGGCTGG CGGAGCTGAC ACCTGGCTTC 
AGTGGCGCTG ACATTGCCAA CATCTGTAAT GAGGCTGCAT TGCACGCGGC GCGGGAGGGG 
CACACAGCTG TACACACGTC CAACCTGGAC TATGCCGTGG AGCGCGTCGT GGCCGGGACT 
GCCAAGAAGA GCAAAGTCCT TTCCAAGGAA GAGCAGAAGG TGGTTGCTTT CCATGAGTCG 
GGCCACGCCC TGGTGGGCTG GCTGCTGGAG CACACGGAGG CTGTGATGAA GGTGTCCATC 
ACTCCTCGGA CCAACGCAGC CCTGGGCTTT GCTCAGATGC TCCCGAGAGA CCAGCACCTC 
TTCACCAGGG AGCAGCTATT TGAGCGCATG TGCATGGCCC TGGGTGGCCG CGCATCCGAG 
TCCATCTCTT TCAACAGGGT CACTTCCGGG GCACAGGACG ACCTGCGGAA GGTCACCCGC 
ATCGCCTACT CCATGGTGAA GCAGTTTGGG ATGGCGCCCA GCATTGGGCC CGTCTCCTTC 
CCCGAGGCCC AGGAGGGCGT CACGGGCATC GGACGGCGTC CATTCAGCCA GGGCCTCCAG 
CAGATGATGG ACCACTGCCA GGGCCAGGCC ACAGGGAACC CCGTGCACAG CGGCAGCATC 
CTGACCCATC ACACGTCTTC TCCCTTCGCA GGAAGCGAGG CTGCTGGTGG CCACTGCCTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG7 ( XM_015100166.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014955652.1 Ovis aries spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), transcript variant X1, mRNA.

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CloneID	OSm52558
Clone ID Related Accession (Same CDS sequence)	XM_015100166.1
Accession Version	XM_015100166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	paraplegin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639023.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 8% of CDS bases ##RefSeq-Attributes-END##

ATGACGATTG CCTTTCTAGT GCCGGAAAGC GGCGAAGGGA AACGGTCATC GAGAAGGGTC 
CGTGGGGGAA GGCGCATTAG AAGGCGCGTG TGGCGGGCCG CCGGCTGCGT CGGAGGCCCC 
GACCCGGGGC CCACCAGGCT GCAGTTCTAC TCGCAGCGCT TGTACTCGCA GTACAAACAA 
GGCTTGACAG GCCTCACTGG AAGAGGCCCG ACCGCGCAGG CGCCCCCGGG ACCGGGGCCG 
GGACCGGGGC CGGGGAGGCT GCGGTGCCCC TACTCGGGCT GGAACCCAGG CTGCTCCTCC 
GGGGCCAAGT GGAGGTGGCC GTATGTCGTC CACAGGCCTC CGATGGGCCT CGTCGGGCCT 
GGTGGCCGAG CCCTGCAGAG TTTCCAGTTG CGACTGCTAA CCCCTACCTT TGAAGGAATC 
AGCAGATTGT TGGTGAAACA GCACATTGTA CAGAATCCAG TCGGACTCTG GAAACTTCTA 
GGAGGCACTT ACTATTTTAA CACTTCGAGG AGGAAGCAGA AGACCAAGGA CAATGATAAA 
GCCAAGGGGA GGAGCCCTGA GGATGACGAA GAGGAGCGCA GGCGCCGGGA GCGCGAGGAC 
CAGATGTACC GAGAGCGGCT GCGCACCCTG TTCGTCATCG CAGTGATCAT GAGTCTGCTC 
AATGCGCTGG GCTCCAGCGG GGGAAACATC TCCTGGAATG ACTTCGTGCA CGAGATGCTG 
GCCAAGGGCG AGGTGCAGCG GGTGCAAGTG GTGCCTGAGA GCGACGTGGT GGAGGTGTAC 
CTGCACCCCG GTGCCGTGGT GTTCGGGCGG CCTCGGCTGG CATTGATGTA CCGAATGCAG 
GTGGCAAACA TCGACAAATT TGAAGAGAAG CTTCGAGCAG CTGAGGATGA GCTGAATATT 
GAAGGCAAGG ACAGGATTCC AGTTTCCTAC AAGCGGACGG GATTCTTTGG AAATGCTCTC 
TACGCCCTGG GGATGACAGC CGTGGGCTTG GCCATCCTGT GGTACGTCTT CCGTCTGGCT 
GGGATGACAG GACGGGAAGG CGGATTCAGT GCCTTTAATC AGCTGAAAAT GGCTCGTTTC 
ACGATTGTGG ATGGCAAGAT GGGGAAAGGA GTCAGCTTCA AAGATGTGGC AGGAATGCAT 
GAGGCCAAGC TGGAGGTCAA GGAGTTCGTG GATTATCTGA AGAGCCCGGA GCGCTTCCTT 
CAGCTTGGCG CCAAGGTCCC GAAGGGCGCA CTGCTGCTCG GCCCCCCCGG CTGCGGGAAG 
ACGCTACTGG CCAAAGCAGT GGCCACGGAG GCCCAGGTGC CCTTCCTGGC GATGGCTGGC 
CCAGAGTTCG TGGAGGTCAT TGGAGGCCTC GGTGCCGCCC GTGTGCGGAG CCTCTTCAAG 
GAAGCCCGGG CCCGGGCCCC CTGCATTGTG TACATTGACG AGATCGACGC CGTGGGCAAG 
AAGCGTTCCA CCGCCGTGTC CGGTTTCTCC AACACAGAGG AGGAGCAGAC GCTCAACCAG 
CTGCTGGTGG AGATGGACGG AATGGGTACC ACCGACCACG TCATCGTCCT GGCGGCCACC 
AACCGAGCTG ACATTTTGGA CAACGCTCTG CTGAGGCCAG GCCGACTGGA CAGGCATGTT 
TTCATCGACC TTCCCACTCT GCAGGAAAGG AAGGAGATCT TTGAGCAGCA CCTGAAGAGC 
CTGAAGCTGA CTCGGGCCAG CAGCTTTTAC TCCCAGCCCC TGGCAACCCC CACCCCTGTG 
CTGACAGGCG CTGACATTGC CAACATCTGT AATGAGGCTG CGTTGCACGC GGCGCGGGAG 
GGGCACACAG CTGTACACAC GTCCAACCTG GACTATGCCG TGGAGCGCGT CGTGGCCGGG 
ACTGCCAAGA AGAGCAAAGT CCTTTCCAAG GAAGAGCAGA AGGTGGTTGC TTTCCATGAG 
TCGGGCCACG CCCTGGTGGG CTGGCTGCTG GAGCACACGG AGGCTGTGAT GAAGGTGTCC 
ATCACTCCTA GGACCAACGC AGCCCTGGGC TTTGCTCAGA TGCTCCCGAG AGACCAGCAC 
CTCTTCACCA GGGAGCAGCT ATTTGAGCGC ATGTGCATGG CCCTGGGCGG CCGCGCATCC 
GAGTCCATCT CTTTCAACAG GGTCACTTCC GGGGCACAGG ACGACCTGCG GAAGGTCACC 
CGCATCGCCT ACTCCATGGT GAAGCAGTTT GGGATGGCGC CCAGCATTGG GCCCGTCTCC 
TTCCCCGAGG CCCAGGAGGG CGTCACGGGC ATCGGACGGC GTCCATTCAG CCAGGGCCTC 
CAGCAGATGA TGGACCACGA AGCGAGGCTG CTGGTGGCCA CTGCCTATAG GCATACGGAA 
CAGGTGCTTC GGGACAACCT GGACAAGCTC CACGCGCTGG CGAGTGCCCT GCTGGAAAAG 
GAAGTGATCA ACTACGAGGA CATCGAGGCC CTCATCGGCC CACCGCCCCA CGGGCCCAAG 
AAAAGGATCG CGCCGCAGAA GTGGAGTGAT GCCCAGAGAG AGAAGCAGGA CTCGGGGGAG 
GAGGAGGGGG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014955652.1
CDS	1..2535
Translation	MTIAFLVPESGEGKRSSRRVRGGRRIRRRVWRAAGCVGGPDPGPTRLQFYSQRLYSQYKQGLTGLTGRGPTAQAPPGPGPGPGPGRLRCPYSGWNPGCSSGAKWRWPYVVHRPPMGLVGPGGRALQSFQLRLLTPTFEGISRLLVKQHIVQNPVGLWKLLGGTYYFNTSRRKQKTKDNDKAKGRSPEDDEEERRRREREDQMYRERLRTLFVIAVIMSLLNALGSSGGNISWNDFVHEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIEGKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDGKMGKGVSFKDVAGMHEAKLEVKEFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTAVSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLAATNRADILDNALLRPGRLDRHVFIDLPTLQERKEIFEQHLKSLKLTRASSFYSQPLATPTPVLTGADIANICNEAALHAAREGHTAVHTSNLDYAVERVVAGTAKKSKVLSKEEQKVVAFHESGHALVGWLLEHTEAVMKVSITPRTNAALGFAQMLPRDQHLFTREQLFERMCMALGGRASESISFNRVTSGAQDDLRKVTRIAYSMVKQFGMAPSIGPVSFPEAQEGVTGIGRRPFSQGLQQMMDHEARLLVATAYRHTEQVLRDNLDKLHALASALLEKEVINYEDIEALIGPPPHGPKKRIAPQKWSDAQREKQDSGEEEGA

Target ORF information:

RefSeq Version	XM_015100166.1
Organism	Ovis aries(sheep)
Definition	Ovis aries spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015100166.1

ORF Insert Sequence:

ATGACGATTG CCTTTCTAGT GCCGGAAAGC GGCGAAGGGA AACGGTCATC GAGAAGGGTC 
CGTGGGGGAA GGCGCATTAG AAGGCGCGTG TGGCGGGCCG CCGGCTGCGT CGGAGGCCCC 
GACCCGGGGC CCACCAGGCT GCAGTTCTAC TCGCAGCGCT TGTACTCGCA GTACAAACAA 
GGCTTGACAG GCCTCACTGG AAGAGGCCCG ACCGCGCAGG CGCCCCCGGG ACCGGGGCCG 
GGACCGGGGC CGGGGAGGCT GCGGTGCCCC TACTCGGGCT GGAACCCAGG CTGCTCCTCC 
GGGGCCAAGT GGAGGTGGCC GTATGTCGTC CACAGGCCTC CGATGGGCCT CGTCGGGCCT 
GGTGGCCGAG CCCTGCAGAG TTTCCAGTTG CGACTGCTAA CCCCTACCTT TGAAGGAATC 
AGCAGATTGT TGGTGAAACA GCACATTGTA CAGAATCCAG TCGGACTCTG GAAACTTCTA 
GGAGGCACTT ACTATTTTAA CACTTCGAGG AGGAAGCAGA AGACCAAGGA CAATGATAAA 
GCCAAGGGGA GGAGCCCTGA GGATGACGAA GAGGAGCGCA GGCGCCGGGA GCGCGAGGAC 
CAGATGTACC GAGAGCGGCT GCGCACCCTG TTCGTCATCG CAGTGATCAT GAGTCTGCTC 
AATGCGCTGG GCTCCAGCGG GGGAAACATC TCCTGGAATG ACTTCGTGCA CGAGATGCTG 
GCCAAGGGCG AGGTGCAGCG GGTGCAAGTG GTGCCTGAGA GCGACGTGGT GGAGGTGTAC 
CTGCACCCCG GTGCCGTGGT GTTCGGGCGG CCTCGGCTGG CATTGATGTA CCGAATGCAG 
GTGGCAAACA TCGACAAATT TGAAGAGAAG CTTCGAGCAG CTGAGGATGA GCTGAATATT 
GAAGGCAAGG ACAGGATTCC AGTTTCCTAC AAGCGGACGG GATTCTTTGG AAATGCTCTC 
TACGCCCTGG GGATGACAGC CGTGGGCTTG GCCATCCTGT GGTACGTCTT CCGTCTGGCT 
GGGATGACAG GACGGGAAGG CGGATTCAGT GCCTTTAATC AGCTGAAAAT GGCTCGTTTC 
ACGATTGTGG ATGGCAAGAT GGGGAAAGGA GTCAGCTTCA AAGATGTGGC AGGAATGCAT 
GAGGCCAAGC TGGAGGTCAA GGAGTTCGTG GATTATCTGA AGAGCCCGGA GCGCTTCCTT 
CAGCTTGGCG CCAAGGTCCC GAAGGGCGCA CTGCTGCTCG GCCCCCCCGG CTGCGGGAAG 
ACGCTACTGG CCAAAGCAGT GGCCACGGAG GCCCAGGTGC CCTTCCTGGC GATGGCTGGC 
CCAGAGTTCG TGGAGGTCAT TGGAGGCCTC GGTGCCGCCC GTGTGCGGAG CCTCTTCAAG 
GAAGCCCGGG CCCGGGCCCC CTGCATTGTG TACATTGACG AGATCGACGC CGTGGGCAAG 
AAGCGTTCCA CCGCCGTGTC CGGTTTCTCC AACACAGAGG AGGAGCAGAC GCTCAACCAG 
CTGCTGGTGG AGATGGACGG AATGGGTACC ACCGACCACG TCATCGTCCT GGCGGCCACC 
AACCGAGCTG ACATTTTGGA CAACGCTCTG CTGAGGCCAG GCCGACTGGA CAGGCATGTT 
TTCATCGACC TTCCCACTCT GCAGGAAAGG AAGGAGATCT TTGAGCAGCA CCTGAAGAGC 
CTGAAGCTGA CTCGGGCCAG CAGCTTTTAC TCCCAGCCCC TGGCAACCCC CACCCCTGTG 
CTGACAGGCG CTGACATTGC CAACATCTGT AATGAGGCTG CGTTGCACGC GGCGCGGGAG 
GGGCACACAG CTGTACACAC GTCCAACCTG GACTATGCCG TGGAGCGCGT CGTGGCCGGG 
ACTGCCAAGA AGAGCAAAGT CCTTTCCAAG GAAGAGCAGA AGGTGGTTGC TTTCCATGAG 
TCGGGCCACG CCCTGGTGGG CTGGCTGCTG GAGCACACGG AGGCTGTGAT GAAGGTGTCC 
ATCACTCCTA GGACCAACGC AGCCCTGGGC TTTGCTCAGA TGCTCCCGAG AGACCAGCAC 
CTCTTCACCA GGGAGCAGCT ATTTGAGCGC ATGTGCATGG CCCTGGGCGG CCGCGCATCC 
GAGTCCATCT CTTTCAACAG GGTCACTTCC GGGGCACAGG ACGACCTGCG GAAGGTCACC 
CGCATCGCCT ACTCCATGGT GAAGCAGTTT GGGATGGCGC CCAGCATTGG GCCCGTCTCC 
TTCCCCGAGG CCCAGGAGGG CGTCACGGGC ATCGGACGGC GTCCATTCAG CCAGGGCCTC 
CAGCAGATGA TGGACCACGA AGCGAGGCTG CTGGTGGCCA CTGCCTATAG GCATACGGAA 
CAGGTGCTTC GGGACAACCT GGACAAGCTC CACGCGCTGG CGAGTGCCCT GCTGGAAAAG 
GAAGTGATCA ACTACGAGGA CATCGAGGCC CTCATCGGCC CACCGCCCCA CGGGCCCAAG 
AAAAGGATCG CGCCGCAGAA GTGGAGTGAT GCCCAGAGAG AGAAGCAGGA CTCGGGGGAG 
GAGGAGGGGG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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SPG7 cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム