EPRS cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

Gene
Clones
gRNAs

Summary

Gene Symbol	EPRS
Entrez Gene ID	101104590
Full Name	glutamyl-prolyl-tRNA synthetase
Gene Type	protein-coding
Organism	Ovis aries musimon(Ovis orientalis musimon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004013603.4	XP_004013652.2	bifunctional glutamate/proline--tRNA ligase
XM_004013603.3	XP_004013652.1	bifunctional glutamate/proline--tRNA ligase isoform X2
XM_012147086.2	XP_012002476.1	bifunctional glutamate/proline--tRNA ligase isoform X1
XM_012187055.2	XP_012042445.1	bifunctional glutamate/proline--tRNA ligase isoform X1
XM_012147087.2	XP_012002477.1	bifunctional glutamate/proline--tRNA ligase isoform X2

Pathways from BioSystems
Gene Ontology

The following EPRS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPRS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv24208	XM_012147086.2 Latest version!	Ovis aries musimon glutamyl-prolyl-tRNA synthetase (EPRS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv24209	XM_012147087.2 Latest version!	Ovis aries musimon glutamyl-prolyl-tRNA synthetase (EPRS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OSm62291	XM_004013603.4 Latest version!	Ovis aries glutamyl-prolyl-tRNA synthetase (EPRS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm10564	XM_004013603.3 Latest version!	Ovis aries glutamyl-prolyl-tRNA synthetase (EPRS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OSm25588	XM_012187055.2 Latest version!	Ovis aries glutamyl-prolyl-tRNA synthetase (EPRS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EPRS ( XM_012147086.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012002476.1 Ovis aries musimon glutamyl-prolyl-tRNA synthetase (EPRS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOv24208
Clone ID Related Accession (Same CDS sequence)	XM_012147086.2
Accession Version	XM_012147086.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	bifunctional glutamate/proline--tRNA ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012147086.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGC TCTGTCTGAC TGTGAATTCG GGAGACCCTC CGCTAGGAGC TTTGTTGGCA 
GTGGAACATG TGAAAGACAA CGTCAGCATT TCTGTTGAAG AAGGGAAAGA GAATATTCTT 
CATGTATCTG AAAATGTGGT GTTCACGGAC ATAAACTCCA TACTTCGCTA CTTGGCTAGA 
ATTGCAACTA CAGCAGGGCT CTATGGCTCT AACTTGTTGG AACATACTGA GATTGATCAC 
TGGTTGGAGT TCAGTGCCAC GAAATTGTCT TCTTGTGATT TGTTTACTTC TGCAGTCAAC 
GAGCTCAATC ATTGTCTGTC TCTGAGAACA TACTTAGTTG GAAACTCCTT GAGCTTAGCA 
GATTTATGTG TTTGGGCCAC CCTAAAAGGA AATGCTGCGT GGCAGGAACA GTTGGGACAG 
AACAAAGCTC CAGTTCATGT AAAACGGTGG TTTGGCTTTC TTGAAGCCCA GCAGGCCTTC 
CAGTCAGTAG GTACCAAGTG GAATGTTTCA GCAACCAAAG CAAAAGTGGT TCCTGAGAAA 
AAGCAAGATG TTGGGAAATT TGTTGAGCTT CCAGGTGCTG AGATGGGGAA GGTTACAGTC 
AGATTTCCTC CAGAGGCTAG TGGTTACTTG CACATCGGGC ACGCAAAAGC TGCTCTCCTG 
AACCAGCACT ACCAGGTCAA CTTTAAAGGG AAACTGATCA TGAGATTCGA TGACACAAAT 
CCCGAAAAAG AAAAGGAAGA TTTTGAGAAG GTTATTTTGG AGGATGTTGC AATGTTGCAT 
ATCAAACCAG ATCAATTTAC TTACACCTCG GATCATTTTG AAACTATAAT GAAATATGCA 
GAAAAGCTAA TTCAGGAGGG GAAGGCTTAT GTCGACGATA CTCCTGCCGA GCAGATGAAA 
GCGGAGCGTG AGCAGAGGAT AGAATCCAAA CATAGAAAAA ACTCTGTGGA GAAGAATCTG 
CAAATGTGGG AAGAAATGAA AAAAGGAAGC CAGTTTGGTC AGTCCTGTTG TTTGCGAGCA 
AAAATTGACA TGAGTAGTAA CAATGGCTGC ATGAGGGACC CGACACTTTA TCGCTGCAAG 
ATTCAACCCC ACCCAAGAAC TGGGAATAAA TACAATGTTT ATCCGACATA TGATTTTGCC 
TGCCCCATAG TTGACAGCAT TGAAGGTGTT ACACACGCCC TGAGAACAAC AGAATACCAT 
GACAGAGATG AACAGTTCTA CTGGATTATT GAGGCGTTGG GCATAAGAAA ACCATTTATT 
TGGGAATACA GTCGGCTGAA TCTCAACAAC ACAGTGCTGT CCAAAAGAAA ACTCACGTGG 
TTTGTCAATG AAGGACTAGT AGATGGATGG GATGACCCAC GGTTTCCTAC AGTTCGTGGT 
GTGCTGAGAA GAGGGATGAC GGTCGAAGGA CTAAAACAGT TTATTGCTGC TCAGGGTTCT 
TCACGTTCAG TTGTGAACAT GGAATGGGAC AAAATTTGGG CATTTAACAA AAAGCTGCGA 
GCTCTCTGTA AGAAGGTTAT TGACCCAGTG GCTCCACGAT ACGTTGCATT ACTGAAGAAA 
GAGGTGGTCC CAGTGAACGT CCCCGAGGCC CAGGAGGAGA TGAAGGAAGT CGCCAAACAC 
CCGAAGAATC CTGATGTTGG CTTGAAGCCT GTGTGGTATA GTTCTAAAGT TTTCATCGAA 
GGTGCTGATG CAGAGACATT GTCAGAGGGT GAGATGGTTA CATTTATAAA CTGGGGCAAC 
ATCAACATTA CTAAAATACA CAAAAATGCA GAAGGCAAAA TTGTATCTCT TGACGCAAAA 
TTGAATTTAG AGAACAAAGA CTACAAGAAA ACCACTAAGA TCACTTGGCT TGCAGAGACG 
GAACACGCGC TTCCTGTCCC AGCAATCTGT GTCACGTACG AGCACTTGAT CACGAAGCCC 
GTGCTGGGAA AAGATGAGGA CTTTAAGCAG TATGTCAACA AAAACAGTAA GCATGAAGAG 
CTAATGCTGG GGGATCCCTG CCTTAAGGAT TTGAAAAAAG GAGACATTAT ACAACTCCAG 
AGAAGAGGCT TCTTCATATG TGATCAACCT TATGAACCCG TTAGTCCATA TAGCTGCATA 
GAAGCCCCAT GCATTCTGAT ATACATTCCT GATGGACATA CGAAGGAAAT GCCAACATCT 
GGGTCAAAGG AGAAGACCAA AGTAGAAACA ACAAAGAATG AGGCATCTAC ACCTCTCAAG 
ACAAGATCGG CTCCTTCTTT GAGTACTTGT GCTACATCTG AGGATTCCTT GGTCCTTTAC 
AACAGAGTTG CTGCCCAAGG GGACGTGGTT CGTGAATTAA AAGCCAAGAA AGCCACAAAG 
GAAGATATAG ATGCAGCTGT AAAACAGCTT TTGGCTTTGA AGGCTGAATA TAAGGAGAAA 
ACTGGCCAGG AATATAAGCC TGGAAATCCT CCCACTGCAG CAGGACAGAA TGTTTCTTCT 
AAATCATCAT CAAATATTGC GGAAAGTAAA TCTCTGTATG ATGAAGTTGC CGCACAAGGA 
GAAGTGGTCC GTAAACTGAA AGCCGAGAAA GCCCCTAAGG CTAAAGTAAA TGAAGCTGTA 
GAATGCTTAC TGTCCCTAAA AGCTCAGTAT AAAGAAAAAA CTGGGAAGGA GTACGTTCCT 
GGTCAGCCCC CATCATCTCA AAGTTCAGAT TCAAGTCCAC CCAGAAGCTC TGAGCCTAGT 
GGTCCAGAAA CACCAGAAGC CAAACTACTT TTTGACAGAG TAGCTTCTCA AGGAGAAGTA 
GTTCGAAAAC TTAAAGCTGA AAAAGCTTCT AAGGATCAAG TAGATACAGC CGTACAAGAC 
CTCCTTCAGC TGAAAGCACA GTACAGGTCT CTGACAGGAG TAGACTATAA GCCTGTCTCT 
GCCACTGGGG CTGAGGAGAA AGATAAGAAA AAGAAAGAAA AGGAGAATAA ATCTGAAAAG 
CAGAATAAGC CTCAGAAGCA AAATGACACC CAAAAGAAAG ACTTCTCTAA AAACCAAGGA 
AGTGGGCTGT CACCAAATGG AGCAGGGGAA GGACAAGGGC CTAAGAAGCA GACCAGGTTG 
GGTCTTGAGG CCAGAAAAGA AGACAGTCTT GCAGATTGGT ATTCTCAAGT CATCACAAAG 
TCAGAAATGA TTGAATACCA TGATGTAAGT GGCTGCTATA TCCTTCGTCC CTGGGCATAT 
TCCATTTGGG AATCCATCAA GGACTTTTTT GATGCTGAGA TCAAGAAACT CGGTGTTGAA 
AACTGCTATT TCCCCATGTT TGTGTCTCAA GGTGCATTAG AGAAAGAGAA GACTCATATT 
GCTGACTTTG CTCCTGAGGT TGCTTGGGTG ACAAGATCTG GCAAAACAGA GTTGGCAGAA 
CCAATTGCCA TTCGTCCTAC CAGTGAAACA GTAATGTATC CTGCATATGC GAAGTGGGTG 
CAGTCCCACA GAGACCTGCC CATAAAGCTC AACCAGTGGT GCAACGTGGT GCGTTGGGAG 
TTCAAGCACC CTCAGCCTTT CTTACGTACT CGTGAATTCC TCTGGCAGGA AGGGCACAGT 
GCCTTTGCTA CATTTGAAGA GGCAGCAGAA GAGGTTTTGC AGATACTTGA CTTATACGCT 
CAGGTATATG AAGAACTCCT GGCAATTCCT GTTGTAAAGG GAAGAAAGAC TGAAAAGGAG 
AAATTTGCGG GAGGAGATTA CACCACTACA GTGGAAGCAT TTATATCTGC CAGTGGAAGA 
GCTATCCAGG GAGCAACATC ACATCATTTA GGACAGAATT TCTCGAAAAT GTTTGAAATC 
GTTTTTGAAG ATCCAAAGAC AGCAGGAGAG AAGCAGTTGG CCTATCAGAA CTCCTGGGGC 
CTGACGACTC GAACTATCGG TGTGATGACC ATGGTTCACG GGGACAACGT GGGCTTAGTG 
CTGCCGCCCC GTGTAGCCTG TGTTCAGGTG GTGGTTATTC CCTGTGGCAT CACAAATGCA 
CTTTCTGAAG AAGACAGGGA TGCTCTACTT GCAAAATGCA ATGATTATCG AAGGCGGTTA 
CTCAGTGTCA ATATTCGAGT TCGAGTTGAT TTGCGAGATA ATTACTCACC AGGTTGGAAA 
TTCAATCACT GGGAACTCAA GGGAGTCCCG ATTAGACTTG AAGTCGGGCC ACGTGATATG 
AAGAACTGCC AGTTTGTAGC TGTCAGACGA GACACTGGGG AAAAGCTGAC AATTGCTGAA 
AATGAGGCTG AGACGAAGCT TCGTGCTATT TTGGAAGACA TCCACATTAA CCTTTTTACC 
CGGGCTTCTG AAGACCTTAA GACTCATATG GTTGTGGCTA ATACAATGGA GGACTTCCAG 
AAGGAGCTCG ATTCTGGGAA GATTGCTCAG ATTCCATTCT GTGGGGAAAT TGACTGTGAA 
GATTGGATCA AAAAGACTAC TGCCAGGGAT CAAGATCTTG AGCCTGGTGC TCCATCGATG 
GGAGCTAAAA GCCTGTGCAT TCCCTTCAAG CCACTCTGCG AGCTGCCGCC AGGAGCCAAG 
TGTGTGTGTG GAAAGAACCC TGCCAAGTAC TACACCTTGT TCGGTCGTAG TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012002476.1
CDS	142..4698
Translation	MATLCLTVNSGDPPLGALLAVEHVKDNVSISVEEGKENILHVSENVVFTDINSILRYLARIATTAGLYGSNLLEHTEIDHWLEFSATKLSSCDLFTSAVNELNHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLGQNKAPVHVKRWFGFLEAQQAFQSVGTKWNVSATKAKVVPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRIESKHRKNSVEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPFIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKLRALCKKVIDPVAPRYVALLKKEVVPVNVPEAQEEMKEVAKHPKNPDVGLKPVWYSSKVFIEGADAETLSEGEMVTFINWGNINITKIHKNAEGKIVSLDAKLNLENKDYKKTTKITWLAETEHALPVPAICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCIEAPCILIYIPDGHTKEMPTSGSKEKTKVETTKNEASTPLKTRSAPSLSTCATSEDSLVLYNRVAAQGDVVRELKAKKATKEDIDAAVKQLLALKAEYKEKTGQEYKPGNPPTAAGQNVSSKSSSNIAESKSLYDEVAAQGEVVRKLKAEKAPKAKVNEAVECLLSLKAQYKEKTGKEYVPGQPPSSQSSDSSPPRSSEPSGPETPEAKLLFDRVASQGEVVRKLKAEKASKDQVDTAVQDLLQLKAQYRSLTGVDYKPVSATGAEEKDKKKKEKENKSEKQNKPQKQNDTQKKDFSKNQGSGLSPNGAGEGQGPKKQTRLGLEARKEDSLADWYSQVITKSEMIEYHDVSGCYILRPWAYSIWESIKDFFDAEIKKLGVENCYFPMFVSQGALEKEKTHIADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATFEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTVEAFISASGRAIQGATSHHLGQNFSKMFEIVFEDPKTAGEKQLAYQNSWGLTTRTIGVMTMVHGDNVGLVLPPRVACVQVVVIPCGITNALSEEDRDALLAKCNDYRRRLLSVNIRVRVDLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKNCQFVAVRRDTGEKLTIAENEAETKLRAILEDIHINLFTRASEDLKTHMVVANTMEDFQKELDSGKIAQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELPPGAKCVCGKNPAKYYTLFGRSY

Target ORF information:

RefSeq Version	XM_012147086.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon glutamyl-prolyl-tRNA synthetase (EPRS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012147086.2

ORF Insert Sequence:

ATGGCGACGC TCTGTCTGAC TGTGAATTCG GGAGACCCTC CGCTAGGAGC TTTGTTGGCA 
GTGGAACATG TGAAAGACAA CGTCAGCATT TCTGTTGAAG AAGGGAAAGA GAATATTCTT 
CATGTATCTG AAAATGTGGT GTTCACGGAC ATAAACTCCA TACTTCGCTA CTTGGCTAGA 
ATTGCAACTA CAGCAGGGCT CTATGGCTCT AACTTGTTGG AACATACTGA GATTGATCAC 
TGGTTGGAGT TCAGTGCCAC GAAATTGTCT TCTTGTGATT TGTTTACTTC TGCAGTCAAC 
GAGCTCAATC ATTGTCTGTC TCTGAGAACA TACTTAGTTG GAAACTCCTT GAGCTTAGCA 
GATTTATGTG TTTGGGCCAC CCTAAAAGGA AATGCTGCGT GGCAGGAACA GTTGGGACAG 
AACAAAGCTC CAGTTCATGT AAAACGGTGG TTTGGCTTTC TTGAAGCCCA GCAGGCCTTC 
CAGTCAGTAG GTACCAAGTG GAATGTTTCA GCAACCAAAG CAAAAGTGGT TCCTGAGAAA 
AAGCAAGATG TTGGGAAATT TGTTGAGCTT CCAGGTGCTG AGATGGGGAA GGTTACAGTC 
AGATTTCCTC CAGAGGCTAG TGGTTACTTG CACATCGGGC ACGCAAAAGC TGCTCTCCTG 
AACCAGCACT ACCAGGTCAA CTTTAAAGGG AAACTGATCA TGAGATTCGA TGACACAAAT 
CCCGAAAAAG AAAAGGAAGA TTTTGAGAAG GTTATTTTGG AGGATGTTGC AATGTTGCAT 
ATCAAACCAG ATCAATTTAC TTACACCTCG GATCATTTTG AAACTATAAT GAAATATGCA 
GAAAAGCTAA TTCAGGAGGG GAAGGCTTAT GTCGACGATA CTCCTGCCGA GCAGATGAAA 
GCGGAGCGTG AGCAGAGGAT AGAATCCAAA CATAGAAAAA ACTCTGTGGA GAAGAATCTG 
CAAATGTGGG AAGAAATGAA AAAAGGAAGC CAGTTTGGTC AGTCCTGTTG TTTGCGAGCA 
AAAATTGACA TGAGTAGTAA CAATGGCTGC ATGAGGGACC CGACACTTTA TCGCTGCAAG 
ATTCAACCCC ACCCAAGAAC TGGGAATAAA TACAATGTTT ATCCGACATA TGATTTTGCC 
TGCCCCATAG TTGACAGCAT TGAAGGTGTT ACACACGCCC TGAGAACAAC AGAATACCAT 
GACAGAGATG AACAGTTCTA CTGGATTATT GAGGCGTTGG GCATAAGAAA ACCATTTATT 
TGGGAATACA GTCGGCTGAA TCTCAACAAC ACAGTGCTGT CCAAAAGAAA ACTCACGTGG 
TTTGTCAATG AAGGACTAGT AGATGGATGG GATGACCCAC GGTTTCCTAC AGTTCGTGGT 
GTGCTGAGAA GAGGGATGAC GGTCGAAGGA CTAAAACAGT TTATTGCTGC TCAGGGTTCT 
TCACGTTCAG TTGTGAACAT GGAATGGGAC AAAATTTGGG CATTTAACAA AAAGCTGCGA 
GCTCTCTGTA AGAAGGTTAT TGACCCAGTG GCTCCACGAT ACGTTGCATT ACTGAAGAAA 
GAGGTGGTCC CAGTGAACGT CCCCGAGGCC CAGGAGGAGA TGAAGGAAGT CGCCAAACAC 
CCGAAGAATC CTGATGTTGG CTTGAAGCCT GTGTGGTATA GTTCTAAAGT TTTCATCGAA 
GGTGCTGATG CAGAGACATT GTCAGAGGGT GAGATGGTTA CATTTATAAA CTGGGGCAAC 
ATCAACATTA CTAAAATACA CAAAAATGCA GAAGGCAAAA TTGTATCTCT TGACGCAAAA 
TTGAATTTAG AGAACAAAGA CTACAAGAAA ACCACTAAGA TCACTTGGCT TGCAGAGACG 
GAACACGCGC TTCCTGTCCC AGCAATCTGT GTCACGTACG AGCACTTGAT CACGAAGCCC 
GTGCTGGGAA AAGATGAGGA CTTTAAGCAG TATGTCAACA AAAACAGTAA GCATGAAGAG 
CTAATGCTGG GGGATCCCTG CCTTAAGGAT TTGAAAAAAG GAGACATTAT ACAACTCCAG 
AGAAGAGGCT TCTTCATATG TGATCAACCT TATGAACCCG TTAGTCCATA TAGCTGCATA 
GAAGCCCCAT GCATTCTGAT ATACATTCCT GATGGACATA CGAAGGAAAT GCCAACATCT 
GGGTCAAAGG AGAAGACCAA AGTAGAAACA ACAAAGAATG AGGCATCTAC ACCTCTCAAG 
ACAAGATCGG CTCCTTCTTT GAGTACTTGT GCTACATCTG AGGATTCCTT GGTCCTTTAC 
AACAGAGTTG CTGCCCAAGG GGACGTGGTT CGTGAATTAA AAGCCAAGAA AGCCACAAAG 
GAAGATATAG ATGCAGCTGT AAAACAGCTT TTGGCTTTGA AGGCTGAATA TAAGGAGAAA 
ACTGGCCAGG AATATAAGCC TGGAAATCCT CCCACTGCAG CAGGACAGAA TGTTTCTTCT 
AAATCATCAT CAAATATTGC GGAAAGTAAA TCTCTGTATG ATGAAGTTGC CGCACAAGGA 
GAAGTGGTCC GTAAACTGAA AGCCGAGAAA GCCCCTAAGG CTAAAGTAAA TGAAGCTGTA 
GAATGCTTAC TGTCCCTAAA AGCTCAGTAT AAAGAAAAAA CTGGGAAGGA GTACGTTCCT 
GGTCAGCCCC CATCATCTCA AAGTTCAGAT TCAAGTCCAC CCAGAAGCTC TGAGCCTAGT 
GGTCCAGAAA CACCAGAAGC CAAACTACTT TTTGACAGAG TAGCTTCTCA AGGAGAAGTA 
GTTCGAAAAC TTAAAGCTGA AAAAGCTTCT AAGGATCAAG TAGATACAGC CGTACAAGAC 
CTCCTTCAGC TGAAAGCACA GTACAGGTCT CTGACAGGAG TAGACTATAA GCCTGTCTCT 
GCCACTGGGG CTGAGGAGAA AGATAAGAAA AAGAAAGAAA AGGAGAATAA ATCTGAAAAG 
CAGAATAAGC CTCAGAAGCA AAATGACACC CAAAAGAAAG ACTTCTCTAA AAACCAAGGA 
AGTGGGCTGT CACCAAATGG AGCAGGGGAA GGACAAGGGC CTAAGAAGCA GACCAGGTTG 
GGTCTTGAGG CCAGAAAAGA AGACAGTCTT GCAGATTGGT ATTCTCAAGT CATCACAAAG 
TCAGAAATGA TTGAATACCA TGATGTAAGT GGCTGCTATA TCCTTCGTCC CTGGGCATAT 
TCCATTTGGG AATCCATCAA GGACTTTTTT GATGCTGAGA TCAAGAAACT CGGTGTTGAA 
AACTGCTATT TCCCCATGTT TGTGTCTCAA GGTGCATTAG AGAAAGAGAA GACTCATATT 
GCTGACTTTG CTCCTGAGGT TGCTTGGGTG ACAAGATCTG GCAAAACAGA GTTGGCAGAA 
CCAATTGCCA TTCGTCCTAC CAGTGAAACA GTAATGTATC CTGCATATGC GAAGTGGGTG 
CAGTCCCACA GAGACCTGCC CATAAAGCTC AACCAGTGGT GCAACGTGGT GCGTTGGGAG 
TTCAAGCACC CTCAGCCTTT CTTACGTACT CGTGAATTCC TCTGGCAGGA AGGGCACAGT 
GCCTTTGCTA CATTTGAAGA GGCAGCAGAA GAGGTTTTGC AGATACTTGA CTTATACGCT 
CAGGTATATG AAGAACTCCT GGCAATTCCT GTTGTAAAGG GAAGAAAGAC TGAAAAGGAG 
AAATTTGCGG GAGGAGATTA CACCACTACA GTGGAAGCAT TTATATCTGC CAGTGGAAGA 
GCTATCCAGG GAGCAACATC ACATCATTTA GGACAGAATT TCTCGAAAAT GTTTGAAATC 
GTTTTTGAAG ATCCAAAGAC AGCAGGAGAG AAGCAGTTGG CCTATCAGAA CTCCTGGGGC 
CTGACGACTC GAACTATCGG TGTGATGACC ATGGTTCACG GGGACAACGT GGGCTTAGTG 
CTGCCGCCCC GTGTAGCCTG TGTTCAGGTG GTGGTTATTC CCTGTGGCAT CACAAATGCA 
CTTTCTGAAG AAGACAGGGA TGCTCTACTT GCAAAATGCA ATGATTATCG AAGGCGGTTA 
CTCAGTGTCA ATATTCGAGT TCGAGTTGAT TTGCGAGATA ATTACTCACC AGGTTGGAAA 
TTCAATCACT GGGAACTCAA GGGAGTCCCG ATTAGACTTG AAGTCGGGCC ACGTGATATG 
AAGAACTGCC AGTTTGTAGC TGTCAGACGA GACACTGGGG AAAAGCTGAC AATTGCTGAA 
AATGAGGCTG AGACGAAGCT TCGTGCTATT TTGGAAGACA TCCACATTAA CCTTTTTACC 
CGGGCTTCTG AAGACCTTAA GACTCATATG GTTGTGGCTA ATACAATGGA GGACTTCCAG 
AAGGAGCTCG ATTCTGGGAA GATTGCTCAG ATTCCATTCT GTGGGGAAAT TGACTGTGAA 
GATTGGATCA AAAAGACTAC TGCCAGGGAT CAAGATCTTG AGCCTGGTGC TCCATCGATG 
GGAGCTAAAA GCCTGTGCAT TCCCTTCAAG CCACTCTGCG AGCTGCCGCC AGGAGCCAAG 
TGTGTGTGTG GAAAGAACCC TGCCAAGTAC TACACCTTGT TCGGTCGTAG TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPRS ( XM_012147087.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012002477.1 Ovis aries musimon glutamyl-prolyl-tRNA synthetase (EPRS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv24209
Clone ID Related Accession (Same CDS sequence)	XM_012147087.2
Accession Version	XM_012147087.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	bifunctional glutamate/proline--tRNA ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012147087.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGC TCTGTCTGAC TGTGAATTCG GGAGACCCTC CGCTAGGAGC TTTGTTGGCA 
GTGGAACATG TGAAAGACAA CGTCAGCATT TCTGTTGAAG AAGGGAAAGA GAATATTCTT 
CATGTATCTG AAAATGTGGT GTTCACGGAC ATAAACTCCA TACTTCGCTA CTTGGCTAGA 
ATTGCAACTA CAGCAGGGCT CTATGGCTCT AACTTGTTGG AACATACTGA GATTGATCAC 
TGGTTGGAGT TCAGTGCCAC GAAATTGTCT TCTTGTGATT TGTTTACTTC TGCAGTCAAC 
GAGCTCAATC ATTGTCTGTC TCTGAGAACA TACTTAGTTG GAAACTCCTT GAGCTTAGCA 
GATTTATGTG TTTGGGCCAC CCTAAAAGGA AATGCTGCGT GGCAGGAACA GTTGGGACAG 
AACAAAGCTC CAGTTCATGT AAAACGGTGG TTTGGCTTTC TTGAAGCCCA GCAGGCCTTC 
CAGTCAGTAG GTACCAAGTG GAATGTTTCA GCAACCAAAG CAAAAGTGGT TCCTGAGAAA 
AAGCAAGATG TTGGGAAATT TGTTGAGCTT CCAGGTGCTG AGATGGGGAA GGTTACAGTC 
AGATTTCCTC CAGAGGCTAG TGGTTACTTG CACATCGGGC ACGCAAAAGC TGCTCTCCTG 
AACCAGCACT ACCAGGTCAA CTTTAAAGGG AAACTGATCA TGAGATTCGA TGACACAAAT 
CCCGAAAAAG AAAAGGAAGA TTTTGAGAAG GTTATTTTGG AGGATGTTGC AATGTTGCAT 
ATCAAACCAG ATCAATTTAC TTACACCTCG GATCATTTTG AAACTATAAT GAAATATGCA 
GAAAAGCTAA TTCAGGAGGG GAAGGCTTAT GTCGACGATA CTCCTGCCGA GCAGATGAAA 
GCGGAGCGTG AGCAGAGGAT AGAATCCAAA CATAGAAAAA ACTCTGTGGA GAAGAATCTG 
CAAATGTGGG AAGAAATGAA AAAAGGAAGC CAGTTTGGTC AGTCCTGTTG TTTGCGAGCA 
AAAATTGACA TGAGTAGTAA CAATGGCTGC ATGAGGGACC CGACACTTTA TCGCTGCAAG 
ATTCAACCCC ACCCAAGAAC TGGGAATAAA TACAATGTTT ATCCGACATA TGATTTTGCC 
TGCCCCATAG TTGACAGCAT TGAAGGTGTT ACACACGCCC TGAGAACAAC AGAATACCAT 
GACAGAGATG AACAGTTCTA CTGGATTATT GAGGCGTTGG GCATAAGAAA ACCATTTATT 
TGGGAATACA GTCGGCTGAA TCTCAACAAC ACAGTGCTGT CCAAAAGAAA ACTCACGTGG 
TTTGTCAATG AAGGACTAGT AGATGGATGG GATGACCCAC GGTTTCCTAC AGTTCGTGGT 
GTGCTGAGAA GAGGGATGAC GGTCGAAGGA CTAAAACAGT TTATTGCTGC TCAGGGTTCT 
TCACGTTCAG TTGTGAACAT GGAATGGGAC AAAATTTGGG CATTTAACAA AAAGGTTATT 
GACCCAGTGG CTCCACGATA CGTTGCATTA CTGAAGAAAG AGGTGGTCCC AGTGAACGTC 
CCCGAGGCCC AGGAGGAGAT GAAGGAAGTC GCCAAACACC CGAAGAATCC TGATGTTGGC 
TTGAAGCCTG TGTGGTATAG TTCTAAAGTT TTCATCGAAG GTGCTGATGC AGAGACATTG 
TCAGAGGGTG AGATGGTTAC ATTTATAAAC TGGGGCAACA TCAACATTAC TAAAATACAC 
AAAAATGCAG AAGGCAAAAT TGTATCTCTT GACGCAAAAT TGAATTTAGA GAACAAAGAC 
TACAAGAAAA CCACTAAGAT CACTTGGCTT GCAGAGACGG AACACGCGCT TCCTGTCCCA 
GCAATCTGTG TCACGTACGA GCACTTGATC ACGAAGCCCG TGCTGGGAAA AGATGAGGAC 
TTTAAGCAGT ATGTCAACAA AAACAGTAAG CATGAAGAGC TAATGCTGGG GGATCCCTGC 
CTTAAGGATT TGAAAAAAGG AGACATTATA CAACTCCAGA GAAGAGGCTT CTTCATATGT 
GATCAACCTT ATGAACCCGT TAGTCCATAT AGCTGCATAG AAGCCCCATG CATTCTGATA 
TACATTCCTG ATGGACATAC GAAGGAAATG CCAACATCTG GGTCAAAGGA GAAGACCAAA 
GTAGAAACAA CAAAGAATGA GGCATCTACA CCTCTCAAGA CAAGATCGGC TCCTTCTTTG 
AGTACTTGTG CTACATCTGA GGATTCCTTG GTCCTTTACA ACAGAGTTGC TGCCCAAGGG 
GACGTGGTTC GTGAATTAAA AGCCAAGAAA GCCACAAAGG AAGATATAGA TGCAGCTGTA 
AAACAGCTTT TGGCTTTGAA GGCTGAATAT AAGGAGAAAA CTGGCCAGGA ATATAAGCCT 
GGAAATCCTC CCACTGCAGC AGGACAGAAT GTTTCTTCTA AATCATCATC AAATATTGCG 
GAAAGTAAAT CTCTGTATGA TGAAGTTGCC GCACAAGGAG AAGTGGTCCG TAAACTGAAA 
GCCGAGAAAG CCCCTAAGGC TAAAGTAAAT GAAGCTGTAG AATGCTTACT GTCCCTAAAA 
GCTCAGTATA AAGAAAAAAC TGGGAAGGAG TACGTTCCTG GTCAGCCCCC ATCATCTCAA 
AGTTCAGATT CAAGTCCACC CAGAAGCTCT GAGCCTAGTG GTCCAGAAAC ACCAGAAGCC 
AAACTACTTT TTGACAGAGT AGCTTCTCAA GGAGAAGTAG TTCGAAAACT TAAAGCTGAA 
AAAGCTTCTA AGGATCAAGT AGATACAGCC GTACAAGACC TCCTTCAGCT GAAAGCACAG 
TACAGGTCTC TGACAGGAGT AGACTATAAG CCTGTCTCTG CCACTGGGGC TGAGGAGAAA 
GATAAGAAAA AGAAAGAAAA GGAGAATAAA TCTGAAAAGC AGAATAAGCC TCAGAAGCAA 
AATGACACCC AAAAGAAAGA CTTCTCTAAA AACCAAGGAA GTGGGCTGTC ACCAAATGGA 
GCAGGGGAAG GACAAGGGCC TAAGAAGCAG ACCAGGTTGG GTCTTGAGGC CAGAAAAGAA 
GACAGTCTTG CAGATTGGTA TTCTCAAGTC ATCACAAAGT CAGAAATGAT TGAATACCAT 
GATGTAAGTG GCTGCTATAT CCTTCGTCCC TGGGCATATT CCATTTGGGA ATCCATCAAG 
GACTTTTTTG ATGCTGAGAT CAAGAAACTC GGTGTTGAAA ACTGCTATTT CCCCATGTTT 
GTGTCTCAAG GTGCATTAGA GAAAGAGAAG ACTCATATTG CTGACTTTGC TCCTGAGGTT 
GCTTGGGTGA CAAGATCTGG CAAAACAGAG TTGGCAGAAC CAATTGCCAT TCGTCCTACC 
AGTGAAACAG TAATGTATCC TGCATATGCG AAGTGGGTGC AGTCCCACAG AGACCTGCCC 
ATAAAGCTCA ACCAGTGGTG CAACGTGGTG CGTTGGGAGT TCAAGCACCC TCAGCCTTTC 
TTACGTACTC GTGAATTCCT CTGGCAGGAA GGGCACAGTG CCTTTGCTAC ATTTGAAGAG 
GCAGCAGAAG AGGTTTTGCA GATACTTGAC TTATACGCTC AGGTATATGA AGAACTCCTG 
GCAATTCCTG TTGTAAAGGG AAGAAAGACT GAAAAGGAGA AATTTGCGGG AGGAGATTAC 
ACCACTACAG TGGAAGCATT TATATCTGCC AGTGGAAGAG CTATCCAGGG AGCAACATCA 
CATCATTTAG GACAGAATTT CTCGAAAATG TTTGAAATCG TTTTTGAAGA TCCAAAGACA 
GCAGGAGAGA AGCAGTTGGC CTATCAGAAC TCCTGGGGCC TGACGACTCG AACTATCGGT 
GTGATGACCA TGGTTCACGG GGACAACGTG GGCTTAGTGC TGCCGCCCCG TGTAGCCTGT 
GTTCAGGTGG TGGTTATTCC CTGTGGCATC ACAAATGCAC TTTCTGAAGA AGACAGGGAT 
GCTCTACTTG CAAAATGCAA TGATTATCGA AGGCGGTTAC TCAGTGTCAA TATTCGAGTT 
CGAGTTGATT TGCGAGATAA TTACTCACCA GGTTGGAAAT TCAATCACTG GGAACTCAAG 
GGAGTCCCGA TTAGACTTGA AGTCGGGCCA CGTGATATGA AGAACTGCCA GTTTGTAGCT 
GTCAGACGAG ACACTGGGGA AAAGCTGACA ATTGCTGAAA ATGAGGCTGA GACGAAGCTT 
CGTGCTATTT TGGAAGACAT CCACATTAAC CTTTTTACCC GGGCTTCTGA AGACCTTAAG 
ACTCATATGG TTGTGGCTAA TACAATGGAG GACTTCCAGA AGGAGCTCGA TTCTGGGAAG 
ATTGCTCAGA TTCCATTCTG TGGGGAAATT GACTGTGAAG ATTGGATCAA AAAGACTACT 
GCCAGGGATC AAGATCTTGA GCCTGGTGCT CCATCGATGG GAGCTAAAAG CCTGTGCATT 
CCCTTCAAGC CACTCTGCGA GCTGCCGCCA GGAGCCAAGT GTGTGTGTGG AAAGAACCCT 
GCCAAGTACT ACACCTTGTT CGGTCGTAGT TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012002477.1
CDS	141..4676
Translation	MATLCLTVNSGDPPLGALLAVEHVKDNVSISVEEGKENILHVSENVVFTDINSILRYLARIATTAGLYGSNLLEHTEIDHWLEFSATKLSSCDLFTSAVNELNHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLGQNKAPVHVKRWFGFLEAQQAFQSVGTKWNVSATKAKVVPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRIESKHRKNSVEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPFIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVVPVNVPEAQEEMKEVAKHPKNPDVGLKPVWYSSKVFIEGADAETLSEGEMVTFINWGNINITKIHKNAEGKIVSLDAKLNLENKDYKKTTKITWLAETEHALPVPAICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCIEAPCILIYIPDGHTKEMPTSGSKEKTKVETTKNEASTPLKTRSAPSLSTCATSEDSLVLYNRVAAQGDVVRELKAKKATKEDIDAAVKQLLALKAEYKEKTGQEYKPGNPPTAAGQNVSSKSSSNIAESKSLYDEVAAQGEVVRKLKAEKAPKAKVNEAVECLLSLKAQYKEKTGKEYVPGQPPSSQSSDSSPPRSSEPSGPETPEAKLLFDRVASQGEVVRKLKAEKASKDQVDTAVQDLLQLKAQYRSLTGVDYKPVSATGAEEKDKKKKEKENKSEKQNKPQKQNDTQKKDFSKNQGSGLSPNGAGEGQGPKKQTRLGLEARKEDSLADWYSQVITKSEMIEYHDVSGCYILRPWAYSIWESIKDFFDAEIKKLGVENCYFPMFVSQGALEKEKTHIADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATFEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTVEAFISASGRAIQGATSHHLGQNFSKMFEIVFEDPKTAGEKQLAYQNSWGLTTRTIGVMTMVHGDNVGLVLPPRVACVQVVVIPCGITNALSEEDRDALLAKCNDYRRRLLSVNIRVRVDLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKNCQFVAVRRDTGEKLTIAENEAETKLRAILEDIHINLFTRASEDLKTHMVVANTMEDFQKELDSGKIAQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELPPGAKCVCGKNPAKYYTLFGRSY

Target ORF information:

RefSeq Version	XM_012147087.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon glutamyl-prolyl-tRNA synthetase (EPRS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012147087.2

ORF Insert Sequence:

ATGGCGACGC TCTGTCTGAC TGTGAATTCG GGAGACCCTC CGCTAGGAGC TTTGTTGGCA 
GTGGAACATG TGAAAGACAA CGTCAGCATT TCTGTTGAAG AAGGGAAAGA GAATATTCTT 
CATGTATCTG AAAATGTGGT GTTCACGGAC ATAAACTCCA TACTTCGCTA CTTGGCTAGA 
ATTGCAACTA CAGCAGGGCT CTATGGCTCT AACTTGTTGG AACATACTGA GATTGATCAC 
TGGTTGGAGT TCAGTGCCAC GAAATTGTCT TCTTGTGATT TGTTTACTTC TGCAGTCAAC 
GAGCTCAATC ATTGTCTGTC TCTGAGAACA TACTTAGTTG GAAACTCCTT GAGCTTAGCA 
GATTTATGTG TTTGGGCCAC CCTAAAAGGA AATGCTGCGT GGCAGGAACA GTTGGGACAG 
AACAAAGCTC CAGTTCATGT AAAACGGTGG TTTGGCTTTC TTGAAGCCCA GCAGGCCTTC 
CAGTCAGTAG GTACCAAGTG GAATGTTTCA GCAACCAAAG CAAAAGTGGT TCCTGAGAAA 
AAGCAAGATG TTGGGAAATT TGTTGAGCTT CCAGGTGCTG AGATGGGGAA GGTTACAGTC 
AGATTTCCTC CAGAGGCTAG TGGTTACTTG CACATCGGGC ACGCAAAAGC TGCTCTCCTG 
AACCAGCACT ACCAGGTCAA CTTTAAAGGG AAACTGATCA TGAGATTCGA TGACACAAAT 
CCCGAAAAAG AAAAGGAAGA TTTTGAGAAG GTTATTTTGG AGGATGTTGC AATGTTGCAT 
ATCAAACCAG ATCAATTTAC TTACACCTCG GATCATTTTG AAACTATAAT GAAATATGCA 
GAAAAGCTAA TTCAGGAGGG GAAGGCTTAT GTCGACGATA CTCCTGCCGA GCAGATGAAA 
GCGGAGCGTG AGCAGAGGAT AGAATCCAAA CATAGAAAAA ACTCTGTGGA GAAGAATCTG 
CAAATGTGGG AAGAAATGAA AAAAGGAAGC CAGTTTGGTC AGTCCTGTTG TTTGCGAGCA 
AAAATTGACA TGAGTAGTAA CAATGGCTGC ATGAGGGACC CGACACTTTA TCGCTGCAAG 
ATTCAACCCC ACCCAAGAAC TGGGAATAAA TACAATGTTT ATCCGACATA TGATTTTGCC 
TGCCCCATAG TTGACAGCAT TGAAGGTGTT ACACACGCCC TGAGAACAAC AGAATACCAT 
GACAGAGATG AACAGTTCTA CTGGATTATT GAGGCGTTGG GCATAAGAAA ACCATTTATT 
TGGGAATACA GTCGGCTGAA TCTCAACAAC ACAGTGCTGT CCAAAAGAAA ACTCACGTGG 
TTTGTCAATG AAGGACTAGT AGATGGATGG GATGACCCAC GGTTTCCTAC AGTTCGTGGT 
GTGCTGAGAA GAGGGATGAC GGTCGAAGGA CTAAAACAGT TTATTGCTGC TCAGGGTTCT 
TCACGTTCAG TTGTGAACAT GGAATGGGAC AAAATTTGGG CATTTAACAA AAAGGTTATT 
GACCCAGTGG CTCCACGATA CGTTGCATTA CTGAAGAAAG AGGTGGTCCC AGTGAACGTC 
CCCGAGGCCC AGGAGGAGAT GAAGGAAGTC GCCAAACACC CGAAGAATCC TGATGTTGGC 
TTGAAGCCTG TGTGGTATAG TTCTAAAGTT TTCATCGAAG GTGCTGATGC AGAGACATTG 
TCAGAGGGTG AGATGGTTAC ATTTATAAAC TGGGGCAACA TCAACATTAC TAAAATACAC 
AAAAATGCAG AAGGCAAAAT TGTATCTCTT GACGCAAAAT TGAATTTAGA GAACAAAGAC 
TACAAGAAAA CCACTAAGAT CACTTGGCTT GCAGAGACGG AACACGCGCT TCCTGTCCCA 
GCAATCTGTG TCACGTACGA GCACTTGATC ACGAAGCCCG TGCTGGGAAA AGATGAGGAC 
TTTAAGCAGT ATGTCAACAA AAACAGTAAG CATGAAGAGC TAATGCTGGG GGATCCCTGC 
CTTAAGGATT TGAAAAAAGG AGACATTATA CAACTCCAGA GAAGAGGCTT CTTCATATGT 
GATCAACCTT ATGAACCCGT TAGTCCATAT AGCTGCATAG AAGCCCCATG CATTCTGATA 
TACATTCCTG ATGGACATAC GAAGGAAATG CCAACATCTG GGTCAAAGGA GAAGACCAAA 
GTAGAAACAA CAAAGAATGA GGCATCTACA CCTCTCAAGA CAAGATCGGC TCCTTCTTTG 
AGTACTTGTG CTACATCTGA GGATTCCTTG GTCCTTTACA ACAGAGTTGC TGCCCAAGGG 
GACGTGGTTC GTGAATTAAA AGCCAAGAAA GCCACAAAGG AAGATATAGA TGCAGCTGTA 
AAACAGCTTT TGGCTTTGAA GGCTGAATAT AAGGAGAAAA CTGGCCAGGA ATATAAGCCT 
GGAAATCCTC CCACTGCAGC AGGACAGAAT GTTTCTTCTA AATCATCATC AAATATTGCG 
GAAAGTAAAT CTCTGTATGA TGAAGTTGCC GCACAAGGAG AAGTGGTCCG TAAACTGAAA 
GCCGAGAAAG CCCCTAAGGC TAAAGTAAAT GAAGCTGTAG AATGCTTACT GTCCCTAAAA 
GCTCAGTATA AAGAAAAAAC TGGGAAGGAG TACGTTCCTG GTCAGCCCCC ATCATCTCAA 
AGTTCAGATT CAAGTCCACC CAGAAGCTCT GAGCCTAGTG GTCCAGAAAC ACCAGAAGCC 
AAACTACTTT TTGACAGAGT AGCTTCTCAA GGAGAAGTAG TTCGAAAACT TAAAGCTGAA 
AAAGCTTCTA AGGATCAAGT AGATACAGCC GTACAAGACC TCCTTCAGCT GAAAGCACAG 
TACAGGTCTC TGACAGGAGT AGACTATAAG CCTGTCTCTG CCACTGGGGC TGAGGAGAAA 
GATAAGAAAA AGAAAGAAAA GGAGAATAAA TCTGAAAAGC AGAATAAGCC TCAGAAGCAA 
AATGACACCC AAAAGAAAGA CTTCTCTAAA AACCAAGGAA GTGGGCTGTC ACCAAATGGA 
GCAGGGGAAG GACAAGGGCC TAAGAAGCAG ACCAGGTTGG GTCTTGAGGC CAGAAAAGAA 
GACAGTCTTG CAGATTGGTA TTCTCAAGTC ATCACAAAGT CAGAAATGAT TGAATACCAT 
GATGTAAGTG GCTGCTATAT CCTTCGTCCC TGGGCATATT CCATTTGGGA ATCCATCAAG 
GACTTTTTTG ATGCTGAGAT CAAGAAACTC GGTGTTGAAA ACTGCTATTT CCCCATGTTT 
GTGTCTCAAG GTGCATTAGA GAAAGAGAAG ACTCATATTG CTGACTTTGC TCCTGAGGTT 
GCTTGGGTGA CAAGATCTGG CAAAACAGAG TTGGCAGAAC CAATTGCCAT TCGTCCTACC 
AGTGAAACAG TAATGTATCC TGCATATGCG AAGTGGGTGC AGTCCCACAG AGACCTGCCC 
ATAAAGCTCA ACCAGTGGTG CAACGTGGTG CGTTGGGAGT TCAAGCACCC TCAGCCTTTC 
TTACGTACTC GTGAATTCCT CTGGCAGGAA GGGCACAGTG CCTTTGCTAC ATTTGAAGAG 
GCAGCAGAAG AGGTTTTGCA GATACTTGAC TTATACGCTC AGGTATATGA AGAACTCCTG 
GCAATTCCTG TTGTAAAGGG AAGAAAGACT GAAAAGGAGA AATTTGCGGG AGGAGATTAC 
ACCACTACAG TGGAAGCATT TATATCTGCC AGTGGAAGAG CTATCCAGGG AGCAACATCA 
CATCATTTAG GACAGAATTT CTCGAAAATG TTTGAAATCG TTTTTGAAGA TCCAAAGACA 
GCAGGAGAGA AGCAGTTGGC CTATCAGAAC TCCTGGGGCC TGACGACTCG AACTATCGGT 
GTGATGACCA TGGTTCACGG GGACAACGTG GGCTTAGTGC TGCCGCCCCG TGTAGCCTGT 
GTTCAGGTGG TGGTTATTCC CTGTGGCATC ACAAATGCAC TTTCTGAAGA AGACAGGGAT 
GCTCTACTTG CAAAATGCAA TGATTATCGA AGGCGGTTAC TCAGTGTCAA TATTCGAGTT 
CGAGTTGATT TGCGAGATAA TTACTCACCA GGTTGGAAAT TCAATCACTG GGAACTCAAG 
GGAGTCCCGA TTAGACTTGA AGTCGGGCCA CGTGATATGA AGAACTGCCA GTTTGTAGCT 
GTCAGACGAG ACACTGGGGA AAAGCTGACA ATTGCTGAAA ATGAGGCTGA GACGAAGCTT 
CGTGCTATTT TGGAAGACAT CCACATTAAC CTTTTTACCC GGGCTTCTGA AGACCTTAAG 
ACTCATATGG TTGTGGCTAA TACAATGGAG GACTTCCAGA AGGAGCTCGA TTCTGGGAAG 
ATTGCTCAGA TTCCATTCTG TGGGGAAATT GACTGTGAAG ATTGGATCAA AAAGACTACT 
GCCAGGGATC AAGATCTTGA GCCTGGTGCT CCATCGATGG GAGCTAAAAG CCTGTGCATT 
CCCTTCAAGC CACTCTGCGA GCTGCCGCCA GGAGCCAAGT GTGTGTGTGG AAAGAACCCT 
GCCAAGTACT ACACCTTGTT CGGTCGTAGT TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPRS ( XM_004013603.4 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_004013652.2 Ovis aries glutamyl-prolyl-tRNA synthetase (EPRS), mRNA.

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CloneID	OSm62291
Clone ID Related Accession (Same CDS sequence)	XM_004013603.4
Accession Version	XM_004013603.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	bifunctional glutamate/proline--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_004013603.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGC TCTGTCTGAC TGTGAATTCG GGAGACCCTC CGCTAGGAGC TTTGTTGGCA 
GTGGAACATG TGAAAGACAA CGTCAGCATT TCTGTTGAAG AAGGGAAAGA GAATATTCTT 
CATGTATCTG AAAATGTGGT GTTCACGGAC ATAAACTCCA TACTTCGCTA CTTGGCTAGA 
ATTGCAACTA CAGCAGGGCT CTATGGCTCT AACTTGTTGG AACATACTGA GATTGATCAC 
TGGTTGGAGT TCAGTGCCAC GAAATTGTCT TCTTGTGATT TGTTTACTTC TGCAGTCAAC 
GAGCTCAATC ATTGTCTGTC TCTGAGAACA TACTTAGTTG GAAACTCCTT GAGCTTAGCA 
GATTTATGTG TTTGGGCCAC CCTAAAAGGA AATGCTGCGT GGCAGGAACA GTTGGGACAG 
AACAAAGCTC CAGTTCATGT AAAACGGTGG TTTGGCTTTC TTGAAGCCCA GCAGGCCTTC 
CAGTCAGTAG GTACCAAGTG GAATGTTTCA GCAACCAAAG CAAAAGTGGT TCCTGAGAAA 
AAGCAAGATG TTGGGAAATT TGTTGAGCTT CCAGGTGCTG AGATGGGGAA GGTTACAGTC 
AGATTTCCTC CAGAGGCTAG TGGTTACTTG CACATCGGGC ACGCAAAAGC TGCTCTCCTG 
AACCAGCACT ACCAGGTCAA CTTTAAAGGG AAACTGATCA TGAGATTCGA TGACACAAAT 
CCCGAAAAAG AAAAGGAAGA TTTTGAGAAG GTTATTTTGG AGGATGTTGC AATGTTGCAT 
ATCAAACCAG ATCAATTTAC TTACACCTCG GATCATTTTG AAACTATAAT GAAATATGCA 
GAAAAGCTAA TTCAGGAGGG GAAGGCTTAT GTCGACGATA CTCCTGCCGA GCAGATGAAA 
GCGGAGCGTG AGCAGAGGAT AGAATCCAAA CATAGAAAAA ACTCTGTGGA GAAGAATCTG 
CAAATGTGGG AAGAAATGAA AAAAGGAAGC CAGTTTGGTC AGTCCTGTTG TTTGCGAGCA 
AAAATTGACA TGAGTAGTAA CAATGGCTGC ATGAGGGACC CGACACTTTA TCGCTGCAAG 
ATTCAACCCC ACCCAAGAAC TGGGAATAAA TACAATGTTT ATCCGACATA TGATTTTGCC 
TGCCCCATAG TTGACAGCAT TGAAGGTGTT ACACACGCCC TGAGAACAAC AGAATACCAT 
GACAGAGATG AACAGTTCTA CTGGATTATT GAGGCATTGG GCATAAGAAA ACCATTTATT 
TGGGAATACA GTCGGCTGAA TCTCAACAAC ACAGTGCTGT CCAAAAGAAA ACTCACGTGG 
TTTGTCAATG AAGGACTAGT AGATGGATGG GATGACCCAC GGTTTCCTAC AGTTCGTGGT 
GTGCTGAGAA GAGGGATGAC GGTCGAAGGA CTAAAACAGT TTATTGCTGC TCAGGGTTCT 
TCACGTTCAG TTGTGAACAT GGAATGGGAC AAAATTTGGG CATTTAACAA AAAGGTTATT 
GACCCAGTGG CTCCACGATA CGTTGCATTA CTGAAGAAAG AGGTGGTCCC GGTGAACGTC 
CCCGAGGCCC AGGAGGAGAT GAAGGAAGTC GCCAAACACC CGAAGAATCC TGATGTTGGC 
TTGAAGCCTG TGTGGTATAG TTCTAAAGTT TTCATCGAAG GTGCTGATGC AGAGACATTG 
TCAGAGGGTG AGATGGTTAC ATTTATAAAC TGGGGCAACA TCAACATTAC TAAAATACAC 
AAAAATCCAG AAGGCAAAAT TGTATCTCTT GACGCAAAAT TGAATTTAGA GAACAAAGAC 
TACAAGAAAA CCACTAAGAT CACTTGGCTT GCAGAGACGG AACACGCGCT TCCTGTCCCA 
GCAATCTGTG TCACGTACGA GCACTTGATC ACGAAGCCCG TGCTGGGAAA AGATGAGGAC 
TTTAAGCAGT ATGTCAACAA AAACAGTAAG CATGAAGAAC TAATGCTGGG GGATCCCTGC 
CTTAAGGATT TGAAAAAAGG AGACATTATA CAACTCCAGA GAAGAGGCTT CTTCATATGT 
GATCAACCTT ATGAACCCGT TAGTCCATAT AGCTGCATAG AAGCCCCATG CATTCTGATA 
TACATTCCTG ATGGACATAC GAAGGAAATG CCAACATCTG GGTCAAAGGA GAAGACCAAA 
GTAGAAACAA CAAAGAATGA GGCATCTACA CCTCTCAAGA CAAGATCGGC TCCTTCTTTG 
AGTACTTGTG CTACATCTGA GGATTCCTTG GTCCTTTACA ACAGAGTTGC TGCCCAAGGG 
GACGTGGTTC GTGAATTAAA AGCCAAGAAA GCCACAAAGG AAGATATAGA TGCAGCTGTA 
AAACAGCTTT TGGCTTTGAA GGCTGAATAT AAGGAGAAAA CTGGCCAGGA ATATAAGCCT 
GGAAATCCTC CCGCTGCAGC AGGACAGAAT GTTTCTTCTA AATCATCATC AAATATTGTG 
GAAAGTAAAT CTCTGTATGA TGAAGTTGCC GCACAAGGAG AAGTGGTCCG TAAACTGAAA 
GCCGAGAAAG CCCCTAAGGC TAAAGTAAAT GAAGCTGTAG AATGCTTACT GTCCCTAAAA 
GCTCAGTATA AAGAAAAAAC TGGGAAGGAG TACGTTCCTG GTCAGCCCCC ATCATCTCAA 
AGTTCAGATT CAAGTCCACC CAGAAGCTCT GAGCCTAGTG GTCCAGAAAC ACCAGAAGCC 
AAACTACTTT TTGACAGAGT AGCTTCTCAA GGAGAAGTAG TTCGAAAACT TAAAGCTGAA 
AAAGCTTCTA AGGATCAAGT AGATACAGCC GTACAAGACC TCCTTCAGCT GAAAGCACAG 
TACAGGTCTC TGACAGGAGT AGACTATAAG CCTGTCTCTG CCACTGGGGC TGAGGAGAAA 
GATAAGAAAA AGAAAGAAAA GGAGAATAAA TCTGAAAAGC AGAATAAGCC TCAGAAGCAA 
AATGACACCC AAAAGAAAGA CTTCTCTAAA AACCAAGGAA GTGGGCTGTC ACCAAATGGA 
GCAGGGGAAG GACAAGGGCC TAAGAAGCAG ACCAGGTTGG GTCTTGAGGC CAGAAAAGAA 
GACAGTCTTG CAGATTGGTA TTCTCAAGTC ATCACAAAGT CAGAAATGAT TGAATACCAT 
GATGTAAGTG GCTGCTATAT CCTTCGTCCC TGGGCATATT CCATTTGGGA ATCCATCAAG 
GACTTTTTTG ATGCTGAGAT CAAGAAACTC GGTGTTGAAA ACTGCTATTT CCCCATGTTT 
GTGTCTCAAG GTGCATTAGA GAAAGAGAAG ACTCATATTG CTGACTTTGC TCCTGAGGTT 
GCTTGGGTGA CAAGATCTGG CAAAACAGAG TTGGCAGAAC CAATTGCCAT TCGTCCTACC 
AGTGAAACAG TAATGTATCC TGCATATGCG AAGTGGGTGC AGTCCCACAG AGACCTGCCC 
ATAAAGCTCA ACCAGTGGTG CAACGTGGTG CGTTGGGAGT TCAAGCACCC TCAGCCTTTC 
TTACGTACTC GTGAATTCCT CTGGCAGGAA GGGCACAGTG CCTTTGCTAC ATTTGAAGAG 
GCAGCAGAAG AGGTTTTGCA GATACTTGAC TTATACGCTC AGGTATATGA AGAACTCCTG 
GCAATTCCTG TTGTAAAGGG AAGAAAGACT GAAAAGGAGA AATTTGCGGG AGGAGATTAC 
ACCACTACAG TGGAAGCATT TATATCTGCC AGTGGAAGAG CTATCCAGGG AGCAACATCA 
CATCATTTAG GACAGAATTT CTCGAAAATG TTTGAAATCG TTTTTGAAGA TCCAAAGACA 
GCAGGAGAGA AGCAGTTGGC CTATCAGAAC TCCTGGGGCC TGACGACTCG AACTATCGGT 
GTGATGACCA TGGTTCACGG GGACAACGTG GGCTTAGTGC TGCCGCCCCG TGTAGCCTGT 
GTTCAGGTGG TGGTTATTCC CTGTGGCATC ACAAATGCAC TTTCTGAAGA AGACAGGGAT 
GCTCTACTTG CAAAATGCAA TGATTATAGA AGGCGGTTAC TCAGTGTCAA TATTCGAGTT 
CGAGTTGATT TGCGAGATAA TTACTCACCA GGTTGGAAAT TCAATCACTG GGAACTCAAG 
GGAGTCCCGA TTAGACTTGA AGTCGGGCCA CGTGATATGA AGAACTGCCA GTTTGTAGCT 
GTCAGACGAG ACACTGGGGA AAAGCTGACA ATTGCTGAAA ATGAGGCTGA GACGAAGCTT 
CGTGCTATTT TGGAAGACAT CCATGTTAAC CTCTTTACCC GGGCTTCTGA AGACCTTAAG 
ACTCATATGG TTGTGGCTAA TACAATGGAG GACTTCCAGA AGGAGCTCGA TTCTGGGAAG 
ATTGCTCAGA TTCCATTCTG TGGGGAAATT GACTGTGAAG ATTGGATCAA AAAGACTACT 
GCCAGGGATC AAGATCTTGA GCCCGGTGCT CCGTCGATGG GAGCTAAAAG CCTGTGCATT 
CCCTTCAAGC CACTCTGCGA GCTGCCGCCA GGAGCCAAGT GTGTGTGTGG AAAGAACCCT 
GCCAAGTACT ACACCTTGTT CGGTCGTAGT TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004013652.2
CDS	123..4658
Translation	MATLCLTVNSGDPPLGALLAVEHVKDNVSISVEEGKENILHVSENVVFTDINSILRYLARIATTAGLYGSNLLEHTEIDHWLEFSATKLSSCDLFTSAVNELNHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLGQNKAPVHVKRWFGFLEAQQAFQSVGTKWNVSATKAKVVPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRIESKHRKNSVEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPFIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVVPVNVPEAQEEMKEVAKHPKNPDVGLKPVWYSSKVFIEGADAETLSEGEMVTFINWGNINITKIHKNPEGKIVSLDAKLNLENKDYKKTTKITWLAETEHALPVPAICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCIEAPCILIYIPDGHTKEMPTSGSKEKTKVETTKNEASTPLKTRSAPSLSTCATSEDSLVLYNRVAAQGDVVRELKAKKATKEDIDAAVKQLLALKAEYKEKTGQEYKPGNPPAAAGQNVSSKSSSNIVESKSLYDEVAAQGEVVRKLKAEKAPKAKVNEAVECLLSLKAQYKEKTGKEYVPGQPPSSQSSDSSPPRSSEPSGPETPEAKLLFDRVASQGEVVRKLKAEKASKDQVDTAVQDLLQLKAQYRSLTGVDYKPVSATGAEEKDKKKKEKENKSEKQNKPQKQNDTQKKDFSKNQGSGLSPNGAGEGQGPKKQTRLGLEARKEDSLADWYSQVITKSEMIEYHDVSGCYILRPWAYSIWESIKDFFDAEIKKLGVENCYFPMFVSQGALEKEKTHIADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATFEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTVEAFISASGRAIQGATSHHLGQNFSKMFEIVFEDPKTAGEKQLAYQNSWGLTTRTIGVMTMVHGDNVGLVLPPRVACVQVVVIPCGITNALSEEDRDALLAKCNDYRRRLLSVNIRVRVDLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKNCQFVAVRRDTGEKLTIAENEAETKLRAILEDIHVNLFTRASEDLKTHMVVANTMEDFQKELDSGKIAQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELPPGAKCVCGKNPAKYYTLFGRSY

Target ORF information:

RefSeq Version	XM_004013603.4
Organism	Ovis aries(sheep)
Definition	Ovis aries glutamyl-prolyl-tRNA synthetase (EPRS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004013603.4

ORF Insert Sequence:

ATGGCGACGC TCTGTCTGAC TGTGAATTCG GGAGACCCTC CGCTAGGAGC TTTGTTGGCA 
GTGGAACATG TGAAAGACAA CGTCAGCATT TCTGTTGAAG AAGGGAAAGA GAATATTCTT 
CATGTATCTG AAAATGTGGT GTTCACGGAC ATAAACTCCA TACTTCGCTA CTTGGCTAGA 
ATTGCAACTA CAGCAGGGCT CTATGGCTCT AACTTGTTGG AACATACTGA GATTGATCAC 
TGGTTGGAGT TCAGTGCCAC GAAATTGTCT TCTTGTGATT TGTTTACTTC TGCAGTCAAC 
GAGCTCAATC ATTGTCTGTC TCTGAGAACA TACTTAGTTG GAAACTCCTT GAGCTTAGCA 
GATTTATGTG TTTGGGCCAC CCTAAAAGGA AATGCTGCGT GGCAGGAACA GTTGGGACAG 
AACAAAGCTC CAGTTCATGT AAAACGGTGG TTTGGCTTTC TTGAAGCCCA GCAGGCCTTC 
CAGTCAGTAG GTACCAAGTG GAATGTTTCA GCAACCAAAG CAAAAGTGGT TCCTGAGAAA 
AAGCAAGATG TTGGGAAATT TGTTGAGCTT CCAGGTGCTG AGATGGGGAA GGTTACAGTC 
AGATTTCCTC CAGAGGCTAG TGGTTACTTG CACATCGGGC ACGCAAAAGC TGCTCTCCTG 
AACCAGCACT ACCAGGTCAA CTTTAAAGGG AAACTGATCA TGAGATTCGA TGACACAAAT 
CCCGAAAAAG AAAAGGAAGA TTTTGAGAAG GTTATTTTGG AGGATGTTGC AATGTTGCAT 
ATCAAACCAG ATCAATTTAC TTACACCTCG GATCATTTTG AAACTATAAT GAAATATGCA 
GAAAAGCTAA TTCAGGAGGG GAAGGCTTAT GTCGACGATA CTCCTGCCGA GCAGATGAAA 
GCGGAGCGTG AGCAGAGGAT AGAATCCAAA CATAGAAAAA ACTCTGTGGA GAAGAATCTG 
CAAATGTGGG AAGAAATGAA AAAAGGAAGC CAGTTTGGTC AGTCCTGTTG TTTGCGAGCA 
AAAATTGACA TGAGTAGTAA CAATGGCTGC ATGAGGGACC CGACACTTTA TCGCTGCAAG 
ATTCAACCCC ACCCAAGAAC TGGGAATAAA TACAATGTTT ATCCGACATA TGATTTTGCC 
TGCCCCATAG TTGACAGCAT TGAAGGTGTT ACACACGCCC TGAGAACAAC AGAATACCAT 
GACAGAGATG AACAGTTCTA CTGGATTATT GAGGCATTGG GCATAAGAAA ACCATTTATT 
TGGGAATACA GTCGGCTGAA TCTCAACAAC ACAGTGCTGT CCAAAAGAAA ACTCACGTGG 
TTTGTCAATG AAGGACTAGT AGATGGATGG GATGACCCAC GGTTTCCTAC AGTTCGTGGT 
GTGCTGAGAA GAGGGATGAC GGTCGAAGGA CTAAAACAGT TTATTGCTGC TCAGGGTTCT 
TCACGTTCAG TTGTGAACAT GGAATGGGAC AAAATTTGGG CATTTAACAA AAAGGTTATT 
GACCCAGTGG CTCCACGATA CGTTGCATTA CTGAAGAAAG AGGTGGTCCC GGTGAACGTC 
CCCGAGGCCC AGGAGGAGAT GAAGGAAGTC GCCAAACACC CGAAGAATCC TGATGTTGGC 
TTGAAGCCTG TGTGGTATAG TTCTAAAGTT TTCATCGAAG GTGCTGATGC AGAGACATTG 
TCAGAGGGTG AGATGGTTAC ATTTATAAAC TGGGGCAACA TCAACATTAC TAAAATACAC 
AAAAATCCAG AAGGCAAAAT TGTATCTCTT GACGCAAAAT TGAATTTAGA GAACAAAGAC 
TACAAGAAAA CCACTAAGAT CACTTGGCTT GCAGAGACGG AACACGCGCT TCCTGTCCCA 
GCAATCTGTG TCACGTACGA GCACTTGATC ACGAAGCCCG TGCTGGGAAA AGATGAGGAC 
TTTAAGCAGT ATGTCAACAA AAACAGTAAG CATGAAGAAC TAATGCTGGG GGATCCCTGC 
CTTAAGGATT TGAAAAAAGG AGACATTATA CAACTCCAGA GAAGAGGCTT CTTCATATGT 
GATCAACCTT ATGAACCCGT TAGTCCATAT AGCTGCATAG AAGCCCCATG CATTCTGATA 
TACATTCCTG ATGGACATAC GAAGGAAATG CCAACATCTG GGTCAAAGGA GAAGACCAAA 
GTAGAAACAA CAAAGAATGA GGCATCTACA CCTCTCAAGA CAAGATCGGC TCCTTCTTTG 
AGTACTTGTG CTACATCTGA GGATTCCTTG GTCCTTTACA ACAGAGTTGC TGCCCAAGGG 
GACGTGGTTC GTGAATTAAA AGCCAAGAAA GCCACAAAGG AAGATATAGA TGCAGCTGTA 
AAACAGCTTT TGGCTTTGAA GGCTGAATAT AAGGAGAAAA CTGGCCAGGA ATATAAGCCT 
GGAAATCCTC CCGCTGCAGC AGGACAGAAT GTTTCTTCTA AATCATCATC AAATATTGTG 
GAAAGTAAAT CTCTGTATGA TGAAGTTGCC GCACAAGGAG AAGTGGTCCG TAAACTGAAA 
GCCGAGAAAG CCCCTAAGGC TAAAGTAAAT GAAGCTGTAG AATGCTTACT GTCCCTAAAA 
GCTCAGTATA AAGAAAAAAC TGGGAAGGAG TACGTTCCTG GTCAGCCCCC ATCATCTCAA 
AGTTCAGATT CAAGTCCACC CAGAAGCTCT GAGCCTAGTG GTCCAGAAAC ACCAGAAGCC 
AAACTACTTT TTGACAGAGT AGCTTCTCAA GGAGAAGTAG TTCGAAAACT TAAAGCTGAA 
AAAGCTTCTA AGGATCAAGT AGATACAGCC GTACAAGACC TCCTTCAGCT GAAAGCACAG 
TACAGGTCTC TGACAGGAGT AGACTATAAG CCTGTCTCTG CCACTGGGGC TGAGGAGAAA 
GATAAGAAAA AGAAAGAAAA GGAGAATAAA TCTGAAAAGC AGAATAAGCC TCAGAAGCAA 
AATGACACCC AAAAGAAAGA CTTCTCTAAA AACCAAGGAA GTGGGCTGTC ACCAAATGGA 
GCAGGGGAAG GACAAGGGCC TAAGAAGCAG ACCAGGTTGG GTCTTGAGGC CAGAAAAGAA 
GACAGTCTTG CAGATTGGTA TTCTCAAGTC ATCACAAAGT CAGAAATGAT TGAATACCAT 
GATGTAAGTG GCTGCTATAT CCTTCGTCCC TGGGCATATT CCATTTGGGA ATCCATCAAG 
GACTTTTTTG ATGCTGAGAT CAAGAAACTC GGTGTTGAAA ACTGCTATTT CCCCATGTTT 
GTGTCTCAAG GTGCATTAGA GAAAGAGAAG ACTCATATTG CTGACTTTGC TCCTGAGGTT 
GCTTGGGTGA CAAGATCTGG CAAAACAGAG TTGGCAGAAC CAATTGCCAT TCGTCCTACC 
AGTGAAACAG TAATGTATCC TGCATATGCG AAGTGGGTGC AGTCCCACAG AGACCTGCCC 
ATAAAGCTCA ACCAGTGGTG CAACGTGGTG CGTTGGGAGT TCAAGCACCC TCAGCCTTTC 
TTACGTACTC GTGAATTCCT CTGGCAGGAA GGGCACAGTG CCTTTGCTAC ATTTGAAGAG 
GCAGCAGAAG AGGTTTTGCA GATACTTGAC TTATACGCTC AGGTATATGA AGAACTCCTG 
GCAATTCCTG TTGTAAAGGG AAGAAAGACT GAAAAGGAGA AATTTGCGGG AGGAGATTAC 
ACCACTACAG TGGAAGCATT TATATCTGCC AGTGGAAGAG CTATCCAGGG AGCAACATCA 
CATCATTTAG GACAGAATTT CTCGAAAATG TTTGAAATCG TTTTTGAAGA TCCAAAGACA 
GCAGGAGAGA AGCAGTTGGC CTATCAGAAC TCCTGGGGCC TGACGACTCG AACTATCGGT 
GTGATGACCA TGGTTCACGG GGACAACGTG GGCTTAGTGC TGCCGCCCCG TGTAGCCTGT 
GTTCAGGTGG TGGTTATTCC CTGTGGCATC ACAAATGCAC TTTCTGAAGA AGACAGGGAT 
GCTCTACTTG CAAAATGCAA TGATTATAGA AGGCGGTTAC TCAGTGTCAA TATTCGAGTT 
CGAGTTGATT TGCGAGATAA TTACTCACCA GGTTGGAAAT TCAATCACTG GGAACTCAAG 
GGAGTCCCGA TTAGACTTGA AGTCGGGCCA CGTGATATGA AGAACTGCCA GTTTGTAGCT 
GTCAGACGAG ACACTGGGGA AAAGCTGACA ATTGCTGAAA ATGAGGCTGA GACGAAGCTT 
CGTGCTATTT TGGAAGACAT CCATGTTAAC CTCTTTACCC GGGCTTCTGA AGACCTTAAG 
ACTCATATGG TTGTGGCTAA TACAATGGAG GACTTCCAGA AGGAGCTCGA TTCTGGGAAG 
ATTGCTCAGA TTCCATTCTG TGGGGAAATT GACTGTGAAG ATTGGATCAA AAAGACTACT 
GCCAGGGATC AAGATCTTGA GCCCGGTGCT CCGTCGATGG GAGCTAAAAG CCTGTGCATT 
CCCTTCAAGC CACTCTGCGA GCTGCCGCCA GGAGCCAAGT GTGTGTGTGG AAAGAACCCT 
GCCAAGTACT ACACCTTGTT CGGTCGTAGT TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPRS ( XM_004013603.3 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_004013652.1 Ovis aries glutamyl-prolyl-tRNA synthetase (EPRS), transcript variant X2, mRNA.

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CloneID	OSm10564
Clone ID Related Accession (Same CDS sequence)	XM_004013603.3
Accession Version	XM_004013603.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	bifunctional glutamate/proline--tRNA ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639021.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_004013603.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGC TCTGTCTGAC TGTGAATTCG GGAGACCCTC CGCTAGGAGC TTTGTTGGCA 
GTGGAACATG TGAAAGACAA CGTCAGCATT TCTGTTGAAG AAGGGAAAGA GAATATTCTT 
CATGTATCTG AAAATGTGGT GTTCACGGAC ATAAACTCCA TACTTCGCTA CTTGGCTAGA 
ATTGCAACTA CAGCAGGGCT CTATGGCTCT AACTTGTTGG AACATACTGA GATTGATCAC 
TGGTTGGAGT TCAGTGCCAC GAAATTGTCT TCTTGTGATT TGTTTACTTC TGCAGTCAAC 
GAGCTCAATC ATTGTCTGTC TCTGAGAACA TACTTAGTTG GAAACTCCTT GAGCTTAGCA 
GATTTATGTG TTTGGGCCAC CCTAAAAGGA AATGCTGCGT GGCAGGAACA GTTGGGACAG 
AACAAAGCTC CAGTTCATGT AAAACGGTGG TTTGGCTTTC TTGAAGCCCA GCAGGCCTTC 
CAGTCAGTAG GTACCAAGTG GAATGTTTCA GCAACCAAAG CAAAAGTGGT TCCTGAGAAA 
AAGCAAGATG TTGGGAAATT TGTTGAGCTT CCAGGTGCTG AGATGGGGAA GGTTACAGTC 
AGATTTCCTC CAGAGGCTAG TGGTTACTTG CACATCGGGC ACGCAAAAGC TGCTCTCCTG 
AACCAGCACT ACCAGGTCAA CTTTAAAGGG AAACTGATCA TGAGATTCGA TGACACAAAT 
CCCGAAAAAG AAAAGGAAGA TTTTGAGAAG GTTATTTTGG AGGATGTTGC AATGTTGCAT 
ATCAAACCAG ATCAATTTAC TTACACCTCG GATCATTTTG AAACTATAAT GAAATATGCA 
GAAAAGCTAA TTCAGGAGGG GAAGGCTTAT GTCGACGATA CTCCTGCCGA GCAGATGAAA 
GCGGAGCGTG AGCAGAGGAT AGAATCCAAA CATAGAAAAA ACTCTGTGGA GAAGAATCTG 
CAAATGTGGG AAGAAATGAA AAAAGGAAGC CAGTTTGGTC AGTCCTGTTG TTTGCGAGCA 
AAAATTGACA TGAGTAGTAA CAATGGCTGC ATGAGGGACC CGACACTTTA TCGCTGCAAG 
ATTCAACCCC ACCCAAGAAC TGGGAATAAA TACAATGTTT ATCCGACATA TGATTTTGCC 
TGCCCCATAG TTGACAGCAT TGAAGGTGTT ACACATGCCC TGAGAACAAC AGAATACCAT 
GACAGAGATG AACAGTTCTA CTGGATTATT GAGGCGTTGG GCATAAGAAA ACCATTTATT 
TGGGAATACA GTCGGCTGAA TCTCAACAAC ACAGTGCTGT CCAAAAGAAA ACTCACGTGG 
TTTGTCAATG AAGGACTAGT AGATGGATGG GATGACCCAC GGTTTCCTAC AGTTCGTGGT 
GTGCTGAGAA GAGGGATGAC GGTCGAAGGA CTAAAACAGT TTATTGCTGC TCAGGGTTCT 
TCACGTTCAG TTGTGAACAT GGAATGGGAC AAAATTTGGG CATTTAACAA AAAGGTTATT 
GACCCAGTGG CTCCACGATA CGTTGCATTA CTGAAGAAAG AGGTGGTCCC AGTGAACGTC 
CCCGAGGCCC AGGAGGAGAT GAAGGAAGTC GCCAAACACC CGAAGAATCC TGATGTTGGC 
TTGAAGCCTG TGTGGTATAG TTCTAAAGTT TTCATCGAAG GTGCTGATGC AGAGACATTG 
TCAGAGGGTG AGATGGTTAC ATTTATAAAC TGGGGCAACA TCAACATTAC TAAAATACAC 
AAAAATGCAG AAGGCAAAAT TGTATCTCTT GACGCAAAAT TGAATTTAGA GAACAAAGAC 
TACAAGAAAA CCACTAAGAT CACTTGGCTT GCAGAGACGG AACACGCGCT TCCTGTCCCA 
GCAATCTGTG TCACGTACGA GCACTTGATC ACGAAGCCCG TGCTGGGAAA AGATGAGGAC 
TTTAAGCAGT ATGTCAACAA AAACAGTAAG CATGAAGAGC TAATGCTGGG GGATCCCTGC 
CTTAAGGATT TGAAAAAAGG AGACATTATA CAACTCCAGA GAAGAGGCTT CTTCATATGT 
GATCAACCTT ATGAACCCGT TAGTCCATAT AGCTGCATAG AAGCCCCATG CATTCTGATA 
TACATTCCTG ATGGACATAC GAAGGAAATG CCAACATCTG GGTCAAAGGA GAAGACCAAA 
GTAGAAACAA CAAAGAATGA GGCATCTACA CCTCTCAAGA CAAGATCGGC TCCTTCTTTG 
AGTACTTGTG CTACATCTGA GGATTCCTTG GTCCTTTACA ACAGAGTTGC TGCCCAAGGG 
GACGTGGTTC GTGAATTAAA AGCCAAGAAA GCCACAAAGG AAGATATAGA TGCAGCTGTA 
AAACAGCTTT TGGCTTTGAA GGCTGAATAT AAGGAGAAAA CTGGCCAGGA ATATAAGCCT 
GGAAATCCTC CCGCTGCAGC AGGACAGAAT GTTTCTTCTA AATCATCATC AAATATTGTG 
GAAAGTAAAT CTCTGTATGA TGAAGTTGCC GCACAAGGAG AAGTGGTCCG TAAACTGAAA 
GCCGAGAAAG CCCCTAAGGC TAAAGTAAAT GAAGCTGTAG AATGCTTACT GTCCCTAAAA 
GCTCAGTATA AAGAAAAAAC TGGGAAGGAG TACGTTCCTG GTCAGCCCCC ATCATCTCAA 
AGTTCAGATT CAAGTCCACC CAGAAGCTCT GAGCCTAGTG GTCCAGAAAC ACCAGAAGCC 
AAACTACTTT TTGACAGAGT AGCTTCTCAA GGAGAAGTAG TTCGAAAACT TAAAGCTGAA 
AAAGCTTCTA AGGATCAAGT AGATACAGCC GTACAAGACC TCCTTCAGCT GAAAGCACAG 
TACAGGTCTC TGACAGGAGT AGACTATAAG CCTGTCTCTG CCACTGGGGC TGAGGAGAAA 
GATAAGAAAA AGAAAGAAAA GGAGAATAAA TCTGAAAAGC AGAATAAGCC TCAGAAGCAA 
AATGACACCC AAAAGAAAGA CTTCTCTAAA AACCAAGGAA GTGGGCTGTC ACCAAATGGA 
GCAGGGGAAG GACAAGGGCC TAAGAAGCAG ACCAGGTTGG GTCTTGAGGC CAGAAAAGAA 
GACAGTCTTG CAGATTGGTA TTCTCAAGTC ATCACAAAGT CAGAAATGAT TGAATACCAT 
GATGTAAGTG GCTGCTATAT CCTTCGTCCC TGGGCATATT CCATTTGGGA ATCCATCAAG 
GACTTTTTTG ATGCTGAGAT CAAGAAACTC GGTGTTGAAA ACTGCTATTT CCCCATGTTT 
GTGTCTCAAG GTGCATTAGA GAAAGAGAAG ACTCATATTG CTGACTTTGC TCCTGAGGTT 
GCTTGGGTGA CAAGATCTGG CAAAACAGAG TTGGCAGAAC CAATTGCCAT TCGTCCTACC 
AGTGAAACAG TAATGTATCC TGCATATGCG AAGTGGGTGC AGTCCCACAG AGACCTGCCC 
ATAAAGCTCA ACCAGTGGTG CAACGTGGTG CGTTGGGAGT TCAAGCACCC TCAGCCTTTC 
TTACGTACTC GTGAATTCCT CTGGCAGGAA GGGCACAGTG CCTTTGCTAC ATTTGAAGAG 
GCAGCAGAAG AGGTTTTGCA GATACTTGAC TTATACGCTC AGGTATATGA AGAACTCCTG 
GCAATTCCTG TTGTAAAGGG AAGAAAGACT GAAAAGGAGA AATTTGCGGG AGGAGATTAC 
ACCACTACAG TGGAAGCATT TATATCTGCC AGTGGAAGAG CTATCCAGGG AGCAACATCA 
CATCATTTAG GACAGAATTT CTCGAAAATG TTTGAAATCG TTTTTGAAGA TCCAAAGACA 
GCAGGAGAGA AGCAGTTGGC CTATCAGAAC TCCTGGGGCC TGACGACTCG AACTATCGGT 
GTGATGACCA TGGTTCACGG GGACAACGTG GGCTTAGTGC TGCCGCCCCG TGTAGCCTGT 
GTTCAGGTGG TGGTTATTCC CTGTGGCATC ACAAATGCAC TTTCTGAAGA AGACAGGGAT 
GCTCTACTTG CAAAATGCAA TGATTATAGA AGGCGGTTAC TCAGTGTCAA TATTCGAGTT 
CGAGTTGATT TGCGAGATAA TTACTCACCA GGTTGGAAAT TCAATCACTG GGAACTCAAG 
GGAGTCCCGA TTAGACTTGA AGTTGGGCCA CGTGATATGA AGAACTGCCA GTTTGTAGCT 
GTCAGACGAG ACACTGGGGA AAAGCTGACA ATTGCTGAAA ATGAGGCTGA GACGAAGCTT 
CGTGCTATTT TGGAAGACAT CCACACTAAC CTCTTTACCC GGGCTTCTGA AGACCTTAAG 
ACTCATATGG TTGTGGCTAA TACAATGGAG GACTTCCAGA AGGAGCTCGA TTCTGGGAAG 
ATTGCTCAGA TTCCATTCTG TGGGGAAATT GACTGTGAAG ATTGGATCAA AAAGACTACT 
GCCAGGGATC AAGATCTTGA GCCCGGTGCT CCGTCGATGG GAGCTAAAAG CCTGTGCATT 
CCCTTCAAGC CACTCTGCGA GCTGCCGCCA GGAGCCAAGT GTGTGTGTGG AAAGAACCCT 
GCCAAGTACT ACACCTTGTT CGGTCGTAGT TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004013652.1
CDS	139..4674
Translation	MATLCLTVNSGDPPLGALLAVEHVKDNVSISVEEGKENILHVSENVVFTDINSILRYLARIATTAGLYGSNLLEHTEIDHWLEFSATKLSSCDLFTSAVNELNHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLGQNKAPVHVKRWFGFLEAQQAFQSVGTKWNVSATKAKVVPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRIESKHRKNSVEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPFIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVVPVNVPEAQEEMKEVAKHPKNPDVGLKPVWYSSKVFIEGADAETLSEGEMVTFINWGNINITKIHKNAEGKIVSLDAKLNLENKDYKKTTKITWLAETEHALPVPAICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCIEAPCILIYIPDGHTKEMPTSGSKEKTKVETTKNEASTPLKTRSAPSLSTCATSEDSLVLYNRVAAQGDVVRELKAKKATKEDIDAAVKQLLALKAEYKEKTGQEYKPGNPPAAAGQNVSSKSSSNIVESKSLYDEVAAQGEVVRKLKAEKAPKAKVNEAVECLLSLKAQYKEKTGKEYVPGQPPSSQSSDSSPPRSSEPSGPETPEAKLLFDRVASQGEVVRKLKAEKASKDQVDTAVQDLLQLKAQYRSLTGVDYKPVSATGAEEKDKKKKEKENKSEKQNKPQKQNDTQKKDFSKNQGSGLSPNGAGEGQGPKKQTRLGLEARKEDSLADWYSQVITKSEMIEYHDVSGCYILRPWAYSIWESIKDFFDAEIKKLGVENCYFPMFVSQGALEKEKTHIADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATFEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTVEAFISASGRAIQGATSHHLGQNFSKMFEIVFEDPKTAGEKQLAYQNSWGLTTRTIGVMTMVHGDNVGLVLPPRVACVQVVVIPCGITNALSEEDRDALLAKCNDYRRRLLSVNIRVRVDLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKNCQFVAVRRDTGEKLTIAENEAETKLRAILEDIHTNLFTRASEDLKTHMVVANTMEDFQKELDSGKIAQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELPPGAKCVCGKNPAKYYTLFGRSY

Target ORF information:

RefSeq Version	XM_004013603.3
Organism	Ovis aries(sheep)
Definition	Ovis aries glutamyl-prolyl-tRNA synthetase (EPRS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004013603.3

ORF Insert Sequence:

ATGGCGACGC TCTGTCTGAC TGTGAATTCG GGAGACCCTC CGCTAGGAGC TTTGTTGGCA 
GTGGAACATG TGAAAGACAA CGTCAGCATT TCTGTTGAAG AAGGGAAAGA GAATATTCTT 
CATGTATCTG AAAATGTGGT GTTCACGGAC ATAAACTCCA TACTTCGCTA CTTGGCTAGA 
ATTGCAACTA CAGCAGGGCT CTATGGCTCT AACTTGTTGG AACATACTGA GATTGATCAC 
TGGTTGGAGT TCAGTGCCAC GAAATTGTCT TCTTGTGATT TGTTTACTTC TGCAGTCAAC 
GAGCTCAATC ATTGTCTGTC TCTGAGAACA TACTTAGTTG GAAACTCCTT GAGCTTAGCA 
GATTTATGTG TTTGGGCCAC CCTAAAAGGA AATGCTGCGT GGCAGGAACA GTTGGGACAG 
AACAAAGCTC CAGTTCATGT AAAACGGTGG TTTGGCTTTC TTGAAGCCCA GCAGGCCTTC 
CAGTCAGTAG GTACCAAGTG GAATGTTTCA GCAACCAAAG CAAAAGTGGT TCCTGAGAAA 
AAGCAAGATG TTGGGAAATT TGTTGAGCTT CCAGGTGCTG AGATGGGGAA GGTTACAGTC 
AGATTTCCTC CAGAGGCTAG TGGTTACTTG CACATCGGGC ACGCAAAAGC TGCTCTCCTG 
AACCAGCACT ACCAGGTCAA CTTTAAAGGG AAACTGATCA TGAGATTCGA TGACACAAAT 
CCCGAAAAAG AAAAGGAAGA TTTTGAGAAG GTTATTTTGG AGGATGTTGC AATGTTGCAT 
ATCAAACCAG ATCAATTTAC TTACACCTCG GATCATTTTG AAACTATAAT GAAATATGCA 
GAAAAGCTAA TTCAGGAGGG GAAGGCTTAT GTCGACGATA CTCCTGCCGA GCAGATGAAA 
GCGGAGCGTG AGCAGAGGAT AGAATCCAAA CATAGAAAAA ACTCTGTGGA GAAGAATCTG 
CAAATGTGGG AAGAAATGAA AAAAGGAAGC CAGTTTGGTC AGTCCTGTTG TTTGCGAGCA 
AAAATTGACA TGAGTAGTAA CAATGGCTGC ATGAGGGACC CGACACTTTA TCGCTGCAAG 
ATTCAACCCC ACCCAAGAAC TGGGAATAAA TACAATGTTT ATCCGACATA TGATTTTGCC 
TGCCCCATAG TTGACAGCAT TGAAGGTGTT ACACATGCCC TGAGAACAAC AGAATACCAT 
GACAGAGATG AACAGTTCTA CTGGATTATT GAGGCGTTGG GCATAAGAAA ACCATTTATT 
TGGGAATACA GTCGGCTGAA TCTCAACAAC ACAGTGCTGT CCAAAAGAAA ACTCACGTGG 
TTTGTCAATG AAGGACTAGT AGATGGATGG GATGACCCAC GGTTTCCTAC AGTTCGTGGT 
GTGCTGAGAA GAGGGATGAC GGTCGAAGGA CTAAAACAGT TTATTGCTGC TCAGGGTTCT 
TCACGTTCAG TTGTGAACAT GGAATGGGAC AAAATTTGGG CATTTAACAA AAAGGTTATT 
GACCCAGTGG CTCCACGATA CGTTGCATTA CTGAAGAAAG AGGTGGTCCC AGTGAACGTC 
CCCGAGGCCC AGGAGGAGAT GAAGGAAGTC GCCAAACACC CGAAGAATCC TGATGTTGGC 
TTGAAGCCTG TGTGGTATAG TTCTAAAGTT TTCATCGAAG GTGCTGATGC AGAGACATTG 
TCAGAGGGTG AGATGGTTAC ATTTATAAAC TGGGGCAACA TCAACATTAC TAAAATACAC 
AAAAATGCAG AAGGCAAAAT TGTATCTCTT GACGCAAAAT TGAATTTAGA GAACAAAGAC 
TACAAGAAAA CCACTAAGAT CACTTGGCTT GCAGAGACGG AACACGCGCT TCCTGTCCCA 
GCAATCTGTG TCACGTACGA GCACTTGATC ACGAAGCCCG TGCTGGGAAA AGATGAGGAC 
TTTAAGCAGT ATGTCAACAA AAACAGTAAG CATGAAGAGC TAATGCTGGG GGATCCCTGC 
CTTAAGGATT TGAAAAAAGG AGACATTATA CAACTCCAGA GAAGAGGCTT CTTCATATGT 
GATCAACCTT ATGAACCCGT TAGTCCATAT AGCTGCATAG AAGCCCCATG CATTCTGATA 
TACATTCCTG ATGGACATAC GAAGGAAATG CCAACATCTG GGTCAAAGGA GAAGACCAAA 
GTAGAAACAA CAAAGAATGA GGCATCTACA CCTCTCAAGA CAAGATCGGC TCCTTCTTTG 
AGTACTTGTG CTACATCTGA GGATTCCTTG GTCCTTTACA ACAGAGTTGC TGCCCAAGGG 
GACGTGGTTC GTGAATTAAA AGCCAAGAAA GCCACAAAGG AAGATATAGA TGCAGCTGTA 
AAACAGCTTT TGGCTTTGAA GGCTGAATAT AAGGAGAAAA CTGGCCAGGA ATATAAGCCT 
GGAAATCCTC CCGCTGCAGC AGGACAGAAT GTTTCTTCTA AATCATCATC AAATATTGTG 
GAAAGTAAAT CTCTGTATGA TGAAGTTGCC GCACAAGGAG AAGTGGTCCG TAAACTGAAA 
GCCGAGAAAG CCCCTAAGGC TAAAGTAAAT GAAGCTGTAG AATGCTTACT GTCCCTAAAA 
GCTCAGTATA AAGAAAAAAC TGGGAAGGAG TACGTTCCTG GTCAGCCCCC ATCATCTCAA 
AGTTCAGATT CAAGTCCACC CAGAAGCTCT GAGCCTAGTG GTCCAGAAAC ACCAGAAGCC 
AAACTACTTT TTGACAGAGT AGCTTCTCAA GGAGAAGTAG TTCGAAAACT TAAAGCTGAA 
AAAGCTTCTA AGGATCAAGT AGATACAGCC GTACAAGACC TCCTTCAGCT GAAAGCACAG 
TACAGGTCTC TGACAGGAGT AGACTATAAG CCTGTCTCTG CCACTGGGGC TGAGGAGAAA 
GATAAGAAAA AGAAAGAAAA GGAGAATAAA TCTGAAAAGC AGAATAAGCC TCAGAAGCAA 
AATGACACCC AAAAGAAAGA CTTCTCTAAA AACCAAGGAA GTGGGCTGTC ACCAAATGGA 
GCAGGGGAAG GACAAGGGCC TAAGAAGCAG ACCAGGTTGG GTCTTGAGGC CAGAAAAGAA 
GACAGTCTTG CAGATTGGTA TTCTCAAGTC ATCACAAAGT CAGAAATGAT TGAATACCAT 
GATGTAAGTG GCTGCTATAT CCTTCGTCCC TGGGCATATT CCATTTGGGA ATCCATCAAG 
GACTTTTTTG ATGCTGAGAT CAAGAAACTC GGTGTTGAAA ACTGCTATTT CCCCATGTTT 
GTGTCTCAAG GTGCATTAGA GAAAGAGAAG ACTCATATTG CTGACTTTGC TCCTGAGGTT 
GCTTGGGTGA CAAGATCTGG CAAAACAGAG TTGGCAGAAC CAATTGCCAT TCGTCCTACC 
AGTGAAACAG TAATGTATCC TGCATATGCG AAGTGGGTGC AGTCCCACAG AGACCTGCCC 
ATAAAGCTCA ACCAGTGGTG CAACGTGGTG CGTTGGGAGT TCAAGCACCC TCAGCCTTTC 
TTACGTACTC GTGAATTCCT CTGGCAGGAA GGGCACAGTG CCTTTGCTAC ATTTGAAGAG 
GCAGCAGAAG AGGTTTTGCA GATACTTGAC TTATACGCTC AGGTATATGA AGAACTCCTG 
GCAATTCCTG TTGTAAAGGG AAGAAAGACT GAAAAGGAGA AATTTGCGGG AGGAGATTAC 
ACCACTACAG TGGAAGCATT TATATCTGCC AGTGGAAGAG CTATCCAGGG AGCAACATCA 
CATCATTTAG GACAGAATTT CTCGAAAATG TTTGAAATCG TTTTTGAAGA TCCAAAGACA 
GCAGGAGAGA AGCAGTTGGC CTATCAGAAC TCCTGGGGCC TGACGACTCG AACTATCGGT 
GTGATGACCA TGGTTCACGG GGACAACGTG GGCTTAGTGC TGCCGCCCCG TGTAGCCTGT 
GTTCAGGTGG TGGTTATTCC CTGTGGCATC ACAAATGCAC TTTCTGAAGA AGACAGGGAT 
GCTCTACTTG CAAAATGCAA TGATTATAGA AGGCGGTTAC TCAGTGTCAA TATTCGAGTT 
CGAGTTGATT TGCGAGATAA TTACTCACCA GGTTGGAAAT TCAATCACTG GGAACTCAAG 
GGAGTCCCGA TTAGACTTGA AGTTGGGCCA CGTGATATGA AGAACTGCCA GTTTGTAGCT 
GTCAGACGAG ACACTGGGGA AAAGCTGACA ATTGCTGAAA ATGAGGCTGA GACGAAGCTT 
CGTGCTATTT TGGAAGACAT CCACACTAAC CTCTTTACCC GGGCTTCTGA AGACCTTAAG 
ACTCATATGG TTGTGGCTAA TACAATGGAG GACTTCCAGA AGGAGCTCGA TTCTGGGAAG 
ATTGCTCAGA TTCCATTCTG TGGGGAAATT GACTGTGAAG ATTGGATCAA AAAGACTACT 
GCCAGGGATC AAGATCTTGA GCCCGGTGCT CCGTCGATGG GAGCTAAAAG CCTGTGCATT 
CCCTTCAAGC CACTCTGCGA GCTGCCGCCA GGAGCCAAGT GTGTGTGTGG AAAGAACCCT 
GCCAAGTACT ACACCTTGTT CGGTCGTAGT TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPRS ( XM_012187055.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012042445.1 Ovis aries glutamyl-prolyl-tRNA synthetase (EPRS), transcript variant X1, mRNA.

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CloneID	OSm25588
Clone ID Related Accession (Same CDS sequence)	XM_012187055.2
Accession Version	XM_012187055.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	bifunctional glutamate/proline--tRNA ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639021.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012187055.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGC TCTGTCTGAC TGTGAATTCG GGAGACCCTC CGCTAGGAGC TTTGTTGGCA 
GTGGAACATG TGAAAGACAA CGTCAGCATT TCTGTTGAAG AAGGGAAAGA GAATATTCTT 
CATGTATCTG AAAATGTGGT GTTCACGGAC ATAAACTCCA TACTTCGCTA CTTGGCTAGA 
ATTGCAACTA CAGCAGGGCT CTATGGCTCT AACTTGTTGG AACATACTGA GATTGATCAC 
TGGTTGGAGT TCAGTGCCAC GAAATTGTCT TCTTGTGATT TGTTTACTTC TGCAGTCAAC 
GAGCTCAATC ATTGTCTGTC TCTGAGAACA TACTTAGTTG GAAACTCCTT GAGCTTAGCA 
GATTTATGTG TTTGGGCCAC CCTAAAAGGA AATGCTGCGT GGCAGGAACA GTTGGGACAG 
AACAAAGCTC CAGTTCATGT AAAACGGTGG TTTGGCTTTC TTGAAGCCCA GCAGGCCTTC 
CAGTCAGTAG GTACCAAGTG GAATGTTTCA GCAACCAAAG CAAAAGTGGT TCCTGAGAAA 
AAGCAAGATG TTGGGAAATT TGTTGAGCTT CCAGGTGCTG AGATGGGGAA GGTTACAGTC 
AGATTTCCTC CAGAGGCTAG TGGTTACTTG CACATCGGGC ACGCAAAAGC TGCTCTCCTG 
AACCAGCACT ACCAGGTCAA CTTTAAAGGG AAACTGATCA TGAGATTCGA TGACACAAAT 
CCCGAAAAAG AAAAGGAAGA TTTTGAGAAG GTTATTTTGG AGGATGTTGC AATGTTGCAT 
ATCAAACCAG ATCAATTTAC TTACACCTCG GATCATTTTG AAACTATAAT GAAATATGCA 
GAAAAGCTAA TTCAGGAGGG GAAGGCTTAT GTCGACGATA CTCCTGCCGA GCAGATGAAA 
GCGGAGCGTG AGCAGAGGAT AGAATCCAAA CATAGAAAAA ACTCTGTGGA GAAGAATCTG 
CAAATGTGGG AAGAAATGAA AAAAGGAAGC CAGTTTGGTC AGTCCTGTTG TTTGCGAGCA 
AAAATTGACA TGAGTAGTAA CAATGGCTGC ATGAGGGACC CGACACTTTA TCGCTGCAAG 
ATTCAACCCC ACCCAAGAAC TGGGAATAAA TACAATGTTT ATCCGACATA TGATTTTGCC 
TGCCCCATAG TTGACAGCAT TGAAGGTGTT ACACATGCCC TGAGAACAAC AGAATACCAT 
GACAGAGATG AACAGTTCTA CTGGATTATT GAGGCGTTGG GCATAAGAAA ACCATTTATT 
TGGGAATACA GTCGGCTGAA TCTCAACAAC ACAGTGCTGT CCAAAAGAAA ACTCACGTGG 
TTTGTCAATG AAGGACTAGT AGATGGATGG GATGACCCAC GGTTTCCTAC AGTTCGTGGT 
GTGCTGAGAA GAGGGATGAC GGTCGAAGGA CTAAAACAGT TTATTGCTGC TCAGGGTTCT 
TCACGTTCAG TTGTGAACAT GGAATGGGAC AAAATTTGGG CATTTAACAA AAAGCTGCGA 
GCTCTCTGTA AGAAGGTTAT TGACCCAGTG GCTCCACGAT ACGTTGCATT ACTGAAGAAA 
GAGGTGGTCC CAGTGAACGT CCCCGAGGCC CAGGAGGAGA TGAAGGAAGT CGCCAAACAC 
CCGAAGAATC CTGATGTTGG CTTGAAGCCT GTGTGGTATA GTTCTAAAGT TTTCATCGAA 
GGTGCTGATG CAGAGACATT GTCAGAGGGT GAGATGGTTA CATTTATAAA CTGGGGCAAC 
ATCAACATTA CTAAAATACA CAAAAATGCA GAAGGCAAAA TTGTATCTCT TGACGCAAAA 
TTGAATTTAG AGAACAAAGA CTACAAGAAA ACCACTAAGA TCACTTGGCT TGCAGAGACG 
GAACACGCGC TTCCTGTCCC AGCAATCTGT GTCACGTACG AGCACTTGAT CACGAAGCCC 
GTGCTGGGAA AAGATGAGGA CTTTAAGCAG TATGTCAACA AAAACAGTAA GCATGAAGAG 
CTAATGCTGG GGGATCCCTG CCTTAAGGAT TTGAAAAAAG GAGACATTAT ACAACTCCAG 
AGAAGAGGCT TCTTCATATG TGATCAACCT TATGAACCCG TTAGTCCATA TAGCTGCATA 
GAAGCCCCAT GCATTCTGAT ATACATTCCT GATGGACATA CGAAGGAAAT GCCAACATCT 
GGGTCAAAGG AGAAGACCAA AGTAGAAACA ACAAAGAATG AGGCATCTAC ACCTCTCAAG 
ACAAGATCGG CTCCTTCTTT GAGTACTTGT GCTACATCTG AGGATTCCTT GGTCCTTTAC 
AACAGAGTTG CTGCCCAAGG GGACGTGGTT CGTGAATTAA AAGCCAAGAA AGCCACAAAG 
GAAGATATAG ATGCAGCTGT AAAACAGCTT TTGGCTTTGA AGGCTGAATA TAAGGAGAAA 
ACTGGCCAGG AATATAAGCC TGGAAATCCT CCCGCTGCAG CAGGACAGAA TGTTTCTTCT 
AAATCATCAT CAAATATTGT GGAAAGTAAA TCTCTGTATG ATGAAGTTGC CGCACAAGGA 
GAAGTGGTCC GTAAACTGAA AGCCGAGAAA GCCCCTAAGG CTAAAGTAAA TGAAGCTGTA 
GAATGCTTAC TGTCCCTAAA AGCTCAGTAT AAAGAAAAAA CTGGGAAGGA GTACGTTCCT 
GGTCAGCCCC CATCATCTCA AAGTTCAGAT TCAAGTCCAC CCAGAAGCTC TGAGCCTAGT 
GGTCCAGAAA CACCAGAAGC CAAACTACTT TTTGACAGAG TAGCTTCTCA AGGAGAAGTA 
GTTCGAAAAC TTAAAGCTGA AAAAGCTTCT AAGGATCAAG TAGATACAGC CGTACAAGAC 
CTCCTTCAGC TGAAAGCACA GTACAGGTCT CTGACAGGAG TAGACTATAA GCCTGTCTCT 
GCCACTGGGG CTGAGGAGAA AGATAAGAAA AAGAAAGAAA AGGAGAATAA ATCTGAAAAG 
CAGAATAAGC CTCAGAAGCA AAATGACACC CAAAAGAAAG ACTTCTCTAA AAACCAAGGA 
AGTGGGCTGT CACCAAATGG AGCAGGGGAA GGACAAGGGC CTAAGAAGCA GACCAGGTTG 
GGTCTTGAGG CCAGAAAAGA AGACAGTCTT GCAGATTGGT ATTCTCAAGT CATCACAAAG 
TCAGAAATGA TTGAATACCA TGATGTAAGT GGCTGCTATA TCCTTCGTCC CTGGGCATAT 
TCCATTTGGG AATCCATCAA GGACTTTTTT GATGCTGAGA TCAAGAAACT CGGTGTTGAA 
AACTGCTATT TCCCCATGTT TGTGTCTCAA GGTGCATTAG AGAAAGAGAA GACTCATATT 
GCTGACTTTG CTCCTGAGGT TGCTTGGGTG ACAAGATCTG GCAAAACAGA GTTGGCAGAA 
CCAATTGCCA TTCGTCCTAC CAGTGAAACA GTAATGTATC CTGCATATGC GAAGTGGGTG 
CAGTCCCACA GAGACCTGCC CATAAAGCTC AACCAGTGGT GCAACGTGGT GCGTTGGGAG 
TTCAAGCACC CTCAGCCTTT CTTACGTACT CGTGAATTCC TCTGGCAGGA AGGGCACAGT 
GCCTTTGCTA CATTTGAAGA GGCAGCAGAA GAGGTTTTGC AGATACTTGA CTTATACGCT 
CAGGTATATG AAGAACTCCT GGCAATTCCT GTTGTAAAGG GAAGAAAGAC TGAAAAGGAG 
AAATTTGCGG GAGGAGATTA CACCACTACA GTGGAAGCAT TTATATCTGC CAGTGGAAGA 
GCTATCCAGG GAGCAACATC ACATCATTTA GGACAGAATT TCTCGAAAAT GTTTGAAATC 
GTTTTTGAAG ATCCAAAGAC AGCAGGAGAG AAGCAGTTGG CCTATCAGAA CTCCTGGGGC 
CTGACGACTC GAACTATCGG TGTGATGACC ATGGTTCACG GGGACAACGT GGGCTTAGTG 
CTGCCGCCCC GTGTAGCCTG TGTTCAGGTG GTGGTTATTC CCTGTGGCAT CACAAATGCA 
CTTTCTGAAG AAGACAGGGA TGCTCTACTT GCAAAATGCA ATGATTATAG AAGGCGGTTA 
CTCAGTGTCA ATATTCGAGT TCGAGTTGAT TTGCGAGATA ATTACTCACC AGGTTGGAAA 
TTCAATCACT GGGAACTCAA GGGAGTCCCG ATTAGACTTG AAGTTGGGCC ACGTGATATG 
AAGAACTGCC AGTTTGTAGC TGTCAGACGA GACACTGGGG AAAAGCTGAC AATTGCTGAA 
AATGAGGCTG AGACGAAGCT TCGTGCTATT TTGGAAGACA TCCACACTAA CCTCTTTACC 
CGGGCTTCTG AAGACCTTAA GACTCATATG GTTGTGGCTA ATACAATGGA GGACTTCCAG 
AAGGAGCTCG ATTCTGGGAA GATTGCTCAG ATTCCATTCT GTGGGGAAAT TGACTGTGAA 
GATTGGATCA AAAAGACTAC TGCCAGGGAT CAAGATCTTG AGCCCGGTGC TCCGTCGATG 
GGAGCTAAAA GCCTGTGCAT TCCCTTCAAG CCACTCTGCG AGCTGCCGCC AGGAGCCAAG 
TGTGTGTGTG GAAAGAACCC TGCCAAGTAC TACACCTTGT TCGGTCGTAG TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012042445.1
CDS	139..4695
Translation	MATLCLTVNSGDPPLGALLAVEHVKDNVSISVEEGKENILHVSENVVFTDINSILRYLARIATTAGLYGSNLLEHTEIDHWLEFSATKLSSCDLFTSAVNELNHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLGQNKAPVHVKRWFGFLEAQQAFQSVGTKWNVSATKAKVVPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRIESKHRKNSVEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPFIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKLRALCKKVIDPVAPRYVALLKKEVVPVNVPEAQEEMKEVAKHPKNPDVGLKPVWYSSKVFIEGADAETLSEGEMVTFINWGNINITKIHKNAEGKIVSLDAKLNLENKDYKKTTKITWLAETEHALPVPAICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCIEAPCILIYIPDGHTKEMPTSGSKEKTKVETTKNEASTPLKTRSAPSLSTCATSEDSLVLYNRVAAQGDVVRELKAKKATKEDIDAAVKQLLALKAEYKEKTGQEYKPGNPPAAAGQNVSSKSSSNIVESKSLYDEVAAQGEVVRKLKAEKAPKAKVNEAVECLLSLKAQYKEKTGKEYVPGQPPSSQSSDSSPPRSSEPSGPETPEAKLLFDRVASQGEVVRKLKAEKASKDQVDTAVQDLLQLKAQYRSLTGVDYKPVSATGAEEKDKKKKEKENKSEKQNKPQKQNDTQKKDFSKNQGSGLSPNGAGEGQGPKKQTRLGLEARKEDSLADWYSQVITKSEMIEYHDVSGCYILRPWAYSIWESIKDFFDAEIKKLGVENCYFPMFVSQGALEKEKTHIADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATFEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTVEAFISASGRAIQGATSHHLGQNFSKMFEIVFEDPKTAGEKQLAYQNSWGLTTRTIGVMTMVHGDNVGLVLPPRVACVQVVVIPCGITNALSEEDRDALLAKCNDYRRRLLSVNIRVRVDLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKNCQFVAVRRDTGEKLTIAENEAETKLRAILEDIHTNLFTRASEDLKTHMVVANTMEDFQKELDSGKIAQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELPPGAKCVCGKNPAKYYTLFGRSY

Target ORF information:

RefSeq Version	XM_012187055.2
Organism	Ovis aries(sheep)
Definition	Ovis aries glutamyl-prolyl-tRNA synthetase (EPRS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012187055.2

ORF Insert Sequence:

ATGGCGACGC TCTGTCTGAC TGTGAATTCG GGAGACCCTC CGCTAGGAGC TTTGTTGGCA 
GTGGAACATG TGAAAGACAA CGTCAGCATT TCTGTTGAAG AAGGGAAAGA GAATATTCTT 
CATGTATCTG AAAATGTGGT GTTCACGGAC ATAAACTCCA TACTTCGCTA CTTGGCTAGA 
ATTGCAACTA CAGCAGGGCT CTATGGCTCT AACTTGTTGG AACATACTGA GATTGATCAC 
TGGTTGGAGT TCAGTGCCAC GAAATTGTCT TCTTGTGATT TGTTTACTTC TGCAGTCAAC 
GAGCTCAATC ATTGTCTGTC TCTGAGAACA TACTTAGTTG GAAACTCCTT GAGCTTAGCA 
GATTTATGTG TTTGGGCCAC CCTAAAAGGA AATGCTGCGT GGCAGGAACA GTTGGGACAG 
AACAAAGCTC CAGTTCATGT AAAACGGTGG TTTGGCTTTC TTGAAGCCCA GCAGGCCTTC 
CAGTCAGTAG GTACCAAGTG GAATGTTTCA GCAACCAAAG CAAAAGTGGT TCCTGAGAAA 
AAGCAAGATG TTGGGAAATT TGTTGAGCTT CCAGGTGCTG AGATGGGGAA GGTTACAGTC 
AGATTTCCTC CAGAGGCTAG TGGTTACTTG CACATCGGGC ACGCAAAAGC TGCTCTCCTG 
AACCAGCACT ACCAGGTCAA CTTTAAAGGG AAACTGATCA TGAGATTCGA TGACACAAAT 
CCCGAAAAAG AAAAGGAAGA TTTTGAGAAG GTTATTTTGG AGGATGTTGC AATGTTGCAT 
ATCAAACCAG ATCAATTTAC TTACACCTCG GATCATTTTG AAACTATAAT GAAATATGCA 
GAAAAGCTAA TTCAGGAGGG GAAGGCTTAT GTCGACGATA CTCCTGCCGA GCAGATGAAA 
GCGGAGCGTG AGCAGAGGAT AGAATCCAAA CATAGAAAAA ACTCTGTGGA GAAGAATCTG 
CAAATGTGGG AAGAAATGAA AAAAGGAAGC CAGTTTGGTC AGTCCTGTTG TTTGCGAGCA 
AAAATTGACA TGAGTAGTAA CAATGGCTGC ATGAGGGACC CGACACTTTA TCGCTGCAAG 
ATTCAACCCC ACCCAAGAAC TGGGAATAAA TACAATGTTT ATCCGACATA TGATTTTGCC 
TGCCCCATAG TTGACAGCAT TGAAGGTGTT ACACATGCCC TGAGAACAAC AGAATACCAT 
GACAGAGATG AACAGTTCTA CTGGATTATT GAGGCGTTGG GCATAAGAAA ACCATTTATT 
TGGGAATACA GTCGGCTGAA TCTCAACAAC ACAGTGCTGT CCAAAAGAAA ACTCACGTGG 
TTTGTCAATG AAGGACTAGT AGATGGATGG GATGACCCAC GGTTTCCTAC AGTTCGTGGT 
GTGCTGAGAA GAGGGATGAC GGTCGAAGGA CTAAAACAGT TTATTGCTGC TCAGGGTTCT 
TCACGTTCAG TTGTGAACAT GGAATGGGAC AAAATTTGGG CATTTAACAA AAAGCTGCGA 
GCTCTCTGTA AGAAGGTTAT TGACCCAGTG GCTCCACGAT ACGTTGCATT ACTGAAGAAA 
GAGGTGGTCC CAGTGAACGT CCCCGAGGCC CAGGAGGAGA TGAAGGAAGT CGCCAAACAC 
CCGAAGAATC CTGATGTTGG CTTGAAGCCT GTGTGGTATA GTTCTAAAGT TTTCATCGAA 
GGTGCTGATG CAGAGACATT GTCAGAGGGT GAGATGGTTA CATTTATAAA CTGGGGCAAC 
ATCAACATTA CTAAAATACA CAAAAATGCA GAAGGCAAAA TTGTATCTCT TGACGCAAAA 
TTGAATTTAG AGAACAAAGA CTACAAGAAA ACCACTAAGA TCACTTGGCT TGCAGAGACG 
GAACACGCGC TTCCTGTCCC AGCAATCTGT GTCACGTACG AGCACTTGAT CACGAAGCCC 
GTGCTGGGAA AAGATGAGGA CTTTAAGCAG TATGTCAACA AAAACAGTAA GCATGAAGAG 
CTAATGCTGG GGGATCCCTG CCTTAAGGAT TTGAAAAAAG GAGACATTAT ACAACTCCAG 
AGAAGAGGCT TCTTCATATG TGATCAACCT TATGAACCCG TTAGTCCATA TAGCTGCATA 
GAAGCCCCAT GCATTCTGAT ATACATTCCT GATGGACATA CGAAGGAAAT GCCAACATCT 
GGGTCAAAGG AGAAGACCAA AGTAGAAACA ACAAAGAATG AGGCATCTAC ACCTCTCAAG 
ACAAGATCGG CTCCTTCTTT GAGTACTTGT GCTACATCTG AGGATTCCTT GGTCCTTTAC 
AACAGAGTTG CTGCCCAAGG GGACGTGGTT CGTGAATTAA AAGCCAAGAA AGCCACAAAG 
GAAGATATAG ATGCAGCTGT AAAACAGCTT TTGGCTTTGA AGGCTGAATA TAAGGAGAAA 
ACTGGCCAGG AATATAAGCC TGGAAATCCT CCCGCTGCAG CAGGACAGAA TGTTTCTTCT 
AAATCATCAT CAAATATTGT GGAAAGTAAA TCTCTGTATG ATGAAGTTGC CGCACAAGGA 
GAAGTGGTCC GTAAACTGAA AGCCGAGAAA GCCCCTAAGG CTAAAGTAAA TGAAGCTGTA 
GAATGCTTAC TGTCCCTAAA AGCTCAGTAT AAAGAAAAAA CTGGGAAGGA GTACGTTCCT 
GGTCAGCCCC CATCATCTCA AAGTTCAGAT TCAAGTCCAC CCAGAAGCTC TGAGCCTAGT 
GGTCCAGAAA CACCAGAAGC CAAACTACTT TTTGACAGAG TAGCTTCTCA AGGAGAAGTA 
GTTCGAAAAC TTAAAGCTGA AAAAGCTTCT AAGGATCAAG TAGATACAGC CGTACAAGAC 
CTCCTTCAGC TGAAAGCACA GTACAGGTCT CTGACAGGAG TAGACTATAA GCCTGTCTCT 
GCCACTGGGG CTGAGGAGAA AGATAAGAAA AAGAAAGAAA AGGAGAATAA ATCTGAAAAG 
CAGAATAAGC CTCAGAAGCA AAATGACACC CAAAAGAAAG ACTTCTCTAA AAACCAAGGA 
AGTGGGCTGT CACCAAATGG AGCAGGGGAA GGACAAGGGC CTAAGAAGCA GACCAGGTTG 
GGTCTTGAGG CCAGAAAAGA AGACAGTCTT GCAGATTGGT ATTCTCAAGT CATCACAAAG 
TCAGAAATGA TTGAATACCA TGATGTAAGT GGCTGCTATA TCCTTCGTCC CTGGGCATAT 
TCCATTTGGG AATCCATCAA GGACTTTTTT GATGCTGAGA TCAAGAAACT CGGTGTTGAA 
AACTGCTATT TCCCCATGTT TGTGTCTCAA GGTGCATTAG AGAAAGAGAA GACTCATATT 
GCTGACTTTG CTCCTGAGGT TGCTTGGGTG ACAAGATCTG GCAAAACAGA GTTGGCAGAA 
CCAATTGCCA TTCGTCCTAC CAGTGAAACA GTAATGTATC CTGCATATGC GAAGTGGGTG 
CAGTCCCACA GAGACCTGCC CATAAAGCTC AACCAGTGGT GCAACGTGGT GCGTTGGGAG 
TTCAAGCACC CTCAGCCTTT CTTACGTACT CGTGAATTCC TCTGGCAGGA AGGGCACAGT 
GCCTTTGCTA CATTTGAAGA GGCAGCAGAA GAGGTTTTGC AGATACTTGA CTTATACGCT 
CAGGTATATG AAGAACTCCT GGCAATTCCT GTTGTAAAGG GAAGAAAGAC TGAAAAGGAG 
AAATTTGCGG GAGGAGATTA CACCACTACA GTGGAAGCAT TTATATCTGC CAGTGGAAGA 
GCTATCCAGG GAGCAACATC ACATCATTTA GGACAGAATT TCTCGAAAAT GTTTGAAATC 
GTTTTTGAAG ATCCAAAGAC AGCAGGAGAG AAGCAGTTGG CCTATCAGAA CTCCTGGGGC 
CTGACGACTC GAACTATCGG TGTGATGACC ATGGTTCACG GGGACAACGT GGGCTTAGTG 
CTGCCGCCCC GTGTAGCCTG TGTTCAGGTG GTGGTTATTC CCTGTGGCAT CACAAATGCA 
CTTTCTGAAG AAGACAGGGA TGCTCTACTT GCAAAATGCA ATGATTATAG AAGGCGGTTA 
CTCAGTGTCA ATATTCGAGT TCGAGTTGAT TTGCGAGATA ATTACTCACC AGGTTGGAAA 
TTCAATCACT GGGAACTCAA GGGAGTCCCG ATTAGACTTG AAGTTGGGCC ACGTGATATG 
AAGAACTGCC AGTTTGTAGC TGTCAGACGA GACACTGGGG AAAAGCTGAC AATTGCTGAA 
AATGAGGCTG AGACGAAGCT TCGTGCTATT TTGGAAGACA TCCACACTAA CCTCTTTACC 
CGGGCTTCTG AAGACCTTAA GACTCATATG GTTGTGGCTA ATACAATGGA GGACTTCCAG 
AAGGAGCTCG ATTCTGGGAA GATTGCTCAG ATTCCATTCT GTGGGGAAAT TGACTGTGAA 
GATTGGATCA AAAAGACTAC TGCCAGGGAT CAAGATCTTG AGCCCGGTGC TCCGTCGATG 
GGAGCTAAAA GCCTGTGCAT TCCCTTCAAG CCACTCTGCG AGCTGCCGCC AGGAGCCAAG 
TGTGTGTGTG GAAAGAACCC TGCCAAGTAC TACACCTTGT TCGGTCGTAG TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

EPRS cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

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